AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70215


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70215.t1 MTR_8g093770 100.000 143 0 0 1 143 1 143 1.51e-102 290
MS.gene70215.t1 MTR_5g012890 96.000 125 5 0 19 143 18 142 1.01e-86 249
MS.gene70215.t1 MTR_4g006830 88.889 144 15 1 1 143 1 144 2.91e-83 241
MS.gene70215.t1 MTR_5g012890 91.111 90 8 0 19 108 18 107 1.38e-56 172
MS.gene70215.t1 MTR_4g073510 66.667 54 17 1 1 53 1 54 3.27e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70215.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene70215.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene70215.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene70215.t1 AT1G15930 74.400 125 31 1 19 143 21 144 3.49e-64 192
MS.gene70215.t1 AT1G15930 74.400 125 31 1 19 143 21 144 3.49e-64 192

Find 33 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCAACTTGACGTAATC+AGG 0.299059 4.1:-8319489 None:intergenic
TCTCCAAGGGTCTTTGCATT+TGG 0.313809 4.1:-8319552 None:intergenic
GTTGCTGCAGCTGGAATTCC+AGG 0.331115 4.1:+8319318 MS.gene70215:CDS
AATCAACTTGCAATTATGTC+TGG 0.359633 4.1:+8318700 None:intergenic
ATTCCAGCTGCAGCAACAAC+TGG 0.406602 4.1:-8319312 None:intergenic
TGCAAAGACCCTTGGAGAAT+GGG 0.412944 4.1:+8319557 MS.gene70215:CDS
ATAATATCCATTGGCTCCCC+TGG 0.419454 4.1:-8319336 None:intergenic
GAGCCAGTTGTTGCTGCAGC+TGG 0.425162 4.1:+8319309 MS.gene70215:CDS
TTGAGAAAGTCACGCGCTTA+TGG 0.428624 4.1:+8319378 MS.gene70215:CDS
GACACTGTGTTCAGCACAAA+GGG 0.447539 4.1:-8319514 None:intergenic
ATGCAAAGACCCTTGGAGAA+TGG 0.451113 4.1:+8319556 MS.gene70215:CDS
GGAAAAGCTAGGAAGGTAAC+TGG 0.463250 4.1:+8320278 MS.gene70215:CDS
TTGCTCCTGTGTGGTTGTCA+AGG 0.525394 4.1:+8320301 MS.gene70215:CDS
GTTCCAAATGCAAAGACCCT+TGG 0.532260 4.1:+8319549 MS.gene70215:CDS
TTGCTGCAGCTGGAATTCCA+GGG 0.537879 4.1:+8319319 MS.gene70215:CDS
ATCAACTTGCAATTATGTCT+GGG 0.539041 4.1:+8318701 None:intergenic
TTGTGCAAAATTGATTCAGA+AGG 0.565495 4.1:+8320257 MS.gene70215:CDS
ACTGCAGTCATAATATCCAT+TGG 0.568889 4.1:-8319345 None:intergenic
TGCTGCAGCTGGAATTCCAG+GGG 0.575795 4.1:+8319320 MS.gene70215:CDS
TTGATTCAGAAGGAAAAGCT+AGG 0.578774 4.1:+8320267 MS.gene70215:CDS
ACAGCTCTGTGTTCTTGCTG+AGG 0.587565 4.1:+8319455 MS.gene70215:CDS
CCAACCTGATTACGTCAAGT+TGG 0.591510 4.1:+8319485 MS.gene70215:CDS
TTACACCAGCCCATTCTCCA+AGG 0.592149 4.1:-8319566 None:intergenic
TTCAGAAGGAAAAGCTAGGA+AGG 0.596975 4.1:+8320271 MS.gene70215:CDS
TGGAATTCCAGGGGAGCCAA+TGG 0.602144 4.1:+8319329 MS.gene70215:CDS
AGAAAGTCACGCGCTTATGG+TGG 0.613205 4.1:+8319381 MS.gene70215:CDS
CCAACTTGACGTAATCAGGT+TGG 0.614249 4.1:-8319485 None:intergenic
AAGACCCTTGGAGAATGGGC+TGG 0.615483 4.1:+8319561 MS.gene70215:CDS
TGACACTGTGTTCAGCACAA+AGG 0.628583 4.1:-8319515 None:intergenic
GCTTATGGTGGTCTTGCACG+TGG 0.635715 4.1:+8319393 MS.gene70215:CDS
GGTAACTGGTTGCTCCTGTG+TGG 0.641819 4.1:+8320292 MS.gene70215:CDS
TACACCAGCCCATTCTCCAA+GGG 0.643748 4.1:-8319565 None:intergenic
ACATACCTTGACAACCACAC+AGG 0.682837 4.1:-8320306 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATAATTGAGAATTG+TGG - chr4.1:8319647-8319666 None:intergenic 15.0%
!! CATATAGATTAAAATAAATG+AGG - chr4.1:8320129-8320148 None:intergenic 15.0%
!! TATAAAATGTTATTGCTTAT+TGG - chr4.1:8319790-8319809 None:intergenic 15.0%
!!! CATGAATTACTTTAAAATAA+GGG + chr4.1:8319678-8319697 MS.gene70215:intron 15.0%
!!! TCATGAATTACTTTAAAATA+AGG + chr4.1:8319677-8319696 MS.gene70215:intron 15.0%
!!! TTAACTATTTTCATTTTTGT+AGG + chr4.1:8320385-8320404 MS.gene70215:intron 15.0%
!!! TTATAATAACTTTTTGATCT+GGG - chr4.1:8319096-8319115 None:intergenic 15.0%
!!! TTTATAATAACTTTTTGATC+TGG - chr4.1:8319097-8319116 None:intergenic 15.0%
!! AATAGTTAAGCATACATAAA+CGG - chr4.1:8320374-8320393 None:intergenic 20.0%
!! ATTGATTATGAGGATAATTA+TGG + chr4.1:8319044-8319063 MS.gene70215:intron 20.0%
!! CAAAACCAATATAAAACAAA+AGG - chr4.1:8320039-8320058 None:intergenic 20.0%
!!! AATTACTTTAAAATAAGGGT+GGG + chr4.1:8319682-8319701 MS.gene70215:intron 20.0%
!!! ATTTGCCTTTTGTTTTATAT+TGG + chr4.1:8320031-8320050 MS.gene70215:intron 20.0%
!!! TTTTCATTTTTGTAGGATTT+CGG + chr4.1:8320392-8320411 MS.gene70215:intron 20.0%
! AAATTCAGAAGTTATTGTCT+TGG + chr4.1:8320165-8320184 MS.gene70215:intron 25.0%
! ACAAAAAGTTTCTCTATAGT+CGG - chr4.1:8319179-8319198 None:intergenic 25.0%
! CTATGTTTATAGTAATGCAA+GGG - chr4.1:8319595-8319614 None:intergenic 25.0%
! GACAATAACTTCTGAATTTA+AGG - chr4.1:8320164-8320183 None:intergenic 25.0%
! TCAAATATGTCATAAGGAAA+TGG - chr4.1:8319624-8319643 None:intergenic 25.0%
! TCTATGTTTATAGTAATGCA+AGG - chr4.1:8319596-8319615 None:intergenic 25.0%
!!! AGTACTGATATTGAAATAGT+TGG + chr4.1:8318888-8318907 MS.gene70215:intron 25.0%
!!! GAATTACTTTAAAATAAGGG+TGG + chr4.1:8319681-8319700 MS.gene70215:intron 25.0%
ATTTCTTATAGTTACTACCG+AGG - chr4.1:8318930-8318949 None:intergenic 30.0%
CGGTTTCATTAATTGCTTAT+TGG - chr4.1:8319763-8319782 None:intergenic 30.0%
GAATCCAAACATTTCTATTC+CGG - chr4.1:8320339-8320358 None:intergenic 30.0%
TCATTTCATGCATAACAGTT+AGG - chr4.1:8319848-8319867 None:intergenic 30.0%
TGTTGTTCAGTTATTGTTTC+TGG + chr4.1:8319963-8319982 MS.gene70215:intron 30.0%
TTGTGGTCAAATATGTCATA+AGG - chr4.1:8319630-8319649 None:intergenic 30.0%
TTTCTTATAGTTACTACCGA+GGG - chr4.1:8318929-8318948 None:intergenic 30.0%
! AACTTCTGAATTTAAGGCAA+AGG - chr4.1:8320158-8320177 None:intergenic 30.0%
! TGTGAGATTCTACTTGTTAA+TGG + chr4.1:8318753-8318772 MS.gene70215:intron 30.0%
! TTGTGCAAAATTGATTCAGA+AGG + chr4.1:8320257-8320276 MS.gene70215:CDS 30.0%
!! AACTTTTTGATCTGGGATTA+AGG - chr4.1:8319089-8319108 None:intergenic 30.0%
!! AAGGTTGTACACTATTTCTA+TGG - chr4.1:8319899-8319918 None:intergenic 30.0%
!! TGTACACTATTTCTATGGAT+TGG - chr4.1:8319894-8319913 None:intergenic 30.0%
ACAAGTAGAATCTCACAAAC+AGG - chr4.1:8318750-8318769 None:intergenic 35.0%
ACTGCAGTCATAATATCCAT+TGG - chr4.1:8319348-8319367 None:intergenic 35.0%
ATGGTCAAATGAAGCTAATG+AGG + chr4.1:8319063-8319082 MS.gene70215:intron 35.0%
TCATCCGGAATAGAAATGTT+TGG + chr4.1:8320332-8320351 MS.gene70215:intron 35.0%
TTGATTCAGAAGGAAAAGCT+AGG + chr4.1:8320267-8320286 MS.gene70215:CDS 35.0%
TTTCATGCATAACAGTTAGG+AGG - chr4.1:8319845-8319864 None:intergenic 35.0%
! AATTTTGCACAACTGCATGA+AGG - chr4.1:8320249-8320268 None:intergenic 35.0%
! CATTGCGATCATTGATTATG+AGG + chr4.1:8319034-8319053 MS.gene70215:intron 35.0%
! GTCAAGGTATGTTTTTCATC+CGG + chr4.1:8320317-8320336 MS.gene70215:intron 35.0%
! TGTTATTGCTTATTGGTGCT+CGG - chr4.1:8319783-8319802 None:intergenic 35.0%
!! ACACTATTTCTATGGATTGG+AGG - chr4.1:8319891-8319910 None:intergenic 35.0%
!! ATTGTTTCTGGTGTATTCCT+TGG + chr4.1:8319975-8319994 MS.gene70215:intron 35.0%
!! TTGTTTCTGGTGTATTCCTT+GGG + chr4.1:8319976-8319995 MS.gene70215:intron 35.0%
AAAAACTCAAGACGCACACA+TGG - chr4.1:8319216-8319235 None:intergenic 40.0%
ATTGGCACCTCTCATGATTA+GGG - chr4.1:8319745-8319764 None:intergenic 40.0%
CAACCTTTGAATGTCAAGAG+CGG + chr4.1:8319912-8319931 MS.gene70215:intron 40.0%
TATTGGCACCTCTCATGATT+AGG - chr4.1:8319746-8319765 None:intergenic 40.0%
TTCACCAACTTGACGTAATC+AGG - chr4.1:8319492-8319511 None:intergenic 40.0%
TTCAGAAGGAAAAGCTAGGA+AGG + chr4.1:8320271-8320290 MS.gene70215:CDS 40.0%
! ATTTTTGTAGGATTTCGGCG+AGG + chr4.1:8320397-8320416 MS.gene70215:intron 40.0%
ACAGAGTTCAGAGAGGATAC+AGG - chr4.1:8320104-8320123 None:intergenic 45.0%
ACATACCTTGACAACCACAC+AGG - chr4.1:8320309-8320328 None:intergenic 45.0%
AGAGAGGATACAGGGTAAGA+GGG - chr4.1:8320095-8320114 None:intergenic 45.0%
ATCCTGACCCTAATCATGAG+AGG + chr4.1:8319735-8319754 MS.gene70215:intron 45.0%
ATGCAAAGACCCTTGGAGAA+TGG + chr4.1:8319556-8319575 MS.gene70215:CDS 45.0%
CAGAGTTCAGAGAGGATACA+GGG - chr4.1:8320103-8320122 None:intergenic 45.0%
CCAACCTGATTACGTCAAGT+TGG + chr4.1:8319485-8319504 MS.gene70215:CDS 45.0%
CCAACTTGACGTAATCAGGT+TGG - chr4.1:8319488-8319507 None:intergenic 45.0%
GACACTGTGTTCAGCACAAA+GGG - chr4.1:8319517-8319536 None:intergenic 45.0%
GATCTGATAGTCATAGCCCT+CGG + chr4.1:8318910-8318929 MS.gene70215:intron 45.0%
GATTTGCACCACGATGTTGA+GGG - chr4.1:8319718-8319737 None:intergenic 45.0%
GGAAAAGCTAGGAAGGTAAC+TGG + chr4.1:8320278-8320297 MS.gene70215:CDS 45.0%
GTTCCAAATGCAAAGACCCT+TGG + chr4.1:8319549-8319568 MS.gene70215:CDS 45.0%
TCTCCAAGGGTCTTTGCATT+TGG - chr4.1:8319555-8319574 None:intergenic 45.0%
TGACACTGTGTTCAGCACAA+AGG - chr4.1:8319518-8319537 None:intergenic 45.0%
TGAGGTTACAGAGTTCAGAG+AGG - chr4.1:8320111-8320130 None:intergenic 45.0%
TGCAAAGACCCTTGGAGAAT+GGG + chr4.1:8319557-8319576 MS.gene70215:CDS 45.0%
TTGAGAAAGTCACGCGCTTA+TGG + chr4.1:8319378-8319397 MS.gene70215:CDS 45.0%
!! ATAATATCCATTGGCTCCCC+TGG - chr4.1:8319339-8319358 None:intergenic 45.0%
!!! AAATTAAAAAATTTAATTTG+TGG + chr4.1:8319008-8319027 MS.gene70215:intron 5.0%
ACAGCTCTGTGTTCTTGCTG+AGG + chr4.1:8319455-8319474 MS.gene70215:CDS 50.0%
AGAAAGTCACGCGCTTATGG+TGG + chr4.1:8319381-8319400 MS.gene70215:CDS 50.0%
ATTCCAGCTGCAGCAACAAC+TGG - chr4.1:8319315-8319334 None:intergenic 50.0%
CACCTCTCATGATTAGGGTC+AGG - chr4.1:8319740-8319759 None:intergenic 50.0%
CAGAGAGGATACAGGGTAAG+AGG - chr4.1:8320096-8320115 None:intergenic 50.0%
CCACCGCTCTTGACATTCAA+AGG - chr4.1:8319918-8319937 None:intergenic 50.0%
CCTTTGAATGTCAAGAGCGG+TGG + chr4.1:8319915-8319934 MS.gene70215:intron 50.0%
CTGGTGTATTCCTTGGGTCT+GGG + chr4.1:8319982-8320001 MS.gene70215:intron 50.0%
GAGAGGATACAGGGTAAGAG+GGG - chr4.1:8320094-8320113 None:intergenic 50.0%
GGATTTGCACCACGATGTTG+AGG - chr4.1:8319719-8319738 None:intergenic 50.0%
TACACCAGCCCATTCTCCAA+GGG - chr4.1:8319568-8319587 None:intergenic 50.0%
TATAGTCGGCGAGATTCACG+TGG - chr4.1:8319165-8319184 None:intergenic 50.0%
TCTGGTGTATTCCTTGGGTC+TGG + chr4.1:8319981-8320000 MS.gene70215:intron 50.0%
TGACATCTCCCTCAACATCG+TGG + chr4.1:8319707-8319726 MS.gene70215:intron 50.0%
TGGTGTATTCCTTGGGTCTG+GGG + chr4.1:8319983-8320002 MS.gene70215:intron 50.0%
TTACACCAGCCCATTCTCCA+AGG - chr4.1:8319569-8319588 None:intergenic 50.0%
TTGCTCCTGTGTGGTTGTCA+AGG + chr4.1:8320301-8320320 MS.gene70215:CDS 50.0%
TTGCTGCAGCTGGAATTCCA+GGG + chr4.1:8319319-8319338 MS.gene70215:CDS 50.0%
AAGACCCTTGGAGAATGGGC+TGG + chr4.1:8319561-8319580 MS.gene70215:CDS 55.0%
GGTAACTGGTTGCTCCTGTG+TGG + chr4.1:8320292-8320311 MS.gene70215:CDS 55.0%
GTTGCTGCAGCTGGAATTCC+AGG + chr4.1:8319318-8319337 MS.gene70215:CDS 55.0%
TGCTGCAGCTGGAATTCCAG+GGG + chr4.1:8319320-8319339 MS.gene70215:CDS 55.0%
TGGAATTCCAGGGGAGCCAA+TGG + chr4.1:8319329-8319348 MS.gene70215:CDS 55.0%
TGTATTCCTTGGGTCTGGGG+AGG + chr4.1:8319986-8320005 MS.gene70215:intron 55.0%
! GCTTATGGTGGTCTTGCACG+TGG + chr4.1:8319393-8319412 MS.gene70215:CDS 55.0%
GAGCCAGTTGTTGCTGCAGC+TGG + chr4.1:8319309-8319328 MS.gene70215:CDS 60.0%
AACCAGCCTCCCCAGACCCA+AGG - chr4.1:8319995-8320014 None:intergenic 65.0%
TTCCTTGGGTCTGGGGAGGC+TGG + chr4.1:8319990-8320009 MS.gene70215:intron 65.0%
! TGGGTCTGGGGAGGCTGGTT+CGG + chr4.1:8319995-8320014 MS.gene70215:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 8318715 8320466 8318715 ID=MS.gene70215
chr4.1 mRNA 8318715 8320466 8318715 ID=MS.gene70215.t1;Parent=MS.gene70215
chr4.1 exon 8318715 8318722 8318715 ID=MS.gene70215.t1.exon1;Parent=MS.gene70215.t1
chr4.1 CDS 8318715 8318722 8318715 ID=cds.MS.gene70215.t1;Parent=MS.gene70215.t1
chr4.1 exon 8319287 8319584 8319287 ID=MS.gene70215.t1.exon2;Parent=MS.gene70215.t1
chr4.1 CDS 8319287 8319584 8319287 ID=cds.MS.gene70215.t1;Parent=MS.gene70215.t1
chr4.1 exon 8320257 8320322 8320257 ID=MS.gene70215.t1.exon3;Parent=MS.gene70215.t1
chr4.1 CDS 8320257 8320322 8320257 ID=cds.MS.gene70215.t1;Parent=MS.gene70215.t1
chr4.1 exon 8320407 8320466 8320407 ID=MS.gene70215.t1.exon4;Parent=MS.gene70215.t1
chr4.1 CDS 8320407 8320466 8320407 ID=cds.MS.gene70215.t1;Parent=MS.gene70215.t1
Gene Sequence

>MS.gene70215

ATGTCTGGGTATGTCTATTTTTGCTTAATTTACCTGTTTGTGAGATTCTACTTGTTAATGGTCTATTCATTATCTTATTTTGCAATTTTTTGTTGAATCTATATAGTTTTATTTTCTTTCATGAACTGCGTTTGTAAACTTTCAAATGCATTATAGATTTGATTTTAATTTTAAGTACTGATATTGAAATAGTTGGATCTGATAGTCATAGCCCTCGGTAGTAACTATAAGAAATTTAGAACACAGATAACAGTTTAGTATTTATTTAATTTTCAAAACTATTTTTCAAAATGAAATTAAAAAATTTAATTTGTGGTTGCATTGCGATCATTGATTATGAGGATAATTATGGTCAAATGAAGCTAATGAGGCCTTAATCCCAGATCAAAAAGTTATTATAAACTGCTGTGTATTAGTGTAGACACTTTTCTGTTTCTGTGATTTGAACCACGTGAATCTCGCCGACTATAGAGAAACTTTTTGTTGTGCAATGCTTATCCATGTGTGCGTCTTGAGTTTTTAAGATATTCATTGTTAATTTTCTGACTGTTGATAAACATTTTGTTGTTCAGAGAAGAAGAAGTTGTTGTAGCTGAGCCAGTTGTTGCTGCAGCTGGAATTCCAGGGGAGCCAATGGATATTATGACTGCAGTTCAGCTTGTGTTGAGAAAGTCACGCGCTTATGGTGGTCTTGCACGTGGTCTTCATGAAAGTGCTAAAGTGATTGAGAAGCATGCTGCACAGCTCTGTGTTCTTGCTGAGGATTGTGACCAACCTGATTACGTCAAGTTGGTGAAAGCCCTTTGTGCTGAACACAGTGTCAGTTTGTTGACTGTTCCAAATGCAAAGACCCTTGGAGAATGGGCTGGTGTAAGTGCCCTTGCATTACTATAAACATAGATCAATCCATTTCCTTATGACATATTTGACCACAATTCTCAATTATATATTTTTTGATAAATTCATGAATTACTTTAAAATAAGGGTGGGAGTGACATCTCCCTCAACATCGTGGTGCAAATCCTGACCCTAATCATGAGAGGTGCCAATAAGCAATTAATGAAACCGAGCACCAATAAGCAATAACATTTTATAAATTGCTTCTCTATAGATGTGACTTACTTAAACCTCCTAACTGTTATGCATGAAATGATAAATCTTTCTCATCAGACTCCTCCAATCCATAGAAATAGTGTACAACCTTTGAATGTCAAGAGCGGTGGACAGTTCTCAAATTTAGATTATGAATGTTGTTCAGTTATTGTTTCTGGTGTATTCCTTGGGTCTGGGGAGGCTGGTTCGGCAGCTTGTACTGTATTTGCCTTTTGTTTTATATTGGTTTTGTTTTTCTTTGTGATGTGTTTCTGTTTAGAGTACCCCTCTTACCCTGTATCCTCTCTGAACTCTGTAACCTCATTTATTTTAATCTATATGTTTATACCTTTGCCTTAAATTCAGAAGTTATTGTCTTGGTCTTTTTTTTATGCTTTCAAAATCTTATGTCATTCTTTTGTACTGATTTTGTGACGCACCTTCATGCAGTTGTGCAAAATTGATTCAGAAGGAAAAGCTAGGAAGGTAACTGGTTGCTCCTGTGTGGTTGTCAAGGTATGTTTTTCATCCGGAATAGAAATGTTTGGATTCTGTTTTCATCTTCCGTTTATGTATGCTTAACTATTTTCATTTTTGTAGGATTTCGGCGAGGAACATGAAGCATACAACGTTGTTCTTCAGCACGTGAAATCTAACTGA

Protein sequence

>MS.gene70215.t1

MSGEEEVVVAEPVVAAAGIPGEPMDIMTAVQLVLRKSRAYGGLARGLHESAKVIEKHAAQLCVLAEDCDQPDYVKLVKALCAEHSVSLLTVPNAKTLGEWAGLCKIDSEGKARKVTGCSCVVVKDFGEEHEAYNVVLQHVKSN