AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28384


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28384.t1 MTR_8g093770 100.000 143 0 0 1 143 1 143 1.51e-102 290
MS.gene28384.t1 MTR_5g012890 96.000 125 5 0 19 143 18 142 1.01e-86 249
MS.gene28384.t1 MTR_4g006830 88.889 144 15 1 1 143 1 144 2.91e-83 241
MS.gene28384.t1 MTR_5g012890 91.111 90 8 0 19 108 18 107 1.38e-56 172
MS.gene28384.t1 MTR_4g073510 66.667 54 17 1 1 53 1 54 3.27e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28384.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene28384.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene28384.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene28384.t1 AT1G15930 74.400 125 31 1 19 143 21 144 3.49e-64 192
MS.gene28384.t1 AT1G15930 74.400 125 31 1 19 143 21 144 3.49e-64 192

Find 37 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCAACTTGACGTAATC+AGG 0.299059 4.4:+9281203 None:intergenic
TCTCCAAGGGTCTTTGCATT+TGG 0.313809 4.4:+9281140 None:intergenic
GTTGCTGCAGCTGGAATTCC+AGG 0.331115 4.4:-9281374 MS.gene28384:CDS
AATCAACTTGCAATTATGTC+TGG 0.359633 4.4:-9281778 None:intergenic
ATTCCAGCTGCAGCAACAAC+TGG 0.406602 4.4:+9281380 None:intergenic
TGCAAAGACCCTTGGAGAAT+GGG 0.412944 4.4:-9281135 MS.gene28384:CDS
ATAATGTCCATTGGCTCCCC+TGG 0.424358 4.4:+9281356 None:intergenic
GAGCCAGTTGTTGCTGCAGC+TGG 0.425162 4.4:-9281383 MS.gene28384:CDS
GACACTGTGTTCAGCACAAA+GGG 0.447539 4.4:+9281178 None:intergenic
ATGCAAAGACCCTTGGAGAA+TGG 0.451113 4.4:-9281136 MS.gene28384:CDS
TTGAGAAAGTCGCGGGCATA+TGG 0.459751 4.4:-9281314 MS.gene28384:CDS
GGAAAAGCTAGGAAGGTAAC+TGG 0.463250 4.4:-9280397 MS.gene28384:CDS
TTGCTCCTGTGTGGTTGTCA+AGG 0.525394 4.4:-9280374 MS.gene28384:intron
GTTCCAAATGCAAAGACCCT+TGG 0.532260 4.4:-9281143 MS.gene28384:CDS
TTGCTGCAGCTGGAATTCCA+GGG 0.537879 4.4:-9281373 MS.gene28384:CDS
ATCAACTTGCAATTATGTCT+GGG 0.539041 4.4:-9281777 None:intergenic
TTGTGCAAAATTGATTCAGA+AGG 0.565495 4.4:-9280418 MS.gene28384:CDS
GCGACTTTCTCAATACAAGC+TGG 0.568533 4.4:+9281324 None:intergenic
TGCTGCAGCTGGAATTCCAG+GGG 0.575795 4.4:-9281372 MS.gene28384:CDS
TTGATTCAGAAGGAAAAGCT+AGG 0.578774 4.4:-9280408 MS.gene28384:CDS
ACAGCTCTGTGTTCTTGCTG+AGG 0.587565 4.4:-9281237 MS.gene28384:CDS
CCAACCTGATTACGTCAAGT+TGG 0.591510 4.4:-9281207 MS.gene28384:CDS
TTACACCAGCCCATTCTCCA+AGG 0.592149 4.4:+9281126 None:intergenic
TTCAGAAGGAAAAGCTAGGA+AGG 0.596975 4.4:-9280404 MS.gene28384:CDS
TGGAATTCCAGGGGAGCCAA+TGG 0.597407 4.4:-9281363 MS.gene28384:CDS
GCTTGTATTGAGAAAGTCGC+GGG 0.598261 4.4:-9281321 MS.gene28384:CDS
ACAGCGGTCATAATGTCCAT+TGG 0.598980 4.4:+9281347 None:intergenic
CCAACTTGACGTAATCAGGT+TGG 0.614249 4.4:+9281207 None:intergenic
AGAAAGTCGCGGGCATATGG+TGG 0.615038 4.4:-9281311 MS.gene28384:CDS
GCATATGGTGGTCTTGCACG+AGG 0.615278 4.4:-9281299 MS.gene28384:CDS
AAGACCCTTGGAGAATGGGC+TGG 0.615483 4.4:-9281131 MS.gene28384:CDS
TGACACTGTGTTCAGCACAA+AGG 0.628583 4.4:+9281177 None:intergenic
GGTAACTGGTTGCTCCTGTG+TGG 0.641819 4.4:-9280383 MS.gene28384:CDS
TACACCAGCCCATTCTCCAA+GGG 0.643748 4.4:+9281127 None:intergenic
ACATACCTTGACAACCACAC+AGG 0.682837 4.4:+9280369 None:intergenic
TCTCAATACAAGCTGGACAG+CGG 0.712950 4.4:+9281331 None:intergenic
AGCTTGTATTGAGAAAGTCG+CGG 0.717989 4.4:-9281322 MS.gene28384:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACTTCTTAATAATTTA+AGG + chr4.4:9281482-9281501 None:intergenic 10.0%
!! AAATATATAATTGAGAATTG+TGG + chr4.4:9280951-9280970 None:intergenic 15.0%
!!! AATTACTTTAAAATAAGTGT+GGG - chr4.4:9280986-9281005 MS.gene28384:intron 15.0%
!!! TTAACTATTTTCATTTTTGT+AGG - chr4.4:9281704-9281723 MS.gene28384:intron 15.0%
!! TTAATCTAGGTTTATAGTAA+TGG + chr4.4:9280902-9280921 None:intergenic 20.0%
!! TTATTAAGAAGTTATTGTCT+TGG - chr4.4:9281486-9281505 MS.gene28384:intron 20.0%
!!! GAATTACTTTAAAATAAGTG+TGG - chr4.4:9280985-9281004 MS.gene28384:intron 20.0%
!!! TTTTCATTTTTGTAGGATTT+CGG - chr4.4:9281711-9281730 MS.gene28384:intron 20.0%
! AGGTTTCATTAATTCCTTAT+TGG + chr4.4:9281067-9281086 None:intergenic 25.0%
! AGTACTACTATTGAAATAGT+TGG - chr4.4:9280405-9280424 MS.gene28384:CDS 25.0%
! ATAGAGAAGCAATTATTTGT+TGG + chr4.4:9281121-9281140 None:intergenic 25.0%
! GGCAAAGATTAAAATAAATG+AGG + chr4.4:9281461-9281480 None:intergenic 25.0%
!! TGTTGTTTAGTTATTGTTTC+TGG - chr4.4:9281278-9281297 MS.gene28384:CDS 25.0%
!!! ACTATTTTTTGTGTCTTGAA+TGG - chr4.4:9280510-9280529 MS.gene28384:intron 25.0%
!!! CTATTTTTTGTGTCTTGAAT+GGG - chr4.4:9280511-9280530 MS.gene28384:intron 25.0%
AATTGAGAATTGTGGTCAAA+TGG + chr4.4:9280943-9280962 None:intergenic 30.0%
CAGAGTACAAGATAAAACAA+AGG + chr4.4:9281354-9281373 None:intergenic 30.0%
TGGATTTCTTAGACAACAAA+AGG + chr4.4:9281101-9281120 None:intergenic 30.0%
TTTCTTAGACAACAAAAGGT+TGG + chr4.4:9281097-9281116 None:intergenic 30.0%
! CTAGGTTTATAGTAATGGAA+GGG + chr4.4:9280897-9280916 None:intergenic 30.0%
! TCATCTGGAATAGAAATGTT+TGG - chr4.4:9281651-9281670 MS.gene28384:intron 30.0%
! TCTAGGTTTATAGTAATGGA+AGG + chr4.4:9280898-9280917 None:intergenic 30.0%
! TGTGAGATTCTACTTGTTAA+TGG - chr4.4:9280269-9280288 MS.gene28384:CDS 30.0%
! TTGTGCAAAATTGATTCAGA+AGG - chr4.4:9281576-9281595 MS.gene28384:intron 30.0%
!! ACAAGGAAATGGATTAATCT+AGG + chr4.4:9280915-9280934 None:intergenic 30.0%
AAATGGATGTCACAAGGAAA+TGG + chr4.4:9280926-9280945 None:intergenic 35.0%
ACAAGTAGAATCTCACAAAC+AGG + chr4.4:9280266-9280285 None:intergenic 35.0%
ACTTCTTATAGTTACTACCG+AGG + chr4.4:9280448-9280467 None:intergenic 35.0%
AGTTAAGCATACGTAAACAG+AGG + chr4.4:9281690-9281709 None:intergenic 35.0%
CTTCTTATAGTTACTACCGA+GGG + chr4.4:9280447-9280466 None:intergenic 35.0%
TCATTTCATGCATCACAGTT+AGG + chr4.4:9281165-9281184 None:intergenic 35.0%
TCCTCCAATCCATAGAAATA+GGG - chr4.4:9281204-9281223 MS.gene28384:CDS 35.0%
TTGATTCAGAAGGAAAAGCT+AGG - chr4.4:9281586-9281605 MS.gene28384:intron 35.0%
! AATTTTGCACAACTGCATGA+AGG + chr4.4:9281568-9281587 None:intergenic 35.0%
! CATTTTCTTCTTCAGAGAAG+AGG - chr4.4:9280574-9280593 MS.gene28384:intron 35.0%
! GTCAAGGTATGTTTTTCATC+TGG - chr4.4:9281636-9281655 MS.gene28384:intron 35.0%
!! ATTGTTTCTGGTGTATTCCT+TGG - chr4.4:9281290-9281309 MS.gene28384:CDS 35.0%
!! TGTACCCTATTTCTATGGAT+TGG + chr4.4:9281211-9281230 None:intergenic 35.0%
!! TTGTTTCTGGTGTATTCCTT+GGG - chr4.4:9281291-9281310 MS.gene28384:CDS 35.0%
AAATCCTGACCCTAATCATG+AGG - chr4.4:9281037-9281056 MS.gene28384:intron 40.0%
AATCCTGACCCTAATCATGA+GGG - chr4.4:9281038-9281057 MS.gene28384:intron 40.0%
AGAAAAACAAAATCACCCCC+AGG + chr4.4:9281382-9281401 None:intergenic 40.0%
AGCTTGTATTGAGAAAGTCG+CGG - chr4.4:9280672-9280691 MS.gene28384:intron 40.0%
CTCCTCCAATCCATAGAAAT+AGG - chr4.4:9281203-9281222 MS.gene28384:CDS 40.0%
GATTTGCACCATGATGTTGA+GGG + chr4.4:9281022-9281041 None:intergenic 40.0%
TAACATCTCCCTCAACATCA+TGG - chr4.4:9281011-9281030 MS.gene28384:intron 40.0%
TACAACCCTTGAATGTCAAG+CGG - chr4.4:9281227-9281246 MS.gene28384:CDS 40.0%
TTCACCAACTTGACGTAATC+AGG + chr4.4:9280794-9280813 None:intergenic 40.0%
TTCAGAAGGAAAAGCTAGGA+AGG - chr4.4:9281590-9281609 MS.gene28384:intron 40.0%
TTCAGAGAGGATACAGAGTA+GGG + chr4.4:9281430-9281449 None:intergenic 40.0%
TTTCATGCATCACAGTTAGG+AGG + chr4.4:9281162-9281181 None:intergenic 40.0%
! AACAAAAGGTTGGTCACAAG+AGG + chr4.4:9281087-9281106 None:intergenic 40.0%
! ATTTTTGTAGGATTTCGGCG+AGG - chr4.4:9281716-9281735 MS.gene28384:intron 40.0%
!! ACCCTATTTCTATGGATTGG+AGG + chr4.4:9281208-9281227 None:intergenic 40.0%
!! AGGGTTGTACCCTATTTCTA+TGG + chr4.4:9281216-9281235 None:intergenic 40.0%
ACAGCGGTCATAATGTCCAT+TGG + chr4.4:9280650-9280669 None:intergenic 45.0%
ACATACCTTGACAACCACAC+AGG + chr4.4:9281628-9281647 None:intergenic 45.0%
ATCCTGACCCTAATCATGAG+GGG - chr4.4:9281039-9281058 MS.gene28384:intron 45.0%
ATCTGATAGCCAATAGCCCT+CGG - chr4.4:9280428-9280447 MS.gene28384:intron 45.0%
ATGCAAAGACCCTTGGAGAA+TGG - chr4.4:9280858-9280877 MS.gene28384:intron 45.0%
CCAACCTGATTACGTCAAGT+TGG - chr4.4:9280787-9280806 MS.gene28384:intron 45.0%
CCAACTTGACGTAATCAGGT+TGG + chr4.4:9280790-9280809 None:intergenic 45.0%
CTGGTGTATTCCTTGGGTTT+GGG - chr4.4:9281297-9281316 MS.gene28384:CDS 45.0%
GACACTGTGTTCAGCACAAA+GGG + chr4.4:9280819-9280838 None:intergenic 45.0%
GCGACTTTCTCAATACAAGC+TGG + chr4.4:9280673-9280692 None:intergenic 45.0%
GCTTGTATTGAGAAAGTCGC+GGG - chr4.4:9280673-9280692 MS.gene28384:intron 45.0%
GGAAAAGCTAGGAAGGTAAC+TGG - chr4.4:9281597-9281616 MS.gene28384:intron 45.0%
GGATTTGCACCATGATGTTG+AGG + chr4.4:9281023-9281042 None:intergenic 45.0%
GTGGTCAAATGGATGTCACA+AGG + chr4.4:9280932-9280951 None:intergenic 45.0%
GTTCAGAGAGGATACAGAGT+AGG + chr4.4:9281431-9281450 None:intergenic 45.0%
GTTCCAAATGCAAAGACCCT+TGG - chr4.4:9280851-9280870 MS.gene28384:intron 45.0%
TAGTTACTACCGAGGGCTAT+TGG + chr4.4:9280440-9280459 None:intergenic 45.0%
TCTCAATACAAGCTGGACAG+CGG + chr4.4:9280666-9280685 None:intergenic 45.0%
TCTCCAAGGGTCTTTGCATT+TGG + chr4.4:9280857-9280876 None:intergenic 45.0%
TCTGGTGTATTCCTTGGGTT+TGG - chr4.4:9281296-9281315 MS.gene28384:CDS 45.0%
TGACACTGTGTTCAGCACAA+AGG + chr4.4:9280820-9280839 None:intergenic 45.0%
TGAGGTTACAGAGTTCAGAG+AGG + chr4.4:9281443-9281462 None:intergenic 45.0%
TGCAAAGACCCTTGGAGAAT+GGG - chr4.4:9280859-9280878 MS.gene28384:intron 45.0%
TGGTGTATTCCTTGGGTTTG+GGG - chr4.4:9281298-9281317 MS.gene28384:CDS 45.0%
AACCCTTGAATGTCAAGCGG+TGG - chr4.4:9281230-9281249 MS.gene28384:CDS 50.0%
ACAGCTCTGTGTTCTTGCTG+AGG - chr4.4:9280757-9280776 MS.gene28384:intron 50.0%
ATCTTGTACTCTGCTGCTCC+TGG - chr4.4:9281361-9281380 MS.gene28384:CDS 50.0%
ATTCCAGCTGCAGCAACAAC+TGG + chr4.4:9280617-9280636 None:intergenic 50.0%
ATTGGCGCCCCTCATGATTA+GGG + chr4.4:9281049-9281068 None:intergenic 50.0%
GTCCACCGCTTGACATTCAA+GGG + chr4.4:9281235-9281254 None:intergenic 50.0%
TACACCAGCCCATTCTCCAA+GGG + chr4.4:9280870-9280889 None:intergenic 50.0%
TATTGGCGCCCCTCATGATT+AGG + chr4.4:9281050-9281069 None:intergenic 50.0%
TCTTGTACTCTGCTGCTCCT+GGG - chr4.4:9281362-9281381 MS.gene28384:CDS 50.0%
TGTATTCCTTGGGTTTGGGG+AGG - chr4.4:9281301-9281320 MS.gene28384:CDS 50.0%
TGTCCACCGCTTGACATTCA+AGG + chr4.4:9281236-9281255 None:intergenic 50.0%
TTACACCAGCCCATTCTCCA+AGG + chr4.4:9280871-9280890 None:intergenic 50.0%
TTGAGAAAGTCGCGGGCATA+TGG - chr4.4:9280680-9280699 MS.gene28384:intron 50.0%
TTGCTCCTGTGTGGTTGTCA+AGG - chr4.4:9281620-9281639 MS.gene28384:intron 50.0%
TTGCTGCAGCTGGAATTCCA+GGG - chr4.4:9280621-9280640 MS.gene28384:intron 50.0%
!! AATCATGAGGGGCGCCAATA+AGG - chr4.4:9281050-9281069 MS.gene28384:intron 50.0%
!! ATAATGTCCATTGGCTCCCC+TGG + chr4.4:9280641-9280660 None:intergenic 50.0%
AAGACCCTTGGAGAATGGGC+TGG - chr4.4:9280863-9280882 MS.gene28384:intron 55.0%
AGAAAGTCGCGGGCATATGG+TGG - chr4.4:9280683-9280702 MS.gene28384:intron 55.0%
CTTGTACTCTGCTGCTCCTG+GGG - chr4.4:9281363-9281382 MS.gene28384:CDS 55.0%
GGTAACTGGTTGCTCCTGTG+TGG - chr4.4:9281611-9281630 MS.gene28384:intron 55.0%
GTTGCTGCAGCTGGAATTCC+AGG - chr4.4:9280620-9280639 MS.gene28384:intron 55.0%
TGCTGCAGCTGGAATTCCAG+GGG - chr4.4:9280622-9280641 MS.gene28384:intron 55.0%
TGGAATTCCAGGGGAGCCAA+TGG - chr4.4:9280631-9280650 MS.gene28384:intron 55.0%
TTGTACTCTGCTGCTCCTGG+GGG - chr4.4:9281364-9281383 MS.gene28384:CDS 55.0%
! GCATATGGTGGTCTTGCACG+AGG - chr4.4:9280695-9280714 MS.gene28384:intron 55.0%
AACCAGCCTCCCCAAACCCA+AGG + chr4.4:9281310-9281329 None:intergenic 60.0%
CGCCCCTCATGATTAGGGTC+AGG + chr4.4:9281044-9281063 None:intergenic 60.0%
GAGCCAGTTGTTGCTGCAGC+TGG - chr4.4:9280611-9280630 MS.gene28384:intron 60.0%
TTCCTTGGGTTTGGGGAGGC+TGG - chr4.4:9281305-9281324 MS.gene28384:CDS 60.0%
! TGGGTTTGGGGAGGCTGGTT+TGG - chr4.4:9281310-9281329 MS.gene28384:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 9280231 9281785 9280231 ID=MS.gene28384
chr4.4 mRNA 9280231 9281785 9280231 ID=MS.gene28384.t1;Parent=MS.gene28384
chr4.4 exon 9281778 9281785 9281778 ID=MS.gene28384.t1.exon1;Parent=MS.gene28384.t1
chr4.4 CDS 9281778 9281785 9281778 ID=cds.MS.gene28384.t1;Parent=MS.gene28384.t1
chr4.4 exon 9281130 9281427 9281130 ID=MS.gene28384.t1.exon2;Parent=MS.gene28384.t1
chr4.4 CDS 9281130 9281427 9281130 ID=cds.MS.gene28384.t1;Parent=MS.gene28384.t1
chr4.4 exon 9280375 9280440 9280375 ID=MS.gene28384.t1.exon3;Parent=MS.gene28384.t1
chr4.4 CDS 9280375 9280440 9280375 ID=cds.MS.gene28384.t1;Parent=MS.gene28384.t1
chr4.4 exon 9280231 9280290 9280231 ID=MS.gene28384.t1.exon4;Parent=MS.gene28384.t1
chr4.4 CDS 9280231 9280290 9280231 ID=cds.MS.gene28384.t1;Parent=MS.gene28384.t1
Gene Sequence

>MS.gene28384

ATGTCTGGGTATGTCAATTTTTGCTTAATTTACCTGTTTGTGAGATTCTACTTGTTAATGGTGTATTCATTATCTTAATTTGCATTTTTTTGTTGAATCTATATAGTTTTATTTTCTTTCATGAACTGCGTTTGTAAACTTTCAAATGCATTATAGATTTGATTTTAATTTGTAAGTACTACTATTGAAATAGTTGGATCTGATAGCCAATAGCCCTCGGTAGTAACTATAAGAAGTTTAGAACACAGATAACAGTATAGTATTTATTTAATTTTCAAAACTATTTTTTGTGTCTTGAATGGGTTTTAAGATATTATTTGATGATTTGCTGACTGTTATGAAACATTTTCTTCTTCAGAGAAGAGGAAGTTGTTGTAGCTGAGCCAGTTGTTGCTGCAGCTGGAATTCCAGGGGAGCCAATGGACATTATGACCGCTGTCCAGCTTGTATTGAGAAAGTCGCGGGCATATGGTGGTCTTGCACGAGGTCTTCATGAAAGTGCTAAAGTGATTGAGAAGCATGCTGCACAGCTCTGTGTTCTTGCTGAGGATTGTGACCAACCTGATTACGTCAAGTTGGTGAAAGCCCTTTGTGCTGAACACAGTGTCAGTTTGTTGACTGTTCCAAATGCAAAGACCCTTGGAGAATGGGCTGGTGTAAGTGCCCTTCCATTACTATAAACCTAGATTAATCCATTTCCTTGTGACATCCATTTGACCACAATTCTCAATTATATATTTTTTGATAAATTCATGAATTACTTTAAAATAAGTGTGGGAGTAACATCTCCCTCAACATCATGGTGCAAATCCTGACCCTAATCATGAGGGGCGCCAATAAGGAATTAATGAAACCTCTTGTGACCAACCTTTTGTTGTCTAAGAAATCCAACAAATAATTGCTTCTCTATAGATGTGACTTACTTAAACCTCCTAACTGTGATGCATGAAATGATAAATCTTTCTCATCAGACTCCTCCAATCCATAGAAATAGGGTACAACCCTTGAATGTCAAGCGGTGGACAGTTCTCAAATTTAGATTATGAATGTTGTTTAGTTATTGTTTCTGGTGTATTCCTTGGGTTTGGGGAGGCTGGTTTGGCAGCTTTTACTGTATTTGCCTTTGTTTTATCTTGTACTCTGCTGCTCCTGGGGGTGATTTTGTTTTTCTTTGTGATGTGTTTCTGTTTAGAGTACCCTACTCTGTATCCTCTCTGAACTCTGTAACCTCATTTATTTTAATCTTTGCCTTAAATTATTAAGAAGTTATTGTCTTGGTCTTTTTTTTATGCTTTCAAAATCTTGTCATTCTTTTGTACTGATTATGTGACGCACCTTCATGCAGTTGTGCAAAATTGATTCAGAAGGAAAAGCTAGGAAGGTAACTGGTTGCTCCTGTGTGGTTGTCAAGGTATGTTTTTCATCTGGAATAGAAATGTTTGGATTCTGTTTTCATCCTCTGTTTACGTATGCTTAACTATTTTCATTTTTGTAGGATTTCGGCGAGGAACATGAAGCATACAACGTTGTTCTTCAGCACGTGAAATCTAACTGA

Protein sequence

>MS.gene28384.t1

MSGEEEVVVAEPVVAAAGIPGEPMDIMTAVQLVLRKSRAYGGLARGLHESAKVIEKHAAQLCVLAEDCDQPDYVKLVKALCAEHSVSLLTVPNAKTLGEWAGLCKIDSEGKARKVTGCSCVVVKDFGEEHEAYNVVLQHVKSN