Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09762.t1 | XP_003611335.1 | 99.3 | 142 | 1 | 0 | 1 | 142 | 1 | 142 | 9.30E-73 | 282.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09762.t1 | Q9SKZ3 | 70.8 | 144 | 40 | 1 | 1 | 142 | 1 | 144 | 7.0e-53 | 208.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09762.t1 | G7JXD6 | 99.3 | 142 | 1 | 0 | 1 | 142 | 1 | 142 | 6.7e-73 | 282.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene09762 | 0.813457 | 2.54E-51 | -1.69E-46 |
| MS.gene051984 | MS.gene09762 | 0.807578 | 4.72E-50 | -1.69E-46 |
| MS.gene052664 | MS.gene09762 | 0.806107 | 9.66E-50 | -1.69E-46 |
| MS.gene053224 | MS.gene09762 | 0.843415 | 1.42E-58 | -1.69E-46 |
| MS.gene057643 | MS.gene09762 | 0.814983 | 1.17E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene03201 | MS.gene09762 | PPI |
| MS.gene09762 | MS.gene09779 | PPI |
| MS.gene09762 | MS.gene65180 | PPI |
| MS.gene09762 | MS.gene053443 | PPI |
| MS.gene09762 | MS.gene034249 | PPI |
| MS.gene02807 | MS.gene09762 | PPI |
| MS.gene57391 | MS.gene09762 | PPI |
| MS.gene09762 | MS.gene09780 | PPI |
| MS.gene09780 | MS.gene09762 | PPI |
| MS.gene09762 | MS.gene015411 | PPI |
| MS.gene013786 | MS.gene09762 | PPI |
| MS.gene09762 | MS.gene073888 | PPI |
| MS.gene09762 | MS.gene02572 | PPI |
| MS.gene030614 | MS.gene09762 | PPI |
| MS.gene09762 | MS.gene053224 | PPI |
| MS.gene09762 | MS.gene70215 | PPI |
| MS.gene09762 | MS.gene000610 | PPI |
| MS.gene09762 | MS.gene46264 | PPI |
| MS.gene09762 | MS.gene28384 | PPI |
| MS.gene53517 | MS.gene09762 | PPI |
| MS.gene09762 | MS.gene025830 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09762.t1 | MTR_5g012890 | 99.296 | 142 | 1 | 0 | 1 | 142 | 1 | 142 | 3.09e-101 | 286 |
| MS.gene09762.t1 | MTR_8g093770 | 94.444 | 126 | 7 | 0 | 17 | 142 | 18 | 143 | 2.31e-86 | 248 |
| MS.gene09762.t1 | MTR_4g006830 | 94.444 | 126 | 7 | 0 | 17 | 142 | 19 | 144 | 3.11e-85 | 246 |
| MS.gene09762.t1 | MTR_5g012890 | 96.262 | 107 | 4 | 0 | 1 | 107 | 1 | 107 | 5.85e-71 | 208 |
| MS.gene09762.t1 | MTR_4g073510 | 70.370 | 54 | 14 | 1 | 1 | 52 | 1 | 54 | 6.42e-17 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09762.t1 | AT2G32060 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 1.33e-66 | 199 |
| MS.gene09762.t1 | AT2G32060 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 1.33e-66 | 199 |
| MS.gene09762.t1 | AT2G32060 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 1.33e-66 | 199 |
| MS.gene09762.t1 | AT1G15930 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 5.31e-64 | 192 |
| MS.gene09762.t1 | AT1G15930 | 74.400 | 125 | 31 | 1 | 18 | 142 | 21 | 144 | 5.31e-64 | 192 |
Find 35 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACCAATTTGACATAATC+AGG | 0.268051 | 5.1:-6028274 | None:intergenic |
| GTTGTTGCTGCTGCAATTCC+AGG | 0.269519 | 5.1:+6028103 | MS.gene09762:CDS |
| GATGCTCTTGTGTGGTTGTC+AGG | 0.354575 | 5.1:+6028593 | MS.gene09762:CDS |
| ATGCTGCAAAACAACATTGT+AGG | 0.363171 | 5.1:-6028729 | None:intergenic |
| CATAAGCACGAGACTTTCTA+AGG | 0.363450 | 5.1:-6028162 | None:intergenic |
| CCAACCTGATTATGTCAAAT+TGG | 0.373462 | 5.1:+6028270 | MS.gene09762:CDS |
| CTTAGAAAGTCTCGTGCTTA+TGG | 0.383982 | 5.1:+6028163 | MS.gene09762:CDS |
| ATCAGGTTGGTCACAATCTT+CGG | 0.412966 | 5.1:-6028257 | None:intergenic |
| ATGATGTCCATTGGCTCGCC+TGG | 0.437864 | 5.1:-6028121 | None:intergenic |
| TCTCCAAGGGTCTTTGCACT+TGG | 0.459795 | 5.1:-6028337 | None:intergenic |
| TATCAACCAAGAAATATGTC+AGG | 0.459989 | 5.1:+6027228 | None:intergenic |
| GGAATTGCAGCAGCAACAAC+AGG | 0.463311 | 5.1:-6028100 | None:intergenic |
| GGTAAAGCTAGGAAGGTTAC+CGG | 0.476827 | 5.1:+6028571 | MS.gene09762:CDS |
| AACATTGTGCTCTGCGCAGA+GGG | 0.503587 | 5.1:-6028299 | None:intergenic |
| GACAACCACACAAGAGCATC+CGG | 0.522597 | 5.1:-6028590 | None:intergenic |
| TTACGCCAGCCCACTCTCCA+AGG | 0.529322 | 5.1:-6028351 | None:intergenic |
| CCAATTTGACATAATCAGGT+TGG | 0.530247 | 5.1:-6028270 | None:intergenic |
| TGCAAAGACCCTTGGAGAGT+GGG | 0.530356 | 5.1:+6028342 | MS.gene09762:CDS |
| TTTGTTATGCAGTGAAGAAG+AGG | 0.537025 | 5.1:+6028063 | MS.gene09762:intron |
| GTTCCAAGTGCAAAGACCCT+TGG | 0.537039 | 5.1:+6028334 | MS.gene09762:CDS |
| AAGACCCTTGGAGAGTGGGC+TGG | 0.538691 | 5.1:+6028346 | MS.gene09762:CDS |
| TTGATTCAGAAGGTAAAGCT+AGG | 0.543764 | 5.1:+6028560 | MS.gene09762:CDS |
| TTCAGAAGGTAAAGCTAGGA+AGG | 0.557714 | 5.1:+6028564 | MS.gene09762:CDS |
| TGCAATTCCAGGCGAGCCAA+TGG | 0.568250 | 5.1:+6028114 | MS.gene09762:CDS |
| GTGCAAAGACCCTTGGAGAG+TGG | 0.569975 | 5.1:+6028341 | MS.gene09762:CDS |
| ATGCTCTTGTGTGGTTGTCA+GGG | 0.586706 | 5.1:+6028594 | MS.gene09762:CDS |
| AAAGCAGTCATGATGTCCAT+TGG | 0.589394 | 5.1:-6028130 | None:intergenic |
| CTTGCACGAGGTCTTCATGA+AGG | 0.605005 | 5.1:+6028190 | MS.gene09762:CDS |
| AGAAAGTCTCGTGCTTATGG+TGG | 0.620799 | 5.1:+6028166 | MS.gene09762:CDS |
| TTGTGCAAGATTGATTCAGA+AGG | 0.623879 | 5.1:+6028550 | MS.gene09762:CDS |
| TACGCCAGCCCACTCTCCAA+GGG | 0.624257 | 5.1:-6028350 | None:intergenic |
| GCTTATGGTGGTCTTGCACG+AGG | 0.624672 | 5.1:+6028178 | MS.gene09762:CDS |
| TAACATTGTGCTCTGCGCAG+AGG | 0.632440 | 5.1:-6028300 | None:intergenic |
| ATCAACCAAGAAATATGTCA+GGG | 0.636797 | 5.1:+6027229 | None:intergenic |
| GGTTACCGGATGCTCTTGTG+TGG | 0.638821 | 5.1:+6028585 | MS.gene09762:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGTTCACAATATAAATT+TGG | - | chr5.1:6028440-6028459 | None:intergenic | 15.0% |
| !! | AAAGTTCACAATATAAATTT+GGG | - | chr5.1:6028439-6028458 | None:intergenic | 15.0% |
| !!! | ATTCTTCTATTTATATGTTT+TGG | + | chr5.1:6027964-6027983 | MS.gene09762:intron | 15.0% |
| !! | AAAATAAGATAAATCCAACT+AGG | + | chr5.1:6027740-6027759 | MS.gene09762:intron | 20.0% |
| !! | ACAATATAAATTTGGGAATT+GGG | - | chr5.1:6028432-6028451 | None:intergenic | 20.0% |
| !! | ATTACTTGTAGTTATTACTA+AGG | - | chr5.1:6027852-6027871 | None:intergenic | 20.0% |
| !! | TTCAAATGCAATTGTAATTT+AGG | + | chr5.1:6027295-6027314 | MS.gene09762:intron | 20.0% |
| !!! | TAAAGATTTTTAAACTTGCA+AGG | - | chr5.1:6028017-6028036 | None:intergenic | 20.0% |
| ! | AAAACTAGGTTAAGAATCAA+GGG | - | chr5.1:6028380-6028399 | None:intergenic | 25.0% |
| ! | AACTTATGAAAACAGCTTAT+GGG | - | chr5.1:6027413-6027432 | None:intergenic | 25.0% |
| ! | ATCCTGCAAACAAAAAAAAA+AGG | - | chr5.1:6028690-6028709 | None:intergenic | 25.0% |
| ! | ATCTTAAACGATAAAACTAC+GGG | - | chr5.1:6027766-6027785 | None:intergenic | 25.0% |
| ! | CACAATATAAATTTGGGAAT+TGG | - | chr5.1:6028433-6028452 | None:intergenic | 25.0% |
| ! | GATCTTAAACGATAAAACTA+CGG | - | chr5.1:6027767-6027786 | None:intergenic | 25.0% |
| ! | TAAAACTAGGTTAAGAATCA+AGG | - | chr5.1:6028381-6028400 | None:intergenic | 25.0% |
| ! | TCCTGCAAACAAAAAAAAAA+GGG | - | chr5.1:6028689-6028708 | None:intergenic | 25.0% |
| ! | TTAGCATGAAAAAAGACAAT+AGG | - | chr5.1:6028648-6028667 | None:intergenic | 25.0% |
| !! | AATGTATAGAACTTTTTACC+AGG | - | chr5.1:6027947-6027966 | None:intergenic | 25.0% |
| !!! | CTTTATTTTTTCTTTTGCTG+TGG | - | chr5.1:6027371-6027390 | None:intergenic | 25.0% |
| !!! | TTTTTTGTTTGCAGGATTTT+GGG | + | chr5.1:6028693-6028712 | MS.gene09762:intron | 25.0% |
| !!! | TTTTTTTGTTTGCAGGATTT+TGG | + | chr5.1:6028692-6028711 | MS.gene09762:intron | 25.0% |
| ACAAGGAATTGGATAAAACT+AGG | - | chr5.1:6028394-6028413 | None:intergenic | 30.0% | |
| ACCACTAGATAAGTTCAATT+AGG | + | chr5.1:6027531-6027550 | MS.gene09762:intron | 30.0% | |
| AGATAACTGAATCTGATATC+CGG | + | chr5.1:6027823-6027842 | MS.gene09762:intron | 30.0% | |
| CAACTTATGAAAACAGCTTA+TGG | - | chr5.1:6027414-6027433 | None:intergenic | 30.0% | |
| TGGAAATGAGTTATGATAAG+TGG | - | chr5.1:6027351-6027370 | None:intergenic | 30.0% | |
| TTCACCAATTTGACATAATC+AGG | - | chr5.1:6028277-6028296 | None:intergenic | 30.0% | |
| !! | GCTGTTTTCATAAGTTGTAT+TGG | + | chr5.1:6027417-6027436 | MS.gene09762:intron | 30.0% |
| !! | TTTTTGTTTGCAGGATTTTG+GGG | + | chr5.1:6028694-6028713 | MS.gene09762:intron | 30.0% |
| !!! | TCCCTTTTTTTTTTGTTTGC+AGG | + | chr5.1:6028685-6028704 | MS.gene09762:intron | 30.0% |
| AAATAAGCTGAAGACAGCTA+AGG | + | chr5.1:6027451-6027470 | MS.gene09762:intron | 35.0% | |
| ACAAATAAGCCAATCCAAAC+AGG | - | chr5.1:6027564-6027583 | None:intergenic | 35.0% | |
| AGATAAGTTCAATTAGGCCA+AGG | + | chr5.1:6027537-6027556 | MS.gene09762:intron | 35.0% | |
| ATCAATCTTGCACAACTACA+TGG | - | chr5.1:6028545-6028564 | None:intergenic | 35.0% | |
| ATCAGATGTTTGTCCAAACA+GGG | - | chr5.1:6027321-6027340 | None:intergenic | 35.0% | |
| ATGCTGCAAAACAACATTGT+AGG | - | chr5.1:6028732-6028751 | None:intergenic | 35.0% | |
| ATTGTAATTTAGGCCCTGTT+TGG | + | chr5.1:6027305-6027324 | MS.gene09762:intron | 35.0% | |
| CCAACCTGATTATGTCAAAT+TGG | + | chr5.1:6028270-6028289 | MS.gene09762:CDS | 35.0% | |
| CCAATTTGACATAATCAGGT+TGG | - | chr5.1:6028273-6028292 | None:intergenic | 35.0% | |
| GATAAGTTCAATTAGGCCAA+GGG | + | chr5.1:6027538-6027557 | MS.gene09762:intron | 35.0% | |
| GCCTAATTGAACTTATCTAG+TGG | - | chr5.1:6027535-6027554 | None:intergenic | 35.0% | |
| GTAGTTATTACTAAGGTGAC+CGG | - | chr5.1:6027845-6027864 | None:intergenic | 35.0% | |
| TATCAGATGTTTGTCCAAAC+AGG | - | chr5.1:6027322-6027341 | None:intergenic | 35.0% | |
| TTGATTCAGAAGGTAAAGCT+AGG | + | chr5.1:6028560-6028579 | MS.gene09762:CDS | 35.0% | |
| ! | TTGTGCAAGATTGATTCAGA+AGG | + | chr5.1:6028550-6028569 | MS.gene09762:CDS | 35.0% |
| ! | TTTGTTATGCAGTGAAGAAG+AGG | + | chr5.1:6028063-6028082 | MS.gene09762:intron | 35.0% |
| !! | AAACTGATTCGAGTTGTTTC+AGG | - | chr5.1:6027794-6027813 | None:intergenic | 35.0% |
| AAAGCAGTCATGATGTCCAT+TGG | - | chr5.1:6028133-6028152 | None:intergenic | 40.0% | |
| AATGGATGTGCACAAGGAAT+TGG | - | chr5.1:6028405-6028424 | None:intergenic | 40.0% | |
| AGCTATACATGAGACACACA+AGG | - | chr5.1:6027991-6028010 | None:intergenic | 40.0% | |
| ATTTGGGAATTGGGAGCAAA+TGG | - | chr5.1:6028423-6028442 | None:intergenic | 40.0% | |
| CATAAGCACGAGACTTTCTA+AGG | - | chr5.1:6028165-6028184 | None:intergenic | 40.0% | |
| CTTAGAAAGTCTCGTGCTTA+TGG | + | chr5.1:6028163-6028182 | MS.gene09762:CDS | 40.0% | |
| TGTCTCAAGATTTATGCTCC+TGG | + | chr5.1:6027926-6027945 | MS.gene09762:intron | 40.0% | |
| TTCAGAAGGTAAAGCTAGGA+AGG | + | chr5.1:6028564-6028583 | MS.gene09762:CDS | 40.0% | |
| !! | ATCAGGTTGGTCACAATCTT+CGG | - | chr5.1:6028260-6028279 | None:intergenic | 40.0% |
| AATGATGCAACAAGACTGCG+CGG | - | chr5.1:6028489-6028508 | None:intergenic | 45.0% | |
| AGAAAGTCTCGTGCTTATGG+TGG | + | chr5.1:6028166-6028185 | MS.gene09762:CDS | 45.0% | |
| ATGCTCTTGTGTGGTTGTCA+GGG | + | chr5.1:6028594-6028613 | MS.gene09762:CDS | 45.0% | |
| GATAAAACTACGGGCCTAGT+TGG | - | chr5.1:6027757-6027776 | None:intergenic | 45.0% | |
| GGTAAAGCTAGGAAGGTTAC+CGG | + | chr5.1:6028571-6028590 | MS.gene09762:CDS | 45.0% | |
| !! | TTGTTTGCAGGATTTTGGGG+AGG | + | chr5.1:6028697-6028716 | MS.gene09762:intron | 45.0% |
| !!! | TATTTATTTTATTGATTTTT+GGG | + | chr5.1:6028510-6028529 | MS.gene09762:intron | 5.0% |
| !!! | TTATTTATTTTATTGATTTT+TGG | + | chr5.1:6028509-6028528 | MS.gene09762:intron | 5.0% |
| AACATTGTGCTCTGCGCAGA+GGG | - | chr5.1:6028302-6028321 | None:intergenic | 50.0% | |
| AATTAGGCCAAGGGCCTGTT+TGG | + | chr5.1:6027547-6027566 | MS.gene09762:intron | 50.0% | |
| ATGATGCAACAAGACTGCGC+GGG | - | chr5.1:6028488-6028507 | None:intergenic | 50.0% | |
| CTTGCACGAGGTCTTCATGA+AGG | + | chr5.1:6028190-6028209 | MS.gene09762:CDS | 50.0% | |
| CTTGTGTGGTTGTCAGGGTA+TGG | + | chr5.1:6028599-6028618 | MS.gene09762:intron | 50.0% | |
| GATGCTCTTGTGTGGTTGTC+AGG | + | chr5.1:6028593-6028612 | MS.gene09762:CDS | 50.0% | |
| GGAATTGCAGCAGCAACAAC+AGG | - | chr5.1:6028103-6028122 | None:intergenic | 50.0% | |
| GGAGCAAATGGATGTGCACA+AGG | - | chr5.1:6028411-6028430 | None:intergenic | 50.0% | |
| GTTCCAAGTGCAAAGACCCT+TGG | + | chr5.1:6028334-6028353 | MS.gene09762:CDS | 50.0% | |
| GTTGTTGCTGCTGCAATTCC+AGG | + | chr5.1:6028103-6028122 | MS.gene09762:CDS | 50.0% | |
| TAACATTGTGCTCTGCGCAG+AGG | - | chr5.1:6028303-6028322 | None:intergenic | 50.0% | |
| TCTCCAAGGGTCTTTGCACT+TGG | - | chr5.1:6028340-6028359 | None:intergenic | 50.0% | |
| TGCAAAGACCCTTGGAGAGT+GGG | + | chr5.1:6028342-6028361 | MS.gene09762:CDS | 50.0% | |
| !! | GACAACCACACAAGAGCATC+CGG | - | chr5.1:6028593-6028612 | None:intergenic | 50.0% |
| AGCCAATCCAAACAGGCCCT+TGG | - | chr5.1:6027557-6027576 | None:intergenic | 55.0% | |
| GGTTACCGGATGCTCTTGTG+TGG | + | chr5.1:6028585-6028604 | MS.gene09762:CDS | 55.0% | |
| GTGCAAAGACCCTTGGAGAG+TGG | + | chr5.1:6028341-6028360 | MS.gene09762:CDS | 55.0% | |
| TGCAATTCCAGGCGAGCCAA+TGG | + | chr5.1:6028114-6028133 | MS.gene09762:CDS | 55.0% | |
| ! | GCTTATGGTGGTCTTGCACG+AGG | + | chr5.1:6028178-6028197 | MS.gene09762:CDS | 55.0% |
| !! | ATGATGTCCATTGGCTCGCC+TGG | - | chr5.1:6028124-6028143 | None:intergenic | 55.0% |
| AAGACCCTTGGAGAGTGGGC+TGG | + | chr5.1:6028346-6028365 | MS.gene09762:CDS | 60.0% | |
| GGCCAAGGGCCTGTTTGGAT+TGG | + | chr5.1:6027552-6027571 | MS.gene09762:intron | 60.0% | |
| TACGCCAGCCCACTCTCCAA+GGG | - | chr5.1:6028353-6028372 | None:intergenic | 60.0% | |
| TTACGCCAGCCCACTCTCCA+AGG | - | chr5.1:6028354-6028373 | None:intergenic | 60.0% | |
| GCAACAAGACTGCGCGGGCA+TGG | - | chr5.1:6028483-6028502 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 6027243 | 6028766 | 6027243 | ID=MS.gene09762 |
| chr5.1 | mRNA | 6027243 | 6028766 | 6027243 | ID=MS.gene09762.t1;Parent=MS.gene09762 |
| chr5.1 | exon | 6027243 | 6027250 | 6027243 | ID=MS.gene09762.t1.exon1;Parent=MS.gene09762.t1 |
| chr5.1 | CDS | 6027243 | 6027250 | 6027243 | ID=cds.MS.gene09762.t1;Parent=MS.gene09762.t1 |
| chr5.1 | exon | 6028075 | 6028369 | 6028075 | ID=MS.gene09762.t1.exon2;Parent=MS.gene09762.t1 |
| chr5.1 | CDS | 6028075 | 6028369 | 6028075 | ID=cds.MS.gene09762.t1;Parent=MS.gene09762.t1 |
| chr5.1 | exon | 6028550 | 6028615 | 6028550 | ID=MS.gene09762.t1.exon3;Parent=MS.gene09762.t1 |
| chr5.1 | CDS | 6028550 | 6028615 | 6028550 | ID=cds.MS.gene09762.t1;Parent=MS.gene09762.t1 |
| chr5.1 | exon | 6028707 | 6028766 | 6028707 | ID=MS.gene09762.t1.exon4;Parent=MS.gene09762.t1 |
| chr5.1 | CDS | 6028707 | 6028766 | 6028707 | ID=cds.MS.gene09762.t1;Parent=MS.gene09762.t1 |
| Gene Sequence |
| Protein sequence |
