AlfalfaGEDB | MS.gene65180

M. sativa cultivar XinJiangDaYe / MS.gene65180

KEGG: K02951
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01248.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65180.t1	XP_003611335.1	94.4	142	2	1	1	136	1	142	1.20E-66	262.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65180.t1	Q9XHS0	79.0	124	26	0	12	135	19	142	9.7e-52	204.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65180.t1	G7JXD6	94.4	142	2	1	1	136	1	142	8.9e-67	262.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene09780	MS.gene65180	PPI
MS.gene46264	MS.gene65180	PPI
MS.gene65180	MS.gene09779	PPI
MS.gene030614	MS.gene65180	PPI
MS.gene09762	MS.gene65180	PPI
MS.gene65180	MS.gene025830	PPI
MS.gene65180	MS.gene053443	PPI
MS.gene03201	MS.gene65180	PPI
MS.gene70215	MS.gene65180	PPI
MS.gene65180	MS.gene034249	PPI
MS.gene53517	MS.gene65180	PPI
MS.gene28384	MS.gene65180	PPI
MS.gene65180	MS.gene015411	PPI
MS.gene65180	MS.gene073888	PPI
MS.gene65180	MS.gene02572	PPI
MS.gene65180	MS.gene053224	PPI
MS.gene013786	MS.gene65180	PPI
MS.gene65180	MS.gene000610	PPI
MS.gene02807	MS.gene65180	PPI
MS.gene57391	MS.gene65180	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65180.t1	MTR_5g012890	99.200	125	1	0	12	136	18	142	1.42e-88	254
MS.gene65180.t1	MTR_8g093770	95.200	125	6	0	12	136	19	143	1.39e-85	246
MS.gene65180.t1	MTR_4g006830	94.444	126	7	0	11	136	19	144	2.79e-84	243
MS.gene65180.t1	MTR_5g012890	94.444	90	5	0	12	101	18	107	3.02e-58	176
MS.gene65180.t1	MTR_4g073510	62.264	53	12	1	1	45	1	53	1.12e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65180.t1	AT2G32060	74.400	125	31	1	12	136	21	144	1.31e-65	196
MS.gene65180.t1	AT2G32060	74.400	125	31	1	12	136	21	144	1.31e-65	196
MS.gene65180.t1	AT2G32060	74.400	125	31	1	12	136	21	144	1.31e-65	196
MS.gene65180.t1	AT1G15930	74.400	125	31	1	12	136	21	144	5.38e-63	189
MS.gene65180.t1	AT1G15930	74.400	125	31	1	12	136	21	144	5.38e-63	189

Find 32 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCTGCAAAACAACATTAT+AGG	0.217837	7.1:+41320467	None:intergenic
TTCACCAATTTGACATAATC+AGG	0.268051	7.1:+41320651	None:intergenic
TGTGTAGTTGTCAAGGATTT+TGG	0.355852	7.1:-41320504	MS.gene65180:CDS
CCAACCTGATTATGTCAAAT+TGG	0.373462	7.1:-41320655	MS.gene65180:CDS
GTTGTTGCAGTTGCAATTCC+AGG	0.397742	7.1:-41320822	MS.gene65180:CDS
CTTAGAAAGTCTCGTGCTTA+TGG	0.404639	7.1:-41320762	MS.gene65180:CDS
ACTTTCTAAGCACAAGTTGT+AGG	0.404720	7.1:+41320775	None:intergenic
TATCAACCAAGAAATATGTT+TGG	0.406417	7.1:-41320855	None:intergenic
ATCAGGTTGGTCACAATCTT+CGG	0.412966	7.1:+41320668	None:intergenic
ATGATGTCCATTGGCTCACC+TGG	0.429738	7.1:+41320804	None:intergenic
GGTAAAGCTAGGAAGGTTAC+CGG	0.476827	7.1:-41320534	MS.gene65180:CDS
AACATTGTGCTCTGCGCAGA+GGG	0.503587	7.1:+41320626	None:intergenic
CTTTCTAAGCACAAGTTGTA+GGG	0.509554	7.1:+41320776	None:intergenic
GACAACTACACAAGAGCATC+CGG	0.520115	7.1:+41320515	None:intergenic
CCAATTTGACATAATCAGGT+TGG	0.530247	7.1:+41320655	None:intergenic
TGCAAAGACCCTTGGAGAGT+GGG	0.530356	7.1:-41320583	MS.gene65180:CDS
AAGACCCTTGGAGAGTGGGC+TGG	0.532172	7.1:-41320579	MS.gene65180:CDS
GTTGCAAGTGCAAAGACCCT+TGG	0.543554	7.1:-41320591	MS.gene65180:CDS
TTGATTCAGAAGGTAAAGCT+AGG	0.543764	7.1:-41320545	MS.gene65180:CDS
AAATATGTTTGGTGAAGAAG+AGG	0.545898	7.1:-41320844	None:intergenic
TGCAATTCCAGGTGAGCCAA+TGG	0.546807	7.1:-41320811	MS.gene65180:CDS
AGGGCAGTCATGATGTCCAT+TGG	0.551573	7.1:+41320795	None:intergenic
TTCAGAAGGTAAAGCTAGGA+AGG	0.557714	7.1:-41320541	MS.gene65180:CDS
ATGCTCTTGTGTAGTTGTCA+AGG	0.563450	7.1:-41320511	MS.gene65180:CDS
ACAAACCAGCCCACTCTCCA+AGG	0.573283	7.1:+41320574	None:intergenic
GTGCAAAGACCCTTGGAGAG+TGG	0.578134	7.1:-41320584	MS.gene65180:CDS
TTGTGCAAGATTGATTCAGA+AGG	0.594862	7.1:-41320555	MS.gene65180:CDS
CTTGCACGAGGTCTTCATGA+AGG	0.605005	7.1:-41320735	MS.gene65180:CDS
AGAAAGTCTCGTGCTTATGG+TGG	0.620799	7.1:-41320759	MS.gene65180:CDS
GCTTATGGTGGTCTTGCACG+AGG	0.624672	7.1:-41320747	MS.gene65180:CDS
CAAACCAGCCCACTCTCCAA+GGG	0.627221	7.1:+41320575	None:intergenic
TAACATTGTGCTCTGCGCAG+AGG	0.632440	7.1:+41320625	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGCTGCAAAACAACATTAT+AGG	+	chr7.1:41320828-41320847	None:intergenic	30.0%
	TTCACCAATTTGACATAATC+AGG	+	chr7.1:41320644-41320663	None:intergenic	30.0%
	ACTTTCTAAGCACAAGTTGT+AGG	+	chr7.1:41320520-41320539	None:intergenic	35.0%
	CCAACCTGATTATGTCAAAT+TGG	-	chr7.1:41320637-41320656	MS.gene65180:CDS	35.0%
	CCAATTTGACATAATCAGGT+TGG	+	chr7.1:41320640-41320659	None:intergenic	35.0%
	CTTTCTAAGCACAAGTTGTA+GGG	+	chr7.1:41320519-41320538	None:intergenic	35.0%
	TTGATTCAGAAGGTAAAGCT+AGG	-	chr7.1:41320747-41320766	MS.gene65180:CDS	35.0%
!	TGTGTAGTTGTCAAGGATTT+TGG	-	chr7.1:41320788-41320807	MS.gene65180:CDS	35.0%
!	TTGTGCAAGATTGATTCAGA+AGG	-	chr7.1:41320737-41320756	MS.gene65180:CDS	35.0%
	ATGCTCTTGTGTAGTTGTCA+AGG	-	chr7.1:41320781-41320800	MS.gene65180:CDS	40.0%
	CTTAGAAAGTCTCGTGCTTA+TGG	-	chr7.1:41320530-41320549	MS.gene65180:CDS	40.0%
	TTCAGAAGGTAAAGCTAGGA+AGG	-	chr7.1:41320751-41320770	MS.gene65180:CDS	40.0%
!!	AGTTGTCAAGGATTTTGGTG+AGG	-	chr7.1:41320793-41320812	MS.gene65180:CDS	40.0%
!!	ATCAGGTTGGTCACAATCTT+CGG	+	chr7.1:41320627-41320646	None:intergenic	40.0%
	AGAAAGTCTCGTGCTTATGG+TGG	-	chr7.1:41320533-41320552	MS.gene65180:CDS	45.0%
	GGTAAAGCTAGGAAGGTTAC+CGG	-	chr7.1:41320758-41320777	MS.gene65180:CDS	45.0%
	GTTGTTGCAGTTGCAATTCC+AGG	-	chr7.1:41320470-41320489	MS.gene65180:CDS	45.0%
!!	GACAACTACACAAGAGCATC+CGG	+	chr7.1:41320780-41320799	None:intergenic	45.0%
	AACATTGTGCTCTGCGCAGA+GGG	+	chr7.1:41320669-41320688	None:intergenic	50.0%
	AGGGCAGTCATGATGTCCAT+TGG	+	chr7.1:41320500-41320519	None:intergenic	50.0%
	CTTGCACGAGGTCTTCATGA+AGG	-	chr7.1:41320557-41320576	MS.gene65180:CDS	50.0%
	GTTGCAAGTGCAAAGACCCT+TGG	-	chr7.1:41320701-41320720	MS.gene65180:CDS	50.0%
	TAACATTGTGCTCTGCGCAG+AGG	+	chr7.1:41320670-41320689	None:intergenic	50.0%
	TGCAAAGACCCTTGGAGAGT+GGG	-	chr7.1:41320709-41320728	MS.gene65180:CDS	50.0%
	TGCAATTCCAGGTGAGCCAA+TGG	-	chr7.1:41320481-41320500	MS.gene65180:CDS	50.0%
!!	ATGATGTCCATTGGCTCACC+TGG	+	chr7.1:41320491-41320510	None:intergenic	50.0%
	ACAAACCAGCCCACTCTCCA+AGG	+	chr7.1:41320721-41320740	None:intergenic	55.0%
	CAAACCAGCCCACTCTCCAA+GGG	+	chr7.1:41320720-41320739	None:intergenic	55.0%
	GTGCAAAGACCCTTGGAGAG+TGG	-	chr7.1:41320708-41320727	MS.gene65180:CDS	55.0%
!	GCTTATGGTGGTCTTGCACG+AGG	-	chr7.1:41320545-41320564	MS.gene65180:CDS	55.0%
	AAGACCCTTGGAGAGTGGGC+TGG	-	chr7.1:41320713-41320732	MS.gene65180:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	41320452	41320862	41320452	ID=MS.gene65180
chr7.1	mRNA	41320452	41320862	41320452	ID=MS.gene65180.t1;Parent=MS.gene65180
chr7.1	exon	41320452	41320862	41320452	ID=MS.gene65180.t1.exon1;Parent=MS.gene65180.t1
chr7.1	CDS	41320452	41320862	41320452	ID=cds.MS.gene65180.t1;Parent=MS.gene65180.t1

Gene Sequence

>MS.gene65180

ATGTTTGGTGAAGAAGAGGTTGTTGCAGTTGCAATTCCAGGTGAGCCAATGGACATCATGACTGCCCTACAACTTGTGCTTAGAAAGTCTCGTGCTTATGGTGGTCTTGCACGAGGTCTTCATGAAGGTGCAAAAGTGATTGAGAAGCATGCTGCACAGCTTTGTGTTCTAGCCGAAGATTGTGACCAACCTGATTATGTCAAATTGGTGAAAGCCCTCTGCGCAGAGCACAATGTTAGCTTGTTGACAGTTGCAAGTGCAAAGACCCTTGGAGAGTGGGCTGGTTTGTGCAAGATTGATTCAGAAGGTAAAGCTAGGAAGGTTACCGGATGCTCTTGTGTAGTTGTCAAGGATTTTGGTGAGGAACATGAAGCCTATAATGTTGTTTTGCAGCATGTAAAAGCTAACTAA

Protein sequence

>MS.gene65180.t1

MFGEEEVVAVAIPGEPMDIMTALQLVLRKSRAYGGLARGLHEGAKVIEKHAAQLCVLAEDCDQPDYVKLVKALCAEHNVSLLTVASAKTLGEWAGLCKIDSEGKARKVTGCSCVVVKDFGEEHEAYNVVLQHVKAN