AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57391


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57391.t1 MTR_8g093770 100.000 143 0 0 1 143 1 143 1.51e-102 290
MS.gene57391.t1 MTR_5g012890 96.000 125 5 0 19 143 18 142 1.01e-86 249
MS.gene57391.t1 MTR_4g006830 88.889 144 15 1 1 143 1 144 2.91e-83 241
MS.gene57391.t1 MTR_5g012890 91.111 90 8 0 19 108 18 107 1.38e-56 172
MS.gene57391.t1 MTR_4g073510 66.667 54 17 1 1 53 1 54 3.27e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57391.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene57391.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene57391.t1 AT2G32060 76.000 125 29 1 19 143 21 144 1.29e-67 201
MS.gene57391.t1 AT1G15930 74.400 125 31 1 19 143 21 144 3.49e-64 192
MS.gene57391.t1 AT1G15930 74.400 125 31 1 19 143 21 144 3.49e-64 192

Find 33 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCAACTTGACGTAATC+AGG 0.299059 4.2:-9046887 None:intergenic
TCTCCAAGGGTCTTTGCATT+TGG 0.313809 4.2:-9046950 None:intergenic
GTTGCTGCAGCTGGAATTCC+AGG 0.331115 4.2:+9046716 MS.gene57391:CDS
AATCAACTTGCAATTATGTC+TGG 0.359633 4.2:+9046098 None:intergenic
ATTCCAGCTGCAGCAACAAC+TGG 0.406602 4.2:-9046710 None:intergenic
TGCAAAGACCCTTGGAGAAT+GGG 0.412944 4.2:+9046955 MS.gene57391:CDS
ATAATATCCATTGGCTCCCC+TGG 0.419454 4.2:-9046734 None:intergenic
GAGCCAGTTGTTGCTGCAGC+TGG 0.425162 4.2:+9046707 MS.gene57391:CDS
TTGAGAAAGTCACGCGCTTA+TGG 0.428624 4.2:+9046776 MS.gene57391:CDS
GACACTGTGTTCAGCACAAA+GGG 0.447539 4.2:-9046912 None:intergenic
ATGCAAAGACCCTTGGAGAA+TGG 0.451113 4.2:+9046954 MS.gene57391:CDS
GGAAAAGCTAGGAAGGTAAC+TGG 0.463250 4.2:+9047660 MS.gene57391:CDS
TTGCTCCTGTGTGGTTGTCA+AGG 0.525394 4.2:+9047683 MS.gene57391:CDS
GTTCCAAATGCAAAGACCCT+TGG 0.532260 4.2:+9046947 MS.gene57391:CDS
TTGCTGCAGCTGGAATTCCA+GGG 0.537879 4.2:+9046717 MS.gene57391:CDS
ATCAACTTGCAATTATGTCT+GGG 0.539041 4.2:+9046099 None:intergenic
TTGTGCAAAATTGATTCAGA+AGG 0.565495 4.2:+9047639 MS.gene57391:CDS
ACTGCAGTCATAATATCCAT+TGG 0.568889 4.2:-9046743 None:intergenic
TGCTGCAGCTGGAATTCCAG+GGG 0.575795 4.2:+9046718 MS.gene57391:CDS
TTGATTCAGAAGGAAAAGCT+AGG 0.578774 4.2:+9047649 MS.gene57391:CDS
ACAGCTCTGTGTTCTTGCTG+AGG 0.587565 4.2:+9046853 MS.gene57391:CDS
CCAACCTGATTACGTCAAGT+TGG 0.591510 4.2:+9046883 MS.gene57391:CDS
TTACACCAGCCCATTCTCCA+AGG 0.592149 4.2:-9046964 None:intergenic
TTCAGAAGGAAAAGCTAGGA+AGG 0.596975 4.2:+9047653 MS.gene57391:CDS
TGGAATTCCAGGGGAGCCAA+TGG 0.602144 4.2:+9046727 MS.gene57391:CDS
AGAAAGTCACGCGCTTATGG+TGG 0.613205 4.2:+9046779 MS.gene57391:CDS
CCAACTTGACGTAATCAGGT+TGG 0.614249 4.2:-9046883 None:intergenic
AAGACCCTTGGAGAATGGGC+TGG 0.615483 4.2:+9046959 MS.gene57391:CDS
TGACACTGTGTTCAGCACAA+AGG 0.628583 4.2:-9046913 None:intergenic
GCTTATGGTGGTCTTGCACG+TGG 0.635715 4.2:+9046791 MS.gene57391:CDS
GGTAACTGGTTGCTCCTGTG+TGG 0.641819 4.2:+9047674 MS.gene57391:CDS
TACACCAGCCCATTCTCCAA+GGG 0.643748 4.2:-9046963 None:intergenic
ACATACCTTGACAACCACAC+AGG 0.682837 4.2:-9047688 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATAATTGAGAATTG+TGG - chr4.2:9047045-9047064 None:intergenic 15.0%
!! CATATAGATTAAAATAAATG+AGG - chr4.2:9047511-9047530 None:intergenic 15.0%
!! TATAAAATGTTATTGCTTAT+TGG - chr4.2:9047188-9047207 None:intergenic 15.0%
!!! AATTACTTTAAAATAAGTGT+GGG + chr4.2:9047080-9047099 MS.gene57391:intron 15.0%
!!! TTAACTATTTTCATTTTTGT+AGG + chr4.2:9047767-9047786 MS.gene57391:intron 15.0%
!!! TTATAATAACTTTTTGATCT+GGG - chr4.2:9046494-9046513 None:intergenic 15.0%
!!! TTTATAATAACTTTTTGATC+TGG - chr4.2:9046495-9046514 None:intergenic 15.0%
!! AATAGTTAAGCATACATAAA+CGG - chr4.2:9047756-9047775 None:intergenic 20.0%
!! ATTGATTATGAGGATAATTA+TGG + chr4.2:9046442-9046461 MS.gene57391:intron 20.0%
!! CAAAACCAATATAAAACAAA+AGG - chr4.2:9047421-9047440 None:intergenic 20.0%
!!! ATTTGCCTTTTGTTTTATAT+TGG + chr4.2:9047413-9047432 MS.gene57391:intron 20.0%
!!! GAATTACTTTAAAATAAGTG+TGG + chr4.2:9047079-9047098 MS.gene57391:intron 20.0%
!!! TTTTCATTTTTGTAGGATTT+CGG + chr4.2:9047774-9047793 MS.gene57391:intron 20.0%
! AAATTCAGAAGTTATTGTCT+TGG + chr4.2:9047547-9047566 MS.gene57391:intron 25.0%
! ACAAAAAGTTTCTCTATAGT+CGG - chr4.2:9046577-9046596 None:intergenic 25.0%
! CTATGTTTATAGTAATGCAA+GGG - chr4.2:9046993-9047012 None:intergenic 25.0%
! GACAATAACTTCTGAATTTA+AGG - chr4.2:9047546-9047565 None:intergenic 25.0%
! TCAAATATGTCATAAGGAAA+TGG - chr4.2:9047022-9047041 None:intergenic 25.0%
! TCTATGTTTATAGTAATGCA+AGG - chr4.2:9046994-9047013 None:intergenic 25.0%
!!! AGTACTGATATTGAAATAGT+TGG + chr4.2:9046286-9046305 MS.gene57391:intron 25.0%
ATTTCTTATAGTTACTACCG+AGG - chr4.2:9046328-9046347 None:intergenic 30.0%
CGGTTTCATTAATTGCTTAT+TGG - chr4.2:9047161-9047180 None:intergenic 30.0%
GAATCCAAACATTTCTATTC+CGG - chr4.2:9047721-9047740 None:intergenic 30.0%
TGTTGTTCAGTTATTGTTTC+TGG + chr4.2:9047345-9047364 MS.gene57391:intron 30.0%
TTGTGGTCAAATATGTCATA+AGG - chr4.2:9047028-9047047 None:intergenic 30.0%
TTTCTTATAGTTACTACCGA+GGG - chr4.2:9046327-9046346 None:intergenic 30.0%
! AACTTCTGAATTTAAGGCAA+AGG - chr4.2:9047540-9047559 None:intergenic 30.0%
! TGTGAGATTCTACTTGTTAA+TGG + chr4.2:9046151-9046170 MS.gene57391:intron 30.0%
! TTGTGCAAAATTGATTCAGA+AGG + chr4.2:9047639-9047658 MS.gene57391:CDS 30.0%
!! AACTTTTTGATCTGGGATTA+AGG - chr4.2:9046487-9046506 None:intergenic 30.0%
!! AAGGTTGTACACTATTTCTA+TGG - chr4.2:9047281-9047300 None:intergenic 30.0%
ACAAGTAGAATCTCACAAAC+AGG - chr4.2:9046148-9046167 None:intergenic 35.0%
ACTGCAGTCATAATATCCAT+TGG - chr4.2:9046746-9046765 None:intergenic 35.0%
ATGGTCAAATGAAGCTAATG+AGG + chr4.2:9046461-9046480 MS.gene57391:intron 35.0%
TCATCCGGAATAGAAATGTT+TGG + chr4.2:9047714-9047733 MS.gene57391:intron 35.0%
TCATTTCATGCATCACAGTT+AGG - chr4.2:9047246-9047265 None:intergenic 35.0%
TTGATTCAGAAGGAAAAGCT+AGG + chr4.2:9047649-9047668 MS.gene57391:CDS 35.0%
! AATTTTGCACAACTGCATGA+AGG - chr4.2:9047631-9047650 None:intergenic 35.0%
! CATTGCGATCATTGATTATG+AGG + chr4.2:9046432-9046451 MS.gene57391:intron 35.0%
! GTCAAGGTATGTTTTTCATC+CGG + chr4.2:9047699-9047718 MS.gene57391:intron 35.0%
! TGTTATTGCTTATTGGTGCT+CGG - chr4.2:9047181-9047200 None:intergenic 35.0%
!! ATTGTTTCTGGTGTATTCCT+TGG + chr4.2:9047357-9047376 MS.gene57391:intron 35.0%
!! TTGTTTCTGGTGTATTCCTT+GGG + chr4.2:9047358-9047377 MS.gene57391:intron 35.0%
AAAAACTCAAGACGCACACA+TGG - chr4.2:9046614-9046633 None:intergenic 40.0%
ATTGGCACCTCTCATGATTA+GGG - chr4.2:9047143-9047162 None:intergenic 40.0%
CAACCTTTGAATGTCAAGAG+CGG + chr4.2:9047294-9047313 MS.gene57391:intron 40.0%
TATTGGCACCTCTCATGATT+AGG - chr4.2:9047144-9047163 None:intergenic 40.0%
TTCACCAACTTGACGTAATC+AGG - chr4.2:9046890-9046909 None:intergenic 40.0%
TTCAGAAGGAAAAGCTAGGA+AGG + chr4.2:9047653-9047672 MS.gene57391:CDS 40.0%
TTTCATGCATCACAGTTAGG+AGG - chr4.2:9047243-9047262 None:intergenic 40.0%
! ATTTTTGTAGGATTTCGGCG+AGG + chr4.2:9047779-9047798 MS.gene57391:intron 40.0%
ACAGAGTTCAGAGAGGATAC+AGG - chr4.2:9047486-9047505 None:intergenic 45.0%
ACATACCTTGACAACCACAC+AGG - chr4.2:9047691-9047710 None:intergenic 45.0%
AGAGAGGATACAGGGTAAGA+GGG - chr4.2:9047477-9047496 None:intergenic 45.0%
ATCCTGACCCTAATCATGAG+AGG + chr4.2:9047133-9047152 MS.gene57391:intron 45.0%
ATGCAAAGACCCTTGGAGAA+TGG + chr4.2:9046954-9046973 MS.gene57391:CDS 45.0%
CAGAGTTCAGAGAGGATACA+GGG - chr4.2:9047485-9047504 None:intergenic 45.0%
CCAACCTGATTACGTCAAGT+TGG + chr4.2:9046883-9046902 MS.gene57391:CDS 45.0%
CCAACTTGACGTAATCAGGT+TGG - chr4.2:9046886-9046905 None:intergenic 45.0%
GACACTGTGTTCAGCACAAA+GGG - chr4.2:9046915-9046934 None:intergenic 45.0%
GATCTGATAGTCATAGCCCT+CGG + chr4.2:9046308-9046327 MS.gene57391:intron 45.0%
GATTTGCACCACGATGTTGA+GGG - chr4.2:9047116-9047135 None:intergenic 45.0%
GGAAAAGCTAGGAAGGTAAC+TGG + chr4.2:9047660-9047679 MS.gene57391:CDS 45.0%
GTTCCAAATGCAAAGACCCT+TGG + chr4.2:9046947-9046966 MS.gene57391:CDS 45.0%
TCTCCAAGGGTCTTTGCATT+TGG - chr4.2:9046953-9046972 None:intergenic 45.0%
TGACACTGTGTTCAGCACAA+AGG - chr4.2:9046916-9046935 None:intergenic 45.0%
TGAGGTTACAGAGTTCAGAG+AGG - chr4.2:9047493-9047512 None:intergenic 45.0%
TGCAAAGACCCTTGGAGAAT+GGG + chr4.2:9046955-9046974 MS.gene57391:CDS 45.0%
TTGAGAAAGTCACGCGCTTA+TGG + chr4.2:9046776-9046795 MS.gene57391:CDS 45.0%
!! ATAATATCCATTGGCTCCCC+TGG - chr4.2:9046737-9046756 None:intergenic 45.0%
!!! AAATTAAAAAATTTAATTTG+TGG + chr4.2:9046406-9046425 MS.gene57391:intron 5.0%
ACAGCTCTGTGTTCTTGCTG+AGG + chr4.2:9046853-9046872 MS.gene57391:CDS 50.0%
AGAAAGTCACGCGCTTATGG+TGG + chr4.2:9046779-9046798 MS.gene57391:CDS 50.0%
ATTCCAGCTGCAGCAACAAC+TGG - chr4.2:9046713-9046732 None:intergenic 50.0%
CACCTCTCATGATTAGGGTC+AGG - chr4.2:9047138-9047157 None:intergenic 50.0%
CAGAGAGGATACAGGGTAAG+AGG - chr4.2:9047478-9047497 None:intergenic 50.0%
CCACCGCTCTTGACATTCAA+AGG - chr4.2:9047300-9047319 None:intergenic 50.0%
CCTTTGAATGTCAAGAGCGG+TGG + chr4.2:9047297-9047316 MS.gene57391:intron 50.0%
CTGGTGTATTCCTTGGGTCT+GGG + chr4.2:9047364-9047383 MS.gene57391:intron 50.0%
GAGAGGATACAGGGTAAGAG+GGG - chr4.2:9047476-9047495 None:intergenic 50.0%
GGATTTGCACCACGATGTTG+AGG - chr4.2:9047117-9047136 None:intergenic 50.0%
TACACCAGCCCATTCTCCAA+GGG - chr4.2:9046966-9046985 None:intergenic 50.0%
TATAGTCGGCGAGATTCACG+TGG - chr4.2:9046563-9046582 None:intergenic 50.0%
TCTGGTGTATTCCTTGGGTC+TGG + chr4.2:9047363-9047382 MS.gene57391:intron 50.0%
TGACATCTCCCTCAACATCG+TGG + chr4.2:9047105-9047124 MS.gene57391:intron 50.0%
TGGTGTATTCCTTGGGTCTG+GGG + chr4.2:9047365-9047384 MS.gene57391:intron 50.0%
TTACACCAGCCCATTCTCCA+AGG - chr4.2:9046967-9046986 None:intergenic 50.0%
TTGCTCCTGTGTGGTTGTCA+AGG + chr4.2:9047683-9047702 MS.gene57391:CDS 50.0%
TTGCTGCAGCTGGAATTCCA+GGG + chr4.2:9046717-9046736 MS.gene57391:CDS 50.0%
AAGACCCTTGGAGAATGGGC+TGG + chr4.2:9046959-9046978 MS.gene57391:CDS 55.0%
GGTAACTGGTTGCTCCTGTG+TGG + chr4.2:9047674-9047693 MS.gene57391:CDS 55.0%
GTTGCTGCAGCTGGAATTCC+AGG + chr4.2:9046716-9046735 MS.gene57391:CDS 55.0%
TGCTGCAGCTGGAATTCCAG+GGG + chr4.2:9046718-9046737 MS.gene57391:CDS 55.0%
TGGAATTCCAGGGGAGCCAA+TGG + chr4.2:9046727-9046746 MS.gene57391:CDS 55.0%
TGTATTCCTTGGGTCTGGGG+AGG + chr4.2:9047368-9047387 MS.gene57391:intron 55.0%
! GCTTATGGTGGTCTTGCACG+TGG + chr4.2:9046791-9046810 MS.gene57391:CDS 55.0%
GAGCCAGTTGTTGCTGCAGC+TGG + chr4.2:9046707-9046726 MS.gene57391:CDS 60.0%
AACCAGCCTCCCCAGACCCA+AGG - chr4.2:9047377-9047396 None:intergenic 65.0%
TTCCTTGGGTCTGGGGAGGC+TGG + chr4.2:9047372-9047391 MS.gene57391:intron 65.0%
! TGGGTCTGGGGAGGCTGGTT+CGG + chr4.2:9047377-9047396 MS.gene57391:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 9046113 9047848 9046113 ID=MS.gene57391
chr4.2 mRNA 9046113 9047848 9046113 ID=MS.gene57391.t1;Parent=MS.gene57391
chr4.2 exon 9046113 9046120 9046113 ID=MS.gene57391.t1.exon1;Parent=MS.gene57391.t1
chr4.2 CDS 9046113 9046120 9046113 ID=cds.MS.gene57391.t1;Parent=MS.gene57391.t1
chr4.2 exon 9046685 9046982 9046685 ID=MS.gene57391.t1.exon2;Parent=MS.gene57391.t1
chr4.2 CDS 9046685 9046982 9046685 ID=cds.MS.gene57391.t1;Parent=MS.gene57391.t1
chr4.2 exon 9047639 9047704 9047639 ID=MS.gene57391.t1.exon3;Parent=MS.gene57391.t1
chr4.2 CDS 9047639 9047704 9047639 ID=cds.MS.gene57391.t1;Parent=MS.gene57391.t1
chr4.2 exon 9047789 9047848 9047789 ID=MS.gene57391.t1.exon4;Parent=MS.gene57391.t1
chr4.2 CDS 9047789 9047848 9047789 ID=cds.MS.gene57391.t1;Parent=MS.gene57391.t1
Gene Sequence

>MS.gene57391

ATGTCTGGGTATGTCTATTTTTGCTTAATTTACCTGTTTGTGAGATTCTACTTGTTAATGGTCTATTCATTATCTTATTTTGCAATTTTTTGTTGAATCTATATAGTTTTATTTTCTTTCATGAACTGCGTTTGTAAACTTTCAAATGCATTATAGATTTGATTTTAATTTTAAGTACTGATATTGAAATAGTTGGATCTGATAGTCATAGCCCTCGGTAGTAACTATAAGAAATTTAGAACACAGATAACAGTTTAGTATTTATTTAATTTTCAAAACTATTTTTCAAAATGAAATTAAAAAATTTAATTTGTGGTTGCATTGCGATCATTGATTATGAGGATAATTATGGTCAAATGAAGCTAATGAGGCCTTAATCCCAGATCAAAAAGTTATTATAAACTGCTGTGTATTAGTGTAGACACTTTTCTGTTTCTGTGATTTGAACCACGTGAATCTCGCCGACTATAGAGAAACTTTTTGTTGTGCAATGCTTATCCATGTGTGCGTCTTGAGTTTTTAAGATATTCATTGTTAATTTTCTGACTGTTGATAAACATTTTGTTGTTCAGAGAAGAAGAAGTTGTTGTAGCTGAGCCAGTTGTTGCTGCAGCTGGAATTCCAGGGGAGCCAATGGATATTATGACTGCAGTTCAGCTTGTGTTGAGAAAGTCACGCGCTTATGGTGGTCTTGCACGTGGTCTTCATGAAAGTGCTAAAGTGATTGAGAAGCATGCTGCACAGCTCTGTGTTCTTGCTGAGGATTGTGACCAACCTGATTACGTCAAGTTGGTGAAAGCCCTTTGTGCTGAACACAGTGTCAGTTTGTTGACTGTTCCAAATGCAAAGACCCTTGGAGAATGGGCTGGTGTAAGTGCCCTTGCATTACTATAAACATAGATCAATCCATTTCCTTATGACATATTTGACCACAATTCTCAATTATATATTTTTTGATAAATTCATGAATTACTTTAAAATAAGTGTGGGAGTGACATCTCCCTCAACATCGTGGTGCAAATCCTGACCCTAATCATGAGAGGTGCCAATAAGCAATTAATGAAACCGAGCACCAATAAGCAATAACATTTTATAAATTGCTTCTCTATAGATGTGACTTACTTAAACCTCCTAACTGTGATGCATGAAATGATAAATCTTTCTTCCATAGAAATAGTGTACAACCTTTGAATGTCAAGAGCGGTGGACAGTTCTCAAATTTAGATTATGAATGTTGTTCAGTTATTGTTTCTGGTGTATTCCTTGGGTCTGGGGAGGCTGGTTCGGCAGCTTGTACTGTATTTGCCTTTTGTTTTATATTGGTTTTGTTTTTCTTTGTGATGTGTTTCTGTTTAGAGTACCCCTCTTACCCTGTATCCTCTCTGAACTCTGTAACCTCATTTATTTTAATCTATATGTTTATACCTTTGCCTTAAATTCAGAAGTTATTGTCTTGGTCTTTTTTTTATGCTTTCAAAATCTTATGTCATTCTTTTGTACTGATTTTGTGACGCACCTTCATGCAGTTGTGCAAAATTGATTCAGAAGGAAAAGCTAGGAAGGTAACTGGTTGCTCCTGTGTGGTTGTCAAGGTATGTTTTTCATCCGGAATAGAAATGTTTGGATTCTGTTTTCATCTTCCGTTTATGTATGCTTAACTATTTTCATTTTTGTAGGATTTCGGCGAGGAACATGAAGCATACAACGTTGTTCTTCAGCACGTGAAATCTAACTGA

Protein sequence

>MS.gene57391.t1

MSGEEEVVVAEPVVAAAGIPGEPMDIMTAVQLVLRKSRAYGGLARGLHESAKVIEKHAAQLCVLAEDCDQPDYVKLVKALCAEHSVSLLTVPNAKTLGEWAGLCKIDSEGKARKVTGCSCVVVKDFGEEHEAYNVVLQHVKSN