Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018887.t1 | RHN79104.1 | 98.2 | 944 | 16 | 1 | 1 | 944 | 1 | 943 | 0.00E+00 | 1726.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018887.t1 | O81644 | 71.8 | 941 | 258 | 6 | 6 | 944 | 4 | 939 | 0.0e+00 | 1237.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018887.t1 | A0A396JPH0 | 98.2 | 944 | 16 | 1 | 1 | 944 | 1 | 943 | 0.0e+00 | 1726.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene018887 | MS.gene053110 | PPI |
| MS.gene018887 | MS.gene70077 | PPI |
| MS.gene018887 | MS.gene057841 | PPI |
| MS.gene018887 | MS.gene37274 | PPI |
| MS.gene018887 | MS.gene70072 | PPI |
| MS.gene018887 | MS.gene04628 | PPI |
| MS.gene018887 | MS.gene032698 | PPI |
| MS.gene018887 | MS.gene057833 | PPI |
| MS.gene018887 | MS.gene30959 | PPI |
| MS.gene057838 | MS.gene018887 | PPI |
| MS.gene018887 | MS.gene45493 | PPI |
| MS.gene31154 | MS.gene018887 | PPI |
| MS.gene018887 | MS.gene70078 | PPI |
| MS.gene018887 | MS.gene052120 | PPI |
| MS.gene018887 | MS.gene52664 | PPI |
| MS.gene018887 | MS.gene49850 | PPI |
| MS.gene018887 | MS.gene37081 | PPI |
| MS.gene018887 | MS.gene055991 | PPI |
| MS.gene45491 | MS.gene018887 | PPI |
| MS.gene018887 | MS.gene000108 | PPI |
| MS.gene018887 | MS.gene037502 | PPI |
| MS.gene82765 | MS.gene018887 | PPI |
| MS.gene034501 | MS.gene018887 | PPI |
| MS.gene017992 | MS.gene018887 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018887.t1 | MTR_7g091460 | 79.053 | 950 | 189 | 6 | 1 | 944 | 1 | 946 | 0.0 | 1479 |
| MS.gene018887.t1 | MTR_4g127660 | 45.838 | 949 | 469 | 11 | 8 | 944 | 8 | 923 | 0.0 | 846 |
| MS.gene018887.t1 | MTR_2g036860 | 46.002 | 963 | 463 | 12 | 1 | 944 | 1 | 925 | 0.0 | 830 |
| MS.gene018887.t1 | MTR_3g463530 | 43.218 | 752 | 417 | 5 | 6 | 757 | 6 | 747 | 0.0 | 650 |
| MS.gene018887.t1 | MTR_3g463530 | 43.218 | 752 | 417 | 5 | 6 | 757 | 6 | 747 | 0.0 | 650 |
| MS.gene018887.t1 | MTR_3g463530 | 43.681 | 728 | 402 | 4 | 1 | 728 | 1 | 720 | 0.0 | 634 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018887.t1 | AT2G41740 | 70.370 | 945 | 265 | 9 | 6 | 944 | 4 | 939 | 0.0 | 1324 |
| MS.gene018887.t1 | AT2G41740 | 70.370 | 945 | 265 | 9 | 6 | 944 | 4 | 939 | 0.0 | 1324 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 70.233 | 944 | 265 | 5 | 1 | 944 | 1 | 928 | 0.0 | 1301 |
| MS.gene018887.t1 | AT3G57410 | 74.492 | 788 | 198 | 2 | 1 | 788 | 1 | 785 | 0.0 | 1231 |
| MS.gene018887.t1 | AT3G57410 | 76.389 | 720 | 170 | 0 | 1 | 720 | 1 | 720 | 0.0 | 1183 |
| MS.gene018887.t1 | AT4G30160 | 46.833 | 963 | 467 | 11 | 1 | 944 | 1 | 937 | 0.0 | 852 |
| MS.gene018887.t1 | AT4G30160 | 46.833 | 963 | 467 | 11 | 1 | 944 | 1 | 937 | 0.0 | 852 |
| MS.gene018887.t1 | AT4G30160 | 46.833 | 963 | 467 | 11 | 1 | 944 | 1 | 937 | 0.0 | 852 |
| MS.gene018887.t1 | AT4G30160 | 46.399 | 972 | 467 | 12 | 1 | 944 | 1 | 946 | 0.0 | 844 |
| MS.gene018887.t1 | AT5G57320 | 43.913 | 961 | 486 | 11 | 1 | 944 | 1 | 925 | 0.0 | 825 |
| MS.gene018887.t1 | AT5G57320 | 43.913 | 961 | 486 | 11 | 1 | 944 | 1 | 925 | 0.0 | 825 |
| MS.gene018887.t1 | AT2G29890 | 42.667 | 750 | 408 | 7 | 1 | 742 | 1 | 736 | 0.0 | 625 |
| MS.gene018887.t1 | AT2G29890 | 42.667 | 750 | 408 | 7 | 1 | 742 | 1 | 736 | 0.0 | 625 |
| MS.gene018887.t1 | AT2G29890 | 42.686 | 752 | 407 | 8 | 1 | 742 | 1 | 738 | 0.0 | 624 |
| MS.gene018887.t1 | AT2G29890 | 42.686 | 752 | 407 | 8 | 1 | 742 | 1 | 738 | 0.0 | 624 |
| MS.gene018887.t1 | AT2G29890 | 42.133 | 750 | 395 | 9 | 1 | 742 | 1 | 719 | 0.0 | 601 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6950 | gene | 20789 | 41176 | 20789 | ID=MS.gene018887 |
| 6950 | mRNA | 20789 | 41176 | 20789 | ID=MS.gene018887.t1;Parent=MS.gene018887 |
| 6950 | exon | 20789 | 20844 | 20789 | ID=MS.gene018887.t1.exon1;Parent=MS.gene018887.t1 |
| 6950 | CDS | 20789 | 20844 | 20789 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 21323 | 21422 | 21323 | ID=MS.gene018887.t1.exon2;Parent=MS.gene018887.t1 |
| 6950 | CDS | 21323 | 21422 | 21323 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 22081 | 22149 | 22081 | ID=MS.gene018887.t1.exon3;Parent=MS.gene018887.t1 |
| 6950 | CDS | 22081 | 22149 | 22081 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 22365 | 22601 | 22365 | ID=MS.gene018887.t1.exon4;Parent=MS.gene018887.t1 |
| 6950 | CDS | 22365 | 22601 | 22365 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 22671 | 22848 | 22671 | ID=MS.gene018887.t1.exon5;Parent=MS.gene018887.t1 |
| 6950 | CDS | 22671 | 22848 | 22671 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 22943 | 23075 | 22943 | ID=MS.gene018887.t1.exon6;Parent=MS.gene018887.t1 |
| 6950 | CDS | 22943 | 23075 | 22943 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 31629 | 31788 | 31629 | ID=MS.gene018887.t1.exon7;Parent=MS.gene018887.t1 |
| 6950 | CDS | 31629 | 31788 | 31629 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 31866 | 32016 | 31866 | ID=MS.gene018887.t1.exon8;Parent=MS.gene018887.t1 |
| 6950 | CDS | 31866 | 32016 | 31866 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 32095 | 32195 | 32095 | ID=MS.gene018887.t1.exon9;Parent=MS.gene018887.t1 |
| 6950 | CDS | 32095 | 32195 | 32095 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 32280 | 32438 | 32280 | ID=MS.gene018887.t1.exon10;Parent=MS.gene018887.t1 |
| 6950 | CDS | 32280 | 32438 | 32280 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 34323 | 34391 | 34323 | ID=MS.gene018887.t1.exon11;Parent=MS.gene018887.t1 |
| 6950 | CDS | 34323 | 34391 | 34323 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 34463 | 34531 | 34463 | ID=MS.gene018887.t1.exon12;Parent=MS.gene018887.t1 |
| 6950 | CDS | 34463 | 34531 | 34463 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 34619 | 34756 | 34619 | ID=MS.gene018887.t1.exon13;Parent=MS.gene018887.t1 |
| 6950 | CDS | 34619 | 34756 | 34619 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 34854 | 34982 | 34854 | ID=MS.gene018887.t1.exon14;Parent=MS.gene018887.t1 |
| 6950 | CDS | 34854 | 34982 | 34854 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 37069 | 37216 | 37069 | ID=MS.gene018887.t1.exon15;Parent=MS.gene018887.t1 |
| 6950 | CDS | 37069 | 37216 | 37069 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 37351 | 37361 | 37351 | ID=MS.gene018887.t1.exon16;Parent=MS.gene018887.t1 |
| 6950 | CDS | 37351 | 37361 | 37351 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 37483 | 37623 | 37483 | ID=MS.gene018887.t1.exon17;Parent=MS.gene018887.t1 |
| 6950 | CDS | 37483 | 37623 | 37483 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 37705 | 37836 | 37705 | ID=MS.gene018887.t1.exon18;Parent=MS.gene018887.t1 |
| 6950 | CDS | 37705 | 37836 | 37705 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 38177 | 38239 | 38177 | ID=MS.gene018887.t1.exon19;Parent=MS.gene018887.t1 |
| 6950 | CDS | 38177 | 38239 | 38177 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 39728 | 39832 | 39728 | ID=MS.gene018887.t1.exon20;Parent=MS.gene018887.t1 |
| 6950 | CDS | 39728 | 39832 | 39728 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 40210 | 40468 | 40210 | ID=MS.gene018887.t1.exon21;Parent=MS.gene018887.t1 |
| 6950 | CDS | 40210 | 40468 | 40210 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| 6950 | exon | 40953 | 41176 | 40953 | ID=MS.gene018887.t1.exon22;Parent=MS.gene018887.t1 |
| 6950 | CDS | 40953 | 41176 | 40953 | ID=cds.MS.gene018887.t1;Parent=MS.gene018887.t1 |
| Gene Sequence |
| Protein sequence |
