AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30959.t1 MTR_2g096840 100.000 377 0 0 1 377 1 377 0.0 784
MS.gene30959.t1 MTR_0015s0130 98.674 377 5 0 1 377 1 377 0.0 776
MS.gene30959.t1 MTR_7g026230 97.082 377 11 0 1 377 1 377 0.0 768
MS.gene30959.t1 MTR_6g004000 97.082 377 11 0 1 377 1 377 0.0 768
MS.gene30959.t1 MTR_7g093260 96.021 377 15 0 1 377 1 377 0.0 765
MS.gene30959.t1 MTR_3g095530 94.960 377 19 0 1 377 1 377 0.0 761
MS.gene30959.t1 MTR_2g008050 93.634 377 24 0 1 377 1 377 0.0 752
MS.gene30959.t1 MTR_7g027185 42.480 379 204 3 10 374 7 385 1.41e-118 349
MS.gene30959.t1 MTR_7g027185 43.662 355 187 2 10 351 7 361 7.33e-116 341
MS.gene30959.t1 MTR_7g027185 46.580 307 162 1 10 314 7 313 2.75e-108 320
MS.gene30959.t1 MTR_5g066400 37.360 447 206 11 1 376 1 444 4.42e-93 286
MS.gene30959.t1 MTR_8g464900 36.574 432 201 10 1 362 1 429 1.71e-86 268
MS.gene30959.t1 MTR_5g066400 34.753 446 191 11 1 376 1 416 2.52e-80 252
MS.gene30959.t1 MTR_8g089630 34.550 411 219 8 10 372 10 418 5.49e-72 231
MS.gene30959.t1 MTR_5g066400 34.250 400 192 10 1 332 1 397 4.11e-69 223
MS.gene30959.t1 MTR_3g030550 37.105 380 213 9 7 377 1 363 1.07e-67 218
MS.gene30959.t1 MTR_2g445210 28.132 423 238 7 8 366 6 426 3.20e-55 188
MS.gene30959.t1 MTR_8g007440 35.227 264 144 7 77 319 9 266 3.45e-46 159
MS.gene30959.t1 MTR_2g445210 26.613 372 207 7 8 315 6 375 3.38e-41 149
MS.gene30959.t1 MTR_3g010170 30.303 297 183 9 91 371 179 467 2.14e-40 149
MS.gene30959.t1 MTR_8g070520 31.058 293 175 10 91 366 187 469 7.96e-36 136
MS.gene30959.t1 MTR_8g070520 30.576 278 167 9 91 352 187 454 1.62e-32 127
MS.gene30959.t1 MTR_3g067945 28.968 252 160 7 9 245 21 268 9.70e-22 97.4
MS.gene30959.t1 MTR_3g067945 33.607 122 78 2 250 368 586 707 4.69e-16 80.1
MS.gene30959.t1 MTR_8g008540 70.270 37 11 0 340 376 41 77 4.15e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30959.t1 AT3G12110 96.552 377 13 0 1 377 1 377 0.0 765
MS.gene30959.t1 AT3G53750 95.756 377 16 0 1 377 1 377 0.0 763
MS.gene30959.t1 AT3G53750 95.756 377 16 0 1 377 1 377 0.0 763
MS.gene30959.t1 AT2G37620 95.756 377 16 0 1 377 1 377 0.0 763
MS.gene30959.t1 AT2G37620 95.756 377 16 0 1 377 1 377 0.0 763
MS.gene30959.t1 AT2G37620 95.756 377 16 0 1 377 1 377 0.0 763
MS.gene30959.t1 AT2G37620 95.756 377 16 0 1 377 1 377 0.0 763
MS.gene30959.t1 AT5G59370 96.817 377 12 0 1 377 1 377 0.0 762
MS.gene30959.t1 AT5G59370 96.817 377 12 0 1 377 1 377 0.0 762
MS.gene30959.t1 AT3G46520 96.552 377 13 0 1 377 1 377 0.0 761
MS.gene30959.t1 AT3G46520 96.552 377 13 0 1 377 1 377 0.0 761
MS.gene30959.t1 AT5G09810 94.960 377 19 0 1 377 1 377 0.0 759
MS.gene30959.t1 AT1G49240 92.573 377 28 0 1 377 1 377 0.0 745
MS.gene30959.t1 AT3G18780 92.308 377 29 0 1 377 1 377 0.0 744
MS.gene30959.t1 AT3G18780 92.308 377 29 0 1 377 1 377 0.0 744
MS.gene30959.t1 AT3G18780 92.201 359 28 0 1 359 1 359 0.0 703
MS.gene30959.t1 AT2G42100 83.967 368 59 0 10 377 11 378 0.0 673
MS.gene30959.t1 AT2G42090 70.000 370 106 1 7 376 1 365 0.0 564
MS.gene30959.t1 AT2G42170 77.108 332 73 1 46 377 1 329 0.0 556
MS.gene30959.t1 AT2G42170 77.108 332 73 1 46 377 1 329 0.0 556
MS.gene30959.t1 AT2G42170 76.923 312 69 1 46 357 1 309 0.0 518
MS.gene30959.t1 AT2G42170 76.923 312 69 1 46 357 1 309 0.0 518
MS.gene30959.t1 AT2G42170 76.991 226 49 1 152 377 9 231 2.18e-130 373
MS.gene30959.t1 AT3G27000 43.536 379 200 4 10 374 7 385 4.89e-121 355
MS.gene30959.t1 AT1G18450 37.472 443 207 9 1 376 1 440 1.50e-95 292
MS.gene30959.t1 AT1G13180 34.223 412 219 9 10 372 9 417 2.76e-72 232
MS.gene30959.t1 AT3G60830 37.467 379 213 9 7 377 1 363 5.82e-67 216
MS.gene30959.t1 AT3G33520 30.166 421 236 7 10 377 5 420 5.44e-58 194
MS.gene30959.t1 AT5G56180 31.338 284 170 9 91 358 50 324 1.95e-35 132
MS.gene30959.t1 AT5G56180 31.338 284 170 9 91 358 182 456 1.19e-34 133
MS.gene30959.t1 AT3G12380 29.183 257 159 6 9 245 21 274 1.21e-24 106
MS.gene30959.t1 AT3G12380 32.738 168 106 4 207 368 550 716 3.86e-17 83.6
MS.gene30959.t1 AT3G12380 29.020 255 156 6 9 245 21 268 4.83e-24 104
MS.gene30959.t1 AT3G12380 32.738 168 106 4 207 368 544 710 3.74e-17 83.6
MS.gene30959.t1 AT1G73910 35.862 145 74 3 1 129 1 142 2.08e-23 95.1
MS.gene30959.t1 AT1G73910 35.862 145 74 3 1 129 1 142 2.08e-23 95.1
MS.gene30959.t1 AT5G56180 32.178 202 114 8 91 281 182 371 2.08e-19 89.4
MS.gene30959.t1 AT1G73910 29.268 123 71 2 1 107 1 123 3.00e-12 64.7

Find 105 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGATCTTCATGCTGCTT+GGG 0.250595 2.2:+5278640 None:intergenic
TGCTATGTATGTTGCTATTC+AGG 0.269599 2.2:-5279445 MS.gene30959:CDS
AGCAAAACCGGGTGCTCCTC+TGG 0.272892 2.2:+5279543 None:intergenic
CAACCACTGGTCTGTGATAA+TGG 0.278942 2.2:-5280147 MS.gene30959:CDS
AACATTTAGGCTGGATTTGC+TGG 0.289528 2.2:-5279789 MS.gene30959:intron
CACACGGAGTTCATTGTAAA+AGG 0.330285 2.2:+5279569 None:intergenic
ACCTTGATCTTCATGCTGCT+TGG 0.336588 2.2:+5278639 None:intergenic
AGCATGGTATTGTGAGTAAC+TGG 0.340011 2.2:-5279620 MS.gene30959:CDS
CGATTAGCCTTTGGGTTAAG+AGG 0.374736 2.2:+5279510 None:intergenic
GGTGCTTCAGTGAGCAAAAC+CGG 0.388529 2.2:+5279531 None:intergenic
AATATGTACAGGTATTGTTC+TGG 0.396337 2.2:-5279165 MS.gene30959:intron
CTCACAATACCATGCTCAAT+TGG 0.397001 2.2:+5279627 None:intergenic
CAAGCTCTTGCTCAAAATCA+AGG 0.399460 2.2:+5278943 None:intergenic
ATCCTTCGTCTTGATTTAGC+AGG 0.412905 2.2:-5279080 MS.gene30959:CDS
GGGATGACATGGAAAAGATT+TGG 0.422607 2.2:-5279599 MS.gene30959:CDS
CCGAGAGAAAATACAGTGTC+TGG 0.425088 2.2:-5278605 MS.gene30959:CDS
GCAAAACCGGGTGCTCCTCT+GGG 0.429592 2.2:+5279544 None:intergenic
CTCATTCTGTCAGCAATACC+AGG 0.431489 2.2:+5278684 None:intergenic
TACAATTTCACGCTCGGCTG+TGG 0.436320 2.2:+5278998 None:intergenic
CATTTCCTGTGTACAATTGA+AGG 0.448729 2.2:+5278373 None:intergenic
AGTGCTAAGAGAAGCTAAGA+TGG 0.454030 2.2:+5278572 None:intergenic
TATGCCAGTGGTCGAACAAC+CGG 0.454712 2.2:-5279408 MS.gene30959:intron
CTTGATCTTCATGCTGCTTG+GGG 0.455780 2.2:+5278641 None:intergenic
AGGGCATATCCTTCATAGAT+AGG 0.458717 2.2:+5279113 None:intergenic
GATGGACAGATCATCACTAT+TGG 0.461294 2.2:-5278873 MS.gene30959:CDS
ATCTCTTACAATTTCACGCT+CGG 0.471407 2.2:+5278992 None:intergenic
TAATGGAACAGGAATGGTTA+AGG 0.478719 2.2:-5280130 MS.gene30959:intron
CTGGTCTGTGATAATGGAAC+AGG 0.481376 2.2:-5280141 MS.gene30959:CDS
AAATACAGTGTCTGGATTGG+TGG 0.485676 2.2:-5278597 MS.gene30959:CDS
CCTGTGTACAATTGAAGGAC+CGG 0.488592 2.2:+5278378 None:intergenic
CCAGCCTTCCATGGTTGGAA+TGG 0.489084 2.2:-5278817 MS.gene30959:CDS
GCAGTGTTTCCTAGCATTGT+TGG 0.492238 2.2:-5279750 MS.gene30959:CDS
ACAGGTATTGTTCTGGACTC+TGG 0.493483 2.2:-5279158 MS.gene30959:intron
CACCGGTGTGATGGTTGGCA+TGG 0.497309 2.2:-5279715 MS.gene30959:CDS
GATATAAGAAAGGACCTGTA+TGG 0.499084 2.2:-5278741 MS.gene30959:CDS
AAGAATAACCACGCTCAGTA+AGG 0.500465 2.2:+5279027 None:intergenic
AGAAAATACAGTGTCTGGAT+TGG 0.510090 2.2:-5278600 MS.gene30959:CDS
TATGGAAACATTGTCCTTAG+TGG 0.510647 2.2:-5278723 MS.gene30959:CDS
CCATTCCAACCATGGAAGGC+TGG 0.515654 2.2:+5278817 None:intergenic
TAGAGTCAATATACCACGCT+TGG 0.516667 2.2:+5279656 None:intergenic
ATAGTGATGATCTGTCCATC+AGG 0.522284 2.2:+5278876 None:intergenic
GGTGGTACTACTATGTTCCC+TGG 0.522338 2.2:-5278702 MS.gene30959:CDS
CGCCACACCGGTGTGATGGT+TGG 0.522369 2.2:-5279720 MS.gene30959:CDS
ATGCTAGGAAACACTGCCCT+TGG 0.524407 2.2:+5279756 None:intergenic
ATGAAAATCCTTACTGAGCG+TGG 0.531509 2.2:-5279035 MS.gene30959:CDS
GTGCGATGTTGATATAAGAA+AGG 0.541027 2.2:-5278751 MS.gene30959:CDS
GACCAGTGGTTGAATATCCT+CGG 0.542292 2.2:+5280158 None:intergenic
CGCCGAGGATATTCAACCAC+TGG 0.542623 2.2:-5280160 MS.gene30959:CDS
CCCAAGCAGCATGAAGATCA+AGG 0.543751 2.2:-5278640 MS.gene30959:CDS
TCCTTCGTCTTGATTTAGCA+GGG 0.546953 2.2:-5279079 MS.gene30959:CDS
TAAGGACAATGTTTCCATAC+AGG 0.547731 2.2:+5278727 None:intergenic
CACACTGTACCTATCTATGA+AGG 0.549436 2.2:-5279122 MS.gene30959:CDS
ATTGTTCTGGACTCTGGAGA+TGG 0.549481 2.2:-5279152 MS.gene30959:CDS
ACCACGCCACACCGGTGTGA+TGG 0.549609 2.2:-5279724 MS.gene30959:CDS
TGAAATTGTAAGAGATGTGA+AGG 0.550481 2.2:-5278985 MS.gene30959:CDS
AAGAGCTATGAGCTTCCTGA+TGG 0.551848 2.2:-5278891 MS.gene30959:CDS
CGTGGTCGACCAACAATGCT+AGG 0.552232 2.2:+5279741 None:intergenic
GAAGGCTGGAAAAGAACCTC+GGG 0.557007 2.2:+5278831 None:intergenic
TGCTGGAGATGATGCTCCAA+GGG 0.557554 2.2:-5279772 MS.gene30959:CDS
ACATAGTAGTACCACCACTA+AGG 0.559599 2.2:+5278709 None:intergenic
ACCATCACACCGGTGTGGCG+TGG 0.560434 2.2:+5279723 None:intergenic
GACGAAGGATAGCATGTGGA+AGG 0.560879 2.2:+5279093 None:intergenic
CTAACCGGTTGTTCGACCAC+TGG 0.563611 2.2:+5279404 None:intergenic
AAGGCCGAGTACGATGAATC+CGG 0.564635 2.2:-5278397 MS.gene30959:CDS
AAGCTCTTGCTCAAAATCAA+GGG 0.566750 2.2:+5278944 None:intergenic
TGAATAGCAACATACATAGC+AGG 0.568288 2.2:+5279447 None:intergenic
CTAAAATATCCAATTGAGCA+TGG 0.574515 2.2:-5279636 MS.gene30959:CDS
TGTGGCCCCAGAGGAGCACC+CGG 0.576326 2.2:-5279550 MS.gene30959:CDS
GCTTCCATTCCAACCATGGA+AGG 0.580286 2.2:+5278813 None:intergenic
GGTGCTCCTCTGGGGCCACA+CGG 0.580962 2.2:+5279553 None:intergenic
TTGCTGGAGATGATGCTCCA+AGG 0.581680 2.2:-5279773 MS.gene30959:CDS
AACAGCTTCCATTCCAACCA+TGG 0.591828 2.2:+5278809 None:intergenic
ACCGGTGTGATGGTTGGCAT+GGG 0.591983 2.2:-5279714 MS.gene30959:CDS
GAGCAAGGAAATTACCGCGT+TGG 0.592403 2.2:-5278664 MS.gene30959:CDS
TGTGAGTAACTGGGATGACA+TGG 0.598035 2.2:-5279610 MS.gene30959:CDS
GTGCTTCAGTGAGCAAAACC+GGG 0.598950 2.2:+5279532 None:intergenic
GGAAAAGAACCTCGGGACAA+CGG 0.600966 2.2:+5278838 None:intergenic
ATGGTTGGAATGGAAGCTGT+TGG 0.612805 2.2:-5278807 MS.gene30959:CDS
GGAAGGCTGGAAAAGAACCT+CGG 0.619762 2.2:+5278830 None:intergenic
CATGCTGCTTGGGGCCAACG+CGG 0.625414 2.2:+5278650 None:intergenic
CTGTGATAATGGAACAGGAA+TGG 0.632340 2.2:-5280136 MS.gene30959:CDS
TGAGCGTTTCCGTTGTCCCG+AGG 0.634642 2.2:-5278847 MS.gene30959:CDS
GGAAACATTGTCCTTAGTGG+TGG 0.635690 2.2:-5278720 MS.gene30959:CDS
ACTGGCATAGAGCGATAGAA+CGG 0.638060 2.2:+5279422 None:intergenic
CAAAACCGGGTGCTCCTCTG+GGG 0.638105 2.2:+5279545 None:intergenic
TCAAGACGAAGGATAGCATG+TGG 0.640472 2.2:+5279089 None:intergenic
TGCCAACCATCACACCGGTG+TGG 0.648692 2.2:+5279718 None:intergenic
TGAACTCCGTGTGGCCCCAG+AGG 0.648993 2.2:-5279559 MS.gene30959:CDS
TATTGCTGACAGAATGAGCA+AGG 0.649012 2.2:-5278679 MS.gene30959:CDS
AAAGACAATGGCAGACGCCG+AGG 0.653794 2.2:-5280175 None:intergenic
GTTGGTCGACCACGCCACAC+CGG 0.654816 2.2:-5279732 MS.gene30959:CDS
TCCCATGCCAACCATCACAC+CGG 0.656603 2.2:+5279713 None:intergenic
CCGGTCCTTCAATTGTACAC+AGG 0.656706 2.2:-5278378 MS.gene30959:CDS
ACGAAGGATAGCATGTGGAA+GGG 0.659324 2.2:+5279094 None:intergenic
GATGAAGCTCAGTCCAAGCG+TGG 0.663966 2.2:-5279669 MS.gene30959:CDS
AATGCAGATGTGGATTGCAA+AGG 0.664746 2.2:-5278416 MS.gene30959:intron
AGGACCGGATTCATCGTACT+CGG 0.665643 2.2:+5278393 None:intergenic
TGAAGCACCTCTTAACCCAA+AGG 0.668440 2.2:-5279517 MS.gene30959:CDS
GTTCTATCGCTCTATGCCAG+TGG 0.672809 2.2:-5279420 MS.gene30959:CDS
GTTCCATTATCACAGACCAG+TGG 0.693343 2.2:+5280144 None:intergenic
GCATGGTATTGTGAGTAACT+GGG 0.709006 2.2:-5279619 MS.gene30959:CDS
CTTCATAGATAGGTACAGTG+TGG 0.710370 2.2:+5279123 None:intergenic
GCCCTGCTAAATCAAGACGA+AGG 0.714666 2.2:+5279078 None:intergenic
TCATTCTGTCAGCAATACCA+GGG 0.715629 2.2:+5278685 None:intergenic
AAGCAGCATGAAGATCAAGG+TGG 0.767710 2.2:-5278637 MS.gene30959:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGATTTCAAAGAATT+TGG - chr2.2:5279291-5279310 MS.gene30959:intron 15.0%
!! AACTCTATATTAATATGTAC+AGG - chr2.2:5279358-5279377 MS.gene30959:intron 20.0%
!! GAAGAAAAAAAAGAATTCTA+AGG + chr2.2:5280042-5280061 None:intergenic 20.0%
!!! ACACATTAGTTTTCTTAAAT+CGG + chr2.2:5279163-5279182 None:intergenic 20.0%
!!! CATTCTAAGTTTTTTTATAC+TGG - chr2.2:5279201-5279220 MS.gene30959:intron 20.0%
!!! CTTACATGTTTGATTTATTT+GGG - chr2.2:5280061-5280080 MS.gene30959:intron 20.0%
!!! TCTTACATGTTTGATTTATT+TGG - chr2.2:5280060-5280079 MS.gene30959:intron 20.0%
!! AGTACTAAATGTTGTTGTTA+AGG - chr2.2:5278580-5278599 MS.gene30959:CDS 25.0%
!! TATCTTTTCTGTTTTCTCTT+CGG - chr2.2:5278485-5278504 MS.gene30959:intron 25.0%
!!! AGAATTTGGCTCTATATTTA+TGG - chr2.2:5279305-5279324 MS.gene30959:intron 25.0%
!!! ATTTTTTGAAAATGCAGATG+TGG - chr2.2:5280108-5280127 MS.gene30959:intron 25.0%
!!! CACATTAGTTTTCTTAAATC+GGG + chr2.2:5279162-5279181 None:intergenic 25.0%
AAAAATAAGCATGCATTGCA+AGG - chr2.2:5279229-5279248 MS.gene30959:intron 30.0%
CTAAAATATCCAATTGAGCA+TGG - chr2.2:5278898-5278917 MS.gene30959:CDS 30.0%
TGAAATTGTAAGAGATGTGA+AGG - chr2.2:5279549-5279568 MS.gene30959:CDS 30.0%
TTAAGAAAACTAATGTGTCG+CGG - chr2.2:5279165-5279184 MS.gene30959:intron 30.0%
TTCACAGCAAATTAGTAGAA+AGG + chr2.2:5278705-5278724 None:intergenic 30.0%
! AATATGTACAGGTATTGTTC+TGG - chr2.2:5279369-5279388 MS.gene30959:intron 30.0%
! CATTGTGATTTGCAACATTT+AGG - chr2.2:5278732-5278751 MS.gene30959:CDS 30.0%
!!! CAATGCATGCTTATTTTTAC+TGG + chr2.2:5279227-5279246 None:intergenic 30.0%
AAGCTCTTGCTCAAAATCAA+GGG + chr2.2:5279593-5279612 None:intergenic 35.0%
AGAAAATACAGTGTCTGGAT+TGG - chr2.2:5279934-5279953 MS.gene30959:intron 35.0%
ATCTCTTACAATTTCACGCT+CGG + chr2.2:5279545-5279564 None:intergenic 35.0%
CACGGACAATGTGAATATAT+CGG + chr2.2:5280005-5280024 None:intergenic 35.0%
CATTTCCTGTGTACAATTGA+AGG + chr2.2:5280164-5280183 None:intergenic 35.0%
GATATAAGAAAGGACCTGTA+TGG - chr2.2:5279793-5279812 MS.gene30959:intron 35.0%
GGACAAAAAGATGCATATGT+TGG - chr2.2:5278841-5278860 MS.gene30959:CDS 35.0%
GTGCGATGTTGATATAAGAA+AGG - chr2.2:5279783-5279802 MS.gene30959:CDS 35.0%
TAAATCGGGAAATGACTAAC+CGG + chr2.2:5279148-5279167 None:intergenic 35.0%
TAAGGACAATGTTTCCATAC+AGG + chr2.2:5279810-5279829 None:intergenic 35.0%
TAATGGAACAGGAATGGTTA+AGG - chr2.2:5278404-5278423 MS.gene30959:CDS 35.0%
TATGGAAACATTGTCCTTAG+TGG - chr2.2:5279811-5279830 MS.gene30959:intron 35.0%
TGAATAGCAACATACATAGC+AGG + chr2.2:5279090-5279109 None:intergenic 35.0%
! CTCAGTAAGGATTTTCATCA+AGG + chr2.2:5279497-5279516 None:intergenic 35.0%
! TGCTAATGGATGGAAAAAGA+GGG - chr2.2:5279259-5279278 MS.gene30959:intron 35.0%
! TGCTATGTATGTTGCTATTC+AGG - chr2.2:5279089-5279108 MS.gene30959:CDS 35.0%
! TTCTCTTAGCACTTTTCAAC+AGG - chr2.2:5279972-5279991 MS.gene30959:intron 35.0%
! TTGCTAATGGATGGAAAAAG+AGG - chr2.2:5279258-5279277 MS.gene30959:intron 35.0%
! TTTTTCCTGAAGAAGCGATT+TGG - chr2.2:5278457-5278476 MS.gene30959:intron 35.0%
!!! ATTTTCTCACGATTAGCCTT+TGG + chr2.2:5279036-5279055 None:intergenic 35.0%
!!! TTTTCTCACGATTAGCCTTT+GGG + chr2.2:5279035-5279054 None:intergenic 35.0%
AACATTTAGGCTGGATTTGC+TGG - chr2.2:5278745-5278764 MS.gene30959:CDS 40.0%
AAGAATAACCACGCTCAGTA+AGG + chr2.2:5279510-5279529 None:intergenic 40.0%
AAGGCGTGCATAAATACACA+CGG + chr2.2:5280023-5280042 None:intergenic 40.0%
ACATAGTAGTACCACCACTA+AGG + chr2.2:5279828-5279847 None:intergenic 40.0%
AGCATGGTATTGTGAGTAAC+TGG - chr2.2:5278914-5278933 MS.gene30959:CDS 40.0%
AGGGCATATCCTTCATAGAT+AGG + chr2.2:5279424-5279443 None:intergenic 40.0%
AGTGCTAAGAGAAGCTAAGA+TGG + chr2.2:5279965-5279984 None:intergenic 40.0%
ATAGTGATGATCTGTCCATC+AGG + chr2.2:5279661-5279680 None:intergenic 40.0%
ATCCTTCGTCTTGATTTAGC+AGG - chr2.2:5279454-5279473 MS.gene30959:CDS 40.0%
ATGAAAATCCTTACTGAGCG+TGG - chr2.2:5279499-5279518 MS.gene30959:CDS 40.0%
CAAGCTCTTGCTCAAAATCA+AGG + chr2.2:5279594-5279613 None:intergenic 40.0%
CACACGGAGTTCATTGTAAA+AGG + chr2.2:5278968-5278987 None:intergenic 40.0%
CACACTGTACCTATCTATGA+AGG - chr2.2:5279412-5279431 MS.gene30959:CDS 40.0%
CTCACAATACCATGCTCAAT+TGG + chr2.2:5278910-5278929 None:intergenic 40.0%
CTTCATAGATAGGTACAGTG+TGG + chr2.2:5279414-5279433 None:intergenic 40.0%
GATGGACAGATCATCACTAT+TGG - chr2.2:5279661-5279680 MS.gene30959:CDS 40.0%
GCATGGTATTGTGAGTAACT+GGG - chr2.2:5278915-5278934 MS.gene30959:CDS 40.0%
GGGATGACATGGAAAAGATT+TGG - chr2.2:5278935-5278954 MS.gene30959:CDS 40.0%
TAGAGTCAATATACCACGCT+TGG + chr2.2:5278881-5278900 None:intergenic 40.0%
TATTGCTGACAGAATGAGCA+AGG - chr2.2:5279855-5279874 MS.gene30959:intron 40.0%
TCATTCTGTCAGCAATACCA+GGG + chr2.2:5279852-5279871 None:intergenic 40.0%
TCCTTCGTCTTGATTTAGCA+GGG - chr2.2:5279455-5279474 MS.gene30959:CDS 40.0%
! AAATACAGTGTCTGGATTGG+TGG - chr2.2:5279937-5279956 MS.gene30959:intron 40.0%
! AATGCAGATGTGGATTGCAA+AGG - chr2.2:5280118-5280137 MS.gene30959:intron 40.0%
! CAGACACTGTATTTTCTCTC+GGG + chr2.2:5279931-5279950 None:intergenic 40.0%
! CCAGACACTGTATTTTCTCT+CGG + chr2.2:5279932-5279951 None:intergenic 40.0%
! CTTTTACAATGAACTCCGTG+TGG - chr2.2:5278966-5278985 MS.gene30959:CDS 40.0%
! GTGATTTGCAACATTTAGGC+TGG - chr2.2:5278736-5278755 MS.gene30959:CDS 40.0%
! TCTTTTCTACGATCTGCGAT+TGG - chr2.2:5278654-5278673 MS.gene30959:CDS 40.0%
!! AGGTTCAGTTTGCTAATGGA+TGG - chr2.2:5279249-5279268 MS.gene30959:intron 40.0%
!! CTGTGATAATGGAACAGGAA+TGG - chr2.2:5278398-5278417 MS.gene30959:CDS 40.0%
!! TGCAAGGTTCAGTTTGCTAA+TGG - chr2.2:5279245-5279264 MS.gene30959:intron 40.0%
!! TTTTCATCAAGGCATCAGTG+AGG + chr2.2:5279486-5279505 None:intergenic 40.0%
AACAGCTTCCATTCCAACCA+TGG + chr2.2:5279728-5279747 None:intergenic 45.0%
AAGAGCTATGAGCTTCCTGA+TGG - chr2.2:5279643-5279662 MS.gene30959:CDS 45.0%
AAGCAGCATGAAGATCAAGG+TGG - chr2.2:5279897-5279916 MS.gene30959:intron 45.0%
ACCTTGATCTTCATGCTGCT+TGG + chr2.2:5279898-5279917 None:intergenic 45.0%
ACGAAGGATAGCATGTGGAA+GGG + chr2.2:5279443-5279462 None:intergenic 45.0%
ACTGGCATAGAGCGATAGAA+CGG + chr2.2:5279115-5279134 None:intergenic 45.0%
ATGGTTGGAATGGAAGCTGT+TGG - chr2.2:5279727-5279746 MS.gene30959:CDS 45.0%
CATAGCCAAATCGCTTCTTC+AGG + chr2.2:5278465-5278484 None:intergenic 45.0%
CCGAGAGAAAATACAGTGTC+TGG - chr2.2:5279929-5279948 MS.gene30959:intron 45.0%
CCTGTGTACAATTGAAGGAC+CGG + chr2.2:5280159-5280178 None:intergenic 45.0%
CTCATTCTGTCAGCAATACC+AGG + chr2.2:5279853-5279872 None:intergenic 45.0%
GACCAGTGGTTGAATATCCT+CGG + chr2.2:5278379-5278398 None:intergenic 45.0%
GCAGTGTTTCCTAGCATTGT+TGG - chr2.2:5278784-5278803 MS.gene30959:CDS 45.0%
GGAAACATTGTCCTTAGTGG+TGG - chr2.2:5279814-5279833 MS.gene30959:intron 45.0%
GTTCCATTATCACAGACCAG+TGG + chr2.2:5278393-5278412 None:intergenic 45.0%
TCAAGACGAAGGATAGCATG+TGG + chr2.2:5279448-5279467 None:intergenic 45.0%
TGTGAGTAACTGGGATGACA+TGG - chr2.2:5278924-5278943 MS.gene30959:CDS 45.0%
! ACACTGTATTTTCTCTCGGG+TGG + chr2.2:5279928-5279947 None:intergenic 45.0%
! ACAGGTATTGTTCTGGACTC+TGG - chr2.2:5279376-5279395 MS.gene30959:intron 45.0%
! ATTGTTCTGGACTCTGGAGA+TGG - chr2.2:5279382-5279401 MS.gene30959:intron 45.0%
! CAACCACTGGTCTGTGATAA+TGG - chr2.2:5278387-5278406 MS.gene30959:CDS 45.0%
! CCTTGATCTTCATGCTGCTT+GGG + chr2.2:5279897-5279916 None:intergenic 45.0%
! CTTGATCTTCATGCTGCTTG+GGG + chr2.2:5279896-5279915 None:intergenic 45.0%
! TGAAGCACCTCTTAACCCAA+AGG - chr2.2:5279017-5279036 MS.gene30959:CDS 45.0%
!! CGATTAGCCTTTGGGTTAAG+AGG + chr2.2:5279027-5279046 None:intergenic 45.0%
!! CTGGTCTGTGATAATGGAAC+AGG - chr2.2:5278393-5278412 MS.gene30959:CDS 45.0%
AAGGCCGAGTACGATGAATC+CGG - chr2.2:5280137-5280156 MS.gene30959:CDS 50.0%
AGGACCGGATTCATCGTACT+CGG + chr2.2:5280144-5280163 None:intergenic 50.0%
ATGCTAGGAAACACTGCCCT+TGG + chr2.2:5278781-5278800 None:intergenic 50.0%
CCCAAGCAGCATGAAGATCA+AGG - chr2.2:5279894-5279913 MS.gene30959:intron 50.0%
CCGGTCCTTCAATTGTACAC+AGG - chr2.2:5280156-5280175 MS.gene30959:CDS 50.0%
GAAGGCTGGAAAAGAACCTC+GGG + chr2.2:5279706-5279725 None:intergenic 50.0%
GACGAAGGATAGCATGTGGA+AGG + chr2.2:5279444-5279463 None:intergenic 50.0%
GAGCAAGGAAATTACCGCGT+TGG - chr2.2:5279870-5279889 MS.gene30959:intron 50.0%
GCCCTGCTAAATCAAGACGA+AGG + chr2.2:5279459-5279478 None:intergenic 50.0%
GCTTCCATTCCAACCATGGA+AGG + chr2.2:5279724-5279743 None:intergenic 50.0%
GGAAAAGAACCTCGGGACAA+CGG + chr2.2:5279699-5279718 None:intergenic 50.0%
GGAAGGCTGGAAAAGAACCT+CGG + chr2.2:5279707-5279726 None:intergenic 50.0%
GGTGCTTCAGTGAGCAAAAC+CGG + chr2.2:5279006-5279025 None:intergenic 50.0%
GGTGGTACTACTATGTTCCC+TGG - chr2.2:5279832-5279851 MS.gene30959:intron 50.0%
GTGCTTCAGTGAGCAAAACC+GGG + chr2.2:5279005-5279024 None:intergenic 50.0%
GTTCTATCGCTCTATGCCAG+TGG - chr2.2:5279114-5279133 MS.gene30959:CDS 50.0%
TACAATTTCACGCTCGGCTG+TGG + chr2.2:5279539-5279558 None:intergenic 50.0%
TATGCCAGTGGTCGAACAAC+CGG - chr2.2:5279126-5279145 MS.gene30959:CDS 50.0%
TGCTGGAGATGATGCTCCAA+GGG - chr2.2:5278762-5278781 MS.gene30959:CDS 50.0%
TTGCTGGAGATGATGCTCCA+AGG - chr2.2:5278761-5278780 MS.gene30959:CDS 50.0%
! CTTTTCCAGCCTTCCATGGT+TGG - chr2.2:5279712-5279731 MS.gene30959:CDS 50.0%
! GGTTCTTTTCCAGCCTTCCA+TGG - chr2.2:5279708-5279727 MS.gene30959:CDS 50.0%
ACCGGTGTGATGGTTGGCAT+GGG - chr2.2:5278820-5278839 MS.gene30959:CDS 55.0%
CCAGCCTTCCATGGTTGGAA+TGG - chr2.2:5279717-5279736 MS.gene30959:CDS 55.0%
CCATTCCAACCATGGAAGGC+TGG + chr2.2:5279720-5279739 None:intergenic 55.0%
CGCCGAGGATATTCAACCAC+TGG - chr2.2:5278374-5278393 MS.gene30959:CDS 55.0%
CGTGGTCGACCAACAATGCT+AGG + chr2.2:5278796-5278815 None:intergenic 55.0%
CTAACCGGTTGTTCGACCAC+TGG + chr2.2:5279133-5279152 None:intergenic 55.0%
GATGAAGCTCAGTCCAAGCG+TGG - chr2.2:5278865-5278884 MS.gene30959:CDS 55.0%
TCCCATGCCAACCATCACAC+CGG + chr2.2:5278824-5278843 None:intergenic 55.0%
AGCAAAACCGGGTGCTCCTC+TGG + chr2.2:5278994-5279013 None:intergenic 60.0%
CAAAACCGGGTGCTCCTCTG+GGG + chr2.2:5278992-5279011 None:intergenic 60.0%
CACCGGTGTGATGGTTGGCA+TGG - chr2.2:5278819-5278838 MS.gene30959:CDS 60.0%
GCAAAACCGGGTGCTCCTCT+GGG + chr2.2:5278993-5279012 None:intergenic 60.0%
TGAGCGTTTCCGTTGTCCCG+AGG - chr2.2:5279687-5279706 MS.gene30959:CDS 60.0%
TGCCAACCATCACACCGGTG+TGG + chr2.2:5278819-5278838 None:intergenic 60.0%
ACCACGCCACACCGGTGTGA+TGG - chr2.2:5278810-5278829 MS.gene30959:CDS 65.0%
ACCATCACACCGGTGTGGCG+TGG + chr2.2:5278814-5278833 None:intergenic 65.0%
CGCCACACCGGTGTGATGGT+TGG - chr2.2:5278814-5278833 MS.gene30959:CDS 65.0%
GTTGGTCGACCACGCCACAC+CGG - chr2.2:5278802-5278821 MS.gene30959:CDS 65.0%
TGAACTCCGTGTGGCCCCAG+AGG - chr2.2:5278975-5278994 MS.gene30959:CDS 65.0%
! CATGCTGCTTGGGGCCAACG+CGG + chr2.2:5279887-5279906 None:intergenic 65.0%
!! GGTGCTCCTCTGGGGCCACA+CGG + chr2.2:5278984-5279003 None:intergenic 70.0%
!! TGTGGCCCCAGAGGAGCACC+CGG - chr2.2:5278984-5279003 MS.gene30959:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 5278366 5280190 5278366 ID=MS.gene30959
chr2.2 mRNA 5278366 5280190 5278366 ID=MS.gene30959.t1;Parent=MS.gene30959
chr2.2 exon 5280131 5280190 5280131 ID=MS.gene30959.t1.exon1;Parent=MS.gene30959.t1
chr2.2 CDS 5280131 5280190 5280131 ID=cds.MS.gene30959.t1;Parent=MS.gene30959.t1
chr2.2 exon 5279409 5279802 5279409 ID=MS.gene30959.t1.exon2;Parent=MS.gene30959.t1
chr2.2 CDS 5279409 5279802 5279409 ID=cds.MS.gene30959.t1;Parent=MS.gene30959.t1
chr2.2 exon 5278563 5279176 5278563 ID=MS.gene30959.t1.exon3;Parent=MS.gene30959.t1
chr2.2 CDS 5278563 5279176 5278563 ID=cds.MS.gene30959.t1;Parent=MS.gene30959.t1
chr2.2 exon 5278366 5278431 5278366 ID=MS.gene30959.t1.exon4;Parent=MS.gene30959.t1
chr2.2 CDS 5278366 5278431 5278366 ID=cds.MS.gene30959.t1;Parent=MS.gene30959.t1
Gene Sequence

>MS.gene30959

ATGGCAGACGCCGAGGATATTCAACCACTGGTCTGTGATAATGGAACAGGAATGGTTAAGGTAAAGCACAAATGTGATTCTAGTTTCACAATTTTTCCTGAAGAAGCGATTTGGCTATGTATCTTTTCTGTTTTCTCTTCGGTGTTGATAAATAATCGTTATAGCAATGTAGCGAAATTTGAACAAATCAATATTGTTTTGTGATACACTATCTAGTACTAAATGTTGTTGTTAAGGAGCTGCTATAAGTCAATAGCACTATTGCATAGCAGAATTTGAATGAACTGTTCTTTTCTACGATCTGCGATTGGCAACATTGTTTTTGTTTGTTTGATACCTTTCTACTAATTTGCTGTGAAACTATTGCATTGTGATTTGCAACATTTAGGCTGGATTTGCTGGAGATGATGCTCCAAGGGCAGTGTTTCCTAGCATTGTTGGTCGACCACGCCACACCGGTGTGATGGTTGGCATGGGACAAAAAGATGCATATGTTGGTGATGAAGCTCAGTCCAAGCGTGGTATATTGACTCTAAAATATCCAATTGAGCATGGTATTGTGAGTAACTGGGATGACATGGAAAAGATTTGGCATCATACCTTTTACAATGAACTCCGTGTGGCCCCAGAGGAGCACCCGGTTTTGCTCACTGAAGCACCTCTTAACCCAAAGGCTAATCGTGAGAAAATGACACAAATTATGTTTGAGACATTCAACACTCCTGCTATGTATGTTGCTATTCAGGCCGTTCTATCGCTCTATGCCAGTGGTCGAACAACCGGTTAGTCATTTCCCGATTTAAGAAAACTAATGTGTCGCGGCTAGTCTAGTTTACATTCTAAGTTTTTTTATACTGGCCAGTAAAAATAAGCATGCATTGCAAGGTTCAGTTTGCTAATGGATGGAAAAAGAGGGAAAATAGACAAAAATGATTTCAAAGAATTTGGCTCTATATTTATGGCTATTTTATTATGATTAAATTCTGATTGAAAACTCTATATTAATATGTACAGGTATTGTTCTGGACTCTGGAGATGGTGTCAGCCACACTGTACCTATCTATGAAGGATATGCCCTTCCACATGCTATCCTTCGTCTTGATTTAGCAGGGCGTGACCTCACTGATGCCTTGATGAAAATCCTTACTGAGCGTGGTTATTCTTTCACTACCACAGCCGAGCGTGAAATTGTAAGAGATGTGAAGGAAAAGCTGTCTTATATTGCCCTTGATTTTGAGCAAGAGCTTGAAACTTCAAAAACAAGCTCTGCTGTTGAGAAGAGCTATGAGCTTCCTGATGGACAGATCATCACTATTGGTGCTGAGCGTTTCCGTTGTCCCGAGGTTCTTTTCCAGCCTTCCATGGTTGGAATGGAAGCTGTTGGCATTCATGAGACGACATATAACTCAATCATGAAGTGCGATGTTGATATAAGAAAGGACCTGTATGGAAACATTGTCCTTAGTGGTGGTACTACTATGTTCCCTGGTATTGCTGACAGAATGAGCAAGGAAATTACCGCGTTGGCCCCAAGCAGCATGAAGATCAAGGTGGTTGCTCCACCCGAGAGAAAATACAGTGTCTGGATTGGTGGCTCCATCTTAGCTTCTCTTAGCACTTTTCAACAGGTGAGAAACCGATATATTCACATTGTCCGTGTGTATTTATGCACGCCTTAGAATTCTTTTTTTTCTTCTTACATGTTTGATTTATTTGGGATACTAATGAATGATGAAACACTTATTTTTTGAAAATGCAGATGTGGATTGCAAAGGCCGAGTACGATGAATCCGGTCCTTCAATTGTACACAGGAAATGCTTTTAG
Protein sequence

>MS.gene30959.t1

MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDVKEKLSYIALDFEQELETSKTSSAVEKSYELPDGQIITIGAERFRCPEVLFQPSMVGMEAVGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF