Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052120.t1 | XP_013465392.1 | 100 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 1.40E-216 | 761.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052120.t1 | O81221 | 97.3 | 377 | 10 | 0 | 1 | 377 | 1 | 377 | 5.9e-216 | 751.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052120.t1 | A0A072VCE0 | 100.0 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 1.0e-216 | 761.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052120 | MS.gene31154 | 0.970152 | 3.48E-131 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052120.t1 | MTR_2g096840 | 100.000 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 0.0 | 784 |
| MS.gene052120.t1 | MTR_0015s0130 | 98.674 | 377 | 5 | 0 | 1 | 377 | 1 | 377 | 0.0 | 776 |
| MS.gene052120.t1 | MTR_7g026230 | 97.082 | 377 | 11 | 0 | 1 | 377 | 1 | 377 | 0.0 | 768 |
| MS.gene052120.t1 | MTR_6g004000 | 97.082 | 377 | 11 | 0 | 1 | 377 | 1 | 377 | 0.0 | 768 |
| MS.gene052120.t1 | MTR_7g093260 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 765 |
| MS.gene052120.t1 | MTR_3g095530 | 94.960 | 377 | 19 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene052120.t1 | MTR_2g008050 | 93.634 | 377 | 24 | 0 | 1 | 377 | 1 | 377 | 0.0 | 752 |
| MS.gene052120.t1 | MTR_7g027185 | 42.480 | 379 | 204 | 3 | 10 | 374 | 7 | 385 | 1.41e-118 | 349 |
| MS.gene052120.t1 | MTR_7g027185 | 43.662 | 355 | 187 | 2 | 10 | 351 | 7 | 361 | 7.33e-116 | 341 |
| MS.gene052120.t1 | MTR_7g027185 | 46.580 | 307 | 162 | 1 | 10 | 314 | 7 | 313 | 2.75e-108 | 320 |
| MS.gene052120.t1 | MTR_5g066400 | 37.360 | 447 | 206 | 11 | 1 | 376 | 1 | 444 | 4.42e-93 | 286 |
| MS.gene052120.t1 | MTR_8g464900 | 36.574 | 432 | 201 | 10 | 1 | 362 | 1 | 429 | 1.71e-86 | 268 |
| MS.gene052120.t1 | MTR_5g066400 | 34.753 | 446 | 191 | 11 | 1 | 376 | 1 | 416 | 2.52e-80 | 252 |
| MS.gene052120.t1 | MTR_8g089630 | 34.550 | 411 | 219 | 8 | 10 | 372 | 10 | 418 | 5.49e-72 | 231 |
| MS.gene052120.t1 | MTR_5g066400 | 34.250 | 400 | 192 | 10 | 1 | 332 | 1 | 397 | 4.11e-69 | 223 |
| MS.gene052120.t1 | MTR_3g030550 | 37.105 | 380 | 213 | 9 | 7 | 377 | 1 | 363 | 1.07e-67 | 218 |
| MS.gene052120.t1 | MTR_2g445210 | 28.132 | 423 | 238 | 7 | 8 | 366 | 6 | 426 | 3.20e-55 | 188 |
| MS.gene052120.t1 | MTR_8g007440 | 35.227 | 264 | 144 | 7 | 77 | 319 | 9 | 266 | 3.45e-46 | 159 |
| MS.gene052120.t1 | MTR_2g445210 | 26.613 | 372 | 207 | 7 | 8 | 315 | 6 | 375 | 3.38e-41 | 149 |
| MS.gene052120.t1 | MTR_3g010170 | 30.303 | 297 | 183 | 9 | 91 | 371 | 179 | 467 | 2.14e-40 | 149 |
| MS.gene052120.t1 | MTR_8g070520 | 31.058 | 293 | 175 | 10 | 91 | 366 | 187 | 469 | 7.96e-36 | 136 |
| MS.gene052120.t1 | MTR_8g070520 | 30.576 | 278 | 167 | 9 | 91 | 352 | 187 | 454 | 1.62e-32 | 127 |
| MS.gene052120.t1 | MTR_3g067945 | 28.968 | 252 | 160 | 7 | 9 | 245 | 21 | 268 | 9.70e-22 | 97.4 |
| MS.gene052120.t1 | MTR_3g067945 | 33.607 | 122 | 78 | 2 | 250 | 368 | 586 | 707 | 4.69e-16 | 80.1 |
| MS.gene052120.t1 | MTR_8g008540 | 70.270 | 37 | 11 | 0 | 340 | 376 | 41 | 77 | 4.15e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052120.t1 | AT3G12110 | 96.552 | 377 | 13 | 0 | 1 | 377 | 1 | 377 | 0.0 | 765 |
| MS.gene052120.t1 | AT3G53750 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene052120.t1 | AT3G53750 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene052120.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene052120.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene052120.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene052120.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene052120.t1 | AT5G59370 | 96.817 | 377 | 12 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MS.gene052120.t1 | AT5G59370 | 96.817 | 377 | 12 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MS.gene052120.t1 | AT3G46520 | 96.552 | 377 | 13 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene052120.t1 | AT3G46520 | 96.552 | 377 | 13 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene052120.t1 | AT5G09810 | 94.960 | 377 | 19 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene052120.t1 | AT1G49240 | 92.573 | 377 | 28 | 0 | 1 | 377 | 1 | 377 | 0.0 | 745 |
| MS.gene052120.t1 | AT3G18780 | 92.308 | 377 | 29 | 0 | 1 | 377 | 1 | 377 | 0.0 | 744 |
| MS.gene052120.t1 | AT3G18780 | 92.308 | 377 | 29 | 0 | 1 | 377 | 1 | 377 | 0.0 | 744 |
| MS.gene052120.t1 | AT3G18780 | 92.201 | 359 | 28 | 0 | 1 | 359 | 1 | 359 | 0.0 | 703 |
| MS.gene052120.t1 | AT2G42100 | 83.967 | 368 | 59 | 0 | 10 | 377 | 11 | 378 | 0.0 | 673 |
| MS.gene052120.t1 | AT2G42090 | 70.000 | 370 | 106 | 1 | 7 | 376 | 1 | 365 | 0.0 | 564 |
| MS.gene052120.t1 | AT2G42170 | 77.108 | 332 | 73 | 1 | 46 | 377 | 1 | 329 | 0.0 | 556 |
| MS.gene052120.t1 | AT2G42170 | 77.108 | 332 | 73 | 1 | 46 | 377 | 1 | 329 | 0.0 | 556 |
| MS.gene052120.t1 | AT2G42170 | 76.923 | 312 | 69 | 1 | 46 | 357 | 1 | 309 | 0.0 | 518 |
| MS.gene052120.t1 | AT2G42170 | 76.923 | 312 | 69 | 1 | 46 | 357 | 1 | 309 | 0.0 | 518 |
| MS.gene052120.t1 | AT2G42170 | 76.991 | 226 | 49 | 1 | 152 | 377 | 9 | 231 | 2.18e-130 | 373 |
| MS.gene052120.t1 | AT3G27000 | 43.536 | 379 | 200 | 4 | 10 | 374 | 7 | 385 | 4.89e-121 | 355 |
| MS.gene052120.t1 | AT1G18450 | 37.472 | 443 | 207 | 9 | 1 | 376 | 1 | 440 | 1.50e-95 | 292 |
| MS.gene052120.t1 | AT1G13180 | 34.223 | 412 | 219 | 9 | 10 | 372 | 9 | 417 | 2.76e-72 | 232 |
| MS.gene052120.t1 | AT3G60830 | 37.467 | 379 | 213 | 9 | 7 | 377 | 1 | 363 | 5.82e-67 | 216 |
| MS.gene052120.t1 | AT3G33520 | 30.166 | 421 | 236 | 7 | 10 | 377 | 5 | 420 | 5.44e-58 | 194 |
| MS.gene052120.t1 | AT5G56180 | 31.338 | 284 | 170 | 9 | 91 | 358 | 50 | 324 | 1.95e-35 | 132 |
| MS.gene052120.t1 | AT5G56180 | 31.338 | 284 | 170 | 9 | 91 | 358 | 182 | 456 | 1.19e-34 | 133 |
| MS.gene052120.t1 | AT3G12380 | 29.183 | 257 | 159 | 6 | 9 | 245 | 21 | 274 | 1.21e-24 | 106 |
| MS.gene052120.t1 | AT3G12380 | 32.738 | 168 | 106 | 4 | 207 | 368 | 550 | 716 | 3.86e-17 | 83.6 |
| MS.gene052120.t1 | AT3G12380 | 29.020 | 255 | 156 | 6 | 9 | 245 | 21 | 268 | 4.83e-24 | 104 |
| MS.gene052120.t1 | AT3G12380 | 32.738 | 168 | 106 | 4 | 207 | 368 | 544 | 710 | 3.74e-17 | 83.6 |
| MS.gene052120.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.08e-23 | 95.1 |
| MS.gene052120.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.08e-23 | 95.1 |
| MS.gene052120.t1 | AT5G56180 | 32.178 | 202 | 114 | 8 | 91 | 281 | 182 | 371 | 2.08e-19 | 89.4 |
| MS.gene052120.t1 | AT1G73910 | 29.268 | 123 | 71 | 2 | 1 | 107 | 1 | 123 | 3.00e-12 | 64.7 |
Find 102 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGATCTTCATGCTGCTT+GGG | 0.250595 | 2.3:+5821271 | None:intergenic |
| AGCAAAACCGGGTGCTCCTC+TGG | 0.272892 | 2.3:+5822219 | None:intergenic |
| CAACCACTGGTCTGTGATAA+TGG | 0.278942 | 2.3:-5822829 | MS.gene052120:CDS |
| AACATTTAGGCTGGATTTGC+TGG | 0.289528 | 2.3:-5822465 | MS.gene052120:intron |
| TGCTATGTATGTTGCTATTC+AGG | 0.305017 | 2.3:-5822121 | MS.gene052120:CDS |
| GGGATGACATGGAGAAAATT+TGG | 0.308086 | 2.3:-5822275 | MS.gene052120:CDS |
| AGGGCATATCCTTCATAAAT+AGG | 0.317673 | 2.3:+5821744 | None:intergenic |
| CACACGGAGTTCATTGTAAA+AGG | 0.330285 | 2.3:+5822245 | None:intergenic |
| ACCTTGATCTTCATGCTGCT+TGG | 0.336588 | 2.3:+5821270 | None:intergenic |
| AGCATGGTATTGTGAGTAAC+TGG | 0.340011 | 2.3:-5822296 | MS.gene052120:CDS |
| GGTGCTTCAGTGAGCAAAAC+CGG | 0.396077 | 2.3:+5822207 | None:intergenic |
| CTCACAATACCATGCTCAAT+TGG | 0.397001 | 2.3:+5822303 | None:intergenic |
| CAAGCTCTTGCTCAAAATCA+AGG | 0.399460 | 2.3:+5821574 | None:intergenic |
| CTCATTCTGTCAGCAATGCC+AGG | 0.400398 | 2.3:+5821315 | None:intergenic |
| ATCCTTCGTCTTGATTTAGC+AGG | 0.412905 | 2.3:-5821711 | MS.gene052120:CDS |
| GATGGCCAGATCATCACTAT+TGG | 0.420978 | 2.3:-5821504 | MS.gene052120:CDS |
| CCGAGAGAAAATACAGTGTC+TGG | 0.425088 | 2.3:-5821236 | MS.gene052120:CDS |
| GCAAAACCGGGTGCTCCTCT+GGG | 0.429592 | 2.3:+5822220 | None:intergenic |
| TACAATTTCACGCTCGGCTG+TGG | 0.436320 | 2.3:+5821629 | None:intergenic |
| TATGGAAATATTGTCCTTAG+TGG | 0.444109 | 2.3:-5821354 | MS.gene052120:CDS |
| CATTTCCTGTGTACAATTGA+AGG | 0.448729 | 2.3:+5821011 | None:intergenic |
| CACTGGTGTGATGGTTGGCA+TGG | 0.449957 | 2.3:-5822391 | MS.gene052120:CDS |
| GATATCAGAAAGGACTTGTA+TGG | 0.450042 | 2.3:-5821372 | MS.gene052120:CDS |
| AGTGCTAAGAGAAGCTAAGA+TGG | 0.454030 | 2.3:+5821203 | None:intergenic |
| CTTGATCTTCATGCTGCTTG+GGG | 0.455780 | 2.3:+5821272 | None:intergenic |
| TATGCTAGTGGTCGAACAAC+CGG | 0.457727 | 2.3:-5822084 | MS.gene052120:intron |
| CAGCTCCAATAGTGATGATC+TGG | 0.458059 | 2.3:+5821499 | None:intergenic |
| ATCTCTTACAATTTCACGCT+CGG | 0.471407 | 2.3:+5821623 | None:intergenic |
| GCTGTGTTTCCTAGCATTGT+TGG | 0.477335 | 2.3:-5822426 | MS.gene052120:CDS |
| TAATGGAACAGGAATGGTTA+AGG | 0.478719 | 2.3:-5822812 | MS.gene052120:intron |
| CTGGTCTGTGATAATGGAAC+AGG | 0.481376 | 2.3:-5822823 | MS.gene052120:CDS |
| AAATACAGTGTCTGGATTGG+TGG | 0.485676 | 2.3:-5821228 | MS.gene052120:CDS |
| CCAGCCTTCCATGGTTGGAA+TGG | 0.489084 | 2.3:-5821448 | MS.gene052120:CDS |
| ATAGTGATGATCTGGCCATC+AGG | 0.494142 | 2.3:+5821507 | None:intergenic |
| AAGGCCGAGTACGATGAATC+TGG | 0.498285 | 2.3:-5821035 | MS.gene052120:CDS |
| AAGAATAACCACGCTCGGTA+AGG | 0.505013 | 2.3:+5821658 | None:intergenic |
| ATGAAAATCCTTACCGAGCG+TGG | 0.509930 | 2.3:-5821666 | MS.gene052120:CDS |
| AGAAAATACAGTGTCTGGAT+TGG | 0.510090 | 2.3:-5821231 | MS.gene052120:CDS |
| CCATTCCAACCATGGAAGGC+TGG | 0.515654 | 2.3:+5821448 | None:intergenic |
| TAGAGTCAATATACCACGCT+TGG | 0.516667 | 2.3:+5822332 | None:intergenic |
| CACACTGTGCCTATTTATGA+AGG | 0.522782 | 2.3:-5821753 | MS.gene052120:CDS |
| ATGCTAGGAAACACAGCCCT+TGG | 0.526579 | 2.3:+5822432 | None:intergenic |
| TGCTGGAGATGATGCTCCAA+GGG | 0.531721 | 2.3:-5822448 | MS.gene052120:CDS |
| GGTGGTACTACTATGTTCCC+TGG | 0.534257 | 2.3:-5821333 | MS.gene052120:CDS |
| GCAGGTATTGTACTGGACTC+TGG | 0.536986 | 2.3:-5821789 | MS.gene052120:intron |
| CGATTAGCCTTTGGATTGAG+CGG | 0.541575 | 2.3:+5822186 | None:intergenic |
| GACCAGTGGTTGAATATCCT+CGG | 0.542292 | 2.3:+5822840 | None:intergenic |
| CGCCGAGGATATTCAACCAC+TGG | 0.542623 | 2.3:-5822842 | MS.gene052120:CDS |
| CCCAAGCAGCATGAAGATCA+AGG | 0.543751 | 2.3:-5821271 | MS.gene052120:CDS |
| AATATGTGCAGGTATTGTAC+TGG | 0.544002 | 2.3:-5821796 | MS.gene052120:intron |
| TCCTTCGTCTTGATTTAGCA+GGG | 0.546953 | 2.3:-5821710 | MS.gene052120:CDS |
| GAAGGCTGGAAAAGAACCTC+AGG | 0.547727 | 2.3:+5821462 | None:intergenic |
| TGAAATTGTAAGAGATGTGA+AGG | 0.550481 | 2.3:-5821616 | MS.gene052120:CDS |
| CGTGGTCGACCAACAATGCT+AGG | 0.552232 | 2.3:+5822417 | None:intergenic |
| ACATAGTAGTACCACCACTA+AGG | 0.559599 | 2.3:+5821340 | None:intergenic |
| TTGCTGGAGATGATGCTCCA+AGG | 0.560432 | 2.3:-5822449 | MS.gene052120:CDS |
| GACGAAGGATAGCATGTGGA+AGG | 0.560879 | 2.3:+5821724 | None:intergenic |
| AAGCTCTTGCTCAAAATCAA+GGG | 0.566750 | 2.3:+5821575 | None:intergenic |
| ACCACGCCACACTGGTGTGA+TGG | 0.574183 | 2.3:-5822400 | MS.gene052120:CDS |
| CTAAAATATCCAATTGAGCA+TGG | 0.574515 | 2.3:-5822312 | MS.gene052120:CDS |
| TGTGGCCCCAGAGGAGCACC+CGG | 0.576326 | 2.3:-5822226 | MS.gene052120:CDS |
| TGAATAGCAACATACATAGC+AGG | 0.576757 | 2.3:+5822123 | None:intergenic |
| CGCCACACTGGTGTGATGGT+TGG | 0.577341 | 2.3:-5822396 | MS.gene052120:CDS |
| GTGCTTCAGTGAGCAAAACC+GGG | 0.578129 | 2.3:+5822208 | None:intergenic |
| GCTTCCATTCCAACCATGGA+AGG | 0.580286 | 2.3:+5821444 | None:intergenic |
| GGTGCTCCTCTGGGGCCACA+CGG | 0.580962 | 2.3:+5822229 | None:intergenic |
| AACAGCTTCCATTCCAACCA+TGG | 0.591828 | 2.3:+5821440 | None:intergenic |
| GAGCAAGGAAATTACCGCGT+TGG | 0.592403 | 2.3:-5821295 | MS.gene052120:CDS |
| GGAAATATTGTCCTTAGTGG+TGG | 0.594437 | 2.3:-5821351 | MS.gene052120:CDS |
| AGTGAAAGAATAACCACGCT+CGG | 0.601145 | 2.3:+5821653 | None:intergenic |
| AGGACCAGATTCATCGTACT+CGG | 0.601521 | 2.3:+5821031 | None:intergenic |
| TGTGAGTAACTGGGATGACA+TGG | 0.601686 | 2.3:-5822286 | MS.gene052120:CDS |
| TGAGCGTTTCCGTTGTCCTG+AGG | 0.602279 | 2.3:-5821478 | MS.gene052120:CDS |
| ATTGTACTGGACTCTGGAGA+TGG | 0.605459 | 2.3:-5821783 | MS.gene052120:CDS |
| GTTGGTCGACCACGCCACAC+TGG | 0.610992 | 2.3:-5822408 | MS.gene052120:CDS |
| ATGGTTGGAATGGAAGCTGT+TGG | 0.612805 | 2.3:-5821438 | MS.gene052120:CDS |
| TGAAGCACCGCTCAATCCAA+AGG | 0.625231 | 2.3:-5822193 | MS.gene052120:CDS |
| CATGCTGCTTGGGGCCAACG+CGG | 0.625414 | 2.3:+5821281 | None:intergenic |
| CATTGCTGACAGAATGAGCA+AGG | 0.625589 | 2.3:-5821310 | MS.gene052120:CDS |
| ACCATCACACCAGTGTGGCG+TGG | 0.628231 | 2.3:+5822399 | None:intergenic |
| CTGTGATAATGGAACAGGAA+TGG | 0.632340 | 2.3:-5822818 | MS.gene052120:CDS |
| CTGGTCCTTCAATTGTACAC+AGG | 0.634534 | 2.3:-5821016 | MS.gene052120:CDS |
| ACTAGCATAGAGCGATAGAA+CGG | 0.636987 | 2.3:+5822098 | None:intergenic |
| CAAAACCGGGTGCTCCTCTG+GGG | 0.638105 | 2.3:+5822221 | None:intergenic |
| GTTCTATCGCTCTATGCTAG+TGG | 0.639915 | 2.3:-5822096 | MS.gene052120:CDS |
| TCAAGACGAAGGATAGCATG+TGG | 0.640472 | 2.3:+5821720 | None:intergenic |
| AAGAGTTATGAACTTCCTGA+TGG | 0.641331 | 2.3:-5821522 | MS.gene052120:CDS |
| TCATTCTGTCAGCAATGCCA+GGG | 0.641800 | 2.3:+5821316 | None:intergenic |
| ACTGGTGTGATGGTTGGCAT+GGG | 0.642331 | 2.3:-5822390 | MS.gene052120:CDS |
| TGAACTCCGTGTGGCCCCAG+AGG | 0.648993 | 2.3:-5822235 | MS.gene052120:CDS |
| AAAGACAATGGCAGACGCCG+AGG | 0.653794 | 2.3:-5822857 | None:intergenic |
| GGAAAAGAACCTCAGGACAA+CGG | 0.659232 | 2.3:+5821469 | None:intergenic |
| ACGAAGGATAGCATGTGGAA+GGG | 0.659324 | 2.3:+5821725 | None:intergenic |
| GATGAAGCTCAGTCCAAGCG+TGG | 0.663966 | 2.3:-5822345 | MS.gene052120:CDS |
| AATGCAGATGTGGATTGCAA+AGG | 0.664746 | 2.3:-5821054 | MS.gene052120:intron |
| GTGTGATGTTGATATCAGAA+AGG | 0.677167 | 2.3:-5821382 | MS.gene052120:CDS |
| GTTCCATTATCACAGACCAG+TGG | 0.693343 | 2.3:+5822826 | None:intergenic |
| TGCCAACCATCACACCAGTG+TGG | 0.706399 | 2.3:+5822394 | None:intergenic |
| GCATGGTATTGTGAGTAACT+GGG | 0.709006 | 2.3:-5822295 | MS.gene052120:CDS |
| GCCCTGCTAAATCAAGACGA+AGG | 0.714666 | 2.3:+5821709 | None:intergenic |
| CTTCATAAATAGGCACAGTG+TGG | 0.723850 | 2.3:+5821754 | None:intergenic |
| AAGCAGCATGAAGATCAAGG+TGG | 0.767710 | 2.3:-5821268 | MS.gene052120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATTGTTCTATATCTA+TGG | - | chr2.3:5821997-5822016 | MS.gene052120:intron | 15.0% |
| !! | ATGTCAAAAAAAGAATTCTA+AGG | + | chr2.3:5822731-5822750 | None:intergenic | 20.0% |
| !!! | CATTCTAAGTTTTTTTATAC+TGG | - | chr2.3:5821844-5821863 | MS.gene052120:intron | 20.0% |
| !!! | TTGACATGTTTGATTTATTT+GGG | - | chr2.3:5822744-5822763 | MS.gene052120:intron | 20.0% |
| !!! | TTTGACATGTTTGATTTATT+TGG | - | chr2.3:5822743-5822762 | MS.gene052120:intron | 20.0% |
| !! | ACACATTAGTTTCTTAAATC+GGG | + | chr2.3:5821806-5821825 | None:intergenic | 25.0% |
| !! | AGTACTAAATGTTGTTGTTA+AGG | - | chr2.3:5821218-5821237 | MS.gene052120:CDS | 25.0% |
| !! | GACACATTAGTTTCTTAAAT+CGG | + | chr2.3:5821807-5821826 | None:intergenic | 25.0% |
| !! | TATCTTTTCTGTTTTCTCTT+CGG | - | chr2.3:5821123-5821142 | MS.gene052120:intron | 25.0% |
| !!! | TATTTTTGAAAATGCAGATG+TGG | - | chr2.3:5822790-5822809 | MS.gene052120:intron | 25.0% |
| !!! | TCAGTTTGTTAATGGATATA+TGG | - | chr2.3:5821896-5821915 | MS.gene052120:intron | 25.0% |
| AAAAATAAGAATGCGTTGCA+AGG | - | chr2.3:5821872-5821891 | MS.gene052120:intron | 30.0% | |
| AAGGGGAAAATAGACAAAAA+TGG | - | chr2.3:5821967-5821986 | MS.gene052120:intron | 30.0% | |
| AGTTAAGTTCATGGGAAAAA+GGG | - | chr2.3:5821949-5821968 | MS.gene052120:intron | 30.0% | |
| ATACCTGCACATATTCATAT+AGG | + | chr2.3:5822053-5822072 | None:intergenic | 30.0% | |
| ATGAAGTCTAGTTAAGTTCA+TGG | - | chr2.3:5821940-5821959 | MS.gene052120:intron | 30.0% | |
| CTAAAATATCCAATTGAGCA+TGG | - | chr2.3:5821542-5821561 | MS.gene052120:CDS | 30.0% | |
| TACCTGCACATATTCATATA+GGG | + | chr2.3:5822052-5822071 | None:intergenic | 30.0% | |
| TAGTTAAGTTCATGGGAAAA+AGG | - | chr2.3:5821948-5821967 | MS.gene052120:intron | 30.0% | |
| TATGGAAATATTGTCCTTAG+TGG | - | chr2.3:5822500-5822519 | MS.gene052120:intron | 30.0% | |
| TCACAGCAAATTAGTAGAAA+GGG | + | chr2.3:5821348-5821367 | None:intergenic | 30.0% | |
| TGAAATTGTAAGAGATGTGA+AGG | - | chr2.3:5822238-5822257 | MS.gene052120:CDS | 30.0% | |
| TGAAGTCTAGTTAAGTTCAT+GGG | - | chr2.3:5821941-5821960 | MS.gene052120:intron | 30.0% | |
| TTCACAGCAAATTAGTAGAA+AGG | + | chr2.3:5821349-5821368 | None:intergenic | 30.0% | |
| ! | CATTGTGATTTGCAACATTT+AGG | - | chr2.3:5821376-5821395 | MS.gene052120:CDS | 30.0% |
| AACCCTATATGAATATGTGC+AGG | - | chr2.3:5822047-5822066 | MS.gene052120:intron | 35.0% | |
| AAGAGTTATGAACTTCCTGA+TGG | - | chr2.3:5822332-5822351 | MS.gene052120:CDS | 35.0% | |
| AAGCTCTTGCTCAAAATCAA+GGG | + | chr2.3:5822282-5822301 | None:intergenic | 35.0% | |
| AGAAAATACAGTGTCTGGAT+TGG | - | chr2.3:5822623-5822642 | MS.gene052120:intron | 35.0% | |
| AGGGCATATCCTTCATAAAT+AGG | + | chr2.3:5822113-5822132 | None:intergenic | 35.0% | |
| ATCTCTTACAATTTCACGCT+CGG | + | chr2.3:5822234-5822253 | None:intergenic | 35.0% | |
| CATTTCCTGTGTACAATTGA+AGG | + | chr2.3:5822846-5822865 | None:intergenic | 35.0% | |
| GATATCAGAAAGGACTTGTA+TGG | - | chr2.3:5822482-5822501 | MS.gene052120:intron | 35.0% | |
| GGACAAAAAGATGCATATGT+TGG | - | chr2.3:5821485-5821504 | MS.gene052120:CDS | 35.0% | |
| GTGTGATGTTGATATCAGAA+AGG | - | chr2.3:5822472-5822491 | MS.gene052120:intron | 35.0% | |
| GTTAAGTTCATGGGAAAAAG+GGG | - | chr2.3:5821950-5821969 | MS.gene052120:intron | 35.0% | |
| TAAATCGGGAAATGACTAAC+CGG | + | chr2.3:5821792-5821811 | None:intergenic | 35.0% | |
| TAATGGAACAGGAATGGTTA+AGG | - | chr2.3:5821042-5821061 | MS.gene052120:CDS | 35.0% | |
| TGAATAGCAACATACATAGC+AGG | + | chr2.3:5821734-5821753 | None:intergenic | 35.0% | |
| ! | AATATGTGCAGGTATTGTAC+TGG | - | chr2.3:5822058-5822077 | MS.gene052120:intron | 35.0% |
| ! | TGCTATGTATGTTGCTATTC+AGG | - | chr2.3:5821733-5821752 | MS.gene052120:CDS | 35.0% |
| ! | TTCTCTTAGCACTTTTCAAC+AGG | - | chr2.3:5822661-5822680 | MS.gene052120:intron | 35.0% |
| ! | TTTTTCCTGAAGAAGCGATT+TGG | - | chr2.3:5821095-5821114 | MS.gene052120:intron | 35.0% |
| !! | TGCAAGGTTCAGTTTGTTAA+TGG | - | chr2.3:5821888-5821907 | MS.gene052120:intron | 35.0% |
| !!! | ATTTTCTCACGATTAGCCTT+TGG | + | chr2.3:5821680-5821699 | None:intergenic | 35.0% |
| AACATTTAGGCTGGATTTGC+TGG | - | chr2.3:5821389-5821408 | MS.gene052120:CDS | 40.0% | |
| AAGGCATGCATAAATACACG+CGG | + | chr2.3:5822712-5822731 | None:intergenic | 40.0% | |
| ACATAGTAGTACCACCACTA+AGG | + | chr2.3:5822517-5822536 | None:intergenic | 40.0% | |
| ACTAGCATAGAGCGATAGAA+CGG | + | chr2.3:5821759-5821778 | None:intergenic | 40.0% | |
| AGCATGGTATTGTGAGTAAC+TGG | - | chr2.3:5821558-5821577 | MS.gene052120:CDS | 40.0% | |
| AGTGAAAGAATAACCACGCT+CGG | + | chr2.3:5822204-5822223 | None:intergenic | 40.0% | |
| AGTGCTAAGAGAAGCTAAGA+TGG | + | chr2.3:5822654-5822673 | None:intergenic | 40.0% | |
| ATCCTTCGTCTTGATTTAGC+AGG | - | chr2.3:5822143-5822162 | MS.gene052120:CDS | 40.0% | |
| CAAGCTCTTGCTCAAAATCA+AGG | + | chr2.3:5822283-5822302 | None:intergenic | 40.0% | |
| CACACGGAGTTCATTGTAAA+AGG | + | chr2.3:5821612-5821631 | None:intergenic | 40.0% | |
| CACACTGTGCCTATTTATGA+AGG | - | chr2.3:5822101-5822120 | MS.gene052120:CDS | 40.0% | |
| CTCACAATACCATGCTCAAT+TGG | + | chr2.3:5821554-5821573 | None:intergenic | 40.0% | |
| CTTCATAAATAGGCACAGTG+TGG | + | chr2.3:5822103-5822122 | None:intergenic | 40.0% | |
| GCATGGTATTGTGAGTAACT+GGG | - | chr2.3:5821559-5821578 | MS.gene052120:CDS | 40.0% | |
| GGAAATATTGTCCTTAGTGG+TGG | - | chr2.3:5822503-5822522 | MS.gene052120:intron | 40.0% | |
| GGGATGACATGGAGAAAATT+TGG | - | chr2.3:5821579-5821598 | MS.gene052120:CDS | 40.0% | |
| TAGAGTCAATATACCACGCT+TGG | + | chr2.3:5821525-5821544 | None:intergenic | 40.0% | |
| TCCTTCGTCTTGATTTAGCA+GGG | - | chr2.3:5822144-5822163 | MS.gene052120:CDS | 40.0% | |
| ! | AAATACAGTGTCTGGATTGG+TGG | - | chr2.3:5822626-5822645 | MS.gene052120:intron | 40.0% |
| ! | AATGCAGATGTGGATTGCAA+AGG | - | chr2.3:5822800-5822819 | MS.gene052120:intron | 40.0% |
| ! | CAGACACTGTATTTTCTCTC+GGG | + | chr2.3:5822620-5822639 | None:intergenic | 40.0% |
| ! | CCAGACACTGTATTTTCTCT+CGG | + | chr2.3:5822621-5822640 | None:intergenic | 40.0% |
| ! | CTCGGTAAGGATTTTCATCA+AGG | + | chr2.3:5822186-5822205 | None:intergenic | 40.0% |
| ! | CTTTTACAATGAACTCCGTG+TGG | - | chr2.3:5821610-5821629 | MS.gene052120:CDS | 40.0% |
| ! | GTGATTTGCAACATTTAGGC+TGG | - | chr2.3:5821380-5821399 | MS.gene052120:CDS | 40.0% |
| ! | TCTTTTCTACGATCTGCGAT+TGG | - | chr2.3:5821292-5821311 | MS.gene052120:CDS | 40.0% |
| !! | CTGTGATAATGGAACAGGAA+TGG | - | chr2.3:5821036-5821055 | MS.gene052120:CDS | 40.0% |
| !! | TTTTCATCAAGGCATCAGTG+AGG | + | chr2.3:5822175-5822194 | None:intergenic | 40.0% |
| AACAGCTTCCATTCCAACCA+TGG | + | chr2.3:5822417-5822436 | None:intergenic | 45.0% | |
| AAGAATAACCACGCTCGGTA+AGG | + | chr2.3:5822199-5822218 | None:intergenic | 45.0% | |
| AAGCAGCATGAAGATCAAGG+TGG | - | chr2.3:5822586-5822605 | MS.gene052120:intron | 45.0% | |
| ACCTTGATCTTCATGCTGCT+TGG | + | chr2.3:5822587-5822606 | None:intergenic | 45.0% | |
| ACGAAGGATAGCATGTGGAA+GGG | + | chr2.3:5822132-5822151 | None:intergenic | 45.0% | |
| AGGACCAGATTCATCGTACT+CGG | + | chr2.3:5822826-5822845 | None:intergenic | 45.0% | |
| ATAGTGATGATCTGGCCATC+AGG | + | chr2.3:5822350-5822369 | None:intergenic | 45.0% | |
| ATGAAAATCCTTACCGAGCG+TGG | - | chr2.3:5822188-5822207 | MS.gene052120:CDS | 45.0% | |
| ATGGTTGGAATGGAAGCTGT+TGG | - | chr2.3:5822416-5822435 | MS.gene052120:CDS | 45.0% | |
| CAGCTCCAATAGTGATGATC+TGG | + | chr2.3:5822358-5822377 | None:intergenic | 45.0% | |
| CATAGCCAAATCGCTTCTTC+AGG | + | chr2.3:5821103-5821122 | None:intergenic | 45.0% | |
| CATTGCTGACAGAATGAGCA+AGG | - | chr2.3:5822544-5822563 | MS.gene052120:intron | 45.0% | |
| CCGAGAGAAAATACAGTGTC+TGG | - | chr2.3:5822618-5822637 | MS.gene052120:intron | 45.0% | |
| CGCGGACAATGTGAGTATAT+CGG | + | chr2.3:5822694-5822713 | None:intergenic | 45.0% | |
| CTGGTCCTTCAATTGTACAC+AGG | - | chr2.3:5822838-5822857 | MS.gene052120:CDS | 45.0% | |
| GACCAGTGGTTGAATATCCT+CGG | + | chr2.3:5821017-5821036 | None:intergenic | 45.0% | |
| GATGGCCAGATCATCACTAT+TGG | - | chr2.3:5822350-5822369 | MS.gene052120:CDS | 45.0% | |
| GCTGTGTTTCCTAGCATTGT+TGG | - | chr2.3:5821428-5821447 | MS.gene052120:CDS | 45.0% | |
| GGAAAAGAACCTCAGGACAA+CGG | + | chr2.3:5822388-5822407 | None:intergenic | 45.0% | |
| GTTCCATTATCACAGACCAG+TGG | + | chr2.3:5821031-5821050 | None:intergenic | 45.0% | |
| GTTCTATCGCTCTATGCTAG+TGG | - | chr2.3:5821758-5821777 | MS.gene052120:CDS | 45.0% | |
| TATGCTAGTGGTCGAACAAC+CGG | - | chr2.3:5821770-5821789 | MS.gene052120:CDS | 45.0% | |
| TCAAGACGAAGGATAGCATG+TGG | + | chr2.3:5822137-5822156 | None:intergenic | 45.0% | |
| TCATTCTGTCAGCAATGCCA+GGG | + | chr2.3:5822541-5822560 | None:intergenic | 45.0% | |
| TGTGAGTAACTGGGATGACA+TGG | - | chr2.3:5821568-5821587 | MS.gene052120:CDS | 45.0% | |
| ! | ACACTGTATTTTCTCTCGGG+TGG | + | chr2.3:5822617-5822636 | None:intergenic | 45.0% |
| ! | ATTGTACTGGACTCTGGAGA+TGG | - | chr2.3:5822071-5822090 | MS.gene052120:intron | 45.0% |
| ! | CAACCACTGGTCTGTGATAA+TGG | - | chr2.3:5821025-5821044 | MS.gene052120:CDS | 45.0% |
| ! | CCTTGATCTTCATGCTGCTT+GGG | + | chr2.3:5822586-5822605 | None:intergenic | 45.0% |
| ! | CTTGATCTTCATGCTGCTTG+GGG | + | chr2.3:5822585-5822604 | None:intergenic | 45.0% |
| !! | CGATTAGCCTTTGGATTGAG+CGG | + | chr2.3:5821671-5821690 | None:intergenic | 45.0% |
| !! | CTGGTCTGTGATAATGGAAC+AGG | - | chr2.3:5821031-5821050 | MS.gene052120:CDS | 45.0% |
| AAGGCCGAGTACGATGAATC+TGG | - | chr2.3:5822819-5822838 | MS.gene052120:CDS | 50.0% | |
| ACTGGTGTGATGGTTGGCAT+GGG | - | chr2.3:5821464-5821483 | MS.gene052120:CDS | 50.0% | |
| ATGCTAGGAAACACAGCCCT+TGG | + | chr2.3:5821425-5821444 | None:intergenic | 50.0% | |
| CCCAAGCAGCATGAAGATCA+AGG | - | chr2.3:5822583-5822602 | MS.gene052120:intron | 50.0% | |
| CTCATTCTGTCAGCAATGCC+AGG | + | chr2.3:5822542-5822561 | None:intergenic | 50.0% | |
| GAAGGCTGGAAAAGAACCTC+AGG | + | chr2.3:5822395-5822414 | None:intergenic | 50.0% | |
| GACGAAGGATAGCATGTGGA+AGG | + | chr2.3:5822133-5822152 | None:intergenic | 50.0% | |
| GAGCAAGGAAATTACCGCGT+TGG | - | chr2.3:5822559-5822578 | MS.gene052120:intron | 50.0% | |
| GCCCTGCTAAATCAAGACGA+AGG | + | chr2.3:5822148-5822167 | None:intergenic | 50.0% | |
| GCTTCCATTCCAACCATGGA+AGG | + | chr2.3:5822413-5822432 | None:intergenic | 50.0% | |
| GGTGCTTCAGTGAGCAAAAC+CGG | + | chr2.3:5821650-5821669 | None:intergenic | 50.0% | |
| GGTGGTACTACTATGTTCCC+TGG | - | chr2.3:5822521-5822540 | MS.gene052120:intron | 50.0% | |
| GTGCTTCAGTGAGCAAAACC+GGG | + | chr2.3:5821649-5821668 | None:intergenic | 50.0% | |
| TACAATTTCACGCTCGGCTG+TGG | + | chr2.3:5822228-5822247 | None:intergenic | 50.0% | |
| TGCTGGAGATGATGCTCCAA+GGG | - | chr2.3:5821406-5821425 | MS.gene052120:CDS | 50.0% | |
| TTGCTGGAGATGATGCTCCA+AGG | - | chr2.3:5821405-5821424 | MS.gene052120:CDS | 50.0% | |
| ! | CTTTTCCAGCCTTCCATGGT+TGG | - | chr2.3:5822401-5822420 | MS.gene052120:CDS | 50.0% |
| ! | GCAGGTATTGTACTGGACTC+TGG | - | chr2.3:5822065-5822084 | MS.gene052120:intron | 50.0% |
| ! | GGTTCTTTTCCAGCCTTCCA+TGG | - | chr2.3:5822397-5822416 | MS.gene052120:CDS | 50.0% |
| !! | TGAAGCACCGCTCAATCCAA+AGG | - | chr2.3:5821661-5821680 | MS.gene052120:CDS | 50.0% |
| CACTGGTGTGATGGTTGGCA+TGG | - | chr2.3:5821463-5821482 | MS.gene052120:CDS | 55.0% | |
| CCAGCCTTCCATGGTTGGAA+TGG | - | chr2.3:5822406-5822425 | MS.gene052120:CDS | 55.0% | |
| CCATTCCAACCATGGAAGGC+TGG | + | chr2.3:5822409-5822428 | None:intergenic | 55.0% | |
| CGCCGAGGATATTCAACCAC+TGG | - | chr2.3:5821012-5821031 | MS.gene052120:CDS | 55.0% | |
| CGTGGTCGACCAACAATGCT+AGG | + | chr2.3:5821440-5821459 | None:intergenic | 55.0% | |
| GATGAAGCTCAGTCCAAGCG+TGG | - | chr2.3:5821509-5821528 | MS.gene052120:CDS | 55.0% | |
| TGAGCGTTTCCGTTGTCCTG+AGG | - | chr2.3:5822376-5822395 | MS.gene052120:CDS | 55.0% | |
| TGCCAACCATCACACCAGTG+TGG | + | chr2.3:5821463-5821482 | None:intergenic | 55.0% | |
| ACCACGCCACACTGGTGTGA+TGG | - | chr2.3:5821454-5821473 | MS.gene052120:CDS | 60.0% | |
| ACCATCACACCAGTGTGGCG+TGG | + | chr2.3:5821458-5821477 | None:intergenic | 60.0% | |
| AGCAAAACCGGGTGCTCCTC+TGG | + | chr2.3:5821638-5821657 | None:intergenic | 60.0% | |
| CAAAACCGGGTGCTCCTCTG+GGG | + | chr2.3:5821636-5821655 | None:intergenic | 60.0% | |
| CGCCACACTGGTGTGATGGT+TGG | - | chr2.3:5821458-5821477 | MS.gene052120:CDS | 60.0% | |
| GCAAAACCGGGTGCTCCTCT+GGG | + | chr2.3:5821637-5821656 | None:intergenic | 60.0% | |
| GTTGGTCGACCACGCCACAC+TGG | - | chr2.3:5821446-5821465 | MS.gene052120:CDS | 65.0% | |
| TGAACTCCGTGTGGCCCCAG+AGG | - | chr2.3:5821619-5821638 | MS.gene052120:CDS | 65.0% | |
| ! | CATGCTGCTTGGGGCCAACG+CGG | + | chr2.3:5822576-5822595 | None:intergenic | 65.0% |
| !! | GGTGCTCCTCTGGGGCCACA+CGG | + | chr2.3:5821628-5821647 | None:intergenic | 70.0% |
| !! | TGTGGCCCCAGAGGAGCACC+CGG | - | chr2.3:5821628-5821647 | MS.gene052120:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 5821004 | 5822872 | 5821004 | ID=MS.gene052120 |
| chr2.3 | mRNA | 5821004 | 5822872 | 5821004 | ID=MS.gene052120.t1;Parent=MS.gene052120 |
| chr2.3 | exon | 5822813 | 5822872 | 5822813 | ID=MS.gene052120.t1.exon1;Parent=MS.gene052120.t1 |
| chr2.3 | CDS | 5822813 | 5822872 | 5822813 | ID=cds.MS.gene052120.t1;Parent=MS.gene052120.t1 |
| chr2.3 | exon | 5822085 | 5822478 | 5822085 | ID=MS.gene052120.t1.exon2;Parent=MS.gene052120.t1 |
| chr2.3 | CDS | 5822085 | 5822478 | 5822085 | ID=cds.MS.gene052120.t1;Parent=MS.gene052120.t1 |
| chr2.3 | exon | 5821194 | 5821807 | 5821194 | ID=MS.gene052120.t1.exon3;Parent=MS.gene052120.t1 |
| chr2.3 | CDS | 5821194 | 5821807 | 5821194 | ID=cds.MS.gene052120.t1;Parent=MS.gene052120.t1 |
| chr2.3 | exon | 5821004 | 5821069 | 5821004 | ID=MS.gene052120.t1.exon4;Parent=MS.gene052120.t1 |
| chr2.3 | CDS | 5821004 | 5821069 | 5821004 | ID=cds.MS.gene052120.t1;Parent=MS.gene052120.t1 |
| Gene Sequence |
| Protein sequence |
