Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017992.t1 | XP_003625265.1 | 100 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 4.80E-217 | 763.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017992.t1 | P46258 | 98.9 | 377 | 4 | 0 | 1 | 377 | 1 | 377 | 1.8e-217 | 756.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017992.t1 | G7KVE1 | 100.0 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 3.4e-217 | 763.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017992.t1 | MTR_7g093260 | 100.000 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 0.0 | 785 |
| MS.gene017992.t1 | MTR_0015s0130 | 96.817 | 377 | 12 | 0 | 1 | 377 | 1 | 377 | 0.0 | 769 |
| MS.gene017992.t1 | MTR_2g096840 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 765 |
| MS.gene017992.t1 | MTR_6g004000 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene017992.t1 | MTR_3g095530 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene017992.t1 | MTR_7g026230 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene017992.t1 | MTR_2g008050 | 92.838 | 377 | 27 | 0 | 1 | 377 | 1 | 377 | 0.0 | 744 |
| MS.gene017992.t1 | MTR_7g027185 | 43.799 | 379 | 199 | 3 | 10 | 374 | 7 | 385 | 2.01e-120 | 354 |
| MS.gene017992.t1 | MTR_7g027185 | 45.070 | 355 | 182 | 2 | 10 | 351 | 7 | 361 | 9.32e-118 | 346 |
| MS.gene017992.t1 | MTR_7g027185 | 48.208 | 307 | 157 | 1 | 10 | 314 | 7 | 313 | 3.31e-110 | 325 |
| MS.gene017992.t1 | MTR_5g066400 | 37.136 | 447 | 207 | 12 | 1 | 376 | 1 | 444 | 1.61e-91 | 282 |
| MS.gene017992.t1 | MTR_8g464900 | 36.343 | 432 | 202 | 11 | 1 | 362 | 1 | 429 | 1.01e-84 | 264 |
| MS.gene017992.t1 | MTR_5g066400 | 34.529 | 446 | 192 | 12 | 1 | 376 | 1 | 416 | 9.86e-79 | 248 |
| MS.gene017992.t1 | MTR_8g089630 | 34.845 | 419 | 207 | 10 | 10 | 372 | 10 | 418 | 9.57e-72 | 230 |
| MS.gene017992.t1 | MTR_3g030550 | 37.766 | 376 | 208 | 9 | 11 | 377 | 5 | 363 | 2.48e-68 | 219 |
| MS.gene017992.t1 | MTR_5g066400 | 34.000 | 400 | 193 | 11 | 1 | 332 | 1 | 397 | 1.62e-67 | 219 |
| MS.gene017992.t1 | MTR_2g445210 | 27.869 | 427 | 242 | 7 | 4 | 366 | 2 | 426 | 3.20e-55 | 188 |
| MS.gene017992.t1 | MTR_8g007440 | 33.588 | 262 | 151 | 6 | 77 | 319 | 9 | 266 | 8.45e-45 | 155 |
| MS.gene017992.t1 | MTR_2g445210 | 26.330 | 376 | 211 | 7 | 4 | 315 | 2 | 375 | 2.80e-41 | 149 |
| MS.gene017992.t1 | MTR_3g010170 | 30.303 | 297 | 183 | 9 | 91 | 371 | 179 | 467 | 1.47e-39 | 146 |
| MS.gene017992.t1 | MTR_8g070520 | 31.058 | 293 | 175 | 10 | 91 | 366 | 187 | 469 | 2.41e-35 | 135 |
| MS.gene017992.t1 | MTR_8g070520 | 30.576 | 278 | 167 | 9 | 91 | 352 | 187 | 454 | 4.40e-32 | 126 |
| MS.gene017992.t1 | MTR_3g067945 | 29.762 | 252 | 158 | 7 | 9 | 245 | 21 | 268 | 3.59e-23 | 101 |
| MS.gene017992.t1 | MTR_3g067945 | 34.426 | 122 | 77 | 2 | 250 | 368 | 586 | 707 | 8.52e-16 | 79.3 |
| MS.gene017992.t1 | MTR_8g008540 | 70.270 | 37 | 11 | 0 | 340 | 376 | 41 | 77 | 4.15e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017992.t1 | AT5G09810 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MS.gene017992.t1 | AT3G12110 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 760 |
| MS.gene017992.t1 | AT3G46520 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT3G53750 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT3G53750 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT3G46520 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene017992.t1 | AT5G59370 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 758 |
| MS.gene017992.t1 | AT5G59370 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 758 |
| MS.gene017992.t1 | AT3G18780 | 90.981 | 377 | 34 | 0 | 1 | 377 | 1 | 377 | 0.0 | 737 |
| MS.gene017992.t1 | AT3G18780 | 90.981 | 377 | 34 | 0 | 1 | 377 | 1 | 377 | 0.0 | 737 |
| MS.gene017992.t1 | AT1G49240 | 90.716 | 377 | 35 | 0 | 1 | 377 | 1 | 377 | 0.0 | 735 |
| MS.gene017992.t1 | AT3G18780 | 90.808 | 359 | 33 | 0 | 1 | 359 | 1 | 359 | 0.0 | 696 |
| MS.gene017992.t1 | AT2G42100 | 85.326 | 368 | 54 | 0 | 10 | 377 | 11 | 378 | 0.0 | 677 |
| MS.gene017992.t1 | AT2G42090 | 71.351 | 370 | 101 | 1 | 7 | 376 | 1 | 365 | 0.0 | 568 |
| MS.gene017992.t1 | AT2G42170 | 78.313 | 332 | 69 | 1 | 46 | 377 | 1 | 329 | 0.0 | 560 |
| MS.gene017992.t1 | AT2G42170 | 78.313 | 332 | 69 | 1 | 46 | 377 | 1 | 329 | 0.0 | 560 |
| MS.gene017992.t1 | AT2G42170 | 78.205 | 312 | 65 | 1 | 46 | 357 | 1 | 309 | 0.0 | 522 |
| MS.gene017992.t1 | AT2G42170 | 78.205 | 312 | 65 | 1 | 46 | 357 | 1 | 309 | 0.0 | 522 |
| MS.gene017992.t1 | AT2G42170 | 78.761 | 226 | 45 | 1 | 152 | 377 | 9 | 231 | 5.79e-132 | 377 |
| MS.gene017992.t1 | AT3G27000 | 44.591 | 379 | 196 | 4 | 10 | 374 | 7 | 385 | 2.61e-122 | 358 |
| MS.gene017992.t1 | AT1G18450 | 36.569 | 443 | 211 | 9 | 1 | 376 | 1 | 440 | 7.98e-94 | 288 |
| MS.gene017992.t1 | AT1G13180 | 34.615 | 416 | 212 | 11 | 10 | 372 | 9 | 417 | 7.01e-72 | 231 |
| MS.gene017992.t1 | AT3G60830 | 37.731 | 379 | 212 | 9 | 7 | 377 | 1 | 363 | 1.19e-67 | 218 |
| MS.gene017992.t1 | AT3G33520 | 30.166 | 421 | 236 | 7 | 10 | 377 | 5 | 420 | 6.97e-59 | 197 |
| MS.gene017992.t1 | AT5G56180 | 30.986 | 284 | 171 | 9 | 91 | 358 | 50 | 324 | 2.21e-34 | 130 |
| MS.gene017992.t1 | AT5G56180 | 30.986 | 284 | 171 | 9 | 91 | 358 | 182 | 456 | 1.20e-33 | 130 |
| MS.gene017992.t1 | AT3G12380 | 31.395 | 258 | 152 | 9 | 9 | 245 | 21 | 274 | 8.24e-26 | 109 |
| MS.gene017992.t1 | AT3G12380 | 33.103 | 145 | 93 | 2 | 228 | 368 | 572 | 716 | 3.20e-16 | 80.9 |
| MS.gene017992.t1 | AT3G12380 | 31.250 | 256 | 149 | 9 | 9 | 245 | 21 | 268 | 3.66e-25 | 107 |
| MS.gene017992.t1 | AT3G12380 | 33.103 | 145 | 93 | 2 | 228 | 368 | 566 | 710 | 3.22e-16 | 80.9 |
| MS.gene017992.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.58e-23 | 95.1 |
| MS.gene017992.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.58e-23 | 95.1 |
| MS.gene017992.t1 | AT5G56180 | 32.292 | 192 | 110 | 7 | 91 | 271 | 182 | 364 | 8.79e-19 | 87.4 |
| MS.gene017992.t1 | AT1G73910 | 29.268 | 123 | 71 | 2 | 1 | 107 | 1 | 123 | 3.24e-12 | 64.7 |
Find 104 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGGTATTGTTCTGGATTC+TGG | 0.149133 | 7.4:+15594622 | MS.gene017992:intron |
| AGCATGGGATTGTTAGTAAT+TGG | 0.164954 | 7.4:+15594045 | MS.gene017992:CDS |
| CTGGATTGGAGGTTCCATTT+TGG | 0.195803 | 7.4:+15595194 | MS.gene017992:CDS |
| TGTTAGTAATTGGGATGATA+TGG | 0.286824 | 7.4:+15594055 | MS.gene017992:CDS |
| AGAAGAACAGGGTGTTCTTC+AGG | 0.300324 | 7.4:-15594122 | None:intergenic |
| GATGGCTGGAACAAAACTTC+TGG | 0.305788 | 7.4:-15594949 | None:intergenic |
| GAGGGATATGAAGGAGAAAT+TGG | 0.306130 | 7.4:+15594804 | MS.gene017992:CDS |
| CATTTCCTGTGTACTATTGA+TGG | 0.320989 | 7.4:-15595449 | None:intergenic |
| TCCCCATGCAATCCTTCGTT+TGG | 0.323983 | 7.4:+15594690 | MS.gene017992:CDS |
| ATCCTTCGTTTGGATCTTGC+AGG | 0.332165 | 7.4:+15594700 | MS.gene017992:CDS |
| CTAACAATCCCATGCTCAAT+TGG | 0.332451 | 7.4:-15594038 | None:intergenic |
| TTGTTCCAGCCATCCATGAT+AGG | 0.341621 | 7.4:+15594958 | MS.gene017992:CDS |
| GGTGCTTCAGTGAGAAGAAC+AGG | 0.346523 | 7.4:-15594134 | None:intergenic |
| AGTGCATATCCCTCGTAAAT+AGG | 0.354141 | 7.4:-15594667 | None:intergenic |
| GGTGGTTCTACAATGTTTCC+TGG | 0.367242 | 7.4:+15595078 | MS.gene017992:CDS |
| AAGGCAGAATATGATGAATC+TGG | 0.369605 | 7.4:+15595425 | MS.gene017992:CDS |
| TAGGTATTGTTCTGGATTCT+GGG | 0.386484 | 7.4:+15594623 | MS.gene017992:intron |
| AGTGCTGAGGGATGCCAAAA+TGG | 0.398050 | 7.4:-15595208 | None:intergenic |
| CGATTAGCCTTAGGATTAAG+AGG | 0.422207 | 7.4:-15594155 | None:intergenic |
| TGAAGTGTGATGTCGACATT+AGG | 0.428549 | 7.4:+15595025 | MS.gene017992:CDS |
| ATCCCTCAGCACTTTCCAAC+AGG | 0.435296 | 7.4:+15595218 | MS.gene017992:CDS |
| GGTTCCGTTGTCGCAAACTA+AGG | 0.435984 | 7.4:-15593640 | None:intergenic |
| TATGCGAGTGGTCGTACAAC+TGG | 0.440352 | 7.4:+15594257 | MS.gene017992:CDS |
| GCAGTATTCCCTAGCATAGT+AGG | 0.445310 | 7.4:+15593915 | MS.gene017992:CDS |
| GCATGGGATTGTTAGTAATT+GGG | 0.447989 | 7.4:+15594046 | MS.gene017992:CDS |
| ATTGTGATCACCTGCCCATC+AGG | 0.453493 | 7.4:-15594904 | None:intergenic |
| TGTTTCCTGGTATTGCTGAT+AGG | 0.455278 | 7.4:+15595091 | MS.gene017992:CDS |
| GACATTAGGAAAGATCTCTA+TGG | 0.455677 | 7.4:+15595039 | MS.gene017992:CDS |
| TTAGTTTGCGACAACGGAAC+CGG | 0.471674 | 7.4:+15593642 | MS.gene017992:CDS |
| CAGAGAGGAAGTACAGTGTC+TGG | 0.471867 | 7.4:+15595175 | MS.gene017992:CDS |
| ATGGCTGGAACAAAACTTCT+GGG | 0.473579 | 7.4:-15594948 | None:intergenic |
| TATTGCTCTTGATTATGAGC+AGG | 0.475672 | 7.4:+15594831 | MS.gene017992:CDS |
| AGGAGATTCCATCCCTATCA+TGG | 0.480685 | 7.4:-15594971 | None:intergenic |
| GGGATGATATGGAGAAGATC+TGG | 0.484705 | 7.4:+15594066 | MS.gene017992:CDS |
| AGGAAGTACAGTGTCTGGAT+TGG | 0.485496 | 7.4:+15595180 | MS.gene017992:CDS |
| ATTGTTCTGGATTCTGGGGA+TGG | 0.491654 | 7.4:+15594628 | MS.gene017992:CDS |
| CAGACACTGTACTTCCTCTC+TGG | 0.493244 | 7.4:-15595174 | None:intergenic |
| CACAGGGGTAATGGTTGGAA+TGG | 0.495803 | 7.4:+15593950 | MS.gene017992:CDS |
| AATTTCTCGCTCTGCTGAAG+TGG | 0.496387 | 7.4:-15594779 | None:intergenic |
| TAACAATCCCATGCTCAATT+GGG | 0.497649 | 7.4:-15594037 | None:intergenic |
| CTGCCCATCAGGTAGCTCAT+AGG | 0.497821 | 7.4:-15594893 | None:intergenic |
| CGTGGTCGTCCTACTATGCT+AGG | 0.500170 | 7.4:-15593924 | None:intergenic |
| AGAAATTGTGAGGGATATGA+AGG | 0.501249 | 7.4:+15594795 | MS.gene017992:CDS |
| AAGTACAGTGTCTGGATTGG+AGG | 0.501277 | 7.4:+15595183 | MS.gene017992:CDS |
| GATCCAAACGAAGGATTGCA+TGG | 0.503842 | 7.4:-15594693 | None:intergenic |
| ACCACGTCACACAGGGGTAA+TGG | 0.512712 | 7.4:+15593941 | MS.gene017992:CDS |
| TGTTCCAGCCATCCATGATA+GGG | 0.514648 | 7.4:+15594959 | MS.gene017992:CDS |
| ACAGGGGTAATGGTTGGAAT+GGG | 0.516861 | 7.4:+15593951 | MS.gene017992:CDS |
| TTGAAATACCCAATTGAGCA+TGG | 0.529351 | 7.4:+15594029 | MS.gene017992:CDS |
| CTATGAGCTACCTGATGGGC+AGG | 0.530662 | 7.4:+15594894 | MS.gene017992:CDS |
| GATGGGCAGGTGATCACAAT+CGG | 0.534348 | 7.4:+15594907 | MS.gene017992:CDS |
| CCATCCCTATCATGGATGGC+TGG | 0.535688 | 7.4:-15594963 | None:intergenic |
| AATGTTGTCTCGTGGATACC+AGG | 0.544087 | 7.4:-15594991 | None:intergenic |
| TGCAGGAGATGATGCACCAA+GGG | 0.547290 | 7.4:+15593893 | MS.gene017992:CDS |
| CTCATCCTATCAGCAATACC+AGG | 0.549889 | 7.4:-15595096 | None:intergenic |
| GAGCAAGGAGATTACAGCAT+TGG | 0.550032 | 7.4:+15595116 | MS.gene017992:CDS |
| CAGCCCTTAGTTTGCGACAA+CGG | 0.557180 | 7.4:+15593636 | MS.gene017992:CDS |
| TCTTGATTATGAGCAGGAGT+TGG | 0.557428 | 7.4:+15594837 | MS.gene017992:CDS |
| TGAATAGCAACATACATAGC+AGG | 0.559953 | 7.4:-15594218 | None:intergenic |
| GTGCTTTCGCTTTATGCGAG+TGG | 0.560364 | 7.4:+15594245 | MS.gene017992:CDS |
| GGAACAAAACTTCTGGGCAT+CGG | 0.561285 | 7.4:-15594942 | None:intergenic |
| AGGCAGAATATGATGAATCT+GGG | 0.564408 | 7.4:+15595426 | MS.gene017992:CDS |
| TGAAGCACCTCTTAATCCTA+AGG | 0.566737 | 7.4:+15594148 | MS.gene017992:CDS |
| ATCCAAACGAAGGATTGCAT+GGG | 0.568962 | 7.4:-15594692 | None:intergenic |
| CAACGGAACCGGCATGGTCA+AGG | 0.570249 | 7.4:+15593653 | MS.gene017992:CDS |
| ACCTAGTAGCATGAAAATCA+AGG | 0.573048 | 7.4:+15595140 | MS.gene017992:CDS |
| GGTGAAAGTGTAACCACGCT+CGG | 0.573261 | 7.4:-15594758 | None:intergenic |
| TGAAATACCCAATTGAGCAT+GGG | 0.576183 | 7.4:+15594030 | MS.gene017992:CDS |
| ATGCTAGGGAATACTGCCCT+TGG | 0.578562 | 7.4:-15593909 | None:intergenic |
| ATGATAGGGATGGAATCTCC+TGG | 0.579865 | 7.4:+15594973 | MS.gene017992:CDS |
| GTTCCGTTGTCGCAAACTAA+GGG | 0.584635 | 7.4:-15593639 | None:intergenic |
| TATGGTAACATTGTGTTGAG+TGG | 0.586496 | 7.4:+15595057 | MS.gene017992:CDS |
| CGTCACACAGGGGTAATGGT+TGG | 0.589613 | 7.4:+15593945 | MS.gene017992:CDS |
| GGTAACATTGTGTTGAGTGG+TGG | 0.592071 | 7.4:+15595060 | MS.gene017992:CDS |
| TTACCTGTTGGAAAGTGCTG+AGG | 0.592935 | 7.4:-15595221 | None:intergenic |
| GATTGATACCTTGACCATGC+CGG | 0.593626 | 7.4:-15593661 | None:intergenic |
| AAGACCTATGAGCTACCTGA+TGG | 0.595691 | 7.4:+15594889 | MS.gene017992:CDS |
| GATTCCATCCCTATCATGGA+TGG | 0.610301 | 7.4:-15594967 | None:intergenic |
| ACCATTACCCCTGTGTGACG+TGG | 0.613863 | 7.4:-15593942 | None:intergenic |
| TAGGACGACCACGTCACACA+GGG | 0.616704 | 7.4:+15593934 | MS.gene017992:CDS |
| GTGGTCGTCCTACTATGCTA+GGG | 0.619760 | 7.4:-15593923 | None:intergenic |
| CCAGCCATCCATGATAGGGA+TGG | 0.621684 | 7.4:+15594963 | MS.gene017992:CDS |
| TCGAGTTAAATGTTGTCTCG+TGG | 0.622628 | 7.4:-15594999 | None:intergenic |
| CAGCAGAGCGAGAAATTGTG+AGG | 0.625485 | 7.4:+15594785 | MS.gene017992:CDS |
| ACACTGTACTTCCTCTCTGG+TGG | 0.626941 | 7.4:-15595171 | None:intergenic |
| TTGCGACAACGGAACCGGCA+TGG | 0.628011 | 7.4:+15593647 | MS.gene017992:CDS |
| AGGTATTGTTCTGGATTCTG+GGG | 0.628541 | 7.4:+15594624 | MS.gene017992:intron |
| GTAGGACGACCACGTCACAC+AGG | 0.630344 | 7.4:+15593933 | MS.gene017992:CDS |
| AGACCTATGAGCTACCTGAT+GGG | 0.631032 | 7.4:+15594890 | MS.gene017992:CDS |
| TGGGGATGGTGTTAGTCACA+CGG | 0.632223 | 7.4:+15594642 | MS.gene017992:CDS |
| TATTGCTGATAGGATGAGCA+AGG | 0.645350 | 7.4:+15595101 | MS.gene017992:CDS |
| CTGGGCCATCAATAGTACAC+AGG | 0.648814 | 7.4:+15595444 | MS.gene017992:CDS |
| TACCTGTTGGAAAGTGCTGA+GGG | 0.649811 | 7.4:-15595220 | None:intergenic |
| GATGAAGCACAATCAAAGAG+AGG | 0.658995 | 7.4:+15593996 | MS.gene017992:CDS |
| CACACGGTTCCTATTTACGA+GGG | 0.661422 | 7.4:+15594658 | MS.gene017992:CDS |
| TTGCAGGAGATGATGCACCA+AGG | 0.664250 | 7.4:+15593892 | MS.gene017992:CDS |
| AGCAGAGCGAGAAATTGTGA+GGG | 0.666151 | 7.4:+15594786 | MS.gene017992:CDS |
| TCACACGGTTCCTATTTACG+AGG | 0.670052 | 7.4:+15594657 | MS.gene017992:CDS |
| GTGCTTCAGTGAGAAGAACA+GGG | 0.670285 | 7.4:-15594133 | None:intergenic |
| TCCAAACGAAGGATTGCATG+GGG | 0.676900 | 7.4:-15594691 | None:intergenic |
| TGTGCAGATGTGGATTGCAA+AGG | 0.694315 | 7.4:+15595406 | MS.gene017992:intron |
| GACCTGCAAGATCCAAACGA+AGG | 0.699076 | 7.4:-15594702 | None:intergenic |
| AGGTTGTAGCACCACCAGAG+AGG | 0.701742 | 7.4:+15595160 | MS.gene017992:CDS |
| AGGACGACCACGTCACACAG+GGG | 0.764581 | 7.4:+15593935 | MS.gene017992:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCCATTTTTTAAATTATTAC+CGG | - | chr7.4:15594415-15594434 | None:intergenic | 15.0% |
| !!! | TTATGTTTTGAAGTTATTAT+CGG | + | chr7.4:15593787-15593806 | MS.gene017992:intron | 15.0% |
| !! | ACCGGTAATAATTTAAAAAA+TGG | + | chr7.4:15594411-15594430 | MS.gene017992:intron | 20.0% |
| !!! | CATAGATTTAACTTTTTTTG+TGG | + | chr7.4:15593853-15593872 | MS.gene017992:intron | 20.0% |
| ! | AAGTACAGGTTATAAAAAGT+AGG | + | chr7.4:15595304-15595323 | MS.gene017992:intron | 25.0% |
| ! | GAATGATGAATATGATTTGA+AGG | + | chr7.4:15594530-15594549 | MS.gene017992:intron | 25.0% |
| ! | TGATTCATACAAAACTCATA+CGG | - | chr7.4:15594358-15594377 | None:intergenic | 25.0% |
| ! | TTCATTTCAACTATAAGTAC+AGG | + | chr7.4:15595290-15595309 | MS.gene017992:intron | 25.0% |
| !! | ATAACAAATGTGTTTTACTG+AGG | + | chr7.4:15595332-15595351 | MS.gene017992:intron | 25.0% |
| !!! | ACTTTTTTTGTGGTTGTTTT+AGG | + | chr7.4:15593863-15593882 | MS.gene017992:intron | 25.0% |
| AATCCATCAAATTTACCTGT+TGG | - | chr7.4:15595236-15595255 | None:intergenic | 30.0% | |
| TACAGGTTATAAAAAGTAGG+AGG | + | chr7.4:15595307-15595326 | MS.gene017992:intron | 30.0% | |
| TGTTAGTAATTGGGATGATA+TGG | + | chr7.4:15594055-15594074 | MS.gene017992:CDS | 30.0% | |
| TTTCCAACAGGTAAATTTGA+TGG | + | chr7.4:15595230-15595249 | MS.gene017992:intron | 30.0% | |
| ! | TGATTTTCTGTGTTTTGTGT+AGG | + | chr7.4:15594604-15594623 | MS.gene017992:intron | 30.0% |
| !!! | TTTTGAAGTTATTATCGGTG+TGG | + | chr7.4:15593792-15593811 | MS.gene017992:intron | 30.0% |
| AAGGCAGAATATGATGAATC+TGG | + | chr7.4:15595425-15595444 | MS.gene017992:CDS | 35.0% | |
| ACCTAGTAGCATGAAAATCA+AGG | + | chr7.4:15595140-15595159 | MS.gene017992:CDS | 35.0% | |
| AGAAATTGTGAGGGATATGA+AGG | + | chr7.4:15594795-15594814 | MS.gene017992:CDS | 35.0% | |
| AGCATGGGATTGTTAGTAAT+TGG | + | chr7.4:15594045-15594064 | MS.gene017992:CDS | 35.0% | |
| AGGCAGAATATGATGAATCT+GGG | + | chr7.4:15595426-15595445 | MS.gene017992:CDS | 35.0% | |
| CAAGATTGAAGGATAAACAC+CGG | + | chr7.4:15594393-15594412 | MS.gene017992:intron | 35.0% | |
| CATTTCCTGTGTACTATTGA+TGG | - | chr7.4:15595452-15595471 | None:intergenic | 35.0% | |
| GAATGTAATCACATATGTCG+TGG | + | chr7.4:15594450-15594469 | MS.gene017992:intron | 35.0% | |
| GACATTAGGAAAGATCTCTA+TGG | + | chr7.4:15595039-15595058 | MS.gene017992:CDS | 35.0% | |
| GCATGGGATTGTTAGTAATT+GGG | + | chr7.4:15594046-15594065 | MS.gene017992:CDS | 35.0% | |
| GGACAAAAAGATGCATATGT+TGG | + | chr7.4:15593972-15593991 | MS.gene017992:CDS | 35.0% | |
| TAACAATCCCATGCTCAATT+GGG | - | chr7.4:15594040-15594059 | None:intergenic | 35.0% | |
| TATGGTAACATTGTGTTGAG+TGG | + | chr7.4:15595057-15595076 | MS.gene017992:CDS | 35.0% | |
| TATTGCTCTTGATTATGAGC+AGG | + | chr7.4:15594831-15594850 | MS.gene017992:CDS | 35.0% | |
| TGAAATACCCAATTGAGCAT+GGG | + | chr7.4:15594030-15594049 | MS.gene017992:CDS | 35.0% | |
| TGAATAGCAACATACATAGC+AGG | - | chr7.4:15594221-15594240 | None:intergenic | 35.0% | |
| TTGAAATACCCAATTGAGCA+TGG | + | chr7.4:15594029-15594048 | MS.gene017992:CDS | 35.0% | |
| ! | ACCTTGATTTTCATGCTACT+AGG | - | chr7.4:15595144-15595163 | None:intergenic | 35.0% |
| ! | TAGGTATTGTTCTGGATTCT+GGG | + | chr7.4:15594623-15594642 | MS.gene017992:intron | 35.0% |
| !! | GTTTTGTGTAGGTATTGTTC+TGG | + | chr7.4:15594615-15594634 | MS.gene017992:intron | 35.0% |
| !!! | ATTTTCTCACGATTAGCCTT+AGG | - | chr7.4:15594167-15594186 | None:intergenic | 35.0% |
| !!! | TTTTTGTGGTTGTTTTAGGC+TGG | + | chr7.4:15593867-15593886 | MS.gene017992:intron | 35.0% |
| AGTGCATATCCCTCGTAAAT+AGG | - | chr7.4:15594670-15594689 | None:intergenic | 40.0% | |
| ATCCAAACGAAGGATTGCAT+GGG | - | chr7.4:15594695-15594714 | None:intergenic | 40.0% | |
| ATGGCTGGAACAAAACTTCT+GGG | - | chr7.4:15594951-15594970 | None:intergenic | 40.0% | |
| CGGTGTTTATCCTTCAATCT+TGG | - | chr7.4:15594395-15594414 | None:intergenic | 40.0% | |
| CTAACAATCCCATGCTCAAT+TGG | - | chr7.4:15594041-15594060 | None:intergenic | 40.0% | |
| GAGGGATATGAAGGAGAAAT+TGG | + | chr7.4:15594804-15594823 | MS.gene017992:CDS | 40.0% | |
| GATGAAGCACAATCAAAGAG+AGG | + | chr7.4:15593996-15594015 | MS.gene017992:CDS | 40.0% | |
| GCCATGTGATGATACAAACT+AGG | + | chr7.4:15594557-15594576 | MS.gene017992:intron | 40.0% | |
| TATGATCAAAAGCTCGTGTC+CGG | + | chr7.4:15593720-15593739 | MS.gene017992:intron | 40.0% | |
| TATTGCTGATAGGATGAGCA+AGG | + | chr7.4:15595101-15595120 | MS.gene017992:CDS | 40.0% | |
| TCGAGTTAAATGTTGTCTCG+TGG | - | chr7.4:15595002-15595021 | None:intergenic | 40.0% | |
| TCTTGATTATGAGCAGGAGT+TGG | + | chr7.4:15594837-15594856 | MS.gene017992:CDS | 40.0% | |
| TGAAGTGTGATGTCGACATT+AGG | + | chr7.4:15595025-15595044 | MS.gene017992:CDS | 40.0% | |
| TGTAATCACATATGTCGTGG+TGG | + | chr7.4:15594453-15594472 | MS.gene017992:intron | 40.0% | |
| TGTTTCCTGGTATTGCTGAT+AGG | + | chr7.4:15595091-15595110 | MS.gene017992:CDS | 40.0% | |
| ! | AGGTATTGTTCTGGATTCTG+GGG | + | chr7.4:15594624-15594643 | MS.gene017992:intron | 40.0% |
| ! | AGTATGTAGCATCGAAACGT+TGG | - | chr7.4:15594500-15594519 | None:intergenic | 40.0% |
| ! | GCCTAGTTTGTATCATCACA+TGG | - | chr7.4:15594561-15594580 | None:intergenic | 40.0% |
| ! | GTAGGTATTGTTCTGGATTC+TGG | + | chr7.4:15594622-15594641 | MS.gene017992:intron | 40.0% |
| ! | TACTTTTGCTTGTGCAGATG+TGG | + | chr7.4:15595396-15595415 | MS.gene017992:intron | 40.0% |
| ! | TGAAGCACCTCTTAATCCTA+AGG | + | chr7.4:15594148-15594167 | MS.gene017992:CDS | 40.0% |
| !! | ATGAAGATTTTAACCGAGCG+TGG | + | chr7.4:15594745-15594764 | MS.gene017992:CDS | 40.0% |
| !! | CGATTAGCCTTAGGATTAAG+AGG | - | chr7.4:15594158-15594177 | None:intergenic | 40.0% |
| !!! | TTGTTTTAGGCTGGTTTTGC+AGG | + | chr7.4:15593876-15593895 | MS.gene017992:intron | 40.0% |
| AAGACCTATGAGCTACCTGA+TGG | + | chr7.4:15594889-15594908 | MS.gene017992:CDS | 45.0% | |
| AATGTTGTCTCGTGGATACC+AGG | - | chr7.4:15594994-15595013 | None:intergenic | 45.0% | |
| AATTTCTCGCTCTGCTGAAG+TGG | - | chr7.4:15594782-15594801 | None:intergenic | 45.0% | |
| ACACACGACACCAAGATTGA+AGG | + | chr7.4:15594382-15594401 | MS.gene017992:intron | 45.0% | |
| AGACCTATGAGCTACCTGAT+GGG | + | chr7.4:15594890-15594909 | MS.gene017992:CDS | 45.0% | |
| AGCAGAGCGAGAAATTGTGA+GGG | + | chr7.4:15594786-15594805 | MS.gene017992:CDS | 45.0% | |
| AGGAGATTCCATCCCTATCA+TGG | - | chr7.4:15594974-15594993 | None:intergenic | 45.0% | |
| ATCCTTCGTTTGGATCTTGC+AGG | + | chr7.4:15594700-15594719 | MS.gene017992:CDS | 45.0% | |
| ATGATAGGGATGGAATCTCC+TGG | + | chr7.4:15594973-15594992 | MS.gene017992:CDS | 45.0% | |
| CACACGGTTCCTATTTACGA+GGG | + | chr7.4:15594658-15594677 | MS.gene017992:CDS | 45.0% | |
| CTCATCCTATCAGCAATACC+AGG | - | chr7.4:15595099-15595118 | None:intergenic | 45.0% | |
| GAGCAAGGAGATTACAGCAT+TGG | + | chr7.4:15595116-15595135 | MS.gene017992:CDS | 45.0% | |
| GATCCAAACGAAGGATTGCA+TGG | - | chr7.4:15594696-15594715 | None:intergenic | 45.0% | |
| GATGGCTGGAACAAAACTTC+TGG | - | chr7.4:15594952-15594971 | None:intergenic | 45.0% | |
| GATTCCATCCCTATCATGGA+TGG | - | chr7.4:15594970-15594989 | None:intergenic | 45.0% | |
| GATTGATACCTTGACCATGC+CGG | - | chr7.4:15593664-15593683 | None:intergenic | 45.0% | |
| GCAGTATTCCCTAGCATAGT+AGG | + | chr7.4:15593915-15593934 | MS.gene017992:CDS | 45.0% | |
| GGAACAAAACTTCTGGGCAT+CGG | - | chr7.4:15594945-15594964 | None:intergenic | 45.0% | |
| GGGATGATATGGAGAAGATC+TGG | + | chr7.4:15594066-15594085 | MS.gene017992:CDS | 45.0% | |
| GGTAACATTGTGTTGAGTGG+TGG | + | chr7.4:15595060-15595079 | MS.gene017992:CDS | 45.0% | |
| GTGCTTCAGTGAGAAGAACA+GGG | - | chr7.4:15594136-15594155 | None:intergenic | 45.0% | |
| GTTCCGTTGTCGCAAACTAA+GGG | - | chr7.4:15593642-15593661 | None:intergenic | 45.0% | |
| TCACACGGTTCCTATTTACG+AGG | + | chr7.4:15594657-15594676 | MS.gene017992:CDS | 45.0% | |
| TCCAAACGAAGGATTGCATG+GGG | - | chr7.4:15594694-15594713 | None:intergenic | 45.0% | |
| TGTTCCAGCCATCCATGATA+GGG | + | chr7.4:15594959-15594978 | MS.gene017992:CDS | 45.0% | |
| TTGTTCCAGCCATCCATGAT+AGG | + | chr7.4:15594958-15594977 | MS.gene017992:CDS | 45.0% | |
| ! | AAGTACAGTGTCTGGATTGG+AGG | + | chr7.4:15595183-15595202 | MS.gene017992:CDS | 45.0% |
| ! | ACAGGGGTAATGGTTGGAAT+GGG | + | chr7.4:15593951-15593970 | MS.gene017992:CDS | 45.0% |
| ! | AGAAGAACAGGGTGTTCTTC+AGG | - | chr7.4:15594125-15594144 | None:intergenic | 45.0% |
| ! | AGGAAGTACAGTGTCTGGAT+TGG | + | chr7.4:15595180-15595199 | MS.gene017992:CDS | 45.0% |
| ! | ATTGTTCTGGATTCTGGGGA+TGG | + | chr7.4:15594628-15594647 | MS.gene017992:CDS | 45.0% |
| ! | GGTGGTTCTACAATGTTTCC+TGG | + | chr7.4:15595078-15595097 | MS.gene017992:CDS | 45.0% |
| ! | TGTGCAGATGTGGATTGCAA+AGG | + | chr7.4:15595406-15595425 | MS.gene017992:intron | 45.0% |
| !! | CTGGATTGGAGGTTCCATTT+TGG | + | chr7.4:15595194-15595213 | MS.gene017992:CDS | 45.0% |
| !! | TACCTGTTGGAAAGTGCTGA+GGG | - | chr7.4:15595223-15595242 | None:intergenic | 45.0% |
| !! | TTACCTGTTGGAAAGTGCTG+AGG | - | chr7.4:15595224-15595243 | None:intergenic | 45.0% |
| !! | TTAGTTTGCGACAACGGAAC+CGG | + | chr7.4:15593642-15593661 | MS.gene017992:CDS | 45.0% |
| ACACTGTACTTCCTCTCTGG+TGG | - | chr7.4:15595174-15595193 | None:intergenic | 50.0% | |
| ATCCCTCAGCACTTTCCAAC+AGG | + | chr7.4:15595218-15595237 | MS.gene017992:CDS | 50.0% | |
| ATGCTAGGGAATACTGCCCT+TGG | - | chr7.4:15593912-15593931 | None:intergenic | 50.0% | |
| ATTGTGATCACCTGCCCATC+AGG | - | chr7.4:15594907-15594926 | None:intergenic | 50.0% | |
| CAGACACTGTACTTCCTCTC+TGG | - | chr7.4:15595177-15595196 | None:intergenic | 50.0% | |
| CAGCAGAGCGAGAAATTGTG+AGG | + | chr7.4:15594785-15594804 | MS.gene017992:CDS | 50.0% | |
| CTGGGCCATCAATAGTACAC+AGG | + | chr7.4:15595444-15595463 | MS.gene017992:CDS | 50.0% | |
| GACCTGCAAGATCCAAACGA+AGG | - | chr7.4:15594705-15594724 | None:intergenic | 50.0% | |
| GATGGGCAGGTGATCACAAT+CGG | + | chr7.4:15594907-15594926 | MS.gene017992:CDS | 50.0% | |
| GGATGTGTCAGTGTCGTATC+AGG | + | chr7.4:15593813-15593832 | MS.gene017992:intron | 50.0% | |
| GGTGCTTCAGTGAGAAGAAC+AGG | - | chr7.4:15594137-15594156 | None:intergenic | 50.0% | |
| GGTTCCGTTGTCGCAAACTA+AGG | - | chr7.4:15593643-15593662 | None:intergenic | 50.0% | |
| GTGCTTTCGCTTTATGCGAG+TGG | + | chr7.4:15594245-15594264 | MS.gene017992:CDS | 50.0% | |
| GTGGTCGTCCTACTATGCTA+GGG | - | chr7.4:15593926-15593945 | None:intergenic | 50.0% | |
| TATGCGAGTGGTCGTACAAC+TGG | + | chr7.4:15594257-15594276 | MS.gene017992:CDS | 50.0% | |
| TCCCCATGCAATCCTTCGTT+TGG | + | chr7.4:15594690-15594709 | MS.gene017992:CDS | 50.0% | |
| TGCAGGAGATGATGCACCAA+GGG | + | chr7.4:15593893-15593912 | MS.gene017992:CDS | 50.0% | |
| TTGCAGGAGATGATGCACCA+AGG | + | chr7.4:15593892-15593911 | MS.gene017992:CDS | 50.0% | |
| ! | AGTGCTGAGGGATGCCAAAA+TGG | - | chr7.4:15595211-15595230 | None:intergenic | 50.0% |
| ! | CACAGGGGTAATGGTTGGAA+TGG | + | chr7.4:15593950-15593969 | MS.gene017992:CDS | 50.0% |
| ! | CAGAGAGGAAGTACAGTGTC+TGG | + | chr7.4:15595175-15595194 | MS.gene017992:CDS | 50.0% |
| ! | GGTGAAAGTGTAACCACGCT+CGG | - | chr7.4:15594761-15594780 | None:intergenic | 50.0% |
| ! | TGGGGATGGTGTTAGTCACA+CGG | + | chr7.4:15594642-15594661 | MS.gene017992:CDS | 50.0% |
| !! | CAATCTTGGTGTCGTGTGTC+TGG | - | chr7.4:15594381-15594400 | None:intergenic | 50.0% |
| !! | CAGCCCTTAGTTTGCGACAA+CGG | + | chr7.4:15593636-15593655 | MS.gene017992:CDS | 50.0% |
| ACCACGTCACACAGGGGTAA+TGG | + | chr7.4:15593941-15593960 | MS.gene017992:CDS | 55.0% | |
| ACCATTACCCCTGTGTGACG+TGG | - | chr7.4:15593945-15593964 | None:intergenic | 55.0% | |
| AGGTTGTAGCACCACCAGAG+AGG | + | chr7.4:15595160-15595179 | MS.gene017992:CDS | 55.0% | |
| CCAGCCATCCATGATAGGGA+TGG | + | chr7.4:15594963-15594982 | MS.gene017992:CDS | 55.0% | |
| CCATCCCTATCATGGATGGC+TGG | - | chr7.4:15594966-15594985 | None:intergenic | 55.0% | |
| CGATGTCCGATGTTGGACAC+CGG | - | chr7.4:15593742-15593761 | None:intergenic | 55.0% | |
| CGGTGTCCAACATCGGACAT+CGG | + | chr7.4:15593740-15593759 | MS.gene017992:intron | 55.0% | |
| CGTGGTCGTCCTACTATGCT+AGG | - | chr7.4:15593927-15593946 | None:intergenic | 55.0% | |
| CTGCCCATCAGGTAGCTCAT+AGG | - | chr7.4:15594896-15594915 | None:intergenic | 55.0% | |
| TAGGACGACCACGTCACACA+GGG | + | chr7.4:15593934-15593953 | MS.gene017992:CDS | 55.0% | |
| TCGTGTCCGGTGTCCAACAT+CGG | + | chr7.4:15593733-15593752 | MS.gene017992:intron | 55.0% | |
| ! | CGTCACACAGGGGTAATGGT+TGG | + | chr7.4:15593945-15593964 | MS.gene017992:CDS | 55.0% |
| ! | CTATGAGCTACCTGATGGGC+AGG | + | chr7.4:15594894-15594913 | MS.gene017992:CDS | 55.0% |
| ! | TACGTGCCGATGTCCGATGT+TGG | - | chr7.4:15593749-15593768 | None:intergenic | 55.0% |
| AGGACGACCACGTCACACAG+GGG | + | chr7.4:15593935-15593954 | MS.gene017992:CDS | 60.0% | |
| CAACGGAACCGGCATGGTCA+AGG | + | chr7.4:15593653-15593672 | MS.gene017992:CDS | 60.0% | |
| GTAGGACGACCACGTCACAC+AGG | + | chr7.4:15593933-15593952 | MS.gene017992:CDS | 60.0% | |
| TTGCGACAACGGAACCGGCA+TGG | + | chr7.4:15593647-15593666 | MS.gene017992:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 15593615 | 15595478 | 15593615 | ID=MS.gene017992 |
| chr7.4 | mRNA | 15593615 | 15595478 | 15593615 | ID=MS.gene017992.t1;Parent=MS.gene017992 |
| chr7.4 | exon | 15593615 | 15593674 | 15593615 | ID=MS.gene017992.t1.exon1;Parent=MS.gene017992.t1 |
| chr7.4 | CDS | 15593615 | 15593674 | 15593615 | ID=cds.MS.gene017992.t1;Parent=MS.gene017992.t1 |
| chr7.4 | exon | 15593885 | 15594278 | 15593885 | ID=MS.gene017992.t1.exon2;Parent=MS.gene017992.t1 |
| chr7.4 | CDS | 15593885 | 15594278 | 15593885 | ID=cds.MS.gene017992.t1;Parent=MS.gene017992.t1 |
| chr7.4 | exon | 15594626 | 15595239 | 15594626 | ID=MS.gene017992.t1.exon3;Parent=MS.gene017992.t1 |
| chr7.4 | CDS | 15594626 | 15595239 | 15594626 | ID=cds.MS.gene017992.t1;Parent=MS.gene017992.t1 |
| chr7.4 | exon | 15595413 | 15595478 | 15595413 | ID=MS.gene017992.t1.exon4;Parent=MS.gene017992.t1 |
| chr7.4 | CDS | 15595413 | 15595478 | 15595413 | ID=cds.MS.gene017992.t1;Parent=MS.gene017992.t1 |
| Gene Sequence |
| Protein sequence |
