Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055991.t1 | XP_003625265.1 | 100 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 4.80E-217 | 763.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055991.t1 | P46258 | 98.9 | 377 | 4 | 0 | 1 | 377 | 1 | 377 | 1.8e-217 | 756.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055991.t1 | G7KVE1 | 100.0 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 3.4e-217 | 763.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055991 | MS.gene34057 | 0.80406 | 2.59E-49 | -1.69E-46 |
| MS.gene055991 | MS.gene41692 | 0.816806 | 4.57E-52 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055991.t1 | MTR_7g093260 | 100.000 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 0.0 | 785 |
| MS.gene055991.t1 | MTR_0015s0130 | 96.817 | 377 | 12 | 0 | 1 | 377 | 1 | 377 | 0.0 | 769 |
| MS.gene055991.t1 | MTR_2g096840 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 765 |
| MS.gene055991.t1 | MTR_6g004000 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene055991.t1 | MTR_3g095530 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene055991.t1 | MTR_7g026230 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene055991.t1 | MTR_2g008050 | 92.838 | 377 | 27 | 0 | 1 | 377 | 1 | 377 | 0.0 | 744 |
| MS.gene055991.t1 | MTR_7g027185 | 43.799 | 379 | 199 | 3 | 10 | 374 | 7 | 385 | 2.01e-120 | 354 |
| MS.gene055991.t1 | MTR_7g027185 | 45.070 | 355 | 182 | 2 | 10 | 351 | 7 | 361 | 9.32e-118 | 346 |
| MS.gene055991.t1 | MTR_7g027185 | 48.208 | 307 | 157 | 1 | 10 | 314 | 7 | 313 | 3.31e-110 | 325 |
| MS.gene055991.t1 | MTR_5g066400 | 37.136 | 447 | 207 | 12 | 1 | 376 | 1 | 444 | 1.61e-91 | 282 |
| MS.gene055991.t1 | MTR_8g464900 | 36.343 | 432 | 202 | 11 | 1 | 362 | 1 | 429 | 1.01e-84 | 264 |
| MS.gene055991.t1 | MTR_5g066400 | 34.529 | 446 | 192 | 12 | 1 | 376 | 1 | 416 | 9.86e-79 | 248 |
| MS.gene055991.t1 | MTR_8g089630 | 34.845 | 419 | 207 | 10 | 10 | 372 | 10 | 418 | 9.57e-72 | 230 |
| MS.gene055991.t1 | MTR_3g030550 | 37.766 | 376 | 208 | 9 | 11 | 377 | 5 | 363 | 2.48e-68 | 219 |
| MS.gene055991.t1 | MTR_5g066400 | 34.000 | 400 | 193 | 11 | 1 | 332 | 1 | 397 | 1.62e-67 | 219 |
| MS.gene055991.t1 | MTR_2g445210 | 27.869 | 427 | 242 | 7 | 4 | 366 | 2 | 426 | 3.20e-55 | 188 |
| MS.gene055991.t1 | MTR_8g007440 | 33.588 | 262 | 151 | 6 | 77 | 319 | 9 | 266 | 8.45e-45 | 155 |
| MS.gene055991.t1 | MTR_2g445210 | 26.330 | 376 | 211 | 7 | 4 | 315 | 2 | 375 | 2.80e-41 | 149 |
| MS.gene055991.t1 | MTR_3g010170 | 30.303 | 297 | 183 | 9 | 91 | 371 | 179 | 467 | 1.47e-39 | 146 |
| MS.gene055991.t1 | MTR_8g070520 | 31.058 | 293 | 175 | 10 | 91 | 366 | 187 | 469 | 2.41e-35 | 135 |
| MS.gene055991.t1 | MTR_8g070520 | 30.576 | 278 | 167 | 9 | 91 | 352 | 187 | 454 | 4.40e-32 | 126 |
| MS.gene055991.t1 | MTR_3g067945 | 29.762 | 252 | 158 | 7 | 9 | 245 | 21 | 268 | 3.59e-23 | 101 |
| MS.gene055991.t1 | MTR_3g067945 | 34.426 | 122 | 77 | 2 | 250 | 368 | 586 | 707 | 8.52e-16 | 79.3 |
| MS.gene055991.t1 | MTR_8g008540 | 70.270 | 37 | 11 | 0 | 340 | 376 | 41 | 77 | 4.15e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055991.t1 | AT5G09810 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MS.gene055991.t1 | AT3G12110 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 760 |
| MS.gene055991.t1 | AT3G46520 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT3G53750 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT3G53750 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT3G46520 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT2G37620 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene055991.t1 | AT5G59370 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 758 |
| MS.gene055991.t1 | AT5G59370 | 95.491 | 377 | 17 | 0 | 1 | 377 | 1 | 377 | 0.0 | 758 |
| MS.gene055991.t1 | AT3G18780 | 90.981 | 377 | 34 | 0 | 1 | 377 | 1 | 377 | 0.0 | 737 |
| MS.gene055991.t1 | AT3G18780 | 90.981 | 377 | 34 | 0 | 1 | 377 | 1 | 377 | 0.0 | 737 |
| MS.gene055991.t1 | AT1G49240 | 90.716 | 377 | 35 | 0 | 1 | 377 | 1 | 377 | 0.0 | 735 |
| MS.gene055991.t1 | AT3G18780 | 90.808 | 359 | 33 | 0 | 1 | 359 | 1 | 359 | 0.0 | 696 |
| MS.gene055991.t1 | AT2G42100 | 85.326 | 368 | 54 | 0 | 10 | 377 | 11 | 378 | 0.0 | 677 |
| MS.gene055991.t1 | AT2G42090 | 71.351 | 370 | 101 | 1 | 7 | 376 | 1 | 365 | 0.0 | 568 |
| MS.gene055991.t1 | AT2G42170 | 78.313 | 332 | 69 | 1 | 46 | 377 | 1 | 329 | 0.0 | 560 |
| MS.gene055991.t1 | AT2G42170 | 78.313 | 332 | 69 | 1 | 46 | 377 | 1 | 329 | 0.0 | 560 |
| MS.gene055991.t1 | AT2G42170 | 78.205 | 312 | 65 | 1 | 46 | 357 | 1 | 309 | 0.0 | 522 |
| MS.gene055991.t1 | AT2G42170 | 78.205 | 312 | 65 | 1 | 46 | 357 | 1 | 309 | 0.0 | 522 |
| MS.gene055991.t1 | AT2G42170 | 78.761 | 226 | 45 | 1 | 152 | 377 | 9 | 231 | 5.79e-132 | 377 |
| MS.gene055991.t1 | AT3G27000 | 44.591 | 379 | 196 | 4 | 10 | 374 | 7 | 385 | 2.61e-122 | 358 |
| MS.gene055991.t1 | AT1G18450 | 36.569 | 443 | 211 | 9 | 1 | 376 | 1 | 440 | 7.98e-94 | 288 |
| MS.gene055991.t1 | AT1G13180 | 34.615 | 416 | 212 | 11 | 10 | 372 | 9 | 417 | 7.01e-72 | 231 |
| MS.gene055991.t1 | AT3G60830 | 37.731 | 379 | 212 | 9 | 7 | 377 | 1 | 363 | 1.19e-67 | 218 |
| MS.gene055991.t1 | AT3G33520 | 30.166 | 421 | 236 | 7 | 10 | 377 | 5 | 420 | 6.97e-59 | 197 |
| MS.gene055991.t1 | AT5G56180 | 30.986 | 284 | 171 | 9 | 91 | 358 | 50 | 324 | 2.21e-34 | 130 |
| MS.gene055991.t1 | AT5G56180 | 30.986 | 284 | 171 | 9 | 91 | 358 | 182 | 456 | 1.20e-33 | 130 |
| MS.gene055991.t1 | AT3G12380 | 31.395 | 258 | 152 | 9 | 9 | 245 | 21 | 274 | 8.24e-26 | 109 |
| MS.gene055991.t1 | AT3G12380 | 33.103 | 145 | 93 | 2 | 228 | 368 | 572 | 716 | 3.20e-16 | 80.9 |
| MS.gene055991.t1 | AT3G12380 | 31.250 | 256 | 149 | 9 | 9 | 245 | 21 | 268 | 3.66e-25 | 107 |
| MS.gene055991.t1 | AT3G12380 | 33.103 | 145 | 93 | 2 | 228 | 368 | 566 | 710 | 3.22e-16 | 80.9 |
| MS.gene055991.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.58e-23 | 95.1 |
| MS.gene055991.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.58e-23 | 95.1 |
| MS.gene055991.t1 | AT5G56180 | 32.292 | 192 | 110 | 7 | 91 | 271 | 182 | 364 | 8.79e-19 | 87.4 |
| MS.gene055991.t1 | AT1G73910 | 29.268 | 123 | 71 | 2 | 1 | 107 | 1 | 123 | 3.24e-12 | 64.7 |
Find 106 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGGTATTGTTCTGGATTC+TGG | 0.149133 | 7.1:+14264051 | MS.gene055991:intron |
| AGCATGGGATTGTTAGTAAT+TGG | 0.164954 | 7.1:+14263474 | MS.gene055991:CDS |
| CTGGATTGGAGGTTCCATTT+TGG | 0.195803 | 7.1:+14264623 | MS.gene055991:CDS |
| AAGGGATATGAAGGAGAAAT+TGG | 0.274984 | 7.1:+14264233 | MS.gene055991:CDS |
| TGTTAGTAATTGGGATGATA+TGG | 0.286824 | 7.1:+14263484 | MS.gene055991:CDS |
| AGAAGAACAGGGTGTTCTTC+AGG | 0.300324 | 7.1:-14263551 | None:intergenic |
| GATGGCTGGAACAAAACTTC+TGG | 0.305788 | 7.1:-14264378 | None:intergenic |
| CATTTCCTGTGTACTATTGA+TGG | 0.320989 | 7.1:-14264878 | None:intergenic |
| TCCCCATGCAATCCTTCGTT+TGG | 0.323983 | 7.1:+14264119 | MS.gene055991:CDS |
| ATCCTTCGTTTGGATCTTGC+AGG | 0.332165 | 7.1:+14264129 | MS.gene055991:CDS |
| CTAACAATCCCATGCTCAAT+TGG | 0.332451 | 7.1:-14263467 | None:intergenic |
| GGTGCTTCAGTGAGAAGAAC+AGG | 0.346523 | 7.1:-14263563 | None:intergenic |
| AGTGCATATCCCTCGTAAAT+AGG | 0.354141 | 7.1:-14264096 | None:intergenic |
| AAGGCAGAATATGATGAATC+TGG | 0.369605 | 7.1:+14264854 | MS.gene055991:CDS |
| TAGGTATTGTTCTGGATTCT+GGG | 0.386484 | 7.1:+14264052 | MS.gene055991:intron |
| AGTGCTGAGGGATGCCAAAA+TGG | 0.398050 | 7.1:-14264637 | None:intergenic |
| CTGCCCATCAGGTAGCTCAT+AGG | 0.415209 | 7.1:-14264322 | None:intergenic |
| CGATTAGCCTTAGGATTAAG+AGG | 0.422207 | 7.1:-14263584 | None:intergenic |
| ATCCCTCAGCACTTTCCAAC+AGG | 0.435296 | 7.1:+14264647 | MS.gene055991:CDS |
| GGTTCCGTTGTCGCAAACTA+AGG | 0.435984 | 7.1:-14263062 | None:intergenic |
| TATGCGAGTGGTCGTACAAC+TGG | 0.440352 | 7.1:+14263686 | MS.gene055991:CDS |
| GCAGTATTCCCTAGCATAGT+AGG | 0.445310 | 7.1:+14263344 | MS.gene055991:CDS |
| CAGCAGAGCGAGAAATTGTA+AGG | 0.445586 | 7.1:+14264214 | MS.gene055991:CDS |
| GCATGGGATTGTTAGTAATT+GGG | 0.447989 | 7.1:+14263475 | MS.gene055991:CDS |
| AGGAGATTCCATCCCGATCA+TGG | 0.450520 | 7.1:-14264400 | None:intergenic |
| TGTTCCAGCCATCCATGATC+GGG | 0.451385 | 7.1:+14264388 | MS.gene055991:CDS |
| ATCGTGATTACCTGCCCATC+AGG | 0.459221 | 7.1:-14264333 | None:intergenic |
| CTCATCCTATCAGCAATACC+AGG | 0.462364 | 7.1:-14264525 | None:intergenic |
| AGAAATTGTAAGGGATATGA+AGG | 0.463583 | 7.1:+14264224 | MS.gene055991:CDS |
| TTAGTTTGCGACAACGGAAC+CGG | 0.471674 | 7.1:+14263064 | MS.gene055991:CDS |
| CAGAGAGGAAGTACAGTGTC+TGG | 0.471867 | 7.1:+14264604 | MS.gene055991:CDS |
| ATGGCTGGAACAAAACTTCT+GGG | 0.473579 | 7.1:-14264377 | None:intergenic |
| TATTGCTCTTGATTATGAGC+AGG | 0.475672 | 7.1:+14264260 | MS.gene055991:CDS |
| GACATTAGGAAGGATCTCTA+TGG | 0.477623 | 7.1:+14264468 | MS.gene055991:CDS |
| GGGATGATATGGAGAAGATC+TGG | 0.484705 | 7.1:+14263495 | MS.gene055991:CDS |
| AGGAAGTACAGTGTCTGGAT+TGG | 0.485496 | 7.1:+14264609 | MS.gene055991:CDS |
| ATTGTTCTGGATTCTGGGGA+TGG | 0.491654 | 7.1:+14264057 | MS.gene055991:CDS |
| CAGACACTGTACTTCCTCTC+TGG | 0.493244 | 7.1:-14264603 | None:intergenic |
| CTATGAGCTACCTGATGGGC+AGG | 0.495402 | 7.1:+14264323 | MS.gene055991:CDS |
| CACAGGGGTAATGGTTGGAA+TGG | 0.495803 | 7.1:+14263379 | MS.gene055991:CDS |
| TAACAATCCCATGCTCAATT+GGG | 0.497649 | 7.1:-14263466 | None:intergenic |
| AAGTACAGTGTCTGGATTGG+AGG | 0.501277 | 7.1:+14264612 | MS.gene055991:CDS |
| TGTTCCCTGGTATTGCTGAT+AGG | 0.501884 | 7.1:+14264520 | MS.gene055991:CDS |
| GATCCAAACGAAGGATTGCA+TGG | 0.503842 | 7.1:-14264122 | None:intergenic |
| TGAAGTGTGATGTTGACATT+AGG | 0.508969 | 7.1:+14264454 | MS.gene055991:CDS |
| TCCACGTCACACAGGGGTAA+TGG | 0.512712 | 7.1:+14263370 | MS.gene055991:CDS |
| TTGTTCCAGCCATCCATGAT+CGG | 0.512717 | 7.1:+14264387 | MS.gene055991:CDS |
| CGTGGACGTCCTACTATGCT+AGG | 0.514609 | 7.1:-14263353 | None:intergenic |
| AATTTCTCGCTCTGCTGAAG+TGG | 0.516029 | 7.1:-14264208 | None:intergenic |
| CCATCCCGATCATGGATGGC+TGG | 0.516535 | 7.1:-14264392 | None:intergenic |
| ACAGGGGTAATGGTTGGAAT+GGG | 0.516861 | 7.1:+14263380 | MS.gene055991:CDS |
| TTGAAATACCCAATTGAGCA+TGG | 0.529351 | 7.1:+14263458 | MS.gene055991:CDS |
| TGCAGGAGATGATGCACCAA+GGG | 0.547290 | 7.1:+14263322 | MS.gene055991:CDS |
| GAGCAAGGAGATTACAGCAT+TGG | 0.550032 | 7.1:+14264545 | MS.gene055991:CDS |
| TTGAGTTAAATGTTGTCTCA+TGG | 0.551265 | 7.1:-14264428 | None:intergenic |
| CAGCCCTTAGTTTGCGACAA+CGG | 0.557180 | 7.1:+14263058 | MS.gene055991:CDS |
| TCTTGATTATGAGCAGGAGT+TGG | 0.557428 | 7.1:+14264266 | MS.gene055991:CDS |
| TGAATAGCAACATACATAGC+AGG | 0.559953 | 7.1:-14263647 | None:intergenic |
| GTGCTTTCGCTTTATGCGAG+TGG | 0.560364 | 7.1:+14263674 | MS.gene055991:CDS |
| GGAACAAAACTTCTGGGCAT+CGG | 0.561285 | 7.1:-14264371 | None:intergenic |
| AGGCAGAATATGATGAATCT+GGG | 0.564408 | 7.1:+14264855 | MS.gene055991:CDS |
| TGAAGCACCTCTTAATCCTA+AGG | 0.566737 | 7.1:+14263577 | MS.gene055991:CDS |
| ATCCAAACGAAGGATTGCAT+GGG | 0.568962 | 7.1:-14264121 | None:intergenic |
| CAACGGAACCGGCATGGTCA+AGG | 0.570249 | 7.1:+14263075 | MS.gene055991:CDS |
| AATGTTGTCTCATGGATACC+AGG | 0.572742 | 7.1:-14264420 | None:intergenic |
| ACCTAGTAGCATGAAAATCA+AGG | 0.573048 | 7.1:+14264569 | MS.gene055991:CDS |
| GGTGAAAGTGTAGCCACGCT+CGG | 0.575757 | 7.1:-14264187 | None:intergenic |
| TGAAATACCCAATTGAGCAT+GGG | 0.576183 | 7.1:+14263459 | MS.gene055991:CDS |
| ATGCTAGGGAATACTGCCCT+TGG | 0.578562 | 7.1:-14263338 | None:intergenic |
| ACCATTACCCCTGTGTGACG+TGG | 0.580899 | 7.1:-14263371 | None:intergenic |
| GGTGGTTCAACAATGTTCCC+TGG | 0.583355 | 7.1:+14264507 | MS.gene055991:CDS |
| CGTCACACAGGGGTAATGGT+TGG | 0.583763 | 7.1:+14263374 | MS.gene055991:CDS |
| GATTCCATCCCGATCATGGA+TGG | 0.583952 | 7.1:-14264396 | None:intergenic |
| GTTCCGTTGTCGCAAACTAA+GGG | 0.584635 | 7.1:-14263061 | None:intergenic |
| TATGGTAACATTGTGTTGAG+TGG | 0.586496 | 7.1:+14264486 | MS.gene055991:CDS |
| GATTGATACCTTGACCATGC+CGG | 0.591606 | 7.1:-14263083 | None:intergenic |
| GGTAACATTGTGTTGAGTGG+TGG | 0.592071 | 7.1:+14264489 | MS.gene055991:CDS |
| TTACCTGTTGGAAAGTGCTG+AGG | 0.592935 | 7.1:-14264650 | None:intergenic |
| AAAACCTATGAGCTACCTGA+TGG | 0.593395 | 7.1:+14264318 | MS.gene055991:CDS |
| ATGATCGGGATGGAATCTCC+TGG | 0.598231 | 7.1:+14264402 | MS.gene055991:CDS |
| TAGGACGTCCACGTCACACA+GGG | 0.610133 | 7.1:+14263363 | MS.gene055991:CDS |
| AAACCTATGAGCTACCTGAT+GGG | 0.615605 | 7.1:+14264319 | MS.gene055991:CDS |
| ACACTGTACTTCCTCTCTGG+TGG | 0.626941 | 7.1:-14264600 | None:intergenic |
| TTGCGACAACGGAACCGGCA+TGG | 0.628011 | 7.1:+14263069 | MS.gene055991:CDS |
| AGGTATTGTTCTGGATTCTG+GGG | 0.628541 | 7.1:+14264053 | MS.gene055991:intron |
| GATGGGCAGGTAATCACGAT+CGG | 0.630520 | 7.1:+14264336 | MS.gene055991:CDS |
| CCAGCCATCCATGATCGGGA+TGG | 0.632027 | 7.1:+14264392 | MS.gene055991:CDS |
| TGGGGATGGTGTTAGTCACA+CGG | 0.632223 | 7.1:+14264071 | MS.gene055991:CDS |
| GTGGACGTCCTACTATGCTA+GGG | 0.634198 | 7.1:-14263352 | None:intergenic |
| TATTGCTGATAGGATGAGCA+AGG | 0.645350 | 7.1:+14264530 | MS.gene055991:CDS |
| CTGGGCCATCAATAGTACAC+AGG | 0.648814 | 7.1:+14264873 | MS.gene055991:CDS |
| TACCTGTTGGAAAGTGCTGA+GGG | 0.649811 | 7.1:-14264649 | None:intergenic |
| GTAGGACGTCCACGTCACAC+AGG | 0.654094 | 7.1:+14263362 | MS.gene055991:CDS |
| TGTGCAGATGTGGATTGCAA+AGG | 0.658163 | 7.1:+14264835 | MS.gene055991:intron |
| GATGAAGCACAATCAAAGAG+AGG | 0.658995 | 7.1:+14263425 | MS.gene055991:CDS |
| CACACGGTTCCTATTTACGA+GGG | 0.661422 | 7.1:+14264087 | MS.gene055991:CDS |
| AGCAGAGCGAGAAATTGTAA+GGG | 0.663335 | 7.1:+14264215 | MS.gene055991:CDS |
| TTGCAGGAGATGATGCACCA+AGG | 0.664250 | 7.1:+14263321 | MS.gene055991:CDS |
| TCACACGGTTCCTATTTACG+AGG | 0.670052 | 7.1:+14264086 | MS.gene055991:CDS |
| GTGCTTCAGTGAGAAGAACA+GGG | 0.670285 | 7.1:-14263562 | None:intergenic |
| TCCAAACGAAGGATTGCATG+GGG | 0.676900 | 7.1:-14264120 | None:intergenic |
| GTGTGATGTTGACATTAGGA+AGG | 0.677606 | 7.1:+14264458 | MS.gene055991:CDS |
| GACCTGCAAGATCCAAACGA+AGG | 0.699076 | 7.1:-14264131 | None:intergenic |
| AGGTTGTAGCACCACCAGAG+AGG | 0.701742 | 7.1:+14264589 | MS.gene055991:CDS |
| TCATCCTATCAGCAATACCA+GGG | 0.706641 | 7.1:-14264524 | None:intergenic |
| AGGACGTCCACGTCACACAG+GGG | 0.764581 | 7.1:+14263364 | MS.gene055991:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCCATTTTTTAAATTATTAC+CGG | - | chr7.1:14263844-14263863 | None:intergenic | 15.0% |
| !! | ACCGGTAATAATTTAAAAAA+TGG | + | chr7.1:14263840-14263859 | MS.gene055991:intron | 20.0% |
| !!! | CTTTCTATTGTTATTGTTTT+AGG | + | chr7.1:14263292-14263311 | MS.gene055991:intron | 20.0% |
| ! | AAGTACAGGTTATAAAAAGT+AGG | + | chr7.1:14264733-14264752 | MS.gene055991:intron | 25.0% |
| ! | GAATGATGAATATGATTTGA+AGG | + | chr7.1:14263959-14263978 | MS.gene055991:intron | 25.0% |
| ! | GATAACTCAGTAATTAAATC+AGG | + | chr7.1:14264692-14264711 | MS.gene055991:intron | 25.0% |
| ! | TGATTCATACAAAACTCATA+CGG | - | chr7.1:14263787-14263806 | None:intergenic | 25.0% |
| ! | TTCATTTCAACTATAAGTAC+AGG | + | chr7.1:14264719-14264738 | MS.gene055991:intron | 25.0% |
| AATCCATCAAATTTACCTGT+TGG | - | chr7.1:14264665-14264684 | None:intergenic | 30.0% | |
| AGAAATTGTAAGGGATATGA+AGG | + | chr7.1:14264224-14264243 | MS.gene055991:CDS | 30.0% | |
| TACAGGTTATAAAAAGTAGG+AGG | + | chr7.1:14264736-14264755 | MS.gene055991:intron | 30.0% | |
| TGTTAGTAATTGGGATGATA+TGG | + | chr7.1:14263484-14263503 | MS.gene055991:CDS | 30.0% | |
| TTGAGTTAAATGTTGTCTCA+TGG | - | chr7.1:14264431-14264450 | None:intergenic | 30.0% | |
| TTTCCAACAGGTAAATTTGA+TGG | + | chr7.1:14264659-14264678 | MS.gene055991:intron | 30.0% | |
| ! | TGATTTTCTGTGTTTTGTGT+AGG | + | chr7.1:14264033-14264052 | MS.gene055991:intron | 30.0% |
| !!! | CTATTGTTATTGTTTTAGGC+TGG | + | chr7.1:14263296-14263315 | MS.gene055991:intron | 30.0% |
| AAGGCAGAATATGATGAATC+TGG | + | chr7.1:14264854-14264873 | MS.gene055991:CDS | 35.0% | |
| AAGGGATATGAAGGAGAAAT+TGG | + | chr7.1:14264233-14264252 | MS.gene055991:CDS | 35.0% | |
| ACCTAGTAGCATGAAAATCA+AGG | + | chr7.1:14264569-14264588 | MS.gene055991:CDS | 35.0% | |
| AGCATGGGATTGTTAGTAAT+TGG | + | chr7.1:14263474-14263493 | MS.gene055991:CDS | 35.0% | |
| AGGCAGAATATGATGAATCT+GGG | + | chr7.1:14264855-14264874 | MS.gene055991:CDS | 35.0% | |
| CAAGATTGAAGGATAAACAC+CGG | + | chr7.1:14263822-14263841 | MS.gene055991:intron | 35.0% | |
| CATTTCCTGTGTACTATTGA+TGG | - | chr7.1:14264881-14264900 | None:intergenic | 35.0% | |
| GAATGTAATCACATATGTCG+TGG | + | chr7.1:14263879-14263898 | MS.gene055991:intron | 35.0% | |
| GCATGGGATTGTTAGTAATT+GGG | + | chr7.1:14263475-14263494 | MS.gene055991:CDS | 35.0% | |
| GGACAAAAAGATGCATATGT+TGG | + | chr7.1:14263401-14263420 | MS.gene055991:CDS | 35.0% | |
| TAACAATCCCATGCTCAATT+GGG | - | chr7.1:14263469-14263488 | None:intergenic | 35.0% | |
| TATGGTAACATTGTGTTGAG+TGG | + | chr7.1:14264486-14264505 | MS.gene055991:CDS | 35.0% | |
| TATTGCTCTTGATTATGAGC+AGG | + | chr7.1:14264260-14264279 | MS.gene055991:CDS | 35.0% | |
| TGAAATACCCAATTGAGCAT+GGG | + | chr7.1:14263459-14263478 | MS.gene055991:CDS | 35.0% | |
| TGAAGTGTGATGTTGACATT+AGG | + | chr7.1:14264454-14264473 | MS.gene055991:CDS | 35.0% | |
| TGAATAGCAACATACATAGC+AGG | - | chr7.1:14263650-14263669 | None:intergenic | 35.0% | |
| TTGAAATACCCAATTGAGCA+TGG | + | chr7.1:14263458-14263477 | MS.gene055991:CDS | 35.0% | |
| ! | ACCTTGATTTTCATGCTACT+AGG | - | chr7.1:14264573-14264592 | None:intergenic | 35.0% |
| ! | TAGGTATTGTTCTGGATTCT+GGG | + | chr7.1:14264052-14264071 | MS.gene055991:intron | 35.0% |
| !! | GTTTTGTGTAGGTATTGTTC+TGG | + | chr7.1:14264044-14264063 | MS.gene055991:intron | 35.0% |
| !!! | ATTTTCTCACGATTAGCCTT+AGG | - | chr7.1:14263596-14263615 | None:intergenic | 35.0% |
| !!! | TACTTTTTCTTGTGCAGATG+TGG | + | chr7.1:14264825-14264844 | MS.gene055991:intron | 35.0% |
| AAAACCTATGAGCTACCTGA+TGG | + | chr7.1:14264318-14264337 | MS.gene055991:CDS | 40.0% | |
| AAACCTATGAGCTACCTGAT+GGG | + | chr7.1:14264319-14264338 | MS.gene055991:CDS | 40.0% | |
| AATGTTGTCTCATGGATACC+AGG | - | chr7.1:14264423-14264442 | None:intergenic | 40.0% | |
| AGCAGAGCGAGAAATTGTAA+GGG | + | chr7.1:14264215-14264234 | MS.gene055991:CDS | 40.0% | |
| AGTGCATATCCCTCGTAAAT+AGG | - | chr7.1:14264099-14264118 | None:intergenic | 40.0% | |
| ATCCAAACGAAGGATTGCAT+GGG | - | chr7.1:14264124-14264143 | None:intergenic | 40.0% | |
| ATGGCTGGAACAAAACTTCT+GGG | - | chr7.1:14264380-14264399 | None:intergenic | 40.0% | |
| CGGTGTTTATCCTTCAATCT+TGG | - | chr7.1:14263824-14263843 | None:intergenic | 40.0% | |
| CTAACAATCCCATGCTCAAT+TGG | - | chr7.1:14263470-14263489 | None:intergenic | 40.0% | |
| GACATTAGGAAGGATCTCTA+TGG | + | chr7.1:14264468-14264487 | MS.gene055991:CDS | 40.0% | |
| GATGAAGCACAATCAAAGAG+AGG | + | chr7.1:14263425-14263444 | MS.gene055991:CDS | 40.0% | |
| GCCATGTGATGATACAAACT+AGG | + | chr7.1:14263986-14264005 | MS.gene055991:intron | 40.0% | |
| GTGTCTGCATCTCTGTTTAA+TGG | + | chr7.1:14263263-14263282 | MS.gene055991:intron | 40.0% | |
| GTGTGATGTTGACATTAGGA+AGG | + | chr7.1:14264458-14264477 | MS.gene055991:CDS | 40.0% | |
| TATTGCTGATAGGATGAGCA+AGG | + | chr7.1:14264530-14264549 | MS.gene055991:CDS | 40.0% | |
| TCATCCTATCAGCAATACCA+GGG | - | chr7.1:14264527-14264546 | None:intergenic | 40.0% | |
| TCTTGATTATGAGCAGGAGT+TGG | + | chr7.1:14264266-14264285 | MS.gene055991:CDS | 40.0% | |
| TGTAATCACATATGTCGTGG+TGG | + | chr7.1:14263882-14263901 | MS.gene055991:intron | 40.0% | |
| TTAAACAGAGATGCAGACAC+CGG | - | chr7.1:14263263-14263282 | None:intergenic | 40.0% | |
| ! | AGGTATTGTTCTGGATTCTG+GGG | + | chr7.1:14264053-14264072 | MS.gene055991:intron | 40.0% |
| ! | AGTATGTAGCATCGAAACGT+TGG | - | chr7.1:14263929-14263948 | None:intergenic | 40.0% |
| ! | GCCTAGTTTGTATCATCACA+TGG | - | chr7.1:14263990-14264009 | None:intergenic | 40.0% |
| ! | GTAGGTATTGTTCTGGATTC+TGG | + | chr7.1:14264051-14264070 | MS.gene055991:intron | 40.0% |
| ! | TGAAGCACCTCTTAATCCTA+AGG | + | chr7.1:14263577-14263596 | MS.gene055991:CDS | 40.0% |
| !! | ATGAAGATTTTAACCGAGCG+TGG | + | chr7.1:14264174-14264193 | MS.gene055991:CDS | 40.0% |
| !! | CGATTAGCCTTAGGATTAAG+AGG | - | chr7.1:14263587-14263606 | None:intergenic | 40.0% |
| !! | GAGCTTTTGATCAGAAGTGT+TGG | - | chr7.1:14263136-14263155 | None:intergenic | 40.0% |
| !!! | TTGTTTTAGGCTGGTTTTGC+AGG | + | chr7.1:14263305-14263324 | MS.gene055991:intron | 40.0% |
| AATGTCCGACACTGATCTGT+CGG | - | chr7.1:14263170-14263189 | None:intergenic | 45.0% | |
| AATTTCTCGCTCTGCTGAAG+TGG | - | chr7.1:14264211-14264230 | None:intergenic | 45.0% | |
| ACACACGACACCAAGATTGA+AGG | + | chr7.1:14263811-14263830 | MS.gene055991:intron | 45.0% | |
| ATCCTTCGTTTGGATCTTGC+AGG | + | chr7.1:14264129-14264148 | MS.gene055991:CDS | 45.0% | |
| CACACGGTTCCTATTTACGA+GGG | + | chr7.1:14264087-14264106 | MS.gene055991:CDS | 45.0% | |
| CAGCAGAGCGAGAAATTGTA+AGG | + | chr7.1:14264214-14264233 | MS.gene055991:CDS | 45.0% | |
| CTCATCCTATCAGCAATACC+AGG | - | chr7.1:14264528-14264547 | None:intergenic | 45.0% | |
| GAGCAAGGAGATTACAGCAT+TGG | + | chr7.1:14264545-14264564 | MS.gene055991:CDS | 45.0% | |
| GATCCAAACGAAGGATTGCA+TGG | - | chr7.1:14264125-14264144 | None:intergenic | 45.0% | |
| GATGGCTGGAACAAAACTTC+TGG | - | chr7.1:14264381-14264400 | None:intergenic | 45.0% | |
| GATTGATACCTTGACCATGC+CGG | - | chr7.1:14263086-14263105 | None:intergenic | 45.0% | |
| GCAGTATTCCCTAGCATAGT+AGG | + | chr7.1:14263344-14263363 | MS.gene055991:CDS | 45.0% | |
| GGAACAAAACTTCTGGGCAT+CGG | - | chr7.1:14264374-14264393 | None:intergenic | 45.0% | |
| GGGATGATATGGAGAAGATC+TGG | + | chr7.1:14263495-14263514 | MS.gene055991:CDS | 45.0% | |
| GGTAACATTGTGTTGAGTGG+TGG | + | chr7.1:14264489-14264508 | MS.gene055991:CDS | 45.0% | |
| GTGCTTCAGTGAGAAGAACA+GGG | - | chr7.1:14263565-14263584 | None:intergenic | 45.0% | |
| GTTCCGTTGTCGCAAACTAA+GGG | - | chr7.1:14263064-14263083 | None:intergenic | 45.0% | |
| TACGACACTGACACATCACA+CGG | - | chr7.1:14263239-14263258 | None:intergenic | 45.0% | |
| TCACACGGTTCCTATTTACG+AGG | + | chr7.1:14264086-14264105 | MS.gene055991:CDS | 45.0% | |
| TCCAAACGAAGGATTGCATG+GGG | - | chr7.1:14264123-14264142 | None:intergenic | 45.0% | |
| TCTGATCAAAAGCTCGTGTC+CGG | + | chr7.1:14263141-14263160 | MS.gene055991:intron | 45.0% | |
| TGATGTGTCAGTGTCGTATC+CGG | + | chr7.1:14263241-14263260 | MS.gene055991:intron | 45.0% | |
| TGTTCCCTGGTATTGCTGAT+AGG | + | chr7.1:14264520-14264539 | MS.gene055991:CDS | 45.0% | |
| TTGTTCCAGCCATCCATGAT+CGG | + | chr7.1:14264387-14264406 | MS.gene055991:CDS | 45.0% | |
| ! | AAGTACAGTGTCTGGATTGG+AGG | + | chr7.1:14264612-14264631 | MS.gene055991:CDS | 45.0% |
| ! | ACAGGGGTAATGGTTGGAAT+GGG | + | chr7.1:14263380-14263399 | MS.gene055991:CDS | 45.0% |
| ! | AGAAGAACAGGGTGTTCTTC+AGG | - | chr7.1:14263554-14263573 | None:intergenic | 45.0% |
| ! | AGGAAGTACAGTGTCTGGAT+TGG | + | chr7.1:14264609-14264628 | MS.gene055991:CDS | 45.0% |
| ! | ATTGTTCTGGATTCTGGGGA+TGG | + | chr7.1:14264057-14264076 | MS.gene055991:CDS | 45.0% |
| ! | TGTGCAGATGTGGATTGCAA+AGG | + | chr7.1:14264835-14264854 | MS.gene055991:intron | 45.0% |
| !! | CTGGATTGGAGGTTCCATTT+TGG | + | chr7.1:14264623-14264642 | MS.gene055991:CDS | 45.0% |
| !! | TACCTGTTGGAAAGTGCTGA+GGG | - | chr7.1:14264652-14264671 | None:intergenic | 45.0% |
| !! | TTACCTGTTGGAAAGTGCTG+AGG | - | chr7.1:14264653-14264672 | None:intergenic | 45.0% |
| !! | TTAGTTTGCGACAACGGAAC+CGG | + | chr7.1:14263064-14263083 | MS.gene055991:CDS | 45.0% |
| ACACTGTACTTCCTCTCTGG+TGG | - | chr7.1:14264603-14264622 | None:intergenic | 50.0% | |
| AGGAGATTCCATCCCGATCA+TGG | - | chr7.1:14264403-14264422 | None:intergenic | 50.0% | |
| ATCCCTCAGCACTTTCCAAC+AGG | + | chr7.1:14264647-14264666 | MS.gene055991:CDS | 50.0% | |
| ATCGTGATTACCTGCCCATC+AGG | - | chr7.1:14264336-14264355 | None:intergenic | 50.0% | |
| ATGATCGGGATGGAATCTCC+TGG | + | chr7.1:14264402-14264421 | MS.gene055991:CDS | 50.0% | |
| ATGCTAGGGAATACTGCCCT+TGG | - | chr7.1:14263341-14263360 | None:intergenic | 50.0% | |
| CAGACACTGTACTTCCTCTC+TGG | - | chr7.1:14264606-14264625 | None:intergenic | 50.0% | |
| CTGGGCCATCAATAGTACAC+AGG | + | chr7.1:14264873-14264892 | MS.gene055991:CDS | 50.0% | |
| GACCTGCAAGATCCAAACGA+AGG | - | chr7.1:14264134-14264153 | None:intergenic | 50.0% | |
| GATGGGCAGGTAATCACGAT+CGG | + | chr7.1:14264336-14264355 | MS.gene055991:CDS | 50.0% | |
| GATTCCATCCCGATCATGGA+TGG | - | chr7.1:14264399-14264418 | None:intergenic | 50.0% | |
| GGTGCTTCAGTGAGAAGAAC+AGG | - | chr7.1:14263566-14263585 | None:intergenic | 50.0% | |
| GGTGGTTCAACAATGTTCCC+TGG | + | chr7.1:14264507-14264526 | MS.gene055991:CDS | 50.0% | |
| GGTTCCGTTGTCGCAAACTA+AGG | - | chr7.1:14263065-14263084 | None:intergenic | 50.0% | |
| GTGCTTTCGCTTTATGCGAG+TGG | + | chr7.1:14263674-14263693 | MS.gene055991:CDS | 50.0% | |
| GTGGACGTCCTACTATGCTA+GGG | - | chr7.1:14263355-14263374 | None:intergenic | 50.0% | |
| TATGCGAGTGGTCGTACAAC+TGG | + | chr7.1:14263686-14263705 | MS.gene055991:CDS | 50.0% | |
| TCCCCATGCAATCCTTCGTT+TGG | + | chr7.1:14264119-14264138 | MS.gene055991:CDS | 50.0% | |
| TGCAGGAGATGATGCACCAA+GGG | + | chr7.1:14263322-14263341 | MS.gene055991:CDS | 50.0% | |
| TGTTCCAGCCATCCATGATC+GGG | + | chr7.1:14264388-14264407 | MS.gene055991:CDS | 50.0% | |
| TTGCAGGAGATGATGCACCA+AGG | + | chr7.1:14263321-14263340 | MS.gene055991:CDS | 50.0% | |
| ! | AGTGCTGAGGGATGCCAAAA+TGG | - | chr7.1:14264640-14264659 | None:intergenic | 50.0% |
| ! | CACAGGGGTAATGGTTGGAA+TGG | + | chr7.1:14263379-14263398 | MS.gene055991:CDS | 50.0% |
| ! | CAGAGAGGAAGTACAGTGTC+TGG | + | chr7.1:14264604-14264623 | MS.gene055991:CDS | 50.0% |
| ! | TGGGGATGGTGTTAGTCACA+CGG | + | chr7.1:14264071-14264090 | MS.gene055991:CDS | 50.0% |
| !! | CAATCTTGGTGTCGTGTGTC+TGG | - | chr7.1:14263810-14263829 | None:intergenic | 50.0% |
| !! | CAGCCCTTAGTTTGCGACAA+CGG | + | chr7.1:14263058-14263077 | MS.gene055991:CDS | 50.0% |
| ACCATTACCCCTGTGTGACG+TGG | - | chr7.1:14263374-14263393 | None:intergenic | 55.0% | |
| AGGTTGTAGCACCACCAGAG+AGG | + | chr7.1:14264589-14264608 | MS.gene055991:CDS | 55.0% | |
| CGTGGACGTCCTACTATGCT+AGG | - | chr7.1:14263356-14263375 | None:intergenic | 55.0% | |
| CTGCCCATCAGGTAGCTCAT+AGG | - | chr7.1:14264325-14264344 | None:intergenic | 55.0% | |
| GACACTGATCTGTCGGACAC+CGG | - | chr7.1:14263163-14263182 | None:intergenic | 55.0% | |
| GGTGTCCGACAGATCAGTGT+CGG | + | chr7.1:14263162-14263181 | MS.gene055991:intron | 55.0% | |
| TAGGACGTCCACGTCACACA+GGG | + | chr7.1:14263363-14263382 | MS.gene055991:CDS | 55.0% | |
| TCCACGTCACACAGGGGTAA+TGG | + | chr7.1:14263370-14263389 | MS.gene055991:CDS | 55.0% | |
| ! | CGTCACACAGGGGTAATGGT+TGG | + | chr7.1:14263374-14263393 | MS.gene055991:CDS | 55.0% |
| ! | CTATGAGCTACCTGATGGGC+AGG | + | chr7.1:14264323-14264342 | MS.gene055991:CDS | 55.0% |
| ! | GGTGAAAGTGTAGCCACGCT+CGG | - | chr7.1:14264190-14264209 | None:intergenic | 55.0% |
| AGGACGTCCACGTCACACAG+GGG | + | chr7.1:14263364-14263383 | MS.gene055991:CDS | 60.0% | |
| CAACGGAACCGGCATGGTCA+AGG | + | chr7.1:14263075-14263094 | MS.gene055991:CDS | 60.0% | |
| CCAGCCATCCATGATCGGGA+TGG | + | chr7.1:14264392-14264411 | MS.gene055991:CDS | 60.0% | |
| CCATCCCGATCATGGATGGC+TGG | - | chr7.1:14264395-14264414 | None:intergenic | 60.0% | |
| GTAGGACGTCCACGTCACAC+AGG | + | chr7.1:14263362-14263381 | MS.gene055991:CDS | 60.0% | |
| TTGCGACAACGGAACCGGCA+TGG | + | chr7.1:14263069-14263088 | MS.gene055991:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 14263037 | 14264907 | 14263037 | ID=MS.gene055991 |
| chr7.1 | mRNA | 14263037 | 14264907 | 14263037 | ID=MS.gene055991.t1;Parent=MS.gene055991 |
| chr7.1 | exon | 14263037 | 14263096 | 14263037 | ID=MS.gene055991.t1.exon1;Parent=MS.gene055991.t1 |
| chr7.1 | CDS | 14263037 | 14263096 | 14263037 | ID=cds.MS.gene055991.t1;Parent=MS.gene055991.t1 |
| chr7.1 | exon | 14263314 | 14263707 | 14263314 | ID=MS.gene055991.t1.exon2;Parent=MS.gene055991.t1 |
| chr7.1 | CDS | 14263314 | 14263707 | 14263314 | ID=cds.MS.gene055991.t1;Parent=MS.gene055991.t1 |
| chr7.1 | exon | 14264055 | 14264668 | 14264055 | ID=MS.gene055991.t1.exon3;Parent=MS.gene055991.t1 |
| chr7.1 | CDS | 14264055 | 14264668 | 14264055 | ID=cds.MS.gene055991.t1;Parent=MS.gene055991.t1 |
| chr7.1 | exon | 14264842 | 14264907 | 14264842 | ID=MS.gene055991.t1.exon4;Parent=MS.gene055991.t1 |
| chr7.1 | CDS | 14264842 | 14264907 | 14264842 | ID=cds.MS.gene055991.t1;Parent=MS.gene055991.t1 |
| Gene Sequence |
| Protein sequence |
