Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31154.t1 | XP_013465392.1 | 100 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 1.40E-216 | 761.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31154.t1 | O81221 | 97.3 | 377 | 10 | 0 | 1 | 377 | 1 | 377 | 5.9e-216 | 751.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31154.t1 | A0A072VCE0 | 100.0 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 1.0e-216 | 761.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052120 | MS.gene31154 | 0.970152 | 3.48E-131 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31154.t1 | MTR_2g096840 | 100.000 | 377 | 0 | 0 | 1 | 377 | 1 | 377 | 0.0 | 784 |
| MS.gene31154.t1 | MTR_0015s0130 | 98.674 | 377 | 5 | 0 | 1 | 377 | 1 | 377 | 0.0 | 776 |
| MS.gene31154.t1 | MTR_7g026230 | 97.082 | 377 | 11 | 0 | 1 | 377 | 1 | 377 | 0.0 | 768 |
| MS.gene31154.t1 | MTR_6g004000 | 97.082 | 377 | 11 | 0 | 1 | 377 | 1 | 377 | 0.0 | 768 |
| MS.gene31154.t1 | MTR_7g093260 | 96.021 | 377 | 15 | 0 | 1 | 377 | 1 | 377 | 0.0 | 765 |
| MS.gene31154.t1 | MTR_3g095530 | 94.960 | 377 | 19 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene31154.t1 | MTR_2g008050 | 93.634 | 377 | 24 | 0 | 1 | 377 | 1 | 377 | 0.0 | 752 |
| MS.gene31154.t1 | MTR_7g027185 | 42.480 | 379 | 204 | 3 | 10 | 374 | 7 | 385 | 1.41e-118 | 349 |
| MS.gene31154.t1 | MTR_7g027185 | 43.662 | 355 | 187 | 2 | 10 | 351 | 7 | 361 | 7.33e-116 | 341 |
| MS.gene31154.t1 | MTR_7g027185 | 46.580 | 307 | 162 | 1 | 10 | 314 | 7 | 313 | 2.75e-108 | 320 |
| MS.gene31154.t1 | MTR_5g066400 | 37.360 | 447 | 206 | 11 | 1 | 376 | 1 | 444 | 4.42e-93 | 286 |
| MS.gene31154.t1 | MTR_8g464900 | 36.574 | 432 | 201 | 10 | 1 | 362 | 1 | 429 | 1.71e-86 | 268 |
| MS.gene31154.t1 | MTR_5g066400 | 34.753 | 446 | 191 | 11 | 1 | 376 | 1 | 416 | 2.52e-80 | 252 |
| MS.gene31154.t1 | MTR_8g089630 | 34.550 | 411 | 219 | 8 | 10 | 372 | 10 | 418 | 5.49e-72 | 231 |
| MS.gene31154.t1 | MTR_5g066400 | 34.250 | 400 | 192 | 10 | 1 | 332 | 1 | 397 | 4.11e-69 | 223 |
| MS.gene31154.t1 | MTR_3g030550 | 37.105 | 380 | 213 | 9 | 7 | 377 | 1 | 363 | 1.07e-67 | 218 |
| MS.gene31154.t1 | MTR_2g445210 | 28.132 | 423 | 238 | 7 | 8 | 366 | 6 | 426 | 3.20e-55 | 188 |
| MS.gene31154.t1 | MTR_8g007440 | 35.227 | 264 | 144 | 7 | 77 | 319 | 9 | 266 | 3.45e-46 | 159 |
| MS.gene31154.t1 | MTR_2g445210 | 26.613 | 372 | 207 | 7 | 8 | 315 | 6 | 375 | 3.38e-41 | 149 |
| MS.gene31154.t1 | MTR_3g010170 | 30.303 | 297 | 183 | 9 | 91 | 371 | 179 | 467 | 2.14e-40 | 149 |
| MS.gene31154.t1 | MTR_8g070520 | 31.058 | 293 | 175 | 10 | 91 | 366 | 187 | 469 | 7.96e-36 | 136 |
| MS.gene31154.t1 | MTR_8g070520 | 30.576 | 278 | 167 | 9 | 91 | 352 | 187 | 454 | 1.62e-32 | 127 |
| MS.gene31154.t1 | MTR_3g067945 | 28.968 | 252 | 160 | 7 | 9 | 245 | 21 | 268 | 9.70e-22 | 97.4 |
| MS.gene31154.t1 | MTR_3g067945 | 33.607 | 122 | 78 | 2 | 250 | 368 | 586 | 707 | 4.69e-16 | 80.1 |
| MS.gene31154.t1 | MTR_8g008540 | 70.270 | 37 | 11 | 0 | 340 | 376 | 41 | 77 | 4.15e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31154.t1 | AT3G12110 | 96.552 | 377 | 13 | 0 | 1 | 377 | 1 | 377 | 0.0 | 765 |
| MS.gene31154.t1 | AT3G53750 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene31154.t1 | AT3G53750 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene31154.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene31154.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene31154.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene31154.t1 | AT2G37620 | 95.756 | 377 | 16 | 0 | 1 | 377 | 1 | 377 | 0.0 | 763 |
| MS.gene31154.t1 | AT5G59370 | 96.817 | 377 | 12 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MS.gene31154.t1 | AT5G59370 | 96.817 | 377 | 12 | 0 | 1 | 377 | 1 | 377 | 0.0 | 762 |
| MS.gene31154.t1 | AT3G46520 | 96.552 | 377 | 13 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene31154.t1 | AT3G46520 | 96.552 | 377 | 13 | 0 | 1 | 377 | 1 | 377 | 0.0 | 761 |
| MS.gene31154.t1 | AT5G09810 | 94.960 | 377 | 19 | 0 | 1 | 377 | 1 | 377 | 0.0 | 759 |
| MS.gene31154.t1 | AT1G49240 | 92.573 | 377 | 28 | 0 | 1 | 377 | 1 | 377 | 0.0 | 745 |
| MS.gene31154.t1 | AT3G18780 | 92.308 | 377 | 29 | 0 | 1 | 377 | 1 | 377 | 0.0 | 744 |
| MS.gene31154.t1 | AT3G18780 | 92.308 | 377 | 29 | 0 | 1 | 377 | 1 | 377 | 0.0 | 744 |
| MS.gene31154.t1 | AT3G18780 | 92.201 | 359 | 28 | 0 | 1 | 359 | 1 | 359 | 0.0 | 703 |
| MS.gene31154.t1 | AT2G42100 | 83.967 | 368 | 59 | 0 | 10 | 377 | 11 | 378 | 0.0 | 673 |
| MS.gene31154.t1 | AT2G42090 | 70.000 | 370 | 106 | 1 | 7 | 376 | 1 | 365 | 0.0 | 564 |
| MS.gene31154.t1 | AT2G42170 | 77.108 | 332 | 73 | 1 | 46 | 377 | 1 | 329 | 0.0 | 556 |
| MS.gene31154.t1 | AT2G42170 | 77.108 | 332 | 73 | 1 | 46 | 377 | 1 | 329 | 0.0 | 556 |
| MS.gene31154.t1 | AT2G42170 | 76.923 | 312 | 69 | 1 | 46 | 357 | 1 | 309 | 0.0 | 518 |
| MS.gene31154.t1 | AT2G42170 | 76.923 | 312 | 69 | 1 | 46 | 357 | 1 | 309 | 0.0 | 518 |
| MS.gene31154.t1 | AT2G42170 | 76.991 | 226 | 49 | 1 | 152 | 377 | 9 | 231 | 2.18e-130 | 373 |
| MS.gene31154.t1 | AT3G27000 | 43.536 | 379 | 200 | 4 | 10 | 374 | 7 | 385 | 4.89e-121 | 355 |
| MS.gene31154.t1 | AT1G18450 | 37.472 | 443 | 207 | 9 | 1 | 376 | 1 | 440 | 1.50e-95 | 292 |
| MS.gene31154.t1 | AT1G13180 | 34.223 | 412 | 219 | 9 | 10 | 372 | 9 | 417 | 2.76e-72 | 232 |
| MS.gene31154.t1 | AT3G60830 | 37.467 | 379 | 213 | 9 | 7 | 377 | 1 | 363 | 5.82e-67 | 216 |
| MS.gene31154.t1 | AT3G33520 | 30.166 | 421 | 236 | 7 | 10 | 377 | 5 | 420 | 5.44e-58 | 194 |
| MS.gene31154.t1 | AT5G56180 | 31.338 | 284 | 170 | 9 | 91 | 358 | 50 | 324 | 1.95e-35 | 132 |
| MS.gene31154.t1 | AT5G56180 | 31.338 | 284 | 170 | 9 | 91 | 358 | 182 | 456 | 1.19e-34 | 133 |
| MS.gene31154.t1 | AT3G12380 | 29.183 | 257 | 159 | 6 | 9 | 245 | 21 | 274 | 1.21e-24 | 106 |
| MS.gene31154.t1 | AT3G12380 | 32.738 | 168 | 106 | 4 | 207 | 368 | 550 | 716 | 3.86e-17 | 83.6 |
| MS.gene31154.t1 | AT3G12380 | 29.020 | 255 | 156 | 6 | 9 | 245 | 21 | 268 | 4.83e-24 | 104 |
| MS.gene31154.t1 | AT3G12380 | 32.738 | 168 | 106 | 4 | 207 | 368 | 544 | 710 | 3.74e-17 | 83.6 |
| MS.gene31154.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.08e-23 | 95.1 |
| MS.gene31154.t1 | AT1G73910 | 35.862 | 145 | 74 | 3 | 1 | 129 | 1 | 142 | 2.08e-23 | 95.1 |
| MS.gene31154.t1 | AT5G56180 | 32.178 | 202 | 114 | 8 | 91 | 281 | 182 | 371 | 2.08e-19 | 89.4 |
| MS.gene31154.t1 | AT1G73910 | 29.268 | 123 | 71 | 2 | 1 | 107 | 1 | 123 | 3.00e-12 | 64.7 |
Find 100 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGATCTTCATGCTGCTT+GGG | 0.250595 | 2.4:+6585152 | None:intergenic |
| AGCAAAACCGGGTGCTCCTC+TGG | 0.272892 | 2.4:+6586100 | None:intergenic |
| CAACCACTGGTCTGTGATAA+TGG | 0.278942 | 2.4:-6586710 | MS.gene31154:CDS |
| AACATTTAGGCTGGATTTGC+TGG | 0.289528 | 2.4:-6586346 | MS.gene31154:intron |
| TGCTATGTATGTTGCTATTC+AGG | 0.305017 | 2.4:-6586002 | MS.gene31154:CDS |
| GGGATGACATGGAGAAAATT+TGG | 0.308086 | 2.4:-6586156 | MS.gene31154:CDS |
| AGGGCATATCCTTCATAAAT+AGG | 0.317673 | 2.4:+6585625 | None:intergenic |
| CACACGGAGTTCATTGTAAA+AGG | 0.330285 | 2.4:+6586126 | None:intergenic |
| ACCTTGATCTTCATGCTGCT+TGG | 0.336588 | 2.4:+6585151 | None:intergenic |
| AGCATGGTATTGTGAGTAAC+TGG | 0.340011 | 2.4:-6586177 | MS.gene31154:CDS |
| GGTGCTTCAGTGAGCAAAAC+CGG | 0.396077 | 2.4:+6586088 | None:intergenic |
| CTCACAATACCATGCTCAAT+TGG | 0.397001 | 2.4:+6586184 | None:intergenic |
| CAAGCTCTTGCTCAAAATCA+AGG | 0.399460 | 2.4:+6585455 | None:intergenic |
| CTCATTCTGTCAGCAATGCC+AGG | 0.400398 | 2.4:+6585196 | None:intergenic |
| ATCCTTCGTCTTGATTTAGC+AGG | 0.412905 | 2.4:-6585592 | MS.gene31154:CDS |
| GATGGCCAGATCATCACTAT+TGG | 0.420978 | 2.4:-6585385 | MS.gene31154:CDS |
| CCGAGAGAAAATACAGTGTC+TGG | 0.425088 | 2.4:-6585117 | MS.gene31154:CDS |
| GCAAAACCGGGTGCTCCTCT+GGG | 0.429592 | 2.4:+6586101 | None:intergenic |
| TACAATTTCACGCTCGGCTG+TGG | 0.436320 | 2.4:+6585510 | None:intergenic |
| TATGGAAATATTGTCCTTAG+TGG | 0.444109 | 2.4:-6585235 | MS.gene31154:CDS |
| CACTGGTGTGATGGTTGGCA+TGG | 0.449957 | 2.4:-6586272 | MS.gene31154:CDS |
| GATATCAGAAAGGACTTGTA+TGG | 0.450042 | 2.4:-6585253 | MS.gene31154:CDS |
| AGTGCTAAGAGAAGCTAAGA+TGG | 0.454030 | 2.4:+6585084 | None:intergenic |
| CTTGATCTTCATGCTGCTTG+GGG | 0.455780 | 2.4:+6585153 | None:intergenic |
| TATGCTAGTGGTCGAACAAC+CGG | 0.457727 | 2.4:-6585965 | MS.gene31154:intron |
| CAGCTCCAATAGTGATGATC+TGG | 0.458059 | 2.4:+6585380 | None:intergenic |
| ATCTCTTACAATTTCACGCT+CGG | 0.471407 | 2.4:+6585504 | None:intergenic |
| GCTGTGTTTCCTAGCATTGT+TGG | 0.477335 | 2.4:-6586307 | MS.gene31154:CDS |
| TAATGGAACAGGAATGGTTA+AGG | 0.478719 | 2.4:-6586693 | MS.gene31154:intron |
| CTGGTCTGTGATAATGGAAC+AGG | 0.481376 | 2.4:-6586704 | MS.gene31154:CDS |
| AAATACAGTGTCTGGATTGG+TGG | 0.485676 | 2.4:-6585109 | MS.gene31154:CDS |
| CCAGCCTTCCATGGTTGGAA+TGG | 0.489084 | 2.4:-6585329 | MS.gene31154:CDS |
| ATAGTGATGATCTGGCCATC+AGG | 0.494142 | 2.4:+6585388 | None:intergenic |
| AAGGCCGAGTACGATGAATC+TGG | 0.498285 | 2.4:-6584916 | MS.gene31154:CDS |
| AAGAATAACCACGCTCGGTA+AGG | 0.505013 | 2.4:+6585539 | None:intergenic |
| ATGAAAATCCTTACCGAGCG+TGG | 0.509930 | 2.4:-6585547 | MS.gene31154:CDS |
| AGAAAATACAGTGTCTGGAT+TGG | 0.510090 | 2.4:-6585112 | MS.gene31154:CDS |
| CCATTCCAACCATGGAAGGC+TGG | 0.515654 | 2.4:+6585329 | None:intergenic |
| TAGAGTCAATATACCACGCT+TGG | 0.516667 | 2.4:+6586213 | None:intergenic |
| CACACTGTGCCTATTTATGA+AGG | 0.522782 | 2.4:-6585634 | MS.gene31154:CDS |
| ATGCTAGGAAACACAGCCCT+TGG | 0.526579 | 2.4:+6586313 | None:intergenic |
| TGCTGGAGATGATGCTCCAA+GGG | 0.531721 | 2.4:-6586329 | MS.gene31154:CDS |
| GGTGGTACTACTATGTTCCC+TGG | 0.534257 | 2.4:-6585214 | MS.gene31154:CDS |
| GCAGGTATTGTACTGGACTC+TGG | 0.536986 | 2.4:-6585670 | MS.gene31154:intron |
| CGATTAGCCTTTGGATTGAG+CGG | 0.541575 | 2.4:+6586067 | None:intergenic |
| GACCAGTGGTTGAATATCCT+CGG | 0.542292 | 2.4:+6586721 | None:intergenic |
| CGCCGAGGATATTCAACCAC+TGG | 0.542623 | 2.4:-6586723 | MS.gene31154:CDS |
| CCCAAGCAGCATGAAGATCA+AGG | 0.543751 | 2.4:-6585152 | MS.gene31154:CDS |
| AATATGTGCAGGTATTGTAC+TGG | 0.544002 | 2.4:-6585677 | MS.gene31154:intron |
| TCCTTCGTCTTGATTTAGCA+GGG | 0.546953 | 2.4:-6585591 | MS.gene31154:CDS |
| GAAGGCTGGAAAAGAACCTC+AGG | 0.547727 | 2.4:+6585343 | None:intergenic |
| TGAAATTGTAAGAGATGTGA+AGG | 0.550481 | 2.4:-6585497 | MS.gene31154:CDS |
| CGTGGTCGACCAACAATGCT+AGG | 0.552232 | 2.4:+6586298 | None:intergenic |
| ACATAGTAGTACCACCACTA+AGG | 0.559599 | 2.4:+6585221 | None:intergenic |
| TTGCTGGAGATGATGCTCCA+AGG | 0.560432 | 2.4:-6586330 | MS.gene31154:CDS |
| GACGAAGGATAGCATGTGGA+AGG | 0.560879 | 2.4:+6585605 | None:intergenic |
| AAGCTCTTGCTCAAAATCAA+GGG | 0.566750 | 2.4:+6585456 | None:intergenic |
| ACCACGCCACACTGGTGTGA+TGG | 0.574183 | 2.4:-6586281 | MS.gene31154:CDS |
| CTAAAATATCCAATTGAGCA+TGG | 0.574515 | 2.4:-6586193 | MS.gene31154:CDS |
| TGTGGCCCCAGAGGAGCACC+CGG | 0.576326 | 2.4:-6586107 | MS.gene31154:CDS |
| TGAATAGCAACATACATAGC+AGG | 0.576757 | 2.4:+6586004 | None:intergenic |
| CGCCACACTGGTGTGATGGT+TGG | 0.577341 | 2.4:-6586277 | MS.gene31154:CDS |
| GTGCTTCAGTGAGCAAAACC+GGG | 0.578129 | 2.4:+6586089 | None:intergenic |
| GCTTCCATTCCAACCATGGA+AGG | 0.580286 | 2.4:+6585325 | None:intergenic |
| GGTGCTCCTCTGGGGCCACA+CGG | 0.580962 | 2.4:+6586110 | None:intergenic |
| AACAGCTTCCATTCCAACCA+TGG | 0.591828 | 2.4:+6585321 | None:intergenic |
| GAGCAAGGAAATTACCGCGT+TGG | 0.592403 | 2.4:-6585176 | MS.gene31154:CDS |
| GGAAATATTGTCCTTAGTGG+TGG | 0.594437 | 2.4:-6585232 | MS.gene31154:CDS |
| AGTGAAAGAATAACCACGCT+CGG | 0.601145 | 2.4:+6585534 | None:intergenic |
| AGGACCAGATTCATCGTACT+CGG | 0.601521 | 2.4:+6584912 | None:intergenic |
| TGTGAGTAACTGGGATGACA+TGG | 0.601686 | 2.4:-6586167 | MS.gene31154:CDS |
| TGAGCGTTTCCGTTGTCCTG+AGG | 0.602279 | 2.4:-6585359 | MS.gene31154:CDS |
| ATTGTACTGGACTCTGGAGA+TGG | 0.605459 | 2.4:-6585664 | MS.gene31154:CDS |
| GTTGGTCGACCACGCCACAC+TGG | 0.610992 | 2.4:-6586289 | MS.gene31154:CDS |
| ATGGTTGGAATGGAAGCTGT+TGG | 0.612805 | 2.4:-6585319 | MS.gene31154:CDS |
| TGAAGCACCGCTCAATCCAA+AGG | 0.625231 | 2.4:-6586074 | MS.gene31154:CDS |
| CATGCTGCTTGGGGCCAACG+CGG | 0.625414 | 2.4:+6585162 | None:intergenic |
| CATTGCTGACAGAATGAGCA+AGG | 0.625589 | 2.4:-6585191 | MS.gene31154:CDS |
| ACCATCACACCAGTGTGGCG+TGG | 0.628231 | 2.4:+6586280 | None:intergenic |
| CTGTGATAATGGAACAGGAA+TGG | 0.632340 | 2.4:-6586699 | MS.gene31154:CDS |
| ACTAGCATAGAGCGATAGAA+CGG | 0.636987 | 2.4:+6585979 | None:intergenic |
| CAAAACCGGGTGCTCCTCTG+GGG | 0.638105 | 2.4:+6586102 | None:intergenic |
| GTTCTATCGCTCTATGCTAG+TGG | 0.639915 | 2.4:-6585977 | MS.gene31154:CDS |
| TCAAGACGAAGGATAGCATG+TGG | 0.640472 | 2.4:+6585601 | None:intergenic |
| AAGAGTTATGAACTTCCTGA+TGG | 0.641331 | 2.4:-6585403 | MS.gene31154:CDS |
| TCATTCTGTCAGCAATGCCA+GGG | 0.641800 | 2.4:+6585197 | None:intergenic |
| ACTGGTGTGATGGTTGGCAT+GGG | 0.642331 | 2.4:-6586271 | MS.gene31154:CDS |
| TGAACTCCGTGTGGCCCCAG+AGG | 0.648993 | 2.4:-6586116 | MS.gene31154:CDS |
| AAAGACAATGGCAGACGCCG+AGG | 0.653794 | 2.4:-6586738 | None:intergenic |
| GGAAAAGAACCTCAGGACAA+CGG | 0.659232 | 2.4:+6585350 | None:intergenic |
| ACGAAGGATAGCATGTGGAA+GGG | 0.659324 | 2.4:+6585606 | None:intergenic |
| GATGAAGCTCAGTCCAAGCG+TGG | 0.663966 | 2.4:-6586226 | MS.gene31154:CDS |
| AATGCAGATGTGGATTGCAA+AGG | 0.664746 | 2.4:-6584935 | MS.gene31154:intron |
| GTGTGATGTTGATATCAGAA+AGG | 0.677167 | 2.4:-6585263 | MS.gene31154:CDS |
| GTTCCATTATCACAGACCAG+TGG | 0.693343 | 2.4:+6586707 | None:intergenic |
| TGCCAACCATCACACCAGTG+TGG | 0.706399 | 2.4:+6586275 | None:intergenic |
| GCATGGTATTGTGAGTAACT+GGG | 0.709006 | 2.4:-6586176 | MS.gene31154:CDS |
| GCCCTGCTAAATCAAGACGA+AGG | 0.714666 | 2.4:+6585590 | None:intergenic |
| CTTCATAAATAGGCACAGTG+TGG | 0.723850 | 2.4:+6585635 | None:intergenic |
| AAGCAGCATGAAGATCAAGG+TGG | 0.767710 | 2.4:-6585149 | MS.gene31154:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATTGTTCTATATCTA+TGG | - | chr2.4:6585878-6585897 | MS.gene31154:intron | 20.0% |
| !! | ATGTCAAAAAAAGAATTCTA+AGG | + | chr2.4:6586612-6586631 | None:intergenic | 20.0% |
| !!! | CATTCTAAGTTTTTTTATAC+TGG | - | chr2.4:6585725-6585744 | MS.gene31154:intron | 20.0% |
| !!! | TTGACATGTTTGATTTATTT+GGG | - | chr2.4:6586625-6586644 | MS.gene31154:intron | 20.0% |
| !!! | TTTGACATGTTTGATTTATT+TGG | - | chr2.4:6586624-6586643 | MS.gene31154:intron | 20.0% |
| ! | AGTGTATCACAAAACAATAT+TGG | + | chr2.4:6585075-6585094 | None:intergenic | 25.0% |
| !! | ACACATTAGTTTCTTAAATC+GGG | + | chr2.4:6585687-6585706 | None:intergenic | 25.0% |
| !! | GACACATTAGTTTCTTAAAT+CGG | + | chr2.4:6585688-6585707 | None:intergenic | 25.0% |
| !! | TATCTTTTCTGTTTTCTCTT+CGG | - | chr2.4:6585004-6585023 | MS.gene31154:intron | 25.0% |
| !!! | TATTTTTGAAAATGCAGATG+TGG | - | chr2.4:6586671-6586690 | MS.gene31154:intron | 25.0% |
| !!! | TCAGTTTGTTAATGGATATA+TGG | - | chr2.4:6585777-6585796 | MS.gene31154:intron | 25.0% |
| AAAAATAAGAATGCGTTGCA+AGG | - | chr2.4:6585753-6585772 | MS.gene31154:intron | 30.0% | |
| AACTCTATATGAATATGTGC+AGG | - | chr2.4:6585928-6585947 | MS.gene31154:intron | 30.0% | |
| AAGGGGAAAATAGACAAAAA+TGG | - | chr2.4:6585848-6585867 | MS.gene31154:intron | 30.0% | |
| AGTACTAAATGTTGTTGCTA+AGG | - | chr2.4:6585099-6585118 | MS.gene31154:CDS | 30.0% | |
| AGTTAAGTTCATGGGAAAAA+GGG | - | chr2.4:6585830-6585849 | MS.gene31154:intron | 30.0% | |
| ATGAAGTCTAGTTAAGTTCA+TGG | - | chr2.4:6585821-6585840 | MS.gene31154:intron | 30.0% | |
| CTAAAATATCCAATTGAGCA+TGG | - | chr2.4:6585423-6585442 | MS.gene31154:CDS | 30.0% | |
| TAGTTAAGTTCATGGGAAAA+AGG | - | chr2.4:6585829-6585848 | MS.gene31154:intron | 30.0% | |
| TATGGAAATATTGTCCTTAG+TGG | - | chr2.4:6586381-6586400 | MS.gene31154:intron | 30.0% | |
| TCACAGCAAATTAGTAGAAA+GGG | + | chr2.4:6585229-6585248 | None:intergenic | 30.0% | |
| TGAAATTGTAAGAGATGTGA+AGG | - | chr2.4:6586119-6586138 | MS.gene31154:CDS | 30.0% | |
| TGAAGTCTAGTTAAGTTCAT+GGG | - | chr2.4:6585822-6585841 | MS.gene31154:intron | 30.0% | |
| TTCACAGCAAATTAGTAGAA+AGG | + | chr2.4:6585230-6585249 | None:intergenic | 30.0% | |
| ! | CATTGTGATTTGCAACATTT+AGG | - | chr2.4:6585257-6585276 | MS.gene31154:CDS | 30.0% |
| ! | CATTTTCTGTGTACAATTGA+AGG | + | chr2.4:6586727-6586746 | None:intergenic | 30.0% |
| AAGAGTTATGAACTTCCTGA+TGG | - | chr2.4:6586213-6586232 | MS.gene31154:CDS | 35.0% | |
| AAGCTCTTGCTCAAAATCAA+GGG | + | chr2.4:6586163-6586182 | None:intergenic | 35.0% | |
| AGAAAATACAGTGTCTGGAT+TGG | - | chr2.4:6586504-6586523 | MS.gene31154:intron | 35.0% | |
| AGGGCATATCCTTCATAAAT+AGG | + | chr2.4:6585994-6586013 | None:intergenic | 35.0% | |
| ATCTCTTACAATTTCACGCT+CGG | + | chr2.4:6586115-6586134 | None:intergenic | 35.0% | |
| GATATCAGAAAGGACTTGTA+TGG | - | chr2.4:6586363-6586382 | MS.gene31154:intron | 35.0% | |
| GGACAAAAAGATGCATATGT+TGG | - | chr2.4:6585366-6585385 | MS.gene31154:CDS | 35.0% | |
| GTGTGATGTTGATATCAGAA+AGG | - | chr2.4:6586353-6586372 | MS.gene31154:intron | 35.0% | |
| GTTAAGTTCATGGGAAAAAG+GGG | - | chr2.4:6585831-6585850 | MS.gene31154:intron | 35.0% | |
| TAAATCGGGAAATGACTAAC+CGG | + | chr2.4:6585673-6585692 | None:intergenic | 35.0% | |
| TAATGGAACAGGAATGGTTA+AGG | - | chr2.4:6584923-6584942 | MS.gene31154:CDS | 35.0% | |
| TGAATAGCAACATACATAGC+AGG | + | chr2.4:6585615-6585634 | None:intergenic | 35.0% | |
| ! | AATATGTGCAGGTATTGTAC+TGG | - | chr2.4:6585939-6585958 | MS.gene31154:intron | 35.0% |
| ! | TGCTATGTATGTTGCTATTC+AGG | - | chr2.4:6585614-6585633 | MS.gene31154:CDS | 35.0% |
| ! | TTCTCTTAGCACTTTTCAAC+AGG | - | chr2.4:6586542-6586561 | MS.gene31154:intron | 35.0% |
| ! | TTTTTCCTGAAGAAGCGATT+TGG | - | chr2.4:6584976-6584995 | MS.gene31154:intron | 35.0% |
| !! | TGCAAGGTTCAGTTTGTTAA+TGG | - | chr2.4:6585769-6585788 | MS.gene31154:intron | 35.0% |
| !!! | ATTTTCTCACGATTAGCCTT+TGG | + | chr2.4:6585561-6585580 | None:intergenic | 35.0% |
| AACATTTAGGCTGGATTTGC+TGG | - | chr2.4:6585270-6585289 | MS.gene31154:CDS | 40.0% | |
| AAGGCATGCATAAATACACG+CGG | + | chr2.4:6586593-6586612 | None:intergenic | 40.0% | |
| ACATAGTAGTACCACCACTA+AGG | + | chr2.4:6586398-6586417 | None:intergenic | 40.0% | |
| ACTAGCATAGAGCGATAGAA+CGG | + | chr2.4:6585640-6585659 | None:intergenic | 40.0% | |
| AGCATGGTATTGTGAGTAAC+TGG | - | chr2.4:6585439-6585458 | MS.gene31154:CDS | 40.0% | |
| AGTGAAAGAATAACCACGCT+CGG | + | chr2.4:6586085-6586104 | None:intergenic | 40.0% | |
| AGTGCTAAGAGAAGCTAAGA+TGG | + | chr2.4:6586535-6586554 | None:intergenic | 40.0% | |
| ATCCTTCGTCTTGATTTAGC+AGG | - | chr2.4:6586024-6586043 | MS.gene31154:CDS | 40.0% | |
| CAAGCTCTTGCTCAAAATCA+AGG | + | chr2.4:6586164-6586183 | None:intergenic | 40.0% | |
| CACACGGAGTTCATTGTAAA+AGG | + | chr2.4:6585493-6585512 | None:intergenic | 40.0% | |
| CACACTGTGCCTATTTATGA+AGG | - | chr2.4:6585982-6586001 | MS.gene31154:CDS | 40.0% | |
| CTCACAATACCATGCTCAAT+TGG | + | chr2.4:6585435-6585454 | None:intergenic | 40.0% | |
| CTTCATAAATAGGCACAGTG+TGG | + | chr2.4:6585984-6586003 | None:intergenic | 40.0% | |
| GCATGGTATTGTGAGTAACT+GGG | - | chr2.4:6585440-6585459 | MS.gene31154:CDS | 40.0% | |
| GGAAATATTGTCCTTAGTGG+TGG | - | chr2.4:6586384-6586403 | MS.gene31154:intron | 40.0% | |
| GGGATGACATGGAGAAAATT+TGG | - | chr2.4:6585460-6585479 | MS.gene31154:CDS | 40.0% | |
| TAGAGTCAATATACCACGCT+TGG | + | chr2.4:6585406-6585425 | None:intergenic | 40.0% | |
| TCCTTCGTCTTGATTTAGCA+GGG | - | chr2.4:6586025-6586044 | MS.gene31154:CDS | 40.0% | |
| ! | AAATACAGTGTCTGGATTGG+TGG | - | chr2.4:6586507-6586526 | MS.gene31154:intron | 40.0% |
| ! | AATGCAGATGTGGATTGCAA+AGG | - | chr2.4:6586681-6586700 | MS.gene31154:intron | 40.0% |
| ! | CAGACACTGTATTTTCTCTC+GGG | + | chr2.4:6586501-6586520 | None:intergenic | 40.0% |
| ! | CCAGACACTGTATTTTCTCT+CGG | + | chr2.4:6586502-6586521 | None:intergenic | 40.0% |
| ! | CTCGGTAAGGATTTTCATCA+AGG | + | chr2.4:6586067-6586086 | None:intergenic | 40.0% |
| ! | CTTTTACAATGAACTCCGTG+TGG | - | chr2.4:6585491-6585510 | MS.gene31154:CDS | 40.0% |
| ! | GTGATTTGCAACATTTAGGC+TGG | - | chr2.4:6585261-6585280 | MS.gene31154:CDS | 40.0% |
| ! | TCTTTTCTACGATCTGCGAT+TGG | - | chr2.4:6585173-6585192 | MS.gene31154:CDS | 40.0% |
| !! | CTGTGATAATGGAACAGGAA+TGG | - | chr2.4:6584917-6584936 | MS.gene31154:CDS | 40.0% |
| !! | TTTTCATCAAGGCATCAGTG+AGG | + | chr2.4:6586056-6586075 | None:intergenic | 40.0% |
| AACAGCTTCCATTCCAACCA+TGG | + | chr2.4:6586298-6586317 | None:intergenic | 45.0% | |
| AAGAATAACCACGCTCGGTA+AGG | + | chr2.4:6586080-6586099 | None:intergenic | 45.0% | |
| AAGCAGCATGAAGATCAAGG+TGG | - | chr2.4:6586467-6586486 | MS.gene31154:intron | 45.0% | |
| ACCTTGATCTTCATGCTGCT+TGG | + | chr2.4:6586468-6586487 | None:intergenic | 45.0% | |
| ACGAAGGATAGCATGTGGAA+GGG | + | chr2.4:6586013-6586032 | None:intergenic | 45.0% | |
| AGGACCAGATTCATCGTACT+CGG | + | chr2.4:6586707-6586726 | None:intergenic | 45.0% | |
| ATAGTGATGATCTGGCCATC+AGG | + | chr2.4:6586231-6586250 | None:intergenic | 45.0% | |
| ATGAAAATCCTTACCGAGCG+TGG | - | chr2.4:6586069-6586088 | MS.gene31154:CDS | 45.0% | |
| ATGGTTGGAATGGAAGCTGT+TGG | - | chr2.4:6586297-6586316 | MS.gene31154:CDS | 45.0% | |
| CAGCTCCAATAGTGATGATC+TGG | + | chr2.4:6586239-6586258 | None:intergenic | 45.0% | |
| CATAGCCAAATCGCTTCTTC+AGG | + | chr2.4:6584984-6585003 | None:intergenic | 45.0% | |
| CATTGCTGACAGAATGAGCA+AGG | - | chr2.4:6586425-6586444 | MS.gene31154:intron | 45.0% | |
| CCGAGAGAAAATACAGTGTC+TGG | - | chr2.4:6586499-6586518 | MS.gene31154:intron | 45.0% | |
| CGCGGACAATGTGAGTATAT+CGG | + | chr2.4:6586575-6586594 | None:intergenic | 45.0% | |
| GACCAGTGGTTGAATATCCT+CGG | + | chr2.4:6584898-6584917 | None:intergenic | 45.0% | |
| GATGGCCAGATCATCACTAT+TGG | - | chr2.4:6586231-6586250 | MS.gene31154:CDS | 45.0% | |
| GCTGTGTTTCCTAGCATTGT+TGG | - | chr2.4:6585309-6585328 | MS.gene31154:CDS | 45.0% | |
| GGAAAAGAACCTCAGGACAA+CGG | + | chr2.4:6586269-6586288 | None:intergenic | 45.0% | |
| GTTCCATTATCACAGACCAG+TGG | + | chr2.4:6584912-6584931 | None:intergenic | 45.0% | |
| GTTCTATCGCTCTATGCTAG+TGG | - | chr2.4:6585639-6585658 | MS.gene31154:CDS | 45.0% | |
| TATGCTAGTGGTCGAACAAC+CGG | - | chr2.4:6585651-6585670 | MS.gene31154:CDS | 45.0% | |
| TCAAGACGAAGGATAGCATG+TGG | + | chr2.4:6586018-6586037 | None:intergenic | 45.0% | |
| TCATTCTGTCAGCAATGCCA+GGG | + | chr2.4:6586422-6586441 | None:intergenic | 45.0% | |
| TGTGAGTAACTGGGATGACA+TGG | - | chr2.4:6585449-6585468 | MS.gene31154:CDS | 45.0% | |
| ! | ACACTGTATTTTCTCTCGGG+TGG | + | chr2.4:6586498-6586517 | None:intergenic | 45.0% |
| ! | ATTGTACTGGACTCTGGAGA+TGG | - | chr2.4:6585952-6585971 | MS.gene31154:intron | 45.0% |
| ! | CAACCACTGGTCTGTGATAA+TGG | - | chr2.4:6584906-6584925 | MS.gene31154:CDS | 45.0% |
| ! | CCTTGATCTTCATGCTGCTT+GGG | + | chr2.4:6586467-6586486 | None:intergenic | 45.0% |
| ! | CTTGATCTTCATGCTGCTTG+GGG | + | chr2.4:6586466-6586485 | None:intergenic | 45.0% |
| !! | CGATTAGCCTTTGGATTGAG+CGG | + | chr2.4:6585552-6585571 | None:intergenic | 45.0% |
| !! | CTGGTCTGTGATAATGGAAC+AGG | - | chr2.4:6584912-6584931 | MS.gene31154:CDS | 45.0% |
| AAGGCCGAGTACGATGAATC+TGG | - | chr2.4:6586700-6586719 | MS.gene31154:CDS | 50.0% | |
| ACTGGTGTGATGGTTGGCAT+GGG | - | chr2.4:6585345-6585364 | MS.gene31154:CDS | 50.0% | |
| ATGCTAGGAAACACAGCCCT+TGG | + | chr2.4:6585306-6585325 | None:intergenic | 50.0% | |
| CCCAAGCAGCATGAAGATCA+AGG | - | chr2.4:6586464-6586483 | MS.gene31154:intron | 50.0% | |
| CTCATTCTGTCAGCAATGCC+AGG | + | chr2.4:6586423-6586442 | None:intergenic | 50.0% | |
| GAAGGCTGGAAAAGAACCTC+AGG | + | chr2.4:6586276-6586295 | None:intergenic | 50.0% | |
| GACGAAGGATAGCATGTGGA+AGG | + | chr2.4:6586014-6586033 | None:intergenic | 50.0% | |
| GAGCAAGGAAATTACCGCGT+TGG | - | chr2.4:6586440-6586459 | MS.gene31154:intron | 50.0% | |
| GCCCTGCTAAATCAAGACGA+AGG | + | chr2.4:6586029-6586048 | None:intergenic | 50.0% | |
| GCTTCCATTCCAACCATGGA+AGG | + | chr2.4:6586294-6586313 | None:intergenic | 50.0% | |
| GGTGCTTCAGTGAGCAAAAC+CGG | + | chr2.4:6585531-6585550 | None:intergenic | 50.0% | |
| GGTGGTACTACTATGTTCCC+TGG | - | chr2.4:6586402-6586421 | MS.gene31154:intron | 50.0% | |
| GTGCTTCAGTGAGCAAAACC+GGG | + | chr2.4:6585530-6585549 | None:intergenic | 50.0% | |
| TACAATTTCACGCTCGGCTG+TGG | + | chr2.4:6586109-6586128 | None:intergenic | 50.0% | |
| TGCTGGAGATGATGCTCCAA+GGG | - | chr2.4:6585287-6585306 | MS.gene31154:CDS | 50.0% | |
| TTGCTGGAGATGATGCTCCA+AGG | - | chr2.4:6585286-6585305 | MS.gene31154:CDS | 50.0% | |
| ! | CTTTTCCAGCCTTCCATGGT+TGG | - | chr2.4:6586282-6586301 | MS.gene31154:CDS | 50.0% |
| ! | GCAGGTATTGTACTGGACTC+TGG | - | chr2.4:6585946-6585965 | MS.gene31154:intron | 50.0% |
| ! | GGTTCTTTTCCAGCCTTCCA+TGG | - | chr2.4:6586278-6586297 | MS.gene31154:CDS | 50.0% |
| !! | TGAAGCACCGCTCAATCCAA+AGG | - | chr2.4:6585542-6585561 | MS.gene31154:CDS | 50.0% |
| CACTGGTGTGATGGTTGGCA+TGG | - | chr2.4:6585344-6585363 | MS.gene31154:CDS | 55.0% | |
| CCAGCCTTCCATGGTTGGAA+TGG | - | chr2.4:6586287-6586306 | MS.gene31154:CDS | 55.0% | |
| CCATTCCAACCATGGAAGGC+TGG | + | chr2.4:6586290-6586309 | None:intergenic | 55.0% | |
| CGCCGAGGATATTCAACCAC+TGG | - | chr2.4:6584893-6584912 | MS.gene31154:CDS | 55.0% | |
| CGTGGTCGACCAACAATGCT+AGG | + | chr2.4:6585321-6585340 | None:intergenic | 55.0% | |
| GATGAAGCTCAGTCCAAGCG+TGG | - | chr2.4:6585390-6585409 | MS.gene31154:CDS | 55.0% | |
| TGAGCGTTTCCGTTGTCCTG+AGG | - | chr2.4:6586257-6586276 | MS.gene31154:CDS | 55.0% | |
| TGCCAACCATCACACCAGTG+TGG | + | chr2.4:6585344-6585363 | None:intergenic | 55.0% | |
| ACCACGCCACACTGGTGTGA+TGG | - | chr2.4:6585335-6585354 | MS.gene31154:CDS | 60.0% | |
| ACCATCACACCAGTGTGGCG+TGG | + | chr2.4:6585339-6585358 | None:intergenic | 60.0% | |
| AGCAAAACCGGGTGCTCCTC+TGG | + | chr2.4:6585519-6585538 | None:intergenic | 60.0% | |
| CAAAACCGGGTGCTCCTCTG+GGG | + | chr2.4:6585517-6585536 | None:intergenic | 60.0% | |
| CGCCACACTGGTGTGATGGT+TGG | - | chr2.4:6585339-6585358 | MS.gene31154:CDS | 60.0% | |
| GCAAAACCGGGTGCTCCTCT+GGG | + | chr2.4:6585518-6585537 | None:intergenic | 60.0% | |
| GTTGGTCGACCACGCCACAC+TGG | - | chr2.4:6585327-6585346 | MS.gene31154:CDS | 65.0% | |
| TGAACTCCGTGTGGCCCCAG+AGG | - | chr2.4:6585500-6585519 | MS.gene31154:CDS | 65.0% | |
| ! | CATGCTGCTTGGGGCCAACG+CGG | + | chr2.4:6586457-6586476 | None:intergenic | 65.0% |
| !! | GGTGCTCCTCTGGGGCCACA+CGG | + | chr2.4:6585509-6585528 | None:intergenic | 70.0% |
| !! | TGTGGCCCCAGAGGAGCACC+CGG | - | chr2.4:6585509-6585528 | MS.gene31154:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 6584885 | 6586753 | 6584885 | ID=MS.gene31154 |
| chr2.4 | mRNA | 6584885 | 6586753 | 6584885 | ID=MS.gene31154.t1;Parent=MS.gene31154 |
| chr2.4 | exon | 6586694 | 6586753 | 6586694 | ID=MS.gene31154.t1.exon1;Parent=MS.gene31154.t1 |
| chr2.4 | CDS | 6586694 | 6586753 | 6586694 | ID=cds.MS.gene31154.t1;Parent=MS.gene31154.t1 |
| chr2.4 | exon | 6585966 | 6586359 | 6585966 | ID=MS.gene31154.t1.exon2;Parent=MS.gene31154.t1 |
| chr2.4 | CDS | 6585966 | 6586359 | 6585966 | ID=cds.MS.gene31154.t1;Parent=MS.gene31154.t1 |
| chr2.4 | exon | 6585075 | 6585688 | 6585075 | ID=MS.gene31154.t1.exon3;Parent=MS.gene31154.t1 |
| chr2.4 | CDS | 6585075 | 6585688 | 6585075 | ID=cds.MS.gene31154.t1;Parent=MS.gene31154.t1 |
| chr2.4 | exon | 6584885 | 6584950 | 6584885 | ID=MS.gene31154.t1.exon4;Parent=MS.gene31154.t1 |
| chr2.4 | CDS | 6584885 | 6584950 | 6584885 | ID=cds.MS.gene31154.t1;Parent=MS.gene31154.t1 |
| Gene Sequence |
| Protein sequence |
