AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020921

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020921.t1 MTR_5g097900 98.397 312 5 0 1 312 1 312 0.0 637
MS.gene020921.t1 MTR_5g097910 98.077 312 6 0 1 312 1 312 0.0 635
MS.gene020921.t1 MTR_5g097940 98.077 312 6 0 1 312 1 312 0.0 635
MS.gene020921.t1 MTR_3g083130 68.182 308 97 1 6 312 7 314 8.66e-162 453
MS.gene020921.t1 MTR_0374s0050 62.951 305 110 3 1 302 1 305 3.16e-137 391
MS.gene020921.t1 MTR_4g072320 52.174 299 133 4 1 297 1 291 1.07e-100 298
MS.gene020921.t1 MTR_4g072350 51.155 303 136 5 20 311 17 318 6.14e-99 294
MS.gene020921.t1 MTR_4g072360 50.000 300 144 4 1 297 1 297 1.39e-98 293
MS.gene020921.t1 MTR_6g073110 49.835 303 146 4 1 300 1 300 3.89e-97 289
MS.gene020921.t1 MTR_8g070095 41.901 284 162 2 19 300 15 297 4.18e-73 228
MS.gene020921.t1 MTR_7g063580 43.382 272 142 3 49 311 83 351 4.68e-71 224
MS.gene020921.t1 MTR_4g036845 40.351 285 165 4 18 300 12 293 5.20e-70 220
MS.gene020921.t1 MTR_8g070115 42.361 288 155 4 19 302 15 295 1.92e-68 216
MS.gene020921.t1 MTR_4g021350 43.206 287 148 5 19 300 15 291 2.70e-65 208
MS.gene020921.t1 MTR_4g021410 42.295 305 148 6 19 312 16 303 5.85e-65 207
MS.gene020921.t1 MTR_4g021350 43.060 281 145 5 19 294 15 285 3.63e-63 201
MS.gene020921.t1 MTR_4g021410 43.223 273 135 4 48 312 6 266 1.59e-61 197
MS.gene020921.t1 MTR_4g021450 42.908 282 147 3 19 300 16 283 2.84e-61 197
MS.gene020921.t1 MTR_7g070500 37.500 280 167 5 20 298 23 295 8.22e-58 189
MS.gene020921.t1 MTR_7g070500 37.956 274 162 5 20 292 23 289 3.11e-57 187
MS.gene020921.t1 MTR_4g072060 38.436 307 174 6 15 310 6 308 1.13e-56 186
MS.gene020921.t1 MTR_4g021410 42.259 239 118 4 82 312 1 227 6.52e-48 160
MS.gene020921.t1 MTR_4g021450 43.379 219 112 2 82 300 1 207 3.31e-45 153
MS.gene020921.t1 MTR_4g021450 43.038 158 87 1 143 300 10 164 1.25e-38 135
MS.gene020921.t1 MTR_6g072650 43.537 147 47 5 176 311 13 134 2.32e-26 101
MS.gene020921.t1 MTR_6g072710 45.133 113 57 2 191 300 1 111 5.94e-25 97.1
MS.gene020921.t1 MTR_3g449790 26.625 323 159 12 15 285 42 338 6.56e-18 83.6
MS.gene020921.t1 MTR_3g449790 26.140 329 159 13 15 285 42 344 1.45e-16 79.7
MS.gene020921.t1 MTR_7g114990 26.736 288 147 13 41 289 52 314 1.17e-12 68.2
MS.gene020921.t1 MTR_7g021850 25.087 287 161 9 41 292 43 310 6.07e-12 65.9
MS.gene020921.t1 MTR_7g021680 24.742 291 166 9 36 292 38 309 5.94e-11 62.8
MS.gene020921.t1 MTR_7g021820 24.742 291 166 9 36 292 38 309 5.94e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020921.t1 AT1G59960 53.896 308 131 6 1 300 1 305 8.72e-113 330
MS.gene020921.t1 AT1G59950 55.903 288 117 5 20 300 15 299 3.18e-105 310
MS.gene020921.t1 AT2G37790 43.357 286 151 4 19 300 15 293 4.58e-76 236
MS.gene020921.t1 AT2G37770 43.357 286 151 4 19 300 15 293 2.42e-75 234
MS.gene020921.t1 AT5G62420 42.958 284 156 4 20 300 14 294 5.65e-73 228
MS.gene020921.t1 AT2G37760 42.612 291 140 5 19 299 15 288 4.11e-72 224
MS.gene020921.t1 AT2G37760 42.612 291 140 5 19 299 15 288 4.11e-72 224
MS.gene020921.t1 AT5G01670 41.812 287 158 6 16 300 20 299 4.25e-72 226
MS.gene020921.t1 AT2G37760 43.056 288 137 5 19 296 15 285 3.86e-71 222
MS.gene020921.t1 AT2G37760 42.561 289 139 5 19 297 15 286 2.97e-70 221
MS.gene020921.t1 AT2G37760 42.561 289 139 5 19 297 15 286 2.97e-70 221
MS.gene020921.t1 AT3G53880 41.343 283 161 3 19 300 15 293 1.32e-68 216
MS.gene020921.t1 AT3G53880 41.429 280 153 4 19 294 15 287 3.44e-66 210
MS.gene020921.t1 AT5G01670 38.264 311 156 7 19 300 23 326 4.58e-66 211
MS.gene020921.t1 AT2G21260 38.699 292 164 6 15 295 6 293 5.30e-65 207
MS.gene020921.t1 AT2G21250 37.459 307 177 6 15 310 6 308 1.02e-63 204
MS.gene020921.t1 AT2G37760 42.276 246 121 4 62 297 6 240 8.62e-58 187
MS.gene020921.t1 AT2G37760 42.276 246 121 4 62 297 6 240 8.62e-58 187
MS.gene020921.t1 AT2G37770 46.193 197 96 3 19 212 15 204 1.33e-52 174
MS.gene020921.t1 AT2G21250 41.429 210 112 5 15 217 6 211 1.02e-46 157
MS.gene020921.t1 AT2G21260 35.000 220 130 5 87 295 8 225 7.02e-40 140
MS.gene020921.t1 AT1G06690 27.397 292 144 10 35 282 85 352 6.36e-20 89.4
MS.gene020921.t1 AT5G53580 25.263 285 158 10 44 290 80 347 5.29e-16 78.2
MS.gene020921.t1 AT1G60680 25.982 331 178 15 15 300 14 322 3.98e-14 72.4
MS.gene020921.t1 AT1G60750 25.177 282 161 11 44 300 50 306 1.64e-11 64.7

Find 62 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAAGATACACAAATTT+TGG 0.147232 5.4:+77171210 None:intergenic
TTGCATTGCAAAACTCTCTA+AGG 0.274401 5.4:+77171426 None:intergenic
TGGACTTGTACTTGATTCAT+TGG 0.300645 5.4:-77171775 MS.gene020921:CDS
ATTGGAAATGTAAACTTTCC+AGG 0.312952 5.4:+77171737 None:intergenic
GTGTCTATAACCTTGTTTGA+TGG 0.333209 5.4:+77172219 None:intergenic
AGGCCTGGCTTGGTTGGTCC+AGG 0.344379 5.4:+77171128 None:intergenic
AAAATTTGTGTATCTTTGAT+TGG 0.361005 5.4:-77171208 MS.gene020921:CDS
CATTTGATGTGAAAGGTGTT+TGG 0.375654 5.4:-77171694 MS.gene020921:CDS
CTTGGACTCACTAAAGCTAT+TGG 0.375778 5.4:-77171647 MS.gene020921:CDS
CAAACACCTTTCACATCAAA+TGG 0.378027 5.4:+77171695 None:intergenic
AAGAGATCTGCCACATCAAT+TGG 0.378807 5.4:+77171719 None:intergenic
TCATTCTCCATAACCTCATT+TGG 0.392662 5.4:+77171350 None:intergenic
TTGCACAAATTTCTCTAAGA+TGG 0.398611 5.4:-77171289 MS.gene020921:CDS
TCTTTCCTTATCATAACTCT+TGG 0.404407 5.4:+77171238 None:intergenic
CAGAGGACCAAATGAGGTTA+TGG 0.408273 5.4:-77171357 MS.gene020921:CDS
ATTTGATGTGAAAGGTGTTT+GGG 0.418218 5.4:-77171693 MS.gene020921:CDS
TTGAAAGAGGCAATTGAACT+TGG 0.422526 5.4:-77172148 MS.gene020921:CDS
TGTTCCCAAGAGTTATGATA+AGG 0.428240 5.4:-77171243 MS.gene020921:CDS
TTTGATACTGCTGCTGCCTA+TGG 0.438326 5.4:-77172196 MS.gene020921:CDS
TCATTGAGGCCTGGCTTGGT+TGG 0.438358 5.4:+77171122 None:intergenic
CTTTCCTTATCATAACTCTT+GGG 0.442857 5.4:+77171239 None:intergenic
AAGCAAAATCGTTTGATCCC+TGG 0.455139 5.4:-77171146 MS.gene020921:CDS
GACTCTTCAACTAGACTACT+TGG 0.469433 5.4:-77171795 MS.gene020921:CDS
GAGGTCATTGAGGCCTGGCT+TGG 0.476217 5.4:+77171118 None:intergenic
ATCCCTGGACCAACCAAGCC+AGG 0.483424 5.4:-77171131 MS.gene020921:CDS
AAGTTTCAAGCTTTCCTCCA+TGG 0.483539 5.4:+77171667 None:intergenic
GGCCTGGCTTGGTTGGTCCA+GGG 0.485174 5.4:+77171129 None:intergenic
TGTTGGTAAGAACCTTGGTT+GGG 0.497536 5.4:+77172332 None:intergenic
ATGGAGGAAAGCTTGAAACT+TGG 0.498400 5.4:-77171665 MS.gene020921:CDS
TCTTGGTGAGGCTTTGAAAG+AGG 0.504118 5.4:-77172161 MS.gene020921:CDS
TCTTTCTTACATGTGAAGTC+AGG 0.515291 5.4:+77172265 None:intergenic
CTCTTACCATTTGATGTGAA+AGG 0.516557 5.4:-77171701 MS.gene020921:CDS
CTTCAATTGACTAGAAGTGT+TGG 0.534788 5.4:+77172315 None:intergenic
GAAGATGCCTGTGGTTGGAA+TGG 0.535658 5.4:-77172296 MS.gene020921:CDS
TATGGTTCAGAACAAGCTCT+TGG 0.542359 5.4:-77172178 MS.gene020921:CDS
TCATAGAGGTCATTGAGGCC+TGG 0.545875 5.4:+77171113 None:intergenic
AAGAGCTTGTTCTGAACCAT+AGG 0.546096 5.4:+77172180 None:intergenic
AGAAGTGTTGGTAAGAACCT+TGG 0.552345 5.4:+77172327 None:intergenic
AAGATGCCTGTGGTTGGAAT+GGG 0.555328 5.4:-77172295 MS.gene020921:CDS
TTGACATTTAGTCATCATAG+AGG 0.571928 5.4:+77171099 None:intergenic
GTGTTGGTAAGAACCTTGGT+TGG 0.585257 5.4:+77172331 None:intergenic
ATCATTGAAGCCATCAAACA+AGG 0.587661 5.4:-77172229 MS.gene020921:CDS
GAAAGGTGTTTGGGAATCCA+TGG 0.587744 5.4:-77171684 MS.gene020921:CDS
GCTGATCCCATTCCAACCAC+AGG 0.587926 5.4:+77172289 None:intergenic
CTAAGATGGCTATATGAACA+AGG 0.588672 5.4:-77171275 MS.gene020921:CDS
CTGCTTGCACCTTTCCTCAA+CGG 0.589452 5.4:+77171377 None:intergenic
AGGTGGAGATGAACCTTGCA+TGG 0.591438 5.4:-77171460 MS.gene020921:CDS
TGCAAGCAGAGGACCAAATG+AGG 0.592065 5.4:-77171363 MS.gene020921:CDS
TGAATTACTTGATTGACTGC+AGG 0.596137 5.4:+77171566 None:intergenic
AGTCATCATAGAGGTCATTG+AGG 0.605914 5.4:+77171108 None:intergenic
GTTTACATTTCCAATTGATG+TGG 0.608506 5.4:-77171729 MS.gene020921:CDS
CAATTGAAGATGCCTGTGGT+TGG 0.621459 5.4:-77172301 MS.gene020921:CDS
TGGCCACTTAGTTCTCAACC+TGG 0.621982 5.4:-77171755 MS.gene020921:CDS
AGAGCAGGAATAACAAGATG+AGG 0.624425 5.4:+77172067 None:intergenic
TTTCCAGGTTGAGAACTAAG+TGG 0.629025 5.4:+77171752 None:intergenic
AAAGAGATTGCAGATGCACA+TGG 0.633321 5.4:-77171320 MS.gene020921:CDS
TTCAGAACAAGCTCTTGGTG+AGG 0.637219 5.4:-77172173 MS.gene020921:CDS
TAGTCAATTGAAGATGCCTG+TGG 0.637967 5.4:-77172305 MS.gene020921:CDS
TTGAGGAAAGGTGCAAGCAG+AGG 0.639249 5.4:-77171374 MS.gene020921:CDS
TAGTGTTGAAATCCCAACCA+AGG 0.690652 5.4:-77172344 MS.gene020921:CDS
ATTGACTGCAGGAAGAACAG+TGG 0.718468 5.4:+77171577 None:intergenic
AGGTGTTTGGGAATCCATGG+AGG 0.719619 5.4:-77171681 MS.gene020921:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTTGTGTATCTTTGAT+TGG - chr5.4:77172247-77172266 MS.gene020921:CDS 20.0%
!!! AATCAAAGATACACAAATTT+TGG + chr5.4:77172248-77172267 None:intergenic 20.0%
! CAAGAAGTTAATCAAAACTT+AGG + chr5.4:77171924-77171943 None:intergenic 25.0%
! CAATGAACTCATATATTTAC+AGG - chr5.4:77171975-77171994 MS.gene020921:intron 25.0%
! GAGTATATTTAGATGACATT+TGG - chr5.4:77171456-77171475 MS.gene020921:CDS 25.0%
! TACACTTATTGAGATTAAAC+TGG - chr5.4:77171618-77171637 MS.gene020921:CDS 25.0%
!!! TTTTGTTACTTCCAAACTTT+GGG - chr5.4:77171351-77171370 MS.gene020921:CDS 25.0%
!!! TTTTTGTTACTTCCAAACTT+TGG - chr5.4:77171350-77171369 MS.gene020921:CDS 25.0%
ATTGGAAATGTAAACTTTCC+AGG + chr5.4:77171721-77171740 None:intergenic 30.0%
CTTTCCTTATCATAACTCTT+GGG + chr5.4:77172219-77172238 None:intergenic 30.0%
GTTTACATTTCCAATTGATG+TGG - chr5.4:77171726-77171745 MS.gene020921:CDS 30.0%
TCTTTCCTTATCATAACTCT+TGG + chr5.4:77172220-77172239 None:intergenic 30.0%
TGAACTCATATATTTACAGG+TGG - chr5.4:77171978-77171997 MS.gene020921:intron 30.0%
! ATTTTCAGTAACCCAAAGTT+TGG + chr5.4:77171365-77171384 None:intergenic 30.0%
! TTGCACAAATTTCTCTAAGA+TGG - chr5.4:77172166-77172185 MS.gene020921:CDS 30.0%
!! ATTTGATGTGAAAGGTGTTT+GGG - chr5.4:77171762-77171781 MS.gene020921:CDS 30.0%
ATCATTGAAGCCATCAAACA+AGG - chr5.4:77171226-77171245 MS.gene020921:CDS 35.0%
CAAACACCTTTCACATCAAA+TGG + chr5.4:77171763-77171782 None:intergenic 35.0%
CTAAGATGGCTATATGAACA+AGG - chr5.4:77172180-77172199 MS.gene020921:CDS 35.0%
CTCTTACCATTTGATGTGAA+AGG - chr5.4:77171754-77171773 MS.gene020921:CDS 35.0%
TCATTCTCCATAACCTCATT+TGG + chr5.4:77172108-77172127 None:intergenic 35.0%
TCTTTCTTACATGTGAAGTC+AGG + chr5.4:77171193-77171212 None:intergenic 35.0%
TGAATTACTTGATTGACTGC+AGG + chr5.4:77171892-77171911 None:intergenic 35.0%
TGGACTTGTACTTGATTCAT+TGG - chr5.4:77171680-77171699 MS.gene020921:CDS 35.0%
TGTTCCCAAGAGTTATGATA+AGG - chr5.4:77172212-77172231 MS.gene020921:CDS 35.0%
TTGAAAGAGGCAATTGAACT+TGG - chr5.4:77171307-77171326 MS.gene020921:CDS 35.0%
TTGCATTGCAAAACTCTCTA+AGG + chr5.4:77172032-77172051 None:intergenic 35.0%
! AGAGAGTTTTGCAATGCAAA+TGG - chr5.4:77172033-77172052 MS.gene020921:intron 35.0%
! CTTCAATTGACTAGAAGTGT+TGG + chr5.4:77171143-77171162 None:intergenic 35.0%
! GTGTCTATAACCTTGTTTGA+TGG + chr5.4:77171239-77171258 None:intergenic 35.0%
!! CATTTGATGTGAAAGGTGTT+TGG - chr5.4:77171761-77171780 MS.gene020921:CDS 35.0%
!! TTGAGAGATTTTTGAAGAGC+AGG + chr5.4:77171406-77171425 None:intergenic 35.0%
AAAGAGATTGCAGATGCACA+TGG - chr5.4:77172135-77172154 MS.gene020921:CDS 40.0%
AAGAGATCTGCCACATCAAT+TGG + chr5.4:77171739-77171758 None:intergenic 40.0%
AAGCAAAATCGTTTGATCCC+TGG - chr5.4:77172309-77172328 MS.gene020921:CDS 40.0%
AAGTTTCAAGCTTTCCTCCA+TGG + chr5.4:77171791-77171810 None:intergenic 40.0%
AGAGCAGGAATAACAAGATG+AGG + chr5.4:77171391-77171410 None:intergenic 40.0%
AGTCATCATAGAGGTCATTG+AGG + chr5.4:77172350-77172369 None:intergenic 40.0%
ATGGAGGAAAGCTTGAAACT+TGG - chr5.4:77171790-77171809 MS.gene020921:CDS 40.0%
CTTGGACTCACTAAAGCTAT+TGG - chr5.4:77171808-77171827 MS.gene020921:intron 40.0%
GACTCTTCAACTAGACTACT+TGG - chr5.4:77171660-77171679 MS.gene020921:CDS 40.0%
TAGTCAATTGAAGATGCCTG+TGG - chr5.4:77171150-77171169 MS.gene020921:CDS 40.0%
TATGGTTCAGAACAAGCTCT+TGG - chr5.4:77171277-77171296 MS.gene020921:CDS 40.0%
! AAGAGCTTGTTCTGAACCAT+AGG + chr5.4:77171278-77171297 None:intergenic 40.0%
! TAGTGTTGAAATCCCAACCA+AGG - chr5.4:77171111-77171130 MS.gene020921:CDS 40.0%
! TGTTGGTAAGAACCTTGGTT+GGG + chr5.4:77171126-77171145 None:intergenic 40.0%
! TTTCCAGGTTGAGAACTAAG+TGG + chr5.4:77171706-77171725 None:intergenic 40.0%
!! AGAAGTGTTGGTAAGAACCT+TGG + chr5.4:77171131-77171150 None:intergenic 40.0%
!! TGGATTTGTTGATTTCGTGC+AGG - chr5.4:77171638-77171657 MS.gene020921:CDS 40.0%
AAGATGCCTGTGGTTGGAAT+GGG - chr5.4:77171160-77171179 MS.gene020921:CDS 45.0%
ATTGACTGCAGGAAGAACAG+TGG + chr5.4:77171881-77171900 None:intergenic 45.0%
CAATTGAAGATGCCTGTGGT+TGG - chr5.4:77171154-77171173 MS.gene020921:CDS 45.0%
CAGAGGACCAAATGAGGTTA+TGG - chr5.4:77172098-77172117 MS.gene020921:CDS 45.0%
TTCAGAACAAGCTCTTGGTG+AGG - chr5.4:77171282-77171301 MS.gene020921:CDS 45.0%
TTTGATACTGCTGCTGCCTA+TGG - chr5.4:77171259-77171278 MS.gene020921:CDS 45.0%
! GGTTCTTTTGTTGCCATGCA+AGG + chr5.4:77172011-77172030 None:intergenic 45.0%
! GTGTTGGTAAGAACCTTGGT+TGG + chr5.4:77171127-77171146 None:intergenic 45.0%
!! GAAAGGTGTTTGGGAATCCA+TGG - chr5.4:77171771-77171790 MS.gene020921:CDS 45.0%
!! TCTTGGTGAGGCTTTGAAAG+AGG - chr5.4:77171294-77171313 MS.gene020921:CDS 45.0%
AGGTGGAGATGAACCTTGCA+TGG - chr5.4:77171995-77172014 MS.gene020921:intron 50.0%
AGGTGTTTGGGAATCCATGG+AGG - chr5.4:77171774-77171793 MS.gene020921:CDS 50.0%
CTGCTTGCACCTTTCCTCAA+CGG + chr5.4:77172081-77172100 None:intergenic 50.0%
GAAGATGCCTGTGGTTGGAA+TGG - chr5.4:77171159-77171178 MS.gene020921:CDS 50.0%
TCATAGAGGTCATTGAGGCC+TGG + chr5.4:77172345-77172364 None:intergenic 50.0%
TGCAAGCAGAGGACCAAATG+AGG - chr5.4:77172092-77172111 MS.gene020921:CDS 50.0%
! TAACTGCGTTTTCGCCGTTG+AGG - chr5.4:77172064-77172083 MS.gene020921:CDS 50.0%
! TGGCCACTTAGTTCTCAACC+TGG - chr5.4:77171700-77171719 MS.gene020921:CDS 50.0%
! TTGAGGAAAGGTGCAAGCAG+AGG - chr5.4:77172081-77172100 MS.gene020921:CDS 50.0%
GCTGATCCCATTCCAACCAC+AGG + chr5.4:77171169-77171188 None:intergenic 55.0%
! GCGTTTTCGCCGTTGAGGAA+AGG - chr5.4:77172069-77172088 MS.gene020921:CDS 55.0%
!! TCATTGAGGCCTGGCTTGGT+TGG + chr5.4:77172336-77172355 None:intergenic 55.0%
ATCCCTGGACCAACCAAGCC+AGG - chr5.4:77172324-77172343 MS.gene020921:CDS 60.0%
GAGGTCATTGAGGCCTGGCT+TGG + chr5.4:77172340-77172359 None:intergenic 60.0%
!! AGGCCTGGCTTGGTTGGTCC+AGG + chr5.4:77172330-77172349 None:intergenic 65.0%
!! GGCCTGGCTTGGTTGGTCCA+GGG + chr5.4:77172329-77172348 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 77171106 77172371 77171106 ID=MS.gene020921
chr5.4 mRNA 77171106 77172371 77171106 ID=MS.gene020921.t1;Parent=MS.gene020921
chr5.4 exon 77172052 77172371 77172052 ID=MS.gene020921.t1.exon1;Parent=MS.gene020921.t1
chr5.4 CDS 77172052 77172371 77172052 ID=cds.MS.gene020921.t1;Parent=MS.gene020921.t1
chr5.4 exon 77171574 77171817 77171574 ID=MS.gene020921.t1.exon2;Parent=MS.gene020921.t1
chr5.4 CDS 77171574 77171817 77171574 ID=cds.MS.gene020921.t1;Parent=MS.gene020921.t1
chr5.4 exon 77171106 77171480 77171106 ID=MS.gene020921.t1.exon3;Parent=MS.gene020921.t1
chr5.4 CDS 77171106 77171480 77171106 ID=cds.MS.gene020921.t1;Parent=MS.gene020921.t1
Gene Sequence

>MS.gene020921

ATGGGTAGTGTTGAAATCCCAACCAAGGTTCTTACCAACACTTCTAGTCAATTGAAGATGCCTGTGGTTGGAATGGGATCAGCACCTGACTTCACATGTAAGAAAGACACAAAAGATGCAATCATTGAAGCCATCAAACAAGGTTATAGACACTTTGATACTGCTGCTGCCTATGGTTCAGAACAAGCTCTTGGTGAGGCTTTGAAAGAGGCAATTGAACTTGGTCTTGTCACTAGAAATGAGCTTTTTGTTACTTCCAAACTTTGGGTTACTGAAAATCATCCTCATCTTGTTATTCCTGCTCTTCAAAAATCTCTCAAGTAAGCAAATTCTAATGTTACACTCATTTAGAGTATATTTAGATGACATTTGGTTCTATAGTGACGAAATTAATTTTTAATCAATTGATTTTGTAAAATATATTTCATTAAAAGTAAGATGAGTGTTAAATTGTTTTTTATTTAGATATATTCACGTAAATATCAATTGAAGAACAAAAGTGAATCAAAACATACACTTATTGAGATTAAACTGGATTTGTTGATTTCGTGCAGGACTCTTCAACTAGACTACTTGGACTTGTACTTGATTCATTGGCCACTTAGTTCTCAACCTGGAAAGTTTACATTTCCAATTGATGTGGCAGATCTCTTACCATTTGATGTGAAAGGTGTTTGGGAATCCATGGAGGAAAGCTTGAAACTTGGACTCACTAAAGCTATTGGAGTTAGTAACTTCTCTGTCAAGAAACTTGAAAATCTTCTCTCTGTTGCCACTGTTCTTCCTGCAGTCAATCAAGTAATTCATCAACTAAACCTAAGTTTTGATTAACTTCTTGTAAACTTCTTAATTGTTAACATGTACTTATACAATGAACTCATATATTTACAGGTGGAGATGAACCTTGCATGGCAACAAAAGAACCTTAGAGAGTTTTGCAATGCAAATGGAATAGTGTTAACTGCGTTTTCGCCGTTGAGGAAAGGTGCAAGCAGAGGACCAAATGAGGTTATGGAGAATGATATGCTTAAAGAGATTGCAGATGCACATGGAAAGTCTGTTGCACAAATTTCTCTAAGATGGCTATATGAACAAGGAGTCACTTTTGTTCCCAAGAGTTATGATAAGGAAAGAATGAACCAAAATTTGTGTATCTTTGATTGGTCATTGACAAAAGAAGATCATGAGAAAATCGATCAAATTAAGCAAAATCGTTTGATCCCTGGACCAACCAAGCCAGGCCTCAATGACCTCTATGATGACTAA
Protein sequence

>MS.gene020921.t1

MGSVEIPTKVLTNTSSQLKMPVVGMGSAPDFTCKKDTKDAIIEAIKQGYRHFDTAAAYGSEQALGEALKEAIELGLVTRNELFVTSKLWVTENHPHLVIPALQKSLKTLQLDYLDLYLIHWPLSSQPGKFTFPIDVADLLPFDVKGVWESMEESLKLGLTKAIGVSNFSVKKLENLLSVATVLPAVNQVEMNLAWQQKNLREFCNANGIVLTAFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPGPTKPGLNDLYDD