AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87111

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87111.t1 MTR_4g036845 97.460 315 8 0 1 315 1 315 0.0 646
MS.gene87111.t1 MTR_4g072320 46.057 317 162 5 2 315 5 315 1.52e-95 285
MS.gene87111.t1 MTR_4g072350 45.426 317 168 5 2 314 5 320 6.76e-90 271
MS.gene87111.t1 MTR_6g073110 45.338 311 165 4 6 312 9 318 2.64e-89 270
MS.gene87111.t1 MTR_8g070095 43.269 312 171 3 8 315 10 319 8.34e-88 266
MS.gene87111.t1 MTR_4g072360 45.079 315 168 5 2 312 5 318 1.65e-87 265
MS.gene87111.t1 MTR_8g070115 42.947 319 171 5 3 315 2 315 8.02e-86 261
MS.gene87111.t1 MTR_0374s0050 43.791 306 157 6 14 314 20 315 2.73e-85 259
MS.gene87111.t1 MTR_7g063580 40.810 321 169 5 4 315 46 354 5.35e-78 242
MS.gene87111.t1 MTR_4g021350 41.801 311 173 4 7 315 9 313 2.82e-76 236
MS.gene87111.t1 MTR_3g083130 39.672 305 170 7 14 314 21 315 4.16e-75 233
MS.gene87111.t1 MTR_4g021410 40.968 310 164 5 8 314 11 304 6.10e-69 217
MS.gene87111.t1 MTR_4g021450 40.323 310 166 5 8 314 11 304 3.58e-67 213
MS.gene87111.t1 MTR_4g021350 43.116 276 148 5 7 280 9 277 9.56e-66 208
MS.gene87111.t1 MTR_5g097910 40.000 305 170 6 12 313 18 312 4.64e-65 207
MS.gene87111.t1 MTR_5g097940 40.000 305 170 6 12 313 18 312 4.64e-65 207
MS.gene87111.t1 MTR_5g097900 40.702 285 164 4 12 293 18 300 8.97e-65 206
MS.gene87111.t1 MTR_4g021410 42.086 278 146 3 39 314 3 267 9.50e-65 205
MS.gene87111.t1 MTR_7g070500 38.782 312 178 8 8 314 17 320 6.53e-61 197
MS.gene87111.t1 MTR_4g072060 38.158 304 170 5 6 299 3 298 8.59e-60 194
MS.gene87111.t1 MTR_7g070500 39.502 281 159 6 8 285 17 289 1.93e-53 177
MS.gene87111.t1 MTR_4g021410 40.664 241 128 3 76 314 1 228 2.36e-49 164
MS.gene87111.t1 MTR_4g021450 40.496 242 127 4 76 314 1 228 1.18e-48 162
MS.gene87111.t1 MTR_4g021450 41.989 181 99 2 135 314 10 185 8.65e-45 151
MS.gene87111.t1 MTR_6g072650 38.776 147 67 2 168 314 13 136 6.28e-28 105
MS.gene87111.t1 MTR_6g072710 44.248 113 63 0 183 295 1 113 3.99e-25 97.8
MS.gene87111.t1 MTR_3g449790 25.460 326 172 12 12 285 45 351 6.22e-18 83.6
MS.gene87111.t1 MTR_3g449790 24.540 326 169 11 12 285 45 345 2.76e-17 81.6
MS.gene87111.t1 MTR_7g114980 26.087 322 173 12 20 298 35 334 6.75e-16 77.8
MS.gene87111.t1 MTR_1g056790 94.118 34 2 0 263 296 1 34 1.92e-14 67.0
MS.gene87111.t1 MTR_7g114990 25.667 300 164 10 20 282 37 314 8.22e-14 71.6
MS.gene87111.t1 MTR_7g021850 25.994 327 167 14 11 288 13 313 1.16e-13 70.9
MS.gene87111.t1 MTR_1g102750 24.776 335 174 12 11 294 14 321 4.02e-13 69.3
MS.gene87111.t1 MTR_7g114980 26.812 276 139 11 20 254 35 288 3.70e-12 66.2
MS.gene87111.t1 MTR_7g021680 25.231 325 169 13 11 287 13 311 7.61e-12 65.5
MS.gene87111.t1 MTR_7g021820 25.231 325 169 13 11 287 13 311 7.61e-12 65.5
MS.gene87111.t1 MTR_6g086545 50.000 56 28 0 64 119 37 92 7.24e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87111.t1 AT5G62420 68.038 316 100 1 1 315 1 316 2.30e-164 460
MS.gene87111.t1 AT2G37770 44.373 311 167 3 7 315 9 315 3.43e-91 274
MS.gene87111.t1 AT2G37790 43.730 311 166 5 8 315 10 314 4.29e-86 261
MS.gene87111.t1 AT1G59960 42.071 309 171 7 13 315 20 326 6.75e-86 261
MS.gene87111.t1 AT2G37760 41.935 310 170 3 8 315 10 311 6.01e-82 251
MS.gene87111.t1 AT2G37760 41.935 310 170 3 8 315 10 311 6.01e-82 251
MS.gene87111.t1 AT3G53880 41.801 311 175 3 7 315 9 315 4.78e-80 246
MS.gene87111.t1 AT1G59950 41.558 308 172 7 14 315 15 320 8.25e-75 233
MS.gene87111.t1 AT2G37760 40.909 286 159 3 8 291 10 287 1.55e-74 231
MS.gene87111.t1 AT2G37760 40.909 286 159 3 8 291 10 287 1.55e-74 231
MS.gene87111.t1 AT2G37760 43.273 275 145 4 8 280 10 275 2.15e-74 231
MS.gene87111.t1 AT2G21250 39.145 304 167 6 6 299 3 298 1.76e-70 221
MS.gene87111.t1 AT5G01670 38.462 312 181 7 7 314 17 321 2.28e-70 221
MS.gene87111.t1 AT2G21260 38.689 305 169 6 6 300 3 299 9.51e-70 219
MS.gene87111.t1 AT3G53880 40.493 284 163 3 7 288 9 288 1.91e-67 214
MS.gene87111.t1 AT2G37760 42.308 260 146 2 58 315 8 265 3.21e-66 209
MS.gene87111.t1 AT2G37760 42.308 260 146 2 58 315 8 265 3.21e-66 209
MS.gene87111.t1 AT5G01670 35.693 339 180 8 7 314 17 348 3.78e-66 211
MS.gene87111.t1 AT2G21250 39.485 233 129 5 6 228 3 233 2.01e-53 175
MS.gene87111.t1 AT2G37770 43.000 200 108 3 7 204 9 204 1.00e-50 169
MS.gene87111.t1 AT2G21260 38.197 233 128 5 78 300 5 231 3.15e-47 159
MS.gene87111.t1 AT1G06690 26.471 306 165 11 29 293 85 371 3.54e-19 87.4
MS.gene87111.t1 AT5G53580 24.038 312 178 9 17 283 50 347 3.44e-14 72.8
MS.gene87111.t1 AT1G60710 25.705 319 170 11 22 298 33 326 7.08e-14 71.6
MS.gene87111.t1 AT1G60750 24.601 313 184 12 22 308 34 320 2.96e-12 67.0
MS.gene87111.t1 AT1G60690 24.566 346 185 14 2 297 6 325 4.75e-12 66.2
MS.gene87111.t1 AT1G60730 24.611 321 179 11 22 302 33 330 5.21e-12 66.2
MS.gene87111.t1 AT1G60730 24.540 326 193 11 22 302 33 350 7.37e-12 65.9

Find 79 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGATGACTTTGAAAATT+TGG 0.094815 4.3:+63169540 MS.gene87111:CDS
TTTGACACGGCCAAAATTTA+TGG 0.143750 4.3:+63168782 MS.gene87111:CDS
AAGGCAATTGATTGTATAAT+TGG 0.200651 4.3:-63170202 None:intergenic
CATGTAGATTGCCTTATTTA+TGG 0.211532 4.3:-63168828 None:intergenic
TCTGTTGTCTTCTTATCATT+TGG 0.211759 4.3:-63168191 None:intergenic
GAATCTGGACATGGACTATT+TGG 0.224464 4.3:+63169456 MS.gene87111:CDS
TGAAAGAATGGAGGAAAATA+TGG 0.239865 4.3:+63170618 MS.gene87111:CDS
TGGCTCAGAACCATAAATTT+TGG 0.248086 4.3:-63168792 None:intergenic
CTGTTGTCTTCTTATCATTT+GGG 0.296041 4.3:-63168190 None:intergenic
TGAAATTTAATTACCTGTGC+TGG 0.326139 4.3:-63170241 None:intergenic
ACAATGCCTCTCATTGGATT+TGG 0.330693 4.3:+63168155 MS.gene87111:CDS
ACAAAACAATTGAAGAACTT+TGG 0.349387 4.3:+63170749 MS.gene87111:CDS
ATTAGGCAATGCCATAAATA+AGG 0.350442 4.3:+63168817 MS.gene87111:CDS
CAAAACAATTGAAGAACTTT+GGG 0.354545 4.3:+63170750 MS.gene87111:CDS
ATTTGAAGGTTGCATTGAAA+TGG 0.361915 4.3:+63170551 MS.gene87111:intron
TGGGGTTGCTTTGTGTTTGA+AGG 0.388559 4.3:-63170221 None:intergenic
GGCATTACAATGCCTCTCAT+TGG 0.395160 4.3:+63168149 MS.gene87111:CDS
TACACCTCCAGTTGTTAATC+AGG 0.396062 4.3:+63169678 MS.gene87111:CDS
TTTGAAGGTTGCATTGAAAT+GGG 0.414907 4.3:+63170552 MS.gene87111:intron
TTAATTACCTGATTAACAAC+TGG 0.420192 4.3:-63169685 None:intergenic
TACTCACCACTTGGTGGACC+AGG 0.421010 4.3:+63170145 MS.gene87111:CDS
GAAATTTAATTACCTGTGCT+GGG 0.421840 4.3:-63170240 None:intergenic
AAGTCATCTTCATTAGGAAC+AGG 0.433658 4.3:-63169529 None:intergenic
TTTATGGCATTGCCTAATGC+TGG 0.448486 4.3:-63168812 None:intergenic
CATAAATAAGGCAATCTACA+TGG 0.451120 4.3:+63168829 MS.gene87111:CDS
TTGGTGGACCAGGGAATGCA+TGG 0.463271 4.3:+63170155 MS.gene87111:CDS
TATGGTTCTGAGCCAGCATT+AGG 0.464141 4.3:+63168800 MS.gene87111:CDS
GTTGATCCCCATGCATTCCC+TGG 0.477901 4.3:-63170163 None:intergenic
TTTCTTGACATCCAAATTGT+GGG 0.481755 4.3:+63168874 MS.gene87111:CDS
GCCTGTAAAGTTAAAACCAT+GGG 0.488120 4.3:+63169498 MS.gene87111:CDS
GAAAGAATGGAGGAAAATAT+GGG 0.502628 4.3:+63170619 MS.gene87111:CDS
GGGATCATTTGATCTAAACT+TGG 0.506454 4.3:+63170639 MS.gene87111:CDS
GTCTTGAAATGGGGTTATGT+AGG 0.513056 4.3:+63169596 MS.gene87111:CDS
AAGTTTCAATGATGAAAGAA+TGG 0.517652 4.3:+63170606 MS.gene87111:CDS
TTAATGCAGCAACAGGATCA+TGG 0.518812 4.3:-63168907 None:intergenic
TGGATATGTACCTAGTGCAT+TGG 0.523656 4.3:+63169476 MS.gene87111:CDS
TGTCTTCTTATCATTTGGGT+AGG 0.528221 4.3:-63168186 None:intergenic
ATGGGGTTATGTAGGTCCAT+TGG 0.529847 4.3:+63169604 MS.gene87111:CDS
ATTTGGATCTTGAGACAACA+TGG 0.540374 4.3:+63169557 MS.gene87111:CDS
ACTTTACAGGCCAATGCACT+AGG 0.547784 4.3:-63169486 None:intergenic
TCTACATGGGAGAAGTAAAG+AGG 0.554077 4.3:+63168843 MS.gene87111:CDS
AATCATGTGCGTTTGAATTG+TGG 0.556437 4.3:+63168128 MS.gene87111:CDS
TGGTGGACCAGGGAATGCAT+GGG 0.560104 4.3:+63170156 MS.gene87111:CDS
TTGAAATGGGGATTATCAAA+GGG 0.560330 4.3:+63170565 MS.gene87111:CDS
ATTGAAATGGGGATTATCAA+AGG 0.560489 4.3:+63170564 MS.gene87111:CDS
AAGAAATGAAGATTATGAGA+GGG 0.564196 4.3:+63170695 MS.gene87111:CDS
TGAGACAACATGGGTAGGGA+TGG 0.572572 4.3:+63169567 MS.gene87111:CDS
TTCTTGACATCCAAATTGTG+GGG 0.578334 4.3:+63168875 MS.gene87111:CDS
GGAACAGGATAGTTAACCCA+TGG 0.578853 4.3:-63169514 None:intergenic
TGGTCCACCAAGTGGTGAGT+AGG 0.583500 4.3:-63170143 None:intergenic
GATCTTGAGACAACATGGGT+AGG 0.585515 4.3:+63169562 MS.gene87111:CDS
ACACAAAGCAACCCCAGCAC+AGG 0.586444 4.3:+63170228 MS.gene87111:CDS
TTGAAGGTTGCATTGAAATG+GGG 0.588735 4.3:+63170553 MS.gene87111:intron
GAAGAAATGAAGATTATGAG+AGG 0.591725 4.3:+63170694 MS.gene87111:CDS
AGAGAAATTACTAACTCCAA+TGG 0.591742 4.3:-63169620 None:intergenic
ATCTTGAGACAACATGGGTA+GGG 0.592828 4.3:+63169563 MS.gene87111:CDS
ATAAATAAGGCAATCTACAT+GGG 0.593397 4.3:+63168830 MS.gene87111:CDS
TGCAGTCCACAATGCCCTTG+AGG 0.593505 4.3:+63168217 MS.gene87111:CDS
TCTCTAGCAACAAGATTGAG+TGG 0.593774 4.3:+63169638 MS.gene87111:CDS
TTTCAATGATGAAAGAATGG+AGG 0.594754 4.3:+63170609 MS.gene87111:CDS
GGCCTGTAAAGTTAAAACCA+TGG 0.600838 4.3:+63169497 MS.gene87111:CDS
CTACATGGGAGAAGTAAAGA+GGG 0.601362 4.3:+63168844 MS.gene87111:CDS
AGAAATGAAGATTATGAGAG+GGG 0.602586 4.3:+63170696 MS.gene87111:CDS
GTAAGTGCCTACTCACCACT+TGG 0.619442 4.3:+63170136 MS.gene87111:CDS
AGTGCCTACTCACCACTTGG+TGG 0.619529 4.3:+63170139 MS.gene87111:CDS
AGCTTTCTCTGCCTCCACAT+AGG 0.620551 4.3:-63170082 None:intergenic
TCTTGACATCCAAATTGTGG+GGG 0.621026 4.3:+63168876 MS.gene87111:CDS
AGGTGGAAATGCATCCTATG+TGG 0.626109 4.3:+63170068 MS.gene87111:intron
ATTACCTGATTAACAACTGG+AGG 0.626966 4.3:-63169682 None:intergenic
AGAAAGCTGAGAAAGACATG+TGG 0.633349 4.3:+63170097 MS.gene87111:CDS
GATTAAGGTTAGTACCTCAA+GGG 0.643115 4.3:-63168231 None:intergenic
AAATTTAATTACCTGTGCTG+GGG 0.643136 4.3:-63170239 None:intergenic
TAAGTGCCAAATCCAATGAG+AGG 0.655261 4.3:-63168161 None:intergenic
TGGAAATGCATCCTATGTGG+AGG 0.657202 4.3:+63170071 MS.gene87111:CDS
GGTGGACCAGGGAATGCATG+GGG 0.659494 4.3:+63170157 MS.gene87111:CDS
GCATTCCCTGGTCCACCAAG+TGG 0.660549 4.3:-63170151 None:intergenic
TTTGGATCTTGAGACAACAT+GGG 0.665864 4.3:+63169558 MS.gene87111:CDS
ACTCACCACTTGGTGGACCA+GGG 0.670084 4.3:+63170146 MS.gene87111:CDS
TTAGTACCTCAAGGGCATTG+TGG 0.678400 4.3:-63168223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTATTCTTAAATTTCG+TGG - chr4.3:63169247-63169266 None:intergenic 15.0%
!! TAATATCTTGATATATACTT+AGG + chr4.3:63170048-63170067 MS.gene87111:intron 15.0%
!! TATATTTGTGTTCAATAATA+AGG - chr4.3:63168728-63168747 None:intergenic 15.0%
!!! CTAGTTTAAAATATTAATAG+CGG - chr4.3:63168378-63168397 None:intergenic 15.0%
!!! TATATTTGAGATTGAAAAAT+TGG + chr4.3:63170672-63170691 MS.gene87111:CDS 15.0%
!!! TGAGATTTAATATTGATTTT+TGG + chr4.3:63169410-63169429 MS.gene87111:intron 15.0%
!!! TGTAAAAAAAAATTCTAAGA+TGG + chr4.3:63169010-63169029 MS.gene87111:intron 15.0%
!!! TTTTTTTACATGAAATGAAA+AGG - chr4.3:63169000-63169019 None:intergenic 15.0%
!! ATGAACATGTATTTATAAGT+GGG + chr4.3:63169172-63169191 MS.gene87111:intron 20.0%
!! TAATCAGGTAATTAACTAAT+TGG + chr4.3:63169693-63169712 MS.gene87111:intron 20.0%
!! TATAAGAAAAAACCATTTGT+AGG + chr4.3:63169752-63169771 MS.gene87111:intron 20.0%
!! TGTATCATTAGTAAAATGAA+TGG + chr4.3:63170483-63170502 MS.gene87111:intron 20.0%
!! TGTCAAATTTATTTGAACAT+TGG - chr4.3:63170412-63170431 None:intergenic 20.0%
!!! AAAAACAGAGATTAATTTGA+TGG + chr4.3:63168550-63168569 MS.gene87111:intron 20.0%
!!! ATTAATTTAAAATACAAGGG+AGG - chr4.3:63168636-63168655 None:intergenic 20.0%
! AACAAATGTGTATGTGATTA+AGG - chr4.3:63168249-63168268 None:intergenic 25.0%
! AAGAAATGAAGATTATGAGA+GGG + chr4.3:63170695-63170714 MS.gene87111:CDS 25.0%
! AAGGCAATTGATTGTATAAT+TGG - chr4.3:63170205-63170224 None:intergenic 25.0%
! AAGTTTCAATGATGAAAGAA+TGG + chr4.3:63170606-63170625 MS.gene87111:CDS 25.0%
! ACAAAACAATTGAAGAACTT+TGG + chr4.3:63170749-63170768 MS.gene87111:CDS 25.0%
! ATAAATAAGGCAATCTACAT+GGG + chr4.3:63168830-63168849 MS.gene87111:CDS 25.0%
! ATGGAAAAATGTCTTGAAAT+GGG + chr4.3:63169586-63169605 MS.gene87111:CDS 25.0%
! CAAAACAATTGAAGAACTTT+GGG + chr4.3:63170750-63170769 MS.gene87111:CDS 25.0%
! CATGAACATGTATTTATAAG+TGG + chr4.3:63169171-63169190 MS.gene87111:intron 25.0%
! GAAAAAATGAGTAAATGAAG+TGG + chr4.3:63169986-63170005 MS.gene87111:intron 25.0%
! TATCTTGATATATACTTAGG+TGG + chr4.3:63170051-63170070 MS.gene87111:intron 25.0%
! TGAACATGTATTTATAAGTG+GGG + chr4.3:63169173-63169192 MS.gene87111:intron 25.0%
! TGATACAAATGCAACTAATT+TGG - chr4.3:63170470-63170489 None:intergenic 25.0%
! TTAATTACCTGATTAACAAC+TGG - chr4.3:63169688-63169707 None:intergenic 25.0%
! TTATTAGATCAACCTACAAA+TGG - chr4.3:63169767-63169786 None:intergenic 25.0%
!! AGAAACTTTTGGCAAAAAAA+GGG + chr4.3:63168470-63168489 MS.gene87111:intron 25.0%
!! TGAAGATGACTTTGAAAATT+TGG + chr4.3:63169540-63169559 MS.gene87111:CDS 25.0%
!! TTTAAAATATTAATAGCGGC+AGG - chr4.3:63168374-63168393 None:intergenic 25.0%
!! TTTTACTTGAATGCAAAAAC+AGG + chr4.3:63169434-63169453 MS.gene87111:intron 25.0%
!! TTTTCAAAGTCATCTTCATT+AGG - chr4.3:63169538-63169557 None:intergenic 25.0%
!!! AAGTTCTTCAATTGTTTTGT+AGG - chr4.3:63170749-63170768 None:intergenic 25.0%
!!! AGTTCTTCAATTGTTTTGTA+GGG - chr4.3:63170748-63170767 None:intergenic 25.0%
!!! TAGGCATCAGATTTTAATTT+TGG - chr4.3:63169350-63169369 None:intergenic 25.0%
AAAGATAGAGACCAGTTTAA+TGG - chr4.3:63168329-63168348 None:intergenic 30.0%
AAATTTAATTACCTGTGCTG+GGG - chr4.3:63170242-63170261 None:intergenic 30.0%
AGAAATGAAGATTATGAGAG+GGG + chr4.3:63170696-63170715 MS.gene87111:CDS 30.0%
AGAGAAATTACTAACTCCAA+TGG - chr4.3:63169623-63169642 None:intergenic 30.0%
AGTCTACTGATATTGACATT+AGG - chr4.3:63169953-63169972 None:intergenic 30.0%
ATATATGTGTGTATGTATGC+AGG + chr4.3:63168745-63168764 MS.gene87111:intron 30.0%
ATTAGGCAATGCCATAAATA+AGG + chr4.3:63168817-63168836 MS.gene87111:CDS 30.0%
ATTGAAATGGGGATTATCAA+AGG + chr4.3:63170564-63170583 MS.gene87111:CDS 30.0%
ATTTGAAGGTTGCATTGAAA+TGG + chr4.3:63170551-63170570 MS.gene87111:intron 30.0%
CAAAAGATTTGTCACACAAT+AGG - chr4.3:63169369-63169388 None:intergenic 30.0%
CATAAATAAGGCAATCTACA+TGG + chr4.3:63168829-63168848 MS.gene87111:CDS 30.0%
CTGTTGTCTTCTTATCATTT+GGG - chr4.3:63168193-63168212 None:intergenic 30.0%
GAAAGAATGGAGGAAAATAT+GGG + chr4.3:63170619-63170638 MS.gene87111:CDS 30.0%
GAAATTTAATTACCTGTGCT+GGG - chr4.3:63170243-63170262 None:intergenic 30.0%
GAAGAAATGAAGATTATGAG+AGG + chr4.3:63170694-63170713 MS.gene87111:CDS 30.0%
GATGGAAAAATGTCTTGAAA+TGG + chr4.3:63169585-63169604 MS.gene87111:CDS 30.0%
GTCAAATGACAAAAACATGA+CGG + chr4.3:63168436-63168455 MS.gene87111:intron 30.0%
GTCTACTGATATTGACATTA+GGG - chr4.3:63169952-63169971 None:intergenic 30.0%
TAACGAGTAAACCATTAAAC+TGG + chr4.3:63168315-63168334 MS.gene87111:intron 30.0%
TAATGTCAATATCAGTAGAC+TGG + chr4.3:63169952-63169971 MS.gene87111:intron 30.0%
TCTGTTGTCTTCTTATCATT+TGG - chr4.3:63168194-63168213 None:intergenic 30.0%
TGAAAGAATGGAGGAAAATA+TGG + chr4.3:63170618-63170637 MS.gene87111:CDS 30.0%
TGAAATTTAATTACCTGTGC+TGG - chr4.3:63170244-63170263 None:intergenic 30.0%
TGGAAAAATGTCTTGAAATG+GGG + chr4.3:63169587-63169606 MS.gene87111:CDS 30.0%
TTGAAATGGGGATTATCAAA+GGG + chr4.3:63170565-63170584 MS.gene87111:CDS 30.0%
TTGCCAAAAGTTTCTCAATT+AGG - chr4.3:63168465-63168484 None:intergenic 30.0%
TTTCAATGATGAAAGAATGG+AGG + chr4.3:63170609-63170628 MS.gene87111:CDS 30.0%
TTTCTTGACATCCAAATTGT+GGG + chr4.3:63168874-63168893 MS.gene87111:CDS 30.0%
TTTGAAGGTTGCATTGAAAT+GGG + chr4.3:63170552-63170571 MS.gene87111:intron 30.0%
! AGACCTAATTGAGAAACTTT+TGG + chr4.3:63168459-63168478 MS.gene87111:intron 30.0%
! GAGAAACTTTTGGCAAAAAA+AGG + chr4.3:63168469-63168488 MS.gene87111:intron 30.0%
! TTTTCTTGACATCCAAATTG+TGG + chr4.3:63168873-63168892 MS.gene87111:CDS 30.0%
!! CATGTAGATTGCCTTATTTA+TGG - chr4.3:63168831-63168850 None:intergenic 30.0%
!! GACTATGATCTTTGATTTGA+AGG + chr4.3:63170537-63170556 MS.gene87111:intron 30.0%
AAGTCATCTTCATTAGGAAC+AGG - chr4.3:63169532-63169551 None:intergenic 35.0%
AAGTCTTAAGCAAACACCAT+CGG - chr4.3:63169892-63169911 None:intergenic 35.0%
AATCATGTGCGTTTGAATTG+TGG + chr4.3:63168128-63168147 MS.gene87111:CDS 35.0%
ACTGAATGAAGTAACCCATT+TGG + chr4.3:63169860-63169879 MS.gene87111:intron 35.0%
ATTACCTGATTAACAACTGG+AGG - chr4.3:63169685-63169704 None:intergenic 35.0%
ATTTGGATCTTGAGACAACA+TGG + chr4.3:63169557-63169576 MS.gene87111:CDS 35.0%
CTAACTCAATCTCTCAAAAC+CGG - chr4.3:63169218-63169237 None:intergenic 35.0%
CTGAATGAAGTAACCCATTT+GGG + chr4.3:63169861-63169880 MS.gene87111:intron 35.0%
GATTAAGGTTAGTACCTCAA+GGG - chr4.3:63168234-63168253 None:intergenic 35.0%
GCCTGTAAAGTTAAAACCAT+GGG + chr4.3:63169498-63169517 MS.gene87111:CDS 35.0%
GGGATCATTTGATCTAAACT+TGG + chr4.3:63170639-63170658 MS.gene87111:CDS 35.0%
TGAATGCAAAAACAGGAATC+TGG + chr4.3:63169441-63169460 MS.gene87111:intron 35.0%
TGATTAAGGTTAGTACCTCA+AGG - chr4.3:63168235-63168254 None:intergenic 35.0%
TGTCTTCTTATCATTTGGGT+AGG - chr4.3:63168189-63168208 None:intergenic 35.0%
TGTGATCCAATAGATCTTAG+CGG - chr4.3:63169047-63169066 None:intergenic 35.0%
TTCTTGACATCCAAATTGTG+GGG + chr4.3:63168875-63168894 MS.gene87111:CDS 35.0%
TTGAAGGTTGCATTGAAATG+GGG + chr4.3:63170553-63170572 MS.gene87111:intron 35.0%
TTTGACACGGCCAAAATTTA+TGG + chr4.3:63168782-63168801 MS.gene87111:CDS 35.0%
TTTGGATCTTGAGACAACAT+GGG + chr4.3:63169558-63169577 MS.gene87111:CDS 35.0%
! AACCATTGGTTGTTGCAATT+TGG - chr4.3:63169319-63169338 None:intergenic 35.0%
! TGCTATCAGGTTTTCACTAT+CGG + chr4.3:63169067-63169086 MS.gene87111:intron 35.0%
! TGGCTCAGAACCATAAATTT+TGG - chr4.3:63168795-63168814 None:intergenic 35.0%
!! AATTGTTTTGTAGGGACTTG+TGG - chr4.3:63170740-63170759 None:intergenic 35.0%
!! CATGTTTTTGTCATTTGACG+AGG - chr4.3:63168435-63168454 None:intergenic 35.0%
!! GGGTTATAGACATTTTGACA+CGG + chr4.3:63168769-63168788 MS.gene87111:CDS 35.0%
!!! ACCCATGGTTTTAACTTTAC+AGG - chr4.3:63169502-63169521 None:intergenic 35.0%
!!! GTTTGTTTTAATGCAGCAAC+AGG - chr4.3:63168917-63168936 None:intergenic 35.0%
!!! TTTTGGTAGTGACAAACCAT+TGG - chr4.3:63169333-63169352 None:intergenic 35.0%
ACAATGCCTCTCATTGGATT+TGG + chr4.3:63168155-63168174 MS.gene87111:CDS 40.0%
ACCATCGGATTATCCCAAAT+GGG - chr4.3:63169877-63169896 None:intergenic 40.0%
AGAAAGCTGAGAAAGACATG+TGG + chr4.3:63170097-63170116 MS.gene87111:CDS 40.0%
ATCTTGAGACAACATGGGTA+GGG + chr4.3:63169563-63169582 MS.gene87111:CDS 40.0%
ATGTGTGTATGTATGCAGGT+GGG + chr4.3:63168749-63168768 MS.gene87111:intron 40.0%
CAAAAACAGGAATCTGGACA+TGG + chr4.3:63169447-63169466 MS.gene87111:intron 40.0%
CACCAAATTGCAACAACCAA+TGG + chr4.3:63169314-63169333 MS.gene87111:intron 40.0%
CTACATGGGAGAAGTAAAGA+GGG + chr4.3:63168844-63168863 MS.gene87111:CDS 40.0%
CTATTGGATCACATGCTATC+AGG + chr4.3:63169054-63169073 MS.gene87111:intron 40.0%
GCTTGGTTCATGGACATAAA+TGG - chr4.3:63169101-63169120 None:intergenic 40.0%
GGCCTGTAAAGTTAAAACCA+TGG + chr4.3:63169497-63169516 MS.gene87111:CDS 40.0%
GGTTCATGGACATAAATGGT+GGG - chr4.3:63169097-63169116 None:intergenic 40.0%
GTCTTGAAATGGGGTTATGT+AGG + chr4.3:63169596-63169615 MS.gene87111:CDS 40.0%
TAAGTGCCAAATCCAATGAG+AGG - chr4.3:63168164-63168183 None:intergenic 40.0%
TACACCTCCAGTTGTTAATC+AGG + chr4.3:63169678-63169697 MS.gene87111:CDS 40.0%
TATGTGTGTATGTATGCAGG+TGG + chr4.3:63168748-63168767 MS.gene87111:intron 40.0%
TCTACATGGGAGAAGTAAAG+AGG + chr4.3:63168843-63168862 MS.gene87111:CDS 40.0%
TCTCTAGCAACAAGATTGAG+TGG + chr4.3:63169638-63169657 MS.gene87111:CDS 40.0%
TCTTGACATCCAAATTGTGG+GGG + chr4.3:63168876-63168895 MS.gene87111:CDS 40.0%
TGGATATGTACCTAGTGCAT+TGG + chr4.3:63169476-63169495 MS.gene87111:CDS 40.0%
TGGTTCATGGACATAAATGG+TGG - chr4.3:63169098-63169117 None:intergenic 40.0%
TTAATGCAGCAACAGGATCA+TGG - chr4.3:63168910-63168929 None:intergenic 40.0%
TTTATGGCATTGCCTAATGC+TGG - chr4.3:63168815-63168834 None:intergenic 40.0%
! GAATCTGGACATGGACTATT+TGG + chr4.3:63169456-63169475 MS.gene87111:CDS 40.0%
! GCTATCAGGTTTTCACTATC+GGG + chr4.3:63169068-63169087 MS.gene87111:intron 40.0%
! TTTTGTAGGGACTTGTGGTT+TGG - chr4.3:63170735-63170754 None:intergenic 40.0%
!!! CGGTTTTGAGAGATTGAGTT+AGG + chr4.3:63169216-63169235 MS.gene87111:intron 40.0%
ACAACCAACACTATTGGGCT+TGG - chr4.3:63169118-63169137 None:intergenic 45.0%
ACAATCCTCACCTCAGAAAC+CGG + chr4.3:63169196-63169215 MS.gene87111:intron 45.0%
ACTTTACAGGCCAATGCACT+AGG - chr4.3:63169489-63169508 None:intergenic 45.0%
AGGTGGAAATGCATCCTATG+TGG + chr4.3:63170068-63170087 MS.gene87111:intron 45.0%
ATGGGGTTATGTAGGTCCAT+TGG + chr4.3:63169604-63169623 MS.gene87111:CDS 45.0%
CACCATCGGATTATCCCAAA+TGG - chr4.3:63169878-63169897 None:intergenic 45.0%
CCCTCACAACCAACACTATT+GGG - chr4.3:63169123-63169142 None:intergenic 45.0%
GAGTCACCGCTAAGATCTAT+TGG + chr4.3:63169038-63169057 MS.gene87111:intron 45.0%
GATCTTGAGACAACATGGGT+AGG + chr4.3:63169562-63169581 MS.gene87111:CDS 45.0%
GGAACAGGATAGTTAACCCA+TGG - chr4.3:63169517-63169536 None:intergenic 45.0%
GGCATTACAATGCCTCTCAT+TGG + chr4.3:63168149-63168168 MS.gene87111:CDS 45.0%
TGAACCAAGCCCAATAGTGT+TGG + chr4.3:63169111-63169130 MS.gene87111:intron 45.0%
TGGAAATGCATCCTATGTGG+AGG + chr4.3:63170071-63170090 MS.gene87111:CDS 45.0%
! ACACTATTGGGCTTGGTTCA+TGG - chr4.3:63169111-63169130 None:intergenic 45.0%
! ACCCATTTGGGATAATCCGA+TGG + chr4.3:63169873-63169892 MS.gene87111:intron 45.0%
! TCTCTCAAAACCGGTTTCTG+AGG - chr4.3:63169209-63169228 None:intergenic 45.0%
! TGGGGTTGCTTTGTGTTTGA+AGG - chr4.3:63170224-63170243 None:intergenic 45.0%
!! CAATAGTGTTGGTTGTGAGG+GGG + chr4.3:63169122-63169141 MS.gene87111:intron 45.0%
!! CCAATAGTGTTGGTTGTGAG+GGG + chr4.3:63169121-63169140 MS.gene87111:intron 45.0%
!! CCCAATAGTGTTGGTTGTGA+GGG + chr4.3:63169120-63169139 MS.gene87111:intron 45.0%
!! TATGGTTCTGAGCCAGCATT+AGG + chr4.3:63168800-63168819 MS.gene87111:CDS 45.0%
!! TTAGTACCTCAAGGGCATTG+TGG - chr4.3:63168226-63168245 None:intergenic 45.0%
!!! TATATTAATTTAAAATACAA+GGG - chr4.3:63168639-63168658 None:intergenic 5.0%
!!! TTATATTAATTTAAAATACA+AGG - chr4.3:63168640-63168659 None:intergenic 5.0%
AGCTTTCTCTGCCTCCACAT+AGG - chr4.3:63170085-63170104 None:intergenic 50.0%
CCCCTCACAACCAACACTAT+TGG - chr4.3:63169124-63169143 None:intergenic 50.0%
GTAAGTGCCTACTCACCACT+TGG + chr4.3:63170136-63170155 MS.gene87111:CDS 50.0%
GTGATCACTCCCCCACAATT+TGG - chr4.3:63168888-63168907 None:intergenic 50.0%
TGAGACAACATGGGTAGGGA+TGG + chr4.3:63169567-63169586 MS.gene87111:CDS 50.0%
!! CAAAACCGGTTTCTGAGGTG+AGG - chr4.3:63169204-63169223 None:intergenic 50.0%
!! GCCCAATAGTGTTGGTTGTG+AGG + chr4.3:63169119-63169138 MS.gene87111:intron 50.0%
ACACAAAGCAACCCCAGCAC+AGG + chr4.3:63170228-63170247 MS.gene87111:CDS 55.0%
AGTGCCTACTCACCACTTGG+TGG + chr4.3:63170139-63170158 MS.gene87111:CDS 55.0%
GTTGATCCCCATGCATTCCC+TGG - chr4.3:63170166-63170185 None:intergenic 55.0%
TGCAGTCCACAATGCCCTTG+AGG + chr4.3:63168217-63168236 MS.gene87111:CDS 55.0%
TGGTCCACCAAGTGGTGAGT+AGG - chr4.3:63170146-63170165 None:intergenic 55.0%
! ACTCACCACTTGGTGGACCA+GGG + chr4.3:63170146-63170165 MS.gene87111:CDS 55.0%
! TACTCACCACTTGGTGGACC+AGG + chr4.3:63170145-63170164 MS.gene87111:CDS 55.0%
! TGGTGGACCAGGGAATGCAT+GGG + chr4.3:63170156-63170175 MS.gene87111:CDS 55.0%
! TTGGTGGACCAGGGAATGCA+TGG + chr4.3:63170155-63170174 MS.gene87111:CDS 55.0%
GCATTCCCTGGTCCACCAAG+TGG - chr4.3:63170154-63170173 None:intergenic 60.0%
GGTGGACCAGGGAATGCATG+GGG + chr4.3:63170157-63170176 MS.gene87111:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 63168119 63170786 63168119 ID=MS.gene87111
chr4.3 mRNA 63168119 63170786 63168119 ID=MS.gene87111.t1;Parent=MS.gene87111
chr4.3 exon 63168119 63168238 63168119 ID=MS.gene87111.t1.exon1;Parent=MS.gene87111.t1
chr4.3 CDS 63168119 63168238 63168119 ID=cds.MS.gene87111.t1;Parent=MS.gene87111.t1
chr4.3 exon 63168767 63168942 63168767 ID=MS.gene87111.t1.exon2;Parent=MS.gene87111.t1
chr4.3 CDS 63168767 63168942 63168767 ID=cds.MS.gene87111.t1;Parent=MS.gene87111.t1
chr4.3 exon 63169456 63169699 63169456 ID=MS.gene87111.t1.exon3;Parent=MS.gene87111.t1
chr4.3 CDS 63169456 63169699 63169456 ID=cds.MS.gene87111.t1;Parent=MS.gene87111.t1
chr4.3 exon 63170070 63170249 63170070 ID=MS.gene87111.t1.exon4;Parent=MS.gene87111.t1
chr4.3 CDS 63170070 63170249 63170070 ID=cds.MS.gene87111.t1;Parent=MS.gene87111.t1
chr4.3 exon 63170559 63170786 63170559 ID=MS.gene87111.t1.exon5;Parent=MS.gene87111.t1
chr4.3 CDS 63170559 63170786 63170559 ID=cds.MS.gene87111.t1;Parent=MS.gene87111.t1
Gene Sequence

>MS.gene87111

ATGAGGTGCAATCATGTGCGTTTGAATTGTGGCATTACAATGCCTCTCATTGGATTTGGCACTTATTCCTACCCAAATGATAAGAAGACAACAGAAAATGCAGTCCACAATGCCCTTGAGGTACTAACCTTAATCACATACACATTTGTTGTATGAAGATCATATTGTTGTTATAGATTGTTATATGAATATCAAATAACGAGTAAACCATTAAACTGGTCTCTATCTTTGTAAGTTACTTTTAAATTAGTTCCTGCCGCTATTAATATTTTAAACTAGTCTTCATCTTTGTTAAAAGTTTCTCAATTAAGTTCCTCGTCAAATGACAAAAACATGACGGAGACCTAATTGAGAAACTTTTGGCAAAAAAAGGGACTAGTTTAAAATATTAACACAGTCAGAGACTAATTTGATAATGACTTACAAAAGTCAAAAACAGAGATTAATTTGATGGTTTACTCATCAAATAACTTGTATATTGAAATGTATTTTAAAACTGATATGAAATTAAACTCCTCCCTTGTATTTTAAATTAATATAAGTAAAATATGTGCATGTGTTTAGTCTAACTATATAGAAAATTTTCTTGCTATTGTTCTTATCTTTCCTTATTATTGAACACAAATATATATGTGTGTATGTATGCAGGTGGGTTATAGACATTTTGACACGGCCAAAATTTATGGTTCTGAGCCAGCATTAGGCAATGCCATAAATAAGGCAATCTACATGGGAGAAGTAAAGAGGGAAGATATTTTCTTGACATCCAAATTGTGGGGGAGTGATCACCATGATCCTGTTGCTGCATTAAAACAAACTCTAAAGTAAGTGAATAGAAAATAAACAAAATTCATATGAATTGCATGCTATATCTTAATCCTTTTCATTTCATGTAAAAAAAAATTCTAAGATGGTATGAGAGTCACCGCTAAGATCTATTGGATCACATGCTATCAGGTTTTCACTATCGGGTTACCCACCATTTATGTCCATGAACCAAGCCCAATAGTGTTGGTTGTGAGGGGGATGTTAAAGAGTCTCGCATCAGATAGCATGAACATGTATTTATAAGTGGGGACAATCCTCACCTCAGAAACCGGTTTTGAGAGATTGAGTTAGGTCTGACCACGAAATTTAAGAATAATTTAAAGTATAGAACATGTGACACAATAAGTAATGCACAGAATGTGTCTAACACCAAATTGCAACAACCAATGGTTTGTCACTACCAAAATTAAAATCTGATGCCTATTGTGTGACAAATCTTTTGAATTACTTAAAAAGTTTTATTTGAGATTTAATATTGATTTTTGGTTTTTACTTGAATGCAAAAACAGGAATCTGGACATGGACTATTTGGATATGTACCTAGTGCATTGGCCTGTAAAGTTAAAACCATGGGTTAACTATCCTGTTCCTAATGAAGATGACTTTGAAAATTTGGATCTTGAGACAACATGGGTAGGGATGGAAAAATGTCTTGAAATGGGGTTATGTAGGTCCATTGGAGTTAGTAATTTCTCTAGCAACAAGATTGAGTGGCTCTTAGATTTTGCTAGTACACCTCCAGTTGTTAATCAGGTAATTAACTAATTGGAATTTATCTTGAGTAGCACTTGTATCACATCACTATTATAAGAAAAAACCATTTGTAGGTTGATCTAATAATTAATCTATTTGATCTATAATATAATTCACACACATCAATTATTGATTGAAATTAAAAAGTTGTTTTTTGTTTACTGAATGAAGTAACCCATTTGGGATAATCCGATGGTGTTTGCTTAAGACTTTAGAGTGTGCTCATCTCAAGATTTTAAATTTCATTCTCCCTAATGTCAATATCAGTAGACTGGTTTGACTTCTTGAAAAAATGAGTAAATGAAGTGGTTTTATCATTGACAATACGAATAGAATAATACAACAATTTAATATCTTGATATATACTTAGGTGGAAATGCATCCTATGTGGAGGCAGAGAAAGCTGAGAAAGACATGTGGAGAGCACAAGATACATGTAAGTGCCTACTCACCACTTGGTGGACCAGGGAATGCATGGGGATCAACAGCTGTTGTTAATCATCCAATTATACAATCAATTGCCTTCAAACACAAAGCAACCCCAGCACAGGTAATTAAATTTCAAGTTTAAGACTTTTTTTAGAAAAGATTTTTGTAATCTAGACTTAAGTAAATAAAATATATTCAAAAATTGTGAATATTGTTTTAATCAAAATTCAAACTTGTCTATTCTTGATAGTACATAACATAATAAATAAAAATAATTACTCCAATGTTCAAATAAATTTGACACATTATTTAAATGTACAATGATATAAAATTGATATCCAAATTAGTTGCATTTGTATCATTAGTAAAATGAATGGTAATGTATAAATAATTTAATAAGCTAATTTTGACTATGATCTTTGATTTGAAGGTTGCATTGAAATGGGGATTATCAAAGGGCTCAAGTGTGATTGTGAAAAGTTTCAATGATGAAAGAATGGAGGAAAATATGGGATCATTTGATCTAAACTTGGATGATGAAGATATATTTGAGATTGAAAAATTGGAAGAAATGAAGATTATGAGAGGGGAATTTCATGTTAACCAAACCACAAGTCCCTACAAAACAATTGAAGAACTTTGGGATGATGAAATTTGA
Protein sequence

>MS.gene87111.t1

MRCNHVRLNCGITMPLIGFGTYSYPNDKKTTENAVHNALEVGYRHFDTAKIYGSEPALGNAINKAIYMGEVKREDIFLTSKLWGSDHHDPVAALKQTLKNLDMDYLDMYLVHWPVKLKPWVNYPVPNEDDFENLDLETTWVGMEKCLEMGLCRSIGVSNFSSNKIEWLLDFASTPPVVNQVEMHPMWRQRKLRKTCGEHKIHVSAYSPLGGPGNAWGSTAVVNHPIIQSIAFKHKATPAQVALKWGLSKGSSVIVKSFNDERMEENMGSFDLNLDDEDIFEIEKLEEMKIMRGEFHVNQTTSPYKTIEELWDDEI