AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91446

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91446.t1 MTR_4g036845 97.460 315 8 0 1 315 1 315 0.0 647
MS.gene91446.t1 MTR_4g072320 46.057 317 162 5 2 315 5 315 2.11e-95 285
MS.gene91446.t1 MTR_6g073110 45.981 311 163 4 6 312 9 318 2.49e-90 272
MS.gene91446.t1 MTR_4g072350 46.226 318 164 6 2 314 5 320 2.93e-90 272
MS.gene91446.t1 MTR_8g070095 43.590 312 170 3 8 315 10 319 2.01e-87 265
MS.gene91446.t1 MTR_4g072360 45.283 318 167 6 2 314 5 320 3.98e-87 264
MS.gene91446.t1 MTR_8g070115 43.260 319 170 5 3 315 2 315 4.51e-86 261
MS.gene91446.t1 MTR_0374s0050 43.464 306 158 6 14 314 20 315 1.49e-85 260
MS.gene91446.t1 MTR_7g063580 41.433 321 167 5 4 315 46 354 7.88e-79 244
MS.gene91446.t1 MTR_4g021350 41.801 311 173 4 7 315 9 313 5.58e-76 235
MS.gene91446.t1 MTR_3g083130 39.672 305 170 7 14 314 21 315 2.62e-74 231
MS.gene91446.t1 MTR_4g021410 40.645 310 165 5 8 314 11 304 1.18e-67 214
MS.gene91446.t1 MTR_4g021450 40.323 310 166 5 8 314 11 304 1.91e-66 211
MS.gene91446.t1 MTR_4g021350 43.116 276 148 5 7 280 9 277 3.85e-65 207
MS.gene91446.t1 MTR_5g097910 40.000 305 170 6 12 313 18 312 1.41e-64 206
MS.gene91446.t1 MTR_5g097940 40.000 305 170 6 12 313 18 312 1.41e-64 206
MS.gene91446.t1 MTR_5g097900 40.702 285 164 4 12 293 18 300 3.60e-64 205
MS.gene91446.t1 MTR_4g021410 41.727 278 147 3 39 314 3 267 5.30e-64 203
MS.gene91446.t1 MTR_7g070500 38.413 315 175 8 8 314 17 320 6.67e-61 197
MS.gene91446.t1 MTR_4g072060 37.500 304 172 5 6 299 3 298 6.68e-58 189
MS.gene91446.t1 MTR_7g070500 39.085 284 156 6 8 285 17 289 1.66e-53 177
MS.gene91446.t1 MTR_4g021410 40.664 241 128 3 76 314 1 228 2.83e-49 164
MS.gene91446.t1 MTR_4g021450 40.909 242 126 4 76 314 1 228 3.29e-49 164
MS.gene91446.t1 MTR_4g021450 42.541 181 98 2 135 314 10 185 3.33e-45 152
MS.gene91446.t1 MTR_6g072650 39.456 147 66 2 168 314 13 136 2.36e-28 107
MS.gene91446.t1 MTR_6g072710 44.248 113 63 0 183 295 1 113 3.71e-25 97.8
MS.gene91446.t1 MTR_3g449790 25.460 326 172 12 12 285 45 351 6.64e-17 80.9
MS.gene91446.t1 MTR_3g449790 24.540 326 169 11 12 285 45 345 2.85e-16 79.0
MS.gene91446.t1 MTR_7g114980 26.087 322 173 12 20 298 35 334 4.74e-15 75.1
MS.gene91446.t1 MTR_1g056790 94.118 34 2 0 263 296 1 34 2.30e-14 67.0
MS.gene91446.t1 MTR_7g021850 26.911 327 164 15 11 288 13 313 1.36e-13 70.9
MS.gene91446.t1 MTR_1g102750 24.848 330 180 11 11 294 14 321 5.42e-13 68.9
MS.gene91446.t1 MTR_7g114990 25.667 300 164 10 20 282 37 314 5.70e-13 68.9
MS.gene91446.t1 MTR_7g114980 26.812 276 139 11 20 254 35 288 1.75e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91446.t1 AT5G62420 67.722 316 101 1 1 315 1 316 1.57e-162 455
MS.gene91446.t1 AT2G37770 44.695 311 166 3 7 315 9 315 8.27e-91 273
MS.gene91446.t1 AT2G37790 43.730 311 166 5 8 315 10 314 5.63e-85 258
MS.gene91446.t1 AT1G59960 42.071 309 171 7 13 315 20 326 1.18e-84 258
MS.gene91446.t1 AT2G37760 41.935 310 170 3 8 315 10 311 1.70e-81 249
MS.gene91446.t1 AT2G37760 41.935 310 170 3 8 315 10 311 1.70e-81 249
MS.gene91446.t1 AT3G53880 42.122 311 174 3 7 315 9 315 2.63e-80 246
MS.gene91446.t1 AT1G59950 41.883 308 171 7 14 315 15 320 1.56e-74 232
MS.gene91446.t1 AT2G37760 40.559 286 160 3 8 291 10 287 1.74e-73 228
MS.gene91446.t1 AT2G37760 40.559 286 160 3 8 291 10 287 1.74e-73 228
MS.gene91446.t1 AT2G37760 42.909 275 146 4 8 280 10 275 2.86e-73 228
MS.gene91446.t1 AT5G01670 39.103 312 179 7 7 314 17 321 3.59e-71 223
MS.gene91446.t1 AT2G21250 39.384 292 165 5 6 287 3 292 1.26e-68 216
MS.gene91446.t1 AT2G21260 39.175 291 165 5 6 286 3 291 3.55e-68 215
MS.gene91446.t1 AT3G53880 40.493 284 163 3 7 288 9 288 2.29e-67 213
MS.gene91446.t1 AT5G01670 36.283 339 178 8 7 314 17 348 5.10e-67 214
MS.gene91446.t1 AT2G37760 42.308 260 146 2 58 315 8 265 9.36e-66 207
MS.gene91446.t1 AT2G37760 42.308 260 146 2 58 315 8 265 9.36e-66 207
MS.gene91446.t1 AT2G21250 39.056 233 130 5 6 228 3 233 5.02e-52 171
MS.gene91446.t1 AT2G37770 42.500 200 109 3 7 204 9 204 2.44e-49 166
MS.gene91446.t1 AT2G21260 39.091 220 124 4 78 287 5 224 3.58e-47 159
MS.gene91446.t1 AT1G06690 26.471 306 165 11 29 293 85 371 1.52e-18 85.5
MS.gene91446.t1 AT1G60710 26.126 333 174 13 8 298 24 326 2.71e-13 70.1
MS.gene91446.t1 AT5G53580 24.359 312 177 8 17 283 50 347 3.39e-13 69.7
MS.gene91446.t1 AT1G60750 24.422 303 178 11 22 298 34 311 1.06e-11 65.1
MS.gene91446.t1 AT1G60690 25.145 346 183 15 2 297 6 325 1.71e-11 64.7
MS.gene91446.t1 AT1G60730 25.075 335 183 13 8 302 24 330 2.78e-11 63.9
MS.gene91446.t1 AT1G60730 24.620 329 189 12 22 302 33 350 3.63e-11 63.9

Find 81 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGATGACTTTGAAAATT+TGG 0.094815 4.2:+66741809 MS.gene91446:CDS
TATCTTTGAAATTGAGAAAT+TGG 0.186598 4.2:+66742982 MS.gene91446:CDS
AAGGCAATTGATTGTATAAT+TGG 0.200651 4.2:-66742471 None:intergenic
CATGTAGATTGCCTTATTTA+TGG 0.211532 4.2:-66741094 None:intergenic
GAATCTGGACATGGACTATT+TGG 0.212045 4.2:+66741725 MS.gene91446:CDS
TCTGTTGTCTTCTTATCGTT+TGG 0.296217 4.2:-66740499 None:intergenic
CAAAACAATTGAAGAACTTT+GGG 0.318085 4.2:+66743060 MS.gene91446:CDS
ACAATGCCTCTCATTGGATT+TGG 0.330693 4.2:+66740463 MS.gene91446:CDS
ACAAAACAATTGAAGAACTT+TGG 0.331563 4.2:+66743059 MS.gene91446:CDS
TGATATTTAATTACCTGTGC+TGG 0.338494 4.2:-66742510 None:intergenic
ATTAGGCAATGCCATAAATA+AGG 0.350442 4.2:+66741083 MS.gene91446:CDS
GAAAGAATGGAGGAAAATAT+TGG 0.358352 4.2:+66742929 MS.gene91446:CDS
ATTTGAAGGTTGCATTGAAA+TGG 0.361915 4.2:+66742861 MS.gene91446:intron
CTGTTGTCTTCTTATCGTTT+GGG 0.362633 4.2:-66740498 None:intergenic
TGGGGTTGCTTTGTGTTTGA+AGG 0.388559 4.2:-66742490 None:intergenic
TGAATGCAAAATCAGGAATC+TGG 0.394622 4.2:+66741710 MS.gene91446:intron
GGCATTACAATGCCTCTCAT+TGG 0.395160 4.2:+66740457 MS.gene91446:CDS
TACACCTCCAGTTGTTAATC+AGG 0.396062 4.2:+66741947 MS.gene91446:CDS
GTTTGTTTCAATGCAGCAAC+AGG 0.398249 4.2:-66741180 None:intergenic
GAGCCACTCAATCTTGTTGC+TGG 0.404318 4.2:-66741910 None:intergenic
TTTGAAGGTTGCATTGAAAT+GGG 0.414907 4.2:+66742862 MS.gene91446:intron
GATATTTAATTACCTGTGCT+GGG 0.417731 4.2:-66742509 None:intergenic
TTAATTACCTGATTAACAAC+TGG 0.420192 4.2:-66741954 None:intergenic
TACTCACCACTTGGTGGACC+AGG 0.421010 4.2:+66742414 MS.gene91446:CDS
AAGTCATCTTCATTAGGAAC+AGG 0.433658 4.2:-66741798 None:intergenic
ATGGGGTTGTGTAGGTCAAT+TGG 0.438748 4.2:+66741873 MS.gene91446:CDS
TTTATGGCATTGCCTAATGC+TGG 0.448486 4.2:-66741078 None:intergenic
CATAAATAAGGCAATCTACA+TGG 0.451120 4.2:+66741095 MS.gene91446:CDS
TTGGTGGACCAGGGAATGCA+TGG 0.463271 4.2:+66742424 MS.gene91446:CDS
TATGGTTCTGAGCCAGCATT+AGG 0.464141 4.2:+66741066 MS.gene91446:CDS
TGGATCATTTGATCTAAACT+TGG 0.477437 4.2:+66742949 MS.gene91446:CDS
GTTGATCCCCATGCATTCCC+TGG 0.477901 4.2:-66742432 None:intergenic
TTTCTTGACATCCAAATTGT+GGG 0.481755 4.2:+66741140 MS.gene91446:CDS
GCCTGTAAAGTTAAAACCAT+GGG 0.488120 4.2:+66741767 MS.gene91446:CDS
AAGTTTCAATGAAGAAAGAA+TGG 0.497943 4.2:+66742916 MS.gene91446:CDS
TGGATATGTACCTAGTGCAT+TGG 0.523656 4.2:+66741745 MS.gene91446:CDS
ATTTGGATCTTGAGACAACA+TGG 0.540374 4.2:+66741826 MS.gene91446:CDS
TGTCTTCTTATCGTTTGGGT+AGG 0.545525 4.2:-66740494 None:intergenic
ACTTTACAGGCCAATGCACT+AGG 0.547784 4.2:-66741755 None:intergenic
AATCATGTGCGTTTGAATTG+TGG 0.556437 4.2:+66740436 MS.gene91446:CDS
TCAATGCAGCAACAGGATCA+TGG 0.559666 4.2:-66741173 None:intergenic
GAGGTGTACTAGCAAAATCT+AGG 0.559832 4.2:-66741932 None:intergenic
TGGTGGACCAGGGAATGCAT+GGG 0.560104 4.2:+66742425 MS.gene91446:CDS
TTGAAATGGGGATTATCAAA+GGG 0.560330 4.2:+66742875 MS.gene91446:CDS
ATTGAAATGGGGATTATCAA+AGG 0.560489 4.2:+66742874 MS.gene91446:CDS
AAGAAATGAAGATTATGAGA+GGG 0.564196 4.2:+66743005 MS.gene91446:CDS
TGAGACAACATGGGTAGGGA+TGG 0.572572 4.2:+66741836 MS.gene91446:CDS
TTCTTGACATCCAAATTGTG+GGG 0.578334 4.2:+66741141 MS.gene91446:CDS
GGAACAGGATAGTTAACCCA+TGG 0.578853 4.2:-66741783 None:intergenic
TGGTCCACCAAGTGGTGAGT+AGG 0.583500 4.2:-66742412 None:intergenic
GATCTTGAGACAACATGGGT+AGG 0.585515 4.2:+66741831 MS.gene91446:CDS
ACACAAAGCAACCCCAGCAC+AGG 0.586444 4.2:+66742497 MS.gene91446:CDS
CTACATGGGAGAAGTAGAGA+GGG 0.588657 4.2:+66741110 MS.gene91446:CDS
TTGAAGGTTGCATTGAAATG+GGG 0.588735 4.2:+66742863 MS.gene91446:intron
GATTAAGGTTTGTACCTCAA+GGG 0.591270 4.2:-66740539 None:intergenic
GAAGAAATGAAGATTATGAG+AGG 0.591725 4.2:+66743004 MS.gene91446:CDS
ATCTTGAGACAACATGGGTA+GGG 0.592828 4.2:+66741832 MS.gene91446:CDS
ATAAATAAGGCAATCTACAT+GGG 0.593397 4.2:+66741096 MS.gene91446:CDS
TGCAGTCCACAATGCCCTTG+AGG 0.593505 4.2:+66740525 MS.gene91446:CDS
CAAAATCAGGAATCTGGACA+TGG 0.599053 4.2:+66741716 MS.gene91446:intron
GGCCTGTAAAGTTAAAACCA+TGG 0.600838 4.2:+66741766 MS.gene91446:CDS
ATATTTAATTACCTGTGCTG+GGG 0.601478 4.2:-66742508 None:intergenic
GTCTTGAAATGGGGTTGTGT+AGG 0.601608 4.2:+66741865 MS.gene91446:CDS
AGAAATGAAGATTATGAGAG+GGG 0.602586 4.2:+66743006 MS.gene91446:CDS
TTTCAATGAAGAAAGAATGG+AGG 0.603687 4.2:+66742919 MS.gene91446:CDS
AGAAAGCTGAGAAAGAGATG+TGG 0.608641 4.2:+66742366 MS.gene91446:CDS
TCTCCAGCAACAAGATTGAG+TGG 0.619388 4.2:+66741907 MS.gene91446:CDS
GTAAGTGCCTACTCACCACT+TGG 0.619442 4.2:+66742405 MS.gene91446:CDS
AGTGCCTACTCACCACTTGG+TGG 0.619529 4.2:+66742408 MS.gene91446:CDS
AGCTTTCTCTGCCTCCACAT+AGG 0.620551 4.2:-66742351 None:intergenic
TCTTGACATCCAAATTGTGG+GGG 0.621026 4.2:+66741142 MS.gene91446:CDS
AGGTGGAAATGCATCCTATG+TGG 0.626109 4.2:+66742337 MS.gene91446:intron
ATTACCTGATTAACAACTGG+AGG 0.626966 4.2:-66741951 None:intergenic
TTTGTACCTCAAGGGCATTG+TGG 0.647390 4.2:-66740531 None:intergenic
TCTACATGGGAGAAGTAGAG+AGG 0.648747 4.2:+66741109 MS.gene91446:CDS
TAAGTGCCAAATCCAATGAG+AGG 0.655261 4.2:-66740469 None:intergenic
TGGAAATGCATCCTATGTGG+AGG 0.657202 4.2:+66742340 MS.gene91446:CDS
GGTGGACCAGGGAATGCATG+GGG 0.659494 4.2:+66742426 MS.gene91446:CDS
GCATTCCCTGGTCCACCAAG+TGG 0.660549 4.2:-66742420 None:intergenic
TTTGGATCTTGAGACAACAT+GGG 0.665864 4.2:+66741827 MS.gene91446:CDS
ACTCACCACTTGGTGGACCA+GGG 0.670084 4.2:+66742415 MS.gene91446:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTAATTTAAGATACAA+GGG - chr4.2:66740908-66740927 None:intergenic 10.0%
!! AAATTGTTGTATTATTCTAT+TGG - chr4.2:66742298-66742317 None:intergenic 15.0%
!! ATATATTTGTGTTGAATAAT+AGG - chr4.2:66740997-66741016 None:intergenic 15.0%
!! TAAATTATTCTTAAATTTCG+TGG - chr4.2:66741512-66741531 None:intergenic 15.0%
!! TAATATCTTGATATATACTT+AGG + chr4.2:66742317-66742336 MS.gene91446:intron 15.0%
!! TATATTTGTGTTGAATAATA+GGG - chr4.2:66740996-66741015 None:intergenic 15.0%
!! TATCAAATTTATTTGAACAT+TGG - chr4.2:66742691-66742710 None:intergenic 15.0%
!! TTAAATTAATATAGTAGAGT+AGG + chr4.2:66740915-66740934 MS.gene91446:intron 15.0%
!!! ATGTAAAAAAAATTCTAAGA+TGG + chr4.2:66741276-66741295 MS.gene91446:intron 15.0%
!!! CTATATTAATTTAAGATACA+AGG - chr4.2:66740909-66740928 None:intergenic 15.0%
!!! TATTTTATTTACTTGTTTGA+TGG - chr4.2:66742580-66742599 None:intergenic 15.0%
!!! TTTTTTTACATGAAATGAAA+AGG - chr4.2:66741267-66741286 None:intergenic 15.0%
!! AATGAAAAGGATTAAGATAT+AGG - chr4.2:66741254-66741273 None:intergenic 20.0%
!! ATGAACATGTATTTATAAGT+GGG + chr4.2:66741437-66741456 MS.gene91446:intron 20.0%
!! TAATCAGGTAATTAACTAAT+TGG + chr4.2:66741962-66741981 MS.gene91446:intron 20.0%
!! TATAAGAAAAAACCATTTGT+AGG + chr4.2:66742021-66742040 MS.gene91446:intron 20.0%
!! TGTATCATTAGTAAAATGAA+TGG + chr4.2:66742762-66742781 MS.gene91446:intron 20.0%
!! TTTGATACAGCAAAAATTTA+TGG + chr4.2:66741048-66741067 MS.gene91446:CDS 20.0%
!!! AAATTAGCTTTTTTTTGAAC+AGG - chr4.2:66742826-66742845 None:intergenic 20.0%
!!! CAAGTTTAAGACTTTTTTTT+TGG + chr4.2:66742531-66742550 MS.gene91446:intron 20.0%
!!! TATCTTTGAAATTGAGAAAT+TGG + chr4.2:66742982-66743001 MS.gene91446:CDS 20.0%
! AAAGACAATACCAATTTGAT+GGG - chr4.2:66740636-66740655 None:intergenic 25.0%
! AACAAATGTGTATGTGATTA+AGG - chr4.2:66740557-66740576 None:intergenic 25.0%
! AAGAAATGAAGATTATGAGA+GGG + chr4.2:66743005-66743024 MS.gene91446:CDS 25.0%
! AAGGCAATTGATTGTATAAT+TGG - chr4.2:66742474-66742493 None:intergenic 25.0%
! AAGTTTCAATGAAGAAAGAA+TGG + chr4.2:66742916-66742935 MS.gene91446:CDS 25.0%
! ACAAAACAATTGAAGAACTT+TGG + chr4.2:66743059-66743078 MS.gene91446:CDS 25.0%
! ATAAATAAGGCAATCTACAT+GGG + chr4.2:66741096-66741115 MS.gene91446:CDS 25.0%
! ATGGAAAAATGTCTTGAAAT+GGG + chr4.2:66741855-66741874 MS.gene91446:CDS 25.0%
! CAAAACAATTGAAGAACTTT+GGG + chr4.2:66743060-66743079 MS.gene91446:CDS 25.0%
! CATGAACATGTATTTATAAG+TGG + chr4.2:66741436-66741455 MS.gene91446:intron 25.0%
! GAAAAAATGAGTTAATGAAG+TGG + chr4.2:66742255-66742274 MS.gene91446:intron 25.0%
! TATCTTGATATATACTTAGG+TGG + chr4.2:66742320-66742339 MS.gene91446:intron 25.0%
! TGAACATGTATTTATAAGTG+GGG + chr4.2:66741438-66741457 MS.gene91446:intron 25.0%
! TGATACAAATGCAACTAATT+TGG - chr4.2:66742749-66742768 None:intergenic 25.0%
! TTAATTACCTGATTAACAAC+TGG - chr4.2:66741957-66741976 None:intergenic 25.0%
! TTATTAGATCAACCTACAAA+TGG - chr4.2:66742036-66742055 None:intergenic 25.0%
! TTGTCAAAAGTTTCTCAATT+AGG - chr4.2:66740741-66740760 None:intergenic 25.0%
!! AAAAGATTTTGTCACACAAT+AGG - chr4.2:66741633-66741652 None:intergenic 25.0%
!! TGAAGATGACTTTGAAAATT+TGG + chr4.2:66741809-66741828 MS.gene91446:CDS 25.0%
!! TTTTACTTGAATGCAAAATC+AGG + chr4.2:66741703-66741722 MS.gene91446:intron 25.0%
!! TTTTCAAAGTCATCTTCATT+AGG - chr4.2:66741807-66741826 None:intergenic 25.0%
!!! AGTTCTTCAATTGTTTTGTA+AGG - chr4.2:66743058-66743077 None:intergenic 25.0%
!!! ATAGGCATCAGATTTTATTT+TGG - chr4.2:66741615-66741634 None:intergenic 25.0%
!!! CTTTTTGTTTTTGTCAAAGA+CGG - chr4.2:66740711-66740730 None:intergenic 25.0%
!!! TGAGATTTAGTACTGATTTT+TGG + chr4.2:66741679-66741698 MS.gene91446:intron 25.0%
AAAGACATGGATCAATTTGA+TGG + chr4.2:66740819-66740838 MS.gene91446:intron 30.0%
AGAAATGAAGATTATGAGAG+GGG + chr4.2:66743006-66743025 MS.gene91446:CDS 30.0%
ATATATGTATGTGTGTATGC+AGG + chr4.2:66741011-66741030 MS.gene91446:intron 30.0%
ATATTTAATTACCTGTGCTG+GGG - chr4.2:66742511-66742530 None:intergenic 30.0%
ATTAGGCAATGCCATAAATA+AGG + chr4.2:66741083-66741102 MS.gene91446:CDS 30.0%
ATTGAAATGGGGATTATCAA+AGG + chr4.2:66742874-66742893 MS.gene91446:CDS 30.0%
ATTTGAAGGTTGCATTGAAA+TGG + chr4.2:66742861-66742880 MS.gene91446:intron 30.0%
CAAAGACAATACCAATTTGA+TGG - chr4.2:66740637-66740656 None:intergenic 30.0%
CATAAATAAGGCAATCTACA+TGG + chr4.2:66741095-66741114 MS.gene91446:CDS 30.0%
GAAAGAATGGAGGAAAATAT+TGG + chr4.2:66742929-66742948 MS.gene91446:CDS 30.0%
GAAGAAATGAAGATTATGAG+AGG + chr4.2:66743004-66743023 MS.gene91446:CDS 30.0%
GAGAATTAGTTACAAAGACA+TGG + chr4.2:66740806-66740825 MS.gene91446:intron 30.0%
GATATTTAATTACCTGTGCT+GGG - chr4.2:66742512-66742531 None:intergenic 30.0%
GATGGAAAAATGTCTTGAAA+TGG + chr4.2:66741854-66741873 MS.gene91446:CDS 30.0%
TGACAAAAACAAAAAGACAG+CGG + chr4.2:66740715-66740734 MS.gene91446:intron 30.0%
TGATATTTAATTACCTGTGC+TGG - chr4.2:66742513-66742532 None:intergenic 30.0%
TGGAAAAATGTCTTGAAATG+GGG + chr4.2:66741856-66741875 MS.gene91446:CDS 30.0%
TGGATCATTTGATCTAAACT+TGG + chr4.2:66742949-66742968 MS.gene91446:CDS 30.0%
TTGAAATGGGGATTATCAAA+GGG + chr4.2:66742875-66742894 MS.gene91446:CDS 30.0%
TTTCAATGAAGAAAGAATGG+AGG + chr4.2:66742919-66742938 MS.gene91446:CDS 30.0%
TTTCTTGACATCCAAATTGT+GGG + chr4.2:66741140-66741159 MS.gene91446:CDS 30.0%
TTTGAAGGTTGCATTGAAAT+GGG + chr4.2:66742862-66742881 MS.gene91446:intron 30.0%
! TTTTCTTGACATCCAAATTG+TGG + chr4.2:66741139-66741158 MS.gene91446:CDS 30.0%
!! AATTGTTTTGTAAGGACTTG+TGG - chr4.2:66743050-66743069 None:intergenic 30.0%
!! CATGTAGATTGCCTTATTTA+TGG - chr4.2:66741097-66741116 None:intergenic 30.0%
!! GACTATGATCTTTGATTTGA+AGG + chr4.2:66742847-66742866 MS.gene91446:intron 30.0%
AAGTCATCTTCATTAGGAAC+AGG - chr4.2:66741801-66741820 None:intergenic 35.0%
AAGTCTTAAGCAAACACCAT+CGG - chr4.2:66742161-66742180 None:intergenic 35.0%
AATCATGTGCGTTTGAATTG+TGG + chr4.2:66740436-66740455 MS.gene91446:CDS 35.0%
ACTGAATGAAGTAACCCATT+TGG + chr4.2:66742129-66742148 MS.gene91446:intron 35.0%
ACTTGGTTCATGGACATAAA+AGG - chr4.2:66741367-66741386 None:intergenic 35.0%
AGTCCACTGATATTGACATT+AGG - chr4.2:66742222-66742241 None:intergenic 35.0%
ATTACCTGATTAACAACTGG+AGG - chr4.2:66741954-66741973 None:intergenic 35.0%
ATTTGGATCTTGAGACAACA+TGG + chr4.2:66741826-66741845 MS.gene91446:CDS 35.0%
CTAACTCAATCTCTCAAAAC+TGG - chr4.2:66741483-66741502 None:intergenic 35.0%
CTGAATGAAGTAACCCATTT+GGG + chr4.2:66742130-66742149 MS.gene91446:intron 35.0%
CTGTTGTCTTCTTATCGTTT+GGG - chr4.2:66740501-66740520 None:intergenic 35.0%
GATTAAGGTTTGTACCTCAA+GGG - chr4.2:66740542-66740561 None:intergenic 35.0%
GCCTGTAAAGTTAAAACCAT+GGG + chr4.2:66741767-66741786 MS.gene91446:CDS 35.0%
GTCCACTGATATTGACATTA+GGG - chr4.2:66742221-66742240 None:intergenic 35.0%
TAATGTCAATATCAGTGGAC+TGG + chr4.2:66742221-66742240 MS.gene91446:intron 35.0%
TCTGTTGTCTTCTTATCGTT+TGG - chr4.2:66740502-66740521 None:intergenic 35.0%
TGAATGCAAAATCAGGAATC+TGG + chr4.2:66741710-66741729 MS.gene91446:intron 35.0%
TGATTAAGGTTTGTACCTCA+AGG - chr4.2:66740543-66740562 None:intergenic 35.0%
TGTGATCCAATGAATCTTAG+TGG - chr4.2:66741313-66741332 None:intergenic 35.0%
TTCTTGACATCCAAATTGTG+GGG + chr4.2:66741141-66741160 MS.gene91446:CDS 35.0%
TTGAAGGTTGCATTGAAATG+GGG + chr4.2:66742863-66742882 MS.gene91446:intron 35.0%
TTTGGATCTTGAGACAACAT+GGG + chr4.2:66741827-66741846 MS.gene91446:CDS 35.0%
! AACCATTGGTTGTTGCAATT+TGG - chr4.2:66741584-66741603 None:intergenic 35.0%
! TGCTATCAGGTTTTCACTAT+CGG + chr4.2:66741333-66741352 MS.gene91446:intron 35.0%
!!! ACCCATGGTTTTAACTTTAC+AGG - chr4.2:66741771-66741790 None:intergenic 35.0%
!!! CAGTTTTGAGAGATTGAGTT+AGG + chr4.2:66741481-66741500 MS.gene91446:intron 35.0%
!!! GGCATCAGATTTTATTTTGG+TGG - chr4.2:66741612-66741631 None:intergenic 35.0%
ACAATGCCTCTCATTGGATT+TGG + chr4.2:66740463-66740482 MS.gene91446:CDS 40.0%
ACCATCGGATTATCCCAAAT+GGG - chr4.2:66742146-66742165 None:intergenic 40.0%
AGAAAGCTGAGAAAGAGATG+TGG + chr4.2:66742366-66742385 MS.gene91446:CDS 40.0%
ATCTTGAGACAACATGGGTA+GGG + chr4.2:66741832-66741851 MS.gene91446:CDS 40.0%
ATGTATGTGTGTATGCAGGT+GGG + chr4.2:66741015-66741034 MS.gene91446:intron 40.0%
CAAAATCAGGAATCTGGACA+TGG + chr4.2:66741716-66741735 MS.gene91446:intron 40.0%
CACCAAATTGCAACAACCAA+TGG + chr4.2:66741579-66741598 MS.gene91446:intron 40.0%
CTCCCTAATGTCAATATCAG+TGG + chr4.2:66742216-66742235 MS.gene91446:intron 40.0%
GAGGTGTACTAGCAAAATCT+AGG - chr4.2:66741935-66741954 None:intergenic 40.0%
GAGTCTCCACTAAGATTCAT+TGG + chr4.2:66741304-66741323 MS.gene91446:intron 40.0%
GGCCTGTAAAGTTAAAACCA+TGG + chr4.2:66741766-66741785 MS.gene91446:CDS 40.0%
GGTTCATGGACATAAAAGGT+GGG - chr4.2:66741363-66741382 None:intergenic 40.0%
GTTTGTTTCAATGCAGCAAC+AGG - chr4.2:66741183-66741202 None:intergenic 40.0%
TAACGAGCAACCCATCAAAT+TGG + chr4.2:66740623-66740642 MS.gene91446:intron 40.0%
TAAGTGCCAAATCCAATGAG+AGG - chr4.2:66740472-66740491 None:intergenic 40.0%
TACACCTCCAGTTGTTAATC+AGG + chr4.2:66741947-66741966 MS.gene91446:CDS 40.0%
TATGTATGTGTGTATGCAGG+TGG + chr4.2:66741014-66741033 MS.gene91446:intron 40.0%
TCATTGGATCACATGCTATC+AGG + chr4.2:66741320-66741339 MS.gene91446:intron 40.0%
TCTTGACATCCAAATTGTGG+GGG + chr4.2:66741142-66741161 MS.gene91446:CDS 40.0%
TGGATATGTACCTAGTGCAT+TGG + chr4.2:66741745-66741764 MS.gene91446:CDS 40.0%
TGGTTCATGGACATAAAAGG+TGG - chr4.2:66741364-66741383 None:intergenic 40.0%
TGTCTTCTTATCGTTTGGGT+AGG - chr4.2:66740497-66740516 None:intergenic 40.0%
TTTATGGCATTGCCTAATGC+TGG - chr4.2:66741081-66741100 None:intergenic 40.0%
! GAATCTGGACATGGACTATT+TGG + chr4.2:66741725-66741744 MS.gene91446:CDS 40.0%
! GCTATCAGGTTTTCACTATC+GGG + chr4.2:66741334-66741353 MS.gene91446:intron 40.0%
!!! TTTTGGTGGTGACAAACCAT+TGG - chr4.2:66741598-66741617 None:intergenic 40.0%
ACAACCAGCACTATTGGACT+TGG - chr4.2:66741384-66741403 None:intergenic 45.0%
ACTTTACAGGCCAATGCACT+AGG - chr4.2:66741758-66741777 None:intergenic 45.0%
AGGTGGAAATGCATCCTATG+TGG + chr4.2:66742337-66742356 MS.gene91446:intron 45.0%
ATGGGGTTGTGTAGGTCAAT+TGG + chr4.2:66741873-66741892 MS.gene91446:CDS 45.0%
CACCATCGGATTATCCCAAA+TGG - chr4.2:66742147-66742166 None:intergenic 45.0%
CTACATGGGAGAAGTAGAGA+GGG + chr4.2:66741110-66741129 MS.gene91446:CDS 45.0%
GATCTTGAGACAACATGGGT+AGG + chr4.2:66741831-66741850 MS.gene91446:CDS 45.0%
GGAACAGGATAGTTAACCCA+TGG - chr4.2:66741786-66741805 None:intergenic 45.0%
GGCATTACAATGCCTCTCAT+TGG + chr4.2:66740457-66740476 MS.gene91446:CDS 45.0%
GTCTTGAAATGGGGTTGTGT+AGG + chr4.2:66741865-66741884 MS.gene91446:CDS 45.0%
TCAATGCAGCAACAGGATCA+TGG - chr4.2:66741176-66741195 None:intergenic 45.0%
TCTACATGGGAGAAGTAGAG+AGG + chr4.2:66741109-66741128 MS.gene91446:CDS 45.0%
TCTCCAGCAACAAGATTGAG+TGG + chr4.2:66741907-66741926 MS.gene91446:CDS 45.0%
TGAACCAAGTCCAATAGTGC+TGG + chr4.2:66741377-66741396 MS.gene91446:intron 45.0%
TGGAAATGCATCCTATGTGG+AGG + chr4.2:66742340-66742359 MS.gene91446:CDS 45.0%
! ACCCATTTGGGATAATCCGA+TGG + chr4.2:66742142-66742161 MS.gene91446:intron 45.0%
! GCACTATTGGACTTGGTTCA+TGG - chr4.2:66741377-66741396 None:intergenic 45.0%
! TCTCTCAAAACTGGCTTCTG+AGG - chr4.2:66741474-66741493 None:intergenic 45.0%
! TGGGGTTGCTTTGTGTTTGA+AGG - chr4.2:66742493-66742512 None:intergenic 45.0%
!! TATGGTTCTGAGCCAGCATT+AGG + chr4.2:66741066-66741085 MS.gene91446:CDS 45.0%
!! TCCAATAGTGCTGGTTGTGA+GGG + chr4.2:66741386-66741405 MS.gene91446:intron 45.0%
!! TTTGTACCTCAAGGGCATTG+TGG - chr4.2:66740534-66740553 None:intergenic 45.0%
AGCTTTCTCTGCCTCCACAT+AGG - chr4.2:66742354-66742373 None:intergenic 50.0%
GAGCCACTCAATCTTGTTGC+TGG - chr4.2:66741913-66741932 None:intergenic 50.0%
GTAAGTGCCTACTCACCACT+TGG + chr4.2:66742405-66742424 MS.gene91446:CDS 50.0%
GTGATCACTCCCCCACAATT+TGG - chr4.2:66741154-66741173 None:intergenic 50.0%
TGAGACAACATGGGTAGGGA+TGG + chr4.2:66741836-66741855 MS.gene91446:CDS 50.0%
!! CAAAACTGGCTTCTGAGGTG+AGG - chr4.2:66741469-66741488 None:intergenic 50.0%
!! CCAATAGTGCTGGTTGTGAG+GGG + chr4.2:66741387-66741406 MS.gene91446:intron 50.0%
!! GTCCAATAGTGCTGGTTGTG+AGG + chr4.2:66741385-66741404 MS.gene91446:intron 50.0%
ACACAAAGCAACCCCAGCAC+AGG + chr4.2:66742497-66742516 MS.gene91446:CDS 55.0%
AGTGCCTACTCACCACTTGG+TGG + chr4.2:66742408-66742427 MS.gene91446:CDS 55.0%
CCCCTCACAACCAGCACTAT+TGG - chr4.2:66741390-66741409 None:intergenic 55.0%
GTTGATCCCCATGCATTCCC+TGG - chr4.2:66742435-66742454 None:intergenic 55.0%
TGCAGTCCACAATGCCCTTG+AGG + chr4.2:66740525-66740544 MS.gene91446:CDS 55.0%
TGGTCCACCAAGTGGTGAGT+AGG - chr4.2:66742415-66742434 None:intergenic 55.0%
! ACTCACCACTTGGTGGACCA+GGG + chr4.2:66742415-66742434 MS.gene91446:CDS 55.0%
! TACTCACCACTTGGTGGACC+AGG + chr4.2:66742414-66742433 MS.gene91446:CDS 55.0%
! TGGTGGACCAGGGAATGCAT+GGG + chr4.2:66742425-66742444 MS.gene91446:CDS 55.0%
! TTGGTGGACCAGGGAATGCA+TGG + chr4.2:66742424-66742443 MS.gene91446:CDS 55.0%
GCATTCCCTGGTCCACCAAG+TGG - chr4.2:66742423-66742442 None:intergenic 60.0%
GGTGGACCAGGGAATGCATG+GGG + chr4.2:66742426-66742445 MS.gene91446:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 66740427 66743096 66740427 ID=MS.gene91446
chr4.2 mRNA 66740427 66743096 66740427 ID=MS.gene91446.t1;Parent=MS.gene91446
chr4.2 exon 66740427 66740546 66740427 ID=MS.gene91446.t1.exon1;Parent=MS.gene91446.t1
chr4.2 CDS 66740427 66740546 66740427 ID=cds.MS.gene91446.t1;Parent=MS.gene91446.t1
chr4.2 exon 66741033 66741208 66741033 ID=MS.gene91446.t1.exon2;Parent=MS.gene91446.t1
chr4.2 CDS 66741033 66741208 66741033 ID=cds.MS.gene91446.t1;Parent=MS.gene91446.t1
chr4.2 exon 66741725 66741968 66741725 ID=MS.gene91446.t1.exon3;Parent=MS.gene91446.t1
chr4.2 CDS 66741725 66741968 66741725 ID=cds.MS.gene91446.t1;Parent=MS.gene91446.t1
chr4.2 exon 66742339 66742518 66742339 ID=MS.gene91446.t1.exon4;Parent=MS.gene91446.t1
chr4.2 CDS 66742339 66742518 66742339 ID=cds.MS.gene91446.t1;Parent=MS.gene91446.t1
chr4.2 exon 66742869 66743096 66742869 ID=MS.gene91446.t1.exon5;Parent=MS.gene91446.t1
chr4.2 CDS 66742869 66743096 66742869 ID=cds.MS.gene91446.t1;Parent=MS.gene91446.t1
Gene Sequence

>MS.gene91446

ATGAGGTGCAATCATGTGCGTTTGAATTGTGGCATTACAATGCCTCTCATTGGATTTGGCACTTATTCCTACCCAAACGATAAGAAGACAACAGAAATTGCAGTCCACAATGCCCTTGAGGTACAAACCTTAATCACATACACATTTGTTGTATGAAGATCATATTGTTGTTATAATTGTTATATGAAACATCAAATAACGAGCAACCCATCAAATTGGTATTGTCTTTGTAAGTCGTTCTTAAACTAGTCTATGATTTTATTAATATTTCAAACTAGTCTCCGTCTTTGACAAAAACAAAAAGACAGCGGCCTAATTGAGAAACTTTTGACAAAAACAGAGATTAATTTGAAATATTAATAAAGTCAGTGACTAATTTGAGAATTAGTTACAAAGACATGGATCAATTTGATGGCTTACTCATCAAATAACTCGTATATTGAAATATATTTTAAAACTGATATGAAATTAAAGTACTCCCTTGTATCTTAAATTAATATAGTAGAGTAGGTGCATGTGTTTAGTCTAACTATATTGAAAATTTTCTTGCTATTGTTCTTATCTTTCCCTATTATTCAACACAAATATATGTATGTGTGTATGCAGGTGGGTTATAGACATTTTGATACAGCAAAAATTTATGGTTCTGAGCCAGCATTAGGCAATGCCATAAATAAGGCAATCTACATGGGAGAAGTAGAGAGGGAAGATATTTTCTTGACATCCAAATTGTGGGGGAGTGATCACCATGATCCTGTTGCTGCATTGAAACAAACTCTAAAGTAAGCAAATAGAAAATAAACAAAATGCGTATTAATTGCATGCCTATATCTTAATCCTTTTCATTTCATGTAAAAAAAATTCTAAGATGGTATGAGAGTCTCCACTAAGATTCATTGGATCACATGCTATCAGGTTTTCACTATCGGGTCACCCACCTTTTATGTCCATGAACCAAGTCCAATAGTGCTGGTTGTGAGGGGATGTTAAAGAGTCTCGCATCAGATAGCATGAACATGTATTTATAAGTGGGGACAATCCTCACCTCAGAAGCCAGTTTTGAGAGATTGAGTTAGGTCTGACCACGAAATTTAAGAATAATTTAAAGTATAGAACATGTGACACAATAAGTAATGCACAGAATGTGTCTAACACCAAATTGCAACAACCAATGGTTTGTCACCACCAAAATAAAATCTGATGCCTATTGTGTGACAAAATCTTTTGAATTACTTAAAAAATCAGTTTTATTTGAGATTTAGTACTGATTTTTGGTTTTTACTTGAATGCAAAATCAGGAATCTGGACATGGACTATTTGGATATGTACCTAGTGCATTGGCCTGTAAAGTTAAAACCATGGGTTAACTATCCTGTTCCTAATGAAGATGACTTTGAAAATTTGGATCTTGAGACAACATGGGTAGGGATGGAAAAATGTCTTGAAATGGGGTTGTGTAGGTCAATTGGAGTTAGTAATTTCTCCAGCAACAAGATTGAGTGGCTCCTAGATTTTGCTAGTACACCTCCAGTTGTTAATCAGGTAATTAACTAATTGGAATTTATCTTGAGTAGCACTTGTATCACATCACTATTATAAGAAAAAACCATTTGTAGGTTGATCTAATAATTAATCTATTTGATCTATAATATAATTCACACACATCAATTATTGATTGAAATTAAAAAGTTGTTTTTTGTTTACTGAATGAAGTAACCCATTTGGGATAATCCGATGGTGTTTGCTTAAGACTTTAGAGTGTGCTCATCTCAAGATTTTAAATTTGATTCTCCCTAATGTCAATATCAGTGGACTGGTTTGACTTCTTGAAAAAATGAGTTAATGAAGTGGTTTTATCATTGACAATACCAATAGAATAATACAACAATTTAATATCTTGATATATACTTAGGTGGAAATGCATCCTATGTGGAGGCAGAGAAAGCTGAGAAAGAGATGTGGAGAGCACAAGATACATGTAAGTGCCTACTCACCACTTGGTGGACCAGGGAATGCATGGGGATCAACAGCTGTTGTTAATCATCCAATTATACAATCAATTGCCTTCAAACACAAAGCAACCCCAGCACAGGTAATTAAATATCAAGTTTAAGACTTTTTTTTTGGAAAGATTTTTGTAATCTAGAGTTCCATCAAACAAGTAAATAAAATATATTCAAAAATTGTGAAAATTGTTTTAATCAAAATTCAAACTTGTCTATTCTTGATAGTACATAACATAATAAATAAAAATAATTACTCCAATGTTCAAATAAATTTGATACATTATTTAAATGTACAATGATATAAAATTGATATCCAAATTAGTTGCATTTGTATCATTAGTAAAATGAATGGTAATGTATAAATAATTTAATAAGCTAATTTTGACTATGCCTGTTCAAAAAAAAGCTAATTTTGACTATGATCTTTGATTTGAAGGTTGCATTGAAATGGGGATTATCAAAGGGCTCAAGTGTGATTGTGAAAAGTTTCAATGAAGAAAGAATGGAGGAAAATATTGGATCATTTGATCTAAACTTGGATGATGAAGATATCTTTGAAATTGAGAAATTGGAAGAAATGAAGATTATGAGAGGGGAATTTCATGTTAATGAAACCACAAGTCCTTACAAAACAATTGAAGAACTTTGGGATGATGAAATTTGA
Protein sequence

>MS.gene91446.t1

MRCNHVRLNCGITMPLIGFGTYSYPNDKKTTEIAVHNALEVGYRHFDTAKIYGSEPALGNAINKAIYMGEVEREDIFLTSKLWGSDHHDPVAALKQTLKNLDMDYLDMYLVHWPVKLKPWVNYPVPNEDDFENLDLETTWVGMEKCLEMGLCRSIGVSNFSSNKIEWLLDFASTPPVVNQVEMHPMWRQRKLRKRCGEHKIHVSAYSPLGGPGNAWGSTAVVNHPIIQSIAFKHKATPAQVALKWGLSKGSSVIVKSFNEERMEENIGSFDLNLDDEDIFEIEKLEEMKIMRGEFHVNETTSPYKTIEELWDDEI