AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54933.t1 MTR_4g036845 97.778 315 7 0 1 315 1 315 0.0 648
MS.gene54933.t1 MTR_4g072320 46.057 317 162 5 2 315 5 315 1.75e-95 285
MS.gene54933.t1 MTR_4g072350 45.110 317 169 5 2 314 5 320 3.68e-89 269
MS.gene54933.t1 MTR_6g073110 45.338 311 165 4 6 312 9 318 4.95e-89 269
MS.gene54933.t1 MTR_8g070095 42.628 312 173 3 8 315 10 319 1.25e-86 263
MS.gene54933.t1 MTR_4g072360 44.444 315 170 5 2 312 5 318 3.85e-86 261
MS.gene54933.t1 MTR_0374s0050 44.118 306 156 6 14 314 20 315 1.46e-85 260
MS.gene54933.t1 MTR_8g070115 42.320 319 173 5 3 315 2 315 1.31e-84 258
MS.gene54933.t1 MTR_7g063580 40.498 321 170 5 4 315 46 354 1.78e-77 241
MS.gene54933.t1 MTR_4g021350 41.158 311 175 4 7 315 9 313 6.70e-76 235
MS.gene54933.t1 MTR_3g083130 40.000 305 169 7 14 314 21 315 1.89e-75 234
MS.gene54933.t1 MTR_4g021410 40.323 310 166 5 8 314 11 304 1.80e-68 216
MS.gene54933.t1 MTR_4g021450 39.677 310 168 5 8 314 11 304 5.58e-67 212
MS.gene54933.t1 MTR_4g021350 42.391 276 150 5 7 280 9 277 1.87e-65 207
MS.gene54933.t1 MTR_5g097910 39.672 305 171 6 12 313 18 312 6.63e-65 207
MS.gene54933.t1 MTR_5g097940 39.672 305 171 6 12 313 18 312 6.63e-65 207
MS.gene54933.t1 MTR_5g097900 40.351 285 165 4 12 293 18 300 1.21e-64 206
MS.gene54933.t1 MTR_4g021410 41.367 278 148 3 39 314 3 267 2.99e-64 204
MS.gene54933.t1 MTR_7g070500 38.782 312 178 8 8 314 17 320 1.50e-61 199
MS.gene54933.t1 MTR_4g072060 37.500 304 172 5 6 299 3 298 2.66e-59 192
MS.gene54933.t1 MTR_7g070500 39.502 281 159 6 8 285 17 289 3.86e-54 179
MS.gene54933.t1 MTR_4g021410 40.249 241 129 3 76 314 1 228 1.59e-49 164
MS.gene54933.t1 MTR_4g021450 40.083 242 128 4 76 314 1 228 5.51e-49 163
MS.gene54933.t1 MTR_4g021450 41.989 181 99 2 135 314 10 185 5.11e-45 152
MS.gene54933.t1 MTR_6g072650 38.776 147 67 2 168 314 13 136 1.02e-27 105
MS.gene54933.t1 MTR_6g072710 44.248 113 63 0 183 295 1 113 3.56e-25 97.8
MS.gene54933.t1 MTR_3g449790 25.460 326 172 12 12 285 45 351 8.15e-18 83.2
MS.gene54933.t1 MTR_3g449790 24.540 326 169 11 12 285 45 345 3.55e-17 81.6
MS.gene54933.t1 MTR_7g114980 26.398 322 172 12 20 298 35 334 3.74e-16 78.6
MS.gene54933.t1 MTR_1g056790 94.118 34 2 0 263 296 1 34 2.02e-14 67.0
MS.gene54933.t1 MTR_7g114990 26.000 300 163 10 20 282 37 314 4.55e-14 72.4
MS.gene54933.t1 MTR_1g102750 24.776 335 174 12 11 294 14 321 1.93e-13 70.5
MS.gene54933.t1 MTR_7g021850 25.688 327 168 14 11 288 13 313 7.13e-13 68.6
MS.gene54933.t1 MTR_7g021680 24.923 325 170 13 11 287 13 311 4.63e-11 63.2
MS.gene54933.t1 MTR_7g021820 24.923 325 170 13 11 287 13 311 4.63e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54933.t1 AT5G62420 67.405 316 102 1 1 315 1 316 2.67e-163 457
MS.gene54933.t1 AT2G37770 44.373 311 167 3 7 315 9 315 2.79e-91 275
MS.gene54933.t1 AT2G37790 43.087 311 168 5 8 315 10 314 6.34e-85 258
MS.gene54933.t1 AT1G59960 41.748 309 172 7 13 315 20 326 1.18e-84 258
MS.gene54933.t1 AT2G37760 41.290 310 172 3 8 315 10 311 8.39e-81 248
MS.gene54933.t1 AT2G37760 41.290 310 172 3 8 315 10 311 8.39e-81 248
MS.gene54933.t1 AT3G53880 41.158 311 177 3 7 315 9 315 5.42e-79 243
MS.gene54933.t1 AT1G59950 41.234 308 173 7 14 315 15 320 6.43e-75 233
MS.gene54933.t1 AT2G37760 40.210 286 161 3 8 291 10 287 2.19e-73 228
MS.gene54933.t1 AT2G37760 40.210 286 161 3 8 291 10 287 2.19e-73 228
MS.gene54933.t1 AT2G37760 42.545 275 147 4 8 280 10 275 3.51e-73 228
MS.gene54933.t1 AT5G01670 39.103 312 179 7 7 314 17 321 1.51e-71 224
MS.gene54933.t1 AT2G21250 38.487 304 169 6 6 299 3 298 2.71e-69 218
MS.gene54933.t1 AT2G21260 38.033 305 171 6 6 300 3 299 1.31e-68 216
MS.gene54933.t1 AT5G01670 36.283 339 178 8 7 314 17 348 3.14e-67 214
MS.gene54933.t1 AT3G53880 39.789 284 165 3 7 288 9 288 1.74e-66 211
MS.gene54933.t1 AT2G37760 41.538 260 148 2 58 315 8 265 5.28e-65 206
MS.gene54933.t1 AT2G37760 41.538 260 148 2 58 315 8 265 5.28e-65 206
MS.gene54933.t1 AT2G21250 38.627 233 131 5 6 228 3 233 2.39e-52 172
MS.gene54933.t1 AT2G37770 43.000 200 108 3 7 204 9 204 8.54e-51 170
MS.gene54933.t1 AT2G21260 37.768 233 129 5 78 300 5 231 1.16e-46 157
MS.gene54933.t1 AT1G06690 26.471 306 165 11 29 293 85 371 3.94e-19 87.4
MS.gene54933.t1 AT1G60710 25.705 319 170 11 22 298 33 326 7.71e-14 71.6
MS.gene54933.t1 AT5G53580 24.038 312 178 8 17 283 50 347 2.84e-13 70.1
MS.gene54933.t1 AT1G60690 24.566 346 185 14 2 297 6 325 2.72e-12 67.0
MS.gene54933.t1 AT1G60750 24.601 313 184 12 22 308 34 320 4.55e-12 66.2
MS.gene54933.t1 AT1G60730 24.611 321 179 11 22 302 33 330 7.34e-12 65.9
MS.gene54933.t1 AT1G60730 24.620 329 189 12 22 302 33 350 9.46e-12 65.5

Find 78 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATAGATTGCCTTATTTA+TGG 0.094666 4.3:-63203099 None:intergenic
TGAAGATGACTTTGAAAATT+TGG 0.094815 4.3:+63203474 MS.gene54933:CDS
AAGGCAATTGATTGTATAAT+TGG 0.200651 4.3:-63204143 None:intergenic
GAATCTGGACATGGAATATT+TGG 0.203266 4.3:+63203390 MS.gene54933:CDS
TCTGTTGTCTTCTTATCATT+TGG 0.211759 4.3:-63202497 None:intergenic
CATAAATAAGGCAATCTATA+TGG 0.224825 4.3:+63203100 MS.gene54933:CDS
TGAAAGAATGGAGGAAAATA+TGG 0.239865 4.3:+63204569 MS.gene54933:CDS
CTGTTGTCTTCTTATCATTT+GGG 0.296041 4.3:-63202496 None:intergenic
ACAATGCCTCTCATTGGATT+TGG 0.330693 4.3:+63202461 MS.gene54933:CDS
TGATATTTAATTACCTGTGC+TGG 0.338494 4.3:-63204182 None:intergenic
ACAAAACAATTGAAGAACTT+TGG 0.349387 4.3:+63204700 MS.gene54933:CDS
ATTAGGCAATGCCATAAATA+AGG 0.350442 4.3:+63203088 MS.gene54933:CDS
CAAAACAATTGAAGAACTTT+GGG 0.354545 4.3:+63204701 MS.gene54933:CDS
ATTTGAAGGTTGCATTGAAA+TGG 0.361915 4.3:+63204502 MS.gene54933:intron
TGGGGTTGCTTTGTGTTTGA+AGG 0.388559 4.3:-63204162 None:intergenic
GGCATTACAATGCCTCTCAT+TGG 0.395160 4.3:+63202455 MS.gene54933:CDS
TACACCTCCAGTTGTTAATC+AGG 0.396062 4.3:+63203612 MS.gene54933:CDS
GTTTGTTTCAATGCAGCAAC+AGG 0.398249 4.3:-63203185 None:intergenic
TTTGAAGGTTGCATTGAAAT+GGG 0.414907 4.3:+63204503 MS.gene54933:intron
GATATTTAATTACCTGTGCT+GGG 0.417731 4.3:-63204181 None:intergenic
TTAATTACCTGATTAACAAC+TGG 0.420192 4.3:-63203619 None:intergenic
TACTCACCACTTGGTGGACC+AGG 0.421010 4.3:+63204086 MS.gene54933:CDS
AAGTCATCTTCATTAGGAAC+AGG 0.433658 4.3:-63203463 None:intergenic
ATAAATAAGGCAATCTATAT+GGG 0.442614 4.3:+63203101 MS.gene54933:CDS
TTTATGGCATTGCCTAATGC+TGG 0.448486 4.3:-63203083 None:intergenic
TTGGTGGACCAGGGAATGCA+TGG 0.463271 4.3:+63204096 MS.gene54933:CDS
TATGGCTCTGAGCCAGCATT+AGG 0.474356 4.3:+63203071 MS.gene54933:CDS
GTTGATCCCCATGCATTCCC+TGG 0.477901 4.3:-63204104 None:intergenic
TTTCTTGACATCCAAATTGT+GGG 0.481755 4.3:+63203145 MS.gene54933:CDS
GCCTGTAAAGTTAAAACCAT+GGG 0.488120 4.3:+63203432 MS.gene54933:CDS
TGGATATGTACCTTGTGCAT+TGG 0.492094 4.3:+63203410 MS.gene54933:CDS
TCTATATGGGAGAAGTAGAA+AGG 0.493846 4.3:+63203114 MS.gene54933:CDS
GAAAGAATGGAGGAAAATAT+GGG 0.502628 4.3:+63204570 MS.gene54933:CDS
GGGATCATTTGATCTAAACT+TGG 0.506454 4.3:+63204590 MS.gene54933:CDS
GTCTTGAAATGGGGTTATGT+AGG 0.513056 4.3:+63203530 MS.gene54933:CDS
AAGTTTCAATGATGAAAGAA+TGG 0.517652 4.3:+63204557 MS.gene54933:CDS
TGTCTTCTTATCATTTGGGT+AGG 0.528221 4.3:-63202492 None:intergenic
ATGGGGTTATGTAGGTCCAT+TGG 0.529847 4.3:+63203538 MS.gene54933:CDS
CTATATGGGAGAAGTAGAAA+GGG 0.533515 4.3:+63203115 MS.gene54933:CDS
ATTTGGATCTTGAGACAACA+TGG 0.540374 4.3:+63203491 MS.gene54933:CDS
AATCATGTGCGTTTGAATTG+TGG 0.556437 4.3:+63202434 MS.gene54933:CDS
TCAATGCAGCAACAGGATCA+TGG 0.559666 4.3:-63203178 None:intergenic
TGGTGGACCAGGGAATGCAT+GGG 0.560104 4.3:+63204097 MS.gene54933:CDS
TTGAAATGGGGATTATCAAA+GGG 0.560330 4.3:+63204516 MS.gene54933:CDS
ATTGAAATGGGGATTATCAA+AGG 0.560489 4.3:+63204515 MS.gene54933:CDS
AAGAAATGAAGATTATGAGA+GGG 0.564196 4.3:+63204646 MS.gene54933:CDS
TGAGACAACATGGGTAGGGA+TGG 0.572572 4.3:+63203501 MS.gene54933:CDS
TTCTTGACATCCAAATTGTG+GGG 0.578334 4.3:+63203146 MS.gene54933:CDS
GGAACAGGATAGTTAACCCA+TGG 0.578853 4.3:-63203448 None:intergenic
TGGTCCACCAAGTGGTGAGT+AGG 0.583500 4.3:-63204084 None:intergenic
GATCTTGAGACAACATGGGT+AGG 0.585515 4.3:+63203496 MS.gene54933:CDS
ACACAAAGCAACCCCAGCAC+AGG 0.586444 4.3:+63204169 MS.gene54933:CDS
TTGAAGGTTGCATTGAAATG+GGG 0.588735 4.3:+63204504 MS.gene54933:intron
GAAGAAATGAAGATTATGAG+AGG 0.591725 4.3:+63204645 MS.gene54933:CDS
AGAGAAATTACTAACTCCAA+TGG 0.591742 4.3:-63203554 None:intergenic
ATCTTGAGACAACATGGGTA+GGG 0.592828 4.3:+63203497 MS.gene54933:CDS
TGCAGTCCACAATGCCCTTG+AGG 0.593505 4.3:+63202523 MS.gene54933:CDS
TCTCTAGCAACAAGATTGAG+TGG 0.593774 4.3:+63203572 MS.gene54933:CDS
TTTCAATGATGAAAGAATGG+AGG 0.594754 4.3:+63204560 MS.gene54933:CDS
GGCCTGTAAAGTTAAAACCA+TGG 0.600838 4.3:+63203431 MS.gene54933:CDS
ATATTTAATTACCTGTGCTG+GGG 0.601478 4.3:-63204180 None:intergenic
AGAAATGAAGATTATGAGAG+GGG 0.602586 4.3:+63204647 MS.gene54933:CDS
ACTTTACAGGCCAATGCACA+AGG 0.616222 4.3:-63203420 None:intergenic
GTAAGTGCCTACTCACCACT+TGG 0.619442 4.3:+63204077 MS.gene54933:CDS
AGTGCCTACTCACCACTTGG+TGG 0.619529 4.3:+63204080 MS.gene54933:CDS
AGCTTTCTCTGCCTCCACAT+AGG 0.620551 4.3:-63204023 None:intergenic
TCTTGACATCCAAATTGTGG+GGG 0.621026 4.3:+63203147 MS.gene54933:CDS
AGGTGGAAATGCATCCTATG+TGG 0.626109 4.3:+63204009 MS.gene54933:intron
ATTACCTGATTAACAACTGG+AGG 0.626966 4.3:-63203616 None:intergenic
AGAAAGCTGAGAAAGACATG+TGG 0.633349 4.3:+63204038 MS.gene54933:CDS
GATTAAGGTTAGTACCTCAA+GGG 0.643115 4.3:-63202537 None:intergenic
TAAGTGCCAAATCCAATGAG+AGG 0.655261 4.3:-63202467 None:intergenic
TGGAAATGCATCCTATGTGG+AGG 0.657202 4.3:+63204012 MS.gene54933:CDS
GGTGGACCAGGGAATGCATG+GGG 0.659494 4.3:+63204098 MS.gene54933:CDS
GCATTCCCTGGTCCACCAAG+TGG 0.660549 4.3:-63204092 None:intergenic
TTTGGATCTTGAGACAACAT+GGG 0.665864 4.3:+63203492 MS.gene54933:CDS
ACTCACCACTTGGTGGACCA+GGG 0.670084 4.3:+63204087 MS.gene54933:CDS
TTAGTACCTCAAGGGCATTG+TGG 0.678400 4.3:-63202529 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAAAAAATTAAGATAT+AGG - chr4.3:63203259-63203278 None:intergenic 10.0%
!!! ATATATTTTATTTACTTGTT+TGG - chr4.3:63204255-63204274 None:intergenic 10.0%
!!! CTATATTAATTTAAAATACA+AGG - chr4.3:63202913-63202932 None:intergenic 10.0%
!!! TAATATTTTGATATATACTT+AGG + chr4.3:63203989-63204008 MS.gene54933:intron 10.0%
!! TATCAAATTTATTTGAACAT+TGG - chr4.3:63204363-63204382 None:intergenic 15.0%
!! TTATTAAATCAACCTAAAAA+TGG - chr4.3:63203701-63203720 None:intergenic 15.0%
!!! TATAAGAAAAATCCATTTTT+AGG + chr4.3:63203686-63203705 MS.gene54933:intron 15.0%
!!! TATATTTGAGATTGAAAAAT+TGG + chr4.3:63204623-63204642 MS.gene54933:CDS 15.0%
!! AAAGATATAGATCAATTTGA+TGG + chr4.3:63202823-63202842 MS.gene54933:intron 20.0%
!! ACTGAATGAAATAACTTATT+TGG + chr4.3:63203803-63203822 MS.gene54933:intron 20.0%
!! ATAAATAAGGCAATCTATAT+GGG + chr4.3:63203101-63203120 MS.gene54933:CDS 20.0%
!! CTGAATGAAATAACTTATTT+GGG + chr4.3:63203804-63203823 MS.gene54933:intron 20.0%
!! TAATCAGGTAATTAACTAAT+TGG + chr4.3:63203627-63203646 MS.gene54933:intron 20.0%
!! TATATTTGTGTTCGATAATA+AGG - chr4.3:63203001-63203020 None:intergenic 20.0%
!! TTGAAATATTAATAGAGTCA+AGG + chr4.3:63202779-63202798 MS.gene54933:intron 20.0%
!! TTTGATACAGCAAAAATTTA+TGG + chr4.3:63203053-63203072 MS.gene54933:CDS 20.0%
!!! CAAGTTTAAGACTTTTTTTT+TGG + chr4.3:63204203-63204222 MS.gene54933:intron 20.0%
!!! TATTTTGATATATACTTAGG+TGG + chr4.3:63203992-63204011 MS.gene54933:intron 20.0%
! AACAAATGTGTATGTGATTA+AGG - chr4.3:63202555-63202574 None:intergenic 25.0%
! AAGAAATGAAGATTATGAGA+GGG + chr4.3:63204646-63204665 MS.gene54933:CDS 25.0%
! AAGGCAATTGATTGTATAAT+TGG - chr4.3:63204146-63204165 None:intergenic 25.0%
! AAGTTTCAATGATGAAAGAA+TGG + chr4.3:63204557-63204576 MS.gene54933:CDS 25.0%
! AATTGAAAGAATGTAACATG+TGG + chr4.3:63203303-63203322 MS.gene54933:intron 25.0%
! ACAAAACAATTGAAGAACTT+TGG + chr4.3:63204700-63204719 MS.gene54933:CDS 25.0%
! ATATATGTATGTGTGTATAC+AGG + chr4.3:63203016-63203035 MS.gene54933:intron 25.0%
! ATGGAAAAATGTCTTGAAAT+GGG + chr4.3:63203520-63203539 MS.gene54933:CDS 25.0%
! CAAAACAATTGAAGAACTTT+GGG + chr4.3:63204701-63204720 MS.gene54933:CDS 25.0%
! CATAAATAAGGCAATCTATA+TGG + chr4.3:63203100-63203119 MS.gene54933:CDS 25.0%
! TAATTGGAATTTATCTTGAG+TGG + chr4.3:63203643-63203662 MS.gene54933:intron 25.0%
! TCAAACTAATTTACTGATGT+TGG - chr4.3:63203903-63203922 None:intergenic 25.0%
! TGTATCCTTAGTAAAATGAA+TGG + chr4.3:63204434-63204453 MS.gene54933:intron 25.0%
! TTAATTACCTGATTAACAAC+TGG - chr4.3:63203622-63203641 None:intergenic 25.0%
! TTGACAAAAACAAAAAGGTA+GGG + chr4.3:63202718-63202737 MS.gene54933:intron 25.0%
! TTGTCAAAAGTTTCTCAATT+AGG - chr4.3:63202745-63202764 None:intergenic 25.0%
! TTTGACAAAAACAAAAAGGT+AGG + chr4.3:63202717-63202736 MS.gene54933:intron 25.0%
!! CATTACCATTCATTTTACTA+AGG - chr4.3:63204442-63204461 None:intergenic 25.0%
!! TGAAGATGACTTTGAAAATT+TGG + chr4.3:63203474-63203493 MS.gene54933:CDS 25.0%
!! TTTTCAAAGTCATCTTCATT+AGG - chr4.3:63203472-63203491 None:intergenic 25.0%
!!! AAGTTCTTCAATTGTTTTGT+AGG - chr4.3:63204700-63204719 None:intergenic 25.0%
!!! AGTTCTTCAATTGTTTTGTA+GGG - chr4.3:63204699-63204718 None:intergenic 25.0%
!!! CATATAGATTGCCTTATTTA+TGG - chr4.3:63203102-63203121 None:intergenic 25.0%
!!! CTTTTTGTTTTTGTCAAAGA+CGG - chr4.3:63202715-63202734 None:intergenic 25.0%
!!! GATTTTTTGAATGCAAAAAC+AGG + chr4.3:63203368-63203387 MS.gene54933:intron 25.0%
AGAAATGAAGATTATGAGAG+GGG + chr4.3:63204647-63204666 MS.gene54933:CDS 30.0%
AGAGAAATTACTAACTCCAA+TGG - chr4.3:63203557-63203576 None:intergenic 30.0%
ATAAGCAATGCAAAAACATG+TGG + chr4.3:63203333-63203352 MS.gene54933:intron 30.0%
ATATTTAATTACCTGTGCTG+GGG - chr4.3:63204183-63204202 None:intergenic 30.0%
ATTAGGCAATGCCATAAATA+AGG + chr4.3:63203088-63203107 MS.gene54933:CDS 30.0%
ATTGAAATGGGGATTATCAA+AGG + chr4.3:63204515-63204534 MS.gene54933:CDS 30.0%
ATTTACTGATGTTGGTATCA+AGG - chr4.3:63203895-63203914 None:intergenic 30.0%
ATTTGAAGGTTGCATTGAAA+TGG + chr4.3:63204502-63204521 MS.gene54933:intron 30.0%
CAAAGACAAGACCAATTTAA+TGG - chr4.3:63202641-63202660 None:intergenic 30.0%
CGTCTTTGACAAAAACAAAA+AGG + chr4.3:63202713-63202732 MS.gene54933:intron 30.0%
CTGTTGTCTTCTTATCATTT+GGG - chr4.3:63202499-63202518 None:intergenic 30.0%
GAAAGAATGGAGGAAAATAT+GGG + chr4.3:63204570-63204589 MS.gene54933:CDS 30.0%
GAAGAAATGAAGATTATGAG+AGG + chr4.3:63204645-63204664 MS.gene54933:CDS 30.0%
GATATTTAATTACCTGTGCT+GGG - chr4.3:63204184-63204203 None:intergenic 30.0%
GATGGAAAAATGTCTTGAAA+TGG + chr4.3:63203519-63203538 MS.gene54933:CDS 30.0%
GGATACAAATGCAACTAATT+TGG - chr4.3:63204421-63204440 None:intergenic 30.0%
TAGCGAGTAAACCATTAAAT+TGG + chr4.3:63202627-63202646 MS.gene54933:intron 30.0%
TCTGTTGTCTTCTTATCATT+TGG - chr4.3:63202500-63202519 None:intergenic 30.0%
TGAAAGAATGGAGGAAAATA+TGG + chr4.3:63204569-63204588 MS.gene54933:CDS 30.0%
TGATATTTAATTACCTGTGC+TGG - chr4.3:63204185-63204204 None:intergenic 30.0%
TGGAAAAATGTCTTGAAATG+GGG + chr4.3:63203521-63203540 MS.gene54933:CDS 30.0%
TTGAAATGGGGATTATCAAA+GGG + chr4.3:63204516-63204535 MS.gene54933:CDS 30.0%
TTTCAATGATGAAAGAATGG+AGG + chr4.3:63204560-63204579 MS.gene54933:CDS 30.0%
TTTCTTGACATCCAAATTGT+GGG + chr4.3:63203145-63203164 MS.gene54933:CDS 30.0%
TTTGAAGGTTGCATTGAAAT+GGG + chr4.3:63204503-63204522 MS.gene54933:intron 30.0%
! TTTTCTTGACATCCAAATTG+TGG + chr4.3:63203144-63203163 MS.gene54933:CDS 30.0%
!! GACTATGATCTTTGATTTGA+AGG + chr4.3:63204488-63204507 MS.gene54933:intron 30.0%
AAGTCATCTTCATTAGGAAC+AGG - chr4.3:63203466-63203485 None:intergenic 35.0%
AATCATGTGCGTTTGAATTG+TGG + chr4.3:63202434-63202453 MS.gene54933:CDS 35.0%
ATGTATGTGTGTATACAGGT+GGG + chr4.3:63203020-63203039 MS.gene54933:intron 35.0%
ATTACCTGATTAACAACTGG+AGG - chr4.3:63203619-63203638 None:intergenic 35.0%
ATTTGGATCTTGAGACAACA+TGG + chr4.3:63203491-63203510 MS.gene54933:CDS 35.0%
CTATATGGGAGAAGTAGAAA+GGG + chr4.3:63203115-63203134 MS.gene54933:CDS 35.0%
GAATCTGGACATGGAATATT+TGG + chr4.3:63203390-63203409 MS.gene54933:CDS 35.0%
GATTAAGGTTAGTACCTCAA+GGG - chr4.3:63202540-63202559 None:intergenic 35.0%
GCCTGTAAAGTTAAAACCAT+GGG + chr4.3:63203432-63203451 MS.gene54933:CDS 35.0%
GGGATCATTTGATCTAAACT+TGG + chr4.3:63204590-63204609 MS.gene54933:CDS 35.0%
TATGTATGTGTGTATACAGG+TGG + chr4.3:63203019-63203038 MS.gene54933:intron 35.0%
TCTATATGGGAGAAGTAGAA+AGG + chr4.3:63203114-63203133 MS.gene54933:CDS 35.0%
TGAATGCAAAAACAGGAATC+TGG + chr4.3:63203375-63203394 MS.gene54933:intron 35.0%
TGATTAAGGTTAGTACCTCA+AGG - chr4.3:63202541-63202560 None:intergenic 35.0%
TGTCTTCTTATCATTTGGGT+AGG - chr4.3:63202495-63202514 None:intergenic 35.0%
TTCTTGACATCCAAATTGTG+GGG + chr4.3:63203146-63203165 MS.gene54933:CDS 35.0%
TTGAAGGTTGCATTGAAATG+GGG + chr4.3:63204504-63204523 MS.gene54933:intron 35.0%
TTTGGATCTTGAGACAACAT+GGG + chr4.3:63203492-63203511 MS.gene54933:CDS 35.0%
!! AATTGTTTTGTAGGGACTTG+TGG - chr4.3:63204691-63204710 None:intergenic 35.0%
!!! ACCCATGGTTTTAACTTTAC+AGG - chr4.3:63203436-63203455 None:intergenic 35.0%
ACAATGCCTCTCATTGGATT+TGG + chr4.3:63202461-63202480 MS.gene54933:CDS 40.0%
AGAAAGCTGAGAAAGACATG+TGG + chr4.3:63204038-63204057 MS.gene54933:CDS 40.0%
ATCTTGAGACAACATGGGTA+GGG + chr4.3:63203497-63203516 MS.gene54933:CDS 40.0%
CAAAAACAGGAATCTGGACA+TGG + chr4.3:63203381-63203400 MS.gene54933:intron 40.0%
GGCCTGTAAAGTTAAAACCA+TGG + chr4.3:63203431-63203450 MS.gene54933:CDS 40.0%
GTCTTGAAATGGGGTTATGT+AGG + chr4.3:63203530-63203549 MS.gene54933:CDS 40.0%
GTTTGTTTCAATGCAGCAAC+AGG - chr4.3:63203188-63203207 None:intergenic 40.0%
TAAGTGCCAAATCCAATGAG+AGG - chr4.3:63202470-63202489 None:intergenic 40.0%
TACACCTCCAGTTGTTAATC+AGG + chr4.3:63203612-63203631 MS.gene54933:CDS 40.0%
TCTCTAGCAACAAGATTGAG+TGG + chr4.3:63203572-63203591 MS.gene54933:CDS 40.0%
TCTTGACATCCAAATTGTGG+GGG + chr4.3:63203147-63203166 MS.gene54933:CDS 40.0%
TGGATATGTACCTTGTGCAT+TGG + chr4.3:63203410-63203429 MS.gene54933:CDS 40.0%
TTTATGGCATTGCCTAATGC+TGG - chr4.3:63203086-63203105 None:intergenic 40.0%
! TTTTGTAGGGACTTGTGGTT+TGG - chr4.3:63204686-63204705 None:intergenic 40.0%
ACTTTACAGGCCAATGCACA+AGG - chr4.3:63203423-63203442 None:intergenic 45.0%
AGGTGGAAATGCATCCTATG+TGG + chr4.3:63204009-63204028 MS.gene54933:intron 45.0%
ATGGGGTTATGTAGGTCCAT+TGG + chr4.3:63203538-63203557 MS.gene54933:CDS 45.0%
GATCTTGAGACAACATGGGT+AGG + chr4.3:63203496-63203515 MS.gene54933:CDS 45.0%
GGAACAGGATAGTTAACCCA+TGG - chr4.3:63203451-63203470 None:intergenic 45.0%
GGCATTACAATGCCTCTCAT+TGG + chr4.3:63202455-63202474 MS.gene54933:CDS 45.0%
TCAATGCAGCAACAGGATCA+TGG - chr4.3:63203181-63203200 None:intergenic 45.0%
TGGAAATGCATCCTATGTGG+AGG + chr4.3:63204012-63204031 MS.gene54933:CDS 45.0%
! TGGGGTTGCTTTGTGTTTGA+AGG - chr4.3:63204165-63204184 None:intergenic 45.0%
!! TTAGTACCTCAAGGGCATTG+TGG - chr4.3:63202532-63202551 None:intergenic 45.0%
!! TTAAATTAATATAGTAAAAT+AGG + chr4.3:63202919-63202938 MS.gene54933:intron 5.0%
AGCTTTCTCTGCCTCCACAT+AGG - chr4.3:63204026-63204045 None:intergenic 50.0%
GTAAGTGCCTACTCACCACT+TGG + chr4.3:63204077-63204096 MS.gene54933:CDS 50.0%
GTGATCACTCCCCCACAATT+TGG - chr4.3:63203159-63203178 None:intergenic 50.0%
TGAGACAACATGGGTAGGGA+TGG + chr4.3:63203501-63203520 MS.gene54933:CDS 50.0%
!! TATGGCTCTGAGCCAGCATT+AGG + chr4.3:63203071-63203090 MS.gene54933:CDS 50.0%
ACACAAAGCAACCCCAGCAC+AGG + chr4.3:63204169-63204188 MS.gene54933:CDS 55.0%
AGTGCCTACTCACCACTTGG+TGG + chr4.3:63204080-63204099 MS.gene54933:CDS 55.0%
GTTGATCCCCATGCATTCCC+TGG - chr4.3:63204107-63204126 None:intergenic 55.0%
TGCAGTCCACAATGCCCTTG+AGG + chr4.3:63202523-63202542 MS.gene54933:CDS 55.0%
TGGTCCACCAAGTGGTGAGT+AGG - chr4.3:63204087-63204106 None:intergenic 55.0%
! ACTCACCACTTGGTGGACCA+GGG + chr4.3:63204087-63204106 MS.gene54933:CDS 55.0%
! TACTCACCACTTGGTGGACC+AGG + chr4.3:63204086-63204105 MS.gene54933:CDS 55.0%
! TGGTGGACCAGGGAATGCAT+GGG + chr4.3:63204097-63204116 MS.gene54933:CDS 55.0%
! TTGGTGGACCAGGGAATGCA+TGG + chr4.3:63204096-63204115 MS.gene54933:CDS 55.0%
GCATTCCCTGGTCCACCAAG+TGG - chr4.3:63204095-63204114 None:intergenic 60.0%
GGTGGACCAGGGAATGCATG+GGG + chr4.3:63204098-63204117 MS.gene54933:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 63202425 63204737 63202425 ID=MS.gene54933
chr4.3 mRNA 63202425 63204737 63202425 ID=MS.gene54933.t1;Parent=MS.gene54933
chr4.3 exon 63202425 63202544 63202425 ID=MS.gene54933.t1.exon1;Parent=MS.gene54933.t1
chr4.3 CDS 63202425 63202544 63202425 ID=cds.MS.gene54933.t1;Parent=MS.gene54933.t1
chr4.3 exon 63203038 63203213 63203038 ID=MS.gene54933.t1.exon2;Parent=MS.gene54933.t1
chr4.3 CDS 63203038 63203213 63203038 ID=cds.MS.gene54933.t1;Parent=MS.gene54933.t1
chr4.3 exon 63203390 63203633 63203390 ID=MS.gene54933.t1.exon3;Parent=MS.gene54933.t1
chr4.3 CDS 63203390 63203633 63203390 ID=cds.MS.gene54933.t1;Parent=MS.gene54933.t1
chr4.3 exon 63204011 63204190 63204011 ID=MS.gene54933.t1.exon4;Parent=MS.gene54933.t1
chr4.3 CDS 63204011 63204190 63204011 ID=cds.MS.gene54933.t1;Parent=MS.gene54933.t1
chr4.3 exon 63204510 63204737 63204510 ID=MS.gene54933.t1.exon5;Parent=MS.gene54933.t1
chr4.3 CDS 63204510 63204737 63204510 ID=cds.MS.gene54933.t1;Parent=MS.gene54933.t1
Gene Sequence

>MS.gene54933

ATGAGGTGCAATCATGTGCGTTTGAATTGTGGCATTACAATGCCTCTCATTGGATTTGGCACTTATTCCTACCCAAATGATAAGAAGACAACAGAAAATGCAGTCCACAATGCCCTTGAGGTACTAACCTTAATCACATACACATTTGTTGTATGAAGATCATATTGTTGTTATAGATTGTTGTATGAACATCAAATAACGATAGCGAGTAAACCATTAAATTGGTCTTGTCTTTGTAAGTCGTTCTTAAACTAGTCTCTGATTTTATTAAAATTTTAAACTAGTCTCCGTCTTTGACAAAAACAAAAAGGTAGGGACCTAATTGAGAAACTTTTGACAAAAACAGAGATTAATTTGAAATATTAATAGAGTCAAGGACTAATTTGAGAATAACTTACAAAGATATAGATCAATTTGATGGCTTACTCATCAAATAACTCGTATATTGAAATGTATTTTAAATCTGATATGATATTAAAGTACAACCTTGTATTTTAAATTAATATAGTAAAATAGGTGCATGTGTTTAGTCTAACTATATTGAAAATTTGCTTGCTATTGTTCTTATTTTTTCCTTATTATCGAACACAAATATATGTATGTGTGTATACAGGTGGGTTATAGACATTTTGATACAGCAAAAATTTATGGCTCTGAGCCAGCATTAGGCAATGCCATAAATAAGGCAATCTATATGGGAGAAGTAGAAAGGGAAGATATTTTCTTGACATCCAAATTGTGGGGGAGTGATCACCATGATCCTGTTGCTGCATTGAAACAAACTCTAAAGTAAGCAAATAGAAAATAAACAAAATGCATATTAATTGCATGCCTATATCTTAATTTTTTTCATTTCATGTACAAAAAAAATCTAAAATAATTGAAAGAATGTAACATGTGGTGACACAATAAGCAATGCAAAAACATGTGGAAATTTAATATTGATTTTTTGAATGCAAAAACAGGAATCTGGACATGGAATATTTGGATATGTACCTTGTGCATTGGCCTGTAAAGTTAAAACCATGGGTTAACTATCCTGTTCCTAATGAAGATGACTTTGAAAATTTGGATCTTGAGACAACATGGGTAGGGATGGAAAAATGTCTTGAAATGGGGTTATGTAGGTCCATTGGAGTTAGTAATTTCTCTAGCAACAAGATTGAGTGGCTCTTAGATTTTGCTAATACACCTCCAGTTGTTAATCAGGTAATTAACTAATTGGAATTTATCTTGAGTGGCACTTGTATCACATCACTATTATAAGAAAAATCCATTTTTAGGTTGATTTAATAATTAATCTATTTGATCTATAATATAATTCACACACATCAATTATTGAATAAACTTAAAAAGTGATTTTTCGTTTATAATAATGACTGAATGAAATAACTTATTTGGGTTAATCTGATAGTGTTTGTTTACGACTTTAGAGTGTGTTCATCTCAAGATCTCAAATTCGATTATCCTTGATACCAACATCAGTAAATTAGTTTGAGTTCTTAAGAAAATGAATAAATAAAGTAGTTTTATCATTGACAATACGAATAGAATAATTCAACAATAATATTTTGATATATACTTAGGTGGAAATGCATCCTATGTGGAGGCAGAGAAAGCTGAGAAAGACATGTGGAGAGCACAAGATACATGTAAGTGCCTACTCACCACTTGGTGGACCAGGGAATGCATGGGGATCAACAGCTGTTGTTAATCATCCAATTATACAATCAATTGCCTTCAAACACAAAGCAACCCCAGCACAGGTAATTAAATATCAAGTTTAAGACTTTTTTTTTGGAAAGATTTTTGTAATCTAGAGTTCTACCAAACAAGTAAATAAAATATATTCAAAAAATGTGAAAATTGTTTTAATCAAAATTCAAACTTGTCTATTCTTGATAGTACATAACATAATAAATAAAAATAATTACTCCAATGTTCAAATAAATTTGATACATTATTTAAATGTACAATGATATAAAATTGATATCCAAATTAGTTGCATTTGTATCCTTAGTAAAATGAATGGTAATGTATAAATAATTTAATAAGCTAATTTTGACTATGATCTTTGATTTGAAGGTTGCATTGAAATGGGGATTATCAAAGGGCTCAAGTGTGATTGTGAAAAGTTTCAATGATGAAAGAATGGAGGAAAATATGGGATCATTTGATCTAAACTTGGATGATGAAGATATATTTGAGATTGAAAAATTGGAAGAAATGAAGATTATGAGAGGGGAATTTCATGTTAACCAAACCACAAGTCCCTACAAAACAATTGAAGAACTTTGGGATGATGAAATTTGA
Protein sequence

>MS.gene54933.t1

MRCNHVRLNCGITMPLIGFGTYSYPNDKKTTENAVHNALEVGYRHFDTAKIYGSEPALGNAINKAIYMGEVEREDIFLTSKLWGSDHHDPVAALKQTLKNLDMEYLDMYLVHWPVKLKPWVNYPVPNEDDFENLDLETTWVGMEKCLEMGLCRSIGVSNFSSNKIEWLLDFANTPPVVNQVEMHPMWRQRKLRKTCGEHKIHVSAYSPLGGPGNAWGSTAVVNHPIIQSIAFKHKATPAQVALKWGLSKGSSVIVKSFNDERMEENMGSFDLNLDDEDIFEIEKLEEMKIMRGEFHVNQTTSPYKTIEELWDDEI