AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003638

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003638.t1 MTR_4g072060 98.662 299 4 0 1 299 11 309 0.0 617
MS.gene003638.t1 MTR_8g070095 42.182 275 145 5 1 271 16 280 3.50e-73 228
MS.gene003638.t1 MTR_7g063580 41.971 274 144 4 1 272 56 316 1.70e-66 212
MS.gene003638.t1 MTR_8g070115 39.781 274 151 4 1 272 16 277 1.46e-65 208
MS.gene003638.t1 MTR_0374s0050 36.913 298 172 7 1 292 20 307 4.99e-63 202
MS.gene003638.t1 MTR_4g072360 37.847 288 163 6 1 282 17 294 1.15e-62 201
MS.gene003638.t1 MTR_4g036845 38.406 276 158 4 1 274 14 279 1.49e-62 200
MS.gene003638.t1 MTR_6g073110 40.283 283 153 6 1 277 17 289 2.27e-62 200
MS.gene003638.t1 MTR_4g072320 39.146 281 155 6 1 278 17 284 4.67e-62 199
MS.gene003638.t1 MTR_4g072350 37.847 288 163 6 1 282 17 294 1.08e-61 198
MS.gene003638.t1 MTR_4g021350 40.146 274 148 5 1 272 16 275 1.99e-60 194
MS.gene003638.t1 MTR_4g021350 40.146 274 148 5 1 272 16 275 7.68e-60 193
MS.gene003638.t1 MTR_4g021450 40.214 281 141 4 1 278 17 273 8.97e-60 193
MS.gene003638.t1 MTR_4g021410 39.858 281 142 4 1 278 17 273 1.41e-58 190
MS.gene003638.t1 MTR_3g083130 35.974 303 178 6 1 298 21 312 2.35e-57 187
MS.gene003638.t1 MTR_5g097900 39.073 302 169 6 1 298 20 310 5.33e-56 183
MS.gene003638.t1 MTR_5g097910 39.073 302 169 6 1 298 20 310 5.62e-56 183
MS.gene003638.t1 MTR_5g097940 39.073 302 169 6 1 298 20 310 5.62e-56 183
MS.gene003638.t1 MTR_4g021410 40.698 258 126 4 24 278 3 236 2.63e-53 175
MS.gene003638.t1 MTR_7g070500 37.979 287 161 6 1 285 23 294 1.69e-50 169
MS.gene003638.t1 MTR_7g070500 39.273 275 150 6 1 273 23 282 2.30e-50 169
MS.gene003638.t1 MTR_4g021450 38.009 221 110 4 61 278 1 197 1.14e-35 128
MS.gene003638.t1 MTR_4g021410 38.009 221 110 4 61 278 1 197 2.56e-35 127
MS.gene003638.t1 MTR_4g021450 37.821 156 88 2 124 278 7 154 2.13e-31 115
MS.gene003638.t1 MTR_6g086545 88.000 50 6 0 48 97 36 85 2.62e-24 94.7
MS.gene003638.t1 MTR_7g114990 27.365 296 148 10 19 278 51 315 2.01e-17 81.6
MS.gene003638.t1 MTR_6g072650 34.426 122 55 1 161 282 14 110 7.52e-16 73.2
MS.gene003638.t1 MTR_7g114980 26.389 288 151 8 27 278 57 319 3.17e-15 75.5
MS.gene003638.t1 MTR_6g072710 32.407 108 70 1 176 283 2 106 6.13e-14 67.4
MS.gene003638.t1 MTR_7g021680 26.712 292 148 8 19 274 42 303 1.08e-13 70.9
MS.gene003638.t1 MTR_7g021820 26.712 292 148 8 19 274 42 303 1.08e-13 70.9
MS.gene003638.t1 MTR_1g102750 24.567 289 152 8 27 278 51 310 2.90e-13 69.7
MS.gene003638.t1 MTR_7g115010 25.540 278 147 7 27 278 55 298 4.57e-13 68.9
MS.gene003638.t1 MTR_3g092140 22.807 285 166 9 23 274 79 342 8.64e-12 65.9
MS.gene003638.t1 MTR_1g102750 24.345 267 142 6 37 272 65 302 6.24e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003638.t1 AT2G21250 85.953 299 42 0 1 299 11 309 0.0 550
MS.gene003638.t1 AT2G21260 84.615 299 46 0 1 299 11 309 0.0 541
MS.gene003638.t1 AT2G21260 83.544 237 39 0 63 299 5 241 7.37e-153 427
MS.gene003638.t1 AT2G21250 87.444 223 28 0 1 223 11 233 1.20e-149 419
MS.gene003638.t1 AT2G37790 41.304 276 147 6 1 274 16 278 5.50e-72 224
MS.gene003638.t1 AT2G37770 41.328 271 145 5 1 269 16 274 1.18e-71 224
MS.gene003638.t1 AT2G37760 39.781 274 147 4 1 272 16 273 8.10e-67 211
MS.gene003638.t1 AT2G37760 39.781 274 147 4 1 272 16 273 8.10e-67 211
MS.gene003638.t1 AT2G37760 39.781 274 147 4 1 272 16 273 9.00e-67 211
MS.gene003638.t1 AT2G37760 39.781 274 147 4 1 272 16 273 3.65e-66 209
MS.gene003638.t1 AT2G37760 39.781 274 147 4 1 272 16 273 3.65e-66 209
MS.gene003638.t1 AT5G01670 38.194 288 161 6 1 286 24 296 1.89e-65 208
MS.gene003638.t1 AT3G53880 39.051 274 153 6 1 272 16 277 1.02e-64 206
MS.gene003638.t1 AT3G53880 39.051 274 153 6 1 272 16 277 1.22e-64 206
MS.gene003638.t1 AT1G59960 37.457 291 166 6 1 285 21 301 3.72e-63 202
MS.gene003638.t1 AT5G62420 35.336 283 172 4 1 281 14 287 2.30e-61 197
MS.gene003638.t1 AT5G01670 34.921 315 161 6 1 286 24 323 1.11e-60 197
MS.gene003638.t1 AT1G59950 38.028 284 160 6 1 278 15 288 7.45e-53 176
MS.gene003638.t1 AT2G37760 37.768 233 131 3 42 272 7 227 5.40e-51 169
MS.gene003638.t1 AT2G37760 37.768 233 131 3 42 272 7 227 5.40e-51 169
MS.gene003638.t1 AT2G37770 42.564 195 101 3 1 193 16 201 6.95e-51 169
MS.gene003638.t1 AT1G60750 25.899 278 153 8 19 274 46 292 6.28e-14 71.6
MS.gene003638.t1 AT5G53580 24.913 289 153 11 23 273 80 342 1.07e-12 68.2
MS.gene003638.t1 AT1G60680 25.566 309 171 11 7 282 34 316 4.17e-12 66.2

Find 65 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCTTCAGTTAGATTATC+TGG 0.178661 4.1:-31190723 MS.gene003638:CDS
CAATCATTGGACTTGGAGTT+TGG 0.270385 4.1:-31191319 MS.gene003638:CDS
CTAATCAACCAGCCAAGTTT+TGG 0.301887 4.1:-31188595 MS.gene003638:CDS
CGGCCCAGATTTCTCTTCGT+TGG 0.313912 4.1:-31188754 MS.gene003638:CDS
CCAGGTAGTTTCCAGGGATA+TGG 0.324257 4.1:+31189813 None:intergenic
GGCCCAGATTTCTCTTCGTT+GGG 0.324480 4.1:-31188753 MS.gene003638:CDS
TGGTACTACTGACAGTGCTT+TGG 0.350027 4.1:-31189866 MS.gene003638:CDS
AGGCCTTGTTTCATCGGGCT+TGG 0.352439 4.1:-31189779 MS.gene003638:CDS
AGAGACTCACGCTGGAAGTA+TGG 0.367962 4.1:+31189035 None:intergenic
CATTCCTAAAACATCAAAAC+TGG 0.371742 4.1:-31188708 MS.gene003638:CDS
ATTTACAGCAGGCTTTATCT+TGG 0.383165 4.1:+31189073 None:intergenic
GGTACTACTGACAGTGCTTT+GGG 0.383169 4.1:-31189865 MS.gene003638:CDS
ATTATCAATTCCATCAAAAT+CGG 0.396777 4.1:-31191266 MS.gene003638:CDS
TCGCATGCCAGGTAGTTTCC+AGG 0.413644 4.1:+31189806 None:intergenic
AAGATCTATGCCCCAAAACT+TGG 0.416833 4.1:+31188583 None:intergenic
CAGCTTTGGAATTCTGATCA+TGG 0.427560 4.1:-31190779 MS.gene003638:CDS
GATGGAAGGCCTTGTTTCAT+CGG 0.446325 4.1:-31189785 MS.gene003638:CDS
ATGGAAGGCCTTGTTTCATC+GGG 0.446988 4.1:-31189784 MS.gene003638:CDS
TGGTTCGCAGCATAGGAATC+AGG 0.448672 4.1:-31189759 MS.gene003638:intron
GTTTGGTACAGAGTCATGTT+TGG 0.456307 4.1:-31188934 MS.gene003638:CDS
CAGCACCTCCAAGTGGAGTG+TGG 0.464776 4.1:+31188973 None:intergenic
GAGATTGAAAGAGAACTTCC+AGG 0.476154 4.1:-31188684 MS.gene003638:CDS
TACAAGAATGAAGCAGAAGT+TGG 0.480314 4.1:-31191127 MS.gene003638:CDS
AAGATGCCAATCATTGGACT+TGG 0.484856 4.1:-31191326 MS.gene003638:CDS
AAGGCCTTCCATCGCATGCC+AGG 0.485617 4.1:+31189795 None:intergenic
GGATTCAAGATGCCAATCAT+TGG 0.488816 4.1:-31191332 None:intergenic
GACAGTGCTTTGGGTGAAGA+TGG 0.490541 4.1:-31189856 MS.gene003638:CDS
CAAACTCCAAGTCCAATGAT+TGG 0.497148 4.1:+31191320 None:intergenic
TCGGGCTTGGTTCGCAGCAT+AGG 0.504308 4.1:-31189766 MS.gene003638:CDS
AGCACCTCCAAGTGGAGTGT+GGG 0.505998 4.1:+31188974 None:intergenic
GCAGCAAACAAAGAATGGTT+TGG 0.506549 4.1:-31188951 MS.gene003638:CDS
CCAGTGTGCCTAGTGGCTAC+AGG 0.509750 4.1:+31190680 None:intergenic
TTTGGGTGAAGATGGGGTGC+TGG 0.520967 4.1:-31189848 MS.gene003638:CDS
CTAATCAAGTTGTACCTTGG+TGG 0.525690 4.1:+31191045 None:intergenic
CTTGGAGTTTGGCGCATGGA+AGG 0.527245 4.1:-31191308 MS.gene003638:CDS
GCCCAGATTTCTCTTCGTTG+GGG 0.533029 4.1:-31188752 MS.gene003638:CDS
GTTTCAATCTGATTTACAGC+AGG 0.535497 4.1:+31189062 None:intergenic
CCATATCCCTGGAAACTACC+TGG 0.536079 4.1:-31189813 MS.gene003638:CDS
ACAGTGCTTTGGGTGAAGAT+GGG 0.536968 4.1:-31189855 MS.gene003638:CDS
AGCTCATCAACGGTATGGAC+AGG 0.538810 4.1:-31188628 MS.gene003638:CDS
TTCACTTTCCTGTAGCCACT+AGG 0.540134 4.1:-31190688 MS.gene003638:CDS
GTGCTGCAGCAAACAAAGAA+TGG 0.545070 4.1:-31188956 MS.gene003638:CDS
TGACACTGGACTTGTGAAGA+GGG 0.546697 4.1:-31191083 MS.gene003638:CDS
CCCAGATTTCTCTTCGTTGG+GGG 0.546879 4.1:-31188751 MS.gene003638:CDS
TGAGTTGTCTAAAGAGGACA+TGG 0.548008 4.1:-31188651 MS.gene003638:CDS
TCTATGCCCCAAAACTTGGC+TGG 0.554874 4.1:+31188587 None:intergenic
GTAACAGCCCACACTCCACT+TGG 0.555247 4.1:-31188981 MS.gene003638:CDS
CATAGATACAACCATATCCC+TGG 0.561415 4.1:-31189824 MS.gene003638:CDS
CATGGAGCTCATCAACGGTA+TGG 0.562792 4.1:-31188633 MS.gene003638:CDS
ACTACCTGGCATGCGATGGA+AGG 0.563810 4.1:-31189799 MS.gene003638:CDS
CGCATGCCAGGTAGTTTCCA+GGG 0.567596 4.1:+31189807 None:intergenic
TTGACACTGGACTTGTGAAG+AGG 0.573004 4.1:-31191084 MS.gene003638:CDS
GGAAACTACCTGGCATGCGA+TGG 0.573428 4.1:-31189803 MS.gene003638:CDS
ACAGCCCACACTCCACTTGG+AGG 0.575322 4.1:-31188978 MS.gene003638:CDS
CAGTGCTTTGGGTGAAGATG+GGG 0.587775 4.1:-31189854 MS.gene003638:CDS
TTTGCTGCAGCACCTCCAAG+TGG 0.593943 4.1:+31188966 None:intergenic
CCTGTAGCCACTAGGCACAC+TGG 0.596234 4.1:-31190680 MS.gene003638:intron
ACAACTCAAAATCAAATACC+TGG 0.601173 4.1:+31188666 None:intergenic
GAACCAAGCCCGATGAAACA+AGG 0.610945 4.1:+31189776 None:intergenic
TGGACTTGGAGTTTGGCGCA+TGG 0.614053 4.1:-31191312 MS.gene003638:CDS
TGATCATGGACACGTTGTCG+AGG 0.621022 4.1:-31190765 MS.gene003638:CDS
GAGGACATGGAGCTCATCAA+CGG 0.648344 4.1:-31188638 MS.gene003638:CDS
CACTGGACTTGTGAAGAGGG+AGG 0.668718 4.1:-31191080 MS.gene003638:CDS
ATTTGACTAGAGACTCACGC+TGG 0.717354 4.1:+31189027 None:intergenic
AAACATACCAGTGTGCCTAG+TGG 0.724092 4.1:+31190673 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAAATTATACTTAAGAT+GGG + chr4.1:31190683-31190702 None:intergenic 15.0%
!! ATATATAAATGCTAAAACAT+TGG - chr4.1:31189583-31189602 MS.gene003638:intron 15.0%
!! ATTGATATCTGATAATAATA+TGG - chr4.1:31189630-31189649 MS.gene003638:intron 15.0%
!! CTATTTAATGAAATTACAAT+CGG - chr4.1:31190743-31190762 MS.gene003638:CDS 15.0%
!!! ATGAATTTTACCTAATAATA+AGG - chr4.1:31190243-31190262 MS.gene003638:intron 15.0%
!!! ATTTAGGATAAAATCAATTA+TGG + chr4.1:31189037-31189056 None:intergenic 15.0%
!!! TTTTTTTACTCCTTTAAATT+TGG - chr4.1:31188691-31188710 MS.gene003638:CDS 15.0%
!! AAATTCATAGTTATCTATAG+AGG + chr4.1:31189938-31189957 None:intergenic 20.0%
!! ACTTTGATATTATGAGTTAA+TGG - chr4.1:31189072-31189091 MS.gene003638:CDS 20.0%
!! AGATTGAAATCCAAATTTAA+AGG + chr4.1:31188704-31188723 None:intergenic 20.0%
!! AGCTCAAAAAAATGTTATAA+TGG - chr4.1:31189505-31189524 MS.gene003638:intron 20.0%
!! ATTAGATACATGTGTTAAAT+AGG - chr4.1:31188870-31188889 MS.gene003638:intron 20.0%
!! ATTATCAATTCCATCAAAAT+CGG - chr4.1:31188631-31188650 MS.gene003638:CDS 20.0%
!! CATACAAATTATACTTAAGA+TGG + chr4.1:31190684-31190703 None:intergenic 20.0%
!! CTCAAAAAAATGTTATAATG+GGG - chr4.1:31189507-31189526 MS.gene003638:intron 20.0%
!! GCTCAAAAAAATGTTATAAT+GGG - chr4.1:31189506-31189525 MS.gene003638:intron 20.0%
!! TAAGCATTATTATTCCATTA+GGG + chr4.1:31190604-31190623 None:intergenic 20.0%
!! TAGAAATTGCTAATCTATTT+AGG + chr4.1:31189053-31189072 None:intergenic 20.0%
!! TATTATGAGTTAATGGTATT+TGG - chr4.1:31189079-31189098 MS.gene003638:CDS 20.0%
!! TGGTTATAAGATAAGATAAT+AGG + chr4.1:31191044-31191063 None:intergenic 20.0%
!! TTCTATACATTCTATTTACT+TGG - chr4.1:31189406-31189425 MS.gene003638:intron 20.0%
!! TTGACTATTTCCTTATTATT+AGG + chr4.1:31190256-31190275 None:intergenic 20.0%
!! TTTGTCTAATCTATTGAAAA+GGG - chr4.1:31190642-31190661 MS.gene003638:intron 20.0%
!!! ATATAACAGTTGATGATTTA+AGG + chr4.1:31189821-31189840 None:intergenic 20.0%
!!! ATTTAATTTGTATACATCGA+CGG + chr4.1:31188908-31188927 None:intergenic 20.0%
!!! CAAAGTATTGATATACTAAT+TGG - chr4.1:31189314-31189333 MS.gene003638:intron 20.0%
!!! CATCTTAAGTATAATTTGTA+TGG - chr4.1:31190682-31190701 MS.gene003638:CDS 20.0%
!!! TTTTAACTATAGCTATCAAT+AGG - chr4.1:31189471-31189490 MS.gene003638:intron 20.0%
!!! TTTTGATGGAATTGATAATT+AGG + chr4.1:31188630-31188649 None:intergenic 20.0%
! AGAAAAAGAAATGTTCAGAT+GGG + chr4.1:31189881-31189900 None:intergenic 25.0%
! ATTCATCATGATATTACGAA+CGG - chr4.1:31190460-31190479 MS.gene003638:intron 25.0%
! CATAAACTATAGTCGTAAAA+AGG + chr4.1:31190221-31190240 None:intergenic 25.0%
! CATCAAAACTAAAAAGTCAA+GGG + chr4.1:31189264-31189283 None:intergenic 25.0%
! CCTAATTCTGATTATAAAGA+AGG - chr4.1:31188928-31188947 MS.gene003638:CDS 25.0%
! CCTTCTTTATAATCAGAATT+AGG + chr4.1:31188931-31188950 None:intergenic 25.0%
! CTAAGCATTATTATTCCATT+AGG + chr4.1:31190605-31190624 None:intergenic 25.0%
! GATGAATATTGCAAAATACT+TGG + chr4.1:31190447-31190466 None:intergenic 25.0%
! GTTTGTCTAATCTATTGAAA+AGG - chr4.1:31190641-31190660 MS.gene003638:intron 25.0%
! TAGAAAAAGAAATGTTCAGA+TGG + chr4.1:31189882-31189901 None:intergenic 25.0%
! TATCTGATAATAATATGGCA+TGG - chr4.1:31189635-31189654 MS.gene003638:intron 25.0%
! TCATCAAAACTAAAAAGTCA+AGG + chr4.1:31189265-31189284 None:intergenic 25.0%
! TGAAAAATACAAAAAGACTG+CGG - chr4.1:31191123-31191142 MS.gene003638:CDS 25.0%
! TTGTCTAATCTATTGAAAAG+GGG - chr4.1:31190643-31190662 MS.gene003638:intron 25.0%
!! AATATGGATGATAGCTATTT+TGG - chr4.1:31188971-31188990 MS.gene003638:CDS 25.0%
!!! ATATGGATGATAGCTATTTT+GGG - chr4.1:31188972-31188991 MS.gene003638:CDS 25.0%
!!! ATGCACGTTTATGTTATTTT+GGG - chr4.1:31190388-31190407 MS.gene003638:intron 25.0%
!!! TACTGATTTTGGATAGATAA+TGG + chr4.1:31191064-31191083 None:intergenic 25.0%
AAAAAGAAATGTTCAGATGG+GGG + chr4.1:31189879-31189898 None:intergenic 30.0%
AACTAATGACCAAACTTGAT+AGG - chr4.1:31190294-31190313 MS.gene003638:intron 30.0%
AATAGGCTACTAATATGTGT+TGG + chr4.1:31191027-31191046 None:intergenic 30.0%
ACAACTCAAAATCAAATACC+TGG + chr4.1:31191234-31191253 None:intergenic 30.0%
ATCAACAAGGCAAACAAATA+TGG - chr4.1:31188955-31188974 MS.gene003638:CDS 30.0%
CATTATTATTCCATTAGGGT+AGG + chr4.1:31190600-31190619 None:intergenic 30.0%
CATTCCTAAAACATCAAAAC+TGG - chr4.1:31191189-31191208 MS.gene003638:intron 30.0%
GAAAAAGAAATGTTCAGATG+GGG + chr4.1:31189880-31189899 None:intergenic 30.0%
GTAATATGAAACATGTGACA+AGG + chr4.1:31190554-31190573 None:intergenic 30.0%
GTAGTTAACAACAAATGTCA+AGG - chr4.1:31189678-31189697 MS.gene003638:intron 30.0%
TAAAGAAGGAAACATCAACA+AGG - chr4.1:31188942-31188961 MS.gene003638:CDS 30.0%
TCATCAACTGTTATATGTGT+TGG - chr4.1:31189825-31189844 MS.gene003638:CDS 30.0%
TGAAAAGAGATGTTTCAGAT+GGG + chr4.1:31189742-31189761 None:intergenic 30.0%
TTGAAAAGAGATGTTTCAGA+TGG + chr4.1:31189743-31189762 None:intergenic 30.0%
TTTGAATCTGATTTCAGCTT+TGG - chr4.1:31189104-31189123 MS.gene003638:CDS 30.0%
! AAAATGTTATAATGGGGTAG+TGG - chr4.1:31189513-31189532 MS.gene003638:intron 30.0%
! AACTACGCCTATCAAAATTT+TGG - chr4.1:31189432-31189451 MS.gene003638:intron 30.0%
! AATGCTTAGTTGCTATTGAT+GGG - chr4.1:31190616-31190635 MS.gene003638:intron 30.0%
! TAATGCTTAGTTGCTATTGA+TGG - chr4.1:31190615-31190634 MS.gene003638:intron 30.0%
! TATCTAATCAAGTTGTACCT+TGG + chr4.1:31188858-31188877 None:intergenic 30.0%
! TATGTAGAAACTCTTGTTGT+TGG - chr4.1:31189285-31189304 MS.gene003638:intron 30.0%
!! ATCTGAAACATCTCTTTTCA+AGG - chr4.1:31189742-31189761 MS.gene003638:intron 30.0%
!! CATGCACGTTTATGTTATTT+TGG - chr4.1:31190387-31190406 MS.gene003638:intron 30.0%
!! TCCCAAACCAAAATTTTGAT+AGG + chr4.1:31189442-31189461 None:intergenic 30.0%
!! TCTGAAACATCTCTTTTCAA+GGG - chr4.1:31189743-31189762 MS.gene003638:intron 30.0%
!!! CTAGTCACATTTACTGATTT+TGG + chr4.1:31191075-31191094 None:intergenic 30.0%
!!! GCCTATCAAAATTTTGGTTT+GGG - chr4.1:31189438-31189457 MS.gene003638:intron 30.0%
!!! TCTTTTCAAGGGTGTTTTTA+CGG - chr4.1:31189754-31189773 MS.gene003638:intron 30.0%
!!! TGATTTTGAGTTGTCTAAAG+AGG - chr4.1:31191240-31191259 MS.gene003638:intron 30.0%
AAAAGAGATGTTTCAGATGG+GGG + chr4.1:31189740-31189759 None:intergenic 35.0%
AATAACATAAACGTGCATGC+AGG + chr4.1:31190386-31190405 None:intergenic 35.0%
GAAAAGAGATGTTTCAGATG+GGG + chr4.1:31189741-31189760 None:intergenic 35.0%
GAAGCTTCAGTTAGATTATC+TGG - chr4.1:31189174-31189193 MS.gene003638:intron 35.0%
GTTTCAATCTGATTTACAGC+AGG + chr4.1:31190838-31190857 None:intergenic 35.0%
TACAAGAATGAAGCAGAAGT+TGG - chr4.1:31188770-31188789 MS.gene003638:CDS 35.0%
TATTATTCCATTAGGGTAGG+CGG + chr4.1:31190597-31190616 None:intergenic 35.0%
TTATCTATAGAGGCCAATAG+CGG + chr4.1:31189928-31189947 None:intergenic 35.0%
! ATTCAAGACATTTTGTGTGC+TGG - chr4.1:31190526-31190545 MS.gene003638:intron 35.0%
! ATTTACAGCAGGCTTTATCT+TGG + chr4.1:31190827-31190846 None:intergenic 35.0%
! CTTAAAGAAGCTTTTGACAC+TGG - chr4.1:31188800-31188819 MS.gene003638:intron 35.0%
! GAATTGTAGGGCACATTTTA+AGG + chr4.1:31190364-31190383 None:intergenic 35.0%
! GTCAAATTTTGTCAGAAGCA+TGG - chr4.1:31190886-31190905 MS.gene003638:intron 35.0%
! TAATCAACCAGCCAAGTTTT+GGG - chr4.1:31191303-31191322 MS.gene003638:CDS 35.0%
!! ATGACGATAACCGATTTTGA+TGG + chr4.1:31188644-31188663 None:intergenic 35.0%
!! CTCTCCAGTTTTGATGTTTT+AGG + chr4.1:31191196-31191215 None:intergenic 35.0%
!!! CGCCTATCAAAATTTTGGTT+TGG - chr4.1:31189437-31189456 MS.gene003638:intron 35.0%
!!! TATCGTCATTTTGATTGTGC+AGG - chr4.1:31188655-31188674 MS.gene003638:CDS 35.0%
AAGATCTATGCCCCAAAACT+TGG + chr4.1:31191317-31191336 None:intergenic 40.0%
CAAACTCCAAGTCCAATGAT+TGG + chr4.1:31188580-31188599 None:intergenic 40.0%
CAATCATTGGACTTGGAGTT+TGG - chr4.1:31188578-31188597 MS.gene003638:CDS 40.0%
CATAGATACAACCATATCCC+TGG - chr4.1:31190073-31190092 MS.gene003638:intron 40.0%
CCAAACTTGATAGGTTCATG+AGG - chr4.1:31190303-31190322 MS.gene003638:intron 40.0%
CCTCATGAACCTATCAAGTT+TGG + chr4.1:31190306-31190325 None:intergenic 40.0%
GAGATTGAAAGAGAACTTCC+AGG - chr4.1:31191213-31191232 MS.gene003638:intron 40.0%
GCAAATAATAGCAGCGCTAT+CGG + chr4.1:31189797-31189816 None:intergenic 40.0%
GCAGCAAACAAAGAATGGTT+TGG - chr4.1:31190946-31190965 MS.gene003638:intron 40.0%
GTTTGGTACAGAGTCATGTT+TGG - chr4.1:31190963-31190982 MS.gene003638:intron 40.0%
TATTGAAAAGGGGAATGTGC+TGG - chr4.1:31190653-31190672 MS.gene003638:intron 40.0%
TGAGTTGTCTAAAGAGGACA+TGG - chr4.1:31191246-31191265 MS.gene003638:CDS 40.0%
! AATCAACCAGCCAAGTTTTG+GGG - chr4.1:31191304-31191323 MS.gene003638:CDS 40.0%
! AGTGTTGCGACTTTGATTTC+AGG - chr4.1:31191093-31191112 MS.gene003638:CDS 40.0%
! ATGACTTCTAGAAGTTGCTC+TGG - chr4.1:31190191-31190210 MS.gene003638:intron 40.0%
! CTAATCAACCAGCCAAGTTT+TGG - chr4.1:31191302-31191321 MS.gene003638:CDS 40.0%
! CTAATCAAGTTGTACCTTGG+TGG + chr4.1:31188855-31188874 None:intergenic 40.0%
! GGATCTTTTCATTACCACCA+AGG - chr4.1:31188838-31188857 MS.gene003638:intron 40.0%
! GTGTTGCGACTTTGATTTCA+GGG - chr4.1:31191094-31191113 MS.gene003638:CDS 40.0%
!! CAGCTTTGGAATTCTGATCA+TGG - chr4.1:31189118-31189137 MS.gene003638:intron 40.0%
AAACATACCAGTGTGCCTAG+TGG + chr4.1:31189227-31189246 None:intergenic 45.0%
AACGTGCATGCAGGAATTGT+AGG + chr4.1:31190377-31190396 None:intergenic 45.0%
ACGTGCATGCAGGAATTGTA+GGG + chr4.1:31190376-31190395 None:intergenic 45.0%
ACTAGGCACACTGGTATGTT+TGG - chr4.1:31189226-31189245 MS.gene003638:intron 45.0%
ATAGTGACAGACACCGCTAT+TGG - chr4.1:31189912-31189931 MS.gene003638:intron 45.0%
ATTTGACTAGAGACTCACGC+TGG + chr4.1:31190873-31190892 None:intergenic 45.0%
CATATTCCTCACACATGCAG+GGG - chr4.1:31190007-31190026 MS.gene003638:intron 45.0%
CCATATTCCTCACACATGCA+GGG - chr4.1:31190006-31190025 MS.gene003638:intron 45.0%
GGTACTACTGACAGTGCTTT+GGG - chr4.1:31190032-31190051 MS.gene003638:intron 45.0%
GTGCTGCAGCAAACAAAGAA+TGG - chr4.1:31190941-31190960 MS.gene003638:intron 45.0%
TGACACTGGACTTGTGAAGA+GGG - chr4.1:31188814-31188833 MS.gene003638:intron 45.0%
TGGTACTACTGACAGTGCTT+TGG - chr4.1:31190031-31190050 MS.gene003638:intron 45.0%
TTCACTTTCCTGTAGCCACT+AGG - chr4.1:31189209-31189228 MS.gene003638:intron 45.0%
TTGACACTGGACTTGTGAAG+AGG - chr4.1:31188813-31188832 MS.gene003638:intron 45.0%
! ACAGTGCTTTGGGTGAAGAT+GGG - chr4.1:31190042-31190061 MS.gene003638:intron 45.0%
!! ATAATGGGGTAGTGGAGAGT+GGG - chr4.1:31189521-31189540 MS.gene003638:intron 45.0%
!! ATGGAAGGCCTTGTTTCATC+GGG - chr4.1:31190113-31190132 MS.gene003638:intron 45.0%
!! GATGGAAGGCCTTGTTTCAT+CGG - chr4.1:31190112-31190131 MS.gene003638:intron 45.0%
!! TATAATGGGGTAGTGGAGAG+TGG - chr4.1:31189520-31189539 MS.gene003638:intron 45.0%
!! TGTTTCAGATGGGGGTACTT+CGG + chr4.1:31189732-31189751 None:intergenic 45.0%
AGAGACTCACGCTGGAAGTA+TGG + chr4.1:31190865-31190884 None:intergenic 50.0%
AGCTCATCAACGGTATGGAC+AGG - chr4.1:31191269-31191288 MS.gene003638:CDS 50.0%
CAGTTTACCGCCTACCCTAA+TGG - chr4.1:31190587-31190606 MS.gene003638:intron 50.0%
CATGGAGCTCATCAACGGTA+TGG - chr4.1:31191264-31191283 MS.gene003638:CDS 50.0%
CATTAGGGTAGGCGGTAAAC+TGG + chr4.1:31190589-31190608 None:intergenic 50.0%
CCAGGTAGTTTCCAGGGATA+TGG + chr4.1:31190087-31190106 None:intergenic 50.0%
CCATATCCCTGGAAACTACC+TGG - chr4.1:31190084-31190103 MS.gene003638:intron 50.0%
CCCAGATTTCTCTTCGTTGG+GGG - chr4.1:31191146-31191165 MS.gene003638:intron 50.0%
CCCCCAACGAAGAGAAATCT+GGG + chr4.1:31191149-31191168 None:intergenic 50.0%
CCCTGCATGTGTGAGGAATA+TGG + chr4.1:31190009-31190028 None:intergenic 50.0%
CTCTTCGTTGGGGGATTCAA+AGG - chr4.1:31191155-31191174 MS.gene003638:intron 50.0%
GAAATGTTCAGATGGGGGAC+AGG + chr4.1:31189874-31189893 None:intergenic 50.0%
GAACCAAGCCCGATGAAACA+AGG + chr4.1:31190124-31190143 None:intergenic 50.0%
GAGGACATGGAGCTCATCAA+CGG - chr4.1:31191259-31191278 MS.gene003638:CDS 50.0%
GCCATATTCCTCACACATGC+AGG - chr4.1:31190005-31190024 MS.gene003638:intron 50.0%
GCCCAGATTTCTCTTCGTTG+GGG - chr4.1:31191145-31191164 MS.gene003638:intron 50.0%
GGCCCAGATTTCTCTTCGTT+GGG - chr4.1:31191144-31191163 MS.gene003638:intron 50.0%
TCCCCCAACGAAGAGAAATC+TGG + chr4.1:31191150-31191169 None:intergenic 50.0%
TCTATGCCCCAAAACTTGGC+TGG + chr4.1:31191313-31191332 None:intergenic 50.0%
TGATCATGGACACGTTGTCG+AGG - chr4.1:31189132-31189151 MS.gene003638:intron 50.0%
TGGTTCGCAGCATAGGAATC+AGG - chr4.1:31190138-31190157 MS.gene003638:intron 50.0%
! CAGTGCTTTGGGTGAAGATG+GGG - chr4.1:31190043-31190062 MS.gene003638:intron 50.0%
! GACAGTGCTTTGGGTGAAGA+TGG - chr4.1:31190041-31190060 MS.gene003638:intron 50.0%
!! GTTTCAGATGGGGGTACTTC+GGG + chr4.1:31189731-31189750 None:intergenic 50.0%
!! TTTCAGATGGGGGTACTTCG+GGG + chr4.1:31189730-31189749 None:intergenic 50.0%
ACTACCTGGCATGCGATGGA+AGG - chr4.1:31190098-31190117 MS.gene003638:intron 55.0%
AGCACCTCCAAGTGGAGTGT+GGG + chr4.1:31190926-31190945 None:intergenic 55.0%
ATCATCAAGTCCCCCAGCGA+TGG - chr4.1:31189848-31189867 MS.gene003638:CDS 55.0%
CACTGGACTTGTGAAGAGGG+AGG - chr4.1:31188817-31188836 MS.gene003638:intron 55.0%
CGCATGCCAGGTAGTTTCCA+GGG + chr4.1:31190093-31190112 None:intergenic 55.0%
CGGCCCAGATTTCTCTTCGT+TGG - chr4.1:31191143-31191162 MS.gene003638:intron 55.0%
CTTGGAGTTTGGCGCATGGA+AGG - chr4.1:31188589-31188608 MS.gene003638:CDS 55.0%
GGAAACTACCTGGCATGCGA+TGG - chr4.1:31190094-31190113 MS.gene003638:intron 55.0%
GTAACAGCCCACACTCCACT+TGG - chr4.1:31190916-31190935 MS.gene003638:intron 55.0%
TACCAACCCCTGCATGTGTG+AGG + chr4.1:31190016-31190035 None:intergenic 55.0%
TCGCATGCCAGGTAGTTTCC+AGG + chr4.1:31190094-31190113 None:intergenic 55.0%
TGGACTTGGAGTTTGGCGCA+TGG - chr4.1:31188585-31188604 MS.gene003638:CDS 55.0%
TTCCTCACACATGCAGGGGT+TGG - chr4.1:31190011-31190030 MS.gene003638:intron 55.0%
TTTGCTGCAGCACCTCCAAG+TGG + chr4.1:31190934-31190953 None:intergenic 55.0%
TTTGGGTGAAGATGGGGTGC+TGG - chr4.1:31190049-31190068 MS.gene003638:intron 55.0%
!! AGGCCTTGTTTCATCGGGCT+TGG - chr4.1:31190118-31190137 MS.gene003638:intron 55.0%
!! TGTTTTTACGGTCCCTCCCC+CGG - chr4.1:31189766-31189785 MS.gene003638:CDS 55.0%
AAGGCCTTCCATCGCATGCC+AGG + chr4.1:31190105-31190124 None:intergenic 60.0%
ACAGCCCACACTCCACTTGG+AGG - chr4.1:31190919-31190938 MS.gene003638:intron 60.0%
CAGCACCTCCAAGTGGAGTG+TGG + chr4.1:31190927-31190946 None:intergenic 60.0%
CCAGTGTGCCTAGTGGCTAC+AGG + chr4.1:31189220-31189239 None:intergenic 60.0%
CCTGTAGCCACTAGGCACAC+TGG - chr4.1:31189217-31189236 MS.gene003638:intron 60.0%
! TCGGGCTTGGTTCGCAGCAT+AGG - chr4.1:31190131-31190150 MS.gene003638:intron 60.0%
AGCAGCGCTATCGGCCACAC+CGG + chr4.1:31189788-31189807 None:intergenic 65.0%
ATGGGGGACAGGCCATCGCT+GGG + chr4.1:31189863-31189882 None:intergenic 65.0%
AGCGCTATCGGCCACACCGG+GGG + chr4.1:31189785-31189804 None:intergenic 70.0%
CAGCGCTATCGGCCACACCG+GGG + chr4.1:31189786-31189805 None:intergenic 70.0%
CTATCGGCCACACCGGGGGA+GGG + chr4.1:31189781-31189800 None:intergenic 70.0%
GATGGGGGACAGGCCATCGC+TGG + chr4.1:31189864-31189883 None:intergenic 70.0%
GCAGCGCTATCGGCCACACC+GGG + chr4.1:31189787-31189806 None:intergenic 70.0%
TGGGGGACAGGCCATCGCTG+GGG + chr4.1:31189862-31189881 None:intergenic 70.0%
TTACGGTCCCTCCCCCGGTG+TGG - chr4.1:31189771-31189790 MS.gene003638:CDS 70.0%
GCTATCGGCCACACCGGGGG+AGG + chr4.1:31189782-31189801 None:intergenic 75.0%
GGGGGACAGGCCATCGCTGG+GGG + chr4.1:31189861-31189880 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31188574 31191345 31188574 ID=MS.gene003638
chr4.1 mRNA 31188574 31191345 31188574 ID=MS.gene003638.t1;Parent=MS.gene003638
chr4.1 exon 31191243 31191345 31191243 ID=MS.gene003638.t1.exon1;Parent=MS.gene003638.t1
chr4.1 CDS 31191243 31191345 31191243 ID=cds.MS.gene003638.t1;Parent=MS.gene003638.t1
chr4.1 exon 31191060 31191154 31191060 ID=MS.gene003638.t1.exon2;Parent=MS.gene003638.t1
chr4.1 CDS 31191060 31191154 31191060 ID=cds.MS.gene003638.t1;Parent=MS.gene003638.t1
chr4.1 exon 31190681 31190798 31190681 ID=MS.gene003638.t1.exon3;Parent=MS.gene003638.t1
chr4.1 CDS 31190681 31190798 31190681 ID=cds.MS.gene003638.t1;Parent=MS.gene003638.t1
chr4.1 exon 31189760 31189892 31189760 ID=MS.gene003638.t1.exon4;Parent=MS.gene003638.t1
chr4.1 CDS 31189760 31189892 31189760 ID=cds.MS.gene003638.t1;Parent=MS.gene003638.t1
chr4.1 exon 31188917 31189136 31188917 ID=MS.gene003638.t1.exon5;Parent=MS.gene003638.t1
chr4.1 CDS 31188917 31189136 31188917 ID=cds.MS.gene003638.t1;Parent=MS.gene003638.t1
chr4.1 exon 31188574 31188804 31188574 ID=MS.gene003638.t1.exon6;Parent=MS.gene003638.t1
chr4.1 CDS 31188574 31188804 31188574 ID=cds.MS.gene003638.t1;Parent=MS.gene003638.t1
Gene Sequence

>MS.gene003638

ATGCCAATCATTGGACTTGGAGTTTGGCGCATGGAAGGACAAGCAATCAAAGACCTAATTATCAATTCCATCAAAATCGGTTATCGTCATTTTGATTGTGCAGGTATTTTTTTTTTCTTTTTTTACTCCTTTAAATTTGGATTTCAATCTAGTTTTTTAGCTCATTGTTTTGAATTTTGATTGTGTTGTAGCTGACTACAAGAATGAAGCAGAAGTTGGAGAAGCACTTAAAGAAGCTTTTGACACTGGACTTGTGAAGAGGGAGGATCTTTTCATTACCACCAAGGTACAACTTGATTAGATACATGTGTTAAATAGGTTTACATTTACACCGTCGATGTATACAAATTAAATCCTAATTCTGATTATAAAGAAGGAAACATCAACAAGGCAAACAAATATGGATGATAGCTATTTTGGGAAGTTGAGCTAGACAAGTGTTTCAAGCAGTCAAATTCATCCATAATTGATTTTATCCTAAATAGATTAGCAATTTCTACTTTGATATTATGAGTTAATGGTATTTGGCATTTGAATCTGATTTCAGCTTTGGAATTCTGATCATGGACACGTTGTCGAGGCTTGCAAAGATAGTCTTAAGAAGCTTCAGTTAGATTATCTGGATTTATATCTTGTTCACTTTCCTGTAGCCACTAGGCACACTGGTATGTTTGGTTTCTTATGCTTCCCTTGACTTTTTAGTTTTGATGATATGTAGAAACTCTTGTTGTTGGCTTTCTCAAAGTATTGATATACTAATTGGAGTCATATATATATAGTTAAAATATATCTACTTTTAGTCAAATCTGCTTTATGCTTTTTAATAAAATATTTCTATACATTCTATTTACTTGGTGAAACTACGCCTATCAAAATTTTGGTTTGGGATATTTTAAGTTTTAACTATAGCTATCAATAGGCATGAAGAAGCAGCTCAAAAAAATGTTATAATGGGGTAGTGGAGAGTGGGATGACGTGACATGATGTTTTTTAACATTTTTTCTTATATATATATAAATGCTAAAACATTGGAAAATTACTCATGATTCATGAACTATTGATATCTGATAATAATATGGCATGGCTCTTAAGCAAAACATAGAAGTAGTTAACAACAAATGTCAAGGAAACAAGAGAAGTTTAAGTCTAAAATCCCCGAAGTACCCCCATCTGAAACATCTCTTTTCAAGGGTGTTTTTACGGTCCCTCCCCCGGTGTGGCCGATAGCGCTGCTATTATTTGCACCTTAAATCATCAACTGTTATATGTGTTGGATCATCAAGTCCCCCAGCGATGGCCTGTCCCCCATCTGAACATTTCTTTTTCTACAGCTAGAGAATAGTGACAGACACCGCTATTGGCCTCTATAGATAACTATGAATTTAACAAGTGATCTAAAACACTCTTATATGTGTTCTATAATATAATTTTGCCATATTCCTCACACATGCAGGGGTTGGTACTACTGACAGTGCTTTGGGTGAAGATGGGGTGCTGGACATAGATACAACCATATCCCTGGAAACTACCTGGCATGCGATGGAAGGCCTTGTTTCATCGGGCTTGGTTCGCAGCATAGGAATCAGGTCTAATTTTGTGCTGCAAGCAATCTTCACTATGACTTCTAGAAGTTGCTCTGGAAATCCTTTTTACGACTATAGTTTATGTGATGAATTTTACCTAATAATAAGGAAATAGTCAAATGAACAAAGATGAAAACAACTAATGACCAAACTTGATAGGTTCATGAGGACTGAAATTATCTCTCTAGTAATTATTCAGACGCGCCTTAAAATGTGCCCTACAATTCCTGCATGCACGTTTATGTTATTTTGGGATGATTACTTGCAACATATGTTAAAAGACTTAACCAAGTATTTTGCAATATTCATCATGATATTACGAACGGTCTCAGTAATTAAGAAGTGAATTGTACTGACATTTACAGACATATTCAAGACATTTTGTGTGCTGGATCCTTGTCACATGTTTCATATTACATTTAGCTATTGCCAGTTTACCGCCTACCCTAATGGAATAATAATGCTTAGTTGCTATTGATGGGCTGTTTGTCTAATCTATTGAAAAGGGGAATGTGCTGGATAACCCATCTTAAGTATAATTTGTATGGTCTAATATTCATGTTATTGACTTCAGAAATCTTTGATTCTATTTAATGAAATTACAATCGGACAAAAACTATTTGCAGCAACTATGACATCTTTCTGACTAGAGATTGCTTAGCATATTCCAAGATAAAGCCTGCTGTAAATCAGATTGAAACTCATCCATACTTCCAGCGTGAGTCTCTAGTCAAATTTTGTCAGAAGCATGGAATTTGTGTAACAGCCCACACTCCACTTGGAGGTGCTGCAGCAAACAAAGAATGGTTTGGTACAGAGTCATGTTTGGATGAGCAAACTCTCAAAGTGAGTTGTCATTAACAAGTTCCAACACATATTAGTAGCCTATTATCTTATCTTATAACCATTATCTATCCAAAATCAGTAAATGTGACTAGTGTTGCGACTTTGATTTCAGGGTCTAGCTGAAAAATACAAAAAGACTGCGGCCCAGATTTCTCTTCGTTGGGGGATTCAAAGGAACACTGTTGTCATTCCTAAAACATCAAAACTGGAGAGATTGAAAGAGAACTTCCAGGTATTTGATTTTGAGTTGTCTAAAGAGGACATGGAGCTCATCAACGGTATGGACAGGAAATATCGAACTAATCAACCAGCCAAGTTTTGGGGCATAGATCTTTATGCATGA
Protein sequence

>MS.gene003638.t1

MPIIGLGVWRMEGQAIKDLIINSIKIGYRHFDCAADYKNEAEVGEALKEAFDTGLVKREDLFITTKLWNSDHGHVVEACKDSLKKLQLDYLDLYLVHFPVATRHTGVGTTDSALGEDGVLDIDTTISLETTWHAMEGLVSSGLVRSIGISNYDIFLTRDCLAYSKIKPAVNQIETHPYFQRESLVKFCQKHGICVTAHTPLGGAAANKEWFGTESCLDEQTLKGLAEKYKKTAAQISLRWGIQRNTVVIPKTSKLERLKENFQVFDFELSKEDMELINGMDRKYRTNQPAKFWGIDLYA