AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022560.t1 MTR_8g005295 92.816 515 34 1 1 515 1 512 0.0 984
MS.gene022560.t1 MTR_8g005285 88.155 515 44 1 1 515 1 498 0.0 929
MS.gene022560.t1 MTR_8g005325 50.398 502 179 9 19 515 38 474 3.98e-162 469
MS.gene022560.t1 MTR_8g005235 49.603 504 179 11 19 515 9 444 4.01e-159 460
MS.gene022560.t1 MTR_0209s0060 46.263 495 181 13 19 508 11 425 3.57e-134 395
MS.gene022560.t1 MTR_0209s0030 46.263 495 181 13 19 508 11 425 3.57e-134 395
MS.gene022560.t1 MTR_8g006525 46.245 506 188 13 12 512 4 430 1.14e-132 392
MS.gene022560.t1 MTR_8g005265 45.436 493 191 14 36 514 27 455 5.79e-128 381
MS.gene022560.t1 MTR_8g005225 42.748 524 223 12 1 513 1 458 6.00e-123 368
MS.gene022560.t1 MTR_3g079390 33.468 496 247 16 17 503 20 441 2.37e-64 216
MS.gene022560.t1 MTR_6g010920 31.557 488 220 17 32 508 21 405 1.13e-61 207
MS.gene022560.t1 MTR_6g009590 30.346 491 226 16 22 497 7 396 5.65e-60 203
MS.gene022560.t1 MTR_6g011050 30.297 472 218 14 36 497 24 394 5.29e-58 198
MS.gene022560.t1 MTR_4g112660 38.824 340 118 9 188 510 90 356 2.05e-57 195
MS.gene022560.t1 MTR_4g112660 46.429 84 41 3 36 118 6 86 6.06e-15 76.6
MS.gene022560.t1 MTR_6g009610 30.947 475 226 14 29 497 18 396 8.77e-57 195
MS.gene022560.t1 MTR_6g011060 29.555 494 236 15 25 503 14 410 3.41e-56 194
MS.gene022560.t1 MTR_0024s0080 30.538 465 218 14 38 497 27 391 2.43e-52 183
MS.gene022560.t1 MTR_6g010890 29.492 512 240 19 19 508 10 422 1.10e-51 182
MS.gene022560.t1 MTR_3g107590 30.526 475 225 14 34 496 24 405 1.80e-51 181
MS.gene022560.t1 MTR_6g010810 29.424 486 229 16 35 508 21 404 3.79e-49 174
MS.gene022560.t1 MTR_6g009170 29.577 497 230 17 22 496 7 405 1.53e-48 173
MS.gene022560.t1 MTR_2g036320 27.557 479 263 14 29 505 61 457 8.41e-44 162
MS.gene022560.t1 MTR_2g041180 27.331 472 261 13 36 505 50 441 2.91e-42 157
MS.gene022560.t1 MTR_2g035640 27.189 434 219 9 73 503 1 340 4.85e-38 143
MS.gene022560.t1 MTR_5g086340 25.684 475 272 10 37 508 33 429 3.62e-34 134
MS.gene022560.t1 MTR_5g016570 27.311 476 258 14 32 488 97 503 2.88e-32 130
MS.gene022560.t1 MTR_6g010850 28.889 405 181 14 109 503 33 340 7.46e-32 126
MS.gene022560.t1 MTR_7g011980 34.211 190 101 3 316 503 166 333 1.40e-25 108
MS.gene022560.t1 MTR_7g011980 31.579 171 66 4 29 199 18 137 6.37e-17 82.8
MS.gene022560.t1 MTR_7g060310 24.025 487 295 14 46 503 39 479 1.66e-23 103
MS.gene022560.t1 MTR_7g059540 22.397 509 273 15 38 503 29 458 2.28e-22 100
MS.gene022560.t1 MTR_6g009580 30.841 214 119 4 289 496 29 219 1.14e-21 94.4
MS.gene022560.t1 MTR_7g059540 22.287 516 275 16 38 503 28 467 1.87e-21 97.8
MS.gene022560.t1 MTR_7g060260 22.532 466 256 12 38 488 28 403 3.44e-19 90.5
MS.gene022560.t1 MTR_7g060115 22.822 482 269 15 53 505 44 451 3.70e-18 87.4
MS.gene022560.t1 MTR_7g060320 22.774 483 272 16 38 488 28 441 6.25e-18 86.7
MS.gene022560.t1 MTR_5g086230 30.939 181 103 4 37 213 29 191 7.65e-18 82.4
MS.gene022560.t1 MTR_7g059525 28.877 187 113 2 318 503 108 275 5.66e-17 81.6
MS.gene022560.t1 MTR_0182s0060 24.852 507 303 17 40 503 37 508 7.20e-17 84.0
MS.gene022560.t1 MTR_6g009600 32.593 135 67 2 318 451 23 134 1.26e-16 77.4
MS.gene022560.t1 MTR_7g059520 30.409 171 99 3 318 487 5 156 1.33e-16 78.2
MS.gene022560.t1 MTR_7g060080 32.432 148 95 2 363 505 42 189 1.70e-16 78.6
MS.gene022560.t1 MTR_7g446270 23.780 492 273 20 38 488 31 461 8.32e-16 80.5
MS.gene022560.t1 MTR_7g060220 23.191 470 259 14 38 488 27 413 1.14e-15 79.7
MS.gene022560.t1 MTR_5g087580 24.747 198 125 2 316 512 41 215 3.51e-15 75.1
MS.gene022560.t1 MTR_4g009340 31.176 170 104 4 36 205 42 198 5.90e-15 77.8
MS.gene022560.t1 MTR_4g009340 27.841 176 103 4 315 489 376 528 6.70e-11 65.1
MS.gene022560.t1 MTR_7g059480 32.394 142 95 1 363 503 624 765 6.22e-15 78.2
MS.gene022560.t1 MTR_7g060840 31.333 150 92 3 367 515 287 426 6.58e-15 77.4
MS.gene022560.t1 MTR_5g086260 28.108 185 107 4 318 500 136 296 7.34e-15 75.9
MS.gene022560.t1 MTR_7g060020 33.803 142 90 3 367 505 291 431 1.00e-14 76.6
MS.gene022560.t1 MTR_4g009620 31.765 170 102 4 36 205 42 197 7.11e-14 74.7
MS.gene022560.t1 MTR_4g009590 27.895 190 124 4 36 225 42 218 1.05e-13 73.9
MS.gene022560.t1 MTR_4g009590 29.104 134 94 1 373 505 411 544 8.80e-12 67.8
MS.gene022560.t1 MTR_8g042170 31.788 151 89 2 367 503 299 449 2.42e-13 72.4
MS.gene022560.t1 MTR_7g060160 33.333 147 84 5 367 505 263 403 4.93e-13 71.2
MS.gene022560.t1 MTR_2g036310 29.927 137 93 2 371 505 16 151 1.23e-12 66.2
MS.gene022560.t1 MTR_7g017620 28.788 198 126 6 4 200 9 192 2.70e-12 69.7
MS.gene022560.t1 MTR_7g017620 30.075 133 91 2 371 502 399 530 3.11e-11 66.2
MS.gene022560.t1 MTR_7g067750 27.111 225 139 9 2 218 26 233 4.78e-12 68.6
MS.gene022560.t1 MTR_7g059810 24.249 466 248 18 46 488 38 421 7.74e-12 67.8
MS.gene022560.t1 MTR_8g042830 21.687 332 200 9 184 504 124 406 9.45e-12 67.4
MS.gene022560.t1 MTR_7g059510 28.369 141 98 2 369 506 282 422 1.68e-11 66.6
MS.gene022560.t1 MTR_3g466740 27.320 194 122 6 37 230 31 205 2.30e-11 66.2
MS.gene022560.t1 MTR_7g067600 27.273 176 114 3 37 209 29 193 6.82e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022560.t1 AT2G44930 30.162 494 239 9 36 508 84 492 6.25e-56 195
MS.gene022560.t1 AT3G60470 29.870 539 269 15 38 508 34 531 4.77e-55 194
MS.gene022560.t1 AT2G28580 28.921 491 244 10 36 508 41 444 3.98e-52 184
MS.gene022560.t1 AT2G28580 28.921 491 244 10 36 508 109 512 9.95e-52 184
MS.gene022560.t1 AT2G28580 28.803 493 242 11 36 508 123 526 1.16e-51 184
MS.gene022560.t1 AT2G28580 29.167 480 240 9 36 508 109 495 3.21e-51 182
MS.gene022560.t1 AT2G28580 29.046 482 238 10 36 508 123 509 3.59e-51 183
MS.gene022560.t1 AT4G31980 27.660 470 231 12 36 496 293 662 8.85e-47 173
MS.gene022560.t1 AT5G11290 25.519 482 265 15 24 503 22 411 4.55e-40 150
MS.gene022560.t1 AT1G67150 27.178 482 246 12 35 503 5 394 3.84e-39 147
MS.gene022560.t1 AT1G67150 27.178 482 246 12 35 503 5 394 3.84e-39 147
MS.gene022560.t1 AT2G36430 25.055 455 259 11 36 488 45 419 1.16e-38 147
MS.gene022560.t1 AT3G50150 27.754 472 269 11 38 505 92 495 1.43e-38 147
MS.gene022560.t1 AT1G67150 26.955 486 246 12 35 503 5 398 1.54e-38 145
MS.gene022560.t1 AT3G50170 25.819 519 304 13 2 503 69 523 1.09e-37 145
MS.gene022560.t1 AT3G47250 26.176 489 273 13 27 503 52 464 9.33e-37 142
MS.gene022560.t1 AT3G47250 26.176 489 273 13 27 503 52 464 9.33e-37 142
MS.gene022560.t1 AT3G47250 26.176 489 273 13 27 503 52 464 9.33e-37 142
MS.gene022560.t1 AT3G47210 28.166 458 220 12 25 467 82 445 1.81e-35 138
MS.gene022560.t1 AT3G50140 26.273 491 259 14 38 505 115 525 3.08e-34 136
MS.gene022560.t1 AT3G50120 26.383 470 283 12 38 503 105 515 3.00e-33 133
MS.gene022560.t1 AT2G28580 27.488 422 197 11 36 437 123 455 6.29e-33 131
MS.gene022560.t1 AT3G50130 26.432 454 270 12 38 488 141 533 3.56e-31 127
MS.gene022560.t1 AT3G50180 25.667 487 259 16 32 503 174 572 8.86e-29 120
MS.gene022560.t1 AT3G47200 25.261 479 262 13 25 488 35 432 1.66e-27 115
MS.gene022560.t1 AT3G47200 25.261 479 262 13 25 488 35 432 1.66e-27 115
MS.gene022560.t1 AT5G22540 23.226 465 257 13 36 488 31 407 2.63e-26 111
MS.gene022560.t1 AT3G44710 24.280 486 306 14 36 503 47 488 4.11e-25 108
MS.gene022560.t1 AT3G50190 23.789 475 258 13 38 503 98 477 6.93e-23 102
MS.gene022560.t1 AT5G11290 23.326 433 239 15 73 503 1 342 1.39e-22 99.8
MS.gene022560.t1 AT1G65985 34.054 185 79 4 38 204 16 175 3.98e-20 93.6
MS.gene022560.t1 AT1G65985 37.903 124 76 1 368 490 304 427 1.54e-16 82.4
MS.gene022560.t1 AT1G65985 34.054 185 79 4 38 204 16 175 3.98e-20 93.6
MS.gene022560.t1 AT1G65985 37.903 124 76 1 368 490 304 427 1.54e-16 82.4
MS.gene022560.t1 AT3G50170 29.439 214 121 5 2 205 69 262 1.32e-19 91.7
MS.gene022560.t1 AT5G22550 31.579 209 119 8 2 206 9 197 1.73e-19 91.7
MS.gene022560.t1 AT3G50160 31.285 179 103 4 32 210 100 258 1.86e-18 88.6
MS.gene022560.t1 AT3G50160 27.679 224 133 3 286 503 285 485 8.67e-17 83.6
MS.gene022560.t1 AT1G67150 29.947 187 107 3 318 503 188 351 2.29e-18 87.4
MS.gene022560.t1 AT1G67150 31.694 183 94 5 35 207 5 166 3.20e-15 77.8
MS.gene022560.t1 AT3G50150 31.429 175 99 4 38 210 92 247 9.42e-17 83.2
MS.gene022560.t1 AT3G50140 30.952 168 96 4 38 205 115 262 6.78e-16 80.1
MS.gene022560.t1 AT5G22560 26.882 186 103 4 36 207 47 213 3.15e-15 78.6
MS.gene022560.t1 AT3G50190 27.717 184 110 5 38 221 98 258 6.27e-13 70.9
MS.gene022560.t1 AT3G50190 27.717 184 110 5 38 221 98 258 6.27e-13 70.9
MS.gene022560.t1 AT3G50190 27.717 184 110 5 38 221 98 258 7.36e-13 70.5
MS.gene022560.t1 AT3G02645 32.203 118 79 1 373 489 380 497 8.13e-13 71.2
MS.gene022560.t1 AT3G44700 26.389 144 99 4 5 143 16 157 8.52e-11 62.4

Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGCATTATCAGAATATT+TGG 0.154201 8.4:+80442856 MS.gene022560:CDS
CTTGATACTGACATTGTTCT+AGG 0.157660 8.4:-80442635 None:intergenic
ATTAACTTTGTTACTGTTCT+TGG 0.162375 8.4:-80442411 None:intergenic
ACCATTTCAAAGCGCGTCTA+AGG 0.318480 8.4:+80442556 MS.gene022560:CDS
ACGTGAAACTAAGCTTGTTT+TGG 0.326591 8.4:+80442176 MS.gene022560:CDS
GAAACATAATTGCATATATA+AGG 0.329067 8.4:-80442663 None:intergenic
GACTTTGTAGTTCTTGATCA+TGG 0.348123 8.4:-80441746 None:intergenic
TCTTTATTTCCAAGGTGAAT+TGG 0.350401 8.4:-80441646 None:intergenic
ATCAAATCAGTGAAATGTTT+AGG 0.353279 8.4:-80442324 None:intergenic
CTGCTAAACTTAACTCTTCA+AGG 0.360388 8.4:-80441543 None:intergenic
TTACCTTCCAGCTGATTTAA+CGG 0.361070 8.4:+80442356 MS.gene022560:CDS
AACATTTCACTGATTTGATA+AGG 0.390830 8.4:+80442328 MS.gene022560:CDS
ACTCCTCAATTAATCTCAAT+TGG 0.392658 8.4:+80441622 MS.gene022560:CDS
GTAACAAAGTTAATAGATTC+TGG 0.396787 8.4:+80442420 MS.gene022560:CDS
AAGAAACCGTTCATGGAAAT+CGG 0.400354 8.4:+80441891 MS.gene022560:CDS
GAAGTGGCAGTGCTTGTTAA+TGG 0.401978 8.4:+80442774 MS.gene022560:CDS
ATAGACGCTCATAGAAGAAA+TGG 0.406927 8.4:-80441705 None:intergenic
TCCTTAGACGCGCTTTGAAA+TGG 0.415205 8.4:-80442557 None:intergenic
ACAAGTTGATGTTGATTTGT+TGG 0.429548 8.4:+80442710 MS.gene022560:CDS
TCTAAATCCGTTAAATCAGC+TGG 0.442093 8.4:-80442363 None:intergenic
AGCAATAACCATAGGTTTCA+AGG 0.443493 8.4:+80442300 MS.gene022560:CDS
CAGAGAAAATAACTCAGAAA+TGG 0.447228 8.4:-80442501 None:intergenic
AATCAACATCAACTTGTGTA+TGG 0.449318 8.4:-80442704 None:intergenic
ATCTGAAACTGAATATGAAA+TGG 0.454211 8.4:+80441495 MS.gene022560:CDS
GTTTAGGACCTTGAAACCTA+TGG 0.455366 8.4:-80442308 None:intergenic
AGAGCTAGTTCTACTTTGGT+TGG 0.457619 8.4:+80442927 MS.gene022560:CDS
GGAAGTGATTGTGCATGAAA+TGG 0.461128 8.4:+80442740 MS.gene022560:CDS
GGACCAATTGAGATTAATTG+AGG 0.464532 8.4:-80441625 None:intergenic
TTGGAGAGCTAGTTCTACTT+TGG 0.466290 8.4:+80442923 MS.gene022560:CDS
AGTGGCTTAATTGGTGATGT+TGG 0.493716 8.4:+80442231 MS.gene022560:CDS
GTTGAAGTTCTTTATTTCCA+AGG 0.495312 8.4:-80441654 None:intergenic
TCTAAGGATTCCGCAGTTGA+AGG 0.499213 8.4:+80442572 MS.gene022560:CDS
AGCAGATTGTAGCATCTACA+AGG 0.504878 8.4:+80441561 MS.gene022560:CDS
ATTACATTCTAACACAAGCT+TGG 0.511928 8.4:+80441977 MS.gene022560:CDS
TTGATACTGACATTGTTCTA+GGG 0.522905 8.4:-80442634 None:intergenic
ATGTTTCAATCTAAATGACT+TGG 0.523292 8.4:-80441947 None:intergenic
GAAGTGATTGTGCATGAAAT+GGG 0.523677 8.4:+80442741 MS.gene022560:CDS
TTAACGGATTTAGAAAAGAG+TGG 0.543465 8.4:+80442372 MS.gene022560:CDS
CACCAACCACAATGCAGCCA+TGG 0.546124 8.4:-80442886 None:intergenic
TGGTTCAAACATGAAAATGA+AGG 0.548134 8.4:-80442537 None:intergenic
TCAATTGGTCCAATTCACCT+TGG 0.558480 8.4:+80441637 MS.gene022560:CDS
TTAATTGGTGATGTTGGTAG+TGG 0.564871 8.4:+80442237 MS.gene022560:CDS
ACCACAATGCAGCCATGGTA+CGG 0.571593 8.4:-80442881 None:intergenic
ATGTATCCGATTTCCATGAA+CGG 0.580945 8.4:-80441897 None:intergenic
TGGTCTTTGTAAACATGTTG+TGG 0.582048 8.4:+80442794 MS.gene022560:CDS
AATCTATGAGCAATAACCAT+AGG 0.583238 8.4:+80442292 MS.gene022560:CDS
GACATTGTTCTAGGGCAATG+AGG 0.593451 8.4:-80442626 None:intergenic
GGCTCAGCAAAAGCATCACA+AGG 0.605591 8.4:+80441998 MS.gene022560:CDS
TGTTGATTTGTTGGTCGAGA+AGG 0.609668 8.4:+80442719 MS.gene022560:CDS
TACCATGGCTGCATTGTGGT+TGG 0.611964 8.4:+80442884 MS.gene022560:CDS
AATCCGTTAAATCAGCTGGA+AGG 0.622325 8.4:-80442359 None:intergenic
AATGGGAAGTGATAAAGAAG+TGG 0.638138 8.4:+80442758 MS.gene022560:CDS
GAATATTTGGAACCGTACCA+TGG 0.643141 8.4:+80442869 MS.gene022560:CDS
GTGTGATCAACCTTCAACTG+CGG 0.710202 8.4:-80442582 None:intergenic
GCTCAGCAAAAGCATCACAA+GGG 0.717992 8.4:+80441999 MS.gene022560:CDS
ACCGTACCATGGCTGCATTG+TGG 0.722473 8.4:+80442880 MS.gene022560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGCATTATCAGAATATT+TGG + chr8.4:80442856-80442875 MS.gene022560:CDS 20.0%
!! GAAACATAATTGCATATATA+AGG - chr8.4:80442666-80442685 None:intergenic 20.0%
!!! AAATTTTTCTTTGCTTATGT+TGG - chr8.4:80441824-80441843 None:intergenic 20.0%
!!! AAGAAATATTATTTTGTGAG+TGG + chr8.4:80442213-80442232 MS.gene022560:CDS 20.0%
!!! AATTTTTCTTTGCTTATGTT+GGG - chr8.4:80441823-80441842 None:intergenic 20.0%
!!! TTTATCAGAATGTTTTTTCT+TGG - chr8.4:80442274-80442293 None:intergenic 20.0%
! ATCAAATCAGTGAAATGTTT+AGG - chr8.4:80442327-80442346 None:intergenic 25.0%
! ATGTTTCAATCTAAATGACT+TGG - chr8.4:80441950-80441969 None:intergenic 25.0%
! ATTAACTTTGTTACTGTTCT+TGG - chr8.4:80442414-80442433 None:intergenic 25.0%
! CATAAGCAAAGAAAAATTTG+TGG + chr8.4:80441825-80441844 MS.gene022560:CDS 25.0%
! GTAACAAAGTTAATAGATTC+TGG + chr8.4:80442420-80442439 MS.gene022560:CDS 25.0%
!! ATCTGAAACTGAATATGAAA+TGG + chr8.4:80441495-80441514 MS.gene022560:CDS 25.0%
!! ATTTGTGGAAACAATTTTGT+TGG + chr8.4:80441840-80441859 MS.gene022560:CDS 25.0%
!! CAAGATCAAGAAAACTTTTA+TGG - chr8.4:80442113-80442132 None:intergenic 25.0%
!!! AACATTTCACTGATTTGATA+AGG + chr8.4:80442328-80442347 MS.gene022560:CDS 25.0%
AATCAACATCAACTTGTGTA+TGG - chr8.4:80442707-80442726 None:intergenic 30.0%
AATCTATGAGCAATAACCAT+AGG + chr8.4:80442292-80442311 MS.gene022560:CDS 30.0%
ACTCCTCAATTAATCTCAAT+TGG + chr8.4:80441622-80441641 MS.gene022560:CDS 30.0%
AGATTCATCAGCACAAAAAA+AGG - chr8.4:80442051-80442070 None:intergenic 30.0%
ATTACATTCTAACACAAGCT+TGG + chr8.4:80441977-80441996 MS.gene022560:CDS 30.0%
CAATGCAACAACACAAAAAA+AGG + chr8.4:80441677-80441696 MS.gene022560:CDS 30.0%
CAGAGAAAATAACTCAGAAA+TGG - chr8.4:80442504-80442523 None:intergenic 30.0%
TGGTTCAAACATGAAAATGA+AGG - chr8.4:80442540-80442559 None:intergenic 30.0%
TTGATACTGACATTGTTCTA+GGG - chr8.4:80442637-80442656 None:intergenic 30.0%
! ACACAACAGAATGTGTTTTA+AGG + chr8.4:80442601-80442620 MS.gene022560:CDS 30.0%
! GTTGAAGTTCTTTATTTCCA+AGG - chr8.4:80441657-80441676 None:intergenic 30.0%
! TATTTTGTGAGTGGCTTAAT+TGG + chr8.4:80442222-80442241 MS.gene022560:CDS 30.0%
! TCTTTATTTCCAAGGTGAAT+TGG - chr8.4:80441649-80441668 None:intergenic 30.0%
! TTTTGTTAAGAAACCGTTCA+TGG + chr8.4:80441884-80441903 MS.gene022560:CDS 30.0%
!! AAGCTTCTTGTTTCACTTTT+CGG - chr8.4:80441600-80441619 None:intergenic 30.0%
!! ACAAGTTGATGTTGATTTGT+TGG + chr8.4:80442710-80442729 MS.gene022560:CDS 30.0%
!! TTAACGGATTTAGAAAAGAG+TGG + chr8.4:80442372-80442391 MS.gene022560:CDS 30.0%
!!! CTTTTTTTGTGTTGTTGCAT+TGG - chr8.4:80441679-80441698 None:intergenic 30.0%
AAGAAACCGTTCATGGAAAT+CGG + chr8.4:80441891-80441910 MS.gene022560:CDS 35.0%
AATGGGAAGTGATAAAGAAG+TGG + chr8.4:80442758-80442777 MS.gene022560:CDS 35.0%
AGCAATAACCATAGGTTTCA+AGG + chr8.4:80442300-80442319 MS.gene022560:CDS 35.0%
ATAGACGCTCATAGAAGAAA+TGG - chr8.4:80441708-80441727 None:intergenic 35.0%
ATGTATCCGATTTCCATGAA+CGG - chr8.4:80441900-80441919 None:intergenic 35.0%
CTGCTAAACTTAACTCTTCA+AGG - chr8.4:80441546-80441565 None:intergenic 35.0%
CTTGATACTGACATTGTTCT+AGG - chr8.4:80442638-80442657 None:intergenic 35.0%
GAAGTGATTGTGCATGAAAT+GGG + chr8.4:80442741-80442760 MS.gene022560:CDS 35.0%
GACTTTGTAGTTCTTGATCA+TGG - chr8.4:80441749-80441768 None:intergenic 35.0%
GGACCAATTGAGATTAATTG+AGG - chr8.4:80441628-80441647 None:intergenic 35.0%
TCTAAATCCGTTAAATCAGC+TGG - chr8.4:80442366-80442385 None:intergenic 35.0%
TGGTCTTTGTAAACATGTTG+TGG + chr8.4:80442794-80442813 MS.gene022560:CDS 35.0%
! TTACCTTCCAGCTGATTTAA+CGG + chr8.4:80442356-80442375 MS.gene022560:CDS 35.0%
!! ACGTGAAACTAAGCTTGTTT+TGG + chr8.4:80442176-80442195 MS.gene022560:CDS 35.0%
!! CTGAGTTATTTTCTCTGCTT+AGG + chr8.4:80442507-80442526 MS.gene022560:CDS 35.0%
!! TGGTGTATTTTAGAGATGCT+TGG + chr8.4:80442904-80442923 MS.gene022560:CDS 35.0%
!! TTAATTGGTGATGTTGGTAG+TGG + chr8.4:80442237-80442256 MS.gene022560:CDS 35.0%
!!! CTTCTTTTTTGTCGTCGTTA+GGG - chr8.4:80442089-80442108 None:intergenic 35.0%
!!! GTGAGTTTTGAGAAAGTTGT+GGG + chr8.4:80442444-80442463 MS.gene022560:CDS 35.0%
!!! TCTTCTTTTTTGTCGTCGTT+AGG - chr8.4:80442090-80442109 None:intergenic 35.0%
!!! TGAGTTTTGAGAAAGTTGTG+GGG + chr8.4:80442445-80442464 MS.gene022560:CDS 35.0%
!!! TGTGAGTTTTGAGAAAGTTG+TGG + chr8.4:80442443-80442462 MS.gene022560:CDS 35.0%
AATCCGTTAAATCAGCTGGA+AGG - chr8.4:80442362-80442381 None:intergenic 40.0%
AGCAGATTGTAGCATCTACA+AGG + chr8.4:80441561-80441580 MS.gene022560:CDS 40.0%
GGAAGTGATTGTGCATGAAA+TGG + chr8.4:80442740-80442759 MS.gene022560:CDS 40.0%
TCAATTGGTCCAATTCACCT+TGG + chr8.4:80441637-80441656 MS.gene022560:CDS 40.0%
! CACAAGGGACTTGATTTTGT+TGG + chr8.4:80442014-80442033 MS.gene022560:CDS 40.0%
! GAATATTTGGAACCGTACCA+TGG + chr8.4:80442869-80442888 MS.gene022560:CDS 40.0%
! GTTTAGGACCTTGAAACCTA+TGG - chr8.4:80442311-80442330 None:intergenic 40.0%
!! AGAGCTAGTTCTACTTTGGT+TGG + chr8.4:80442927-80442946 MS.gene022560:CDS 40.0%
!! AGTGGCTTAATTGGTGATGT+TGG + chr8.4:80442231-80442250 MS.gene022560:CDS 40.0%
!! TGTTGATTTGTTGGTCGAGA+AGG + chr8.4:80442719-80442738 MS.gene022560:CDS 40.0%
!! TTCACTTTTCGGAGATTGTG+AGG - chr8.4:80441589-80441608 None:intergenic 40.0%
!! TTGGAGAGCTAGTTCTACTT+TGG + chr8.4:80442923-80442942 MS.gene022560:CDS 40.0%
!!! GTTGGATGCTGTTTTCATCA+TGG + chr8.4:80441858-80441877 MS.gene022560:CDS 40.0%
ACCATTTCAAAGCGCGTCTA+AGG + chr8.4:80442556-80442575 MS.gene022560:CDS 45.0%
GTGTGATCAACCTTCAACTG+CGG - chr8.4:80442585-80442604 None:intergenic 45.0%
TCTAAGGATTCCGCAGTTGA+AGG + chr8.4:80442572-80442591 MS.gene022560:CDS 45.0%
! GAAGTGGCAGTGCTTGTTAA+TGG + chr8.4:80442774-80442793 MS.gene022560:CDS 45.0%
!! GACATTGTTCTAGGGCAATG+AGG - chr8.4:80442629-80442648 None:intergenic 45.0%
!! GCTCAGCAAAAGCATCACAA+GGG + chr8.4:80441999-80442018 MS.gene022560:CDS 45.0%
!! TCCTTAGACGCGCTTTGAAA+TGG - chr8.4:80442560-80442579 None:intergenic 45.0%
ACCACAATGCAGCCATGGTA+CGG - chr8.4:80442884-80442903 None:intergenic 50.0%
TACCATGGCTGCATTGTGGT+TGG + chr8.4:80442884-80442903 MS.gene022560:CDS 50.0%
!! GGCTCAGCAAAAGCATCACA+AGG + chr8.4:80441998-80442017 MS.gene022560:CDS 50.0%
ACCGTACCATGGCTGCATTG+TGG + chr8.4:80442880-80442899 MS.gene022560:CDS 55.0%
CACCAACCACAATGCAGCCA+TGG - chr8.4:80442889-80442908 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 80441463 80443010 80441463 ID=MS.gene022560
chr8.4 mRNA 80441463 80443010 80441463 ID=MS.gene022560.t1;Parent=MS.gene022560
chr8.4 exon 80441463 80443010 80441463 ID=MS.gene022560.t1.exon1;Parent=MS.gene022560.t1
chr8.4 CDS 80441463 80443010 80441463 ID=cds.MS.gene022560.t1;Parent=MS.gene022560.t1
Gene Sequence

>MS.gene022560

ATGGCACATGCTAATACTTCTGTTGATGCTTTATCTGAAACTGAATATGAAATGGTGAAACACATCATTGACGTTGAAGTCCTTGAAGAGTTAAGTTTAGCAGATTGTAGCATCTACAAGGTTCCTCACAATCTCCGAAAAGTGAAACAAGAAGCTTACACTCCTCAATTAATCTCAATTGGTCCAATTCACCTTGGAAATAAAGAACTTCAACCAATGCAACAACACAAAAAAAGGTATTTCCATTTCTTCTATGAGCGTCTATCTCAATTCAATCAACAATCCATGATCAAGAACTACAAAGTCTATCTTGAAAACAAAGAACAAGAAATAAGAAAATGTTATGCAGAGAAATTTCCCAACATAAGCAAAGAAAAATTTGTGGAAACAATTTTGTTGGATGCTGTTTTCATCATGGAGCTTTTGTTAAGAAACCGTTCATGGAAATCGGATACATCAAAACATGAACATGAATACAAGCAATCCAAGTCATTTAGATTGAAACATAGTGATGATTACATTCTAACACAAGCTTGGCTCAGCAAAAGCATCACAAGGGACTTGATTTTGTTGGAAAATCAGATACCTTTTTTTGTGCTGATGAATCTTTACGATACTGTCGTCCCTAACGACGACAAAAAAGAAGACCATAAAAGTTTTCTTGATCTTGCACTTGAGTATTTTGCATTCTACGACACTCAGAAATCGTCATCACGTGAAACTAAGCTTGTTTTGGATAAGAATCAGACAAAGAAATATTATTTTGTGAGTGGCTTAATTGGTGATGTTGGTAGTGGTAATTCAGTTTCCAAGAAAAAACATTCTGATAAATCTATGAGCAATAACCATAGGTTTCAAGGTCCTAAACATTTCACTGATTTGATAAGGTTTTTTTACCTTCCAGCTGATTTAACGGATTTAGAAAAGAGTGGTTGTTCTCATACTTTTCCAAGAACAGTAACAAAGTTAATAGATTCTGGTGTGAGTTTTGAGAAAGTTGTGGGGAGAAGATTGCTAGAAATAACATTTGAGAAGAAACCATTTCTGAGTTATTTTCTCTGCTTAGGTTGTTTACCTTCATTTTCATGTTTGAACCATTTCAAAGCGCGTCTAAGGATTCCGCAGTTGAAGGTTGATCACACAACAGAATGTGTTTTAAGGAACCTCATTGCCCTAGAACAATGTCAGTATCAAGAACAACCTTATATATGCAATTATGTTTCACTTGTTGATTCTCTTATCCATACACAAGTTGATGTTGATTTGTTGGTCGAGAAGGAAGTGATTGTGCATGAAATGGGAAGTGATAAAGAAGTGGCAGTGCTTGTTAATGGTCTTTGTAAACATGTTGTGGTGAATTCAACTTGTTACTATGATATTATTAATGAGCTTAATAAGCATTATCAGAATATTTGGAACCGTACCATGGCTGCATTGTGGTTGGTGTATTTTAGAGATGCTTGGAGAGCTAGTTCTACTTTGGTTGGAATTGCTTTTCTTGTTTACACAGCATTCAACTTTGTACGTCTTGCTAGAATTCTCTTCTAA
Protein sequence

>MS.gene022560.t1

MAHANTSVDALSETEYEMVKHIIDVEVLEELSLADCSIYKVPHNLRKVKQEAYTPQLISIGPIHLGNKELQPMQQHKKRYFHFFYERLSQFNQQSMIKNYKVYLENKEQEIRKCYAEKFPNISKEKFVETILLDAVFIMELLLRNRSWKSDTSKHEHEYKQSKSFRLKHSDDYILTQAWLSKSITRDLILLENQIPFFVLMNLYDTVVPNDDKKEDHKSFLDLALEYFAFYDTQKSSSRETKLVLDKNQTKKYYFVSGLIGDVGSGNSVSKKKHSDKSMSNNHRFQGPKHFTDLIRFFYLPADLTDLEKSGCSHTFPRTVTKLIDSGVSFEKVVGRRLLEITFEKKPFLSYFLCLGCLPSFSCLNHFKARLRIPQLKVDHTTECVLRNLIALEQCQYQEQPYICNYVSLVDSLIHTQVDVDLLVEKEVIVHEMGSDKEVAVLVNGLCKHVVVNSTCYYDIINELNKHYQNIWNRTMAALWLVYFRDAWRASSTLVGIAFLVYTAFNFVRLARILF