AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022562

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022562.t1 MTR_8g005235 95.495 444 20 0 1 444 1 444 0.0 889
MS.gene022562.t1 MTR_8g005325 79.101 445 92 1 1 444 30 474 0.0 744
MS.gene022562.t1 MTR_8g006525 77.011 435 88 3 2 436 3 425 0.0 684
MS.gene022562.t1 MTR_0209s0060 76.092 435 88 4 3 436 5 424 0.0 672
MS.gene022562.t1 MTR_0209s0030 76.092 435 88 4 3 436 5 424 0.0 672
MS.gene022562.t1 MTR_8g005295 50.699 501 175 9 9 444 19 512 9.17e-163 469
MS.gene022562.t1 MTR_8g005285 51.753 485 180 9 9 444 19 498 1.48e-159 460
MS.gene022562.t1 MTR_8g005265 52.765 434 183 9 26 442 26 454 3.70e-150 435
MS.gene022562.t1 MTR_8g005225 50.679 442 202 6 12 442 22 458 6.07e-143 417
MS.gene022562.t1 MTR_4g112660 41.190 437 153 12 26 441 5 358 4.38e-93 285
MS.gene022562.t1 MTR_6g011050 33.488 430 241 13 3 426 4 394 2.98e-64 213
MS.gene022562.t1 MTR_0024s0080 34.074 405 222 12 28 426 26 391 3.98e-64 212
MS.gene022562.t1 MTR_6g009590 33.645 428 233 17 12 426 7 396 5.79e-64 212
MS.gene022562.t1 MTR_6g011060 32.960 446 245 14 4 432 2 410 2.65e-63 211
MS.gene022562.t1 MTR_3g107590 34.803 431 240 11 6 425 5 405 1.60e-61 206
MS.gene022562.t1 MTR_6g010920 32.464 422 239 13 21 434 19 402 4.25e-61 204
MS.gene022562.t1 MTR_6g010890 32.207 444 250 17 9 433 7 418 4.99e-61 204
MS.gene022562.t1 MTR_6g009610 34.033 429 230 17 9 426 10 396 1.16e-60 203
MS.gene022562.t1 MTR_3g079390 31.403 449 246 15 14 433 27 442 3.59e-58 197
MS.gene022562.t1 MTR_6g010810 32.787 427 239 14 17 433 12 400 3.39e-57 194
MS.gene022562.t1 MTR_6g009170 32.311 424 239 13 17 425 15 405 5.62e-57 194
MS.gene022562.t1 MTR_2g041180 29.561 433 266 15 11 434 39 441 1.67e-48 173
MS.gene022562.t1 MTR_2g036320 28.606 409 268 10 28 432 68 456 1.98e-45 165
MS.gene022562.t1 MTR_5g016570 27.253 455 252 12 12 417 79 503 2.54e-40 151
MS.gene022562.t1 MTR_6g010850 31.579 361 189 14 97 443 33 349 6.79e-39 144
MS.gene022562.t1 MTR_2g035640 30.769 377 216 10 64 432 1 340 8.02e-39 144
MS.gene022562.t1 MTR_5g086340 25.874 429 267 12 24 433 29 425 1.68e-34 134
MS.gene022562.t1 MTR_7g011980 34.389 221 116 6 222 434 136 335 1.92e-30 120
MS.gene022562.t1 MTR_7g011980 31.953 169 78 6 3 169 4 137 4.64e-15 76.6
MS.gene022562.t1 MTR_6g009580 34.597 211 111 5 221 425 30 219 3.08e-29 114
MS.gene022562.t1 MTR_8g042830 24.619 459 242 12 7 432 18 405 3.32e-27 113
MS.gene022562.t1 MTR_7g060840 25.219 456 283 16 3 434 5 426 7.84e-25 106
MS.gene022562.t1 MTR_7g060260 26.066 422 267 13 9 417 14 403 1.39e-24 105
MS.gene022562.t1 MTR_7g060310 23.506 502 284 13 3 432 6 479 3.77e-24 105
MS.gene022562.t1 MTR_7g060320 24.550 444 284 14 9 417 14 441 3.21e-22 99.0
MS.gene022562.t1 MTR_7g446270 24.678 466 280 16 10 417 9 461 9.37e-22 97.8
MS.gene022562.t1 MTR_7g059540 23.810 483 285 14 3 432 6 458 2.50e-21 96.7
MS.gene022562.t1 MTR_7g059540 23.471 507 267 16 3 432 5 467 7.25e-21 95.1
MS.gene022562.t1 MTR_6g009600 36.129 155 74 6 244 394 17 150 8.62e-21 89.0
MS.gene022562.t1 MTR_5g087580 28.495 186 112 2 249 433 42 207 2.23e-20 89.7
MS.gene022562.t1 MTR_7g060115 26.010 396 233 15 41 398 41 414 2.38e-20 93.6
MS.gene022562.t1 MTR_7g060020 25.163 461 268 20 10 434 9 428 2.50e-20 93.2
MS.gene022562.t1 MTR_5g086230 33.333 183 104 5 1 173 7 181 2.76e-19 86.3
MS.gene022562.t1 MTR_8g042170 25.339 442 274 14 29 432 26 449 3.55e-19 90.1
MS.gene022562.t1 MTR_7g059510 24.454 458 249 15 12 434 20 415 5.43e-19 89.0
MS.gene022562.t1 MTR_7g059520 28.889 180 106 4 250 428 5 163 2.63e-18 82.8
MS.gene022562.t1 MTR_7g059810 25.185 405 250 11 41 417 42 421 2.68e-18 87.4
MS.gene022562.t1 MTR_7g060220 24.874 398 247 11 44 417 44 413 4.41e-18 86.7
MS.gene022562.t1 MTR_8g037923 25.000 456 266 17 29 432 31 462 2.15e-17 84.7
MS.gene022562.t1 MTR_7g060370 24.711 433 243 15 41 417 42 447 3.43e-17 84.0
MS.gene022562.t1 MTR_7g060080 28.365 208 126 4 233 435 1 190 5.16e-17 79.7
MS.gene022562.t1 MTR_5g086260 29.381 194 115 4 250 441 136 309 6.59e-17 81.6
MS.gene022562.t1 MTR_7g059525 25.926 189 123 2 245 432 103 275 1.88e-16 79.7
MS.gene022562.t1 MTR_7g060160 26.178 382 200 15 41 397 41 365 1.91e-16 81.3
MS.gene022562.t1 MTR_8g037920 24.703 421 255 16 48 432 7 401 2.00e-16 81.3
MS.gene022562.t1 MTR_0182s0060 29.891 184 111 4 250 432 342 508 7.18e-16 80.1
MS.gene022562.t1 MTR_7g059480 29.577 142 99 1 292 432 624 765 4.99e-15 77.8
MS.gene022562.t1 MTR_3g107600 27.358 212 102 6 221 426 406 571 5.40e-15 77.8
MS.gene022562.t1 MTR_4g009340 31.677 161 94 6 27 175 42 198 7.70e-14 73.9
MS.gene022562.t1 MTR_4g009340 26.286 175 109 3 245 418 373 528 2.46e-11 66.2
MS.gene022562.t1 MTR_4g009590 27.523 218 139 7 27 232 42 252 3.90e-13 71.6
MS.gene022562.t1 MTR_4g009620 32.919 161 91 7 27 175 42 197 4.77e-13 71.6
MS.gene022562.t1 MTR_3g466740 25.258 388 228 15 29 379 32 394 1.18e-12 70.1
MS.gene022562.t1 MTR_7g017620 30.534 131 89 2 302 431 401 530 2.84e-12 68.9
MS.gene022562.t1 MTR_0067s0010 24.173 393 220 16 29 379 45 401 6.62e-12 67.8
MS.gene022562.t1 MTR_2g036310 31.061 132 89 1 303 432 19 150 1.78e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022562.t1 AT2G28580 33.949 433 227 12 27 433 41 440 6.17e-65 216
MS.gene022562.t1 AT2G28580 33.949 433 227 12 27 433 123 522 2.48e-64 216
MS.gene022562.t1 AT2G28580 33.871 434 228 12 27 434 109 509 2.74e-64 216
MS.gene022562.t1 AT2G44930 33.482 448 230 12 24 435 75 490 1.25e-63 214
MS.gene022562.t1 AT2G28580 33.729 421 227 12 27 433 109 491 2.68e-62 210
MS.gene022562.t1 AT2G28580 33.729 421 227 12 27 433 123 505 4.01e-62 210
MS.gene022562.t1 AT1G67150 29.833 419 251 7 28 433 7 395 2.20e-53 184
MS.gene022562.t1 AT1G67150 29.833 419 251 7 28 433 7 395 2.20e-53 184
MS.gene022562.t1 AT1G67150 29.551 423 251 7 28 433 7 399 7.64e-53 182
MS.gene022562.t1 AT3G50150 28.043 460 268 13 4 434 70 495 3.03e-48 172
MS.gene022562.t1 AT4G31980 29.177 401 247 11 26 422 292 659 3.41e-48 175
MS.gene022562.t1 AT3G50160 28.471 425 252 9 22 432 99 485 1.54e-46 168
MS.gene022562.t1 AT1G67150 28.230 418 216 9 28 433 7 352 3.27e-46 164
MS.gene022562.t1 AT3G50170 26.797 459 280 9 4 433 93 524 8.06e-46 167
MS.gene022562.t1 AT5G11290 26.714 423 277 9 14 432 18 411 1.00e-45 164
MS.gene022562.t1 AT3G50140 26.856 458 281 11 5 434 94 525 2.05e-45 166
MS.gene022562.t1 AT2G36430 28.288 403 253 12 25 417 43 419 7.48e-44 159
MS.gene022562.t1 AT1G65985 30.626 431 238 12 28 417 15 425 8.32e-44 159
MS.gene022562.t1 AT1G65985 30.626 431 238 12 28 417 15 425 8.32e-44 159
MS.gene022562.t1 AT3G50120 28.043 460 267 14 12 432 81 515 1.69e-43 160
MS.gene022562.t1 AT2G28580 33.520 358 179 12 27 358 123 447 2.59e-43 159
MS.gene022562.t1 AT3G47210 30.521 403 222 13 19 401 85 449 1.36e-42 157
MS.gene022562.t1 AT3G50180 27.902 448 271 12 4 432 158 572 2.44e-41 155
MS.gene022562.t1 AT5G22540 27.845 413 240 12 27 417 31 407 2.44e-40 150
MS.gene022562.t1 AT3G50130 28.090 445 259 14 4 417 119 533 6.13e-40 151
MS.gene022562.t1 AT5G22550 26.949 449 271 13 27 434 44 476 8.55e-38 144
MS.gene022562.t1 AT3G47250 25.169 445 271 11 19 433 53 465 1.85e-37 142
MS.gene022562.t1 AT3G47250 25.169 445 271 11 19 433 53 465 1.85e-37 142
MS.gene022562.t1 AT3G47250 25.169 445 271 11 19 433 53 465 1.85e-37 142
MS.gene022562.t1 AT3G47200 26.185 443 269 13 19 434 38 449 9.94e-37 140
MS.gene022562.t1 AT3G47200 26.185 443 269 13 19 434 38 449 9.94e-37 140
MS.gene022562.t1 AT3G50190 26.386 451 259 13 5 432 77 477 2.75e-35 137
MS.gene022562.t1 AT5G11290 25.664 339 223 7 96 432 31 342 3.79e-31 123
MS.gene022562.t1 AT3G44710 24.672 458 293 14 20 433 40 489 1.52e-28 118
MS.gene022562.t1 AT3G60470 34.034 238 108 7 21 227 26 245 1.56e-26 112
MS.gene022562.t1 AT3G60470 29.508 244 130 7 199 433 317 527 2.66e-23 102
MS.gene022562.t1 AT5G22550 24.664 446 236 15 27 434 44 427 2.08e-25 108
MS.gene022562.t1 AT3G50150 25.485 361 217 12 4 326 70 416 9.09e-24 103
MS.gene022562.t1 AT5G22560 28.194 227 138 5 27 236 47 265 3.29e-22 99.4
MS.gene022562.t1 AT5G22560 28.358 134 94 1 303 434 370 503 8.39e-12 67.4
MS.gene022562.t1 AT3G50170 25.000 300 191 7 4 276 93 385 4.02e-22 98.6
MS.gene022562.t1 AT3G50140 25.503 298 190 8 5 276 94 385 1.00e-21 97.1
MS.gene022562.t1 AT3G50190 25.086 291 168 9 5 276 77 336 1.01e-17 84.7
MS.gene022562.t1 AT3G50190 25.685 292 165 10 5 276 77 336 1.30e-17 84.7
MS.gene022562.t1 AT3G50190 25.685 292 165 10 5 276 77 336 1.30e-17 84.7
MS.gene022562.t1 AT3G02645 27.429 175 108 2 245 418 341 497 3.36e-16 81.3
MS.gene022562.t1 AT3G44700 32.258 124 78 5 14 131 34 157 6.56e-13 68.2

Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGCAGTTCTACCATAAT+TGG 0.193723 8.4:-80407778 MS.gene022562:CDS
ATAGATATTTCCATTACTTT+TGG 0.255996 8.4:-80408824 MS.gene022562:CDS
ATTGGTTGACATGATACTAT+TGG 0.305573 8.4:-80408688 MS.gene022562:CDS
TTTAACTTTCGTCGCGTTGT+TGG 0.311790 8.4:-80407730 MS.gene022562:CDS
TGATATCTGCGAATTTCTTT+TGG 0.336636 8.4:+80408721 None:intergenic
TAGAACAGCTATCCCAATTA+TGG 0.339485 8.4:+80407765 None:intergenic
AGAAGCAATTGTGCACGAAT+TGG 0.353119 8.4:-80407965 MS.gene022562:CDS
TTGAGTTGGTTTCTTTGTTT+AGG 0.358010 8.4:-80408189 MS.gene022562:CDS
GTCGTGTTATTACCAACTTA+TGG 0.367785 8.4:-80407881 MS.gene022562:CDS
ACAAAGAATGTTTGATGGTA+AGG 0.389012 8.4:-80408302 MS.gene022562:CDS
ACACCTCTACTCATATCTAT+TGG 0.391628 8.4:-80408897 MS.gene022562:CDS
CTGGGATGTTAATTATTTGT+TGG 0.398663 8.4:+80409003 None:intergenic
GAAAGTTTGAGGCAAAGAAA+TGG 0.405649 8.4:-80408404 MS.gene022562:CDS
AAAGTTTGAGGCAAAGAAAT+GGG 0.406405 8.4:-80408403 MS.gene022562:CDS
TTGCAAATGTAAGGCTCATC+AGG 0.412545 8.4:+80408051 None:intergenic
TAGCAACTCTGCGTCTTCTT+CGG 0.424083 8.4:+80407996 None:intergenic
GAAGCAATTGTGCACGAATT+GGG 0.427029 8.4:-80407964 MS.gene022562:CDS
CATTAAGATCTTCCATAAGT+TGG 0.441137 8.4:+80407869 None:intergenic
AGATATCAACAATGAACAAT+TGG 0.443215 8.4:-80408706 MS.gene022562:CDS
GAAACATAATTGCAAATGTA+AGG 0.443461 8.4:+80408042 None:intergenic
TTCACCTTCAAAAGAGTAGC+AGG 0.450633 8.4:+80408933 None:intergenic
CCATAACAACAAAAGACTTA+AGG 0.457511 8.4:-80408865 MS.gene022562:CDS
CATCTTGTTACCCGCATCAC+AGG 0.462910 8.4:-80408440 MS.gene022562:CDS
ACTACAACAGTGAATGGAAA+AGG 0.467565 8.4:-80407843 MS.gene022562:CDS
ATTGGATTCTGTGTTCATCA+TGG 0.468580 8.4:-80408670 MS.gene022562:CDS
TCAGCTTCCGATTTATGTGT+TGG 0.469424 8.4:-80408541 MS.gene022562:CDS
ATGAACACTACAACAGTGAA+TGG 0.475271 8.4:-80407849 MS.gene022562:CDS
CTGAAGATTCCCTGTGATGC+GGG 0.477114 8.4:+80408430 None:intergenic
CAGTGAATGGAAAAGGGCTA+TGG 0.481943 8.4:-80407836 MS.gene022562:CDS
CTCAAACTTTATGTCAAGCA+AGG 0.488762 8.4:+80408224 None:intergenic
GAAGCAGTTCTACCATAATT+GGG 0.495113 8.4:-80407777 MS.gene022562:CDS
GACACTGCTCAAAAGCGATT+AGG 0.499699 8.4:+80408079 None:intergenic
GATACAACATTGAGGCCAAT+GGG 0.502608 8.4:+80408965 None:intergenic
CTACAACAGTGAATGGAAAA+GGG 0.513538 8.4:-80407842 MS.gene022562:CDS
GCAACAAAGTTGAATGAAGC+TGG 0.514234 8.4:-80408276 MS.gene022562:CDS
GGGCTATGGGTACATTAAAG+TGG 0.515344 8.4:-80407822 MS.gene022562:CDS
GCCTCAATGTTGTATCTACA+AGG 0.517588 8.4:-80408958 MS.gene022562:CDS
TAGGTTCCTCAAAACACATT+CGG 0.523556 8.4:+80408098 None:intergenic
GGCTATGGGTACATTAAAGT+GGG 0.534765 8.4:-80407821 MS.gene022562:CDS
CTAGGCGATTCCAAAAGTAA+TGG 0.536662 8.4:+80408814 None:intergenic
TCTGAAGATTCCCTGTGATG+CGG 0.546993 8.4:+80408429 None:intergenic
ATCTTCAGAAAGAAAGTTTG+AGG 0.554166 8.4:-80408415 MS.gene022562:CDS
AGATACAACATTGAGGCCAA+TGG 0.562113 8.4:+80408964 None:intergenic
AGCTGGTATAAGCTTTGAGA+AGG 0.574187 8.4:-80408259 MS.gene022562:CDS
GTTTGCACAGATTGTTAACA+AGG 0.589492 8.4:+80407920 None:intergenic
AGTGAATGGAAAAGGGCTAT+GGG 0.589763 8.4:-80407835 MS.gene022562:CDS
AAAATATAGAGCCTTCCCAT+TGG 0.601300 8.4:-80408980 MS.gene022562:CDS
ATCTTGTTACCCGCATCACA+GGG 0.604095 8.4:-80408439 MS.gene022562:CDS
CAACATTGAGGCCAATGGGA+AGG 0.610470 8.4:+80408969 None:intergenic
CAAGAAGCAATAAATCACGC+TGG 0.619840 8.4:+80408568 None:intergenic
ATGGTAGAACTGCTTCTCCA+AGG 0.620610 8.4:+80407784 None:intergenic
CAAAGAAACCAACTCAACAA+AGG 0.633809 8.4:+80408195 None:intergenic
GGTCCAATAGATATGAGTAG+AGG 0.668562 8.4:+80408894 None:intergenic
GCTTTGAAGCACTTGCAACA+CGG 0.686234 8.4:+80408159 None:intergenic
GCACGAATTGGGAAGCAACG+AGG 0.701700 8.4:-80407953 MS.gene022562:CDS
ACCTTGTAGATACAACATTG+AGG 0.707204 8.4:+80408957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAAGAGAAAACAAAAAAT+GGG - chr8.4:80408106-80408125 MS.gene022562:CDS 20.0%
!!! ACTGATTTTTTAGTTTCATA+GGG + chr8.4:80408412-80408431 None:intergenic 20.0%
!!! ATAGATATTTCCATTACTTT+TGG - chr8.4:80407922-80407941 MS.gene022562:CDS 20.0%
!!! TACTGATTTTTTAGTTTCAT+AGG + chr8.4:80408413-80408432 None:intergenic 20.0%
!!! TTCTAAGAGAAAACAAAAAA+TGG - chr8.4:80408105-80408124 MS.gene022562:CDS 20.0%
! AGATATCAACAATGAACAAT+TGG - chr8.4:80408040-80408059 MS.gene022562:CDS 25.0%
! GAAACATAATTGCAAATGTA+AGG + chr8.4:80408707-80408726 None:intergenic 25.0%
!! AGTTTTTCCAACACATAAAT+CGG + chr8.4:80408215-80408234 None:intergenic 25.0%
!! TTTTCACAAAGAATGTTTGA+TGG - chr8.4:80408439-80408458 MS.gene022562:CDS 25.0%
!!! TGAAATCACTTTTTTTGTCT+AGG + chr8.4:80407953-80407972 None:intergenic 25.0%
AGAAAAAACCTTTGTTGAGT+TGG - chr8.4:80408543-80408562 MS.gene022562:CDS 30.0%
ATCTTCAGAAAGAAAGTTTG+AGG - chr8.4:80408331-80408350 MS.gene022562:CDS 30.0%
ATTGGTTGACATGATACTAT+TGG - chr8.4:80408058-80408077 MS.gene022562:CDS 30.0%
CATTAAGATCTTCCATAAGT+TGG + chr8.4:80408880-80408899 None:intergenic 30.0%
CCATAACAACAAAAGACTTA+AGG - chr8.4:80407881-80407900 MS.gene022562:CDS 30.0%
CTGGGATGTTAATTATTTGT+TGG + chr8.4:80407746-80407765 None:intergenic 30.0%
! AAAGTTTGAGGCAAAGAAAT+GGG - chr8.4:80408343-80408362 MS.gene022562:CDS 30.0%
! ACAAAGAATGTTTGATGGTA+AGG - chr8.4:80408444-80408463 MS.gene022562:CDS 30.0%
! TATTTTGCTCTTCTTGTTGA+AGG + chr8.4:80407992-80408011 None:intergenic 30.0%
! TGATATCTGCGAATTTCTTT+TGG + chr8.4:80408028-80408047 None:intergenic 30.0%
!! CGAATTTCTTTTGGTAACAT+CGG + chr8.4:80408019-80408038 None:intergenic 30.0%
!! TTGAGTTGGTTTCTTTGTTT+AGG - chr8.4:80408557-80408576 MS.gene022562:CDS 30.0%
!!! CCTTAAGTCTTTTGTTGTTA+TGG + chr8.4:80407884-80407903 None:intergenic 30.0%
!!! TAAGTCTTTTGTTGTTATGG+TGG + chr8.4:80407881-80407900 None:intergenic 30.0%
AAAATATAGAGCCTTCCCAT+TGG - chr8.4:80407766-80407785 MS.gene022562:CDS 35.0%
ACACCTCTACTCATATCTAT+TGG - chr8.4:80407849-80407868 MS.gene022562:CDS 35.0%
ACCTTGTAGATACAACATTG+AGG + chr8.4:80407792-80407811 None:intergenic 35.0%
ACTACAACAGTGAATGGAAA+AGG - chr8.4:80408903-80408922 MS.gene022562:CDS 35.0%
AGAAGCAGTTCTACCATAAT+TGG - chr8.4:80408968-80408987 MS.gene022562:CDS 35.0%
ATGAACACTACAACAGTGAA+TGG - chr8.4:80408897-80408916 MS.gene022562:CDS 35.0%
ATTGGATTCTGTGTTCATCA+TGG - chr8.4:80408076-80408095 MS.gene022562:CDS 35.0%
CAAAGAAACCAACTCAACAA+AGG + chr8.4:80408554-80408573 None:intergenic 35.0%
CTACAACAGTGAATGGAAAA+GGG - chr8.4:80408904-80408923 MS.gene022562:CDS 35.0%
CTCAAACTTTATGTCAAGCA+AGG + chr8.4:80408525-80408544 None:intergenic 35.0%
GAAGCAGTTCTACCATAATT+GGG - chr8.4:80408969-80408988 MS.gene022562:CDS 35.0%
GTTTGCACAGATTGTTAACA+AGG + chr8.4:80408829-80408848 None:intergenic 35.0%
TAGAACAGCTATCCCAATTA+TGG + chr8.4:80408984-80409003 None:intergenic 35.0%
TAGGTTCCTCAAAACACATT+CGG + chr8.4:80408651-80408670 None:intergenic 35.0%
! GAAAGTTTGAGGCAAAGAAA+TGG - chr8.4:80408342-80408361 MS.gene022562:CDS 35.0%
! GCAATGTCAAAAAGCACAAT+TGG - chr8.4:80408255-80408274 MS.gene022562:CDS 35.0%
! GTCGTGTTATTACCAACTTA+TGG - chr8.4:80408865-80408884 MS.gene022562:CDS 35.0%
!! CGTGATCTACTTTTAACTGT+GGG + chr8.4:80408625-80408644 None:intergenic 35.0%
!! TACTCTTTTGAAGGTGAAAG+AGG - chr8.4:80407818-80407837 MS.gene022562:CDS 35.0%
!! TTGTGCTTTTTGACATTGCT+TGG + chr8.4:80408254-80408273 None:intergenic 35.0%
!!! CTTTTGTTGTTATGGTGGAT+AGG + chr8.4:80407876-80407895 None:intergenic 35.0%
AGAAGCAATTGTGCACGAAT+TGG - chr8.4:80408781-80408800 MS.gene022562:CDS 40.0%
AGATACAACATTGAGGCCAA+TGG + chr8.4:80407785-80407804 None:intergenic 40.0%
AGTGAATGGAAAAGGGCTAT+GGG - chr8.4:80408911-80408930 MS.gene022562:CDS 40.0%
CAAGAAGCAATAAATCACGC+TGG + chr8.4:80408181-80408200 None:intergenic 40.0%
CTAGGCGATTCCAAAAGTAA+TGG + chr8.4:80407935-80407954 None:intergenic 40.0%
GAAGCAATTGTGCACGAATT+GGG - chr8.4:80408782-80408801 MS.gene022562:CDS 40.0%
GATACAACATTGAGGCCAAT+GGG + chr8.4:80407784-80407803 None:intergenic 40.0%
GCAACAAAGTTGAATGAAGC+TGG - chr8.4:80408470-80408489 MS.gene022562:CDS 40.0%
GCCTCAATGTTGTATCTACA+AGG - chr8.4:80407788-80407807 MS.gene022562:CDS 40.0%
GGCTATGGGTACATTAAAGT+GGG - chr8.4:80408925-80408944 MS.gene022562:CDS 40.0%
GGTCCAATAGATATGAGTAG+AGG + chr8.4:80407855-80407874 None:intergenic 40.0%
TCAGCTTCCGATTTATGTGT+TGG - chr8.4:80408205-80408224 MS.gene022562:CDS 40.0%
TTCACCTTCAAAAGAGTAGC+AGG + chr8.4:80407816-80407835 None:intergenic 40.0%
TTGCAAATGTAAGGCTCATC+AGG + chr8.4:80408698-80408717 None:intergenic 40.0%
! GGGAAGGCTCTATATTTTCT+GGG + chr8.4:80407764-80407783 None:intergenic 40.0%
! TGGGAAGGCTCTATATTTTC+TGG + chr8.4:80407765-80407784 None:intergenic 40.0%
! TTTAACTTTCGTCGCGTTGT+TGG - chr8.4:80409016-80409035 MS.gene022562:CDS 40.0%
!! AGCTGGTATAAGCTTTGAGA+AGG - chr8.4:80408487-80408506 MS.gene022562:CDS 40.0%
!! GCGTGATCTACTTTTAACTG+TGG + chr8.4:80408626-80408645 None:intergenic 40.0%
!! GGGTTTATTTTCGTGATCCT+TGG - chr8.4:80408945-80408964 MS.gene022562:CDS 40.0%
!! TCTTTTGAAGGTGAAAGAGG+AGG - chr8.4:80407821-80407840 MS.gene022562:CDS 40.0%
ATCTTGTTACCCGCATCACA+GGG - chr8.4:80408307-80408326 MS.gene022562:CDS 45.0%
ATGGTAGAACTGCTTCTCCA+AGG + chr8.4:80408965-80408984 None:intergenic 45.0%
CAGTGAATGGAAAAGGGCTA+TGG - chr8.4:80408910-80408929 MS.gene022562:CDS 45.0%
GACACTGCTCAAAAGCGATT+AGG + chr8.4:80408670-80408689 None:intergenic 45.0%
GGGCTATGGGTACATTAAAG+TGG - chr8.4:80408924-80408943 MS.gene022562:CDS 45.0%
TAGCAACTCTGCGTCTTCTT+CGG + chr8.4:80408753-80408772 None:intergenic 45.0%
TCTGAAGATTCCCTGTGATG+CGG + chr8.4:80408320-80408339 None:intergenic 45.0%
! GGTTCCTGCTACTCTTTTGA+AGG - chr8.4:80407809-80407828 MS.gene022562:CDS 45.0%
!! GCTTTGAAGCACTTGCAACA+CGG + chr8.4:80408590-80408609 None:intergenic 45.0%
CAACATTGAGGCCAATGGGA+AGG + chr8.4:80407780-80407799 None:intergenic 50.0%
CATCTTGTTACCCGCATCAC+AGG - chr8.4:80408306-80408325 MS.gene022562:CDS 50.0%
CTGAAGATTCCCTGTGATGC+GGG + chr8.4:80408319-80408338 None:intergenic 50.0%
! ACGCGACCGAATGTGTTTTG+AGG - chr8.4:80408642-80408661 MS.gene022562:CDS 50.0%
GCACGAATTGGGAAGCAACG+AGG - chr8.4:80408793-80408812 MS.gene022562:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 80407717 80409051 80407717 ID=MS.gene022562
chr8.4 mRNA 80407717 80409051 80407717 ID=MS.gene022562.t1;Parent=MS.gene022562
chr8.4 exon 80407717 80409051 80407717 ID=MS.gene022562.t1.exon1;Parent=MS.gene022562.t1
chr8.4 CDS 80407717 80409051 80407717 ID=cds.MS.gene022562.t1;Parent=MS.gene022562.t1
Gene Sequence

>MS.gene022562

ATGGGATCTTCTATTGAAGATCATATCCAACAAATAATTAACATCCCAGAAAATATAGAGCCTTCCCATTGGCCTCAATGTTGTATCTACAAGGTTCCTGCTACTCTTTTGAAGGTGAAAGAGGAGGCTTATACACCTCTACTCATATCTATTGGACCTATCCACCATAACAACAAAAGACTTAAGGAATTGCAAGAGTACAAACATAGATATTTCCATTACTTTTGGAATCGCCTAGACAAAAAAAGTGATTTCATGAGCTACAAAGTTTTCCTTCAACAAGAAGAGCAAAATATACGCCGATGTTACCAAAAGAAATTCGCAGATATCAACAATGAACAATTGGTTGACATGATACTATTGGATTCTGTGTTCATCATGGAGTTGTTTCTAAGAGAAAACAAAAAATGGGAGCACAAAGATGATTATATAATAACTCAACTTTGTGTAAGTAAAAGTATCCAGCGTGATTTATTGCTTCTTGAGAATCAGCTTCCGATTTATGTGTTGGAAAAACTATATGACACAATTGTTCCAAGCAATGTCAAAAAGCACAATTGGTTTCTCAAGCTTGCACATGAGTATTTTGCATCTTGTTACCCGCATCACAGGGAATCTTCAGAAAGAAAGTTTGAGGCAAAGAAATGGGAGAAATCACGTCATTTCACTGATTTGATTAGATATTCGTATCTCCCTATGAAACTAAAAAATCAGTATAATGATTTTCACAAAGAATGTTTGATGGTAAGGACTGCAACAAAGTTGAATGAAGCTGGTATAAGCTTTGAGAAGGTTCATAACAGATCCTTGCTTGACATAAAGTTTGAGAAAAAACCTTTGTTGAGTTGGTTTCTTTGTTTAGGTTGTTTACCGTGTTGCAAGTGCTTCAAAGCTAGATTTCTATTCCCACAGTTAAAAGTAGATCACGCGACCGAATGTGTTTTGAGGAACCTAATCGCTTTTGAGCAGTGTCACTATCCTGATGAGCCTTACATTTGCAATTATGTTTCTTTGATTGATTCTCTTATTCACACCGAAGAAGACGCAGAGTTGCTAGTTGAGAAAGAAGCAATTGTGCACGAATTGGGAAGCAACGAGGAATTAACGAGCCTTGTTAACAATCTGTGCAAACATGTTGTGACAAATTCGTCGTGTTATTACCAACTTATGGAAGATCTTAATGAACACTACAACAGTGAATGGAAAAGGGCTATGGGTACATTAAAGTGGGTTTATTTTCGTGATCCTTGGAGAAGCAGTTCTACCATAATTGGGATAGCTGTTCTAATATTCACTATCTTTAACTTTCGTCGCGTTGTTGGTCTAATGTTTTAG
Protein sequence

>MS.gene022562.t1

MGSSIEDHIQQIINIPENIEPSHWPQCCIYKVPATLLKVKEEAYTPLLISIGPIHHNNKRLKELQEYKHRYFHYFWNRLDKKSDFMSYKVFLQQEEQNIRRCYQKKFADINNEQLVDMILLDSVFIMELFLRENKKWEHKDDYIITQLCVSKSIQRDLLLLENQLPIYVLEKLYDTIVPSNVKKHNWFLKLAHEYFASCYPHHRESSERKFEAKKWEKSRHFTDLIRYSYLPMKLKNQYNDFHKECLMVRTATKLNEAGISFEKVHNRSLLDIKFEKKPLLSWFLCLGCLPCCKCFKARFLFPQLKVDHATECVLRNLIAFEQCHYPDEPYICNYVSLIDSLIHTEEDAELLVEKEAIVHELGSNEELTSLVNNLCKHVVTNSSCYYQLMEDLNEHYNSEWKRAMGTLKWVYFRDPWRSSSTIIGIAVLIFTIFNFRRVVGLMF