AlfalfaGEDB | MS.gene022562

M. sativa cultivar XinJiangDaYe / MS.gene022562

KEGG: -
GO: -
PFAM: PF03140.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022562.t1	XP_013443753.1	95.5	444	20	0	1	444	1	444	1.20E-254	888.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022562.t1	Q9SD53	26.1	441	270	11	19	433	38	448	2.6e-29	131.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022562.t1	A0A072TLP8	95.5	444	20	0	1	444	1	444	8.3e-255	888.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene019468	MS.gene022562	PPI
MS.gene022559	MS.gene022562	PPI
MS.gene022561	MS.gene022562	PPI
MS.gene022562	MS.gene91808	PPI
MS.gene022562	MS.gene055359	PPI
MS.gene022562	MS.gene022560	PPI
MS.gene022562	MS.gene91809	PPI
MS.gene022562	MS.gene061335	PPI
MS.gene022562	MS.gene061334	PPI
MS.gene022562	MS.gene055357	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022562.t1	MTR_8g005235	95.495	444	20	0	1	444	1	444	0.0	889
MS.gene022562.t1	MTR_8g005325	79.101	445	92	1	1	444	30	474	0.0	744
MS.gene022562.t1	MTR_8g006525	77.011	435	88	3	2	436	3	425	0.0	684
MS.gene022562.t1	MTR_0209s0060	76.092	435	88	4	3	436	5	424	0.0	672
MS.gene022562.t1	MTR_0209s0030	76.092	435	88	4	3	436	5	424	0.0	672
MS.gene022562.t1	MTR_8g005295	50.699	501	175	9	9	444	19	512	9.17e-163	469
MS.gene022562.t1	MTR_8g005285	51.753	485	180	9	9	444	19	498	1.48e-159	460
MS.gene022562.t1	MTR_8g005265	52.765	434	183	9	26	442	26	454	3.70e-150	435
MS.gene022562.t1	MTR_8g005225	50.679	442	202	6	12	442	22	458	6.07e-143	417
MS.gene022562.t1	MTR_4g112660	41.190	437	153	12	26	441	5	358	4.38e-93	285

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022562.t1	AT2G28580	33.949	433	227	12	27	433	41	440	6.17e-65	216
MS.gene022562.t1	AT2G28580	33.949	433	227	12	27	433	123	522	2.48e-64	216
MS.gene022562.t1	AT2G28580	33.871	434	228	12	27	434	109	509	2.74e-64	216
MS.gene022562.t1	AT2G44930	33.482	448	230	12	24	435	75	490	1.25e-63	214
MS.gene022562.t1	AT2G28580	33.729	421	227	12	27	433	109	491	2.68e-62	210
MS.gene022562.t1	AT2G28580	33.729	421	227	12	27	433	123	505	4.01e-62	210
MS.gene022562.t1	AT1G67150	29.833	419	251	7	28	433	7	395	2.20e-53	184
MS.gene022562.t1	AT1G67150	29.833	419	251	7	28	433	7	395	2.20e-53	184
MS.gene022562.t1	AT1G67150	29.551	423	251	7	28	433	7	399	7.64e-53	182
MS.gene022562.t1	AT3G50150	28.043	460	268	13	4	434	70	495	3.03e-48	172

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Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATAGAGCCTTCCCAT+TGG	0.601300	8.4:-80408980	MS.gene022562:CDS
AAAGTTTGAGGCAAAGAAAT+GGG	0.406405	8.4:-80408403	MS.gene022562:CDS
ACAAAGAATGTTTGATGGTA+AGG	0.389012	8.4:-80408302	MS.gene022562:CDS
ACACCTCTACTCATATCTAT+TGG	0.391628	8.4:-80408897	MS.gene022562:CDS
ACCTTGTAGATACAACATTG+AGG	0.707204	8.4:+80408957	None:intergenic
ACTACAACAGTGAATGGAAA+AGG	0.467565	8.4:-80407843	MS.gene022562:CDS
AGAAGCAATTGTGCACGAAT+TGG	0.353119	8.4:-80407965	MS.gene022562:CDS
AGAAGCAGTTCTACCATAAT+TGG	0.193723	8.4:-80407778	MS.gene022562:CDS
AGATACAACATTGAGGCCAA+TGG	0.562113	8.4:+80408964	None:intergenic
AGATATCAACAATGAACAAT+TGG	0.443215	8.4:-80408706	MS.gene022562:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAAAAACCTTTGTTGAGT+TGG	-	chr8.4:80408543-80408562	MS.gene022562:CDS	30.0%
ATCTTCAGAAAGAAAGTTTG+AGG	-	chr8.4:80408331-80408350	MS.gene022562:CDS	30.0%
ATTGGTTGACATGATACTAT+TGG	-	chr8.4:80408058-80408077	MS.gene022562:CDS	30.0%
CATTAAGATCTTCCATAAGT+TGG	+	chr8.4:80408880-80408899	None:intergenic	30.0%
CCATAACAACAAAAGACTTA+AGG	-	chr8.4:80407881-80407900	MS.gene022562:CDS	30.0%
CTGGGATGTTAATTATTTGT+TGG	+	chr8.4:80407746-80407765	None:intergenic	30.0%
AAAATATAGAGCCTTCCCAT+TGG	-	chr8.4:80407766-80407785	MS.gene022562:CDS	35.0%
ACACCTCTACTCATATCTAT+TGG	-	chr8.4:80407849-80407868	MS.gene022562:CDS	35.0%
ACCTTGTAGATACAACATTG+AGG	+	chr8.4:80407792-80407811	None:intergenic	35.0%
ACTACAACAGTGAATGGAAA+AGG	-	chr8.4:80408903-80408922	MS.gene022562:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	80407717	80409051	80407717	ID=MS.gene022562
chr8.4	mRNA	80407717	80409051	80407717	ID=MS.gene022562.t1;Parent=MS.gene022562
chr8.4	exon	80407717	80409051	80407717	ID=MS.gene022562.t1.exon1;Parent=MS.gene022562.t1
chr8.4	CDS	80407717	80409051	80407717	ID=cds.MS.gene022562.t1;Parent=MS.gene022562.t1

Gene Sequence

>MS.gene022562

ATGGGATCTTCTATTGAAGATCATATCCAACAAATAATTAACATCCCAGAAAATATAGAGCCTTCCCATTGGCCTCAATGTTGTATCTACAAGGTTCCTGCTACTCTTTTGAAGGTGAAAGAGGAGGCTTATACACCTCTACTCATATCTATTGGACCTATCCACCATAACAACAAAAGACTTAAGGAATTGCAAGAGTACAAACATAGATATTTCCATTACTTTTGGAATCGCCTAGACAAAAAAAGTGATTTCATGAGCTACAAAGTTTTCCTTCAACAAGAAGAGCAAAATATACGCCGATGTTACCAAAAGAAATTCGCAGATATCAACAATGAACAATTGGTTGACATGATACTATTGGATTCTGTGTTCATCATGGAGTTGTTTCTAAGAGAAAACAAAAAATGGGAGCACAAAGATGATTATATAATAACTCAACTTTGTGTAAGTAAAAGTATCCAGCGTGATTTATTGCTTCTTGAGAATCAGCTTCCGATTTATGTGTTGGAAAAACTATATGACACAATTGTTCCAAGCAATGTCAAAAAGCACAATTGGTTTCTCAAGCTTGCACATGAGTATTTTGCATCTTGTTACCCGCATCACAGGGAATCTTCAGAAAGAAAGTTTGAGGCAAAGAAATGGGAGAAATCACGTCATTTCACTGATTTGATTAGATATTCGTATCTCCCTATGAAACTAAAAAATCAGTATAATGATTTTCACAAAGAATGTTTGATGGTAAGGACTGCAACAAAGTTGAATGAAGCTGGTATAAGCTTTGAGAAGGTTCATAACAGATCCTTGCTTGACATAAAGTTTGAGAAAAAACCTTTGTTGAGTTGGTTTCTTTGTTTAGGTTGTTTACCGTGTTGCAAGTGCTTCAAAGCTAGATTTCTATTCCCACAGTTAAAAGTAGATCACGCGACCGAATGTGTTTTGAGGAACCTAATCGCTTTTGAGCAGTGTCACTATCCTGATGAGCCTTACATTTGCAATTATGTTTCTTTGATTGATTCTCTTATTCACACCGAAGAAGACGCAGAGTTGCTAGTTGAGAAAGAAGCAATTGTGCACGAATTGGGAAGCAACGAGGAATTAACGAGCCTTGTTAACAATCTGTGCAAACATGTTGTGACAAATTCGTCGTGTTATTACCAACTTATGGAAGATCTTAATGAACACTACAACAGTGAATGGAAAAGGGCTATGGGTACATTAAAGTGGGTTTATTTTCGTGATCCTTGGAGAAGCAGTTCTACCATAATTGGGATAGCTGTTCTAATATTCACTATCTTTAACTTTCGTCGCGTTGTTGGTCTAATGTTTTAG

Protein sequence

>MS.gene022562.t1

MGSSIEDHIQQIINIPENIEPSHWPQCCIYKVPATLLKVKEEAYTPLLISIGPIHHNNKRLKELQEYKHRYFHYFWNRLDKKSDFMSYKVFLQQEEQNIRRCYQKKFADINNEQLVDMILLDSVFIMELFLRENKKWEHKDDYIITQLCVSKSIQRDLLLLENQLPIYVLEKLYDTIVPSNVKKHNWFLKLAHEYFASCYPHHRESSERKFEAKKWEKSRHFTDLIRYSYLPMKLKNQYNDFHKECLMVRTATKLNEAGISFEKVHNRSLLDIKFEKKPLLSWFLCLGCLPCCKCFKARFLFPQLKVDHATECVLRNLIAFEQCHYPDEPYICNYVSLIDSLIHTEEDAELLVEKEAIVHELGSNEELTSLVNNLCKHVVTNSSCYYQLMEDLNEHYNSEWKRAMGTLKWVYFRDPWRSSSTIIGIAVLIFTIFNFRRVVGLMF