AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037813

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037813.t1 MTR_8g074120 97.963 491 10 0 79 569 20 510 0.0 1003
MS.gene037813.t1 MTR_8g074040 81.786 571 102 1 1 569 31 601 0.0 984
MS.gene037813.t1 MTR_8g074040 80.331 483 93 1 89 569 1 483 0.0 820
MS.gene037813.t1 MTR_5g008800 62.681 552 204 1 13 564 1 550 0.0 749
MS.gene037813.t1 MTR_5g008820 61.029 544 208 2 13 556 1 540 0.0 736
MS.gene037813.t1 MTR_2g044180 52.381 546 249 4 21 555 15 560 0.0 593
MS.gene037813.t1 MTR_4g121600 50.000 564 263 5 8 556 2 561 0.0 587
MS.gene037813.t1 MTR_5g076240 49.458 554 266 7 13 556 1 550 0.0 567
MS.gene037813.t1 MTR_3g064420 48.468 555 270 4 13 553 1 553 0.0 566
MS.gene037813.t1 MTR_5g076220 50.181 554 261 7 13 555 1 550 0.0 533
MS.gene037813.t1 MTR_5g076200 49.911 561 260 8 13 559 1 554 0.0 530
MS.gene037813.t1 MTR_0161s0050 50.446 561 259 8 13 559 1 556 0.0 525
MS.gene037813.t1 MTR_5g023730 48.263 547 272 8 14 554 3 544 1.00e-175 508
MS.gene037813.t1 MTR_2g044180 51.386 469 217 4 98 555 1 469 2.23e-173 499
MS.gene037813.t1 MTR_0803s0020 30.797 552 351 11 17 557 226 757 3.25e-64 228
MS.gene037813.t1 MTR_8g039720 28.240 517 313 16 52 543 65 548 1.02e-45 169
MS.gene037813.t1 MTR_1g026310 26.824 507 333 13 51 543 67 549 7.67e-45 167
MS.gene037813.t1 MTR_2g095510 26.763 553 337 16 26 552 27 537 9.13e-42 158
MS.gene037813.t1 MTR_1569s0010 28.628 503 332 11 64 547 34 528 1.73e-40 154
MS.gene037813.t1 MTR_2g105570 27.573 515 338 15 52 552 54 547 2.11e-40 154
MS.gene037813.t1 MTR_3g035130 27.083 528 338 13 35 548 15 509 3.24e-39 150
MS.gene037813.t1 MTR_4g057730 25.269 558 350 14 22 552 23 540 5.88e-38 147
MS.gene037813.t1 MTR_3g460770 24.490 539 352 18 31 549 33 536 2.46e-37 145
MS.gene037813.t1 MTR_5g007640 27.528 534 333 18 41 552 84 585 1.05e-36 144
MS.gene037813.t1 MTR_4g077900 25.787 508 316 14 64 547 1 471 5.80e-36 140
MS.gene037813.t1 MTR_3g088880 25.046 543 353 14 29 555 53 557 2.71e-35 140
MS.gene037813.t1 MTR_3g088870 25.092 546 352 14 29 555 52 559 4.90e-35 139
MS.gene037813.t1 MTR_5g027500 27.734 512 311 17 64 547 81 561 5.39e-35 139
MS.gene037813.t1 MTR_4g005750 25.926 513 343 13 52 552 53 540 1.57e-34 137
MS.gene037813.t1 MTR_1g073180 26.984 504 321 12 49 543 58 523 2.18e-34 137
MS.gene037813.t1 MTR_1g090737 25.636 511 335 14 53 549 84 563 1.08e-32 132
MS.gene037813.t1 MTR_1g063110 27.559 508 302 18 64 543 68 537 8.90e-32 129
MS.gene037813.t1 MTR_1g073180 26.369 493 317 11 49 533 80 534 4.78e-31 127
MS.gene037813.t1 MTR_1g063120 26.052 499 309 15 72 543 76 541 9.53e-31 126
MS.gene037813.t1 MTR_3g035130 26.305 479 307 11 35 500 15 460 1.72e-29 122
MS.gene037813.t1 MTR_5g027480 26.523 509 309 18 64 547 80 548 6.81e-29 121
MS.gene037813.t1 MTR_2g030240 23.570 577 340 18 32 549 8 542 3.36e-28 119
MS.gene037813.t1 MTR_4g057730 24.124 485 308 13 22 483 23 470 1.86e-27 115
MS.gene037813.t1 MTR_4g128337 24.950 501 319 15 63 541 70 535 3.67e-27 115
MS.gene037813.t1 MTR_1586s0010 26.111 360 233 10 63 408 81 421 1.08e-26 113
MS.gene037813.t1 MTR_2g095510 25.714 455 271 15 26 455 27 439 1.45e-25 110
MS.gene037813.t1 MTR_7g109830 25.309 486 291 18 53 482 78 547 1.26e-24 108
MS.gene037813.t1 MTR_1054s0020 25.720 521 317 18 53 553 301 771 1.72e-24 108
MS.gene037813.t1 MTR_4g011790 25.823 395 245 12 185 546 212 591 1.25e-23 105
MS.gene037813.t1 MTR_1g034360 24.514 514 308 18 22 477 52 543 1.32e-23 105
MS.gene037813.t1 MTR_4g011790 25.693 397 247 12 185 548 212 593 1.72e-23 105
MS.gene037813.t1 MTR_2g030240 24.163 418 253 12 184 550 45 449 2.95e-23 103
MS.gene037813.t1 MTR_4g057730 23.077 468 300 13 22 466 23 453 3.48e-22 100
MS.gene037813.t1 MTR_4g011790 25.369 406 246 13 185 548 212 602 5.59e-22 100
MS.gene037813.t1 MTR_1g086600 22.892 581 351 18 54 566 101 652 1.88e-21 99.0
MS.gene037813.t1 MTR_4g068670 22.649 521 310 16 27 477 44 541 1.79e-20 95.9
MS.gene037813.t1 MTR_4g013210 25.128 390 244 12 192 548 66 440 1.15e-19 92.4
MS.gene037813.t1 MTR_8g099420 24.015 533 299 21 19 470 31 538 1.40e-19 93.2
MS.gene037813.t1 MTR_7g084850 22.980 557 324 19 56 550 207 720 1.78e-19 92.8
MS.gene037813.t1 MTR_4g013210 23.733 375 227 14 192 548 66 399 7.30e-18 86.3
MS.gene037813.t1 MTR_5g009360 23.944 497 281 19 51 477 75 544 8.87e-18 87.4
MS.gene037813.t1 MTR_4g013210 23.733 375 227 14 192 548 218 551 1.04e-17 87.0
MS.gene037813.t1 MTR_5g009350 24.151 530 290 25 51 501 75 571 1.07e-17 87.0
MS.gene037813.t1 MTR_5g009350 24.361 509 278 23 51 484 75 551 1.08e-17 87.0
MS.gene037813.t1 MTR_5g009350 24.303 502 273 23 51 477 75 544 2.42e-17 85.9
MS.gene037813.t1 MTR_5g009350 24.104 502 274 23 51 477 75 544 2.68e-17 85.9
MS.gene037813.t1 MTR_5g009360 26.944 360 174 14 182 477 84 418 5.74e-17 84.3
MS.gene037813.t1 MTR_1g026430 25.080 311 209 8 51 349 67 365 2.47e-16 81.6
MS.gene037813.t1 MTR_1g026470 24.759 311 210 7 51 349 67 365 1.22e-15 79.7
MS.gene037813.t1 MTR_8g083170 29.508 183 103 7 315 485 457 625 4.56e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037813.t1 AT2G17650 67.788 565 170 3 4 556 39 603 0.0 797
MS.gene037813.t1 AT1G20560 61.974 547 206 1 13 557 3 549 0.0 728
MS.gene037813.t1 AT1G20560 61.783 471 178 1 89 557 1 471 0.0 628
MS.gene037813.t1 AT5G16340 52.182 550 250 6 13 554 1 545 0.0 575
MS.gene037813.t1 AT5G16370 52.632 551 248 6 13 555 1 546 0.0 573
MS.gene037813.t1 AT3G16910 48.837 559 274 4 10 556 6 564 0.0 569
MS.gene037813.t1 AT1G66120 48.723 548 274 4 13 553 1 548 0.0 557
MS.gene037813.t1 AT1G76290 50.274 547 262 4 13 556 1 540 0.0 548
MS.gene037813.t1 AT1G75960 49.727 549 263 6 13 553 1 544 0.0 535
MS.gene037813.t1 AT1G65880 46.416 558 280 5 13 553 1 556 0.0 529
MS.gene037813.t1 AT1G65890 46.786 560 274 6 13 553 1 555 0.0 528
MS.gene037813.t1 AT1G21530 47.645 552 274 7 13 553 1 548 0.0 526
MS.gene037813.t1 AT1G21540 48.108 555 268 9 13 553 1 549 0.0 524
MS.gene037813.t1 AT1G21530 47.464 552 273 7 13 553 1 546 0.0 522
MS.gene037813.t1 AT1G77240 47.273 550 275 9 13 553 1 544 1.36e-178 515
MS.gene037813.t1 AT1G68270 43.525 556 268 8 13 553 1 525 4.16e-157 460
MS.gene037813.t1 AT1G20500 28.041 485 304 14 70 540 83 536 9.51e-42 158
MS.gene037813.t1 AT1G20510 27.076 554 344 19 19 543 11 533 2.61e-39 151
MS.gene037813.t1 AT5G63380 27.416 507 314 14 53 543 79 547 1.00e-38 150
MS.gene037813.t1 AT3G21240 26.880 532 350 13 30 549 45 549 1.81e-38 149
MS.gene037813.t1 AT3G48990 27.410 529 344 13 31 548 9 508 4.94e-38 147
MS.gene037813.t1 AT4G05160 26.679 521 325 15 54 552 57 542 5.46e-38 147
MS.gene037813.t1 AT1G20480 26.572 493 326 13 63 543 86 554 6.05e-37 145
MS.gene037813.t1 AT1G62940 25.591 508 340 16 53 549 55 535 5.82e-36 142
MS.gene037813.t1 AT3G21240 27.253 466 301 12 30 484 45 483 2.40e-32 130
MS.gene037813.t1 AT3G21240 27.253 466 301 12 30 484 45 483 2.40e-32 130
MS.gene037813.t1 AT4G19010 24.554 505 319 16 71 553 93 557 2.84e-32 131
MS.gene037813.t1 AT3G21230 26.577 523 340 14 50 555 74 569 5.60e-32 130
MS.gene037813.t1 AT1G30520 24.828 580 330 17 32 549 13 548 1.23e-31 129
MS.gene037813.t1 AT1G30520 24.828 580 330 17 32 549 13 548 1.23e-31 129
MS.gene037813.t1 AT5G38120 26.829 492 304 16 68 540 82 536 2.09e-31 128
MS.gene037813.t1 AT1G65060 24.769 541 367 14 22 549 46 559 4.56e-31 127
MS.gene037813.t1 AT1G20510 26.775 493 302 18 19 483 11 472 5.78e-31 126
MS.gene037813.t1 AT1G51680 24.371 517 346 13 52 549 66 556 7.42e-29 121
MS.gene037813.t1 AT5G38120 26.667 465 286 15 68 514 82 509 2.36e-28 119
MS.gene037813.t1 AT2G47240 25.597 586 329 23 53 566 19 569 2.38e-28 120
MS.gene037813.t1 AT2G47240 25.641 585 328 23 54 566 79 628 3.11e-28 120
MS.gene037813.t1 AT2G47240 25.641 585 328 23 54 566 79 628 3.11e-28 120
MS.gene037813.t1 AT2G47240 25.641 585 328 23 54 566 79 628 3.11e-28 120
MS.gene037813.t1 AT3G21230 26.514 479 314 12 50 513 74 529 2.93e-27 116
MS.gene037813.t1 AT1G51680 24.845 483 316 14 52 513 66 522 4.17e-26 112
MS.gene037813.t1 AT1G20510 26.271 472 289 18 19 462 11 451 7.36e-25 108
MS.gene037813.t1 AT3G21230 26.846 447 292 11 50 484 74 497 2.58e-24 107
MS.gene037813.t1 AT1G64400 24.308 506 292 18 42 480 66 547 1.20e-23 105
MS.gene037813.t1 AT5G38120 26.267 434 265 15 68 483 82 478 2.16e-23 103
MS.gene037813.t1 AT5G38120 26.267 434 265 15 68 483 82 478 2.16e-23 103
MS.gene037813.t1 AT1G51680 25.053 475 305 14 30 484 47 490 2.78e-23 103
MS.gene037813.t1 AT1G30520 24.848 495 278 16 32 479 13 460 4.58e-22 100
MS.gene037813.t1 AT1G30520 24.848 495 278 16 32 479 13 460 4.58e-22 100
MS.gene037813.t1 AT4G11030 25.591 508 264 19 51 480 76 547 2.96e-20 95.1
MS.gene037813.t1 AT1G49430 22.112 502 287 17 52 479 77 548 6.71e-20 94.4
MS.gene037813.t1 AT4G23850 25.301 498 273 22 51 477 76 545 8.31e-20 94.0
MS.gene037813.t1 AT1G65060 24.664 446 297 13 22 455 46 464 2.25e-19 91.7
MS.gene037813.t1 AT1G20490 26.344 372 238 13 70 431 83 428 5.81e-18 87.0
MS.gene037813.t1 AT1G20490 26.344 372 238 13 70 431 83 428 5.81e-18 87.0
MS.gene037813.t1 AT5G36880 22.604 553 349 20 46 550 144 665 5.32e-17 85.1
MS.gene037813.t1 AT5G36880 22.604 553 349 20 46 550 144 665 5.32e-17 85.1
MS.gene037813.t1 AT5G36880 22.604 553 349 20 46 550 144 665 5.32e-17 85.1
MS.gene037813.t1 AT5G36880 22.604 553 349 20 46 550 144 665 5.32e-17 85.1
MS.gene037813.t1 AT5G36880 22.604 553 349 20 46 550 194 715 6.38e-17 84.7

Find 131 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCTTTCCTGTTTATCTT+TGG 0.196891 8.2:-30569284 MS.gene037813:CDS
GACAGAACATCACTTGTTTA+TGG 0.202985 8.2:-30570892 MS.gene037813:CDS
AAGGCCTTGGGAAGCATTTC+TGG 0.208212 8.2:-30568334 MS.gene037813:CDS
TTCACGCCTTGATGCAAATA+TGG 0.236806 8.2:-30569664 MS.gene037813:CDS
TTTGTCACCTATCAACTTCT+TGG 0.240894 8.2:-30570938 MS.gene037813:CDS
ATGGTTCTTTGAAGTATAAT+TGG 0.253303 8.2:-30570873 MS.gene037813:CDS
CTTTCGATGATGGAAGAAAA+TGG 0.256782 8.2:-30569009 MS.gene037813:CDS
GCTTCTTCTCTCTCCCAGTT+AGG 0.257691 8.2:-30570820 MS.gene037813:CDS
GACTTTCCTTAGACAAATAT+TGG 0.272166 8.2:+30569197 None:intergenic
ATATTGTCAAATATGTTCTT+TGG 0.291239 8.2:+30569171 None:intergenic
TTGGAACCAATATTTGTCTA+AGG 0.292344 8.2:-30569203 MS.gene037813:CDS
TCAATGATCATGACTAAAAT+AGG 0.293022 8.2:+30569531 None:intergenic
TCAAATATGCACAAGGAGTT+TGG 0.293728 8.2:+30568512 None:intergenic
GTTCCATTGTTTCATGCTAA+TGG 0.313041 8.2:-30569258 MS.gene037813:CDS
AGATCATTTGCCGCATTTCA+TGG 0.324286 8.2:-30568437 MS.gene037813:CDS
TGGTTCTTTGAAGTATAATT+GGG 0.328576 8.2:-30570872 MS.gene037813:CDS
TTGCTAGACCTGATGATCTA+TGG 0.342123 8.2:-30568537 MS.gene037813:CDS
GAGGTGTAATTTATACTTAT+AGG 0.342668 8.2:+30569396 None:intergenic
TAATGGTTGGTGCTTAGCTT+GGG 0.361141 8.2:-30569241 MS.gene037813:CDS
CTTCTTCTCTCTCCCAGTTA+GGG 0.367339 8.2:-30570819 MS.gene037813:CDS
CTCCACGAGAAATCCCTAAC+TGG 0.368028 8.2:+30570806 None:intergenic
TTTAATATTACTCACATGTA+TGG 0.369041 8.2:-30568985 MS.gene037813:CDS
ATGCTTCTTCTGTTGCTTTG+AGG 0.374387 8.2:+30568731 None:intergenic
TCATACATTGCAGGGACATT+AGG 0.376737 8.2:+30569735 None:intergenic
AGGGATTTCTCGTGGAGATA+TGG 0.387671 8.2:-30570800 MS.gene037813:intron
GGAACATTCCAAAGATAAAC+AGG 0.397537 8.2:+30569276 None:intergenic
GTTGGTGAGGTAATGTTTAG+AGG 0.397931 8.2:-30568784 MS.gene037813:CDS
TTCAACAATGGATAGTGTTC+CGG 0.403704 8.2:-30568821 MS.gene037813:CDS
TGGGGAGTGGCAGCACAGTT+TGG 0.407012 8.2:-30569222 MS.gene037813:CDS
TTGCTTGTGAATGCTCCAAT+AGG 0.413914 8.2:+30569634 None:intergenic
GAGGGAGAAAGCCAAGGCCT+TGG 0.414480 8.2:-30568347 MS.gene037813:CDS
TAGCCATCCAGCGGTCTTAG+AGG 0.414549 8.2:-30568569 MS.gene037813:CDS
AGGACGCTGTTCCAGGATCA+TGG 0.416440 8.2:-30571005 MS.gene037813:CDS
GGGAAGCATTTCTGGACCCC+TGG 0.421191 8.2:-30568326 MS.gene037813:CDS
TCCATTGATTCCCATGATCC+TGG 0.425895 8.2:+30570994 None:intergenic
TCCAAAGATAAACAGGAAAG+AGG 0.427884 8.2:+30569283 None:intergenic
TGCTAGACCTGATGATCTAT+GGG 0.429616 8.2:-30568536 MS.gene037813:CDS
AAATATGGTTTCAGTCCTAT+TGG 0.431796 8.2:-30569649 MS.gene037813:CDS
AGAAATGCTTCCCAAGGCCT+TGG 0.433824 8.2:+30568336 None:intergenic
CTAATGGTTGGTGCTTAGCT+TGG 0.438996 8.2:-30569242 MS.gene037813:CDS
TGACTGAAGACAACTCCTTT+AGG 0.442881 8.2:+30569357 None:intergenic
ACACCTCTGGCACTACATCT+AGG 0.454516 8.2:-30569380 MS.gene037813:CDS
AGGGAGAAAGCCAAGGCCTT+GGG 0.454712 8.2:-30568346 MS.gene037813:CDS
GCTCTCTCCAAGAAGTTGAT+AGG 0.457474 8.2:+30570931 None:intergenic
TTGAAGGACATAATAGTATC+CGG 0.458140 8.2:-30568634 MS.gene037813:CDS
TCCATCATCGAAAGTATTTG+TGG 0.462257 8.2:+30569018 None:intergenic
GTTTGGCCCCATAGATCATC+AGG 0.467905 8.2:+30568529 None:intergenic
CATATTTGAAATTGAAAGAT+GGG 0.469899 8.2:-30568498 MS.gene037813:CDS
ATGGATAGTGTTCCGGCTGA+TGG 0.471080 8.2:-30568814 MS.gene037813:CDS
CACAAGGTATCAGTGATAAC+CGG 0.474826 8.2:-30569057 MS.gene037813:CDS
AAATGCAGCTCATACATTGC+AGG 0.475944 8.2:+30569726 None:intergenic
GATATGCCGAAAACTTCAAC+AGG 0.480240 8.2:-30568391 MS.gene037813:CDS
CGAAGTTGCTCGAGGAGCAT+TGG 0.487528 8.2:-30569583 MS.gene037813:CDS
CTCACAGAAACATGTGGTGC+AGG 0.497007 8.2:-30568961 MS.gene037813:CDS
ATCGAAAGTATTTGTGGCGG+TGG 0.497958 8.2:+30569024 None:intergenic
TCCAGGATCATGGGAATCAA+TGG 0.503093 8.2:-30570995 MS.gene037813:CDS
CTTCTTGGAGAGAGCAGCAA+AGG 0.504058 8.2:-30570923 MS.gene037813:CDS
GAAAGTATTTGTGGCGGTGG+AGG 0.505596 8.2:+30569027 None:intergenic
GCATATTTGAAATTGAAAGA+TGG 0.506247 8.2:-30568499 MS.gene037813:CDS
AAGAAGCATTCAAAGATGGT+TGG 0.515102 8.2:-30568717 MS.gene037813:CDS
CAGAGAATGGCTCCAGCCAT+TGG 0.516988 8.2:+30569696 None:intergenic
CATTTCGCAGTACCAATGGC+TGG 0.521714 8.2:-30569708 MS.gene037813:CDS
TCCAGCGGTCTTAGAGGCTG+CGG 0.522604 8.2:-30568563 MS.gene037813:CDS
CAGCTCAATCGAGGTAGAAA+CGG 0.524106 8.2:-30568599 MS.gene037813:CDS
ATTCGCAATCATATTCAAAA+CGG 0.524788 8.2:+30569110 None:intergenic
GGCACTACATCTAGGCCTAA+AGG 0.526937 8.2:-30569372 MS.gene037813:CDS
ATAAGTATAAATTACACCTC+TGG 0.527089 8.2:-30569393 MS.gene037813:CDS
CATTGTTTCATGCTAATGGT+TGG 0.530364 8.2:-30569254 MS.gene037813:CDS
ATACTATTATGTCCTTCAAC+CGG 0.531022 8.2:+30568638 None:intergenic
GCCACAAATACTTTCGATGA+TGG 0.531993 8.2:-30569019 MS.gene037813:CDS
TATGATTGCGAATTCTACAT+TGG 0.538281 8.2:-30569100 MS.gene037813:CDS
TGAAGGACATAATAGTATCC+GGG 0.542556 8.2:-30568633 MS.gene037813:CDS
TGAATTAAGAGTACAGAGAA+TGG 0.548168 8.2:+30569683 None:intergenic
AAAGATGGTTGGTTTCATAG+TGG 0.548724 8.2:-30568706 MS.gene037813:CDS
TTGTGCTTGGAGACCTGAGT+GGG 0.556554 8.2:-30568932 MS.gene037813:CDS
CTGAAACCATATTTGCATCA+AGG 0.559164 8.2:+30569658 None:intergenic
ACCGCAGCCTCTAAGACCGC+TGG 0.562499 8.2:+30568562 None:intergenic
CGAAATCAAAGACCGGTTGA+AGG 0.562728 8.2:-30568650 MS.gene037813:CDS
TGTCAAAGATCCTTCAACAA+TGG 0.563228 8.2:-30568833 MS.gene037813:CDS
GGACGCTGTTCCAGGATCAT+GGG 0.566750 8.2:-30571004 MS.gene037813:CDS
TTGGTGCTTAGCTTGGGGAG+TGG 0.567534 8.2:-30569235 MS.gene037813:CDS
GGCAAGGTGTACCACATGTA+GGG 0.573061 8.2:-30568870 MS.gene037813:CDS
TTGGTGAGGTAATGTTTAGA+GGG 0.575529 8.2:-30568783 MS.gene037813:CDS
AGGCAAGGTGTACCACATGT+AGG 0.576963 8.2:-30568871 MS.gene037813:CDS
TGGCGGTGGAGGAGAACCTC+CGG 0.578561 8.2:+30569038 None:intergenic
TTCAATTTCAAATATGCACA+AGG 0.580423 8.2:+30568505 None:intergenic
ACAGAAGAAGCATTCAAAGA+TGG 0.580608 8.2:-30568721 MS.gene037813:CDS
TGTACCACATGTAGGGTTGG+AGG 0.582743 8.2:-30568863 MS.gene037813:CDS
TAAGCATAAAGTAACATACA+TGG 0.584854 8.2:-30569145 MS.gene037813:CDS
CCAAAGATAAACAGGAAAGA+GGG 0.591892 8.2:+30569284 None:intergenic
GGATCATGGGAATCAATGGA+AGG 0.598769 8.2:-30570991 MS.gene037813:CDS
GCTGCATTTCGCAGTACCAA+TGG 0.599815 8.2:-30569712 MS.gene037813:CDS
TCCCAGTTAGGGATTTCTCG+TGG 0.600719 8.2:-30570808 MS.gene037813:CDS
GGTTCTTTGAAGTATAATTG+GGG 0.604503 8.2:-30570871 MS.gene037813:CDS
AATGCAGCTCATACATTGCA+GGG 0.604689 8.2:+30569727 None:intergenic
GGAGGTAACATGTCCCACTC+AGG 0.607903 8.2:+30568919 None:intergenic
TCACAGAAACATGTGGTGCA+GGG 0.609788 8.2:-30568960 MS.gene037813:CDS
CAGCCTCTAAGACCGCTGGA+TGG 0.610850 8.2:+30568566 None:intergenic
ACAAAGGAACAGAATTTGCA+TGG 0.610896 8.2:+30570956 None:intergenic
AAGCATAAAGTAACATACAT+GGG 0.612839 8.2:-30569144 MS.gene037813:CDS
AGGGACATTAGGTGCTAGTG+TGG 0.615984 8.2:+30569746 None:intergenic
AGGTGTACCACATGTAGGGT+TGG 0.618177 8.2:-30568866 MS.gene037813:CDS
CTTGCTGTGAAACATTCTGA+TGG 0.618855 8.2:-30568679 MS.gene037813:CDS
TCCACGAGAAATCCCTAACT+GGG 0.623064 8.2:+30570807 None:intergenic
GAAGGACATAATAGTATCCG+GGG 0.624035 8.2:-30568632 MS.gene037813:CDS
AATGGTTGGTGCTTAGCTTG+GGG 0.624167 8.2:-30569240 MS.gene037813:CDS
AGAGGGAATACAATGATGAG+TGG 0.624606 8.2:-30568766 MS.gene037813:CDS
GGGTCCAGAAATGCTTCCCA+AGG 0.629284 8.2:+30568330 None:intergenic
GGAACACTATCCATTGTTGA+AGG 0.629347 8.2:+30568823 None:intergenic
TTTGTGCTTGGAGACCTGAG+TGG 0.630741 8.2:-30568933 MS.gene037813:CDS
CAACCATTAGCATGAAACAA+TGG 0.631816 8.2:+30569255 None:intergenic
GTTACATCGAAATCAAAGAC+CGG 0.638764 8.2:-30568657 MS.gene037813:CDS
TAGAAAACTGCTCAATCACA+AGG 0.640133 8.2:-30569073 MS.gene037813:CDS
AGGCCTAGATGTAGTGCCAG+AGG 0.640784 8.2:+30569377 None:intergenic
AAGAAGTTGATAGGTGACAA+AGG 0.641447 8.2:+30570940 None:intergenic
GCAATCATATTCAAAACGGT+AGG 0.641843 8.2:+30569114 None:intergenic
GATCATGGGAATCAATGGAA+GGG 0.644377 8.2:-30570990 MS.gene037813:CDS
TCAAAGATAAAAGCTAGGCA+AGG 0.649836 8.2:-30568886 MS.gene037813:CDS
CAACTACTCGAAGTTGCTCG+AGG 0.650856 8.2:-30569591 MS.gene037813:CDS
TTATCTAAACTCTCTCGCAA+CGG 0.654030 8.2:-30569325 MS.gene037813:CDS
ATCATCGAAAGTATTTGTGG+CGG 0.662073 8.2:+30569021 None:intergenic
AGAAAACATCAGCTCAATCG+AGG 0.662335 8.2:-30568608 MS.gene037813:CDS
CTGCACCACATGTTTCTGTG+AGG 0.679321 8.2:+30568962 None:intergenic
CATAAAGTAACATACATGGG+AGG 0.691585 8.2:-30569141 MS.gene037813:CDS
AGTTCCTCCAACCCTACATG+TGG 0.698807 8.2:+30568859 None:intergenic
AAGGTATCAGTGATAACCGG+AGG 0.715611 8.2:-30569054 MS.gene037813:CDS
GCTAGACCTGATGATCTATG+GGG 0.730274 8.2:-30568535 MS.gene037813:CDS
ATAGGATCTAATTCACTGCG+TGG 0.732378 8.2:+30569414 None:intergenic
GGAGTTGTCTTCAGTCACAG+AGG 0.737629 8.2:-30569351 MS.gene037813:CDS
AAGGACATAATAGTATCCGG+GGG 0.748378 8.2:-30568631 MS.gene037813:CDS
TATGGCCTCACAGAAACATG+TGG 0.762816 8.2:-30568967 MS.gene037813:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATAAAAATTGAGAT+GGG + chr8.2:30568740-30568759 None:intergenic 10.0%
!! CACAATAAAAAAAATTAATA+TGG + chr8.2:30569301-30569320 None:intergenic 10.0%
!! TAAATAAATAAAAATTGAGA+TGG + chr8.2:30568741-30568760 None:intergenic 10.0%
!!! TTATTTTTTATAAAAGAAAG+TGG - chr8.2:30568758-30568777 MS.gene037813:CDS 10.0%
!! AAAATGTAACATAAATAAGA+AGG + chr8.2:30569223-30569242 None:intergenic 15.0%
!! TAATGTGAAATTTGTATTTA+TGG + chr8.2:30568313-30568332 None:intergenic 15.0%
!!! AAAACAAATTTTGTTAAAAC+TGG - chr8.2:30568812-30568831 MS.gene037813:CDS 15.0%
!!! ACTTTATCTTTAATTTATGT+GGG - chr8.2:30569383-30569402 MS.gene037813:CDS 15.0%
!!! ATTTAATCTTTTATGCATAT+TGG - chr8.2:30568340-30568359 MS.gene037813:CDS 15.0%
!!! TACTTTATCTTTAATTTATG+TGG - chr8.2:30569382-30569401 MS.gene037813:CDS 15.0%
!!! TTTATCTTTGATTTTTCTTT+AGG + chr8.2:30571017-30571036 None:intergenic 15.0%
!! AAAAATCAAAGATAAAAGCT+AGG - chr8.2:30571021-30571040 MS.gene037813:intron 20.0%
!! AATGAATAGAGAATAAGTTT+AGG + chr8.2:30568867-30568886 None:intergenic 20.0%
!! AATGATAACTTGATCAATTA+TGG + chr8.2:30569909-30569928 None:intergenic 20.0%
!! ATAGTACAAAAGAAAAATAG+TGG + chr8.2:30570023-30570042 None:intergenic 20.0%
!! ATATTGTCAAATATGTTCTT+TGG + chr8.2:30570744-30570763 None:intergenic 20.0%
!! ATGAATAGAGAATAAGTTTA+GGG + chr8.2:30568866-30568885 None:intergenic 20.0%
!! ATTGATCAAGTTATCATTTA+AGG - chr8.2:30569911-30569930 MS.gene037813:intron 20.0%
!! GTTAAAATCATATCTATACA+TGG - chr8.2:30569449-30569468 MS.gene037813:CDS 20.0%
!! TTTAATATTACTCACATGTA+TGG - chr8.2:30570927-30570946 MS.gene037813:CDS 20.0%
!!! AAAAATTTCTTTTACAATCC+AGG + chr8.2:30571607-30571626 None:intergenic 20.0%
!!! AAAATTTCTTTTACAATCCA+GGG + chr8.2:30571606-30571625 None:intergenic 20.0%
!!! AACAATTATGCACATATTTT+TGG + chr8.2:30569726-30569745 None:intergenic 20.0%
!!! AATAATGAAAAGATTTTCAC+TGG - chr8.2:30569686-30569705 MS.gene037813:CDS 20.0%
!!! ATAATGATTTTGATATTGTG+AGG - chr8.2:30570475-30570494 MS.gene037813:intron 20.0%
!!! ATCTTTTTTTTTGTTTTTGG+TGG - chr8.2:30568511-30568530 MS.gene037813:CDS 20.0%
!!! CACATCTTTTTTTTTGTTTT+TGG - chr8.2:30568508-30568527 MS.gene037813:CDS 20.0%
!!! CATATTTGAAATTGAAAGAT+GGG - chr8.2:30571414-30571433 MS.gene037813:intron 20.0%
!!! GTAAAAATTATTTTGACAAG+AGG - chr8.2:30568634-30568653 MS.gene037813:CDS 20.0%
!!! TAAAACTTGTTACTTTTTTC+AGG - chr8.2:30568887-30568906 MS.gene037813:CDS 20.0%
! AAGCATAAAGTAACATACAT+GGG - chr8.2:30570768-30570787 MS.gene037813:intron 25.0%
! AGAGAGATAAAATGATAGTT+AGG - chr8.2:30569642-30569661 MS.gene037813:CDS 25.0%
! ATAAGAATCATTCCAACTTT+AGG + chr8.2:30569486-30569505 None:intergenic 25.0%
! ATAAGTATAAATTACACCTC+TGG - chr8.2:30570519-30570538 MS.gene037813:intron 25.0%
! ATGAGTCTCTATTTATACAT+AGG + chr8.2:30569882-30569901 None:intergenic 25.0%
! ATTCGCAATCATATTCAAAA+CGG + chr8.2:30570805-30570824 None:intergenic 25.0%
! GAGAGATAAAATGATAGTTA+GGG - chr8.2:30569643-30569662 MS.gene037813:CDS 25.0%
! GAGGTGTAATTTATACTTAT+AGG + chr8.2:30570519-30570538 None:intergenic 25.0%
! TAAACCAATTAACATTGTTG+AGG - chr8.2:30568380-30568399 MS.gene037813:CDS 25.0%
! TAAGCATAAAGTAACATACA+TGG - chr8.2:30570767-30570786 MS.gene037813:intron 25.0%
! TCAATGATCATGACTAAAAT+AGG + chr8.2:30570384-30570403 None:intergenic 25.0%
! TCCAACACACATAAAATTTA+AGG - chr8.2:30568405-30568424 MS.gene037813:CDS 25.0%
! TGAAAATGAAGAAGATAACA+AGG + chr8.2:30569851-30569870 None:intergenic 25.0%
! TTAAGGTCATTTGTCTAATT+AGG - chr8.2:30569928-30569947 MS.gene037813:intron 25.0%
! TTCAATTTCAAATATGCACA+AGG + chr8.2:30571410-30571429 None:intergenic 25.0%
!! AAATTTCTTTTACAATCCAG+GGG + chr8.2:30571605-30571624 None:intergenic 25.0%
!! ACCTTAAATTTTATGTGTGT+TGG + chr8.2:30568409-30568428 None:intergenic 25.0%
!! ATTTCATGTTGATTGTAAAC+AGG - chr8.2:30570140-30570159 MS.gene037813:intron 25.0%
!! CAACAATGTTAATTGGTTTA+AGG + chr8.2:30568380-30568399 None:intergenic 25.0%
!! CATAAACACAAATTTTACAG+CGG + chr8.2:30568438-30568457 None:intergenic 25.0%
!! GCATATTTGAAATTGAAAGA+TGG - chr8.2:30571413-30571432 MS.gene037813:intron 25.0%
!! TCTTGAAAAATGACTGTTTT+AGG + chr8.2:30571503-30571522 None:intergenic 25.0%
!!! AGATACAAAAGTTTGTTTTG+AGG - chr8.2:30571546-30571565 MS.gene037813:intron 25.0%
!!! ATCTTTGATTTTTCTTTAGG+AGG + chr8.2:30571014-30571033 None:intergenic 25.0%
!!! ATGGTTCTTTGAAGTATAAT+TGG - chr8.2:30569039-30569058 MS.gene037813:CDS 25.0%
!!! GATACAAAAGTTTGTTTTGA+GGG - chr8.2:30571547-30571566 MS.gene037813:intron 25.0%
!!! TCTTTTTTCACTATTGTGTT+TGG - chr8.2:30569250-30569269 MS.gene037813:CDS 25.0%
!!! TGGTTCTTTGAAGTATAATT+GGG - chr8.2:30569040-30569059 MS.gene037813:CDS 25.0%
!!! TTTTTTTTGTTTTTGGTGGT+CGG - chr8.2:30568515-30568534 MS.gene037813:CDS 25.0%
AAAATCAGTTTGTAAGTGAG+AGG - chr8.2:30569334-30569353 MS.gene037813:CDS 30.0%
AAAATGATAGTTAGGGTCTT+AGG - chr8.2:30569650-30569669 MS.gene037813:CDS 30.0%
AAATATGGTTTCAGTCCTAT+TGG - chr8.2:30570263-30570282 MS.gene037813:intron 30.0%
AAATGATAGTTAGGGTCTTA+GGG - chr8.2:30569651-30569670 MS.gene037813:CDS 30.0%
AAGAAGATAACAAGGATAGT+AGG + chr8.2:30569843-30569862 None:intergenic 30.0%
ATACTATTATGTCCTTCAAC+CGG + chr8.2:30571277-30571296 None:intergenic 30.0%
GACAATGCATAATATATGCA+CGG + chr8.2:30568556-30568575 None:intergenic 30.0%
GACATCTAACTAAACTAACA+TGG - chr8.2:30568612-30568631 MS.gene037813:CDS 30.0%
GACTTTCCTTAGACAAATAT+TGG + chr8.2:30570718-30570737 None:intergenic 30.0%
TAACTAAGCTAAGCTAACTA+GGG - chr8.2:30568581-30568600 MS.gene037813:CDS 30.0%
TATGATTGCGAATTCTACAT+TGG - chr8.2:30570812-30570831 MS.gene037813:CDS 30.0%
TTAGCTTAGCTTAGTTAGTA+GGG + chr8.2:30568578-30568597 None:intergenic 30.0%
TTGAAGGACATAATAGTATC+CGG - chr8.2:30571278-30571297 MS.gene037813:intron 30.0%
TTGGAACCAATATTTGTCTA+AGG - chr8.2:30570709-30570728 MS.gene037813:intron 30.0%
TTTATCTCTCTTCATGACAA+TGG + chr8.2:30569633-30569652 None:intergenic 30.0%
TTTCCAAGTGTATGACAATT+TGG + chr8.2:30568703-30568722 None:intergenic 30.0%
! ATCTTACCTGTTGAAGTTTT+CGG + chr8.2:30571530-30571549 None:intergenic 30.0%
! GGTGTTAAGTTTTCAGAAAT+TGG - chr8.2:30569973-30569992 MS.gene037813:intron 30.0%
! TAGGGTGATAGTAATGTTTT+AGG - chr8.2:30569189-30569208 MS.gene037813:CDS 30.0%
! TGAATTAAGAGTACAGAGAA+TGG + chr8.2:30570232-30570251 None:intergenic 30.0%
! TTTTTCACTATTGTGTTTGG+TGG - chr8.2:30569253-30569272 MS.gene037813:CDS 30.0%
!! GGTTCTTTGAAGTATAATTG+GGG - chr8.2:30569041-30569060 MS.gene037813:CDS 30.0%
!!! GCACATATTTTTGGAAACAA+TGG + chr8.2:30569717-30569736 None:intergenic 30.0%
!!! TTTTTTTGTTTTTGGTGGTC+GGG - chr8.2:30568516-30568535 MS.gene037813:CDS 30.0%
ACAAAGGAACAGAATTTGCA+TGG + chr8.2:30568959-30568978 None:intergenic 35.0%
AGGTCATTTGTCTAATTAGG+TGG - chr8.2:30569931-30569950 MS.gene037813:intron 35.0%
ATGCCAAATTGTCATACACT+TGG - chr8.2:30568697-30568716 MS.gene037813:CDS 35.0%
CAACCATTAGCATGAAACAA+TGG + chr8.2:30570660-30570679 None:intergenic 35.0%
CATAAAGTAACATACATGGG+AGG - chr8.2:30570771-30570790 MS.gene037813:intron 35.0%
CATAATATATGCACGGTTCA+GGG + chr8.2:30568549-30568568 None:intergenic 35.0%
CATTGTTTCATGCTAATGGT+TGG - chr8.2:30570658-30570677 MS.gene037813:intron 35.0%
CCAAAGATAAACAGGAAAGA+GGG + chr8.2:30570631-30570650 None:intergenic 35.0%
CTAACTAAGCTAAGCTAACT+AGG - chr8.2:30568580-30568599 MS.gene037813:CDS 35.0%
CTATCACTGTTCTCACTTTA+GGG - chr8.2:30569171-30569190 MS.gene037813:CDS 35.0%
CTGAAACCATATTTGCATCA+AGG + chr8.2:30570257-30570276 None:intergenic 35.0%
GACAGAACATCACTTGTTTA+TGG - chr8.2:30569020-30569039 MS.gene037813:CDS 35.0%
GCAATCATATTCAAAACGGT+AGG + chr8.2:30570801-30570820 None:intergenic 35.0%
GGAACATTCCAAAGATAAAC+AGG + chr8.2:30570639-30570658 None:intergenic 35.0%
GTTACATCGAAATCAAAGAC+CGG - chr8.2:30571255-30571274 MS.gene037813:intron 35.0%
GTTCATGCAATTCCTAAAGT+TGG - chr8.2:30569471-30569490 MS.gene037813:CDS 35.0%
GTTCCATTGTTTCATGCTAA+TGG - chr8.2:30570654-30570673 MS.gene037813:intron 35.0%
TAGAAAACTGCTCAATCACA+AGG - chr8.2:30570839-30570858 MS.gene037813:CDS 35.0%
TATGCATATTGGACTTTCGT+CGG - chr8.2:30568351-30568370 MS.gene037813:CDS 35.0%
TCAAAGATAAAAGCTAGGCA+AGG - chr8.2:30571026-30571045 MS.gene037813:intron 35.0%
TCAAATATGCACAAGGAGTT+TGG + chr8.2:30571403-30571422 None:intergenic 35.0%
TCATTTGTCTAATTAGGTGG+TGG - chr8.2:30569934-30569953 MS.gene037813:intron 35.0%
TCCAAAGATAAACAGGAAAG+AGG + chr8.2:30570632-30570651 None:intergenic 35.0%
TCTATCACTGTTCTCACTTT+AGG - chr8.2:30569170-30569189 MS.gene037813:CDS 35.0%
TGAAGGACATAATAGTATCC+GGG - chr8.2:30571279-30571298 MS.gene037813:intron 35.0%
TGTCAAAGATCCTTCAACAA+TGG - chr8.2:30571079-30571098 MS.gene037813:intron 35.0%
TTATCTAAACTCTCTCGCAA+CGG - chr8.2:30570587-30570606 MS.gene037813:intron 35.0%
TTCAACAATGGATAGTGTTC+CGG - chr8.2:30571091-30571110 MS.gene037813:intron 35.0%
TTGGTGAGGTAATGTTTAGA+GGG - chr8.2:30571129-30571148 MS.gene037813:intron 35.0%
TTTGTCACCTATCAACTTCT+TGG - chr8.2:30568974-30568993 MS.gene037813:CDS 35.0%
! GTGATTGAGCAGTTTTCTAT+CGG + chr8.2:30570838-30570857 None:intergenic 35.0%
! GTTAGCTTAGCTTAGTTAGT+AGG + chr8.2:30568579-30568598 None:intergenic 35.0%
!! AAAGATGGTTGGTTTCATAG+TGG - chr8.2:30571206-30571225 MS.gene037813:intron 35.0%
!! AAGAAGCATTCAAAGATGGT+TGG - chr8.2:30571195-30571214 MS.gene037813:intron 35.0%
!! AAGAAGTTGATAGGTGACAA+AGG + chr8.2:30568975-30568994 None:intergenic 35.0%
!! ACAGAAGAAGCATTCAAAGA+TGG - chr8.2:30571191-30571210 MS.gene037813:intron 35.0%
!! ATCATCGAAAGTATTTGTGG+CGG + chr8.2:30570894-30570913 None:intergenic 35.0%
!! CTTTCGATGATGGAAGAAAA+TGG - chr8.2:30570903-30570922 MS.gene037813:CDS 35.0%
!! TCCATCATCGAAAGTATTTG+TGG + chr8.2:30570897-30570916 None:intergenic 35.0%
!!! TTTTTTGTTTTTGGTGGTCG+GGG - chr8.2:30568517-30568536 MS.gene037813:CDS 35.0%
AAATGCAGCTCATACATTGC+AGG + chr8.2:30570189-30570208 None:intergenic 40.0%
AAGGACATAATAGTATCCGG+GGG - chr8.2:30571281-30571300 MS.gene037813:intron 40.0%
AATGCAGCTCATACATTGCA+GGG + chr8.2:30570188-30570207 None:intergenic 40.0%
AGAAAACATCAGCTCAATCG+AGG - chr8.2:30571304-30571323 MS.gene037813:intron 40.0%
AGAGGGAATACAATGATGAG+TGG - chr8.2:30571146-30571165 MS.gene037813:intron 40.0%
AGATCATTTGCCGCATTTCA+TGG - chr8.2:30571475-30571494 MS.gene037813:intron 40.0%
ATAGGATCTAATTCACTGCG+TGG + chr8.2:30570501-30570520 None:intergenic 40.0%
CCCTCTTTCCTGTTTATCTT+TGG - chr8.2:30570628-30570647 MS.gene037813:intron 40.0%
GAAGGACATAATAGTATCCG+GGG - chr8.2:30571280-30571299 MS.gene037813:intron 40.0%
GACGCCTCAACAATGTTAAT+TGG + chr8.2:30568387-30568406 None:intergenic 40.0%
GATATGCCGAAAACTTCAAC+AGG - chr8.2:30571521-30571540 MS.gene037813:intron 40.0%
GATCATGGGAATCAATGGAA+GGG - chr8.2:30568922-30568941 MS.gene037813:CDS 40.0%
GCATAATATATGCACGGTTC+AGG + chr8.2:30568550-30568569 None:intergenic 40.0%
GCCACAAATACTTTCGATGA+TGG - chr8.2:30570893-30570912 MS.gene037813:CDS 40.0%
GGAACACTATCCATTGTTGA+AGG + chr8.2:30571092-30571111 None:intergenic 40.0%
TCATACATTGCAGGGACATT+AGG + chr8.2:30570180-30570199 None:intergenic 40.0%
TGACTGAAGACAACTCCTTT+AGG + chr8.2:30570558-30570577 None:intergenic 40.0%
TGCTAGACCTGATGATCTAT+GGG - chr8.2:30571376-30571395 MS.gene037813:intron 40.0%
TTCACGCCTTGATGCAAATA+TGG - chr8.2:30570248-30570267 MS.gene037813:intron 40.0%
TTGCTAGACCTGATGATCTA+TGG - chr8.2:30571375-30571394 MS.gene037813:intron 40.0%
TTGCTTGTGAATGCTCCAAT+AGG + chr8.2:30570281-30570300 None:intergenic 40.0%
! AGGCTTTCTTTTCGCTCTTT+TGG + chr8.2:30570364-30570383 None:intergenic 40.0%
! CACAAGGTATCAGTGATAAC+CGG - chr8.2:30570855-30570874 MS.gene037813:CDS 40.0%
! CTTGCTGTGAAACATTCTGA+TGG - chr8.2:30571233-30571252 MS.gene037813:intron 40.0%
! GTAACTTGTGCGTTGATGTT+AGG + chr8.2:30568470-30568489 None:intergenic 40.0%
! GTTGGTGAGGTAATGTTTAG+AGG - chr8.2:30571128-30571147 MS.gene037813:intron 40.0%
! TAATGGTTGGTGCTTAGCTT+GGG - chr8.2:30570671-30570690 MS.gene037813:intron 40.0%
!! ATGCTTCTTCTGTTGCTTTG+AGG + chr8.2:30571184-30571203 None:intergenic 40.0%
!! GACAAGAGGTGTCAATTTCA+GGG - chr8.2:30568648-30568667 MS.gene037813:CDS 40.0%
!! GGTGGTGATTGATTTTGCTA+GGG - chr8.2:30569952-30569971 MS.gene037813:intron 40.0%
!! TGACAAGAGGTGTCAATTTC+AGG - chr8.2:30568647-30568666 MS.gene037813:CDS 40.0%
!! TGGTGGTGATTGATTTTGCT+AGG - chr8.2:30569951-30569970 MS.gene037813:intron 40.0%
!! TTCTTTTCGCTCTTTTGGCT+AGG + chr8.2:30570359-30570378 None:intergenic 40.0%
!!! GTTTTAGGAGCCATGAAATG+CGG + chr8.2:30571488-30571507 None:intergenic 40.0%
AGGGATTTCTCGTGGAGATA+TGG - chr8.2:30569112-30569131 MS.gene037813:CDS 45.0%
CAGCTCAATCGAGGTAGAAA+CGG - chr8.2:30571313-30571332 MS.gene037813:intron 45.0%
CGAAATCAAAGACCGGTTGA+AGG - chr8.2:30571262-30571281 MS.gene037813:intron 45.0%
CTTCTTCTCTCTCCCAGTTA+GGG - chr8.2:30569093-30569112 MS.gene037813:CDS 45.0%
GCTAGACCTGATGATCTATG+GGG - chr8.2:30571377-30571396 MS.gene037813:intron 45.0%
GGATCATGGGAATCAATGGA+AGG - chr8.2:30568921-30568940 MS.gene037813:CDS 45.0%
TATGGCCTCACAGAAACATG+TGG - chr8.2:30570945-30570964 MS.gene037813:CDS 45.0%
TCACAGAAACATGTGGTGCA+GGG - chr8.2:30570952-30570971 MS.gene037813:CDS 45.0%
TCCACGAGAAATCCCTAACT+GGG + chr8.2:30569108-30569127 None:intergenic 45.0%
TCCAGGATCATGGGAATCAA+TGG - chr8.2:30568917-30568936 MS.gene037813:CDS 45.0%
TGTTCCGGCTGATGGAAAAA+CGG - chr8.2:30571106-30571125 MS.gene037813:intron 45.0%
! AAGGTATCAGTGATAACCGG+AGG - chr8.2:30570858-30570877 MS.gene037813:CDS 45.0%
! AATGGTTGGTGCTTAGCTTG+GGG - chr8.2:30570672-30570691 MS.gene037813:intron 45.0%
! CTAATGGTTGGTGCTTAGCT+TGG - chr8.2:30570670-30570689 MS.gene037813:intron 45.0%
! GCTCTCTCCAAGAAGTTGAT+AGG + chr8.2:30568984-30569003 None:intergenic 45.0%
! GGTATCAGAGCCTATCGATT+CGG + chr8.2:30569280-30569299 None:intergenic 45.0%
! TGATGGAAAAACGGTTGGTG+AGG - chr8.2:30571115-30571134 MS.gene037813:intron 45.0%
!! ATCGAAAGTATTTGTGGCGG+TGG + chr8.2:30570891-30570910 None:intergenic 45.0%
!! GGTTTTGTATAGCCATCCAG+CGG - chr8.2:30571334-30571353 MS.gene037813:intron 45.0%
!! TCCATTGATTCCCATGATCC+TGG + chr8.2:30568921-30568940 None:intergenic 45.0%
!! TTTTCGCTCTTTTGGCTAGG+AGG + chr8.2:30570356-30570375 None:intergenic 45.0%
!!! TGTTTTGAGGGAGAAAGCCA+AGG - chr8.2:30571559-30571578 MS.gene037813:intron 45.0%
!!! TTTTTTCAGGACGCTGTTCC+AGG - chr8.2:30568900-30568919 MS.gene037813:CDS 45.0%
ACACCTCTGGCACTACATCT+AGG - chr8.2:30570532-30570551 MS.gene037813:intron 50.0%
AGAAATGCTTCCCAAGGCCT+TGG + chr8.2:30571579-30571598 None:intergenic 50.0%
AGGCAAGGTGTACCACATGT+AGG - chr8.2:30571041-30571060 MS.gene037813:intron 50.0%
AGGGACATTAGGTGCTAGTG+TGG + chr8.2:30570169-30570188 None:intergenic 50.0%
AGGTGTACCACATGTAGGGT+TGG - chr8.2:30571046-30571065 MS.gene037813:intron 50.0%
AGTGGCATCTCGAAACTGAG+CGG + chr8.2:30570005-30570024 None:intergenic 50.0%
AGTTCCTCCAACCCTACATG+TGG + chr8.2:30571056-30571075 None:intergenic 50.0%
ATGGATAGTGTTCCGGCTGA+TGG - chr8.2:30571098-30571117 MS.gene037813:intron 50.0%
CAACTACTCGAAGTTGCTCG+AGG - chr8.2:30570321-30570340 MS.gene037813:intron 50.0%
CATTTCGCAGTACCAATGGC+TGG - chr8.2:30570204-30570223 MS.gene037813:intron 50.0%
CTCACAGAAACATGTGGTGC+AGG - chr8.2:30570951-30570970 MS.gene037813:CDS 50.0%
CTCCACGAGAAATCCCTAAC+TGG + chr8.2:30569109-30569128 None:intergenic 50.0%
CTTCTTGGAGAGAGCAGCAA+AGG - chr8.2:30568989-30569008 MS.gene037813:CDS 50.0%
GCACTGTGAACTGTGCTTGT+TGG - chr8.2:30570077-30570096 MS.gene037813:intron 50.0%
GCTGCATTTCGCAGTACCAA+TGG - chr8.2:30570200-30570219 MS.gene037813:intron 50.0%
GCTTCTTCTCTCTCCCAGTT+AGG - chr8.2:30569092-30569111 MS.gene037813:CDS 50.0%
GGAGTTGTCTTCAGTCACAG+AGG - chr8.2:30570561-30570580 MS.gene037813:intron 50.0%
GGCAAGGTGTACCACATGTA+GGG - chr8.2:30571042-30571061 MS.gene037813:intron 50.0%
GGCACTACATCTAGGCCTAA+AGG - chr8.2:30570540-30570559 MS.gene037813:intron 50.0%
GTTTGGCCCCATAGATCATC+AGG + chr8.2:30571386-30571405 None:intergenic 50.0%
TCCCAGTTAGGGATTTCTCG+TGG - chr8.2:30569104-30569123 MS.gene037813:CDS 50.0%
TGTACCACATGTAGGGTTGG+AGG - chr8.2:30571049-30571068 MS.gene037813:intron 50.0%
TTGTGCTTGGAGACCTGAGT+GGG - chr8.2:30570980-30570999 MS.gene037813:CDS 50.0%
TTTGTGCTTGGAGACCTGAG+TGG - chr8.2:30570979-30570998 MS.gene037813:CDS 50.0%
! CTGCACCACATGTTTCTGTG+AGG + chr8.2:30570953-30570972 None:intergenic 50.0%
! GTGCAGGGACTTTTTGTGCT+TGG - chr8.2:30570967-30570986 MS.gene037813:CDS 50.0%
! GTTTGGTGGTCCGAATCGAT+AGG - chr8.2:30569267-30569286 MS.gene037813:CDS 50.0%
! TGAGCTGATGTTTTCTCCCC+CGG + chr8.2:30571300-30571319 None:intergenic 50.0%
!! AAGGCCTTGGGAAGCATTTC+TGG - chr8.2:30571578-30571597 MS.gene037813:intron 50.0%
!! CCAACCGTTTTTCCATCAGC+CGG + chr8.2:30571113-30571132 None:intergenic 50.0%
!! GAAAGTATTTGTGGCGGTGG+AGG + chr8.2:30570888-30570907 None:intergenic 50.0%
!! GTTGGCTTTGATGCTCAGCA+AGG - chr8.2:30570095-30570114 MS.gene037813:intron 50.0%
AGGACGCTGTTCCAGGATCA+TGG - chr8.2:30568907-30568926 MS.gene037813:CDS 55.0%
AGGCCTAGATGTAGTGCCAG+AGG + chr8.2:30570538-30570557 None:intergenic 55.0%
AGGGAGAAAGCCAAGGCCTT+GGG - chr8.2:30571566-30571585 MS.gene037813:intron 55.0%
CAGAGAATGGCTCCAGCCAT+TGG + chr8.2:30570219-30570238 None:intergenic 55.0%
CCGGCTGATGGAAAAACGGT+TGG - chr8.2:30571110-30571129 MS.gene037813:intron 55.0%
GGACGCTGTTCCAGGATCAT+GGG - chr8.2:30568908-30568927 MS.gene037813:CDS 55.0%
GGAGGTAACATGTCCCACTC+AGG + chr8.2:30570996-30571015 None:intergenic 55.0%
GGGTCCAGAAATGCTTCCCA+AGG + chr8.2:30571585-30571604 None:intergenic 55.0%
TAGCCATCCAGCGGTCTTAG+AGG - chr8.2:30571343-30571362 MS.gene037813:intron 55.0%
! CGAAGTTGCTCGAGGAGCAT+TGG - chr8.2:30570329-30570348 MS.gene037813:intron 55.0%
! TTGGTGCTTAGCTTGGGGAG+TGG - chr8.2:30570677-30570696 MS.gene037813:intron 55.0%
GAGGGAGAAAGCCAAGGCCT+TGG - chr8.2:30571565-30571584 MS.gene037813:intron 60.0%
TCCAGCGGTCTTAGAGGCTG+CGG - chr8.2:30571349-30571368 MS.gene037813:intron 60.0%
TGGGGAGTGGCAGCACAGTT+TGG - chr8.2:30570690-30570709 MS.gene037813:intron 60.0%
!! CAGCCTCTAAGACCGCTGGA+TGG + chr8.2:30571349-30571368 None:intergenic 60.0%
!! GGGAAGCATTTCTGGACCCC+TGG - chr8.2:30571586-30571605 MS.gene037813:intron 60.0%
TGGCGGTGGAGGAGAACCTC+CGG + chr8.2:30570877-30570896 None:intergenic 65.0%
!! ACCGCAGCCTCTAAGACCGC+TGG + chr8.2:30571353-30571372 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 30568297 30571637 30568297 ID=MS.gene037813
chr8.2 mRNA 30568297 30571637 30568297 ID=MS.gene037813.t1;Parent=MS.gene037813
chr8.2 exon 30571629 30571637 30571629 ID=MS.gene037813.t1.exon1;Parent=MS.gene037813.t1
chr8.2 CDS 30571629 30571637 30571629 ID=cds.MS.gene037813.t1;Parent=MS.gene037813.t1
chr8.2 exon 30570801 30571025 30570801 ID=MS.gene037813.t1.exon2;Parent=MS.gene037813.t1
chr8.2 CDS 30570801 30571025 30570801 ID=cds.MS.gene037813.t1;Parent=MS.gene037813.t1
chr8.2 exon 30568297 30569772 30568297 ID=MS.gene037813.t1.exon3;Parent=MS.gene037813.t1
chr8.2 CDS 30568297 30569772 30568297 ID=cds.MS.gene037813.t1;Parent=MS.gene037813.t1
Gene Sequence

>MS.gene037813

ATGTCACATGTAACCATAAATACAAATTTCACATTAATTGAAAATTTAATCTTTTATGCATATTGGACTTTCGTCGGAAACCTTAAACCAATTAACATTGTTGAGGCGTCCAACACACATAAAATTTAAGGTTGTTAACCGCTGTAAAATTTGTGTTTATGATGTTGTGACCTAACATCAACGCACAAGTTACAATAACATGACACACTAACACATCTTTTTTTTTGTTTTTGGTGGTCGGGGTTTGAACCCTGAACCGTGCATATATTATGCATTGTCCCTACTAACTAAGCTAAGCTAACTAGGGCACACTAAGACATCTAACTAAACTAACATGGTAAAAATTATTTTGACAAGAGGTGTCAATTTCAGGGAGCAATTCAATATTTGTAAATTTGAAATGCCAAATTGTCATACACTTGGAAATTAAAATTTCAATTCCCATCTCAATTTTTATTTATTTATTTTTTATAAAAGAAAGTGGTTTAAAACAAATTTTCGAACAATGAACAATTAAAACAAATTTTGTTAAAACTGGTTTCGTTTTTCTTTTATTTTTCAGCATGCCCTAAACTTATTCTCTATTCATTTAAAACTTGTTACTTTTTTCAGGACGCTGTTCCAGGATCATGGGAATCAATGGAAGGGCTAGTTCATTGCCATGCAAATTCTGTTCCTTTGTCACCTATCAACTTCTTGGAGAGAGCAGCAAAGGTTTGTAGAGACAGAACATCACTTGTTTATGGTTCTTTGAAGTATAATTGGGGTCAAACTTATCAACGTTGTCTCAAACTTGCTTCTTCTCTCTCCCAGTTAGGGATTTCTCGTGGAGATATGGTCAGTTTTCTCTCTATCTATCTATCTATCTATCTATCTATCACTGTTCTCACTTTAGGGTGATAGTAATGTTTTAGGATTTTTTTCCTTCTTATTTATGTTACATTTTTCTTGTTTCTTTTTTCACTATTGTGTTTGGTGGTCCGAATCGATAGGCTCTGATACCATATTAATTTTTTTTATTGTGTCAAATTATTTACAAAATCAGTTTGTAAGTGAGAGGTACGTTTGTTTATAAACTCTAGTCATACTTTATCTTTAATTTATGTGGGACTTAGTTTTTTCAATACTACAGTTAGTGCTCTTAGAAATTTTGTTAAAATCATATCTATACATGGTTCATGCAATTCCTAAAGTTGGAATGATTCTTATTAGAGATAAATCTTGTTATTAGTTCGTTATGTTAAGTTTACTTTGCGAATTTTCTAATTTCATTTGTTAAAATATGAGTTCATTTTAACTTGCTAATAAGAAGATGTGAGACTATTGAAATGAACCATTGTCATGAAGAGAGATAAAATGATAGTTAGGGTCTTAGGGATACTTTCATGAAATAATGAAAAGATTTTCACTGGCATTTCCATTGTTTCCAAAAATATGTGCATAATTGTTCACTTCAAGAGTCTGTTATCTCTTTGTCAGCAAGTAAAATTAACACAAATTTTAGAAAATGAAAATAGAAAATACTATCACAAAGTAAATGTCACCTACTATCCTTGTTATCTTCTTCATTTTCATTTAGAATCCTATGTATAAATAGAGACTCATGTAGCCATAATTGATCAAGTTATCATTTAAGGTCATTTGTCTAATTAGGTGGTGGTGATTGATTTTGCTAGGGTGTTAAGTTTTCAGAAATTGGCTATCACCGCTCAGTTTCGAGATGCCACTATTTTTCTTTTGTACTATCTTGCATTGATGTTTCTATAAAATGTGTTGCACTGCACTGTGAACTGTGCTTGTTGGCTTTGATGCTCAGCAAGGTGAATGAACAATCACTGATGCTATTTCATGTTGATTGTAAACAGGTTGCCACACTAGCACCTAATGTCCCTGCAATGTATGAGCTGCATTTCGCAGTACCAATGGCTGGAGCCATTCTCTGTACTCTTAATTCACGCCTTGATGCAAATATGGTTTCAGTCCTATTGGAGCATTCACAAGCAAAGATTCTATTCGTAGACTATCAACTACTCGAAGTTGCTCGAGGAGCATTGGACCTCCTAGCCAAAAGAGCGAAAAGAAAGCCTATTTTAGTCATGATCATTGACTCTGATTGCACATCTAATATCGACATTACTTCAATTAGTTATGAATATGAAAAACTTATAGCTATTGAACATAATGATTTTGATATTGTGAGGCCACGCAGTGAATTAGATCCTATAAGTATAAATTACACCTCTGGCACTACATCTAGGCCTAAAGGAGTTGTCTTCAGTCACAGAGGTGCTTATCTAAACTCTCTCGCAACGGTTCTACTCTTACAAATGACCCTCTTTCCTGTTTATCTTTGGAATGTTCCATTGTTTCATGCTAATGGTTGGTGCTTAGCTTGGGGAGTGGCAGCACAGTTTGGAACCAATATTTGTCTAAGGAAAGTCTCTCCAAAGAACATATTTGACAATATAATTAAGCATAAAGTAACATACATGGGAGGAGCTCCTACCGTTTTGAATATGATTGCGAATTCTACATTGGCCGATAGAAAACTGCTCAATCACAAGGTATCAGTGATAACCGGAGGTTCTCCTCCACCGCCACAAATACTTTCGATGATGGAAGAAAATGGTTTTAATATTACTCACATGTATGGCCTCACAGAAACATGTGGTGCAGGGACTTTTTGTGCTTGGAGACCTGAGTGGGACATGTTACCTCCTAAAGAAAAATCAAAGATAAAAGCTAGGCAAGGTGTACCACATGTAGGGTTGGAGGAACTTGATGTCAAAGATCCTTCAACAATGGATAGTGTTCCGGCTGATGGAAAAACGGTTGGTGAGGTAATGTTTAGAGGGAATACAATGATGAGTGGTTATTTTCAAGACCTCAAAGCAACAGAAGAAGCATTCAAAGATGGTTGGTTTCATAGTGGCGATCTTGCTGTGAAACATTCTGATGGTTACATCGAAATCAAAGACCGGTTGAAGGACATAATAGTATCCGGGGGAGAAAACATCAGCTCAATCGAGGTAGAAACGGTTTTGTATAGCCATCCAGCGGTCTTAGAGGCTGCGGTTGTTGCTAGACCTGATGATCTATGGGGCCAAACTCCTTGTGCATATTTGAAATTGAAAGATGGGTTTGATGCAGATGTTCAAGAAATAATAAACTTTTGTAGAGATCATTTGCCGCATTTCATGGCTCCTAAAACAGTCATTTTTCAAGATATGCCGAAAACTTCAACAGGTAAGATACAAAAGTTTGTTTTGAGGGAGAAAGCCAAGGCCTTGGGAAGCATTTCTGGACCCCTGGATTGTAAAAGAAATTTTTTTAAGTGCTGA
Protein sequence

>MS.gene037813.t1

MSHDAVPGSWESMEGLVHCHANSVPLSPINFLERAAKVCRDRTSLVYGSLKYNWGQTYQRCLKLASSLSQLGISRGDMVATLAPNVPAMYELHFAVPMAGAILCTLNSRLDANMVSVLLEHSQAKILFVDYQLLEVARGALDLLAKRAKRKPILVMIIDSDCTSNIDITSISYEYEKLIAIEHNDFDIVRPRSELDPISINYTSGTTSRPKGVVFSHRGAYLNSLATVLLLQMTLFPVYLWNVPLFHANGWCLAWGVAAQFGTNICLRKVSPKNIFDNIIKHKVTYMGGAPTVLNMIANSTLADRKLLNHKVSVITGGSPPPPQILSMMEENGFNITHMYGLTETCGAGTFCAWRPEWDMLPPKEKSKIKARQGVPHVGLEELDVKDPSTMDSVPADGKTVGEVMFRGNTMMSGYFQDLKATEEAFKDGWFHSGDLAVKHSDGYIEIKDRLKDIIVSGGENISSIEVETVLYSHPAVLEAAVVARPDDLWGQTPCAYLKLKDGFDADVQEIINFCRDHLPHFMAPKTVIFQDMPKTSTGKIQKFVLREKAKALGSISGPLDCKRNFFKC