AlfalfaGEDB | MS.gene21937

M. sativa cultivar XinJiangDaYe / MS.gene21937

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21937.t1	XP_003610946.1	97.8	551	12	0	1	551	1	551	4.6e-310	1073.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21937.t1	F4HUK6	66.1	549	182	1	1	545	3	551	3.5e-217	755.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21937.t1	G7K979	97.8	551	12	0	1	551	1	551	3.3e-310	1073.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050987	MS.gene21937	0.803139	4.02E-49	-1.69E-46
MS.gene054930	MS.gene21937	0.870597	1.29E-66	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene037815	MS.gene21937	PPI
MS.gene23786	MS.gene21937	PPI
MS.gene041844	MS.gene21937	PPI
MS.gene21937	MS.gene26216	PPI
MS.gene21937	MS.gene061174	PPI
MS.gene017547	MS.gene21937	PPI
MS.gene23782	MS.gene21937	PPI
MS.gene21937	MS.gene037813	PPI
MS.gene21937	MS.gene017279	PPI
MS.gene05028	MS.gene21937	PPI
MS.gene009614	MS.gene21937	PPI
MS.gene21937	MS.gene98148	PPI
MS.gene21937	MS.gene99857	PPI
MS.gene21937	MS.gene041843	PPI
MS.gene21937	MS.gene34290	PPI
MS.gene99100	MS.gene21937	PPI
MS.gene21937	MS.gene034275	PPI
MS.gene83513	MS.gene21937	PPI
MS.gene008350	MS.gene21937	PPI
MS.gene025344	MS.gene21937	PPI
MS.gene21937	MS.gene061578	PPI
MS.gene061175	MS.gene21937	PPI
MS.gene037817	MS.gene21937	PPI
MS.gene27218	MS.gene21937	PPI
MS.gene017552	MS.gene21937	PPI
MS.gene21937	MS.gene27213	PPI
MS.gene017545	MS.gene21937	PPI
MS.gene23788	MS.gene21937	PPI
MS.gene21937	MS.gene049681	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21937.t1	MTR_5g008800	98.367	551	9	0	1	551	1	551	0.0	1122
MS.gene21937.t1	MTR_5g008820	81.136	546	101	1	1	546	1	544	0.0	938
MS.gene21937.t1	MTR_8g074040	64.154	544	193	2	1	542	43	586	0.0	772
MS.gene21937.t1	MTR_8g074040	64.957	468	162	2	77	542	1	468	0.0	670
MS.gene21937.t1	MTR_8g074120	64.509	479	168	1	67	543	20	498	0.0	667
MS.gene21937.t1	MTR_4g121600	52.087	551	251	4	9	546	15	565	0.0	595
MS.gene21937.t1	MTR_2g044180	51.906	551	252	4	9	546	15	565	0.0	594
MS.gene21937.t1	MTR_3g064420	50.452	553	260	3	1	539	1	553	0.0	585
MS.gene21937.t1	MTR_5g076240	53.370	549	240	7	8	544	8	552	0.0	584
MS.gene21937.t1	MTR_5g076220	50.903	554	255	8	1	541	1	550	0.0	535
MS.gene21937.t1	MTR_5g076200	50.540	556	258	8	1	543	1	552	0.0	530
MS.gene21937.t1	MTR_5g023730	49.360	547	264	9	2	540	3	544	0.0	527
MS.gene21937.t1	MTR_0161s0050	50.179	560	256	8	1	543	1	554	2.59e-179	517
MS.gene21937.t1	MTR_2g044180	51.688	474	216	4	86	546	1	474	1.88e-175	504
MS.gene21937.t1	MTR_0803s0020	31.558	507	320	11	29	528	250	736	3.15e-63	224
MS.gene21937.t1	MTR_2g095510	29.135	532	319	16	29	538	42	537	5.41e-47	172
MS.gene21937.t1	MTR_8g039720	28.516	512	310	16	40	526	65	545	4.93e-46	170
MS.gene21937.t1	MTR_4g057730	28.440	545	339	14	14	538	27	540	8.14e-43	160
MS.gene21937.t1	MTR_1569s0010	26.667	540	333	11	32	533	14	528	1.91e-41	157
MS.gene21937.t1	MTR_1g026310	26.453	499	335	12	40	526	68	546	2.00e-41	157
MS.gene21937.t1	MTR_2g105570	27.930	512	340	14	39	538	53	547	2.07e-40	154
MS.gene21937.t1	MTR_3g035130	29.134	508	315	19	41	534	33	509	2.83e-40	153
MS.gene21937.t1	MTR_5g007640	27.878	556	339	17	9	538	66	585	1.04e-39	152
MS.gene21937.t1	MTR_3g460770	26.079	533	349	16	19	535	33	536	1.49e-38	149
MS.gene21937.t1	MTR_4g005750	28.767	511	329	14	40	538	53	540	3.82e-38	147
MS.gene21937.t1	MTR_3g088880	28.571	406	261	11	152	541	165	557	2.40e-37	145
MS.gene21937.t1	MTR_3g088870	28.610	374	242	8	180	541	199	559	1.53e-36	143
MS.gene21937.t1	MTR_1g090737	26.565	527	332	15	36	542	79	570	1.07e-35	141
MS.gene21937.t1	MTR_4g128337	26.834	518	334	16	27	526	44	534	1.02e-34	138
MS.gene21937.t1	MTR_1g073180	27.976	504	318	12	41	535	62	529	1.08e-33	135
MS.gene21937.t1	MTR_4g077900	27.655	499	316	14	52	533	1	471	1.32e-33	134
MS.gene21937.t1	MTR_5g027500	28.628	503	318	14	52	534	81	562	4.99e-33	133
MS.gene21937.t1	MTR_1g063110	26.629	537	330	15	39	551	55	551	1.01e-32	132
MS.gene21937.t1	MTR_1g063120	27.112	509	321	15	41	526	57	538	3.06e-32	130
MS.gene21937.t1	MTR_5g027480	29.518	498	306	17	52	533	80	548	3.12e-32	130
MS.gene21937.t1	MTR_3g035130	28.166	458	287	16	41	486	33	460	4.04e-31	126
MS.gene21937.t1	MTR_4g057730	27.789	475	293	13	14	469	27	470	7.11e-31	125
MS.gene21937.t1	MTR_2g030240	25.660	530	346	16	47	542	35	550	5.74e-30	124
MS.gene21937.t1	MTR_1g073180	27.273	484	308	11	41	516	84	531	6.15e-30	124
MS.gene21937.t1	MTR_2g095510	28.111	434	255	15	29	441	42	439	8.04e-28	116
MS.gene21937.t1	MTR_1586s0010	27.635	351	237	9	51	394	81	421	7.42e-26	110
MS.gene21937.t1	MTR_4g057730	26.696	457	287	13	14	452	27	453	1.91e-25	109
MS.gene21937.t1	MTR_2g030240	26.570	414	265	14	163	542	48	456	6.04e-25	108
MS.gene21937.t1	MTR_4g011790	27.013	385	231	13	182	534	227	593	1.87e-22	101
MS.gene21937.t1	MTR_4g011790	26.632	383	231	13	182	532	227	591	1.08e-21	99.4
MS.gene21937.t1	MTR_4g011790	26.396	394	231	13	182	534	227	602	2.42e-21	98.2
MS.gene21937.t1	MTR_4g013210	27.518	407	237	15	168	534	52	440	1.07e-20	95.5
MS.gene21937.t1	MTR_1054s0020	34.615	156	98	2	388	539	616	771	3.95e-20	94.7
MS.gene21937.t1	MTR_7g109830	23.636	495	290	18	41	470	78	549	1.96e-19	92.4
MS.gene21937.t1	MTR_1g086600	21.881	489	308	12	41	470	100	573	1.23e-18	90.1
MS.gene21937.t1	MTR_4g013210	27.089	395	213	16	168	534	52	399	1.02e-17	85.9
MS.gene21937.t1	MTR_4g013210	26.582	395	215	15	168	534	204	551	2.19e-17	85.9
MS.gene21937.t1	MTR_4g013210	27.168	346	205	11	168	484	204	531	3.89e-17	85.1
MS.gene21937.t1	MTR_4g068670	22.835	508	291	23	39	474	74	552	3.94e-17	85.1
MS.gene21937.t1	MTR_7g084850	25.139	541	344	20	37	536	200	720	6.09e-17	84.7
MS.gene21937.t1	MTR_1g026430	24.363	353	239	9	40	382	68	402	3.24e-14	75.1
MS.gene21937.t1	MTR_1g026470	24.011	354	241	9	40	383	68	403	2.75e-13	72.4
MS.gene21937.t1	MTR_3g087980	26.513	347	177	14	182	463	248	581	5.35e-13	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21937.t1	AT1G20560	68.488	549	169	1	1	545	3	551	0.0	806
MS.gene21937.t1	AT2G17650	60.971	556	203	2	1	542	48	603	0.0	719
MS.gene21937.t1	AT1G20560	68.710	473	144	1	77	545	1	473	0.0	694
MS.gene21937.t1	AT3G16910	51.268	552	255	4	9	546	17	568	0.0	601
MS.gene21937.t1	AT1G76290	53.407	543	245	4	1	540	1	538	0.0	597
MS.gene21937.t1	AT5G16370	51.543	551	252	7	1	541	1	546	0.0	570
MS.gene21937.t1	AT5G16340	50.909	550	255	6	1	540	1	545	0.0	560
MS.gene21937.t1	AT1G75960	51.184	549	253	6	1	539	1	544	0.0	550
MS.gene21937.t1	AT1G66120	49.453	548	268	4	1	539	1	548	0.0	548
MS.gene21937.t1	AT1G65880	48.381	556	270	4	1	539	1	556	0.0	541
MS.gene21937.t1	AT1G65890	48.468	555	270	4	1	539	1	555	0.0	536
MS.gene21937.t1	AT1G77240	48.723	548	268	7	1	539	1	544	0.0	535
MS.gene21937.t1	AT1G21530	47.645	552	272	8	1	539	1	548	0.0	525
MS.gene21937.t1	AT1G21530	47.645	552	270	9	1	539	1	546	1.49e-180	520
MS.gene21937.t1	AT1G21540	48.282	553	268	8	1	539	1	549	1.68e-180	520
MS.gene21937.t1	AT1G68270	44.144	555	264	8	1	539	1	525	3.11e-155	455
MS.gene21937.t1	AT4G05160	29.487	546	328	15	17	538	30	542	7.71e-50	180
MS.gene21937.t1	AT1G20510	26.838	544	350	16	7	526	11	530	1.61e-43	163
MS.gene21937.t1	AT1G20500	27.686	484	311	12	58	529	83	539	3.19e-43	162
MS.gene21937.t1	AT5G63380	31.436	369	226	11	179	536	202	554	8.72e-41	155
MS.gene21937.t1	AT3G21240	28.265	513	326	15	39	535	63	549	1.84e-40	154
MS.gene21937.t1	AT1G65060	26.874	547	346	15	10	535	46	559	6.84e-40	153
MS.gene21937.t1	AT1G20480	27.812	489	317	13	51	526	86	551	9.51e-40	152
MS.gene21937.t1	AT3G48990	27.841	528	333	13	19	534	17	508	2.52e-37	145
MS.gene21937.t1	AT3G21230	28.681	523	327	16	38	541	74	569	5.14e-35	139
MS.gene21937.t1	AT4G19010	29.810	369	226	10	182	534	205	556	1.25e-33	135
MS.gene21937.t1	AT1G20510	26.337	486	311	15	7	469	11	472	2.18e-33	133
MS.gene21937.t1	AT5G38120	27.216	485	315	13	56	529	82	539	3.73e-33	133
MS.gene21937.t1	AT3G21230	28.629	496	306	16	38	512	74	542	9.67e-31	126
MS.gene21937.t1	AT1G62940	26.938	516	321	19	41	535	55	535	9.88e-30	123
MS.gene21937.t1	AT3G21240	27.578	446	282	14	40	470	64	483	4.44e-29	120
MS.gene21937.t1	AT3G21240	27.578	446	282	14	40	470	64	483	4.44e-29	120
MS.gene21937.t1	AT5G38120	26.739	460	300	12	56	505	82	514	4.68e-29	121
MS.gene21937.t1	AT1G51680	26.628	522	328	15	39	535	65	556	1.31e-28	120
MS.gene21937.t1	AT1G20510	25.983	458	292	15	7	441	11	444	6.88e-27	114
MS.gene21937.t1	AT1G51680	26.680	491	306	14	39	505	65	525	3.85e-26	112
MS.gene21937.t1	AT1G30520	23.757	543	368	13	20	529	13	542	9.39e-25	108
MS.gene21937.t1	AT1G30520	23.757	543	368	13	20	529	13	542	9.39e-25	108
MS.gene21937.t1	AT3G21230	28.381	451	278	15	38	470	74	497	8.19e-24	105
MS.gene21937.t1	AT1G65060	25.442	452	284	14	10	441	46	464	8.49e-23	102
MS.gene21937.t1	AT3G16170	25.700	393	227	12	182	534	235	602	1.51e-22	102
MS.gene21937.t1	AT5G38120	26.179	424	276	12	56	469	82	478	6.10e-22	99.4
MS.gene21937.t1	AT5G38120	26.179	424	276	12	56	469	82	478	6.10e-22	99.4
MS.gene21937.t1	AT2G47240	23.673	490	296	16	41	470	78	549	4.97e-21	97.4
MS.gene21937.t1	AT2G47240	23.673	490	296	16	41	470	78	549	4.97e-21	97.4
MS.gene21937.t1	AT2G47240	23.673	490	296	16	41	470	78	549	4.97e-21	97.4
MS.gene21937.t1	AT2G47240	23.673	490	296	16	41	470	19	490	5.95e-21	97.1
MS.gene21937.t1	AT1G51680	26.316	456	282	14	39	470	65	490	1.30e-19	92.4
MS.gene21937.t1	AT1G20490	25.806	372	240	11	58	418	83	429	1.16e-18	89.4
MS.gene21937.t1	AT1G20490	25.806	372	240	11	58	418	83	429	1.16e-18	89.4
MS.gene21937.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene21937.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene21937.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene21937.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene21937.t1	AT1G49430	23.952	501	295	19	40	474	77	557	1.79e-17	86.3
MS.gene21937.t1	AT5G36880	23.818	550	340	21	37	536	195	715	2.57e-17	85.9
MS.gene21937.t1	AT1G64400	25.971	489	277	19	41	466	81	547	6.75e-17	84.7
MS.gene21937.t1	AT1G30520	22.128	470	320	11	20	465	13	460	1.70e-14	76.6
MS.gene21937.t1	AT1G30520	22.128	470	320	11	20	465	13	460	1.70e-14	76.6
MS.gene21937.t1	AT4G11030	23.108	502	275	21	41	466	81	547	8.27e-13	71.6

Find 136 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGAAGGATATTATCATATC+TGG	0.228485	5.1:-2798760	MS.gene21937:CDS
TCTCACTCCAATCAGCTTCT+TGG	0.231430	5.1:-2800418	MS.gene21937:CDS
TCATACATTGCTGGAATATT+AGG	0.253425	5.1:+2799855	None:intergenic
ATTGGATCCCATTCATCTTT+TGG	0.275746	5.1:+2799540	None:intergenic
CTTTAGCAGTTACATTTCTT+TGG	0.277220	5.1:+2799313	None:intergenic
GAAGTGTAATTCAAAGAAAT+TGG	0.280408	5.1:+2799522	None:intergenic
AACTACCAATCTTCCACTTT+TGG	0.288627	5.1:-2799664	MS.gene21937:CDS
AGTCTATGCCGGTGTATTTA+TGG	0.290959	5.1:-2799410	MS.gene21937:CDS
CCATGGGTGAGGTGATGTTT+AGG	0.293811	5.1:-2798912	MS.gene21937:CDS
CATTTAAAGGTGGATGGTTT+AGG	0.293868	5.1:-2798837	MS.gene21937:CDS
GCAACACAAGAGTCATTTAA+AGG	0.314268	5.1:-2798850	MS.gene21937:CDS
AGGTGCTTATCTTAATTCTT+TGG	0.316766	5.1:-2799457	MS.gene21937:CDS
CGAGACAGGCTACTCCATTC+TGG	0.326334	5.1:+2799045	None:intergenic
TATGGTTTGACAGAAACTTA+TGG	0.346969	5.1:-2799093	MS.gene21937:CDS
TGGGAGACCTGATGAGTATT+GGG	0.357069	5.1:-2798662	MS.gene21937:CDS
TACTCGTCATGATTCGTTGA+TGG	0.363124	5.1:-2799784	MS.gene21937:CDS
TTTGATTTAGTTCATTCATA+TGG	0.364655	5.1:-2799111	MS.gene21937:CDS
GTAATTCATGATCTACAAAA+AGG	0.367573	5.1:+2799727	None:intergenic
GATATCAGAAAGCCACTTCC+GGG	0.373117	5.1:-2799204	MS.gene21937:CDS
GCAATGTATGAGCTGCATTT+TGG	0.374424	5.1:-2799843	MS.gene21937:CDS
TGATATCAGAAAGCCACTTC+CGG	0.382311	5.1:-2799205	MS.gene21937:CDS
AATTAAGATAAGCACCTCTA+TGG	0.382407	5.1:+2799463	None:intergenic
CATGGGTGAGGTGATGTTTA+GGG	0.382589	5.1:-2798911	MS.gene21937:CDS
GTCTTGCCGGTTGATGTCTT+TGG	0.393924	5.1:+2798511	None:intergenic
TGGCTATATACTACACCTTT+AGG	0.394178	5.1:+2799483	None:intergenic
CAAAGAAATGTAACTGCTAA+AGG	0.396156	5.1:-2799312	MS.gene21937:CDS
ATTTCTTTGAATTACACTTC+AGG	0.409528	5.1:-2799519	MS.gene21937:CDS
TGACGGTTACATAGAACTTA+AGG	0.410660	5.1:-2798788	MS.gene21937:CDS
ATGGTTTAGGACTGGTGACT+TGG	0.411295	5.1:-2798824	MS.gene21937:CDS
TCCAAAATTAAGACCAAAAG+TGG	0.416587	5.1:+2799651	None:intergenic
GGAAGTGGCTTTCTGATATC+AGG	0.427800	5.1:+2799207	None:intergenic
ATTCTCATAGATCAATGTTC+CGG	0.429813	5.1:+2799602	None:intergenic
GTTGTTGAGGCAGCTGTTGT+TGG	0.430997	5.1:-2798682	MS.gene21937:CDS
TGAAAAGACCAAAAGATGAA+TGG	0.437562	5.1:-2799548	MS.gene21937:CDS
CGAATCATGACGAGTATTCA+AGG	0.441411	5.1:+2799791	None:intergenic
TTCTCATAGATCAATGTTCC+GGG	0.441818	5.1:+2799603	None:intergenic
AAATGCAGCTCATACATTGC+TGG	0.444160	5.1:+2799846	None:intergenic
TCGCCTGCTCCTCTCGAGAC+AGG	0.444470	5.1:+2799031	None:intergenic
CTCAAAATCAAGTTTCCCTT+CGG	0.445425	5.1:+2799572	None:intergenic
GAAGGAGAAGGCAAAGGCTA+TGG	0.446378	5.1:-2798473	MS.gene21937:CDS
GAAAAGACCAAAAGATGAAT+GGG	0.450929	5.1:-2799547	MS.gene21937:CDS
GAGCGCTCCAAGAAGCTGAT+TGG	0.451334	5.1:+2800411	None:intergenic
TAGCCTGTCTCGAGAGGAGC+AGG	0.452101	5.1:-2799034	MS.gene21937:CDS
CCATGTTTCATTGCAATGGA+TGG	0.452299	5.1:-2799380	MS.gene21937:CDS
GTCTCTCCCCAATACTCATC+AGG	0.454603	5.1:+2798655	None:intergenic
TAAGACCAAAAGTGGAAGAT+TGG	0.454652	5.1:+2799659	None:intergenic
CGAAGGATATTATCATATCT+GGG	0.456932	5.1:-2798759	MS.gene21937:CDS
GCAATGGATGGTGTCTTCCT+TGG	0.459486	5.1:-2799368	MS.gene21937:CDS
AAAGAAATGTAACTGCTAAA+GGG	0.463695	5.1:-2799311	MS.gene21937:CDS
ATTTGTGTAGGTTGCTGTGT+TGG	0.466354	5.1:-2799880	MS.gene21937:intron
TTGTTGAGGCAGCTGTTGTT+GGG	0.470119	5.1:-2798681	MS.gene21937:CDS
ACAGCTGCCTCAACAACTGC+TGG	0.470476	5.1:+2798688	None:intergenic
CGTCAAGGAGTACAACATAT+TGG	0.470912	5.1:-2798997	MS.gene21937:CDS
GTTCCCATGTTTCATTGCAA+TGG	0.474688	5.1:-2799384	MS.gene21937:CDS
ATTAATGAGTTCAGAACTGT+TGG	0.476604	5.1:+2799240	None:intergenic
AGAAACACCGAGTTGAGAAA+TGG	0.477163	5.1:+2800293	None:intergenic
GATCTGCCAAAGACATCAAC+CGG	0.477410	5.1:-2798517	MS.gene21937:CDS
TTGACAGAAACTTATGGTCC+TGG	0.482770	5.1:-2799087	MS.gene21937:CDS
AAAGGTGGATGGTTTAGGAC+TGG	0.483012	5.1:-2798832	MS.gene21937:CDS
CGGTGTTTCTCCTCATGATG+TGG	0.485617	5.1:-2800280	MS.gene21937:intron
TTGGGAGACCTGATGAGTAT+TGG	0.488351	5.1:-2798663	MS.gene21937:CDS
TTCCATGTGCAAATTGATCC+AGG	0.491293	5.1:+2799069	None:intergenic
CCATCCATTGCAATGAAACA+TGG	0.496766	5.1:+2799380	None:intergenic
GAACGTAGTTAGCGTTGCAT+TGG	0.497591	5.1:+2800442	None:intergenic
TGAATTACTTGATATTGCTA+AGG	0.502727	5.1:-2799712	MS.gene21937:CDS
ACTACTACCTTCCCCGGAAG+TGG	0.505110	5.1:+2799192	None:intergenic
TGGTTTAGGACTGGTGACTT+GGG	0.510160	5.1:-2798823	MS.gene21937:CDS
AAACATTAGCACCATTGAGT+TGG	0.513653	5.1:-2798731	MS.gene21937:CDS
GACTTTGTTATTGTAAACAA+TGG	0.513822	5.1:+2800386	None:intergenic
TGTGATGAATGGATATCTAA+AGG	0.519167	5.1:-2798881	MS.gene21937:CDS
AGGCGGTGCTCCACCGCCAC+CGG	0.520554	5.1:-2799163	MS.gene21937:CDS
GCTTCTTCCATTTCTCAACT+CGG	0.522221	5.1:-2800300	MS.gene21937:CDS
AATAACACCTTCCAACTCAA+TGG	0.523579	5.1:+2798720	None:intergenic
GGAACAACATCAAGTCCTAA+AGG	0.525359	5.1:-2799498	MS.gene21937:CDS
TTTGCACATGGAAACCAGAA+TGG	0.525741	5.1:-2799059	MS.gene21937:CDS
AGGGGAAACACTGTGATGAA+TGG	0.526822	5.1:-2798892	MS.gene21937:CDS
CATAGAACAGCCCCTGACAT+TGG	0.528095	5.1:+2799816	None:intergenic
CCTTGGAGTATTGCTGCTCA+AGG	0.538223	5.1:-2799351	MS.gene21937:CDS
CTTTAGAAATGTATTACAGT+CGG	0.538466	5.1:+2798422	None:intergenic
GGTGACATCGTTGTAGATGA+TGG	0.538839	5.1:+2800362	None:intergenic
GCTCATCCATCAGTCTCTCC+CGG	0.539601	5.1:-2799621	MS.gene21937:CDS
CAGAAAGCCACTTCCGGGGA+AGG	0.540398	5.1:-2799199	MS.gene21937:CDS
GTCATCACTACTACCTTCCC+CGG	0.545022	5.1:+2799186	None:intergenic
AAGAGTCATTTAAAGGTGGA+TGG	0.546163	5.1:-2798843	MS.gene21937:CDS
GAATTACTTGATATTGCTAA+GGG	0.549278	5.1:-2799711	MS.gene21937:CDS
AATGTTCCGGGAGAGACTGA+TGG	0.553323	5.1:+2799615	None:intergenic
TATGAGAATCTTATATCCGA+AGG	0.561953	5.1:-2799588	MS.gene21937:CDS
GGTACACTCTTCATTGTGAC+AGG	0.562785	5.1:+2798949	None:intergenic
TTGTGCGTTTGTGAAGTTGA+AGG	0.563096	5.1:-2798629	MS.gene21937:CDS
AAGGAGAAGGCAAAGGCTAT+GGG	0.567730	5.1:-2798472	MS.gene21937:CDS
AGTTAGAAGGTGACGATGGA+AGG	0.571786	5.1:-2800471	None:intergenic
CAATGAGATGAAGTCTATGC+CGG	0.575034	5.1:-2799421	MS.gene21937:CDS
CCTAAACATCACCTCACCCA+TGG	0.581531	5.1:+2798912	None:intergenic
GAAGGGAGGGCACAATGCTA+CGG	0.582366	5.1:-2798611	MS.gene21937:CDS
TTTGTTATTGTAAACAATGG+CGG	0.586498	5.1:+2800389	None:intergenic
GGGAAGGTAGTAGTGATGAC+AGG	0.592219	5.1:-2799183	MS.gene21937:CDS
AGGAGTACAACATATTGGAC+TGG	0.595988	5.1:-2798992	MS.gene21937:CDS
TGTGCGTTTGTGAAGTTGAA+GGG	0.597382	5.1:-2798628	MS.gene21937:CDS
AAGAAGCTGATTGGAGTGAG+AGG	0.599442	5.1:+2800420	None:intergenic
GTACAACATATTGGACTGGA+AGG	0.601901	5.1:-2798988	MS.gene21937:CDS
TAGTCATCCAGCAGTTGTTG+AGG	0.610515	5.1:-2798695	MS.gene21937:CDS
AAGCACAAAGTAACACACAC+GGG	0.611637	5.1:-2799270	MS.gene21937:CDS
ATTAGCACCATTGAGTTGGA+AGG	0.615739	5.1:-2798727	MS.gene21937:CDS
CATCCATTGCAATGAAACAT+GGG	0.616932	5.1:+2799381	None:intergenic
AAGGAACAAACCACATCATG+AGG	0.618013	5.1:+2800270	None:intergenic
ATGGAGTAGCCTGTCTCGAG+AGG	0.618262	5.1:-2799040	MS.gene21937:CDS
TTCAACTTCACAAACGCACA+AGG	0.624923	5.1:+2798631	None:intergenic
TGGAGTATTGCTGCTCAAGG+TGG	0.625763	5.1:-2799348	MS.gene21937:CDS
GAAGGATATTATCATATCTG+GGG	0.626950	5.1:-2798758	MS.gene21937:CDS
ATGAGAATCTTATATCCGAA+GGG	0.627897	5.1:-2799587	MS.gene21937:CDS
ATGGGTGAGGTGATGTTTAG+GGG	0.630548	5.1:-2798910	MS.gene21937:CDS
TGTCACAATGAAGAGTGTAC+CGG	0.633282	5.1:-2798947	MS.gene21937:CDS
AGAACTTAAGGATCGGTCGA+AGG	0.636389	5.1:-2798776	MS.gene21937:CDS
CGTTTGTGAAGTTGAAGGGA+GGG	0.637801	5.1:-2798624	MS.gene21937:CDS
TTGGGAGTAAAGCATTCTGA+CGG	0.644221	5.1:-2798805	MS.gene21937:CDS
CCGGCTGATGCGAAAACCAT+GGG	0.646339	5.1:-2798928	MS.gene21937:CDS
ACACAAGAGTCATTTAAAGG+TGG	0.651781	5.1:-2798847	MS.gene21937:CDS
GGAACACACCATAAATACAC+CGG	0.652672	5.1:+2799402	None:intergenic
CCTTGAGCAGCAATACTCCA+AGG	0.654172	5.1:+2799351	None:intergenic
GTCCTGGATCAATTTGCACA+TGG	0.656520	5.1:-2799071	MS.gene21937:CDS
TAAGCACAAAGTAACACACA+CGG	0.660502	5.1:-2799271	MS.gene21937:CDS
AGTGGCTTTCTGATATCAGG+TGG	0.661622	5.1:+2799210	None:intergenic
GGTGTAGTATATAGCCATAG+AGG	0.662140	5.1:-2799477	MS.gene21937:CDS
TGATGCGAAAACCATGGGTG+AGG	0.664777	5.1:-2798923	MS.gene21937:CDS
GTTACATAGAACTTAAGGAT+CGG	0.668885	5.1:-2798783	MS.gene21937:CDS
AAGGATATTATCATATCTGG+GGG	0.669368	5.1:-2798757	MS.gene21937:CDS
ATATCAGAAAGCCACTTCCG+GGG	0.673117	5.1:-2799203	MS.gene21937:CDS
ACCGGCTGATGCGAAAACCA+TGG	0.679532	5.1:-2798929	MS.gene21937:CDS
GCGTTTGTGAAGTTGAAGGG+AGG	0.681440	5.1:-2798625	MS.gene21937:CDS
ACAACGATGTCACCTACACA+TGG	0.682235	5.1:-2800353	MS.gene21937:CDS
AGCACAAAGTAACACACACG+GGG	0.696294	5.1:-2799269	MS.gene21937:CDS
GGGAGACCTGATGAGTATTG+GGG	0.710890	5.1:-2798661	MS.gene21937:CDS
AAGGTAGTAGTGATGACAGG+CGG	0.720822	5.1:-2799180	MS.gene21937:CDS
ATGAGTCTGAGACCATGTGT+AGG	0.729803	5.1:+2800341	None:intergenic
CACAAAGTAACACACACGGG+GGG	0.730025	5.1:-2799267	MS.gene21937:CDS
GCACAAAGTAACACACACGG+GGG	0.764468	5.1:-2799268	MS.gene21937:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTATTTTCTGTTAATTT+TGG	-	chr5.1:2798721-2798740	MS.gene21937:CDS	10.0%
!!!	AATTTGAATTTGTTTGAAAA+AGG	+	chr5.1:2798643-2798662	None:intergenic	15.0%
!!!	TTTGATTTAGTTCATTCATA+TGG	-	chr5.1:2799780-2799799	MS.gene21937:CDS	20.0%
!	AAAGAAATGTAACTGCTAAA+GGG	-	chr5.1:2799580-2799599	MS.gene21937:CDS	25.0%
!	ATTTCTTTGAATTACACTTC+AGG	-	chr5.1:2799372-2799391	MS.gene21937:CDS	25.0%
!	GAAGAAATATGATTACAAGT+TGG	-	chr5.1:2798941-2798960	MS.gene21937:CDS	25.0%
!	GAAGTGTAATTCAAAGAAAT+TGG	+	chr5.1:2799372-2799391	None:intergenic	25.0%
!	GAATTACTTGATATTGCTAA+GGG	-	chr5.1:2799180-2799199	MS.gene21937:CDS	25.0%
!	GTAATTCATGATCTACAAAA+AGG	+	chr5.1:2799167-2799186	None:intergenic	25.0%
!	TCGCTAGATTATAGATTTAA+AGG	+	chr5.1:2798683-2798702	None:intergenic	25.0%
!	TGAATTACTTGATATTGCTA+AGG	-	chr5.1:2799179-2799198	MS.gene21937:CDS	25.0%
!!	AAACTACTTAGAATTTTGCA+TGG	-	chr5.1:2798885-2798904	MS.gene21937:CDS	25.0%
!!!	AAAATATGTTTTGAAGGAGA+AGG	-	chr5.1:2800406-2800425	MS.gene21937:CDS	25.0%
!!!	GACACAAAAATATGTTTTGA+AGG	-	chr5.1:2800400-2800419	MS.gene21937:CDS	25.0%
!!!	GACTTTGTTATTGTAAACAA+TGG	+	chr5.1:2798508-2798527	None:intergenic	25.0%
!!!	TAATCTAGCGATTTTTCAAT+TGG	-	chr5.1:2798692-2798711	MS.gene21937:CDS	25.0%
!!!	TTAATTTTGGTTTTGATCGA+AGG	-	chr5.1:2798734-2798753	MS.gene21937:CDS	25.0%
!!!	TTTGTTATTGTAAACAATGG+CGG	+	chr5.1:2798505-2798524	None:intergenic	25.0%
	AAGGATATTATCATATCTGG+GGG	-	chr5.1:2800134-2800153	MS.gene21937:intron	30.0%
	AATTAAGATAAGCACCTCTA+TGG	+	chr5.1:2799431-2799450	None:intergenic	30.0%
	AGGTGCTTATCTTAATTCTT+TGG	-	chr5.1:2799434-2799453	MS.gene21937:CDS	30.0%
	ATGAGAATCTTATATCCGAA+GGG	-	chr5.1:2799304-2799323	MS.gene21937:CDS	30.0%
	ATTAATGAGTTCAGAACTGT+TGG	+	chr5.1:2799654-2799673	None:intergenic	30.0%
	ATTCTCATAGATCAATGTTC+CGG	+	chr5.1:2799292-2799311	None:intergenic	30.0%
	CAAAGAAATGTAACTGCTAA+AGG	-	chr5.1:2799579-2799598	MS.gene21937:CDS	30.0%
	CGAAGGATATTATCATATCT+GGG	-	chr5.1:2800132-2800151	MS.gene21937:intron	30.0%
	CTTTAGCAGTTACATTTCTT+TGG	+	chr5.1:2799581-2799600	None:intergenic	30.0%
	GAAAAGACCAAAAGATGAAT+GGG	-	chr5.1:2799344-2799363	MS.gene21937:CDS	30.0%
	GAAGGATATTATCATATCTG+GGG	-	chr5.1:2800133-2800152	MS.gene21937:intron	30.0%
	GAATTTGCACTTATTTGTGT+AGG	-	chr5.1:2798999-2799018	MS.gene21937:CDS	30.0%
	GATATTATAAAGCACAGACT+CGG	+	chr5.1:2798861-2798880	None:intergenic	30.0%
	GTTACATAGAACTTAAGGAT+CGG	-	chr5.1:2800108-2800127	MS.gene21937:intron	30.0%
	TATGAGAATCTTATATCCGA+AGG	-	chr5.1:2799303-2799322	MS.gene21937:CDS	30.0%
	TATGGTTTGACAGAAACTTA+TGG	-	chr5.1:2799798-2799817	MS.gene21937:CDS	30.0%
	TCATACATTGCTGGAATATT+AGG	+	chr5.1:2799039-2799058	None:intergenic	30.0%
	TCCAAAATTAAGACCAAAAG+TGG	+	chr5.1:2799243-2799262	None:intergenic	30.0%
	TCGAAGGATATTATCATATC+TGG	-	chr5.1:2800131-2800150	MS.gene21937:intron	30.0%
	TGAAAAGACCAAAAGATGAA+TGG	-	chr5.1:2799343-2799362	MS.gene21937:CDS	30.0%
	TGTGATGAATGGATATCTAA+AGG	-	chr5.1:2800010-2800029	MS.gene21937:intron	30.0%
!!	AACATATTTTTGTGTCTTGC+CGG	+	chr5.1:2800396-2800415	None:intergenic	30.0%
!!!	ACTTTTGGTCTTAATTTTGG+AGG	-	chr5.1:2799242-2799261	MS.gene21937:CDS	30.0%
!!!	ATTTTTTAGGATGGAAGAAC+TGG	-	chr5.1:2799755-2799774	MS.gene21937:CDS	30.0%
!!!	CTTTTGGTCTTAATTTTGGA+GGG	-	chr5.1:2799243-2799262	MS.gene21937:CDS	30.0%
!!!	TCCACTTTTGGTCTTAATTT+TGG	-	chr5.1:2799239-2799258	MS.gene21937:CDS	30.0%
!!!	TTTTTTAGGATGGAAGAACT+GGG	-	chr5.1:2799756-2799775	MS.gene21937:CDS	30.0%
	AAACATTAGCACCATTGAGT+TGG	-	chr5.1:2800160-2800179	MS.gene21937:intron	35.0%
	AAGAGTCATTTAAAGGTGGA+TGG	-	chr5.1:2800048-2800067	MS.gene21937:intron	35.0%
	AATAACACCTTCCAACTCAA+TGG	+	chr5.1:2800174-2800193	None:intergenic	35.0%
	ACACAAGAGTCATTTAAAGG+TGG	-	chr5.1:2800044-2800063	MS.gene21937:intron	35.0%
	CATCCATTGCAATGAAACAT+GGG	+	chr5.1:2799513-2799532	None:intergenic	35.0%
	CATTTAAAGGTGGATGGTTT+AGG	-	chr5.1:2800054-2800073	MS.gene21937:intron	35.0%
	CTCAAAATCAAGTTTCCCTT+CGG	+	chr5.1:2799322-2799341	None:intergenic	35.0%
	GCAACACAAGAGTCATTTAA+AGG	-	chr5.1:2800041-2800060	MS.gene21937:intron	35.0%
	TAAGACCAAAAGTGGAAGAT+TGG	+	chr5.1:2799235-2799254	None:intergenic	35.0%
	TAAGCACAAAGTAACACACA+CGG	-	chr5.1:2799620-2799639	MS.gene21937:CDS	35.0%
	TCACATATTCACATGTGTCT+AGG	+	chr5.1:2798791-2798810	None:intergenic	35.0%
	TCCATCCTAAAAAATACGTC+CGG	+	chr5.1:2799750-2799769	None:intergenic	35.0%
	TGACGGTTACATAGAACTTA+AGG	-	chr5.1:2800103-2800122	MS.gene21937:intron	35.0%
	TGGCTATATACTACACCTTT+AGG	+	chr5.1:2799411-2799430	None:intergenic	35.0%
	TTCTCATAGATCAATGTTCC+GGG	+	chr5.1:2799291-2799310	None:intergenic	35.0%
!	AACTACCAATCTTCCACTTT+TGG	-	chr5.1:2799227-2799246	MS.gene21937:CDS	35.0%
!	ATTGGATCCCATTCATCTTT+TGG	+	chr5.1:2799354-2799373	None:intergenic	35.0%
!	TACAAAAAGGATTTTCGCGT+CGG	+	chr5.1:2799154-2799173	None:intergenic	35.0%
!!!	ATTTTGGTGTTCCAATGTCA+GGG	-	chr5.1:2799064-2799083	MS.gene21937:CDS	35.0%
	AAATGCAGCTCATACATTGC+TGG	+	chr5.1:2799048-2799067	None:intergenic	40.0%
	AAGCACAAAGTAACACACAC+GGG	-	chr5.1:2799621-2799640	MS.gene21937:CDS	40.0%
	AAGGAACAAACCACATCATG+AGG	+	chr5.1:2798624-2798643	None:intergenic	40.0%
	AGTCTATGCCGGTGTATTTA+TGG	-	chr5.1:2799481-2799500	MS.gene21937:CDS	40.0%
	ATCCTAAAAAATACGTCCGG+TGG	+	chr5.1:2799747-2799766	None:intergenic	40.0%
	ATTTGTGTAGGTTGCTGTGT+TGG	-	chr5.1:2799011-2799030	MS.gene21937:CDS	40.0%
	CAATGAGATGAAGTCTATGC+CGG	-	chr5.1:2799470-2799489	MS.gene21937:CDS	40.0%
	CCATCCATTGCAATGAAACA+TGG	+	chr5.1:2799514-2799533	None:intergenic	40.0%
	CCATGTTTCATTGCAATGGA+TGG	-	chr5.1:2799511-2799530	MS.gene21937:CDS	40.0%
	CGAATCATGACGAGTATTCA+AGG	+	chr5.1:2799103-2799122	None:intergenic	40.0%
	GCTTCTTCCATTTCTCAACT+CGG	-	chr5.1:2798591-2798610	MS.gene21937:CDS	40.0%
	GGAACAACATCAAGTCCTAA+AGG	-	chr5.1:2799393-2799412	MS.gene21937:CDS	40.0%
	GGAACACACCATAAATACAC+CGG	+	chr5.1:2799492-2799511	None:intergenic	40.0%
	GGTGTAGTATATAGCCATAG+AGG	-	chr5.1:2799414-2799433	MS.gene21937:CDS	40.0%
	GTTCCCATGTTTCATTGCAA+TGG	-	chr5.1:2799507-2799526	MS.gene21937:CDS	40.0%
	TACTCGTCATGATTCGTTGA+TGG	-	chr5.1:2799107-2799126	MS.gene21937:CDS	40.0%
	TGATATCAGAAAGCCACTTC+CGG	-	chr5.1:2799686-2799705	MS.gene21937:CDS	40.0%
	TGTGCGTTTGTGAAGTTGAA+GGG	-	chr5.1:2800263-2800282	MS.gene21937:intron	40.0%
	TTCAACTTCACAAACGCACA+AGG	+	chr5.1:2800263-2800282	None:intergenic	40.0%
	TTCCATGTGCAAATTGATCC+AGG	+	chr5.1:2799825-2799844	None:intergenic	40.0%
	TTGACAGAAACTTATGGTCC+TGG	-	chr5.1:2799804-2799823	MS.gene21937:CDS	40.0%
	TTGTGCGTTTGTGAAGTTGA+AGG	-	chr5.1:2800262-2800281	MS.gene21937:intron	40.0%
	TTTGCACATGGAAACCAGAA+TGG	-	chr5.1:2799832-2799851	MS.gene21937:CDS	40.0%
!	AGAAACACCGAGTTGAGAAA+TGG	+	chr5.1:2798601-2798620	None:intergenic	40.0%
!	AGGAGTACAACATATTGGAC+TGG	-	chr5.1:2799899-2799918	MS.gene21937:intron	40.0%
!	CGAAGGGAAACTTGATTTTG+AGG	-	chr5.1:2799320-2799339	MS.gene21937:CDS	40.0%
!	CGTCAAGGAGTACAACATAT+TGG	-	chr5.1:2799894-2799913	MS.gene21937:intron	40.0%
!	GCAATGTATGAGCTGCATTT+TGG	-	chr5.1:2799048-2799067	MS.gene21937:CDS	40.0%
!	GTACAACATATTGGACTGGA+AGG	-	chr5.1:2799903-2799922	MS.gene21937:intron	40.0%
!	TCAGCAAACACGACAGTTTT+TGG	+	chr5.1:2800356-2800375	None:intergenic	40.0%
!	TGTCACAATGAAGAGTGTAC+CGG	-	chr5.1:2799944-2799963	MS.gene21937:intron	40.0%
!	TTGGGAGTAAAGCATTCTGA+CGG	-	chr5.1:2800086-2800105	MS.gene21937:intron	40.0%
!!	AAAGCGTTTGCCTCGTTTTA+TGG	-	chr5.1:2800328-2800347	MS.gene21937:CDS	40.0%
!!	ATTAGCACCATTGAGTTGGA+AGG	-	chr5.1:2800164-2800183	MS.gene21937:intron	40.0%
!!!	ACCGGACGTATTTTTTAGGA+TGG	-	chr5.1:2799746-2799765	MS.gene21937:CDS	40.0%
!!!	CATTTTGGTGTTCCAATGTC+AGG	-	chr5.1:2799063-2799082	MS.gene21937:CDS	40.0%
!!!	GTTTTTGGAGCCATAAAACG+AGG	+	chr5.1:2800341-2800360	None:intergenic	40.0%
!!!	TGTTTTGAAGGAGAAGGCAA+AGG	-	chr5.1:2800412-2800431	MS.gene21937:CDS	40.0%
!!!	TTTTGGTGTTCCAATGTCAG+GGG	-	chr5.1:2799065-2799084	MS.gene21937:CDS	40.0%
	AAGAAGCTGATTGGAGTGAG+AGG	+	chr5.1:2798474-2798493	None:intergenic	45.0%
	AAGGAGAAGGCAAAGGCTAT+GGG	-	chr5.1:2800419-2800438	MS.gene21937:CDS	45.0%
	AAGGTAGTAGTGATGACAGG+CGG	-	chr5.1:2799711-2799730	MS.gene21937:CDS	45.0%
	ACAACGATGTCACCTACACA+TGG	-	chr5.1:2798538-2798557	MS.gene21937:CDS	45.0%
	AGAACTTAAGGATCGGTCGA+AGG	-	chr5.1:2800115-2800134	MS.gene21937:intron	45.0%
	AGCACAAAGTAACACACACG+GGG	-	chr5.1:2799622-2799641	MS.gene21937:CDS	45.0%
	AGGGGAAACACTGTGATGAA+TGG	-	chr5.1:2799999-2800018	MS.gene21937:intron	45.0%
	AGTGGCTTTCTGATATCAGG+TGG	+	chr5.1:2799684-2799703	None:intergenic	45.0%
	ATATCAGAAAGCCACTTCCG+GGG	-	chr5.1:2799688-2799707	MS.gene21937:CDS	45.0%
	ATGGGTGAGGTGATGTTTAG+GGG	-	chr5.1:2799981-2800000	MS.gene21937:intron	45.0%
	CATGGGTGAGGTGATGTTTA+GGG	-	chr5.1:2799980-2799999	MS.gene21937:intron	45.0%
	CGTTTGTGAAGTTGAAGGGA+GGG	-	chr5.1:2800267-2800286	MS.gene21937:intron	45.0%
	CTAAAAAATACGTCCGGTGG+CGG	+	chr5.1:2799744-2799763	None:intergenic	45.0%
	GAACGTAGTTAGCGTTGCAT+TGG	+	chr5.1:2798452-2798471	None:intergenic	45.0%
	GATATCAGAAAGCCACTTCC+GGG	-	chr5.1:2799687-2799706	MS.gene21937:CDS	45.0%
	GATCTGCCAAAGACATCAAC+CGG	-	chr5.1:2800374-2800393	MS.gene21937:CDS	45.0%
	GCGAAAAAGAAGTCACGTCA+AGG	-	chr5.1:2799879-2799898	MS.gene21937:intron	45.0%
	GGAAGTGGCTTTCTGATATC+AGG	+	chr5.1:2799687-2799706	None:intergenic	45.0%
	GGTACACTCTTCATTGTGAC+AGG	+	chr5.1:2799945-2799964	None:intergenic	45.0%
	GGTGACATCGTTGTAGATGA+TGG	+	chr5.1:2798532-2798551	None:intergenic	45.0%
	GTCCTGGATCAATTTGCACA+TGG	-	chr5.1:2799820-2799839	MS.gene21937:CDS	45.0%
	TAGTCATCCAGCAGTTGTTG+AGG	-	chr5.1:2800196-2800215	MS.gene21937:intron	45.0%
	TCTCACTCCAATCAGCTTCT+TGG	-	chr5.1:2798473-2798492	MS.gene21937:CDS	45.0%
	TGGGAGACCTGATGAGTATT+GGG	-	chr5.1:2800229-2800248	MS.gene21937:intron	45.0%
	TTGGGAGACCTGATGAGTAT+TGG	-	chr5.1:2800228-2800247	MS.gene21937:intron	45.0%
	TTGTTGAGGCAGCTGTTGTT+GGG	-	chr5.1:2800210-2800229	MS.gene21937:intron	45.0%
!	AAAGGTGGATGGTTTAGGAC+TGG	-	chr5.1:2800059-2800078	MS.gene21937:intron	45.0%
!	ATGAGTCTGAGACCATGTGT+AGG	+	chr5.1:2798553-2798572	None:intergenic	45.0%
!	ATGGTTTAGGACTGGTGACT+TGG	-	chr5.1:2800067-2800086	MS.gene21937:intron	45.0%
!	TGGTTTAGGACTGGTGACTT+GGG	-	chr5.1:2800068-2800087	MS.gene21937:intron	45.0%
	AATGTTCCGGGAGAGACTGA+TGG	+	chr5.1:2799279-2799298	None:intergenic	50.0%
	CACAAAGTAACACACACGGG+GGG	-	chr5.1:2799624-2799643	MS.gene21937:CDS	50.0%
	CATAGAACAGCCCCTGACAT+TGG	+	chr5.1:2799078-2799097	None:intergenic	50.0%
	CCATGGGTGAGGTGATGTTT+AGG	-	chr5.1:2799979-2799998	MS.gene21937:intron	50.0%
	CCTAAACATCACCTCACCCA+TGG	+	chr5.1:2799982-2800001	None:intergenic	50.0%
	CCTTGAGCAGCAATACTCCA+AGG	+	chr5.1:2799543-2799562	None:intergenic	50.0%
	CGGTGTTTCTCCTCATGATG+TGG	-	chr5.1:2798611-2798630	MS.gene21937:CDS	50.0%
	GAAGGAGAAGGCAAAGGCTA+TGG	-	chr5.1:2800418-2800437	MS.gene21937:CDS	50.0%
	GCAATGGATGGTGTCTTCCT+TGG	-	chr5.1:2799523-2799542	MS.gene21937:CDS	50.0%
	GCACAAAGTAACACACACGG+GGG	-	chr5.1:2799623-2799642	MS.gene21937:CDS	50.0%
	GCGTTTGTGAAGTTGAAGGG+AGG	-	chr5.1:2800266-2800285	MS.gene21937:intron	50.0%
	GGGAAGGTAGTAGTGATGAC+AGG	-	chr5.1:2799708-2799727	MS.gene21937:CDS	50.0%
	GTATCCGACATGTGTCTGTC+TGG	-	chr5.1:2798765-2798784	MS.gene21937:CDS	50.0%
	GTCATCACTACTACCTTCCC+CGG	+	chr5.1:2799708-2799727	None:intergenic	50.0%
	GTCTCTCCCCAATACTCATC+AGG	+	chr5.1:2800239-2800258	None:intergenic	50.0%
	GTTGTTGAGGCAGCTGTTGT+TGG	-	chr5.1:2800209-2800228	MS.gene21937:intron	50.0%
	TAGGCCAGACAGACACATGT+CGG	+	chr5.1:2798772-2798791	None:intergenic	50.0%
	TGATGCGAAAACCATGGGTG+AGG	-	chr5.1:2799968-2799987	MS.gene21937:intron	50.0%
!	GGGAGACCTGATGAGTATTG+GGG	-	chr5.1:2800230-2800249	MS.gene21937:intron	50.0%
!	GTCTTGCCGGTTGATGTCTT+TGG	+	chr5.1:2800383-2800402	None:intergenic	50.0%
!!	CCTTGGAGTATTGCTGCTCA+AGG	-	chr5.1:2799540-2799559	MS.gene21937:CDS	50.0%
!!	TGGAGTATTGCTGCTCAAGG+TGG	-	chr5.1:2799543-2799562	MS.gene21937:CDS	50.0%
!!!	CGCCACCGGACGTATTTTTT+AGG	-	chr5.1:2799742-2799761	MS.gene21937:CDS	50.0%
	ACAGCTGCCTCAACAACTGC+TGG	+	chr5.1:2800206-2800225	None:intergenic	55.0%
	ACCGGCTGATGCGAAAACCA+TGG	-	chr5.1:2799962-2799981	MS.gene21937:intron	55.0%
	ACTACTACCTTCCCCGGAAG+TGG	+	chr5.1:2799702-2799721	None:intergenic	55.0%
	ATGGAGTAGCCTGTCTCGAG+AGG	-	chr5.1:2799851-2799870	MS.gene21937:CDS	55.0%
	CCGGCTGATGCGAAAACCAT+GGG	-	chr5.1:2799963-2799982	MS.gene21937:intron	55.0%
	CGAGACAGGCTACTCCATTC+TGG	+	chr5.1:2799849-2799868	None:intergenic	55.0%
	GAGCGCTCCAAGAAGCTGAT+TGG	+	chr5.1:2798483-2798502	None:intergenic	55.0%
	GCTCATCCATCAGTCTCTCC+CGG	-	chr5.1:2799270-2799289	MS.gene21937:CDS	55.0%
!	AAAAATACGTCCGGTGGCGG+TGG	+	chr5.1:2799741-2799760	None:intergenic	55.0%
!	CCCATGGTTTTCGCATCAGC+CGG	+	chr5.1:2799966-2799985	None:intergenic	55.0%
!	GAAGGGAGGGCACAATGCTA+CGG	-	chr5.1:2800280-2800299	MS.gene21937:intron	55.0%
	CAGAAAGCCACTTCCGGGGA+AGG	-	chr5.1:2799692-2799711	MS.gene21937:CDS	60.0%
	TAGCCTGTCTCGAGAGGAGC+AGG	-	chr5.1:2799857-2799876	MS.gene21937:CDS	60.0%
	TCGCCTGCTCCTCTCGAGAC+AGG	+	chr5.1:2799863-2799882	None:intergenic	65.0%
	AGGCGGTGCTCCACCGCCAC+CGG	-	chr5.1:2799728-2799747	MS.gene21937:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	2798435	2800478	2798435	ID=MS.gene21937
chr5.1	mRNA	2798435	2800478	2798435	ID=MS.gene21937.t1;Parent=MS.gene21937
chr5.1	exon	2800281	2800478	2800281	ID=MS.gene21937.t1.exon1;Parent=MS.gene21937.t1
chr5.1	CDS	2800281	2800478	2800281	ID=cds.MS.gene21937.t1;Parent=MS.gene21937.t1
chr5.1	exon	2798435	2799892	2798435	ID=MS.gene21937.t1.exon2;Parent=MS.gene21937.t1
chr5.1	CDS	2798435	2799892	2798435	ID=cds.MS.gene21937.t1;Parent=MS.gene21937.t1

Gene Sequence

>MS.gene21937

ATGGAAGGTACTATCCAATGCAACGCTAACTACGTTCCTCTCACTCCAATCAGCTTCTTGGAGCGCTCCGCCATTGTTTACAATAACAAAGTCTCCATCATCTACAACGATGTCACCTACACATGGTCTCAGACTCATCAACGATGTATCAAACTCGCTTCTTCCATTTCTCAACTCGGTGTTTCTCCTCATGATGTGGTTTGTTCCTTTTTCAAACAAATTCAAATTCAACATAGTTTAATTCTCCTTTAAATCTATAATCTAGCGATTTTTCAATTGGATTTGATTTTTATTTTCTGTTAATTTTGGTTTTGATCGAAGGCGTGTTCAGTATCCGACATGTGTCTGTCTGGCCTAGACACATGTGAATATGTGATGTATCACACTAAATTATGTCATTTTTTAAAATAATTATTGATGTGTCCGAGTCTGTGCTTTATAATATCAATCAAACTACTTAGAATTTTGCATGGAACATGTAAATATGATTATAATTTTTGATTTTAGAAGAAATATGATTACAAGTTGGTTTGAAATTTCTTGTTGATGATTTGTGCTTGTTGTGAATTTGCACTTATTTGTGTAGGTTGCTGTGTTGGCTCCTAATATTCCAGCAATGTATGAGCTGCATTTTGGTGTTCCAATGTCAGGGGCTGTTCTATGTACCTTGAATACTCGTCATGATTCGTTGATGGTTTCGTTGTTGTTAAAGCATTCCGACGCGAAAATCCTTTTTGTAGATCATGAATTACTTGATATTGCTAAGGGAGCACTTGAAATTTTGTCGAAATCAACTACCAATCTTCCACTTTTGGTCTTAATTTTGGAGGGTGATGCTCATCCATCAGTCTCTCCCGGAACATTGATCTATGAGAATCTTATATCCGAAGGGAAACTTGATTTTGAGGTGAAAAGACCAAAAGATGAATGGGATCCAATTTCTTTGAATTACACTTCAGGAACAACATCAAGTCCTAAAGGTGTAGTATATAGCCATAGAGGTGCTTATCTTAATTCTTTGGCTACAATTCTTCTCAATGAGATGAAGTCTATGCCGGTGTATTTATGGTGTGTTCCCATGTTTCATTGCAATGGATGGTGTCTTCCTTGGAGTATTGCTGCTCAAGGTGGCACAAATGTTTGCCAAAGAAATGTAACTGCTAAAGGGATATTTGACAATATTTTTAAGCACAAAGTAACACACACGGGGGGTGCACCAACAGTTCTGAACTCATTAATAAATGCACCACCTGATATCAGAAAGCCACTTCCGGGGAAGGTAGTAGTGATGACAGGCGGTGCTCCACCGCCACCGGACGTATTTTTTAGGATGGAAGAACTGGGATTTGATTTAGTTCATTCATATGGTTTGACAGAAACTTATGGTCCTGGATCAATTTGCACATGGAAACCAGAATGGAGTAGCCTGTCTCGAGAGGAGCAGGCGAAAAAGAAGTCACGTCAAGGAGTACAACATATTGGACTGGAAGGACTTGACATAAAAGATCCTGTCACAATGAAGAGTGTACCGGCTGATGCGAAAACCATGGGTGAGGTGATGTTTAGGGGAAACACTGTGATGAATGGATATCTAAAGGATTTAAAAGCAACACAAGAGTCATTTAAAGGTGGATGGTTTAGGACTGGTGACTTGGGAGTAAAGCATTCTGACGGTTACATAGAACTTAAGGATCGGTCGAAGGATATTATCATATCTGGGGGAGAAAACATTAGCACCATTGAGTTGGAAGGTGTTATTTATAGTCATCCAGCAGTTGTTGAGGCAGCTGTTGTTGGGAGACCTGATGAGTATTGGGGAGAGACACCTTGTGCGTTTGTGAAGTTGAAGGGAGGGCACAATGCTACGGAAGAAGAGATAATACAATTCTGTCAAAAGCGTTTGCCTCGTTTTATGGCTCCAAAAACTGTCGTGTTTGCTGATCTGCCAAAGACATCAACCGGCAAGACACAAAAATATGTTTTGAAGGAGAAGGCAAAGGCTATGGGAAGCTTGTCTAAGAAAAAGAATACAAGCCGACTGTAA

Protein sequence

>MS.gene21937.t1

MEGTIQCNANYVPLTPISFLERSAIVYNNKVSIIYNDVTYTWSQTHQRCIKLASSISQLGVSPHDVVAVLAPNIPAMYELHFGVPMSGAVLCTLNTRHDSLMVSLLLKHSDAKILFVDHELLDIAKGALEILSKSTTNLPLLVLILEGDAHPSVSPGTLIYENLISEGKLDFEVKRPKDEWDPISLNYTSGTTSSPKGVVYSHRGAYLNSLATILLNEMKSMPVYLWCVPMFHCNGWCLPWSIAAQGGTNVCQRNVTAKGIFDNIFKHKVTHTGGAPTVLNSLINAPPDIRKPLPGKVVVMTGGAPPPPDVFFRMEELGFDLVHSYGLTETYGPGSICTWKPEWSSLSREEQAKKKSRQGVQHIGLEGLDIKDPVTMKSVPADAKTMGEVMFRGNTVMNGYLKDLKATQESFKGGWFRTGDLGVKHSDGYIELKDRSKDIIISGGENISTIELEGVIYSHPAVVEAAVVGRPDEYWGETPCAFVKLKGGHNATEEEIIQFCQKRLPRFMAPKTVVFADLPKTSTGKTQKYVLKEKAKAMGSLSKKKNTSRL