AlfalfaGEDB | MS.gene22277

M. sativa cultivar XinJiangDaYe / MS.gene22277

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22277.t1	XP_003610946.1	97.8	551	12	0	1	551	1	551	4.6e-310	1073.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22277.t1	F4HUK6	66.1	549	182	1	1	545	3	551	3.5e-217	755.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22277.t1	G7K979	97.8	551	12	0	1	551	1	551	3.3e-310	1073.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054930	MS.gene22277	0.849839	2.50E-60	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene22277	MS.gene049681	PPI
MS.gene22277	MS.gene83513	PPI
MS.gene22277	MS.gene008350	PPI
MS.gene037817	MS.gene22277	PPI
MS.gene22277	MS.gene26216	PPI
MS.gene22277	MS.gene27213	PPI
MS.gene017552	MS.gene22277	PPI
MS.gene22277	MS.gene23788	PPI
MS.gene017545	MS.gene22277	PPI
MS.gene22277	MS.gene23786	PPI
MS.gene037815	MS.gene22277	PPI
MS.gene041844	MS.gene22277	PPI
MS.gene009614	MS.gene22277	PPI
MS.gene22277	MS.gene061174	PPI
MS.gene22277	MS.gene037813	PPI
MS.gene22277	MS.gene27218	PPI
MS.gene22277	MS.gene23782	PPI
MS.gene22277	MS.gene017279	PPI
MS.gene22277	MS.gene017547	PPI
MS.gene025344	MS.gene22277	PPI
MS.gene22277	MS.gene98148	PPI
MS.gene22277	MS.gene061578	PPI
MS.gene22277	MS.gene99857	PPI
MS.gene22277	MS.gene041843	PPI
MS.gene22277	MS.gene34290	PPI
MS.gene22277	MS.gene99100	PPI
MS.gene061175	MS.gene22277	PPI
MS.gene22277	MS.gene034275	PPI
MS.gene22277	MS.gene05028	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22277.t1	MTR_5g008800	98.367	551	9	0	1	551	1	551	0.0	1122
MS.gene22277.t1	MTR_5g008820	81.136	546	101	1	1	546	1	544	0.0	938
MS.gene22277.t1	MTR_8g074040	64.154	544	193	2	1	542	43	586	0.0	772
MS.gene22277.t1	MTR_8g074040	64.957	468	162	2	77	542	1	468	0.0	670
MS.gene22277.t1	MTR_8g074120	64.509	479	168	1	67	543	20	498	0.0	667
MS.gene22277.t1	MTR_4g121600	52.087	551	251	4	9	546	15	565	0.0	595
MS.gene22277.t1	MTR_2g044180	51.906	551	252	4	9	546	15	565	0.0	594
MS.gene22277.t1	MTR_3g064420	50.452	553	260	3	1	539	1	553	0.0	585
MS.gene22277.t1	MTR_5g076240	53.370	549	240	7	8	544	8	552	0.0	584
MS.gene22277.t1	MTR_5g076220	50.903	554	255	8	1	541	1	550	0.0	535
MS.gene22277.t1	MTR_5g076200	50.540	556	258	8	1	543	1	552	0.0	530
MS.gene22277.t1	MTR_5g023730	49.360	547	264	9	2	540	3	544	0.0	527
MS.gene22277.t1	MTR_0161s0050	50.179	560	256	8	1	543	1	554	2.59e-179	517
MS.gene22277.t1	MTR_2g044180	51.688	474	216	4	86	546	1	474	1.88e-175	504
MS.gene22277.t1	MTR_0803s0020	31.558	507	320	11	29	528	250	736	3.15e-63	224
MS.gene22277.t1	MTR_2g095510	29.135	532	319	16	29	538	42	537	5.41e-47	172
MS.gene22277.t1	MTR_8g039720	28.516	512	310	16	40	526	65	545	4.93e-46	170
MS.gene22277.t1	MTR_4g057730	28.440	545	339	14	14	538	27	540	8.14e-43	160
MS.gene22277.t1	MTR_1569s0010	26.667	540	333	11	32	533	14	528	1.91e-41	157
MS.gene22277.t1	MTR_1g026310	26.453	499	335	12	40	526	68	546	2.00e-41	157
MS.gene22277.t1	MTR_2g105570	27.930	512	340	14	39	538	53	547	2.07e-40	154
MS.gene22277.t1	MTR_3g035130	29.134	508	315	19	41	534	33	509	2.83e-40	153
MS.gene22277.t1	MTR_5g007640	27.878	556	339	17	9	538	66	585	1.04e-39	152
MS.gene22277.t1	MTR_3g460770	26.079	533	349	16	19	535	33	536	1.49e-38	149
MS.gene22277.t1	MTR_4g005750	28.767	511	329	14	40	538	53	540	3.82e-38	147
MS.gene22277.t1	MTR_3g088880	28.571	406	261	11	152	541	165	557	2.40e-37	145
MS.gene22277.t1	MTR_3g088870	28.610	374	242	8	180	541	199	559	1.53e-36	143
MS.gene22277.t1	MTR_1g090737	26.565	527	332	15	36	542	79	570	1.07e-35	141
MS.gene22277.t1	MTR_4g128337	26.834	518	334	16	27	526	44	534	1.02e-34	138
MS.gene22277.t1	MTR_1g073180	27.976	504	318	12	41	535	62	529	1.08e-33	135
MS.gene22277.t1	MTR_4g077900	27.655	499	316	14	52	533	1	471	1.32e-33	134
MS.gene22277.t1	MTR_5g027500	28.628	503	318	14	52	534	81	562	4.99e-33	133
MS.gene22277.t1	MTR_1g063110	26.629	537	330	15	39	551	55	551	1.01e-32	132
MS.gene22277.t1	MTR_1g063120	27.112	509	321	15	41	526	57	538	3.06e-32	130
MS.gene22277.t1	MTR_5g027480	29.518	498	306	17	52	533	80	548	3.12e-32	130
MS.gene22277.t1	MTR_3g035130	28.166	458	287	16	41	486	33	460	4.04e-31	126
MS.gene22277.t1	MTR_4g057730	27.789	475	293	13	14	469	27	470	7.11e-31	125
MS.gene22277.t1	MTR_2g030240	25.660	530	346	16	47	542	35	550	5.74e-30	124
MS.gene22277.t1	MTR_1g073180	27.273	484	308	11	41	516	84	531	6.15e-30	124
MS.gene22277.t1	MTR_2g095510	28.111	434	255	15	29	441	42	439	8.04e-28	116
MS.gene22277.t1	MTR_1586s0010	27.635	351	237	9	51	394	81	421	7.42e-26	110
MS.gene22277.t1	MTR_4g057730	26.696	457	287	13	14	452	27	453	1.91e-25	109
MS.gene22277.t1	MTR_2g030240	26.570	414	265	14	163	542	48	456	6.04e-25	108
MS.gene22277.t1	MTR_4g011790	27.013	385	231	13	182	534	227	593	1.87e-22	101
MS.gene22277.t1	MTR_4g011790	26.632	383	231	13	182	532	227	591	1.08e-21	99.4
MS.gene22277.t1	MTR_4g011790	26.396	394	231	13	182	534	227	602	2.42e-21	98.2
MS.gene22277.t1	MTR_4g013210	27.518	407	237	15	168	534	52	440	1.07e-20	95.5
MS.gene22277.t1	MTR_1054s0020	34.615	156	98	2	388	539	616	771	3.95e-20	94.7
MS.gene22277.t1	MTR_7g109830	23.636	495	290	18	41	470	78	549	1.96e-19	92.4
MS.gene22277.t1	MTR_1g086600	21.881	489	308	12	41	470	100	573	1.23e-18	90.1
MS.gene22277.t1	MTR_4g013210	27.089	395	213	16	168	534	52	399	1.02e-17	85.9
MS.gene22277.t1	MTR_4g013210	26.582	395	215	15	168	534	204	551	2.19e-17	85.9
MS.gene22277.t1	MTR_4g013210	27.168	346	205	11	168	484	204	531	3.89e-17	85.1
MS.gene22277.t1	MTR_4g068670	22.835	508	291	23	39	474	74	552	3.94e-17	85.1
MS.gene22277.t1	MTR_7g084850	25.139	541	344	20	37	536	200	720	6.09e-17	84.7
MS.gene22277.t1	MTR_1g026430	24.363	353	239	9	40	382	68	402	3.24e-14	75.1
MS.gene22277.t1	MTR_1g026470	24.011	354	241	9	40	383	68	403	2.75e-13	72.4
MS.gene22277.t1	MTR_3g087980	26.513	347	177	14	182	463	248	581	5.35e-13	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22277.t1	AT1G20560	68.488	549	169	1	1	545	3	551	0.0	806
MS.gene22277.t1	AT2G17650	60.971	556	203	2	1	542	48	603	0.0	719
MS.gene22277.t1	AT1G20560	68.710	473	144	1	77	545	1	473	0.0	694
MS.gene22277.t1	AT3G16910	51.268	552	255	4	9	546	17	568	0.0	601
MS.gene22277.t1	AT1G76290	53.407	543	245	4	1	540	1	538	0.0	597
MS.gene22277.t1	AT5G16370	51.543	551	252	7	1	541	1	546	0.0	570
MS.gene22277.t1	AT5G16340	50.909	550	255	6	1	540	1	545	0.0	560
MS.gene22277.t1	AT1G75960	51.184	549	253	6	1	539	1	544	0.0	550
MS.gene22277.t1	AT1G66120	49.453	548	268	4	1	539	1	548	0.0	548
MS.gene22277.t1	AT1G65880	48.381	556	270	4	1	539	1	556	0.0	541
MS.gene22277.t1	AT1G65890	48.468	555	270	4	1	539	1	555	0.0	536
MS.gene22277.t1	AT1G77240	48.723	548	268	7	1	539	1	544	0.0	535
MS.gene22277.t1	AT1G21530	47.645	552	272	8	1	539	1	548	0.0	525
MS.gene22277.t1	AT1G21530	47.645	552	270	9	1	539	1	546	1.49e-180	520
MS.gene22277.t1	AT1G21540	48.282	553	268	8	1	539	1	549	1.68e-180	520
MS.gene22277.t1	AT1G68270	44.144	555	264	8	1	539	1	525	3.11e-155	455
MS.gene22277.t1	AT4G05160	29.487	546	328	15	17	538	30	542	7.71e-50	180
MS.gene22277.t1	AT1G20510	26.838	544	350	16	7	526	11	530	1.61e-43	163
MS.gene22277.t1	AT1G20500	27.686	484	311	12	58	529	83	539	3.19e-43	162
MS.gene22277.t1	AT5G63380	31.436	369	226	11	179	536	202	554	8.72e-41	155
MS.gene22277.t1	AT3G21240	28.265	513	326	15	39	535	63	549	1.84e-40	154
MS.gene22277.t1	AT1G65060	26.874	547	346	15	10	535	46	559	6.84e-40	153
MS.gene22277.t1	AT1G20480	27.812	489	317	13	51	526	86	551	9.51e-40	152
MS.gene22277.t1	AT3G48990	27.841	528	333	13	19	534	17	508	2.52e-37	145
MS.gene22277.t1	AT3G21230	28.681	523	327	16	38	541	74	569	5.14e-35	139
MS.gene22277.t1	AT4G19010	29.810	369	226	10	182	534	205	556	1.25e-33	135
MS.gene22277.t1	AT1G20510	26.337	486	311	15	7	469	11	472	2.18e-33	133
MS.gene22277.t1	AT5G38120	27.216	485	315	13	56	529	82	539	3.73e-33	133
MS.gene22277.t1	AT3G21230	28.629	496	306	16	38	512	74	542	9.67e-31	126
MS.gene22277.t1	AT1G62940	26.938	516	321	19	41	535	55	535	9.88e-30	123
MS.gene22277.t1	AT3G21240	27.578	446	282	14	40	470	64	483	4.44e-29	120
MS.gene22277.t1	AT3G21240	27.578	446	282	14	40	470	64	483	4.44e-29	120
MS.gene22277.t1	AT5G38120	26.739	460	300	12	56	505	82	514	4.68e-29	121
MS.gene22277.t1	AT1G51680	26.628	522	328	15	39	535	65	556	1.31e-28	120
MS.gene22277.t1	AT1G20510	25.983	458	292	15	7	441	11	444	6.88e-27	114
MS.gene22277.t1	AT1G51680	26.680	491	306	14	39	505	65	525	3.85e-26	112
MS.gene22277.t1	AT1G30520	23.757	543	368	13	20	529	13	542	9.39e-25	108
MS.gene22277.t1	AT1G30520	23.757	543	368	13	20	529	13	542	9.39e-25	108
MS.gene22277.t1	AT3G21230	28.381	451	278	15	38	470	74	497	8.19e-24	105
MS.gene22277.t1	AT1G65060	25.442	452	284	14	10	441	46	464	8.49e-23	102
MS.gene22277.t1	AT3G16170	25.700	393	227	12	182	534	235	602	1.51e-22	102
MS.gene22277.t1	AT5G38120	26.179	424	276	12	56	469	82	478	6.10e-22	99.4
MS.gene22277.t1	AT5G38120	26.179	424	276	12	56	469	82	478	6.10e-22	99.4
MS.gene22277.t1	AT2G47240	23.673	490	296	16	41	470	78	549	4.97e-21	97.4
MS.gene22277.t1	AT2G47240	23.673	490	296	16	41	470	78	549	4.97e-21	97.4
MS.gene22277.t1	AT2G47240	23.673	490	296	16	41	470	78	549	4.97e-21	97.4
MS.gene22277.t1	AT2G47240	23.673	490	296	16	41	470	19	490	5.95e-21	97.1
MS.gene22277.t1	AT1G51680	26.316	456	282	14	39	470	65	490	1.30e-19	92.4
MS.gene22277.t1	AT1G20490	25.806	372	240	11	58	418	83	429	1.16e-18	89.4
MS.gene22277.t1	AT1G20490	25.806	372	240	11	58	418	83	429	1.16e-18	89.4
MS.gene22277.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene22277.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene22277.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene22277.t1	AT5G36880	23.497	549	343	20	37	536	145	665	1.77e-17	86.7
MS.gene22277.t1	AT1G49430	23.952	501	295	19	40	474	77	557	1.79e-17	86.3
MS.gene22277.t1	AT5G36880	23.818	550	340	21	37	536	195	715	2.57e-17	85.9
MS.gene22277.t1	AT1G64400	25.971	489	277	19	41	466	81	547	6.75e-17	84.7
MS.gene22277.t1	AT1G30520	22.128	470	320	11	20	465	13	460	1.70e-14	76.6
MS.gene22277.t1	AT1G30520	22.128	470	320	11	20	465	13	460	1.70e-14	76.6
MS.gene22277.t1	AT4G11030	23.108	502	275	21	41	466	81	547	8.27e-13	71.6

Find 137 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGAAGGATATTATCATATC+TGG	0.228485	5.3:-2958258	MS.gene22277:CDS
TCTCACTCCAATCAGCTTCT+TGG	0.231430	5.3:-2959916	MS.gene22277:CDS
TCATACATTGCTGGAATATT+AGG	0.253425	5.3:+2959353	None:intergenic
ATTGGATCCCATTCATCTTT+TGG	0.275746	5.3:+2959038	None:intergenic
CTTTAGCAGTTACATTTCTT+TGG	0.277220	5.3:+2958811	None:intergenic
GAAGTGTAATTCAAAGAAAT+TGG	0.280408	5.3:+2959020	None:intergenic
AACTACCAATCTTCCACTTT+TGG	0.288627	5.3:-2959162	MS.gene22277:CDS
AGTCTATGCCGGTGTATTTA+TGG	0.290959	5.3:-2958908	MS.gene22277:CDS
CCATGGGTGAGGTGATGTTT+AGG	0.293811	5.3:-2958410	MS.gene22277:CDS
CATTTAAAGGTGGATGGTTT+AGG	0.293868	5.3:-2958335	MS.gene22277:CDS
GCAACACAAGAGTCATTTAA+AGG	0.314268	5.3:-2958348	MS.gene22277:CDS
AGGTGCTTATCTTAATTCTT+TGG	0.316766	5.3:-2958955	MS.gene22277:CDS
CGAGACAGGCTACTCCATTC+TGG	0.326334	5.3:+2958543	None:intergenic
TATGGTTTGACAGAAACTTA+TGG	0.346969	5.3:-2958591	MS.gene22277:CDS
TGGGAGACCTGATGAGTATT+GGG	0.357069	5.3:-2958160	MS.gene22277:CDS
TACTCGTCATGATTCGTTGA+TGG	0.363124	5.3:-2959282	MS.gene22277:CDS
TTTGATTTAGTTCATTCATA+TGG	0.364655	5.3:-2958609	MS.gene22277:CDS
GTAATTCATGATCTACAAAA+AGG	0.367573	5.3:+2959225	None:intergenic
GATATCAGAAAGCCACTTCC+GGG	0.373117	5.3:-2958702	MS.gene22277:CDS
GCAATGTATGAGCTGCATTT+TGG	0.374424	5.3:-2959341	MS.gene22277:CDS
TGATATCAGAAAGCCACTTC+CGG	0.382311	5.3:-2958703	MS.gene22277:CDS
AATTAAGATAAGCACCTCTA+TGG	0.382407	5.3:+2958961	None:intergenic
CATGGGTGAGGTGATGTTTA+GGG	0.382589	5.3:-2958409	MS.gene22277:CDS
GTCTTGCCGGTTGATGTCTT+TGG	0.393924	5.3:+2958009	None:intergenic
TGGCTATATACTACACCTTT+AGG	0.394178	5.3:+2958981	None:intergenic
CAAAGAAATGTAACTGCTAA+AGG	0.396156	5.3:-2958810	MS.gene22277:CDS
ATTTCTTTGAATTACACTTC+AGG	0.409528	5.3:-2959017	MS.gene22277:CDS
TGACGGTTACATAGAACTTA+AGG	0.410660	5.3:-2958286	MS.gene22277:CDS
ATGGTTTAGGACTGGTGACT+TGG	0.411295	5.3:-2958322	MS.gene22277:CDS
TCCAAAATTAAGACCAAAAG+TGG	0.416587	5.3:+2959149	None:intergenic
GGAAGTGGCTTTCTGATATC+AGG	0.427800	5.3:+2958705	None:intergenic
ATTCTCATAGATCAATGTTC+CGG	0.429813	5.3:+2959100	None:intergenic
GTTGTTGAGGCAGCTGTTGT+TGG	0.430997	5.3:-2958180	MS.gene22277:CDS
TGAAAAGACCAAAAGATGAA+TGG	0.437562	5.3:-2959046	MS.gene22277:CDS
CGAATCATGACGAGTATTCA+AGG	0.441411	5.3:+2959289	None:intergenic
TTCTCATAGATCAATGTTCC+GGG	0.441818	5.3:+2959101	None:intergenic
AAATGCAGCTCATACATTGC+TGG	0.444160	5.3:+2959344	None:intergenic
TCGCCTGCTCCTCTCGAGAC+AGG	0.444470	5.3:+2958529	None:intergenic
CTCAAAATCAAGTTTCCCTT+CGG	0.445425	5.3:+2959070	None:intergenic
GAAGGAGAAGGCAAAGGCTA+TGG	0.446378	5.3:-2957971	MS.gene22277:CDS
GAAAAGACCAAAAGATGAAT+GGG	0.450929	5.3:-2959045	MS.gene22277:CDS
GAGCGCTCCAAGAAGCTGAT+TGG	0.451334	5.3:+2959909	None:intergenic
TAGCCTGTCTCGAGAGGAGC+AGG	0.452101	5.3:-2958532	MS.gene22277:CDS
CCATGTTTCATTGCAATGGA+TGG	0.452299	5.3:-2958878	MS.gene22277:CDS
GTCTCTCCCCAATACTCATC+AGG	0.454603	5.3:+2958153	None:intergenic
TAAGACCAAAAGTGGAAGAT+TGG	0.454652	5.3:+2959157	None:intergenic
CGAAGGATATTATCATATCT+GGG	0.456932	5.3:-2958257	MS.gene22277:CDS
AAAGAAATGTAACTGCTAAA+GGG	0.463695	5.3:-2958809	MS.gene22277:CDS
ATTTGTGTAGGTTGCTGTGT+TGG	0.466354	5.3:-2959378	MS.gene22277:intron
TTGTTGAGGCAGCTGTTGTT+GGG	0.470119	5.3:-2958179	MS.gene22277:CDS
ACAGCTGCCTCAACAACTGC+TGG	0.470476	5.3:+2958186	None:intergenic
CGTCAAGGAGTACAACATAT+TGG	0.470912	5.3:-2958495	MS.gene22277:CDS
GTTCCCATGTTTCATTGCAA+TGG	0.474688	5.3:-2958882	MS.gene22277:CDS
ATTAATGAGTTCAGAACTGT+TGG	0.476604	5.3:+2958738	None:intergenic
AGAAACACCGAGTTGAGAAA+TGG	0.477163	5.3:+2959791	None:intergenic
GATCTGCCAAAGACATCAAC+CGG	0.477410	5.3:-2958015	MS.gene22277:CDS
TTGACAGAAACTTATGGTCC+TGG	0.482770	5.3:-2958585	MS.gene22277:CDS
AAAGGTGGATGGTTTAGGAC+TGG	0.483012	5.3:-2958330	MS.gene22277:CDS
CGGTGTTTCTCCTCATGATG+TGG	0.485617	5.3:-2959778	MS.gene22277:intron
TTGGGAGACCTGATGAGTAT+TGG	0.488351	5.3:-2958161	MS.gene22277:CDS
TTCCATGTGCAAATTGATCC+AGG	0.491293	5.3:+2958567	None:intergenic
CCATCCATTGCAATGAAACA+TGG	0.496766	5.3:+2958878	None:intergenic
GAACGTAGTTAGCGTTGCAT+TGG	0.497591	5.3:+2959940	None:intergenic
TGAATTACTTGATATTGCTA+AGG	0.502727	5.3:-2959210	MS.gene22277:CDS
ACTACTACCTTCCCCGGAAG+TGG	0.505110	5.3:+2958690	None:intergenic
TGGTTTAGGACTGGTGACTT+GGG	0.510160	5.3:-2958321	MS.gene22277:CDS
AAACATTAGCACCATTGAGT+TGG	0.513653	5.3:-2958229	MS.gene22277:CDS
GACTTTGTTATTGTAAACAA+TGG	0.513822	5.3:+2959884	None:intergenic
GCAATGGATGGTGTCTCCCT+TGG	0.517996	5.3:-2958866	MS.gene22277:CDS
TGTGATGAATGGATATCTAA+AGG	0.519167	5.3:-2958379	MS.gene22277:CDS
AGGCGGTGCTCCACCGCCAC+CGG	0.520554	5.3:-2958661	MS.gene22277:CDS
GCTTCTTCCATTTCTCAACT+CGG	0.522221	5.3:-2959798	MS.gene22277:CDS
AATAACACCTTCCAACTCAA+TGG	0.523579	5.3:+2958218	None:intergenic
GGAACAACATCAAGTCCTAA+AGG	0.525359	5.3:-2958996	MS.gene22277:CDS
TTTGCACATGGAAACCAGAA+TGG	0.525741	5.3:-2958557	MS.gene22277:CDS
AGGGGAAACACTGTGATGAA+TGG	0.526822	5.3:-2958390	MS.gene22277:CDS
CATAGAACAGCCCCTGACAT+TGG	0.528095	5.3:+2959314	None:intergenic
CTTTAGAAATGTATTACAGT+CGG	0.538466	5.3:+2957920	None:intergenic
GGTGACATCGTTGTAGATGA+TGG	0.538839	5.3:+2959860	None:intergenic
GCTCATCCATCAGTCTCTCC+CGG	0.539601	5.3:-2959119	MS.gene22277:CDS
CAGAAAGCCACTTCCGGGGA+AGG	0.540398	5.3:-2958697	MS.gene22277:CDS
GTCATCACTACTACCTTCCC+CGG	0.545022	5.3:+2958684	None:intergenic
AAGAGTCATTTAAAGGTGGA+TGG	0.546163	5.3:-2958341	MS.gene22277:CDS
GAATTACTTGATATTGCTAA+GGG	0.549278	5.3:-2959209	MS.gene22277:CDS
AATGTTCCGGGAGAGACTGA+TGG	0.553323	5.3:+2959113	None:intergenic
TATGAGAATCTTATATCCGA+AGG	0.561953	5.3:-2959086	MS.gene22277:CDS
GGTACACTCTTCATTGTGAC+AGG	0.562785	5.3:+2958447	None:intergenic
TTGTGCGTTTGTGAAGTTGA+AGG	0.563096	5.3:-2958127	MS.gene22277:CDS
AAGGAGAAGGCAAAGGCTAT+GGG	0.567730	5.3:-2957970	MS.gene22277:CDS
CCTTGGAGTATTGCTGCTCA+AGG	0.568786	5.3:-2958849	MS.gene22277:CDS
AGTTAGAAGGTGACGATGGA+AGG	0.571786	5.3:-2959969	None:intergenic
CAATGAGATGAAGTCTATGC+CGG	0.575034	5.3:-2958919	MS.gene22277:CDS
CCTAAACATCACCTCACCCA+TGG	0.581531	5.3:+2958410	None:intergenic
GAAGGGAGGGCACAATGCTA+CGG	0.582366	5.3:-2958109	MS.gene22277:CDS
TTTGTTATTGTAAACAATGG+CGG	0.586498	5.3:+2959887	None:intergenic
GGGAAGGTAGTAGTGATGAC+AGG	0.592219	5.3:-2958681	MS.gene22277:CDS
AGGAGTACAACATATTGGAC+TGG	0.595988	5.3:-2958490	MS.gene22277:CDS
TGTGCGTTTGTGAAGTTGAA+GGG	0.597382	5.3:-2958126	MS.gene22277:CDS
AAGAAGCTGATTGGAGTGAG+AGG	0.599442	5.3:+2959918	None:intergenic
GTACAACATATTGGACTGGA+AGG	0.601901	5.3:-2958486	MS.gene22277:CDS
TAGTCATCCAGCAGTTGTTG+AGG	0.610515	5.3:-2958193	MS.gene22277:CDS
AAGCACAAAGTAACACACAC+GGG	0.611637	5.3:-2958768	MS.gene22277:CDS
ATTAGCACCATTGAGTTGGA+AGG	0.615739	5.3:-2958225	MS.gene22277:CDS
CATCCATTGCAATGAAACAT+GGG	0.616932	5.3:+2958879	None:intergenic
AAGGAACAAACCACATCATG+AGG	0.618013	5.3:+2959768	None:intergenic
ATGGAGTAGCCTGTCTCGAG+AGG	0.618262	5.3:-2958538	MS.gene22277:CDS
CCTTGAGCAGCAATACTCCA+AGG	0.618919	5.3:+2958849	None:intergenic
TTCAACTTCACAAACGCACA+AGG	0.624923	5.3:+2958129	None:intergenic
TGGAGTATTGCTGCTCAAGG+TGG	0.625763	5.3:-2958846	MS.gene22277:CDS
GAAGGATATTATCATATCTG+GGG	0.626950	5.3:-2958256	MS.gene22277:CDS
ATGAGAATCTTATATCCGAA+GGG	0.627897	5.3:-2959085	MS.gene22277:CDS
ATGGGTGAGGTGATGTTTAG+GGG	0.630548	5.3:-2958408	MS.gene22277:CDS
TGTCACAATGAAGAGTGTAC+CGG	0.633282	5.3:-2958445	MS.gene22277:CDS
AGAACTTAAGGATCGGTCGA+AGG	0.636389	5.3:-2958274	MS.gene22277:CDS
CGTTTGTGAAGTTGAAGGGA+GGG	0.637801	5.3:-2958122	MS.gene22277:CDS
TTGGGAGTAAAGCATTCTGA+CGG	0.644221	5.3:-2958303	MS.gene22277:CDS
CCGGCTGATGCGAAAACCAT+GGG	0.646339	5.3:-2958426	MS.gene22277:CDS
ACACAAGAGTCATTTAAAGG+TGG	0.651781	5.3:-2958345	MS.gene22277:CDS
GGAACACACCATAAATACAC+CGG	0.652672	5.3:+2958900	None:intergenic
GTCCTGGATCAATTTGCACA+TGG	0.656520	5.3:-2958569	MS.gene22277:CDS
TAAGCACAAAGTAACACACA+CGG	0.660502	5.3:-2958769	MS.gene22277:CDS
CTTGAGCAGCAATACTCCAA+GGG	0.661249	5.3:+2958850	None:intergenic
AGTGGCTTTCTGATATCAGG+TGG	0.661622	5.3:+2958708	None:intergenic
GGTGTAGTATATAGCCATAG+AGG	0.662140	5.3:-2958975	MS.gene22277:CDS
TGATGCGAAAACCATGGGTG+AGG	0.664777	5.3:-2958421	MS.gene22277:CDS
GTTACATAGAACTTAAGGAT+CGG	0.668885	5.3:-2958281	MS.gene22277:CDS
AAGGATATTATCATATCTGG+GGG	0.669368	5.3:-2958255	MS.gene22277:CDS
ATATCAGAAAGCCACTTCCG+GGG	0.673117	5.3:-2958701	MS.gene22277:CDS
ACCGGCTGATGCGAAAACCA+TGG	0.679532	5.3:-2958427	MS.gene22277:CDS
GCGTTTGTGAAGTTGAAGGG+AGG	0.681440	5.3:-2958123	MS.gene22277:CDS
ACAACGATGTCACCTACACA+TGG	0.682235	5.3:-2959851	MS.gene22277:CDS
AGCACAAAGTAACACACACG+GGG	0.696294	5.3:-2958767	MS.gene22277:CDS
GGGAGACCTGATGAGTATTG+GGG	0.710890	5.3:-2958159	MS.gene22277:CDS
AAGGTAGTAGTGATGACAGG+CGG	0.720822	5.3:-2958678	MS.gene22277:CDS
ATGAGTCTGAGACCATGTGT+AGG	0.729803	5.3:+2959839	None:intergenic
CACAAAGTAACACACACGGG+GGG	0.730025	5.3:-2958765	MS.gene22277:CDS
GCACAAAGTAACACACACGG+GGG	0.764468	5.3:-2958766	MS.gene22277:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTCTGTTAATTT+TGG	-	chr5.3:2958219-2958238	MS.gene22277:CDS	10.0%
!!!	AATTTGAATTTGTTTGAAAA+AGG	+	chr5.3:2958141-2958160	None:intergenic	15.0%
!!!	TTTGATTTAGTTCATTCATA+TGG	-	chr5.3:2959278-2959297	MS.gene22277:CDS	20.0%
!	AAAGAAATGTAACTGCTAAA+GGG	-	chr5.3:2959078-2959097	MS.gene22277:CDS	25.0%
!	ATTTCTTTGAATTACACTTC+AGG	-	chr5.3:2958870-2958889	MS.gene22277:CDS	25.0%
!	GAAGAAATATGATTACAAGT+TGG	-	chr5.3:2958439-2958458	MS.gene22277:CDS	25.0%
!	GAAGTGTAATTCAAAGAAAT+TGG	+	chr5.3:2958870-2958889	None:intergenic	25.0%
!	GAATTACTTGATATTGCTAA+GGG	-	chr5.3:2958678-2958697	MS.gene22277:CDS	25.0%
!	GTAATTCATGATCTACAAAA+AGG	+	chr5.3:2958665-2958684	None:intergenic	25.0%
!	TCGCTAGATTATAGATTTAA+AGG	+	chr5.3:2958181-2958200	None:intergenic	25.0%
!	TGAATTACTTGATATTGCTA+AGG	-	chr5.3:2958677-2958696	MS.gene22277:CDS	25.0%
!!	AAACTACTTAGAATTTTGCA+TGG	-	chr5.3:2958383-2958402	MS.gene22277:CDS	25.0%
!!!	AAAATATGTTTTGAAGGAGA+AGG	-	chr5.3:2959904-2959923	MS.gene22277:CDS	25.0%
!!!	GACACAAAAATATGTTTTGA+AGG	-	chr5.3:2959898-2959917	MS.gene22277:CDS	25.0%
!!!	GACTTTGTTATTGTAAACAA+TGG	+	chr5.3:2958006-2958025	None:intergenic	25.0%
!!!	TAATCTAGCGATTTTTCAAT+TGG	-	chr5.3:2958190-2958209	MS.gene22277:CDS	25.0%
!!!	TTAATTTTGGTTTTGATCGA+AGG	-	chr5.3:2958232-2958251	MS.gene22277:CDS	25.0%
!!!	TTTGTTATTGTAAACAATGG+CGG	+	chr5.3:2958003-2958022	None:intergenic	25.0%
	AAGGATATTATCATATCTGG+GGG	-	chr5.3:2959632-2959651	MS.gene22277:intron	30.0%
	AATTAAGATAAGCACCTCTA+TGG	+	chr5.3:2958929-2958948	None:intergenic	30.0%
	AGGTGCTTATCTTAATTCTT+TGG	-	chr5.3:2958932-2958951	MS.gene22277:CDS	30.0%
	ATGAGAATCTTATATCCGAA+GGG	-	chr5.3:2958802-2958821	MS.gene22277:CDS	30.0%
	ATTAATGAGTTCAGAACTGT+TGG	+	chr5.3:2959152-2959171	None:intergenic	30.0%
	ATTCTCATAGATCAATGTTC+CGG	+	chr5.3:2958790-2958809	None:intergenic	30.0%
	CAAAGAAATGTAACTGCTAA+AGG	-	chr5.3:2959077-2959096	MS.gene22277:CDS	30.0%
	CGAAGGATATTATCATATCT+GGG	-	chr5.3:2959630-2959649	MS.gene22277:intron	30.0%
	CTTTAGCAGTTACATTTCTT+TGG	+	chr5.3:2959079-2959098	None:intergenic	30.0%
	GAAAAGACCAAAAGATGAAT+GGG	-	chr5.3:2958842-2958861	MS.gene22277:CDS	30.0%
	GAAGGATATTATCATATCTG+GGG	-	chr5.3:2959631-2959650	MS.gene22277:intron	30.0%
	GAATTTGCACTTATTTGTGT+AGG	-	chr5.3:2958497-2958516	MS.gene22277:CDS	30.0%
	GATATTATAAAGCACAGACT+CGG	+	chr5.3:2958359-2958378	None:intergenic	30.0%
	GTTACATAGAACTTAAGGAT+CGG	-	chr5.3:2959606-2959625	MS.gene22277:intron	30.0%
	TATGAGAATCTTATATCCGA+AGG	-	chr5.3:2958801-2958820	MS.gene22277:CDS	30.0%
	TATGGTTTGACAGAAACTTA+TGG	-	chr5.3:2959296-2959315	MS.gene22277:CDS	30.0%
	TCATACATTGCTGGAATATT+AGG	+	chr5.3:2958537-2958556	None:intergenic	30.0%
	TCCAAAATTAAGACCAAAAG+TGG	+	chr5.3:2958741-2958760	None:intergenic	30.0%
	TCGAAGGATATTATCATATC+TGG	-	chr5.3:2959629-2959648	MS.gene22277:intron	30.0%
	TGAAAAGACCAAAAGATGAA+TGG	-	chr5.3:2958841-2958860	MS.gene22277:CDS	30.0%
	TGTGATGAATGGATATCTAA+AGG	-	chr5.3:2959508-2959527	MS.gene22277:intron	30.0%
!!	AACATATTTTTGTGTCTTGC+CGG	+	chr5.3:2959894-2959913	None:intergenic	30.0%
!!!	ACTTTTGGTCTTAATTTTGG+AGG	-	chr5.3:2958740-2958759	MS.gene22277:CDS	30.0%
!!!	ATTTTTTAGGATGGAAGAAC+TGG	-	chr5.3:2959253-2959272	MS.gene22277:CDS	30.0%
!!!	CTTTTGGTCTTAATTTTGGA+GGG	-	chr5.3:2958741-2958760	MS.gene22277:CDS	30.0%
!!!	TCCACTTTTGGTCTTAATTT+TGG	-	chr5.3:2958737-2958756	MS.gene22277:CDS	30.0%
!!!	TTTTTTAGGATGGAAGAACT+GGG	-	chr5.3:2959254-2959273	MS.gene22277:CDS	30.0%
	AAACATTAGCACCATTGAGT+TGG	-	chr5.3:2959658-2959677	MS.gene22277:intron	35.0%
	AAGAGTCATTTAAAGGTGGA+TGG	-	chr5.3:2959546-2959565	MS.gene22277:intron	35.0%
	AATAACACCTTCCAACTCAA+TGG	+	chr5.3:2959672-2959691	None:intergenic	35.0%
	ACACAAGAGTCATTTAAAGG+TGG	-	chr5.3:2959542-2959561	MS.gene22277:intron	35.0%
	CATCCATTGCAATGAAACAT+GGG	+	chr5.3:2959011-2959030	None:intergenic	35.0%
	CATTTAAAGGTGGATGGTTT+AGG	-	chr5.3:2959552-2959571	MS.gene22277:intron	35.0%
	CTCAAAATCAAGTTTCCCTT+CGG	+	chr5.3:2958820-2958839	None:intergenic	35.0%
	GCAACACAAGAGTCATTTAA+AGG	-	chr5.3:2959539-2959558	MS.gene22277:intron	35.0%
	TAAGACCAAAAGTGGAAGAT+TGG	+	chr5.3:2958733-2958752	None:intergenic	35.0%
	TAAGCACAAAGTAACACACA+CGG	-	chr5.3:2959118-2959137	MS.gene22277:CDS	35.0%
	TCACATATTCACATGTGTCT+AGG	+	chr5.3:2958289-2958308	None:intergenic	35.0%
	TCCATCCTAAAAAATACGTC+CGG	+	chr5.3:2959248-2959267	None:intergenic	35.0%
	TGACGGTTACATAGAACTTA+AGG	-	chr5.3:2959601-2959620	MS.gene22277:intron	35.0%
	TGGCTATATACTACACCTTT+AGG	+	chr5.3:2958909-2958928	None:intergenic	35.0%
	TTCTCATAGATCAATGTTCC+GGG	+	chr5.3:2958789-2958808	None:intergenic	35.0%
!	AACTACCAATCTTCCACTTT+TGG	-	chr5.3:2958725-2958744	MS.gene22277:CDS	35.0%
!	ATTGGATCCCATTCATCTTT+TGG	+	chr5.3:2958852-2958871	None:intergenic	35.0%
!	TACAAAAAGGATTTTCGCGT+CGG	+	chr5.3:2958652-2958671	None:intergenic	35.0%
!!!	ATTTTGGTGTTCCAATGTCA+GGG	-	chr5.3:2958562-2958581	MS.gene22277:CDS	35.0%
	AAATGCAGCTCATACATTGC+TGG	+	chr5.3:2958546-2958565	None:intergenic	40.0%
	AAGCACAAAGTAACACACAC+GGG	-	chr5.3:2959119-2959138	MS.gene22277:CDS	40.0%
	AAGGAACAAACCACATCATG+AGG	+	chr5.3:2958122-2958141	None:intergenic	40.0%
	AGTCTATGCCGGTGTATTTA+TGG	-	chr5.3:2958979-2958998	MS.gene22277:CDS	40.0%
	ATCCTAAAAAATACGTCCGG+TGG	+	chr5.3:2959245-2959264	None:intergenic	40.0%
	ATTTGTGTAGGTTGCTGTGT+TGG	-	chr5.3:2958509-2958528	MS.gene22277:CDS	40.0%
	CAATGAGATGAAGTCTATGC+CGG	-	chr5.3:2958968-2958987	MS.gene22277:CDS	40.0%
	CCATCCATTGCAATGAAACA+TGG	+	chr5.3:2959012-2959031	None:intergenic	40.0%
	CCATGTTTCATTGCAATGGA+TGG	-	chr5.3:2959009-2959028	MS.gene22277:CDS	40.0%
	CGAATCATGACGAGTATTCA+AGG	+	chr5.3:2958601-2958620	None:intergenic	40.0%
	GCTTCTTCCATTTCTCAACT+CGG	-	chr5.3:2958089-2958108	MS.gene22277:CDS	40.0%
	GGAACAACATCAAGTCCTAA+AGG	-	chr5.3:2958891-2958910	MS.gene22277:CDS	40.0%
	GGAACACACCATAAATACAC+CGG	+	chr5.3:2958990-2959009	None:intergenic	40.0%
	GGTGTAGTATATAGCCATAG+AGG	-	chr5.3:2958912-2958931	MS.gene22277:CDS	40.0%
	GTTCCCATGTTTCATTGCAA+TGG	-	chr5.3:2959005-2959024	MS.gene22277:CDS	40.0%
	TACTCGTCATGATTCGTTGA+TGG	-	chr5.3:2958605-2958624	MS.gene22277:CDS	40.0%
	TGATATCAGAAAGCCACTTC+CGG	-	chr5.3:2959184-2959203	MS.gene22277:CDS	40.0%
	TGTGCGTTTGTGAAGTTGAA+GGG	-	chr5.3:2959761-2959780	MS.gene22277:intron	40.0%
	TTCAACTTCACAAACGCACA+AGG	+	chr5.3:2959761-2959780	None:intergenic	40.0%
	TTCCATGTGCAAATTGATCC+AGG	+	chr5.3:2959323-2959342	None:intergenic	40.0%
	TTGACAGAAACTTATGGTCC+TGG	-	chr5.3:2959302-2959321	MS.gene22277:CDS	40.0%
	TTGTGCGTTTGTGAAGTTGA+AGG	-	chr5.3:2959760-2959779	MS.gene22277:intron	40.0%
	TTTGCACATGGAAACCAGAA+TGG	-	chr5.3:2959330-2959349	MS.gene22277:CDS	40.0%
!	AGAAACACCGAGTTGAGAAA+TGG	+	chr5.3:2958099-2958118	None:intergenic	40.0%
!	AGGAGTACAACATATTGGAC+TGG	-	chr5.3:2959397-2959416	MS.gene22277:intron	40.0%
!	CGAAGGGAAACTTGATTTTG+AGG	-	chr5.3:2958818-2958837	MS.gene22277:CDS	40.0%
!	CGTCAAGGAGTACAACATAT+TGG	-	chr5.3:2959392-2959411	MS.gene22277:intron	40.0%
!	GCAATGTATGAGCTGCATTT+TGG	-	chr5.3:2958546-2958565	MS.gene22277:CDS	40.0%
!	GTACAACATATTGGACTGGA+AGG	-	chr5.3:2959401-2959420	MS.gene22277:intron	40.0%
!	TCAGCAAACACGACAGTTTT+TGG	+	chr5.3:2959854-2959873	None:intergenic	40.0%
!	TGTCACAATGAAGAGTGTAC+CGG	-	chr5.3:2959442-2959461	MS.gene22277:intron	40.0%
!	TTGGGAGTAAAGCATTCTGA+CGG	-	chr5.3:2959584-2959603	MS.gene22277:intron	40.0%
!!	AAAGCGTTTGCCTCGTTTTA+TGG	-	chr5.3:2959826-2959845	MS.gene22277:CDS	40.0%
!!	ATTAGCACCATTGAGTTGGA+AGG	-	chr5.3:2959662-2959681	MS.gene22277:intron	40.0%
!!!	ACCGGACGTATTTTTTAGGA+TGG	-	chr5.3:2959244-2959263	MS.gene22277:CDS	40.0%
!!!	CATTTTGGTGTTCCAATGTC+AGG	-	chr5.3:2958561-2958580	MS.gene22277:CDS	40.0%
!!!	GTTTTTGGAGCCATAAAACG+AGG	+	chr5.3:2959839-2959858	None:intergenic	40.0%
!!!	TGTTTTGAAGGAGAAGGCAA+AGG	-	chr5.3:2959910-2959929	MS.gene22277:CDS	40.0%
!!!	TTTTGGTGTTCCAATGTCAG+GGG	-	chr5.3:2958563-2958582	MS.gene22277:CDS	40.0%
	AAGAAGCTGATTGGAGTGAG+AGG	+	chr5.3:2957972-2957991	None:intergenic	45.0%
	AAGGAGAAGGCAAAGGCTAT+GGG	-	chr5.3:2959917-2959936	MS.gene22277:CDS	45.0%
	AAGGTAGTAGTGATGACAGG+CGG	-	chr5.3:2959209-2959228	MS.gene22277:CDS	45.0%
	ACAACGATGTCACCTACACA+TGG	-	chr5.3:2958036-2958055	MS.gene22277:CDS	45.0%
	AGAACTTAAGGATCGGTCGA+AGG	-	chr5.3:2959613-2959632	MS.gene22277:intron	45.0%
	AGCACAAAGTAACACACACG+GGG	-	chr5.3:2959120-2959139	MS.gene22277:CDS	45.0%
	AGGGGAAACACTGTGATGAA+TGG	-	chr5.3:2959497-2959516	MS.gene22277:intron	45.0%
	AGTGGCTTTCTGATATCAGG+TGG	+	chr5.3:2959182-2959201	None:intergenic	45.0%
	ATATCAGAAAGCCACTTCCG+GGG	-	chr5.3:2959186-2959205	MS.gene22277:CDS	45.0%
	ATGGGTGAGGTGATGTTTAG+GGG	-	chr5.3:2959479-2959498	MS.gene22277:intron	45.0%
	CATGGGTGAGGTGATGTTTA+GGG	-	chr5.3:2959478-2959497	MS.gene22277:intron	45.0%
	CGTTTGTGAAGTTGAAGGGA+GGG	-	chr5.3:2959765-2959784	MS.gene22277:intron	45.0%
	CTAAAAAATACGTCCGGTGG+CGG	+	chr5.3:2959242-2959261	None:intergenic	45.0%
	CTTGAGCAGCAATACTCCAA+GGG	+	chr5.3:2959040-2959059	None:intergenic	45.0%
	GAACGTAGTTAGCGTTGCAT+TGG	+	chr5.3:2957950-2957969	None:intergenic	45.0%
	GATATCAGAAAGCCACTTCC+GGG	-	chr5.3:2959185-2959204	MS.gene22277:CDS	45.0%
	GATCTGCCAAAGACATCAAC+CGG	-	chr5.3:2959872-2959891	MS.gene22277:CDS	45.0%
	GCGAAAAAGAAGTCACGTCA+AGG	-	chr5.3:2959377-2959396	MS.gene22277:intron	45.0%
	GGAAGTGGCTTTCTGATATC+AGG	+	chr5.3:2959185-2959204	None:intergenic	45.0%
	GGTACACTCTTCATTGTGAC+AGG	+	chr5.3:2959443-2959462	None:intergenic	45.0%
	GGTGACATCGTTGTAGATGA+TGG	+	chr5.3:2958030-2958049	None:intergenic	45.0%
	GTCCTGGATCAATTTGCACA+TGG	-	chr5.3:2959318-2959337	MS.gene22277:CDS	45.0%
	TAGTCATCCAGCAGTTGTTG+AGG	-	chr5.3:2959694-2959713	MS.gene22277:intron	45.0%
	TCTCACTCCAATCAGCTTCT+TGG	-	chr5.3:2957971-2957990	MS.gene22277:CDS	45.0%
	TGGGAGACCTGATGAGTATT+GGG	-	chr5.3:2959727-2959746	MS.gene22277:intron	45.0%
	TTGGGAGACCTGATGAGTAT+TGG	-	chr5.3:2959726-2959745	MS.gene22277:intron	45.0%
	TTGTTGAGGCAGCTGTTGTT+GGG	-	chr5.3:2959708-2959727	MS.gene22277:intron	45.0%
!	AAAGGTGGATGGTTTAGGAC+TGG	-	chr5.3:2959557-2959576	MS.gene22277:intron	45.0%
!	ATGAGTCTGAGACCATGTGT+AGG	+	chr5.3:2958051-2958070	None:intergenic	45.0%
!	ATGGTTTAGGACTGGTGACT+TGG	-	chr5.3:2959565-2959584	MS.gene22277:intron	45.0%
!	TGGTTTAGGACTGGTGACTT+GGG	-	chr5.3:2959566-2959585	MS.gene22277:intron	45.0%
	AATGTTCCGGGAGAGACTGA+TGG	+	chr5.3:2958777-2958796	None:intergenic	50.0%
	CACAAAGTAACACACACGGG+GGG	-	chr5.3:2959122-2959141	MS.gene22277:CDS	50.0%
	CATAGAACAGCCCCTGACAT+TGG	+	chr5.3:2958576-2958595	None:intergenic	50.0%
	CCATGGGTGAGGTGATGTTT+AGG	-	chr5.3:2959477-2959496	MS.gene22277:intron	50.0%
	CCTAAACATCACCTCACCCA+TGG	+	chr5.3:2959480-2959499	None:intergenic	50.0%
	CCTTGAGCAGCAATACTCCA+AGG	+	chr5.3:2959041-2959060	None:intergenic	50.0%
	CGGTGTTTCTCCTCATGATG+TGG	-	chr5.3:2958109-2958128	MS.gene22277:CDS	50.0%
	GAAGGAGAAGGCAAAGGCTA+TGG	-	chr5.3:2959916-2959935	MS.gene22277:CDS	50.0%
	GCACAAAGTAACACACACGG+GGG	-	chr5.3:2959121-2959140	MS.gene22277:CDS	50.0%
	GCGTTTGTGAAGTTGAAGGG+AGG	-	chr5.3:2959764-2959783	MS.gene22277:intron	50.0%
	GGGAAGGTAGTAGTGATGAC+AGG	-	chr5.3:2959206-2959225	MS.gene22277:CDS	50.0%
	GTATCCGACATGTGTCTGTC+TGG	-	chr5.3:2958263-2958282	MS.gene22277:CDS	50.0%
	GTCATCACTACTACCTTCCC+CGG	+	chr5.3:2959206-2959225	None:intergenic	50.0%
	GTCTCTCCCCAATACTCATC+AGG	+	chr5.3:2959737-2959756	None:intergenic	50.0%
	GTTGTTGAGGCAGCTGTTGT+TGG	-	chr5.3:2959707-2959726	MS.gene22277:intron	50.0%
	TAGGCCAGACAGACACATGT+CGG	+	chr5.3:2958270-2958289	None:intergenic	50.0%
	TGATGCGAAAACCATGGGTG+AGG	-	chr5.3:2959466-2959485	MS.gene22277:intron	50.0%
!	GGGAGACCTGATGAGTATTG+GGG	-	chr5.3:2959728-2959747	MS.gene22277:intron	50.0%
!	GTCTTGCCGGTTGATGTCTT+TGG	+	chr5.3:2959881-2959900	None:intergenic	50.0%
!!	CCTTGGAGTATTGCTGCTCA+AGG	-	chr5.3:2959038-2959057	MS.gene22277:CDS	50.0%
!!	TGGAGTATTGCTGCTCAAGG+TGG	-	chr5.3:2959041-2959060	MS.gene22277:CDS	50.0%
!!!	CGCCACCGGACGTATTTTTT+AGG	-	chr5.3:2959240-2959259	MS.gene22277:CDS	50.0%
	ACAGCTGCCTCAACAACTGC+TGG	+	chr5.3:2959704-2959723	None:intergenic	55.0%
	ACCGGCTGATGCGAAAACCA+TGG	-	chr5.3:2959460-2959479	MS.gene22277:intron	55.0%
	ACTACTACCTTCCCCGGAAG+TGG	+	chr5.3:2959200-2959219	None:intergenic	55.0%
	ATGGAGTAGCCTGTCTCGAG+AGG	-	chr5.3:2959349-2959368	MS.gene22277:CDS	55.0%
	CCGGCTGATGCGAAAACCAT+GGG	-	chr5.3:2959461-2959480	MS.gene22277:intron	55.0%
	CGAGACAGGCTACTCCATTC+TGG	+	chr5.3:2959347-2959366	None:intergenic	55.0%
	GAGCGCTCCAAGAAGCTGAT+TGG	+	chr5.3:2957981-2958000	None:intergenic	55.0%
	GCAATGGATGGTGTCTCCCT+TGG	-	chr5.3:2959021-2959040	MS.gene22277:CDS	55.0%
	GCTCATCCATCAGTCTCTCC+CGG	-	chr5.3:2958768-2958787	MS.gene22277:CDS	55.0%
!	AAAAATACGTCCGGTGGCGG+TGG	+	chr5.3:2959239-2959258	None:intergenic	55.0%
!	CCCATGGTTTTCGCATCAGC+CGG	+	chr5.3:2959464-2959483	None:intergenic	55.0%
!	GAAGGGAGGGCACAATGCTA+CGG	-	chr5.3:2959778-2959797	MS.gene22277:intron	55.0%
	CAGAAAGCCACTTCCGGGGA+AGG	-	chr5.3:2959190-2959209	MS.gene22277:CDS	60.0%
	TAGCCTGTCTCGAGAGGAGC+AGG	-	chr5.3:2959355-2959374	MS.gene22277:CDS	60.0%
	TCGCCTGCTCCTCTCGAGAC+AGG	+	chr5.3:2959361-2959380	None:intergenic	65.0%
	AGGCGGTGCTCCACCGCCAC+CGG	-	chr5.3:2959226-2959245	MS.gene22277:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	2957933	2959976	2957933	ID=MS.gene22277
chr5.3	mRNA	2957933	2959976	2957933	ID=MS.gene22277.t1;Parent=MS.gene22277
chr5.3	exon	2959779	2959976	2959779	ID=MS.gene22277.t1.exon1;Parent=MS.gene22277.t1
chr5.3	CDS	2959779	2959976	2959779	ID=cds.MS.gene22277.t1;Parent=MS.gene22277.t1
chr5.3	exon	2957933	2959390	2957933	ID=MS.gene22277.t1.exon2;Parent=MS.gene22277.t1
chr5.3	CDS	2957933	2959390	2957933	ID=cds.MS.gene22277.t1;Parent=MS.gene22277.t1

Gene Sequence

>MS.gene22277

ATGGAAGGTACTATCCAATGCAACGCTAACTACGTTCCTCTCACTCCAATCAGCTTCTTGGAGCGCTCCGCCATTGTTTACAATAACAAAGTCTCCATCATCTACAACGATGTCACCTACACATGGTCTCAGACTCATCAACGATGTATCAAACTCGCTTCTTCCATTTCTCAACTCGGTGTTTCTCCTCATGATGTGGTTTGTTCCTTTTTCAAACAAATTCAAATTCAACATAGTTTAATTCTCCTTTAAATCTATAATCTAGCGATTTTTCAATTGGATTTGATTTTTTTTTTCTGTTAATTTTGGTTTTGATCGAAGGCGTGTTCAGTATCCGACATGTGTCTGTCTGGCCTAGACACATGTGAATATGTGATGTATCACACTAAATTATGTCATTTTTTAAAATAATTATTGATGTGTCCGAGTCTGTGCTTTATAATATCAATCAAACTACTTAGAATTTTGCATGGAACATGTAAATATGATTATAATTTTTGATTTTAGAAGAAATATGATTACAAGTTGGTTTGAAATTTCTTGTTGATGATTTGTGCTTGTTGTGAATTTGCACTTATTTGTGTAGGTTGCTGTGTTGGCTCCTAATATTCCAGCAATGTATGAGCTGCATTTTGGTGTTCCAATGTCAGGGGCTGTTCTATGTACCTTGAATACTCGTCATGATTCGTTGATGGTTTCGTTGTTGTTAAAGCATTCCGACGCGAAAATCCTTTTTGTAGATCATGAATTACTTGATATTGCTAAGGGAGCACTTGAAATTTTGTCGAAATCAACTACCAATCTTCCACTTTTGGTCTTAATTTTGGAGGGTGATGCTCATCCATCAGTCTCTCCCGGAACATTGATCTATGAGAATCTTATATCCGAAGGGAAACTTGATTTTGAGGTGAAAAGACCAAAAGATGAATGGGATCCAATTTCTTTGAATTACACTTCAGGAACAACATCAAGTCCTAAAGGTGTAGTATATAGCCATAGAGGTGCTTATCTTAATTCTTTGGCTACAATTCTTCTCAATGAGATGAAGTCTATGCCGGTGTATTTATGGTGTGTTCCCATGTTTCATTGCAATGGATGGTGTCTCCCTTGGAGTATTGCTGCTCAAGGTGGCACAAATGTTTGCCAAAGAAATGTAACTGCTAAAGGGATATTTGACAATATTTTTAAGCACAAAGTAACACACACGGGGGGTGCACCAACAGTTCTGAACTCATTAATAAATGCACCACCTGATATCAGAAAGCCACTTCCGGGGAAGGTAGTAGTGATGACAGGCGGTGCTCCACCGCCACCGGACGTATTTTTTAGGATGGAAGAACTGGGATTTGATTTAGTTCATTCATATGGTTTGACAGAAACTTATGGTCCTGGATCAATTTGCACATGGAAACCAGAATGGAGTAGCCTGTCTCGAGAGGAGCAGGCGAAAAAGAAGTCACGTCAAGGAGTACAACATATTGGACTGGAAGGACTTGACATAAAAGATCCTGTCACAATGAAGAGTGTACCGGCTGATGCGAAAACCATGGGTGAGGTGATGTTTAGGGGAAACACTGTGATGAATGGATATCTAAAGGATTTAAAAGCAACACAAGAGTCATTTAAAGGTGGATGGTTTAGGACTGGTGACTTGGGAGTAAAGCATTCTGACGGTTACATAGAACTTAAGGATCGGTCGAAGGATATTATCATATCTGGGGGAGAAAACATTAGCACCATTGAGTTGGAAGGTGTTATTTATAGTCATCCAGCAGTTGTTGAGGCAGCTGTTGTTGGGAGACCTGATGAGTATTGGGGAGAGACACCTTGTGCGTTTGTGAAGTTGAAGGGAGGGCACAATGCTACGGAAGAAGAGATAATACAATTCTGTCAAAAGCGTTTGCCTCGTTTTATGGCTCCAAAAACTGTCGTGTTTGCTGATCTGCCAAAGACATCAACCGGCAAGACACAAAAATATGTTTTGAAGGAGAAGGCAAAGGCTATGGGAAGCTTGTCTAAGAAAAAGAATACAAGCCGACTGTAA

Protein sequence

>MS.gene22277.t1

MEGTIQCNANYVPLTPISFLERSAIVYNNKVSIIYNDVTYTWSQTHQRCIKLASSISQLGVSPHDVVAVLAPNIPAMYELHFGVPMSGAVLCTLNTRHDSLMVSLLLKHSDAKILFVDHELLDIAKGALEILSKSTTNLPLLVLILEGDAHPSVSPGTLIYENLISEGKLDFEVKRPKDEWDPISLNYTSGTTSSPKGVVYSHRGAYLNSLATILLNEMKSMPVYLWCVPMFHCNGWCLPWSIAAQGGTNVCQRNVTAKGIFDNIFKHKVTHTGGAPTVLNSLINAPPDIRKPLPGKVVVMTGGAPPPPDVFFRMEELGFDLVHSYGLTETYGPGSICTWKPEWSSLSREEQAKKKSRQGVQHIGLEGLDIKDPVTMKSVPADAKTMGEVMFRGNTVMNGYLKDLKATQESFKGGWFRTGDLGVKHSDGYIELKDRSKDIIISGGENISTIELEGVIYSHPAVVEAAVVGRPDEYWGETPCAFVKLKGGHNATEEEIIQFCQKRLPRFMAPKTVVFADLPKTSTGKTQKYVLKEKAKAMGSLSKKKNTSRL