AlfalfaGEDB | MS.gene99100

M. sativa cultivar XinJiangDaYe / MS.gene99100

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99100.t1	XP_003616111.1	96.8	562	17	1	1	561	1	562	0.00E+00	1107.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99100.t1	Q9FFE6	64.9	553	187	5	1	551	1	548	3.7e-214	745.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99100.t1	G7K5Y8	96.8	562	17	1	1	561	1	562	0.0e+00	1107.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene88460	MS.gene99100	PPI
MS.gene99100	MS.gene31229	PPI
MS.gene065895	MS.gene99100	PPI
MS.gene026245	MS.gene99100	PPI
MS.gene99100	MS.gene028032	PPI
MS.gene09098	MS.gene99100	PPI
MS.gene99100	MS.gene98148	PPI
MS.gene99100	MS.gene068274	PPI
MS.gene049798	MS.gene99100	PPI
MS.gene99100	MS.gene88987	PPI
MS.gene99100	MS.gene99857	PPI
MS.gene026237	MS.gene99100	PPI
MS.gene99100	MS.gene041843	PPI
MS.gene99100	MS.gene34290	PPI
MS.gene99100	MS.gene061174	PPI
MS.gene43857	MS.gene99100	PPI
MS.gene99100	MS.gene070122	PPI
MS.gene049797	MS.gene99100	PPI
MS.gene72872	MS.gene99100	PPI
MS.gene99100	MS.gene61755	PPI
MS.gene99100	MS.gene23782	PPI
MS.gene99100	MS.gene017279	PPI
MS.gene99100	MS.gene75039	PPI
MS.gene99100	MS.gene24088	PPI
MS.gene99100	MS.gene017547	PPI
MS.gene99100	MS.gene034275	PPI
MS.gene067378	MS.gene99100	PPI
MS.gene99100	MS.gene061578	PPI
MS.gene99100	MS.gene024710	PPI
MS.gene061175	MS.gene99100	PPI
MS.gene007715	MS.gene99100	PPI
MS.gene037817	MS.gene99100	PPI
MS.gene99100	MS.gene88556	PPI
MS.gene049799	MS.gene99100	PPI
MS.gene99100	MS.gene33339	PPI
MS.gene99100	MS.gene83513	PPI
MS.gene72869	MS.gene99100	PPI
MS.gene052937	MS.gene99100	PPI
MS.gene017552	MS.gene99100	PPI
MS.gene99100	MS.gene95914	PPI
MS.gene99100	MS.gene024712	PPI
MS.gene99100	MS.gene79523	PPI
MS.gene028660	MS.gene99100	PPI
MS.gene017545	MS.gene99100	PPI
MS.gene006564	MS.gene99100	PPI
MS.gene99100	MS.gene028035	PPI
MS.gene99100	MS.gene21937	PPI
MS.gene99100	MS.gene21689	PPI
MS.gene99100	MS.gene66371	PPI
MS.gene99100	MS.gene034005	PPI
MS.gene99100	MS.gene05028	PPI
MS.gene99100	MS.gene016573	PPI
MS.gene99100	MS.gene09104	PPI
MS.gene99100	MS.gene23788	PPI
MS.gene037815	MS.gene99100	PPI
MS.gene23786	MS.gene99100	PPI
MS.gene041844	MS.gene99100	PPI
MS.gene99100	MS.gene24887	PPI
MS.gene99100	MS.gene049681	PPI
MS.gene067382	MS.gene99100	PPI
MS.gene070120	MS.gene99100	PPI
MS.gene99100	MS.gene61077	PPI
MS.gene99100	MS.gene008350	PPI
MS.gene99100	MS.gene23786	PPI
MS.gene99100	MS.gene030325	PPI
MS.gene22277	MS.gene99100	PPI
MS.gene96127	MS.gene99100	PPI
MS.gene99100	MS.gene72868	PPI
MS.gene99100	MS.gene96431	PPI
MS.gene99100	MS.gene26216	PPI
MS.gene99100	MS.gene27109	PPI
MS.gene99100	MS.gene09107	PPI
MS.gene99100	MS.gene031746	PPI
MS.gene99100	MS.gene88935	PPI
MS.gene99100	MS.gene27213	PPI
MS.gene99100	MS.gene09153	PPI
MS.gene99100	MS.gene43857	PPI
MS.gene99100	MS.gene067380	PPI
MS.gene000812	MS.gene99100	PPI
MS.gene99100	MS.gene21691	PPI
MS.gene99100	MS.gene037813	PPI
MS.gene33407	MS.gene99100	PPI
MS.gene99100	MS.gene27218	PPI
MS.gene99100	MS.gene028034	PPI
MS.gene025344	MS.gene99100	PPI
MS.gene009614	MS.gene99100	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99100.t1	MTR_5g076240	96.797	562	17	1	1	561	1	562	0.0	1129
MS.gene99100.t1	MTR_5g076220	65.938	549	186	1	1	548	1	549	0.0	771
MS.gene99100.t1	MTR_5g076200	64.610	551	194	1	1	550	1	551	0.0	757
MS.gene99100.t1	MTR_0161s0050	64.247	551	194	2	1	548	1	551	0.0	729
MS.gene99100.t1	MTR_5g008800	53.237	556	244	7	1	552	1	544	0.0	592
MS.gene99100.t1	MTR_8g074040	50.361	554	261	6	1	550	43	586	0.0	582
MS.gene99100.t1	MTR_3g064420	52.753	563	236	8	1	545	1	551	0.0	580
MS.gene99100.t1	MTR_5g008820	50.542	554	260	6	1	552	1	542	0.0	564
MS.gene99100.t1	MTR_4g121600	48.128	561	276	7	1	551	7	562	0.0	546
MS.gene99100.t1	MTR_2g044180	47.482	556	281	6	1	548	7	559	0.0	540
MS.gene99100.t1	MTR_8g074120	50.410	488	228	6	67	550	20	497	4.30e-175	505
MS.gene99100.t1	MTR_8g074040	50.628	478	222	6	77	550	1	468	5.22e-174	501
MS.gene99100.t1	MTR_2g044180	47.046	474	240	6	86	551	1	471	2.95e-153	448
MS.gene99100.t1	MTR_5g023730	42.077	549	298	11	7	548	9	544	8.77e-147	434
MS.gene99100.t1	MTR_0803s0020	30.869	541	332	14	28	553	249	762	1.01e-60	217
MS.gene99100.t1	MTR_2g105570	29.287	519	322	16	39	541	53	542	1.47e-52	188
MS.gene99100.t1	MTR_4g077900	29.703	505	306	14	52	541	1	471	1.67e-47	172
MS.gene99100.t1	MTR_4g128337	30.093	535	315	18	27	542	44	538	1.01e-46	172
MS.gene99100.t1	MTR_1569s0010	28.015	539	343	14	28	541	10	528	2.49e-44	165
MS.gene99100.t1	MTR_4g005750	28.249	531	332	14	27	541	38	535	3.20e-43	162
MS.gene99100.t1	MTR_5g027480	29.389	524	309	19	38	541	66	548	1.33e-42	160
MS.gene99100.t1	MTR_5g027500	27.756	508	324	17	51	541	80	561	2.13e-42	160
MS.gene99100.t1	MTR_3g460770	27.985	536	327	18	41	559	57	550	1.04e-41	158
MS.gene99100.t1	MTR_2g095510	26.508	547	340	16	11	537	24	528	2.38e-41	157
MS.gene99100.t1	MTR_5g007640	26.740	546	320	21	27	542	82	577	1.01e-38	150
MS.gene99100.t1	MTR_3g035130	27.553	519	323	18	40	546	32	509	3.96e-38	147
MS.gene99100.t1	MTR_1g026310	26.195	523	334	14	40	544	68	556	1.14e-37	147
MS.gene99100.t1	MTR_2g030240	26.630	552	358	14	20	542	8	541	1.34e-36	143
MS.gene99100.t1	MTR_8g039720	26.376	527	330	16	40	544	65	555	1.71e-36	143
MS.gene99100.t1	MTR_1g063120	26.770	579	358	15	6	561	16	551	2.63e-36	142
MS.gene99100.t1	MTR_4g057730	25.766	555	353	16	4	537	15	531	4.97e-36	142
MS.gene99100.t1	MTR_1g090737	26.692	517	324	15	36	537	79	555	3.40e-35	139
MS.gene99100.t1	MTR_3g088870	29.396	381	234	11	184	548	197	558	7.31e-34	135
MS.gene99100.t1	MTR_1g063110	26.056	568	338	17	6	537	16	537	1.04e-33	135
MS.gene99100.t1	MTR_3g088880	29.333	375	237	10	185	548	199	556	3.00e-33	134
MS.gene99100.t1	MTR_2g030240	27.830	424	277	11	146	542	26	447	1.45e-31	127
MS.gene99100.t1	MTR_1g073180	25.775	516	325	13	40	543	61	530	2.52e-31	128
MS.gene99100.t1	MTR_7g084850	25.455	550	339	19	37	544	200	720	1.43e-29	124
MS.gene99100.t1	MTR_2g095510	26.432	454	276	15	11	444	24	439	4.21e-29	120
MS.gene99100.t1	MTR_1054s0020	25.693	541	328	19	33	547	279	771	7.71e-29	122
MS.gene99100.t1	MTR_4g057730	25.714	490	309	15	4	472	15	470	1.55e-28	119
MS.gene99100.t1	MTR_3g035130	26.681	461	295	15	40	489	32	460	3.75e-28	118
MS.gene99100.t1	MTR_1586s0010	27.095	358	233	9	51	397	81	421	1.95e-27	115
MS.gene99100.t1	MTR_4g013210	28.535	396	231	15	182	543	64	441	1.62e-26	112
MS.gene99100.t1	MTR_4g011790	27.654	405	241	16	173	543	208	594	1.83e-26	114
MS.gene99100.t1	MTR_4g011790	27.612	402	239	16	173	540	208	591	9.76e-26	112
MS.gene99100.t1	MTR_1g073180	24.395	496	318	12	40	524	83	532	1.91e-25	110
MS.gene99100.t1	MTR_4g011790	26.923	416	239	16	173	543	208	603	5.77e-25	109
MS.gene99100.t1	MTR_4g057730	25.325	462	290	15	4	444	15	442	2.14e-23	103
MS.gene99100.t1	MTR_4g013210	28.042	378	215	15	182	543	64	400	4.65e-22	99.0
MS.gene99100.t1	MTR_4g013210	27.968	379	214	17	182	543	216	552	6.70e-22	99.8
MS.gene99100.t1	MTR_4g013210	28.108	370	214	15	182	517	216	567	1.79e-19	92.4
MS.gene99100.t1	MTR_7g109830	24.074	486	298	14	41	471	78	547	3.64e-17	85.5
MS.gene99100.t1	MTR_1g026470	25.879	313	202	8	40	338	68	364	2.68e-16	81.6
MS.gene99100.t1	MTR_1g026430	25.559	313	203	8	40	338	68	364	1.41e-15	79.3
MS.gene99100.t1	MTR_1g030290	36.000	175	87	9	304	467	424	584	1.68e-13	73.9
MS.gene99100.t1	MTR_3g087980	49.398	83	36	3	391	467	500	582	2.04e-13	73.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99100.t1	AT5G16370	64.919	553	187	5	1	551	1	548	0.0	764
MS.gene99100.t1	AT5G16340	64.610	551	186	6	1	548	1	545	0.0	756
MS.gene99100.t1	AT1G75960	62.000	550	200	6	1	547	1	544	0.0	711
MS.gene99100.t1	AT1G21540	60.870	552	208	6	1	547	1	549	0.0	701
MS.gene99100.t1	AT1G21530	60.469	554	206	9	1	547	1	548	0.0	689
MS.gene99100.t1	AT1G77240	60.073	551	209	6	1	547	1	544	0.0	687
MS.gene99100.t1	AT1G21530	60.469	554	204	10	1	547	1	546	0.0	682
MS.gene99100.t1	AT1G20560	50.000	556	266	6	1	552	3	550	0.0	567
MS.gene99100.t1	AT2G17650	51.964	560	255	7	1	550	48	603	0.0	561
MS.gene99100.t1	AT3G16910	49.201	563	270	7	1	552	9	566	0.0	554
MS.gene99100.t1	AT1G65890	50.000	574	267	6	1	561	1	567	0.0	553
MS.gene99100.t1	AT1G66120	50.265	565	260	8	1	554	1	555	0.0	548
MS.gene99100.t1	AT1G65880	49.378	563	262	7	1	547	1	556	0.0	542
MS.gene99100.t1	AT1G20560	50.208	480	227	6	77	552	1	472	7.57e-168	485
MS.gene99100.t1	AT1G68270	44.624	558	265	8	1	547	1	525	2.72e-156	458
MS.gene99100.t1	AT1G76290	42.602	561	300	8	1	555	1	545	5.00e-156	457
MS.gene99100.t1	AT3G21240	28.820	517	322	14	39	541	63	547	4.27e-49	179
MS.gene99100.t1	AT3G21230	28.462	520	324	16	39	541	73	561	1.36e-43	164
MS.gene99100.t1	AT1G20500	27.655	499	312	14	59	544	84	546	1.11e-41	158
MS.gene99100.t1	AT4G05160	28.154	547	324	17	17	537	30	533	5.96e-41	155
MS.gene99100.t1	AT1G20510	28.107	523	318	17	40	544	58	540	8.62e-41	155
MS.gene99100.t1	AT3G21240	28.348	448	281	12	39	473	63	483	1.91e-38	148
MS.gene99100.t1	AT3G21240	28.348	448	281	12	39	473	63	483	1.91e-38	148
MS.gene99100.t1	AT4G19010	26.789	545	316	19	32	543	63	557	5.88e-38	147
MS.gene99100.t1	AT1G51680	26.703	543	331	15	27	541	51	554	3.25e-37	145
MS.gene99100.t1	AT3G21230	27.699	491	307	16	39	512	73	532	3.81e-37	145
MS.gene99100.t1	AT3G48990	26.208	538	345	16	19	542	9	508	3.95e-37	144
MS.gene99100.t1	AT1G65060	27.289	546	349	15	10	541	46	557	5.57e-37	145
MS.gene99100.t1	AT1G20480	26.582	553	348	17	14	543	43	560	3.59e-36	142
MS.gene99100.t1	AT5G38120	27.668	506	305	17	59	543	85	550	5.92e-35	139
MS.gene99100.t1	AT1G62940	27.799	518	318	17	41	541	55	533	1.07e-34	138
MS.gene99100.t1	AT1G30520	24.653	576	362	14	9	542	2	547	7.38e-34	135
MS.gene99100.t1	AT1G30520	24.653	576	362	14	9	542	2	547	7.38e-34	135
MS.gene99100.t1	AT5G63380	28.841	371	239	9	185	546	202	556	1.50e-33	135
MS.gene99100.t1	AT1G51680	27.049	488	295	13	27	487	51	504	1.92e-32	131
MS.gene99100.t1	AT3G21230	27.434	452	284	15	39	473	73	497	2.89e-31	127
MS.gene99100.t1	AT1G20510	27.273	451	274	16	40	472	58	472	3.18e-30	124
MS.gene99100.t1	AT5G38120	27.021	470	287	16	59	512	85	514	6.54e-29	120
MS.gene99100.t1	AT5G36880	24.863	547	346	17	37	544	195	715	2.36e-28	120
MS.gene99100.t1	AT5G36880	24.909	550	342	18	37	544	145	665	2.58e-28	120
MS.gene99100.t1	AT5G36880	24.909	550	342	18	37	544	145	665	2.58e-28	120
MS.gene99100.t1	AT5G36880	24.909	550	342	18	37	544	145	665	2.58e-28	120
MS.gene99100.t1	AT5G36880	24.909	550	342	18	37	544	145	665	2.58e-28	120
MS.gene99100.t1	AT1G51680	26.371	474	288	13	27	473	51	490	1.16e-27	117
MS.gene99100.t1	AT5G38120	27.021	433	258	16	59	472	85	478	1.35e-25	110
MS.gene99100.t1	AT5G38120	27.021	433	258	16	59	472	85	478	1.35e-25	110
MS.gene99100.t1	AT1G20510	26.351	444	268	16	40	459	58	466	2.20e-24	106
MS.gene99100.t1	AT3G16170	27.390	387	230	15	188	542	235	602	8.86e-24	105
MS.gene99100.t1	AT1G30520	23.542	480	305	11	9	456	2	451	2.78e-21	97.4
MS.gene99100.t1	AT1G30520	23.542	480	305	11	9	456	2	451	2.78e-21	97.4
MS.gene99100.t1	AT1G65060	25.835	449	289	14	10	444	46	464	3.38e-21	97.4
MS.gene99100.t1	AT2G47240	24.540	489	286	17	41	468	19	485	1.70e-18	89.4
MS.gene99100.t1	AT5G36880	23.554	484	300	17	37	478	145	600	4.68e-18	88.2
MS.gene99100.t1	AT1G20490	25.333	375	237	11	59	420	84	428	1.38e-16	82.8
MS.gene99100.t1	AT1G20490	25.333	375	237	11	59	420	84	428	1.38e-16	82.8
MS.gene99100.t1	AT3G21230	25.000	392	249	15	39	414	73	435	4.19e-13	72.0
MS.gene99100.t1	AT1G77590	34.574	188	96	10	303	479	273	444	1.79e-12	70.5
MS.gene99100.t1	AT1G77590	34.574	188	96	10	303	479	419	590	2.39e-12	70.1
MS.gene99100.t1	AT2G04350	32.927	164	93	6	304	460	449	602	3.01e-11	66.6
MS.gene99100.t1	AT2G04350	32.927	164	93	6	304	460	449	602	3.01e-11	66.6

Find 164 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATAAACCCAGTTAAAATT+TGG	0.167962	5.2:+64159374	None:intergenic
AACAGTGTCGTTGTAGATTA+TGG	0.245566	5.2:+64159805	None:intergenic
TTTGGTCTTTGATGAGAATA+AGG	0.251554	5.2:+64159480	None:intergenic
TGGAACCGTCCATTCGATTA+AGG	0.253968	5.2:+64159244	None:intergenic
TTTAGCAACGGTTTATTATC+AGG	0.271697	5.2:+64159023	None:intergenic
CATAGATAAATCGGTTGTTT+TGG	0.282266	5.2:+64159224	None:intergenic
TAGATTATGGAAGGTGTGTT+AGG	0.285753	5.2:+64159818	None:intergenic
TGGCGAGGGAGGATGAGTTT+TGG	0.295270	5.2:-64158485	MS.gene99100:CDS
ATTGGGTCCCACTCTGAGTT+TGG	0.312073	5.2:+64159353	None:intergenic
AGAGGTGGTTGTGTTATGTT+AGG	0.319020	5.2:-64158714	MS.gene99100:CDS
GATATCTACGAAAACGAGTT+TGG	0.319719	5.2:+64159565	None:intergenic
TCCACCGGACAGAAGCTTTA+GGG	0.321988	5.2:-64158953	MS.gene99100:CDS
GAAGAATTTGCCGCATTATA+TGG	0.322752	5.2:-64158373	MS.gene99100:CDS
GTGCCCCAGCGGTTAAAATA+TGG	0.323202	5.2:+64158994	None:intergenic
TTTCGTATGTTTCAAATGTT+TGG	0.332759	5.2:+64159462	None:intergenic
GACAGAAGCTTTAGGGTTTA+CGG	0.333166	5.2:-64158946	MS.gene99100:CDS
CAAAACAACCGATTTATCTA+TGG	0.342133	5.2:-64159223	MS.gene99100:CDS
TTCAACTTACGAAGATCTAA+TGG	0.345402	5.2:-64159408	MS.gene99100:CDS
ATGTTGTTTCGAAGGAAATA+AGG	0.346423	5.2:+64159511	None:intergenic
CTCCACCGGACAGAAGCTTT+AGG	0.361731	5.2:-64158954	MS.gene99100:CDS
GTTGTACATGGAAGAAGAAA+TGG	0.371428	5.2:-64158878	MS.gene99100:CDS
TGGAGGACCGCCGCAGGCGG+CGG	0.373347	5.2:+64158972	None:intergenic
GGTGAATGAGGCGGCGGTGG+TGG	0.380803	5.2:-64158505	MS.gene99100:CDS
TCAAAAGATGTTATTATTAG+TGG	0.384798	5.2:-64158582	MS.gene99100:CDS
CGCTGGGGCACCGCCGCCTG+CGG	0.384853	5.2:-64158982	MS.gene99100:CDS
GAAGAAATGGAATTTGTTAC+CGG	0.393279	5.2:-64158865	MS.gene99100:CDS
GGAGAGTTGTTTGCTTGGGT+TGG	0.395265	5.2:+64159884	None:intergenic
TGGGAATGAACTACACCTTT+TGG	0.397385	5.2:+64159296	None:intergenic
TACGAAGATCTAATGGCTTC+CGG	0.397456	5.2:-64159401	MS.gene99100:CDS
TGAGTGGAGAGTTGTTTGCT+TGG	0.401014	5.2:+64159879	None:intergenic
TTCATACATGGCGGGGATGT+TGG	0.411501	5.2:+64159685	None:intergenic
TCATACATGGCGGGGATGTT+GGG	0.423599	5.2:+64159686	None:intergenic
TGGGGCACCGCCGCCTGCGG+CGG	0.424226	5.2:-64158979	MS.gene99100:CDS
GAGTGGAGAGTTGTTTGCTT+GGG	0.424635	5.2:+64159880	None:intergenic
CGCAGGCGGCGGTGCCCCAG+CGG	0.428869	5.2:+64158983	None:intergenic
GGCGCGTGTTGATGTTGTTA+AGG	0.430348	5.2:+64159630	None:intergenic
TTACGGTAAGTCATGGTTAC+GGG	0.434751	5.2:-64158929	MS.gene99100:CDS
TGAGGCGGCGGTGGTGGCGA+GGG	0.436643	5.2:-64158499	MS.gene99100:CDS
CGCCTGCGGCGGTCCTCCAC+CGG	0.440831	5.2:-64158968	MS.gene99100:CDS
GGCGGTGAATGAGGCGGCGG+TGG	0.443675	5.2:-64158508	MS.gene99100:CDS
AAGAGATATTTGAGTACTGT+AGG	0.450823	5.2:-64158395	MS.gene99100:CDS
GGTAGCGTCCATAGATAAAT+CGG	0.454879	5.2:+64159215	None:intergenic
TGTTGATGTTCTTTCTAAGA+AGG	0.455951	5.2:+64159847	None:intergenic
GACACACATGTGTGGTGCTC+CGG	0.467260	5.2:-64159072	MS.gene99100:CDS
GATTGGTGGTTTGTTGTACA+TGG	0.470182	5.2:-64158890	MS.gene99100:CDS
TGAAAGAAGGGTTGAAGGAA+AGG	0.471669	5.2:-64158434	MS.gene99100:CDS
AAGAAGGTGAGAGGGGTGAG+TGG	0.473343	5.2:+64159863	None:intergenic
ATGAGGCGGCGGTGGTGGCG+AGG	0.474049	5.2:-64158500	MS.gene99100:CDS
AGATTATGGAAGGTGTGTTA+GGG	0.474701	5.2:+64159819	None:intergenic
TCAAATATCTCTTTCTCCGT+TGG	0.476665	5.2:+64158405	None:intergenic
TACCTATGTTCCATGCTAAC+GGG	0.476941	5.2:-64159196	MS.gene99100:CDS
CAAGTTGGAGGCATCGTTGG+TGG	0.479366	5.2:+64159765	None:intergenic
GCCTCCGCCATCACCTCCTT+AGG	0.480944	5.2:-64159743	MS.gene99100:CDS
GAGTTTGAAAGAAGGGTTGA+AGG	0.486977	5.2:-64158439	MS.gene99100:CDS
GTCCGGTGGAGGACCGCCGC+AGG	0.491037	5.2:+64158966	None:intergenic
CCAGTTAAAATTTGGATCAC+CGG	0.496945	5.2:+64159382	None:intergenic
CGGCAAGGAGTGAGAACCCT+AGG	0.499171	5.2:-64158816	MS.gene99100:CDS
GGAACAACGTCGTCACCAAA+AGG	0.503990	5.2:-64159311	MS.gene99100:CDS
GGTATTTAGAGATTAAAGAT+AGG	0.504970	5.2:-64158605	MS.gene99100:CDS
TTCTTTCTAAGAAGGTGAGA+GGG	0.505750	5.2:+64159855	None:intergenic
CTACCTATGTTCCATGCTAA+CGG	0.506459	5.2:-64159197	MS.gene99100:CDS
TTTACGGTAAGTCATGGTTA+CGG	0.507808	5.2:-64158930	MS.gene99100:CDS
GTTTGGATTCGCAGTGGAGG+AGG	0.508080	5.2:+64159582	None:intergenic
ATGTTACTCAACTACACATC+CGG	0.508713	5.2:-64159332	MS.gene99100:CDS
CACTTCGCCGTCCCCTTCAC+CGG	0.514904	5.2:-64159659	MS.gene99100:CDS
GAACTTCCTAAGACTTCTAC+CGG	0.520177	5.2:-64158324	MS.gene99100:CDS
TTAAGGATAGCGCCGGTGAA+GGG	0.521208	5.2:+64159647	None:intergenic
GAGTAGTGTGGAGGTTGAGT+CGG	0.523111	5.2:-64158547	MS.gene99100:CDS
CATCAACCTTTGTCATCCCT+AGG	0.524830	5.2:+64158799	None:intergenic
AGAGGTTTATGTTGTTTCGA+AGG	0.524910	5.2:+64159503	None:intergenic
AAGTGCAGTTCATACATGGC+GGG	0.525594	5.2:+64159677	None:intergenic
GCTAACGGAGACAGGTGGAT+TGG	0.526534	5.2:-64158907	MS.gene99100:CDS
GTTAAGGATAGCGCCGGTGA+AGG	0.526761	5.2:+64159646	None:intergenic
AGGCAAGTTGGAGGCATCGT+TGG	0.528678	5.2:+64159762	None:intergenic
AGGTGATGGCGGAGGCAAGT+TGG	0.530518	5.2:+64159750	None:intergenic
CTAACGGGTGGAGCTTCCCG+TGG	0.532728	5.2:-64159181	MS.gene99100:CDS
ATGTGTAGTTGAGTAACATT+GGG	0.535553	5.2:+64159336	None:intergenic
GGTGAAGTTGTTATGAGAGG+TGG	0.535576	5.2:-64158729	MS.gene99100:CDS
AAACGAGATGGAGCAACGCT+TGG	0.538085	5.2:-64158750	MS.gene99100:CDS
AAGTCATGGTTACGGGCTAA+CGG	0.538797	5.2:-64158922	MS.gene99100:CDS
TAACGGGTGGAGCTTCCCGT+GGG	0.539163	5.2:-64159180	MS.gene99100:CDS
GGCGGCGGTGGTGGCGAGGG+AGG	0.539718	5.2:-64158496	MS.gene99100:CDS
TGGACATCCGGCGGTGAATG+AGG	0.540160	5.2:-64158517	MS.gene99100:CDS
GATGTGTAGTTGAGTAACAT+TGG	0.540709	5.2:+64159335	None:intergenic
GAAAGAAGGGTTGAAGGAAA+GGG	0.542197	5.2:-64158433	MS.gene99100:CDS
CGGTGGAGGACCGCCGCAGG+CGG	0.542338	5.2:+64158969	None:intergenic
CCGTTGATTCCTTAATCGAA+TGG	0.546026	5.2:-64159253	MS.gene99100:CDS
TTCGCAGTGGAGGAGGATGT+TGG	0.547397	5.2:+64159589	None:intergenic
AACCTTGCTCTCTCAGTCGC+CGG	0.548626	5.2:+64158846	None:intergenic
GGTTACGGGCTAACGGAGAC+AGG	0.552343	5.2:-64158915	MS.gene99100:CDS
GGAAGCTCCACCCGTTAGCA+TGG	0.553881	5.2:+64159186	None:intergenic
AACGGAGACAGGTGGATTGG+TGG	0.554790	5.2:-64158904	MS.gene99100:CDS
TCCGGCGGTGAATGAGGCGG+CGG	0.555212	5.2:-64158511	MS.gene99100:CDS
GGCGAAGTGCAGTTCATACA+TGG	0.558435	5.2:+64159673	None:intergenic
TTAGGGTTTACGGTAAGTCA+TGG	0.559864	5.2:-64158936	MS.gene99100:CDS
TGATGGCGGAGGCAAGTTGG+AGG	0.560492	5.2:+64159753	None:intergenic
GATGACAAAGGTTGATGTAG+TGG	0.562684	5.2:-64158793	MS.gene99100:CDS
CTAAAGCTTCTGTCCGGTGG+AGG	0.563322	5.2:+64158955	None:intergenic
GTAGGTGTAATGCATGAAGA+TGG	0.563846	5.2:-64158627	MS.gene99100:CDS
GGGGATGTTGGGAGCGACGA+CGG	0.564910	5.2:+64159697	None:intergenic
GAGGTGGTTGTGTTATGTTA+GGG	0.565042	5.2:-64158713	MS.gene99100:CDS
ACCCTAAAGCTTCTGTCCGG+TGG	0.565348	5.2:+64158952	None:intergenic
GTTGCCGCGGTGGATACCTA+AGG	0.565868	5.2:+64159727	None:intergenic
AAGTTGGAGGCATCGTTGGT+GGG	0.567587	5.2:+64159766	None:intergenic
AAAGATGTTATTATTAGTGG+TGG	0.569732	5.2:-64158579	MS.gene99100:CDS
ATCAACCTTTGTCATCCCTA+GGG	0.569958	5.2:+64158800	None:intergenic
CGAGTTTGGATTCGCAGTGG+AGG	0.571371	5.2:+64159579	None:intergenic
TGATTCCTTAATCGAATGGA+CGG	0.572262	5.2:-64159249	MS.gene99100:CDS
GTGGATACCTAAGGAGGTGA+TGG	0.575209	5.2:+64159736	None:intergenic
ACCTAAGGAGGTGATGGCGG+AGG	0.578934	5.2:+64159742	None:intergenic
ACGGGTGGAGCTTCCCGTGG+GGG	0.582132	5.2:-64159178	MS.gene99100:CDS
TAAACCCTAAAGCTTCTGTC+CGG	0.583489	5.2:+64158949	None:intergenic
ACTACTGCATCCTTCCTAAG+TGG	0.587459	5.2:+64158255	None:intergenic
CTAAGGATATGGGACCACTT+AGG	0.590978	5.2:-64158269	MS.gene99100:CDS
TGGTGAGAATTTGAGTAGTG+TGG	0.591809	5.2:-64158559	MS.gene99100:CDS
GGATATGGGACCACTTAGGA+AGG	0.592975	5.2:-64158265	MS.gene99100:CDS
GATAGCGCCGGTGAAGGGGA+CGG	0.593787	5.2:+64159652	None:intergenic
GTTAACATATTAAGAACCAC+CGG	0.594824	5.2:+64159053	None:intergenic
CGGAGACGACGTTGCCGCGG+TGG	0.595354	5.2:+64159717	None:intergenic
GGTGAAAGTGTAAAACGAGA+TGG	0.596400	5.2:-64158762	MS.gene99100:CDS
TAGGTGTAATGCATGAAGAT+GGG	0.598284	5.2:-64158626	MS.gene99100:CDS
GTTCTTTCTAAGAAGGTGAG+AGG	0.598709	5.2:+64159854	None:intergenic
AGAGAGCAAGGTTGAAGTCA+CGG	0.602793	5.2:-64158836	MS.gene99100:CDS
GATACCTAAGGAGGTGATGG+CGG	0.605808	5.2:+64159739	None:intergenic
TACCGGCGACTGAGAGAGCA+AGG	0.606108	5.2:-64158848	MS.gene99100:CDS
TAAGGATAGCGCCGGTGAAG+GGG	0.606863	5.2:+64159648	None:intergenic
GTGTCGTTGTAGATTATGGA+AGG	0.610708	5.2:+64159809	None:intergenic
AACGGGTGGAGCTTCCCGTG+GGG	0.610896	5.2:-64159179	MS.gene99100:CDS
GTAAGTTGAAATAAAATCAA+CGG	0.612045	5.2:+64159421	None:intergenic
TGGAGATGATACGCGCGTCA+AGG	0.612771	5.2:+64159609	None:intergenic
AGTGCAGTTCATACATGGCG+GGG	0.623126	5.2:+64159678	None:intergenic
GGTTTATCCAAACTCAGAGT+GGG	0.624443	5.2:-64159360	MS.gene99100:CDS
ACCGCCGCCTCATTCACCGC+CGG	0.626771	5.2:+64158510	None:intergenic
CGACGGAGACGACGTTGCCG+CGG	0.628604	5.2:+64159714	None:intergenic
GATGTTGTTAAGGATAGCGC+CGG	0.631854	5.2:+64159640	None:intergenic
CACCCGTTAGCATGGAACAT+AGG	0.633645	5.2:+64159194	None:intergenic
CTATGTTCCATGCTAACGGG+TGG	0.634027	5.2:-64159193	MS.gene99100:CDS
GGGTATTTGAAAGATAGTGA+AGG	0.636873	5.2:-64158693	MS.gene99100:CDS
GCAAGGTTGAAGTCACGGCA+AGG	0.639269	5.2:-64158831	MS.gene99100:CDS
CCATTCGATTAAGGAATCAA+CGG	0.639849	5.2:+64159253	None:intergenic
CGGTTATGATGAAAGTTCCA+CGG	0.640554	5.2:+64159273	None:intergenic
TATGATGAAAGTTCCACGGT+GGG	0.641675	5.2:+64159277	None:intergenic
TTCAAACTCACAAAAGCACA+CGG	0.644342	5.2:+64158453	None:intergenic
ACACATGTGTGGTGCTCCGG+TGG	0.644428	5.2:-64159069	MS.gene99100:CDS
ACATCCGGCGGTGAATGAGG+CGG	0.646521	5.2:-64158514	MS.gene99100:CDS
AAACGAGTTTGGATTCGCAG+TGG	0.650226	5.2:+64159576	None:intergenic
CTTGGTGAAGTTGTTATGAG+AGG	0.659010	5.2:-64158732	MS.gene99100:CDS
GGGTTTATCCAAACTCAGAG+TGG	0.661044	5.2:-64159361	MS.gene99100:CDS
GAAGGAAAGGGAAACACCAA+CGG	0.663516	5.2:-64158421	MS.gene99100:CDS
GGCAAGGAGTGAGAACCCTA+GGG	0.664706	5.2:-64158815	MS.gene99100:CDS
GCCGCGGTGGATACCTAAGG+AGG	0.665926	5.2:+64159730	None:intergenic
TACGGGCTAACGGAGACAGG+TGG	0.672763	5.2:-64158912	MS.gene99100:CDS
AGTTGAAATAAAATCAACGG+TGG	0.674593	5.2:+64159424	None:intergenic
TGAGAATTTGAGTAGTGTGG+AGG	0.682689	5.2:-64158556	MS.gene99100:CDS
TCTTTCTAAGAAGGTGAGAG+GGG	0.682696	5.2:+64159856	None:intergenic
GAAGTGCAGTTCATACATGG+CGG	0.697137	5.2:+64159676	None:intergenic
AACTTCCTAAGACTTCTACC+GGG	0.701789	5.2:-64158323	MS.gene99100:CDS
TCTTTGATGAGAATAAGGAG+AGG	0.708659	5.2:+64159485	None:intergenic
GAGAACCCTAGGGATGACAA+AGG	0.708804	5.2:-64158805	MS.gene99100:CDS
CATCACGTGACACACATGTG+TGG	0.722078	5.2:-64159080	MS.gene99100:CDS
GGTGTAGTTCATTCCCACCG+TGG	0.733199	5.2:-64159290	MS.gene99100:CDS
GTTGATGTAGTGGATCCCAA+TGG	0.754592	5.2:-64158783	MS.gene99100:CDS
TTATGATGAAAGTTCCACGG+TGG	0.773430	5.2:+64159276	None:intergenic
ACCTCCTTAGGTATCCACCG+CGG	0.794220	5.2:-64159731	MS.gene99100:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AACAAAAAATTGTTTTAAGA+AGG	-	chr5.2:64159465-64159484	MS.gene99100:CDS	15.0%
!!!	ACAAAAAATTGTTTTAAGAA+GGG	-	chr5.2:64159466-64159485	MS.gene99100:CDS	15.0%
!!	TCAAAAGATGTTATTATTAG+TGG	-	chr5.2:64159553-64159572	MS.gene99100:CDS	20.0%
!!!	GTAAGTTGAAATAAAATCAA+CGG	+	chr5.2:64158717-64158736	None:intergenic	20.0%
!	AAAGATGTTATTATTAGTGG+TGG	-	chr5.2:64159556-64159575	MS.gene99100:CDS	25.0%
!	AGAAAAATTGCTAAGGATAT+GGG	-	chr5.2:64159856-64159875	MS.gene99100:CDS	25.0%
!	GGTATTTAGAGATTAAAGAT+AGG	-	chr5.2:64159530-64159549	MS.gene99100:CDS	25.0%
!	TAGAAAAATTGCTAAGGATA+TGG	-	chr5.2:64159855-64159874	MS.gene99100:CDS	25.0%
!	TGTACTTAGAAAAATTGCTA+AGG	-	chr5.2:64159849-64159868	MS.gene99100:CDS	25.0%
!	TTTCGTATGTTTCAAATGTT+TGG	+	chr5.2:64158676-64158695	None:intergenic	25.0%
!!!	AAAATTGTTTTAAGAAGGGT+TGG	-	chr5.2:64159470-64159489	MS.gene99100:CDS	25.0%
	AGTTGAAATAAAATCAACGG+TGG	+	chr5.2:64158714-64158733	None:intergenic	30.0%
	ATGTGTAGTTGAGTAACATT+GGG	+	chr5.2:64158802-64158821	None:intergenic	30.0%
	GCATTATATGGTTCCAAAAA+CGG	-	chr5.2:64159774-64159793	MS.gene99100:CDS	30.0%
	GGATAAACCCAGTTAAAATT+TGG	+	chr5.2:64158764-64158783	None:intergenic	30.0%
	GTTAACATATTAAGAACCAC+CGG	+	chr5.2:64159085-64159104	None:intergenic	30.0%
	TTCAACTTACGAAGATCTAA+TGG	-	chr5.2:64158727-64158746	MS.gene99100:CDS	30.0%
	TTTAGCAACGGTTTATTATC+AGG	+	chr5.2:64159115-64159134	None:intergenic	30.0%
!	AAGAGATATTTGAGTACTGT+AGG	-	chr5.2:64159740-64159759	MS.gene99100:CDS	30.0%
!	CAAAACAACCGATTTATCTA+TGG	-	chr5.2:64158912-64158931	MS.gene99100:CDS	30.0%
!	CATAGATAAATCGGTTGTTT+TGG	+	chr5.2:64158914-64158933	None:intergenic	30.0%
!	CTTTTGTGAGTTTGAAAGAA+GGG	-	chr5.2:64159689-64159708	MS.gene99100:CDS	30.0%
!	TACAAACTTCTGAATTTTCC+CGG	+	chr5.2:64159833-64159852	None:intergenic	30.0%
!	TGTTGATGTTCTTTCTAAGA+AGG	+	chr5.2:64158291-64158310	None:intergenic	30.0%
!	TTTGGTCTTTGATGAGAATA+AGG	+	chr5.2:64158658-64158677	None:intergenic	30.0%
!!	ATGTTGTTTCGAAGGAAATA+AGG	+	chr5.2:64158627-64158646	None:intergenic	30.0%
!!	GAAGAAATGGAATTTGTTAC+CGG	-	chr5.2:64159270-64159289	MS.gene99100:CDS	30.0%
!!!	ATATGGACAGTTTTTAGCAA+CGG	+	chr5.2:64159127-64159146	None:intergenic	30.0%
!!!	GTTTTTGGAACCATATAATG+CGG	+	chr5.2:64159775-64159794	None:intergenic	30.0%
	ATGTTACTCAACTACACATC+CGG	-	chr5.2:64158803-64158822	MS.gene99100:CDS	35.0%
	CCAGTTAAAATTTGGATCAC+CGG	+	chr5.2:64158756-64158775	None:intergenic	35.0%
	CCATTCGATTAAGGAATCAA+CGG	+	chr5.2:64158885-64158904	None:intergenic	35.0%
	GAAGAATTTGCCGCATTATA+TGG	-	chr5.2:64159762-64159781	MS.gene99100:CDS	35.0%
	GATATCTACGAAAACGAGTT+TGG	+	chr5.2:64158573-64158592	None:intergenic	35.0%
	GATGTGTAGTTGAGTAACAT+TGG	+	chr5.2:64158803-64158822	None:intergenic	35.0%
	GGGTATTTGAAAGATAGTGA+AGG	-	chr5.2:64159442-64159461	MS.gene99100:CDS	35.0%
	GTTGTACATGGAAGAAGAAA+TGG	-	chr5.2:64159257-64159276	MS.gene99100:CDS	35.0%
	TAGGTGTAATGCATGAAGAT+GGG	-	chr5.2:64159509-64159528	MS.gene99100:CDS	35.0%
	TCAAATATCTCTTTCTCCGT+TGG	+	chr5.2:64159733-64159752	None:intergenic	35.0%
	TCTTTGATGAGAATAAGGAG+AGG	+	chr5.2:64158653-64158672	None:intergenic	35.0%
	TGATTCCTTAATCGAATGGA+CGG	-	chr5.2:64158886-64158905	MS.gene99100:CDS	35.0%
	TTATATGGTTCCAAAAACGG+TGG	-	chr5.2:64159777-64159796	MS.gene99100:CDS	35.0%
	TTTACGGTAAGTCATGGTTA+CGG	-	chr5.2:64159205-64159224	MS.gene99100:CDS	35.0%
!	AACAGTGTCGTTGTAGATTA+TGG	+	chr5.2:64158333-64158352	None:intergenic	35.0%
!	AGATTATGGAAGGTGTGTTA+GGG	+	chr5.2:64158319-64158338	None:intergenic	35.0%
!	CGTTTTACACTTTCACCATT+GGG	+	chr5.2:64159370-64159389	None:intergenic	35.0%
!	GCTTTTGTGAGTTTGAAAGA+AGG	-	chr5.2:64159688-64159707	MS.gene99100:CDS	35.0%
!	TAGATTATGGAAGGTGTGTT+AGG	+	chr5.2:64158320-64158339	None:intergenic	35.0%
!	TCGTTTTACACTTTCACCAT+TGG	+	chr5.2:64159371-64159390	None:intergenic	35.0%
!	TCTTTAAACACCACCGTTTT+TGG	+	chr5.2:64159790-64159809	None:intergenic	35.0%
!	TTCAAACTCACAAAAGCACA+CGG	+	chr5.2:64159685-64159704	None:intergenic	35.0%
!	TTCTTTCTAAGAAGGTGAGA+GGG	+	chr5.2:64158283-64158302	None:intergenic	35.0%
!!	AGAGGTTTATGTTGTTTCGA+AGG	+	chr5.2:64158635-64158654	None:intergenic	35.0%
!!!	CGGTGATCCAAATTTTAACT+GGG	-	chr5.2:64158754-64158773	MS.gene99100:CDS	35.0%
	AACTTCCTAAGACTTCTACC+GGG	-	chr5.2:64159812-64159831	MS.gene99100:CDS	40.0%
	AGAGGTGGTTGTGTTATGTT+AGG	-	chr5.2:64159421-64159440	MS.gene99100:CDS	40.0%
	ATCAACCTTTGTCATCCCTA+GGG	+	chr5.2:64159338-64159357	None:intergenic	40.0%
	CGGTTATGATGAAAGTTCCA+CGG	+	chr5.2:64158865-64158884	None:intergenic	40.0%
	CTACCTATGTTCCATGCTAA+CGG	-	chr5.2:64158938-64158957	MS.gene99100:CDS	40.0%
	CTTGGTGAAGTTGTTATGAG+AGG	-	chr5.2:64159403-64159422	MS.gene99100:CDS	40.0%
	GAAAGAAGGGTTGAAGGAAA+GGG	-	chr5.2:64159702-64159721	MS.gene99100:CDS	40.0%
	GAACTTCCTAAGACTTCTAC+CGG	-	chr5.2:64159811-64159830	MS.gene99100:CDS	40.0%
	GAGGTGGTTGTGTTATGTTA+GGG	-	chr5.2:64159422-64159441	MS.gene99100:CDS	40.0%
	GAGTTTGAAAGAAGGGTTGA+AGG	-	chr5.2:64159696-64159715	MS.gene99100:CDS	40.0%
	GGTAGCGTCCATAGATAAAT+CGG	+	chr5.2:64158923-64158942	None:intergenic	40.0%
	GGTTTATCCAAACTCAGAGT+GGG	-	chr5.2:64158775-64158794	MS.gene99100:CDS	40.0%
	GTAGGTGTAATGCATGAAGA+TGG	-	chr5.2:64159508-64159527	MS.gene99100:CDS	40.0%
	TAAACCCTAAAGCTTCTGTC+CGG	+	chr5.2:64159189-64159208	None:intergenic	40.0%
	TACCTATGTTCCATGCTAAC+GGG	-	chr5.2:64158939-64158958	MS.gene99100:CDS	40.0%
	TACGAAGATCTAATGGCTTC+CGG	-	chr5.2:64158734-64158753	MS.gene99100:CDS	40.0%
	TATGATGAAAGTTCCACGGT+GGG	+	chr5.2:64158861-64158880	None:intergenic	40.0%
	TGAAAGAAGGGTTGAAGGAA+AGG	-	chr5.2:64159701-64159720	MS.gene99100:CDS	40.0%
	TGAGAATTTGAGTAGTGTGG+AGG	-	chr5.2:64159579-64159598	MS.gene99100:CDS	40.0%
	TGGTGAGAATTTGAGTAGTG+TGG	-	chr5.2:64159576-64159595	MS.gene99100:CDS	40.0%
	TTACGGTAAGTCATGGTTAC+GGG	-	chr5.2:64159206-64159225	MS.gene99100:CDS	40.0%
	TTAGGGTTTACGGTAAGTCA+TGG	-	chr5.2:64159199-64159218	MS.gene99100:CDS	40.0%
	TTATGATGAAAGTTCCACGG+TGG	+	chr5.2:64158862-64158881	None:intergenic	40.0%
!	ACAACGACACTGTTTTCACA+TGG	-	chr5.2:64158339-64158358	MS.gene99100:CDS	40.0%
!	ATTTTCCCGGTAGAAGTCTT+AGG	+	chr5.2:64159820-64159839	None:intergenic	40.0%
!	GGTGAAAGTGTAAAACGAGA+TGG	-	chr5.2:64159373-64159392	MS.gene99100:CDS	40.0%
!	GTGTCGTTGTAGATTATGGA+AGG	+	chr5.2:64158329-64158348	None:intergenic	40.0%
!	GTTCTTTCTAAGAAGGTGAG+AGG	+	chr5.2:64158284-64158303	None:intergenic	40.0%
!	TCTTTCTAAGAAGGTGAGAG+GGG	+	chr5.2:64158282-64158301	None:intergenic	40.0%
!	TGGGAATGAACTACACCTTT+TGG	+	chr5.2:64158842-64158861	None:intergenic	40.0%
!!	CCGGTGATCCAAATTTTAAC+TGG	-	chr5.2:64158753-64158772	MS.gene99100:CDS	40.0%
!!	CCGTTGATTCCTTAATCGAA+TGG	-	chr5.2:64158882-64158901	MS.gene99100:CDS	40.0%
!!	GACAGAAGCTTTAGGGTTTA+CGG	-	chr5.2:64159189-64159208	MS.gene99100:CDS	40.0%
!!	GATGACAAAGGTTGATGTAG+TGG	-	chr5.2:64159342-64159361	MS.gene99100:CDS	40.0%
!!	GATTGGTGGTTTGTTGTACA+TGG	-	chr5.2:64159245-64159264	MS.gene99100:CDS	40.0%
!!	TGGTTTTACACTGGTGATGT+AGG	-	chr5.2:64159490-64159509	MS.gene99100:CDS	40.0%
!!!	AAGAAGGGTTGGTTTTACAC+TGG	-	chr5.2:64159481-64159500	MS.gene99100:CDS	40.0%
!!!	ACTGTCCATATTTTAACCGC+TGG	-	chr5.2:64159136-64159155	MS.gene99100:CDS	40.0%
!!!	CTGTCCATATTTTAACCGCT+GGG	-	chr5.2:64159137-64159156	MS.gene99100:CDS	40.0%
!!!	TGTCCATATTTTAACCGCTG+GGG	-	chr5.2:64159138-64159157	MS.gene99100:CDS	40.0%
	AAACGAGTTTGGATTCGCAG+TGG	+	chr5.2:64158562-64158581	None:intergenic	45.0%
	AAGTGCAGTTCATACATGGC+GGG	+	chr5.2:64158461-64158480	None:intergenic	45.0%
	ACTACTGCATCCTTCCTAAG+TGG	+	chr5.2:64159883-64159902	None:intergenic	45.0%
	AGAGAGCAAGGTTGAAGTCA+CGG	-	chr5.2:64159299-64159318	MS.gene99100:CDS	45.0%
	CATCAACCTTTGTCATCCCT+AGG	+	chr5.2:64159339-64159358	None:intergenic	45.0%
	CTAAGGATATGGGACCACTT+AGG	-	chr5.2:64159866-64159885	MS.gene99100:CDS	45.0%
	GAAGGAAAGGGAAACACCAA+CGG	-	chr5.2:64159714-64159733	MS.gene99100:CDS	45.0%
	GAAGTGCAGTTCATACATGG+CGG	+	chr5.2:64158462-64158481	None:intergenic	45.0%
	GAGTGGAGAGTTGTTTGCTT+GGG	+	chr5.2:64158258-64158277	None:intergenic	45.0%
	GATGTTGTTAAGGATAGCGC+CGG	+	chr5.2:64158498-64158517	None:intergenic	45.0%
	GGGTTTATCCAAACTCAGAG+TGG	-	chr5.2:64158774-64158793	MS.gene99100:CDS	45.0%
	GGTGAAGTTGTTATGAGAGG+TGG	-	chr5.2:64159406-64159425	MS.gene99100:CDS	45.0%
	TGAGTGGAGAGTTGTTTGCT+TGG	+	chr5.2:64158259-64158278	None:intergenic	45.0%
	TGGAACCGTCCATTCGATTA+AGG	+	chr5.2:64158894-64158913	None:intergenic	45.0%
!	AAGTCATGGTTACGGGCTAA+CGG	-	chr5.2:64159213-64159232	MS.gene99100:CDS	45.0%
!!	GAGGTTGAGTCGGTTTTGTA+TGG	-	chr5.2:64159598-64159617	MS.gene99100:CDS	45.0%
!!	GTCGGTTTTGTATGGACATC+CGG	-	chr5.2:64159606-64159625	MS.gene99100:CDS	45.0%
!!	GTTGATGTAGTGGATCCCAA+TGG	-	chr5.2:64159352-64159371	MS.gene99100:CDS	45.0%
!!!	TTTTGGTGACGACGTTGTTC+CGG	+	chr5.2:64158825-64158844	None:intergenic	45.0%
	AAACGAGATGGAGCAACGCT+TGG	-	chr5.2:64159385-64159404	MS.gene99100:CDS	50.0%
	AGTGCAGTTCATACATGGCG+GGG	+	chr5.2:64158460-64158479	None:intergenic	50.0%
	ATTGGGTCCCACTCTGAGTT+TGG	+	chr5.2:64158785-64158804	None:intergenic	50.0%
	CACCCGTTAGCATGGAACAT+AGG	+	chr5.2:64158944-64158963	None:intergenic	50.0%
	CATCACGTGACACACATGTG+TGG	-	chr5.2:64159055-64159074	MS.gene99100:CDS	50.0%
	CTATGTTCCATGCTAACGGG+TGG	-	chr5.2:64158942-64158961	MS.gene99100:CDS	50.0%
	GAGAACCCTAGGGATGACAA+AGG	-	chr5.2:64159330-64159349	MS.gene99100:CDS	50.0%
	GAGTAGTGTGGAGGTTGAGT+CGG	-	chr5.2:64159588-64159607	MS.gene99100:CDS	50.0%
	GGAACAACGTCGTCACCAAA+AGG	-	chr5.2:64158824-64158843	MS.gene99100:CDS	50.0%
	GGAGAGTTGTTTGCTTGGGT+TGG	+	chr5.2:64158254-64158273	None:intergenic	50.0%
	GGATATGGGACCACTTAGGA+AGG	-	chr5.2:64159870-64159889	MS.gene99100:CDS	50.0%
	GGCGAAGTGCAGTTCATACA+TGG	+	chr5.2:64158465-64158484	None:intergenic	50.0%
	GTGCCCCAGCGGTTAAAATA+TGG	+	chr5.2:64159144-64159163	None:intergenic	50.0%
	GTGGATACCTAAGGAGGTGA+TGG	+	chr5.2:64158402-64158421	None:intergenic	50.0%
	TCCACCGGACAGAAGCTTTA+GGG	-	chr5.2:64159182-64159201	MS.gene99100:CDS	50.0%
!	GATACCTAAGGAGGTGATGG+CGG	+	chr5.2:64158399-64158418	None:intergenic	50.0%
!	GGCGCGTGTTGATGTTGTTA+AGG	+	chr5.2:64158508-64158527	None:intergenic	50.0%
!	TCATACATGGCGGGGATGTT+GGG	+	chr5.2:64158452-64158471	None:intergenic	50.0%
!	TTCATACATGGCGGGGATGT+TGG	+	chr5.2:64158453-64158472	None:intergenic	50.0%
!!	AAGTTGGAGGCATCGTTGGT+GGG	+	chr5.2:64158372-64158391	None:intergenic	50.0%
!!	GGTTTTGTATGGACATCCGG+CGG	-	chr5.2:64159609-64159628	MS.gene99100:CDS	50.0%
!!	TTAAGGATAGCGCCGGTGAA+GGG	+	chr5.2:64158491-64158510	None:intergenic	50.0%
	AACCTTGCTCTCTCAGTCGC+CGG	+	chr5.2:64159292-64159311	None:intergenic	55.0%
	AAGAAGGTGAGAGGGGTGAG+TGG	+	chr5.2:64158275-64158294	None:intergenic	55.0%
	ACCCTAAAGCTTCTGTCCGG+TGG	+	chr5.2:64159186-64159205	None:intergenic	55.0%
	ACCTCCTTAGGTATCCACCG+CGG	-	chr5.2:64158404-64158423	MS.gene99100:CDS	55.0%
	CGAGTTTGGATTCGCAGTGG+AGG	+	chr5.2:64158559-64158578	None:intergenic	55.0%
	CTAAAGCTTCTGTCCGGTGG+AGG	+	chr5.2:64159183-64159202	None:intergenic	55.0%
	CTCCACCGGACAGAAGCTTT+AGG	-	chr5.2:64159181-64159200	MS.gene99100:CDS	55.0%
	GACACACATGTGTGGTGCTC+CGG	-	chr5.2:64159063-64159082	MS.gene99100:CDS	55.0%
	GCAAGGTTGAAGTCACGGCA+AGG	-	chr5.2:64159304-64159323	MS.gene99100:CDS	55.0%
	GCTAACGGAGACAGGTGGAT+TGG	-	chr5.2:64159228-64159247	MS.gene99100:CDS	55.0%
	GGCAAGGAGTGAGAACCCTA+GGG	-	chr5.2:64159320-64159339	MS.gene99100:CDS	55.0%
	GGTGTAGTTCATTCCCACCG+TGG	-	chr5.2:64158845-64158864	MS.gene99100:CDS	55.0%
	GTTTGGATTCGCAGTGGAGG+AGG	+	chr5.2:64158556-64158575	None:intergenic	55.0%
	TGGAGATGATACGCGCGTCA+AGG	+	chr5.2:64158529-64158548	None:intergenic	55.0%
	TTCGCAGTGGAGGAGGATGT+TGG	+	chr5.2:64158549-64158568	None:intergenic	55.0%
!	GCGAGGGAGGATGAGTTTTG+GGG	-	chr5.2:64159652-64159671	MS.gene99100:CDS	55.0%
!	GGCGAGGGAGGATGAGTTTT+GGG	-	chr5.2:64159651-64159670	MS.gene99100:CDS	55.0%
!	TGGCGAGGGAGGATGAGTTT+TGG	-	chr5.2:64159650-64159669	MS.gene99100:CDS	55.0%
!!	AACGGAGACAGGTGGATTGG+TGG	-	chr5.2:64159231-64159250	MS.gene99100:CDS	55.0%
!!	AGGCAAGTTGGAGGCATCGT+TGG	+	chr5.2:64158376-64158395	None:intergenic	55.0%
!!	CAAGTTGGAGGCATCGTTGG+TGG	+	chr5.2:64158373-64158392	None:intergenic	55.0%
!!	CGAGGGAGGATGAGTTTTGG+GGG	-	chr5.2:64159653-64159672	MS.gene99100:CDS	55.0%
!!	GTTAAGGATAGCGCCGGTGA+AGG	+	chr5.2:64158492-64158511	None:intergenic	55.0%
!!	TAAGGATAGCGCCGGTGAAG+GGG	+	chr5.2:64158490-64158509	None:intergenic	55.0%
!!!	GAGGGAGGATGAGTTTTGGG+GGG	-	chr5.2:64159654-64159673	MS.gene99100:CDS	55.0%
	ACACATGTGTGGTGCTCCGG+TGG	-	chr5.2:64159066-64159085	MS.gene99100:CDS	60.0%
	ACATCCGGCGGTGAATGAGG+CGG	-	chr5.2:64159621-64159640	MS.gene99100:CDS	60.0%
	CAACAGCTGCAATCCCCCAC+GGG	+	chr5.2:64158973-64158992	None:intergenic	60.0%
	CCAACAGCTGCAATCCCCCA+CGG	+	chr5.2:64158974-64158993	None:intergenic	60.0%
	CGGCAAGGAGTGAGAACCCT+AGG	-	chr5.2:64159319-64159338	MS.gene99100:CDS	60.0%
	GGAAGCTCCACCCGTTAGCA+TGG	+	chr5.2:64158952-64158971	None:intergenic	60.0%
	GTTGCCGCGGTGGATACCTA+AGG	+	chr5.2:64158411-64158430	None:intergenic	60.0%
	TACCGGCGACTGAGAGAGCA+AGG	-	chr5.2:64159287-64159306	MS.gene99100:CDS	60.0%
	TGGACATCCGGCGGTGAATG+AGG	-	chr5.2:64159618-64159637	MS.gene99100:CDS	60.0%
	TGGGGGATTGCAGCTGTTGG+TGG	-	chr5.2:64158974-64158993	MS.gene99100:CDS	60.0%
!	GGTTACGGGCTAACGGAGAC+AGG	-	chr5.2:64159220-64159239	MS.gene99100:CDS	60.0%
!	TAACGGGTGGAGCTTCCCGT+GGG	-	chr5.2:64158955-64158974	MS.gene99100:CDS	60.0%
!	TACGGGCTAACGGAGACAGG+TGG	-	chr5.2:64159223-64159242	MS.gene99100:CDS	60.0%
!!	ACCTAAGGAGGTGATGGCGG+AGG	+	chr5.2:64158396-64158415	None:intergenic	60.0%
!!	AGGTGATGGCGGAGGCAAGT+TGG	+	chr5.2:64158388-64158407	None:intergenic	60.0%
!!	TGATGGCGGAGGCAAGTTGG+AGG	+	chr5.2:64158385-64158404	None:intergenic	60.0%
	AACGGGTGGAGCTTCCCGTG+GGG	-	chr5.2:64158956-64158975	MS.gene99100:CDS	65.0%
	CACTTCGCCGTCCCCTTCAC+CGG	-	chr5.2:64158476-64158495	MS.gene99100:CDS	65.0%
	CCGTGGGGGATTGCAGCTGT+TGG	-	chr5.2:64158971-64158990	MS.gene99100:CDS	65.0%
	GCCGCGGTGGATACCTAAGG+AGG	+	chr5.2:64158408-64158427	None:intergenic	65.0%
	GCCTCCGCCATCACCTCCTT+AGG	-	chr5.2:64158392-64158411	MS.gene99100:CDS	65.0%
	GGGGATGTTGGGAGCGACGA+CGG	+	chr5.2:64158441-64158460	None:intergenic	65.0%
!	CTAACGGGTGGAGCTTCCCG+TGG	-	chr5.2:64158954-64158973	MS.gene99100:CDS	65.0%
!!	GATAGCGCCGGTGAAGGGGA+CGG	+	chr5.2:64158486-64158505	None:intergenic	65.0%
	ACCGCCGCCTCATTCACCGC+CGG	+	chr5.2:64159628-64159647	None:intergenic	70.0%
	ACGGGTGGAGCTTCCCGTGG+GGG	-	chr5.2:64158957-64158976	MS.gene99100:CDS	70.0%
	CGACGGAGACGACGTTGCCG+CGG	+	chr5.2:64158424-64158443	None:intergenic	70.0%
	GGTGAATGAGGCGGCGGTGG+TGG	-	chr5.2:64159630-64159649	MS.gene99100:CDS	70.0%
	TCCGGCGGTGAATGAGGCGG+CGG	-	chr5.2:64159624-64159643	MS.gene99100:CDS	70.0%
	CGGAGACGACGTTGCCGCGG+TGG	+	chr5.2:64158421-64158440	None:intergenic	75.0%
	GGCGGTGAATGAGGCGGCGG+TGG	-	chr5.2:64159627-64159646	MS.gene99100:CDS	75.0%
!	ATGAGGCGGCGGTGGTGGCG+AGG	-	chr5.2:64159635-64159654	MS.gene99100:CDS	75.0%
!	TGAGGCGGCGGTGGTGGCGA+GGG	-	chr5.2:64159636-64159655	MS.gene99100:CDS	75.0%
!	CGCCTGCGGCGGTCCTCCAC+CGG	-	chr5.2:64159167-64159186	MS.gene99100:CDS	80.0%
!	CGGTGGAGGACCGCCGCAGG+CGG	+	chr5.2:64159169-64159188	None:intergenic	80.0%
!	GTCCGGTGGAGGACCGCCGC+AGG	+	chr5.2:64159172-64159191	None:intergenic	80.0%
!	TGGAGGACCGCCGCAGGCGG+CGG	+	chr5.2:64159166-64159185	None:intergenic	80.0%
!	CGCAGGCGGCGGTGCCCCAG+CGG	+	chr5.2:64159155-64159174	None:intergenic	85.0%
!!	GGCGGCGGTGGTGGCGAGGG+AGG	-	chr5.2:64159639-64159658	MS.gene99100:CDS	85.0%
!!!	CGCTGGGGCACCGCCGCCTG+CGG	-	chr5.2:64159153-64159172	MS.gene99100:CDS	85.0%
!!!	TGGGGCACCGCCGCCTGCGG+CGG	-	chr5.2:64159156-64159175	MS.gene99100:CDS	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	64158236	64159921	64158236	ID=MS.gene99100
chr5.2	mRNA	64158236	64159921	64158236	ID=MS.gene99100.t1;Parent=MS.gene99100
chr5.2	exon	64158236	64159921	64158236	ID=MS.gene99100.t1.exon1;Parent=MS.gene99100.t1
chr5.2	CDS	64158236	64159921	64158236	ID=cds.MS.gene99100.t1;Parent=MS.gene99100.t1

Gene Sequence

>MS.gene99100

ATGGAACAACTAAAACCAACCCAAGCAAACAACTCTCCACTCACCCCTCTCACCTTCTTAGAAAGAACATCAACAATCTACCCTAACACACCTTCCATAATCTACAACGACACTGTTTTCACATGGTCTCAAACCCACCAACGATGCCTCCAACTTGCCTCCGCCATCACCTCCTTAGGTATCCACCGCGGCAACGTCGTCTCCGTCGTCGCTCCCAACATCCCCGCCATGTATGAACTGCACTTCGCCGTCCCCTTCACCGGCGCTATCCTTAACAACATCAACACGCGCCTTGACGCGCGTATCATCTCCAACATCCTCCTCCACTGCGAATCCAAACTCGTTTTCGTAGATATCGCTGCACGTGATCTTGTTCTTCAAGCTTTATCCTTATTTCCTTCGAAACAACATAAACCTCTCCTTATTCTCATCAAAGACCAAACATTTGAAACATACGAAAATGTTTCTTCATCTTCCACCGTTGATTTTATTTCAACTTACGAAGATCTAATGGCTTCCGGTGATCCAAATTTTAACTGGGTTTATCCAAACTCAGAGTGGGACCCAATGTTACTCAACTACACATCCGGAACAACGTCGTCACCAAAAGGTGTAGTTCATTCCCACCGTGGAACTTTCATCATAACCGTTGATTCCTTAATCGAATGGACGGTTCCAAAACAACCGATTTATCTATGGACGCTACCTATGTTCCATGCTAACGGGTGGAGCTTCCCGTGGGGGATTGCAGCTGTTGGTGGAACAAACATCTGTGTAAGAAAATTCGACGCTGAAATTGTTTTTTCTCTCATCAGAAAACATCACGTGACACACATGTGTGGTGCTCCGGTGGTTCTTAATATGTTAACTAACTCACCTGATAATAAACCGTTGCTAAAAACTGTCCATATTTTAACCGCTGGGGCACCGCCGCCTGCGGCGGTCCTCCACCGGACAGAAGCTTTAGGGTTTACGGTAAGTCATGGTTACGGGCTAACGGAGACAGGTGGATTGGTGGTTTGTTGTACATGGAAGAAGAAATGGAATTTGTTACCGGCGACTGAGAGAGCAAGGTTGAAGTCACGGCAAGGAGTGAGAACCCTAGGGATGACAAAGGTTGATGTAGTGGATCCCAATGGTGAAAGTGTAAAACGAGATGGAGCAACGCTTGGTGAAGTTGTTATGAGAGGTGGTTGTGTTATGTTAGGGTATTTGAAAGATAGTGAAGGAACAAAAAATTGTTTTAAGAAGGGTTGGTTTTACACTGGTGATGTAGGTGTAATGCATGAAGATGGGTATTTAGAGATTAAAGATAGGTCAAAAGATGTTATTATTAGTGGTGGTGAGAATTTGAGTAGTGTGGAGGTTGAGTCGGTTTTGTATGGACATCCGGCGGTGAATGAGGCGGCGGTGGTGGCGAGGGAGGATGAGTTTTGGGGGGAGACACCGTGTGCTTTTGTGAGTTTGAAAGAAGGGTTGAAGGAAAGGGAAACACCAACGGAGAAAGAGATATTTGAGTACTGTAGGAAGAATTTGCCGCATTATATGGTTCCAAAAACGGTGGTGTTTAAAGATGAACTTCCTAAGACTTCTACCGGGAAAATTCAGAAGTTTGTACTTAGAAAAATTGCTAAGGATATGGGACCACTTAGGAAGGATGCAGTAGTCACAAAAAGTCGTATGTGA

Protein sequence

>MS.gene99100.t1

MEQLKPTQANNSPLTPLTFLERTSTIYPNTPSIIYNDTVFTWSQTHQRCLQLASAITSLGIHRGNVVSVVAPNIPAMYELHFAVPFTGAILNNINTRLDARIISNILLHCESKLVFVDIAARDLVLQALSLFPSKQHKPLLILIKDQTFETYENVSSSSTVDFISTYEDLMASGDPNFNWVYPNSEWDPMLLNYTSGTTSSPKGVVHSHRGTFIITVDSLIEWTVPKQPIYLWTLPMFHANGWSFPWGIAAVGGTNICVRKFDAEIVFSLIRKHHVTHMCGAPVVLNMLTNSPDNKPLLKTVHILTAGAPPPAAVLHRTEALGFTVSHGYGLTETGGLVVCCTWKKKWNLLPATERARLKSRQGVRTLGMTKVDVVDPNGESVKRDGATLGEVVMRGGCVMLGYLKDSEGTKNCFKKGWFYTGDVGVMHEDGYLEIKDRSKDVIISGGENLSSVEVESVLYGHPAVNEAAVVAREDEFWGETPCAFVSLKEGLKERETPTEKEIFEYCRKNLPHYMVPKTVVFKDELPKTSTGKIQKFVLRKIAKDMGPLRKDAVVTKSRM