AlfalfaGEDB | MS.gene26216

M. sativa cultivar XinJiangDaYe / MS.gene26216

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26216.t1	XP_003616111.1	96.6	562	14	2	1	557	1	562	0.00E+00	1100.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26216.t1	Q9FFE6	65.0	552	185	5	1	548	1	548	3.3e-215	749.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26216.t1	G7K5Y8	96.6	562	14	2	1	557	1	562	0.0e+00	1100.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene061174	MS.gene26216	PPI
MS.gene23786	MS.gene26216	PPI
MS.gene041844	MS.gene26216	PPI
MS.gene037817	MS.gene26216	PPI
MS.gene067382	MS.gene26216	PPI
MS.gene070120	MS.gene26216	PPI
MS.gene24088	MS.gene26216	PPI
MS.gene21937	MS.gene26216	PPI
MS.gene017552	MS.gene26216	PPI
MS.gene22277	MS.gene26216	PPI
MS.gene028660	MS.gene26216	PPI
MS.gene26216	MS.gene024712	PPI
MS.gene27213	MS.gene26216	PPI
MS.gene017547	MS.gene26216	PPI
MS.gene23782	MS.gene26216	PPI
MS.gene017545	MS.gene26216	PPI
MS.gene028032	MS.gene26216	PPI
MS.gene034005	MS.gene26216	PPI
MS.gene26216	MS.gene66371	PPI
MS.gene037815	MS.gene26216	PPI
MS.gene34290	MS.gene26216	PPI
MS.gene95914	MS.gene26216	PPI
MS.gene21689	MS.gene26216	PPI
MS.gene75039	MS.gene26216	PPI
MS.gene05028	MS.gene26216	PPI
MS.gene24887	MS.gene26216	PPI
MS.gene26216	MS.gene049681	PPI
MS.gene61077	MS.gene26216	PPI
MS.gene016573	MS.gene26216	PPI
MS.gene009614	MS.gene26216	PPI
MS.gene09107	MS.gene26216	PPI
MS.gene028035	MS.gene26216	PPI
MS.gene065895	MS.gene26216	PPI
MS.gene26216	MS.gene030325	PPI
MS.gene061578	MS.gene26216	PPI
MS.gene96127	MS.gene26216	PPI
MS.gene72872	MS.gene26216	PPI
MS.gene026245	MS.gene26216	PPI
MS.gene09098	MS.gene26216	PPI
MS.gene26216	MS.gene72868	PPI
MS.gene068274	MS.gene26216	PPI
MS.gene99857	MS.gene26216	PPI
MS.gene26216	MS.gene27109	PPI
MS.gene26216	MS.gene88935	PPI
MS.gene21691	MS.gene26216	PPI
MS.gene026237	MS.gene26216	PPI
MS.gene26216	MS.gene88987	PPI
MS.gene31229	MS.gene26216	PPI
MS.gene96431	MS.gene26216	PPI
MS.gene26216	MS.gene067380	PPI
MS.gene000812	MS.gene26216	PPI
MS.gene034275	MS.gene26216	PPI
MS.gene33407	MS.gene26216	PPI
MS.gene024710	MS.gene26216	PPI
MS.gene049797	MS.gene26216	PPI
MS.gene025344	MS.gene26216	PPI
MS.gene26216	MS.gene028034	PPI
MS.gene88460	MS.gene26216	PPI
MS.gene031746	MS.gene26216	PPI
MS.gene070122	MS.gene26216	PPI
MS.gene049798	MS.gene26216	PPI
MS.gene26216	MS.gene98148	PPI
MS.gene061175	MS.gene26216	PPI
MS.gene007715	MS.gene26216	PPI
MS.gene88556	MS.gene26216	PPI
MS.gene27218	MS.gene26216	PPI
MS.gene33339	MS.gene26216	PPI
MS.gene43857	MS.gene26216	PPI
MS.gene049799	MS.gene26216	PPI
MS.gene09104	MS.gene26216	PPI
MS.gene26216	MS.gene061174	PPI
MS.gene99100	MS.gene26216	PPI
MS.gene72869	MS.gene26216	PPI
MS.gene79523	MS.gene26216	PPI
MS.gene037813	MS.gene26216	PPI
MS.gene052937	MS.gene26216	PPI
MS.gene83513	MS.gene26216	PPI
MS.gene008350	MS.gene26216	PPI
MS.gene26216	MS.gene61755	PPI
MS.gene017279	MS.gene26216	PPI
MS.gene26216	MS.gene017279	PPI
MS.gene006564	MS.gene26216	PPI
MS.gene23788	MS.gene26216	PPI
MS.gene041843	MS.gene26216	PPI
MS.gene09153	MS.gene26216	PPI
MS.gene067378	MS.gene26216	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26216.t1	MTR_5g076240	96.619	562	14	2	1	557	1	562	0.0	1121
MS.gene26216.t1	MTR_5g076220	65.938	549	183	2	1	545	1	549	0.0	770
MS.gene26216.t1	MTR_5g076200	64.685	555	192	2	1	551	1	555	0.0	758
MS.gene26216.t1	MTR_0161s0050	64.324	555	184	3	1	545	1	551	0.0	732
MS.gene26216.t1	MTR_5g008800	53.526	553	244	6	1	549	1	544	0.0	593
MS.gene26216.t1	MTR_8g074040	50.722	554	256	7	1	547	43	586	0.0	583
MS.gene26216.t1	MTR_3g064420	52.973	555	244	7	1	542	1	551	0.0	580
MS.gene26216.t1	MTR_5g008820	50.817	551	260	6	1	549	1	542	0.0	567
MS.gene26216.t1	MTR_4g121600	48.214	560	274	7	1	548	7	562	0.0	544
MS.gene26216.t1	MTR_2g044180	47.935	557	274	7	1	545	7	559	0.0	542
MS.gene26216.t1	MTR_8g074120	51.025	488	222	8	67	547	20	497	4.22e-177	510
MS.gene26216.t1	MTR_8g074040	51.046	478	217	7	77	547	1	468	5.17e-174	501
MS.gene26216.t1	MTR_2g044180	47.579	475	233	7	86	548	1	471	1.53e-154	451
MS.gene26216.t1	MTR_5g023730	42.336	548	295	11	7	545	9	544	3.77e-148	437
MS.gene26216.t1	MTR_0803s0020	31.836	512	319	12	28	533	249	736	1.03e-61	220
MS.gene26216.t1	MTR_2g105570	29.537	518	319	17	39	538	53	542	8.10e-52	186
MS.gene26216.t1	MTR_4g077900	30.080	502	305	15	52	538	1	471	6.97e-46	168
MS.gene26216.t1	MTR_4g128337	29.511	532	319	19	27	539	44	538	9.11e-45	166
MS.gene26216.t1	MTR_1569s0010	28.281	541	336	15	28	538	10	528	1.51e-44	166
MS.gene26216.t1	MTR_4g005750	28.273	527	334	15	27	538	38	535	9.11e-43	160
MS.gene26216.t1	MTR_5g027480	29.367	521	310	19	38	538	66	548	1.26e-42	160
MS.gene26216.t1	MTR_5g027500	27.866	506	323	17	51	538	80	561	4.76e-42	159
MS.gene26216.t1	MTR_2g095510	27.273	550	329	18	11	534	24	528	7.37e-42	158
MS.gene26216.t1	MTR_3g460770	28.101	516	317	17	41	539	57	535	2.43e-41	157
MS.gene26216.t1	MTR_5g007640	27.307	531	333	20	27	539	82	577	1.63e-38	149
MS.gene26216.t1	MTR_3g035130	27.821	514	325	18	40	543	32	509	2.37e-38	148
MS.gene26216.t1	MTR_1g026310	26.346	520	334	14	40	541	68	556	8.64e-38	147
MS.gene26216.t1	MTR_8g039720	26.226	530	324	17	40	541	65	555	2.56e-36	142
MS.gene26216.t1	MTR_1g090737	26.834	518	319	16	36	534	79	555	6.71e-36	142
MS.gene26216.t1	MTR_2g030240	26.594	549	359	14	20	539	8	541	2.96e-35	139
MS.gene26216.t1	MTR_4g057730	26.297	559	342	20	4	534	15	531	3.99e-35	139
MS.gene26216.t1	MTR_1g063110	25.704	568	337	17	6	534	16	537	6.54e-35	139
MS.gene26216.t1	MTR_1g063120	26.773	564	360	16	6	544	16	551	7.42e-35	138
MS.gene26216.t1	MTR_3g088870	29.659	381	233	12	181	545	197	558	2.71e-33	134
MS.gene26216.t1	MTR_3g088880	29.600	375	236	11	182	545	199	556	6.23e-33	133
MS.gene26216.t1	MTR_1g073180	25.391	512	329	11	40	540	61	530	2.69e-31	128
MS.gene26216.t1	MTR_2g030240	28.198	383	250	9	181	539	66	447	8.84e-31	125
MS.gene26216.t1	MTR_7g084850	24.954	549	340	18	37	541	200	720	6.89e-30	125
MS.gene26216.t1	MTR_2g095510	27.033	455	269	16	11	441	24	439	8.61e-30	122
MS.gene26216.t1	MTR_1586s0010	26.420	352	240	6	51	394	81	421	1.30e-29	121
MS.gene26216.t1	MTR_1054s0020	25.698	537	330	17	33	544	279	771	5.87e-29	122
MS.gene26216.t1	MTR_3g035130	26.535	456	299	13	40	486	32	460	1.75e-28	119
MS.gene26216.t1	MTR_4g057730	26.113	494	299	18	4	469	15	470	9.38e-28	116
MS.gene26216.t1	MTR_4g011790	28.395	405	238	17	170	540	208	594	2.68e-27	116
MS.gene26216.t1	MTR_4g011790	28.358	402	236	17	170	537	208	591	1.86e-26	114
MS.gene26216.t1	MTR_4g013210	28.788	396	230	16	179	540	64	441	3.87e-26	111
MS.gene26216.t1	MTR_4g011790	27.644	416	236	17	170	540	208	603	1.13e-25	111
MS.gene26216.t1	MTR_1g073180	23.984	492	322	10	40	521	83	532	1.76e-25	110
MS.gene26216.t1	MTR_4g057730	25.966	466	279	18	4	441	15	442	1.25e-22	101
MS.gene26216.t1	MTR_4g013210	28.042	378	215	16	179	540	64	400	5.39e-22	99.0
MS.gene26216.t1	MTR_4g013210	28.232	379	213	18	179	540	216	552	1.65e-21	98.6
MS.gene26216.t1	MTR_4g013210	28.378	370	213	16	179	514	216	567	4.55e-19	91.3
MS.gene26216.t1	MTR_7g109830	23.760	484	299	13	41	468	78	547	1.18e-17	87.0
MS.gene26216.t1	MTR_1g026470	26.129	310	202	8	40	335	68	364	1.21e-16	82.8
MS.gene26216.t1	MTR_1g026430	25.806	310	203	8	40	335	68	364	7.63e-16	80.1
MS.gene26216.t1	MTR_1g030290	34.066	182	94	9	295	464	417	584	1.80e-13	73.6
MS.gene26216.t1	MTR_3g087980	49.398	83	36	3	388	464	500	582	2.14e-13	73.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26216.t1	AT5G16370	65.036	552	185	5	1	548	1	548	0.0	766
MS.gene26216.t1	AT5G16340	64.481	549	187	5	1	545	1	545	0.0	758
MS.gene26216.t1	AT1G75960	62.295	549	197	6	1	544	1	544	0.0	714
MS.gene26216.t1	AT1G21540	60.838	549	210	4	1	544	1	549	0.0	706
MS.gene26216.t1	AT1G21530	60.799	551	206	9	1	544	1	548	0.0	694
MS.gene26216.t1	AT1G77240	60.584	548	208	5	1	544	1	544	0.0	694
MS.gene26216.t1	AT1G21530	60.799	551	204	10	1	544	1	546	0.0	687
MS.gene26216.t1	AT1G20560	50.000	558	270	5	1	554	3	555	0.0	574
MS.gene26216.t1	AT2G17650	51.786	560	253	7	1	547	48	603	0.0	563
MS.gene26216.t1	AT3G16910	49.464	560	270	7	1	549	9	566	0.0	553
MS.gene26216.t1	AT1G65890	50.626	559	257	6	1	544	1	555	0.0	552
MS.gene26216.t1	AT1G66120	50.633	553	259	6	1	544	1	548	0.0	552
MS.gene26216.t1	AT1G65880	49.643	560	262	6	1	544	1	556	0.0	548
MS.gene26216.t1	AT1G20560	50.207	482	231	5	77	554	1	477	8.17e-171	493
MS.gene26216.t1	AT1G68270	45.242	557	260	9	1	544	1	525	7.26e-157	459
MS.gene26216.t1	AT1G76290	43.376	551	293	8	1	545	1	538	3.15e-156	458
MS.gene26216.t1	AT3G21240	29.045	513	323	14	39	538	63	547	7.94e-50	181
MS.gene26216.t1	AT3G21230	28.627	517	324	15	39	538	73	561	2.49e-45	168
MS.gene26216.t1	AT1G20500	28.169	497	309	14	59	541	84	546	4.95e-43	162
MS.gene26216.t1	AT4G05160	28.309	544	324	17	17	534	30	533	1.04e-40	155
MS.gene26216.t1	AT1G20510	27.885	520	320	17	40	541	58	540	7.29e-40	152
MS.gene26216.t1	AT3G21240	28.090	445	283	12	39	470	63	483	5.27e-39	149
MS.gene26216.t1	AT3G21240	28.090	445	283	12	39	470	63	483	5.27e-39	149
MS.gene26216.t1	AT3G21230	27.869	488	307	15	39	509	73	532	9.89e-39	149
MS.gene26216.t1	AT1G65060	28.125	544	344	17	10	538	46	557	6.85e-38	147
MS.gene26216.t1	AT4G19010	27.087	539	319	19	32	540	63	557	1.00e-37	147
MS.gene26216.t1	AT3G48990	26.679	536	342	16	19	539	9	508	5.49e-37	144
MS.gene26216.t1	AT1G20480	27.027	555	340	19	14	540	43	560	6.69e-37	144
MS.gene26216.t1	AT1G30520	24.782	573	362	14	9	539	2	547	2.19e-36	143
MS.gene26216.t1	AT1G30520	24.782	573	362	14	9	539	2	547	2.19e-36	143
MS.gene26216.t1	AT1G51680	27.462	528	343	15	27	538	51	554	2.56e-36	143
MS.gene26216.t1	AT1G62940	28.350	515	316	18	41	538	55	533	2.09e-35	140
MS.gene26216.t1	AT5G38120	27.579	504	305	18	59	540	85	550	2.92e-35	139
MS.gene26216.t1	AT3G21230	27.617	449	284	14	39	470	73	497	6.05e-33	132
MS.gene26216.t1	AT5G63380	29.380	371	237	9	182	543	202	556	8.25e-33	132
MS.gene26216.t1	AT1G51680	27.778	486	315	14	27	495	51	517	7.69e-32	129
MS.gene26216.t1	AT1G20510	27.009	448	276	16	40	469	58	472	8.56e-30	122
MS.gene26216.t1	AT5G38120	26.923	468	287	17	59	509	85	514	2.44e-29	122
MS.gene26216.t1	AT5G36880	25.000	548	341	18	37	541	145	665	6.22e-29	122
MS.gene26216.t1	AT5G36880	25.000	548	341	18	37	541	145	665	6.22e-29	122
MS.gene26216.t1	AT5G36880	25.000	548	341	18	37	541	145	665	6.22e-29	122
MS.gene26216.t1	AT5G36880	25.000	548	341	18	37	541	145	665	6.22e-29	122
MS.gene26216.t1	AT5G36880	24.954	545	345	17	37	541	195	715	6.67e-29	122
MS.gene26216.t1	AT1G51680	26.638	458	304	13	27	470	51	490	7.23e-27	114
MS.gene26216.t1	AT5G38120	27.103	428	261	15	59	469	85	478	6.74e-26	111
MS.gene26216.t1	AT5G38120	27.103	428	261	15	59	469	85	478	6.74e-26	111
MS.gene26216.t1	AT3G16170	27.649	387	229	15	185	539	235	602	1.79e-24	108
MS.gene26216.t1	AT1G20510	26.077	441	270	16	40	456	58	466	5.81e-24	105
MS.gene26216.t1	AT1G30520	23.480	477	306	11	9	453	2	451	7.58e-23	102
MS.gene26216.t1	AT1G30520	23.480	477	306	11	9	453	2	451	7.58e-23	102
MS.gene26216.t1	AT1G65060	26.398	447	286	15	10	441	46	464	1.84e-21	98.2
MS.gene26216.t1	AT2G47240	25.102	490	279	18	41	465	78	544	9.04e-20	93.6
MS.gene26216.t1	AT2G47240	25.102	490	279	18	41	465	78	544	9.04e-20	93.6
MS.gene26216.t1	AT2G47240	25.102	490	279	18	41	465	78	544	9.04e-20	93.6
MS.gene26216.t1	AT2G47240	25.102	490	279	18	41	465	19	485	1.18e-19	93.2
MS.gene26216.t1	AT5G36880	23.859	482	298	17	37	475	145	600	4.97e-19	91.3
MS.gene26216.t1	AT1G20490	25.806	372	236	11	59	417	84	428	1.19e-17	86.3
MS.gene26216.t1	AT1G20490	25.806	372	236	11	59	417	84	428	1.19e-17	86.3
MS.gene26216.t1	AT3G21230	25.193	389	249	14	39	411	73	435	1.01e-14	77.0
MS.gene26216.t1	AT1G77590	34.574	188	96	10	300	476	273	444	1.80e-12	70.5
MS.gene26216.t1	AT1G77590	34.574	188	96	10	300	476	419	590	2.22e-12	70.5
MS.gene26216.t1	AT4G14070	27.004	237	141	8	283	503	448	668	1.54e-11	67.8
MS.gene26216.t1	AT2G04350	32.927	164	93	6	301	457	449	602	4.09e-11	66.2
MS.gene26216.t1	AT2G04350	32.927	164	93	6	301	457	449	602	4.09e-11	66.2

Find 159 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATAAACCCAGTCAAAATT+TGG	0.177147	5.4:-58195198	None:intergenic
AGGTAGCGTCCATAGATAAA+TGG	0.234318	5.4:-58195358	None:intergenic
TTTGGTCTTTGATGAGAATA+AGG	0.251554	5.4:-58195101	None:intergenic
AAGAGATATTTGAGTATTGT+AGG	0.253055	5.4:+58196177	MS.gene26216:CDS
TGGCAAGGGAGGATGAGTTT+TGG	0.268166	5.4:+58196087	MS.gene26216:CDS
CATAGATAAATGGGTTGTTT+TGG	0.282266	5.4:-58195348	None:intergenic
ATTGGGTCCCACTCTGAGTT+TGG	0.312073	5.4:-58195219	None:intergenic
CTCCACCGTACTGAAGCTTT+AGG	0.313320	5.4:+58195618	MS.gene26216:CDS
AGAGGTGGTTGTGTTATGTT+AGG	0.319020	5.4:+58195858	MS.gene26216:CDS
GAAGAATTTGCCGCATTATA+TGG	0.322752	5.4:+58196199	MS.gene26216:CDS
CAAAACAACCCATTTATCTA+TGG	0.336379	5.4:+58195349	MS.gene26216:CDS
GTGCTCCAGCTGTTAAAATA+TGG	0.337925	5.4:-58195578	None:intergenic
TTCAACTTACGAAGATCTAA+TGG	0.345402	5.4:+58195164	MS.gene26216:CDS
TCCACCGTACTGAAGCTTTA+GGG	0.348758	5.4:+58195619	MS.gene26216:CDS
GATGTCAACGAAAACGAGTT+TGG	0.351198	5.4:-58195016	None:intergenic
AGATTGTTGATGTTCTTTCT+AGG	0.354091	5.4:-58194738	None:intergenic
GTTGTACATGGAAGAAGAAA+TGG	0.371428	5.4:+58195694	MS.gene26216:CDS
GGTGAATGAGGCGGCGGTGG+TGG	0.380803	5.4:+58196067	MS.gene26216:CDS
TCAAAAGATGTTATTATTAG+TGG	0.384798	5.4:+58195990	MS.gene26216:CDS
TTCATACATGGCGGGGATGT+TGG	0.389163	5.4:-58194896	None:intergenic
GGAGAGTTGTTTGCTTGGGT+TGG	0.395265	5.4:-58194697	None:intergenic
TGGGAATGAACTACACCTTT+TGG	0.397385	5.4:-58195276	None:intergenic
TACGAAGATCTAATGGCTTC+CGG	0.397456	5.4:+58195171	MS.gene26216:CDS
TGAGTGGAGAGTTGTTTGCT+TGG	0.401014	5.4:-58194702	None:intergenic
TGGAGAACCGCCGCAGGCGG+CGG	0.418559	5.4:-58195600	None:intergenic
ATGTTACTCAACTACACTTC+CGG	0.423263	5.4:+58195240	MS.gene26216:CDS
TCATACATGGCGGGGATGTT+GGG	0.423599	5.4:-58194895	None:intergenic
GAGTGGAGAGTTGTTTGCTT+GGG	0.424635	5.4:-58194701	None:intergenic
GGCGCGTGTTGATGTTGTTA+AGG	0.430348	5.4:-58194951	None:intergenic
TTACAGTGAGTCATGGTTAC+GGG	0.438402	5.4:+58195643	MS.gene26216:CDS
TGAGGCGGCGGTGGTGGCAA+GGG	0.439213	5.4:+58196073	MS.gene26216:CDS
GGCGGTGAATGAGGCGGCGG+TGG	0.443675	5.4:+58196064	MS.gene26216:CDS
TGGAGCACCGCCGCCTGCGG+CGG	0.446124	5.4:+58195593	MS.gene26216:CDS
TGAAAGAAGGGTTGAAGGAA+AGG	0.463316	5.4:+58196138	MS.gene26216:CDS
ATGAGGCGGCGGTGGTGGCA+AGG	0.465014	5.4:+58196072	MS.gene26216:CDS
AGCTGGAGCACCGCCGCCTG+CGG	0.470098	5.4:+58195590	MS.gene26216:CDS
GATTGGTGGTTTGTTGTACA+TGG	0.470182	5.4:+58195682	MS.gene26216:CDS
AGGAAGGTGAGAGGGGTGAG+TGG	0.473343	5.4:-58194718	None:intergenic
TCAAATATCTCTTTCTCCGT+TGG	0.475709	5.4:-58196167	None:intergenic
GGTAGCGTCCATAGATAAAT+GGG	0.476183	5.4:-58195357	None:intergenic
AACCTTGCTCTCTCCGTCGC+TGG	0.476442	5.4:-58195726	None:intergenic
TACCTATGTTCCATGCTAAC+GGG	0.476941	5.4:+58195376	MS.gene26216:CDS
GACACACATGTGCGGTGCTC+CGG	0.478003	5.4:+58195500	MS.gene26216:CDS
AAGGGAATAAGGATAAAGCT+TGG	0.478093	5.4:-58195059	None:intergenic
CAAGTTGGAGGCATCGTTGG+TGG	0.479366	5.4:-58194816	None:intergenic
TAGATGATGGAAGGTGTGTT+GGG	0.483900	5.4:-58194763	None:intergenic
GAGTTTGAAAGAAGGGTTGA+AGG	0.486977	5.4:+58196133	MS.gene26216:CDS
CGGCAAGGAGTGAGAACCCT+AGG	0.499171	5.4:+58195756	MS.gene26216:CDS
GGAACAACGTCGTCACCAAA+AGG	0.503990	5.4:+58195261	MS.gene26216:CDS
GGTATTTAGAGATTAAAGAT+AGG	0.504970	5.4:+58195967	MS.gene26216:CDS
CTACCTATGTTCCATGCTAA+CGG	0.506459	5.4:+58195375	MS.gene26216:CDS
TTTACAGTGAGTCATGGTTA+CGG	0.507417	5.4:+58195642	MS.gene26216:CDS
AACGGTGTCGTTGTAGATGA+TGG	0.514741	5.4:-58194776	None:intergenic
TTCTTTCTAGGAAGGTGAGA+GGG	0.516818	5.4:-58194726	None:intergenic
GCCTCCGCTATCACCTCCTT+AGG	0.517641	5.4:+58194838	MS.gene26216:CDS
GAACTTCCTAAGACTTCTAC+CGG	0.520177	5.4:+58196248	MS.gene26216:CDS
TTAAGGATAGCGCCGGTGAA+GGG	0.521208	5.4:-58194934	None:intergenic
CACTTCGCCGTCCCCTTCAC+CGG	0.522016	5.4:+58194922	MS.gene26216:CDS
TGTTGATGTTCTTTCTAGGA+AGG	0.523006	5.4:-58194734	None:intergenic
GAGTAGTGTGGAGGTTGAGT+CGG	0.523111	5.4:+58196025	MS.gene26216:CDS
GAAGTGTAGTTGAGTAACAT+TGG	0.523302	5.4:-58195237	None:intergenic
GTTTGGATTCACAGTGGAGG+AGG	0.524317	5.4:-58194999	None:intergenic
CATCAACCTTTGTCATCCCT+AGG	0.524830	5.4:-58195773	None:intergenic
AGGTGATAGCGGAGGCAAGT+TGG	0.525074	5.4:-58194831	None:intergenic
AAGTGGAGTTCATACATGGC+GGG	0.525594	5.4:-58194904	None:intergenic
GCTAACGGAGACAGGTGGAT+TGG	0.526534	5.4:+58195665	MS.gene26216:CDS
GTTAAGGATAGCGCCGGTGA+AGG	0.526761	5.4:-58194935	None:intergenic
AGGCAAGTTGGAGGCATCGT+TGG	0.528678	5.4:-58194819	None:intergenic
CCAGTCAAAATTTGGATCAC+CGG	0.529442	5.4:-58195190	None:intergenic
GTGACTGGATGCTTCCTAAG+TGG	0.529827	5.4:-58196317	None:intergenic
GGCGGCGGTGGTGGCAAGGG+AGG	0.530904	5.4:+58196076	MS.gene26216:CDS
CTAACGGGTGGAGCTTCCCG+TGG	0.532728	5.4:+58195391	MS.gene26216:CDS
GGTGAAGTTGTTATGAGAGG+TGG	0.535576	5.4:+58195843	MS.gene26216:CDS
AAGTGTAGTTGAGTAACATT+GGG	0.537573	5.4:-58195236	None:intergenic
AAACGAGATGGAGCAACGCT+TGG	0.538085	5.4:+58195822	MS.gene26216:CDS
TAACGGGTGGAGCTTCCCGT+GGG	0.539163	5.4:+58195392	MS.gene26216:CDS
TGGACATCCGGCGGTGAATG+AGG	0.540160	5.4:+58196055	MS.gene26216:CDS
CCGTTGATTCATTAATCGAA+TGG	0.540568	5.4:+58195319	MS.gene26216:CDS
GTTGCCACGGCGGATACCTA+AGG	0.542689	5.4:-58194854	None:intergenic
GAAAGAAGGGTTGAAGGAAA+GGG	0.546935	5.4:+58196139	MS.gene26216:CDS
GGTTACGGGCTAACGGAGAC+AGG	0.552343	5.4:+58195657	MS.gene26216:CDS
GGAAGCTCCACCCGTTAGCA+TGG	0.553881	5.4:-58195386	None:intergenic
AACGGAGACAGGTGGATTGG+TGG	0.554790	5.4:+58195668	MS.gene26216:CDS
TCCGGCGGTGAATGAGGCGG+CGG	0.555212	5.4:+58196061	MS.gene26216:CDS
GATGACAAAGGTTGATGTAG+TGG	0.562684	5.4:+58195779	MS.gene26216:CDS
GTAGGTGTAATGCATGAAGA+TGG	0.563846	5.4:+58195945	MS.gene26216:CDS
GAGGTGGTTGTGTTATGTTA+GGG	0.565042	5.4:+58195859	MS.gene26216:CDS
GGATTCACAGTGGAGGAGGA+TGG	0.566219	5.4:-58194995	None:intergenic
GTAGATGATGGAAGGTGTGT+TGG	0.566868	5.4:-58194764	None:intergenic
AAGTTGGAGGCATCGTTGGT+GGG	0.567587	5.4:-58194815	None:intergenic
AAAGATGTTATTATTAGTGG+TGG	0.569732	5.4:+58195993	MS.gene26216:CDS
ATCAACCTTTGTCATCCCTA+GGG	0.569958	5.4:-58195772	None:intergenic
GGGGATGTTGGGAGCAACGA+CGG	0.570572	5.4:-58194884	None:intergenic
TTAGGGTTTACAGTGAGTCA+TGG	0.572267	5.4:+58195636	MS.gene26216:CDS
ATGGAATTTGTTACCAGCGA+CGG	0.572780	5.4:+58195713	MS.gene26216:CDS
TGATTCATTAATCGAATGGA+CGG	0.574282	5.4:+58195323	MS.gene26216:CDS
CGACGGAGACGACGTTGCCA+CGG	0.574292	5.4:-58194867	None:intergenic
ACACATGTGCGGTGCTCCGG+TGG	0.575801	5.4:+58195503	MS.gene26216:CDS
GGCGAAGTGGAGTTCATACA+TGG	0.576977	5.4:-58194908	None:intergenic
GAGTCATGGTTACGGGCTAA+CGG	0.578018	5.4:+58195650	MS.gene26216:CDS
ACGGGTGGAGCTTCCCGTGG+GGG	0.582132	5.4:+58195394	MS.gene26216:CDS
TACCAGCGACGGAGAGAGCA+AGG	0.583476	5.4:+58195724	MS.gene26216:CDS
GGTTTGAGACCATGTGAAAA+CGG	0.588780	5.4:-58194794	None:intergenic
CTAAGGACATGGGACCACTT+AGG	0.591204	5.4:+58196303	MS.gene26216:CDS
TGGTGAGAATTTGAGTAGTG+TGG	0.591809	5.4:+58196013	MS.gene26216:CDS
GATAGCGCCGGTGAAGGGGA+CGG	0.593787	5.4:-58194929	None:intergenic
GTTAACATATTAAGAACCAC+CGG	0.594824	5.4:-58195519	None:intergenic
CGGTGGAGAACCGCCGCAGG+CGG	0.595171	5.4:-58195603	None:intergenic
GGTGAAAGTGTAAAACGAGA+TGG	0.596400	5.4:+58195810	MS.gene26216:CDS
TGATAGCGGAGGCAAGTTGG+AGG	0.597902	5.4:-58194828	None:intergenic
TAGGTGTAATGCATGAAGAT+GGG	0.598284	5.4:+58195946	MS.gene26216:CDS
GTACGGTGGAGAACCGCCGC+AGG	0.600346	5.4:-58195606	None:intergenic
AGAGAGCAAGGTTGAAGTCA+CGG	0.602793	5.4:+58195736	MS.gene26216:CDS
TGGAGATGATACGCGCGTCA+AGG	0.606851	5.4:-58194972	None:intergenic
TAAGGATAGCGCCGGTGAAG+GGG	0.606863	5.4:-58194933	None:intergenic
AACGGGTGGAGCTTCCCGTG+GGG	0.610896	5.4:+58195393	MS.gene26216:CDS
CGGTGAAGGGGACGGCGAAG+TGG	0.613496	5.4:-58194921	None:intergenic
GTAAGTTGAAATAAAATCAA+CGG	0.615697	5.4:-58195151	None:intergenic
CCATTCGATTAATGAATCAA+CGG	0.615830	5.4:-58195319	None:intergenic
AGTGGAGTTCATACATGGCG+GGG	0.616611	5.4:-58194903	None:intergenic
TTCACAGTGGAGGAGGATGG+TGG	0.617991	5.4:-58194992	None:intergenic
GTTCTTTCTAGGAAGGTGAG+AGG	0.622922	5.4:-58194727	None:intergenic
GGTTTATCCAAACTCAGAGT+GGG	0.624443	5.4:+58195212	MS.gene26216:CDS
ACCGCCGCCTCATTCACCGC+CGG	0.626771	5.4:-58196062	None:intergenic
GATGTTGTTAAGGATAGCGC+CGG	0.631854	5.4:-58194941	None:intergenic
CACCCGTTAGCATGGAACAT+AGG	0.633645	5.4:-58195378	None:intergenic
CTATGTTCCATGCTAACGGG+TGG	0.634027	5.4:+58195379	MS.gene26216:CDS
GGGTATTTGAAAGATAGTGA+AGG	0.636873	5.4:+58195879	MS.gene26216:CDS
GCAAGGTTGAAGTCACGGCA+AGG	0.639269	5.4:+58195741	MS.gene26216:CDS
CGGTTATGATGAAAGTTCCA+CGG	0.640554	5.4:-58195299	None:intergenic
TATGATGAAAGTTCCACGGT+GGG	0.641675	5.4:-58195295	None:intergenic
TTCAAACTCACAAAAGCACA+CGG	0.644342	5.4:-58196119	None:intergenic
ACATCCGGCGGTGAATGAGG+CGG	0.646521	5.4:+58196058	MS.gene26216:CDS
TCTTTCTAGGAAGGTGAGAG+GGG	0.647068	5.4:-58194725	None:intergenic
ACCCTAAAGCTTCAGTACGG+TGG	0.647704	5.4:-58195620	None:intergenic
CGAGTTTGGATTCACAGTGG+AGG	0.647951	5.4:-58195002	None:intergenic
TAAACCCTAAAGCTTCAGTA+CGG	0.654658	5.4:-58195623	None:intergenic
CTTGGTGAAGTTGTTATGAG+AGG	0.659010	5.4:+58195840	MS.gene26216:CDS
GGGTTTATCCAAACTCAGAG+TGG	0.661044	5.4:+58195211	MS.gene26216:CDS
ACCTAAGGAGGTGATAGCGG+AGG	0.663636	5.4:-58194839	None:intergenic
GGCAAGGAGTGAGAACCCTA+GGG	0.664706	5.4:+58195757	MS.gene26216:CDS
GATACCTAAGGAGGTGATAG+CGG	0.665932	5.4:-58194842	None:intergenic
GAAGGAAAGGGATATACCAA+CGG	0.665980	5.4:+58196151	MS.gene26216:CDS
GCCACGGCGGATACCTAAGG+AGG	0.671985	5.4:-58194851	None:intergenic
TACGGGCTAACGGAGACAGG+TGG	0.672763	5.4:+58195660	MS.gene26216:CDS
GTGTCGTTGTAGATGATGGA+AGG	0.678506	5.4:-58194772	None:intergenic
TGAGAATTTGAGTAGTGTGG+AGG	0.682689	5.4:+58196016	MS.gene26216:CDS
CATCACGTGACACACATGTG+CGG	0.688035	5.4:+58195492	MS.gene26216:CDS
GAAGTGGAGTTCATACATGG+CGG	0.691480	5.4:-58194905	None:intergenic
TTGGAGAACAAGATCACGTG+CGG	0.694992	5.4:-58195040	None:intergenic
AACTTCCTAAGACTTCTACC+GGG	0.701789	5.4:+58196249	MS.gene26216:CDS
CGGAGACGACGTTGCCACGG+CGG	0.706270	5.4:-58194864	None:intergenic
TCTTTGATGAGAATAAGGAG+AGG	0.708659	5.4:-58195096	None:intergenic
GAGAACCCTAGGGATGACAA+AGG	0.708804	5.4:+58195767	MS.gene26216:CDS
AAACGAGTTTGGATTCACAG+TGG	0.732789	5.4:-58195005	None:intergenic
GGTGTAGTTCATTCCCACCG+TGG	0.733199	5.4:+58195282	MS.gene26216:CDS
ACCTCCTTAGGTATCCGCCG+TGG	0.736799	5.4:+58194850	MS.gene26216:CDS
GTTGATGTAGTGGATCCCAA+TGG	0.754592	5.4:+58195789	MS.gene26216:CDS
TTATGATGAAAGTTCCACGG+TGG	0.773430	5.4:-58195296	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AACAAAAAATTGTTTTAAGA+AGG	+	chr5.4:58195902-58195921	MS.gene26216:CDS	15.0%
!!!	ACAAAAAATTGTTTTAAGAA+GGG	+	chr5.4:58195903-58195922	MS.gene26216:CDS	15.0%
!!	TCAAAAGATGTTATTATTAG+TGG	+	chr5.4:58195990-58196009	MS.gene26216:CDS	20.0%
!!!	AAGAAACATTTTCAAATGTT+TGG	-	chr5.4:58195122-58195141	None:intergenic	20.0%
!!!	GTAAGTTGAAATAAAATCAA+CGG	-	chr5.4:58195154-58195173	None:intergenic	20.0%
!	AAAGATGTTATTATTAGTGG+TGG	+	chr5.4:58195993-58196012	MS.gene26216:CDS	25.0%
!	GGTATTTAGAGATTAAAGAT+AGG	+	chr5.4:58195967-58195986	MS.gene26216:CDS	25.0%
!	TGTACTTAGAAAAATTGCTA+AGG	+	chr5.4:58196286-58196305	MS.gene26216:CDS	25.0%
!!	AAGAGATATTTGAGTATTGT+AGG	+	chr5.4:58196177-58196196	MS.gene26216:CDS	25.0%
!!!	AAAATTGTTTTAAGAAGGGT+TGG	+	chr5.4:58195907-58195926	MS.gene26216:CDS	25.0%
	AAGTGTAGTTGAGTAACATT+GGG	-	chr5.4:58195239-58195258	None:intergenic	30.0%
	AGAAAAATTGCTAAGGACAT+GGG	+	chr5.4:58196293-58196312	MS.gene26216:CDS	30.0%
	AGATTGTTGATGTTCTTTCT+AGG	-	chr5.4:58194741-58194760	None:intergenic	30.0%
	CAAAACAACCCATTTATCTA+TGG	+	chr5.4:58195349-58195368	MS.gene26216:CDS	30.0%
	CCATTCGATTAATGAATCAA+CGG	-	chr5.4:58195322-58195341	None:intergenic	30.0%
	GCATTATATGGTTCCAAAAA+CGG	+	chr5.4:58196211-58196230	MS.gene26216:CDS	30.0%
	GTTAACATATTAAGAACCAC+CGG	-	chr5.4:58195522-58195541	None:intergenic	30.0%
	TAGAAAAATTGCTAAGGACA+TGG	+	chr5.4:58196292-58196311	MS.gene26216:CDS	30.0%
	TGATTCATTAATCGAATGGA+CGG	+	chr5.4:58195323-58195342	MS.gene26216:CDS	30.0%
	TTCAACTTACGAAGATCTAA+TGG	+	chr5.4:58195164-58195183	MS.gene26216:CDS	30.0%
!	CTTTTGTGAGTTTGAAAGAA+GGG	+	chr5.4:58196126-58196145	MS.gene26216:CDS	30.0%
!	TACAAACTTCTGAATTTTCC+CGG	-	chr5.4:58196270-58196289	None:intergenic	30.0%
!	TTTGGTCTTTGATGAGAATA+AGG	-	chr5.4:58195104-58195123	None:intergenic	30.0%
!	TTTTGCAACGGTTTATTATC+AGG	-	chr5.4:58195552-58195571	None:intergenic	30.0%
!!!	AGAGGTTTATGTTGTTTTGA+AGG	-	chr5.4:58195081-58195100	None:intergenic	30.0%
!!!	ATATGGACAGTTTTTTGCAA+CGG	-	chr5.4:58195564-58195583	None:intergenic	30.0%
!!!	ATGTTGTTTTGAAGGGAATA+AGG	-	chr5.4:58195073-58195092	None:intergenic	30.0%
!!!	CATAGATAAATGGGTTGTTT+TGG	-	chr5.4:58195351-58195370	None:intergenic	30.0%
!!!	GAGGTTTATGTTGTTTTGAA+GGG	-	chr5.4:58195080-58195099	None:intergenic	30.0%
!!!	GTTTTTGGAACCATATAATG+CGG	-	chr5.4:58196212-58196231	None:intergenic	30.0%
	AAGGGAATAAGGATAAAGCT+TGG	-	chr5.4:58195062-58195081	None:intergenic	35.0%
	ATGTTACTCAACTACACTTC+CGG	+	chr5.4:58195240-58195259	MS.gene26216:CDS	35.0%
	GAAGAATTTGCCGCATTATA+TGG	+	chr5.4:58196199-58196218	MS.gene26216:CDS	35.0%
	GAAGTGTAGTTGAGTAACAT+TGG	-	chr5.4:58195240-58195259	None:intergenic	35.0%
	GGATAAACCCAGTCAAAATT+TGG	-	chr5.4:58195201-58195220	None:intergenic	35.0%
	GGGTATTTGAAAGATAGTGA+AGG	+	chr5.4:58195879-58195898	MS.gene26216:CDS	35.0%
	GTTGTACATGGAAGAAGAAA+TGG	+	chr5.4:58195694-58195713	MS.gene26216:CDS	35.0%
	TAAACCCTAAAGCTTCAGTA+CGG	-	chr5.4:58195626-58195645	None:intergenic	35.0%
	TAGGTGTAATGCATGAAGAT+GGG	+	chr5.4:58195946-58195965	MS.gene26216:CDS	35.0%
	TCAAATATCTCTTTCTCCGT+TGG	-	chr5.4:58196170-58196189	None:intergenic	35.0%
	TCTTTGATGAGAATAAGGAG+AGG	-	chr5.4:58195099-58195118	None:intergenic	35.0%
	TTATATGGTTCCAAAAACGG+TGG	+	chr5.4:58196214-58196233	MS.gene26216:CDS	35.0%
	TTTACAGTGAGTCATGGTTA+CGG	+	chr5.4:58195642-58195661	MS.gene26216:CDS	35.0%
!	CGTTTTACACTTTCACCATT+GGG	-	chr5.4:58195807-58195826	None:intergenic	35.0%
!	GCTTTTGTGAGTTTGAAAGA+AGG	+	chr5.4:58196125-58196144	MS.gene26216:CDS	35.0%
!	TCGTTTTACACTTTCACCAT+TGG	-	chr5.4:58195808-58195827	None:intergenic	35.0%
!	TCTTTAAACACCACCGTTTT+TGG	-	chr5.4:58196227-58196246	None:intergenic	35.0%
!	TGTTGATGTTCTTTCTAGGA+AGG	-	chr5.4:58194737-58194756	None:intergenic	35.0%
!	TTCAAACTCACAAAAGCACA+CGG	-	chr5.4:58196122-58196141	None:intergenic	35.0%
!!	CCGTTGATTCATTAATCGAA+TGG	+	chr5.4:58195319-58195338	MS.gene26216:CDS	35.0%
!!!	ACTGTCCATATTTTAACAGC+TGG	+	chr5.4:58195573-58195592	MS.gene26216:CDS	35.0%
	AAACGAGTTTGGATTCACAG+TGG	-	chr5.4:58195008-58195027	None:intergenic	40.0%
	AACTTCCTAAGACTTCTACC+GGG	+	chr5.4:58196249-58196268	MS.gene26216:CDS	40.0%
	AGAGGTGGTTGTGTTATGTT+AGG	+	chr5.4:58195858-58195877	MS.gene26216:CDS	40.0%
	AGGTAGCGTCCATAGATAAA+TGG	-	chr5.4:58195361-58195380	None:intergenic	40.0%
	ATCAACCTTTGTCATCCCTA+GGG	-	chr5.4:58195775-58195794	None:intergenic	40.0%
	CCAGTCAAAATTTGGATCAC+CGG	-	chr5.4:58195193-58195212	None:intergenic	40.0%
	CGGTTATGATGAAAGTTCCA+CGG	-	chr5.4:58195302-58195321	None:intergenic	40.0%
	CTACCTATGTTCCATGCTAA+CGG	+	chr5.4:58195375-58195394	MS.gene26216:CDS	40.0%
	CTTGGTGAAGTTGTTATGAG+AGG	+	chr5.4:58195840-58195859	MS.gene26216:CDS	40.0%
	GAAAGAAGGGTTGAAGGAAA+GGG	+	chr5.4:58196139-58196158	MS.gene26216:CDS	40.0%
	GAACTTCCTAAGACTTCTAC+CGG	+	chr5.4:58196248-58196267	MS.gene26216:CDS	40.0%
	GAAGGAAAGGGATATACCAA+CGG	+	chr5.4:58196151-58196170	MS.gene26216:CDS	40.0%
	GAGGTGGTTGTGTTATGTTA+GGG	+	chr5.4:58195859-58195878	MS.gene26216:CDS	40.0%
	GAGTTTGAAAGAAGGGTTGA+AGG	+	chr5.4:58196133-58196152	MS.gene26216:CDS	40.0%
	GATGTCAACGAAAACGAGTT+TGG	-	chr5.4:58195019-58195038	None:intergenic	40.0%
	GGTAGCGTCCATAGATAAAT+GGG	-	chr5.4:58195360-58195379	None:intergenic	40.0%
	GGTTTATCCAAACTCAGAGT+GGG	+	chr5.4:58195212-58195231	MS.gene26216:CDS	40.0%
	GTAGGTGTAATGCATGAAGA+TGG	+	chr5.4:58195945-58195964	MS.gene26216:CDS	40.0%
	GTGCTCCAGCTGTTAAAATA+TGG	-	chr5.4:58195581-58195600	None:intergenic	40.0%
	TACCTATGTTCCATGCTAAC+GGG	+	chr5.4:58195376-58195395	MS.gene26216:CDS	40.0%
	TACGAAGATCTAATGGCTTC+CGG	+	chr5.4:58195171-58195190	MS.gene26216:CDS	40.0%
	TATGATGAAAGTTCCACGGT+GGG	-	chr5.4:58195298-58195317	None:intergenic	40.0%
	TGAAAGAAGGGTTGAAGGAA+AGG	+	chr5.4:58196138-58196157	MS.gene26216:CDS	40.0%
	TGAGAATTTGAGTAGTGTGG+AGG	+	chr5.4:58196016-58196035	MS.gene26216:CDS	40.0%
	TGGTGAGAATTTGAGTAGTG+TGG	+	chr5.4:58196013-58196032	MS.gene26216:CDS	40.0%
	TTACAGTGAGTCATGGTTAC+GGG	+	chr5.4:58195643-58195662	MS.gene26216:CDS	40.0%
	TTAGGGTTTACAGTGAGTCA+TGG	+	chr5.4:58195636-58195655	MS.gene26216:CDS	40.0%
	TTATGATGAAAGTTCCACGG+TGG	-	chr5.4:58195299-58195318	None:intergenic	40.0%
!	ATTTTCCCGGTAGAAGTCTT+AGG	-	chr5.4:58196257-58196276	None:intergenic	40.0%
!	CACATACGACTTTTTGTGAC+TGG	-	chr5.4:58196335-58196354	None:intergenic	40.0%
!	GGTGAAAGTGTAAAACGAGA+TGG	+	chr5.4:58195810-58195829	MS.gene26216:CDS	40.0%
!	GGTTTGAGACCATGTGAAAA+CGG	-	chr5.4:58194797-58194816	None:intergenic	40.0%
!	TGGGAATGAACTACACCTTT+TGG	-	chr5.4:58195279-58195298	None:intergenic	40.0%
!	TTCTTTCTAGGAAGGTGAGA+GGG	-	chr5.4:58194729-58194748	None:intergenic	40.0%
!!	ATGGAATTTGTTACCAGCGA+CGG	+	chr5.4:58195713-58195732	MS.gene26216:CDS	40.0%
!!	GATGACAAAGGTTGATGTAG+TGG	+	chr5.4:58195779-58195798	MS.gene26216:CDS	40.0%
!!	GATTGGTGGTTTGTTGTACA+TGG	+	chr5.4:58195682-58195701	MS.gene26216:CDS	40.0%
!!	TAGATGATGGAAGGTGTGTT+GGG	-	chr5.4:58194766-58194785	None:intergenic	40.0%
!!	TGGTTTTACACTGGTGATGT+AGG	+	chr5.4:58195927-58195946	MS.gene26216:CDS	40.0%
!!!	AAGAAGGGTTGGTTTTACAC+TGG	+	chr5.4:58195918-58195937	MS.gene26216:CDS	40.0%
!!!	CGGTGATCCAAATTTTGACT+GGG	+	chr5.4:58195191-58195210	MS.gene26216:CDS	40.0%
	AAGTGGAGTTCATACATGGC+GGG	-	chr5.4:58194907-58194926	None:intergenic	45.0%
	AGAGAGCAAGGTTGAAGTCA+CGG	+	chr5.4:58195736-58195755	MS.gene26216:CDS	45.0%
	CATCAACCTTTGTCATCCCT+AGG	-	chr5.4:58195776-58195795	None:intergenic	45.0%
	GAAGTGGAGTTCATACATGG+CGG	-	chr5.4:58194908-58194927	None:intergenic	45.0%
	GAGTGGAGAGTTGTTTGCTT+GGG	-	chr5.4:58194704-58194723	None:intergenic	45.0%
	GATACCTAAGGAGGTGATAG+CGG	-	chr5.4:58194845-58194864	None:intergenic	45.0%
	GATGTTGTTAAGGATAGCGC+CGG	-	chr5.4:58194944-58194963	None:intergenic	45.0%
	GGGTTTATCCAAACTCAGAG+TGG	+	chr5.4:58195211-58195230	MS.gene26216:CDS	45.0%
	GGTGAAGTTGTTATGAGAGG+TGG	+	chr5.4:58195843-58195862	MS.gene26216:CDS	45.0%
	TCCACCGTACTGAAGCTTTA+GGG	+	chr5.4:58195619-58195638	MS.gene26216:CDS	45.0%
	TGAGTGGAGAGTTGTTTGCT+TGG	-	chr5.4:58194705-58194724	None:intergenic	45.0%
	TTGGAGAACAAGATCACGTG+CGG	-	chr5.4:58195043-58195062	None:intergenic	45.0%
!	ACAACGACACCGTTTTCACA+TGG	+	chr5.4:58194785-58194804	MS.gene26216:CDS	45.0%
!	GTTCTTTCTAGGAAGGTGAG+AGG	-	chr5.4:58194730-58194749	None:intergenic	45.0%
!	TCTTTCTAGGAAGGTGAGAG+GGG	-	chr5.4:58194728-58194747	None:intergenic	45.0%
!!	AACGGTGTCGTTGTAGATGA+TGG	-	chr5.4:58194779-58194798	None:intergenic	45.0%
!!	CCGGTGATCCAAATTTTGAC+TGG	+	chr5.4:58195190-58195209	MS.gene26216:CDS	45.0%
!!	GAGGTTGAGTCGGTTTTGTA+TGG	+	chr5.4:58196035-58196054	MS.gene26216:CDS	45.0%
!!	GTAGATGATGGAAGGTGTGT+TGG	-	chr5.4:58194767-58194786	None:intergenic	45.0%
!!	GTCGGTTTTGTATGGACATC+CGG	+	chr5.4:58196043-58196062	MS.gene26216:CDS	45.0%
!!	GTGTCGTTGTAGATGATGGA+AGG	-	chr5.4:58194775-58194794	None:intergenic	45.0%
!!	GTTGATGTAGTGGATCCCAA+TGG	+	chr5.4:58195789-58195808	MS.gene26216:CDS	45.0%
!!!	TTTTGGTGACGACGTTGTTC+CGG	-	chr5.4:58195262-58195281	None:intergenic	45.0%
	AAACGAGATGGAGCAACGCT+TGG	+	chr5.4:58195822-58195841	MS.gene26216:CDS	50.0%
	ACCCTAAAGCTTCAGTACGG+TGG	-	chr5.4:58195623-58195642	None:intergenic	50.0%
	AGTGGAGTTCATACATGGCG+GGG	-	chr5.4:58194906-58194925	None:intergenic	50.0%
	ATTGGGTCCCACTCTGAGTT+TGG	-	chr5.4:58195222-58195241	None:intergenic	50.0%
	CACCCGTTAGCATGGAACAT+AGG	-	chr5.4:58195381-58195400	None:intergenic	50.0%
	CATCACGTGACACACATGTG+CGG	+	chr5.4:58195492-58195511	MS.gene26216:CDS	50.0%
	CGAGTTTGGATTCACAGTGG+AGG	-	chr5.4:58195005-58195024	None:intergenic	50.0%
	CTAAGGACATGGGACCACTT+AGG	+	chr5.4:58196303-58196322	MS.gene26216:CDS	50.0%
	CTATGTTCCATGCTAACGGG+TGG	+	chr5.4:58195379-58195398	MS.gene26216:CDS	50.0%
	CTCCACCGTACTGAAGCTTT+AGG	+	chr5.4:58195618-58195637	MS.gene26216:CDS	50.0%
	GAGAACCCTAGGGATGACAA+AGG	+	chr5.4:58195767-58195786	MS.gene26216:CDS	50.0%
	GAGTAGTGTGGAGGTTGAGT+CGG	+	chr5.4:58196025-58196044	MS.gene26216:CDS	50.0%
	GGAACAACGTCGTCACCAAA+AGG	+	chr5.4:58195261-58195280	MS.gene26216:CDS	50.0%
	GGAGAGTTGTTTGCTTGGGT+TGG	-	chr5.4:58194700-58194719	None:intergenic	50.0%
	GGCGAAGTGGAGTTCATACA+TGG	-	chr5.4:58194911-58194930	None:intergenic	50.0%
	GTGACTGGATGCTTCCTAAG+TGG	-	chr5.4:58196320-58196339	None:intergenic	50.0%
	GTTTGGATTCACAGTGGAGG+AGG	-	chr5.4:58195002-58195021	None:intergenic	50.0%
!	GAGTCATGGTTACGGGCTAA+CGG	+	chr5.4:58195650-58195669	MS.gene26216:CDS	50.0%
!	GCAAGGGAGGATGAGTTTTG+GGG	+	chr5.4:58196089-58196108	MS.gene26216:CDS	50.0%
!	GGCAAGGGAGGATGAGTTTT+GGG	+	chr5.4:58196088-58196107	MS.gene26216:CDS	50.0%
!	GGCGCGTGTTGATGTTGTTA+AGG	-	chr5.4:58194954-58194973	None:intergenic	50.0%
!	TCATACATGGCGGGGATGTT+GGG	-	chr5.4:58194898-58194917	None:intergenic	50.0%
!	TGGCAAGGGAGGATGAGTTT+TGG	+	chr5.4:58196087-58196106	MS.gene26216:CDS	50.0%
!	TTCATACATGGCGGGGATGT+TGG	-	chr5.4:58194899-58194918	None:intergenic	50.0%
!!	AAGTTGGAGGCATCGTTGGT+GGG	-	chr5.4:58194818-58194837	None:intergenic	50.0%
!!	CAAGGGAGGATGAGTTTTGG+GGG	+	chr5.4:58196090-58196109	MS.gene26216:CDS	50.0%
!!	GGTTTTGTATGGACATCCGG+CGG	+	chr5.4:58196046-58196065	MS.gene26216:CDS	50.0%
!!	TTAAGGATAGCGCCGGTGAA+GGG	-	chr5.4:58194937-58194956	None:intergenic	50.0%
!!!	AAGGGAGGATGAGTTTTGGG+GGG	+	chr5.4:58196091-58196110	MS.gene26216:CDS	50.0%
	GCAAGGTTGAAGTCACGGCA+AGG	+	chr5.4:58195741-58195760	MS.gene26216:CDS	55.0%
	GCTAACGGAGACAGGTGGAT+TGG	+	chr5.4:58195665-58195684	MS.gene26216:CDS	55.0%
	GGATTCACAGTGGAGGAGGA+TGG	-	chr5.4:58194998-58195017	None:intergenic	55.0%
	GGCAAGGAGTGAGAACCCTA+GGG	+	chr5.4:58195757-58195776	MS.gene26216:CDS	55.0%
	GGTGTAGTTCATTCCCACCG+TGG	+	chr5.4:58195282-58195301	MS.gene26216:CDS	55.0%
	TGGAGATGATACGCGCGTCA+AGG	-	chr5.4:58194975-58194994	None:intergenic	55.0%
	TTCACAGTGGAGGAGGATGG+TGG	-	chr5.4:58194995-58195014	None:intergenic	55.0%
!!	AACGGAGACAGGTGGATTGG+TGG	+	chr5.4:58195668-58195687	MS.gene26216:CDS	55.0%
!!	ACCTAAGGAGGTGATAGCGG+AGG	-	chr5.4:58194842-58194861	None:intergenic	55.0%
!!	AGGCAAGTTGGAGGCATCGT+TGG	-	chr5.4:58194822-58194841	None:intergenic	55.0%
!!	AGGTGATAGCGGAGGCAAGT+TGG	-	chr5.4:58194834-58194853	None:intergenic	55.0%
!!	CAAGTTGGAGGCATCGTTGG+TGG	-	chr5.4:58194819-58194838	None:intergenic	55.0%
!!	GTTAAGGATAGCGCCGGTGA+AGG	-	chr5.4:58194938-58194957	None:intergenic	55.0%
!!	TAAGGATAGCGCCGGTGAAG+GGG	-	chr5.4:58194936-58194955	None:intergenic	55.0%
!!	TGATAGCGGAGGCAAGTTGG+AGG	-	chr5.4:58194831-58194850	None:intergenic	55.0%
	AACCTTGCTCTCTCCGTCGC+TGG	-	chr5.4:58195729-58195748	None:intergenic	60.0%
	ACATCCGGCGGTGAATGAGG+CGG	+	chr5.4:58196058-58196077	MS.gene26216:CDS	60.0%
	ACCTCCTTAGGTATCCGCCG+TGG	+	chr5.4:58194850-58194869	MS.gene26216:CDS	60.0%
	AGGAAGGTGAGAGGGGTGAG+TGG	-	chr5.4:58194721-58194740	None:intergenic	60.0%
	CAACAGCTGCAATCCCCCAC+GGG	-	chr5.4:58195410-58195429	None:intergenic	60.0%
	CCAACAGCTGCAATCCCCCA+CGG	-	chr5.4:58195411-58195430	None:intergenic	60.0%
	CGGCAAGGAGTGAGAACCCT+AGG	+	chr5.4:58195756-58195775	MS.gene26216:CDS	60.0%
	GACACACATGTGCGGTGCTC+CGG	+	chr5.4:58195500-58195519	MS.gene26216:CDS	60.0%
	GCCTCCGCTATCACCTCCTT+AGG	+	chr5.4:58194838-58194857	MS.gene26216:CDS	60.0%
	GGAAGCTCCACCCGTTAGCA+TGG	-	chr5.4:58195389-58195408	None:intergenic	60.0%
	GGGGATGTTGGGAGCAACGA+CGG	-	chr5.4:58194887-58194906	None:intergenic	60.0%
	GTTGCCACGGCGGATACCTA+AGG	-	chr5.4:58194857-58194876	None:intergenic	60.0%
	TACCAGCGACGGAGAGAGCA+AGG	+	chr5.4:58195724-58195743	MS.gene26216:CDS	60.0%
	TGGACATCCGGCGGTGAATG+AGG	+	chr5.4:58196055-58196074	MS.gene26216:CDS	60.0%
	TGGGGGATTGCAGCTGTTGG+TGG	+	chr5.4:58195411-58195430	MS.gene26216:CDS	60.0%
!	GGTTACGGGCTAACGGAGAC+AGG	+	chr5.4:58195657-58195676	MS.gene26216:CDS	60.0%
!	TAACGGGTGGAGCTTCCCGT+GGG	+	chr5.4:58195392-58195411	MS.gene26216:CDS	60.0%
!	TACGGGCTAACGGAGACAGG+TGG	+	chr5.4:58195660-58195679	MS.gene26216:CDS	60.0%
	AACGGGTGGAGCTTCCCGTG+GGG	+	chr5.4:58195393-58195412	MS.gene26216:CDS	65.0%
	ACACATGTGCGGTGCTCCGG+TGG	+	chr5.4:58195503-58195522	MS.gene26216:CDS	65.0%
	CACTTCGCCGTCCCCTTCAC+CGG	+	chr5.4:58194922-58194941	MS.gene26216:CDS	65.0%
	CCGTGGGGGATTGCAGCTGT+TGG	+	chr5.4:58195408-58195427	MS.gene26216:CDS	65.0%
	CGACGGAGACGACGTTGCCA+CGG	-	chr5.4:58194870-58194889	None:intergenic	65.0%
	GCCACGGCGGATACCTAAGG+AGG	-	chr5.4:58194854-58194873	None:intergenic	65.0%
!	CTAACGGGTGGAGCTTCCCG+TGG	+	chr5.4:58195391-58195410	MS.gene26216:CDS	65.0%
!!	GATAGCGCCGGTGAAGGGGA+CGG	-	chr5.4:58194932-58194951	None:intergenic	65.0%
	ACCGCCGCCTCATTCACCGC+CGG	-	chr5.4:58196065-58196084	None:intergenic	70.0%
	ACGGGTGGAGCTTCCCGTGG+GGG	+	chr5.4:58195394-58195413	MS.gene26216:CDS	70.0%
	CGGAGACGACGTTGCCACGG+CGG	-	chr5.4:58194867-58194886	None:intergenic	70.0%
	CGGTGAAGGGGACGGCGAAG+TGG	-	chr5.4:58194924-58194943	None:intergenic	70.0%
	GGTGAATGAGGCGGCGGTGG+TGG	+	chr5.4:58196067-58196086	MS.gene26216:CDS	70.0%
	GTACGGTGGAGAACCGCCGC+AGG	-	chr5.4:58195609-58195628	None:intergenic	70.0%
	TCCGGCGGTGAATGAGGCGG+CGG	+	chr5.4:58196061-58196080	MS.gene26216:CDS	70.0%
!	ATGAGGCGGCGGTGGTGGCA+AGG	+	chr5.4:58196072-58196091	MS.gene26216:CDS	70.0%
!	TGAGGCGGCGGTGGTGGCAA+GGG	+	chr5.4:58196073-58196092	MS.gene26216:CDS	70.0%
	CGGTGGAGAACCGCCGCAGG+CGG	-	chr5.4:58195606-58195625	None:intergenic	75.0%
	GGCGGTGAATGAGGCGGCGG+TGG	+	chr5.4:58196064-58196083	MS.gene26216:CDS	75.0%
	TGGAGAACCGCCGCAGGCGG+CGG	-	chr5.4:58195603-58195622	None:intergenic	75.0%
!!	AGCTGGAGCACCGCCGCCTG+CGG	+	chr5.4:58195590-58195609	MS.gene26216:CDS	75.0%
!!	GGCGGCGGTGGTGGCAAGGG+AGG	+	chr5.4:58196076-58196095	MS.gene26216:CDS	80.0%
!!!	TGGAGCACCGCCGCCTGCGG+CGG	+	chr5.4:58195593-58195612	MS.gene26216:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	58194682	58196355	58194682	ID=MS.gene26216
chr5.4	mRNA	58194682	58196355	58194682	ID=MS.gene26216.t1;Parent=MS.gene26216
chr5.4	exon	58194682	58196355	58194682	ID=MS.gene26216.t1.exon1;Parent=MS.gene26216.t1
chr5.4	CDS	58194682	58196355	58194682	ID=cds.MS.gene26216.t1;Parent=MS.gene26216.t1

Gene Sequence

>MS.gene26216

ATGGAACAACTAAAACCAACCCAAGCAAACAACTCTCCACTCACCCCTCTCACCTTCCTAGAAAGAACATCAACAATCTATCCCAACACACCTTCCATCATCTACAACGACACCGTTTTCACATGGTCTCAAACCCACCAACGATGCCTCCAACTTGCCTCCGCTATCACCTCCTTAGGTATCCGCCGTGGCAACGTCGTCTCCGTCGTTGCTCCCAACATCCCCGCCATGTATGAACTCCACTTCGCCGTCCCCTTCACCGGCGCTATCCTTAACAACATCAACACGCGCCTTGACGCGCGTATCATCTCCACCATCCTCCTCCACTGTGAATCCAAACTCGTTTTCGTTGACATCGCCGCACGTGATCTTGTTCTCCAAGCTTTATCCTTATTCCCTTCAAAACAACATAAACCTCTCCTTATTCTCATCAAAGACCAAACATTTGAAAATGTTTCTTCATCTTCTACCGTTGATTTTATTTCAACTTACGAAGATCTAATGGCTTCCGGTGATCCAAATTTTGACTGGGTTTATCCAAACTCAGAGTGGGACCCAATGTTACTCAACTACACTTCCGGAACAACGTCGTCACCAAAAGGTGTAGTTCATTCCCACCGTGGAACTTTCATCATAACCGTTGATTCATTAATCGAATGGACGGTTCCAAAACAACCCATTTATCTATGGACGCTACCTATGTTCCATGCTAACGGGTGGAGCTTCCCGTGGGGGATTGCAGCTGTTGGTGGAACAAACATCTGTGTAAGAAAATTCGACGCTGAAATCGTTTTTTCTCTCATCAGAAAACATCACGTGACACACATGTGCGGTGCTCCGGTGGTTCTTAATATGTTAACTAACTCACCTGATAATAAACCGTTGCAAAAAACTGTCCATATTTTAACAGCTGGAGCACCGCCGCCTGCGGCGGTTCTCCACCGTACTGAAGCTTTAGGGTTTACAGTGAGTCATGGTTACGGGCTAACGGAGACAGGTGGATTGGTGGTTTGTTGTACATGGAAGAAGAAATGGAATTTGTTACCAGCGACGGAGAGAGCAAGGTTGAAGTCACGGCAAGGAGTGAGAACCCTAGGGATGACAAAGGTTGATGTAGTGGATCCCAATGGTGAAAGTGTAAAACGAGATGGAGCAACGCTTGGTGAAGTTGTTATGAGAGGTGGTTGTGTTATGTTAGGGTATTTGAAAGATAGTGAAGGAACAAAAAATTGTTTTAAGAAGGGTTGGTTTTACACTGGTGATGTAGGTGTAATGCATGAAGATGGGTATTTAGAGATTAAAGATAGGTCAAAAGATGTTATTATTAGTGGTGGTGAGAATTTGAGTAGTGTGGAGGTTGAGTCGGTTTTGTATGGACATCCGGCGGTGAATGAGGCGGCGGTGGTGGCAAGGGAGGATGAGTTTTGGGGGGAGACACCGTGTGCTTTTGTGAGTTTGAAAGAAGGGTTGAAGGAAAGGGATATACCAACGGAGAAAGAGATATTTGAGTATTGTAGGAAGAATTTGCCGCATTATATGGTTCCAAAAACGGTGGTGTTTAAAGATGAACTTCCTAAGACTTCTACCGGGAAAATTCAGAAGTTTGTACTTAGAAAAATTGCTAAGGACATGGGACCACTTAGGAAGCATCCAGTCACAAAAAGTCGTATGTGA

Protein sequence

>MS.gene26216.t1

MEQLKPTQANNSPLTPLTFLERTSTIYPNTPSIIYNDTVFTWSQTHQRCLQLASAITSLGIRRGNVVSVVAPNIPAMYELHFAVPFTGAILNNINTRLDARIISTILLHCESKLVFVDIAARDLVLQALSLFPSKQHKPLLILIKDQTFENVSSSSTVDFISTYEDLMASGDPNFDWVYPNSEWDPMLLNYTSGTTSSPKGVVHSHRGTFIITVDSLIEWTVPKQPIYLWTLPMFHANGWSFPWGIAAVGGTNICVRKFDAEIVFSLIRKHHVTHMCGAPVVLNMLTNSPDNKPLQKTVHILTAGAPPPAAVLHRTEALGFTVSHGYGLTETGGLVVCCTWKKKWNLLPATERARLKSRQGVRTLGMTKVDVVDPNGESVKRDGATLGEVVMRGGCVMLGYLKDSEGTKNCFKKGWFYTGDVGVMHEDGYLEIKDRSKDVIISGGENLSSVEVESVLYGHPAVNEAAVVAREDEFWGETPCAFVSLKEGLKERDIPTEKEIFEYCRKNLPHYMVPKTVVFKDELPKTSTGKIQKFVLRKIAKDMGPLRKHPVTKSRM