Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045007.t1 | RHN79104.1 | 97.4 | 989 | 17 | 2 | 1 | 989 | 1 | 980 | 0.00E+00 | 1785 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045007.t1 | O81644 | 70.6 | 986 | 275 | 7 | 6 | 989 | 4 | 976 | 0.0e+00 | 1272.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045007.t1 | A0A396JPH0 | 97.4 | 989 | 17 | 2 | 1 | 989 | 1 | 980 | 0.0e+00 | 1785.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene49850 | MS.gene045007 | PPI |
| MS.gene055991 | MS.gene045007 | PPI |
| MS.gene045007 | MS.gene52664 | PPI |
| MS.gene037502 | MS.gene045007 | PPI |
| MS.gene057838 | MS.gene045007 | PPI |
| MS.gene31154 | MS.gene045007 | PPI |
| MS.gene70078 | MS.gene045007 | PPI |
| MS.gene052120 | MS.gene045007 | PPI |
| MS.gene45493 | MS.gene045007 | PPI |
| MS.gene37081 | MS.gene045007 | PPI |
| MS.gene045007 | MS.gene000108 | PPI |
| MS.gene045007 | MS.gene70072 | PPI |
| MS.gene30959 | MS.gene045007 | PPI |
| MS.gene057833 | MS.gene045007 | PPI |
| MS.gene057841 | MS.gene045007 | PPI |
| MS.gene045007 | MS.gene053110 | PPI |
| MS.gene45491 | MS.gene045007 | PPI |
| MS.gene045007 | MS.gene70077 | PPI |
| MS.gene82765 | MS.gene045007 | PPI |
| MS.gene032698 | MS.gene045007 | PPI |
| MS.gene045007 | MS.gene37274 | PPI |
| MS.gene034501 | MS.gene045007 | PPI |
| MS.gene017992 | MS.gene045007 | PPI |
| MS.gene045007 | MS.gene04628 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045007.t1 | MTR_7g091460 | 77.688 | 995 | 204 | 7 | 1 | 989 | 1 | 983 | 0.0 | 1517 |
| MS.gene045007.t1 | MTR_4g127660 | 45.155 | 1001 | 496 | 13 | 1 | 989 | 1 | 960 | 0.0 | 866 |
| MS.gene045007.t1 | MTR_2g036860 | 45.337 | 1008 | 486 | 12 | 1 | 989 | 1 | 962 | 0.0 | 850 |
| MS.gene045007.t1 | MTR_3g463530 | 42.500 | 760 | 419 | 6 | 6 | 765 | 6 | 747 | 0.0 | 634 |
| MS.gene045007.t1 | MTR_3g463530 | 39.062 | 128 | 73 | 2 | 867 | 989 | 757 | 884 | 1.24e-14 | 79.0 |
| MS.gene045007.t1 | MTR_3g463530 | 42.500 | 760 | 419 | 6 | 6 | 765 | 6 | 747 | 0.0 | 632 |
| MS.gene045007.t1 | MTR_3g463530 | 43.092 | 731 | 400 | 5 | 6 | 736 | 6 | 720 | 0.0 | 618 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045007.t1 | AT2G41740 | 69.254 | 992 | 282 | 10 | 4 | 989 | 2 | 976 | 0.0 | 1361 |
| MS.gene045007.t1 | AT2G41740 | 69.254 | 992 | 282 | 10 | 4 | 989 | 2 | 976 | 0.0 | 1361 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 69.565 | 989 | 277 | 6 | 1 | 989 | 1 | 965 | 0.0 | 1344 |
| MS.gene045007.t1 | AT3G57410 | 73.618 | 796 | 199 | 3 | 1 | 796 | 1 | 785 | 0.0 | 1217 |
| MS.gene045007.t1 | AT3G57410 | 75.412 | 728 | 171 | 1 | 1 | 728 | 1 | 720 | 0.0 | 1168 |
| MS.gene045007.t1 | AT4G30160 | 46.739 | 1012 | 478 | 14 | 1 | 989 | 1 | 974 | 0.0 | 882 |
| MS.gene045007.t1 | AT4G30160 | 46.739 | 1012 | 478 | 14 | 1 | 989 | 1 | 974 | 0.0 | 882 |
| MS.gene045007.t1 | AT4G30160 | 46.739 | 1012 | 478 | 14 | 1 | 989 | 1 | 974 | 0.0 | 882 |
| MS.gene045007.t1 | AT4G30160 | 46.327 | 1021 | 478 | 15 | 1 | 989 | 1 | 983 | 0.0 | 874 |
| MS.gene045007.t1 | AT5G57320 | 43.241 | 1006 | 510 | 12 | 1 | 989 | 1 | 962 | 0.0 | 840 |
| MS.gene045007.t1 | AT5G57320 | 43.241 | 1006 | 510 | 12 | 1 | 989 | 1 | 962 | 0.0 | 840 |
| MS.gene045007.t1 | AT2G29890 | 37.776 | 998 | 523 | 14 | 1 | 989 | 1 | 909 | 0.0 | 640 |
| MS.gene045007.t1 | AT2G29890 | 37.776 | 998 | 523 | 14 | 1 | 989 | 1 | 909 | 0.0 | 640 |
| MS.gene045007.t1 | AT2G29890 | 37.924 | 1002 | 540 | 14 | 1 | 989 | 1 | 933 | 0.0 | 639 |
| MS.gene045007.t1 | AT2G29890 | 37.800 | 1000 | 522 | 15 | 1 | 989 | 1 | 911 | 0.0 | 639 |
| MS.gene045007.t1 | AT2G29890 | 37.475 | 998 | 509 | 16 | 1 | 989 | 1 | 892 | 0.0 | 619 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8574 | gene | 980146 | 996470 | 980146 | ID=MS.gene045007 |
| 8574 | mRNA | 980146 | 996470 | 980146 | ID=MS.gene045007.t1;Parent=MS.gene045007 |
| 8574 | exon | 996391 | 996470 | 996391 | ID=MS.gene045007.t1.exon1;Parent=MS.gene045007.t1 |
| 8574 | CDS | 996391 | 996470 | 996391 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 995971 | 996070 | 995971 | ID=MS.gene045007.t1.exon2;Parent=MS.gene045007.t1 |
| 8574 | CDS | 995971 | 996070 | 995971 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 995240 | 995308 | 995240 | ID=MS.gene045007.t1.exon3;Parent=MS.gene045007.t1 |
| 8574 | CDS | 995240 | 995308 | 995240 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 994803 | 995039 | 994803 | ID=MS.gene045007.t1.exon4;Parent=MS.gene045007.t1 |
| 8574 | CDS | 994803 | 995039 | 994803 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 994556 | 994733 | 994556 | ID=MS.gene045007.t1.exon5;Parent=MS.gene045007.t1 |
| 8574 | CDS | 994556 | 994733 | 994556 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 994329 | 994461 | 994329 | ID=MS.gene045007.t1.exon6;Parent=MS.gene045007.t1 |
| 8574 | CDS | 994329 | 994461 | 994329 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 989764 | 989923 | 989764 | ID=MS.gene045007.t1.exon7;Parent=MS.gene045007.t1 |
| 8574 | CDS | 989764 | 989923 | 989764 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 989536 | 989686 | 989536 | ID=MS.gene045007.t1.exon8;Parent=MS.gene045007.t1 |
| 8574 | CDS | 989536 | 989686 | 989536 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 989357 | 989457 | 989357 | ID=MS.gene045007.t1.exon9;Parent=MS.gene045007.t1 |
| 8574 | CDS | 989357 | 989457 | 989357 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 989114 | 989272 | 989114 | ID=MS.gene045007.t1.exon10;Parent=MS.gene045007.t1 |
| 8574 | CDS | 989114 | 989272 | 989114 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 987126 | 987194 | 987126 | ID=MS.gene045007.t1.exon11;Parent=MS.gene045007.t1 |
| 8574 | CDS | 987126 | 987194 | 987126 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 986986 | 987054 | 986986 | ID=MS.gene045007.t1.exon12;Parent=MS.gene045007.t1 |
| 8574 | CDS | 986986 | 987054 | 986986 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 986767 | 986904 | 986767 | ID=MS.gene045007.t1.exon13;Parent=MS.gene045007.t1 |
| 8574 | CDS | 986767 | 986904 | 986767 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 986541 | 986669 | 986541 | ID=MS.gene045007.t1.exon14;Parent=MS.gene045007.t1 |
| 8574 | CDS | 986541 | 986669 | 986541 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 984679 | 984826 | 984679 | ID=MS.gene045007.t1.exon15;Parent=MS.gene045007.t1 |
| 8574 | CDS | 984679 | 984826 | 984679 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 984536 | 984546 | 984536 | ID=MS.gene045007.t1.exon16;Parent=MS.gene045007.t1 |
| 8574 | CDS | 984536 | 984546 | 984536 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 984275 | 984415 | 984275 | ID=MS.gene045007.t1.exon17;Parent=MS.gene045007.t1 |
| 8574 | CDS | 984275 | 984415 | 984275 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 984062 | 984193 | 984062 | ID=MS.gene045007.t1.exon18;Parent=MS.gene045007.t1 |
| 8574 | CDS | 984062 | 984193 | 984062 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 983659 | 983721 | 983659 | ID=MS.gene045007.t1.exon19;Parent=MS.gene045007.t1 |
| 8574 | CDS | 983659 | 983721 | 983659 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 982046 | 982150 | 982046 | ID=MS.gene045007.t1.exon20;Parent=MS.gene045007.t1 |
| 8574 | CDS | 982046 | 982150 | 982046 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 981412 | 981670 | 981412 | ID=MS.gene045007.t1.exon21;Parent=MS.gene045007.t1 |
| 8574 | CDS | 981412 | 981670 | 981412 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 980462 | 980685 | 980462 | ID=MS.gene045007.t1.exon22;Parent=MS.gene045007.t1 |
| 8574 | CDS | 980462 | 980685 | 980462 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| 8574 | exon | 980146 | 980259 | 980146 | ID=MS.gene045007.t1.exon23;Parent=MS.gene045007.t1 |
| 8574 | CDS | 980146 | 980259 | 980146 | ID=cds.MS.gene045007.t1;Parent=MS.gene045007.t1 |
| Gene Sequence |
| Protein sequence |
