AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57946

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57946.t1 MTR_4g129470 97.852 512 8 1 1 512 1 509 0.0 1030
MS.gene57946.t1 MTR_4g129470 97.406 424 8 1 89 512 1 421 0.0 849
MS.gene57946.t1 MTR_1g100797 59.057 403 150 4 7 401 1 396 1.00e-164 476
MS.gene57946.t1 MTR_0020s0130 58.313 403 153 5 7 401 1 396 3.82e-161 468
MS.gene57946.t1 MTR_1g100797 54.362 298 121 4 112 401 2 292 2.18e-103 316
MS.gene57946.t1 MTR_2g096760 31.188 404 216 11 7 376 1 376 1.59e-41 154
MS.gene57946.t1 MTR_4g063830 30.051 396 222 9 11 376 6 376 3.58e-36 140
MS.gene57946.t1 MTR_4g021880 27.646 463 251 12 20 449 101 512 1.53e-32 130
MS.gene57946.t1 MTR_4g021880 30.627 271 161 5 20 288 101 346 9.27e-23 100
MS.gene57946.t1 MTR_6g027840 28.279 244 129 8 54 266 246 474 1.02e-15 80.1
MS.gene57946.t1 MTR_1g089180 29.082 196 115 6 53 245 255 429 1.15e-14 76.6
MS.gene57946.t1 MTR_7g031370 28.740 254 135 9 40 290 436 646 1.48e-13 73.9
MS.gene57946.t1 MTR_7g031370 29.134 254 134 9 40 290 276 486 1.68e-13 73.6
MS.gene57946.t1 MTR_4g092760 26.295 251 132 7 20 262 27 232 1.47e-12 70.5
MS.gene57946.t1 MTR_5g043640 31.818 154 77 6 122 262 58 196 3.37e-12 67.0
MS.gene57946.t1 MTR_5g077770 29.694 229 113 9 24 249 563 746 3.91e-12 69.3
MS.gene57946.t1 MTR_7g100390 27.106 273 157 8 20 288 53 287 5.39e-12 68.6
MS.gene57946.t1 MTR_5g019105 93.103 29 2 0 239 267 28 56 6.03e-12 61.2
MS.gene57946.t1 MTR_1g115575 28.409 264 135 9 9 264 128 345 2.97e-11 66.2
MS.gene57946.t1 MTR_4g116160 26.587 252 140 8 15 262 35 245 4.64e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57946.t1 AT5G57740 65.511 519 154 12 1 509 1 504 0.0 632
MS.gene57946.t1 AT5G07270 56.635 422 175 4 7 427 1 415 4.71e-164 475
MS.gene57946.t1 AT2G28840 31.343 402 217 11 7 376 1 375 2.35e-36 140
MS.gene57946.t1 AT2G28840 31.250 336 173 10 72 376 53 361 1.73e-26 112
MS.gene57946.t1 AT5G14230 28.302 265 151 8 39 266 329 591 1.27e-15 80.5
MS.gene57946.t1 AT5G14230 28.522 291 138 10 23 262 348 619 9.55e-13 71.2
MS.gene57946.t1 AT5G14230 28.077 260 149 7 39 262 169 426 2.13e-15 79.3
MS.gene57946.t1 AT5G14230 28.522 291 138 10 23 262 188 459 1.38e-12 70.5
MS.gene57946.t1 AT2G31820 30.645 248 130 10 46 288 217 427 1.56e-14 77.0
MS.gene57946.t1 AT3G12360 31.102 254 122 9 9 257 114 319 1.03e-13 73.9
MS.gene57946.t1 AT3G12360 31.102 254 122 9 9 257 126 331 1.21e-13 73.9
MS.gene57946.t1 AT3G04710 28.251 223 123 6 55 262 283 483 5.00e-13 72.0
MS.gene57946.t1 AT5G02620 26.562 256 162 9 35 274 1 246 5.89e-13 71.6
MS.gene57946.t1 AT5G02620 26.562 256 162 9 35 274 1 246 5.89e-13 71.6
MS.gene57946.t1 AT5G02620 26.562 256 162 9 35 274 1 246 5.89e-13 71.6
MS.gene57946.t1 AT3G04710 27.753 227 127 6 51 262 55 259 1.28e-12 70.5
MS.gene57946.t1 AT1G07710 30.315 254 132 10 16 263 65 279 1.55e-12 70.5
MS.gene57946.t1 AT1G07710 30.315 254 132 10 16 263 65 279 1.55e-12 70.5
MS.gene57946.t1 AT5G02620 27.897 233 142 9 52 268 18 240 1.81e-12 70.1
MS.gene57946.t1 AT5G02620 29.885 174 93 4 23 195 98 243 6.25e-12 68.6
MS.gene57946.t1 AT5G02620 27.897 233 142 9 52 268 18 240 1.81e-12 70.1
MS.gene57946.t1 AT5G02620 29.885 174 93 4 23 195 98 243 6.25e-12 68.6
MS.gene57946.t1 AT2G03430 32.895 152 84 4 117 260 46 187 8.56e-12 65.9
MS.gene57946.t1 AT1G05640 29.536 237 129 7 54 288 191 391 8.83e-12 68.2
MS.gene57946.t1 AT1G05640 31.313 198 117 7 23 207 195 386 4.36e-11 65.9
MS.gene57946.t1 AT3G04710 27.753 227 126 7 51 262 55 258 2.76e-11 66.2
MS.gene57946.t1 AT2G14255 28.646 192 101 4 50 241 90 245 4.04e-11 65.9
MS.gene57946.t1 AT5G20350 26.210 248 136 7 20 262 35 240 8.54e-11 65.1
MS.gene57946.t1 AT5G60070 28.319 226 123 9 23 245 115 304 9.88e-11 64.7

Find 124 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGAAGGGGAAGAAAATT+TGG 0.191675 8.4:+5302448 None:intergenic
GGAACTACTATTGATTTAAT+TGG 0.211124 8.4:-5306124 MS.gene57946:intron
AGTCTGGTTGGGAATTCTTT+TGG 0.215532 8.4:-5307455 MS.gene57946:CDS
TTGGATCCACGTGAAGTTAT+TGG 0.227928 8.4:+5302467 None:intergenic
CTCCATCTGGCTGCTTTAAA+TGG 0.243273 8.4:-5306484 MS.gene57946:CDS
AGATGTTCTGGTTCTGGTTT+TGG 0.245396 8.4:-5302293 MS.gene57946:CDS
TACCAAGCATCCCTAACTTT+TGG 0.247113 8.4:-5306423 MS.gene57946:CDS
TTGACAAGATGTTCTGGTTC+TGG 0.258460 8.4:-5302299 MS.gene57946:CDS
CTTGCAAGGTATTCGACTTT+TGG 0.271778 8.4:-5307344 MS.gene57946:CDS
TGTAATATATCCCAAAAGTT+AGG 0.292151 8.4:+5306412 None:intergenic
CCTTGATGGTTGCTCGTTCA+TGG 0.299512 8.4:-5304732 MS.gene57946:CDS
GAAGTTATTGGTTTGCAAAA+TGG 0.338692 8.4:+5302479 None:intergenic
CTCAACTCTAGTCTTTGTTT+AGG 0.346862 8.4:-5302386 MS.gene57946:CDS
AACCATTTGACAAGATGTTC+TGG 0.351599 8.4:-5302305 MS.gene57946:CDS
GTTTGTGGAACATAGGTATA+AGG 0.361530 8.4:+5302697 None:intergenic
AGATCCATGTGCAGTTTGTT+TGG 0.362914 8.4:-5303776 MS.gene57946:CDS
AGCTGATGTTACTCCTTCAT+TGG 0.369879 8.4:-5302363 MS.gene57946:CDS
TTTGGTAGTGGTCTTGTGTC+TGG 0.372671 8.4:+5302590 None:intergenic
GGAGGGCAGTACCTCCATTC+AGG 0.376256 8.4:+5306503 None:intergenic
GTGTATCTCTTGCTTGAGTC+TGG 0.396912 8.4:-5307179 MS.gene57946:CDS
GTTGTCCTTTGCATTACTTT+TGG 0.399471 8.4:+5302572 None:intergenic
TCCCACCACATGCTGCATAA+TGG 0.401799 8.4:+5305266 None:intergenic
TGTGCAGTTGCTCTTGGACT+TGG 0.405253 8.4:-5306179 MS.gene57946:CDS
TGAAATTTCTAAGTCTGGTT+GGG 0.412874 8.4:-5307466 MS.gene57946:CDS
AAATGTATGGTCGCTGTAGA+AGG 0.413383 8.4:-5303748 MS.gene57946:intron
TTAGCCGAATGCACCGAGTA+TGG 0.414587 8.4:+5306461 None:intergenic
TGGAGGTACTGCCCTCCATC+TGG 0.414721 8.4:-5306497 MS.gene57946:CDS
GAACATGAAATTTCTAAGTC+TGG 0.415403 8.4:-5307471 None:intergenic
AAGCTTGCCAACATGGTCAT+TGG 0.417509 8.4:-5306684 MS.gene57946:CDS
AAGGAACGAAATCTTCTTGA+AGG 0.425532 8.4:+5302434 None:intergenic
AGCTTGCCAACATGGTCATT+GGG 0.431325 8.4:-5306683 MS.gene57946:CDS
TGAAAGTGTGCAGTTGCTCT+TGG 0.431844 8.4:-5306185 MS.gene57946:CDS
CTGAGAGAAGGAAATCATCT+TGG 0.432039 8.4:-5302253 MS.gene57946:CDS
TTGAAGATGGACTCCCTTGA+TGG 0.437882 8.4:-5304746 MS.gene57946:intron
ACGTGTTCAAGTGAAGTTCT+TGG 0.438387 8.4:-5302524 MS.gene57946:CDS
CTCGTTCATGGCATAGAAGT+TGG 0.441525 8.4:-5304720 MS.gene57946:CDS
TCTTGTTGAAGTTGAGTTTG+TGG 0.446504 8.4:+5302682 None:intergenic
ATGAAATTTCTAAGTCTGGT+TGG 0.447499 8.4:-5307467 MS.gene57946:CDS
GTGCAGTTGCTCTTGGACTT+GGG 0.447704 8.4:-5306178 MS.gene57946:CDS
AGGCATCACTGCGCTGCATA+TGG 0.449962 8.4:-5306227 MS.gene57946:CDS
ATAAGTGGAAGAGATATATA+TGG 0.450125 8.4:+5304643 None:intergenic
CTGCATATGGCAGCATTGAA+TGG 0.451201 8.4:-5306214 MS.gene57946:CDS
TTCAATCAATCCGTTTCTAC+AGG 0.452780 8.4:-5302221 MS.gene57946:CDS
CTGACTGCTGAGAATGCAAA+TGG 0.460225 8.4:-5305076 MS.gene57946:CDS
TGAACACGTGCAAAATGTTA+AGG 0.461338 8.4:+5302538 None:intergenic
TTGCATTACTCTGCAGCTCA+TGG 0.463642 8.4:-5307305 MS.gene57946:CDS
TTGTTTGGAAAGGAAATGTA+TGG 0.464104 8.4:-5303761 MS.gene57946:CDS
ATGGCCATACTCGGTGCATT+CGG 0.469289 8.4:-5306465 MS.gene57946:CDS
AAGTTGAGTTTGTGGAACAT+AGG 0.477507 8.4:+5302690 None:intergenic
GCACATGGATCTAAGCATAT+AGG 0.478105 8.4:+5303787 None:intergenic
CTTTCCAAACAAACTGCACA+TGG 0.481712 8.4:+5303772 None:intergenic
AACCAGAACATCTTGTCAAA+TGG 0.484480 8.4:+5302303 None:intergenic
CAATCCGTTTCTACAGGAAG+TGG 0.491465 8.4:-5302215 MS.gene57946:CDS
GCATCCACTTCCTGTAGAAA+CGG 0.494458 8.4:+5302211 None:intergenic
TCTCAGGAATTATCGTGGTC+AGG 0.496842 8.4:-5307144 MS.gene57946:intron
GTAATATATCCCAAAAGTTA+GGG 0.497275 8.4:+5306413 None:intergenic
TCCCAAAAGTTAGGGATGCT+TGG 0.498090 8.4:+5306421 None:intergenic
TGTAGAAACGGATTGATTGA+AGG 0.498824 8.4:+5302223 None:intergenic
CACAAAACAGATTACCTGAA+TGG 0.504700 8.4:-5306517 MS.gene57946:CDS
CATGTGCAGTTTGTTTGGAA+AGG 0.507634 8.4:-5303771 MS.gene57946:CDS
CTGGTGTTGATATTAATCTC+AGG 0.518171 8.4:-5307160 MS.gene57946:CDS
CCAACATGGTCATTGGGAGG+TGG 0.519366 8.4:-5306677 MS.gene57946:CDS
TTCCTAACAGAGAATGTGGC+TGG 0.520240 8.4:-5303828 MS.gene57946:intron
GTAATTAACAAAACTTCTGA+TGG 0.523746 8.4:-5306250 MS.gene57946:CDS
CTGCTTTAAATGGCCATACT+CGG 0.529232 8.4:-5306474 MS.gene57946:CDS
AGCAGTCAGACTAGCACCCT+TGG 0.529380 8.4:+5305090 None:intergenic
TCTTCAGCTATTGATTGCCA+AGG 0.531671 8.4:-5305107 MS.gene57946:intron
CTTGTGCAAGGAACCAATGA+AGG 0.533363 8.4:+5302350 None:intergenic
CTCCATTATGCAGCATGTGG+TGG 0.535401 8.4:-5305268 MS.gene57946:CDS
TGAAGAGAATTGACAACTCA+AGG 0.535842 8.4:+5302415 None:intergenic
AGAGGACAAGCTATTGAACC+TGG 0.537680 8.4:+5302647 None:intergenic
CATTTAAAGCAGCCAGATGG+AGG 0.538004 8.4:+5306485 None:intergenic
TCCATTATGCAGCATGTGGT+GGG 0.544452 8.4:-5305267 MS.gene57946:CDS
AGGAACGAAATCTTCTTGAA+GGG 0.546898 8.4:+5302435 None:intergenic
TGAGCATTGTCAAAATTGCT+AGG 0.550320 8.4:-5304621 MS.gene57946:intron
CTAGTTTCTGCAGCAAGAGA+TGG 0.552390 8.4:-5307410 MS.gene57946:CDS
GGAAGAGATATATATGGCGA+AGG 0.561512 8.4:+5304649 None:intergenic
CTTCAGCTATTGATTGCCAA+GGG 0.565183 8.4:-5305106 MS.gene57946:intron
AATATAATCCACGTCTTGCA+AGG 0.572310 8.4:-5307358 MS.gene57946:CDS
CCATGAACGAGCAACCATCA+AGG 0.576241 8.4:+5304732 None:intergenic
CTTCTATTAGCACTTGTGCA+AGG 0.580799 8.4:+5302338 None:intergenic
CAAGTTCTCAAACTGAGAGA+AGG 0.581676 8.4:-5302265 MS.gene57946:CDS
ACTATACTGTGGCGACATAG+AGG 0.581953 8.4:+5302629 None:intergenic
TGCAAACCAATAACTTCACG+TGG 0.583478 8.4:-5302473 MS.gene57946:CDS
AATAGTAGTTCCATCCTCTA+CGG 0.584436 8.4:+5306135 None:intergenic
GGCCATTTAAAGCAGCCAGA+TGG 0.585213 8.4:+5306482 None:intergenic
GTCGAATACCTTGCAAGACG+TGG 0.585836 8.4:+5307350 None:intergenic
CATGAACGAGCAACCATCAA+GGG 0.589970 8.4:+5304733 None:intergenic
CTTGGGAGCTTCTGTGTCCG+AGG 0.591035 8.4:-5306161 MS.gene57946:CDS
TCCGAGGTTACCGTAGAGGA+TGG 0.591591 8.4:-5306145 MS.gene57946:CDS
TGGTGATGTTCAAGAGGCGA+AGG 0.592755 8.4:-5307390 MS.gene57946:CDS
TCAATTCCTAACAGAGAATG+TGG 0.598490 8.4:-5303832 MS.gene57946:intron
TTGATGCAAGCTTGCCAACA+TGG 0.601264 8.4:-5306691 MS.gene57946:CDS
TGCTTGGTATATAGTCTGCA+AGG 0.603623 8.4:+5306437 None:intergenic
CACTACCAAAAGTAATGCAA+AGG 0.606802 8.4:-5302577 MS.gene57946:CDS
ATTAACAAAACTTCTGATGG+AGG 0.611011 8.4:-5306247 MS.gene57946:CDS
GAGGACAAGCTATTGAACCT+GGG 0.612469 8.4:+5302648 None:intergenic
CCACCTCCCAATGACCATGT+TGG 0.614473 8.4:+5306677 None:intergenic
TCCATCCTCTACGGTAACCT+CGG 0.615721 8.4:+5306144 None:intergenic
GGAACGAAATCTTCTTGAAG+GGG 0.616699 8.4:+5302436 None:intergenic
AAGAGATGGTGATGTTCAAG+AGG 0.619180 8.4:-5307396 MS.gene57946:CDS
CAATCTCAGAATTTGATCAA+AGG 0.622386 8.4:-5306372 MS.gene57946:intron
ATGGCATAGAAGTTGGCTTG+AGG 0.627590 8.4:-5304713 MS.gene57946:CDS
TGTGTCCGAGGTTACCGTAG+AGG 0.631676 8.4:-5306149 MS.gene57946:CDS
TGACTGCTGAGAATGCAAAT+GGG 0.638673 8.4:-5305075 MS.gene57946:intron
CATACGAAGTGGTGTTTGTG+AGG 0.641986 8.4:-5306272 MS.gene57946:intron
ATTTAAAGCAGCCAGATGGA+GGG 0.649786 8.4:+5306486 None:intergenic
TCAACTTCAACAAGAACACA+TGG 0.651528 8.4:-5302674 MS.gene57946:CDS
AGGACAAGCTATTGAACCTG+GGG 0.651920 8.4:+5302649 None:intergenic
CCACTCCATTATGCAGCATG+TGG 0.652184 8.4:-5305271 MS.gene57946:CDS
AAATGGTGTTGTCATGTCTG+TGG 0.653781 8.4:+5302496 None:intergenic
GTGCTAATAGAAGAAACTTG+AGG 0.655442 8.4:-5302328 MS.gene57946:CDS
TGAGCTGCAGAGTAATGCAA+AGG 0.656866 8.4:+5307308 None:intergenic
CCACATGCTGCATAATGGAG+TGG 0.671484 8.4:+5305271 None:intergenic
ACGTGGATTATATTCTAACA+AGG 0.672327 8.4:+5307367 None:intergenic
TTGCCAACATGGTCATTGGG+AGG 0.672352 8.4:-5306680 MS.gene57946:CDS
AAAACAGATTACCTGAATGG+AGG 0.674826 8.4:-5306514 MS.gene57946:CDS
ATTAATCTCAGGAATTATCG+TGG 0.678184 8.4:-5307149 MS.gene57946:CDS
GCAACTGCACACTTTCAACA+TGG 0.678568 8.4:+5306191 None:intergenic
TGCAGCTCATGGCCACCATG+AGG 0.682766 8.4:-5307294 MS.gene57946:intron
CTAACAGAGAATGTGGCTGG+AGG 0.704497 8.4:-5303825 MS.gene57946:intron
GGACAAGCTATTGAACCTGG+GGG 0.729015 8.4:+5302650 None:intergenic
TGTAAGTATATACCTCATGG+TGG 0.729691 8.4:+5307282 None:intergenic
TAACAAATGAGACTATACTG+TGG 0.749737 8.4:+5302618 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTACATATATTAAGTAAT+TGG - chr8.4:5306167-5306186 MS.gene57946:CDS 10.0%
!! TACTCAAATTTATATTTATT+TGG - chr8.4:5305393-5305412 MS.gene57946:intron 10.0%
!! AACATTTATAGAATTCAATT+AGG - chr8.4:5304245-5304264 MS.gene57946:intron 15.0%
!! AAGGTTATTAATTAAATGTT+AGG + chr8.4:5305125-5305144 None:intergenic 15.0%
!! AATATAGTAAATGTTTAGAT+TGG + chr8.4:5304011-5304030 None:intergenic 15.0%
!! ACATTAGAAATTATTGTTAT+TGG - chr8.4:5306834-5306853 MS.gene57946:intron 15.0%
!! ACATTGAAACAAAATAATAT+TGG + chr8.4:5302561-5302580 None:intergenic 15.0%
!! ATATAGTAAATGTTTAGATT+GGG + chr8.4:5304010-5304029 None:intergenic 15.0%
!! TAAAAACTTCATAATCTAAT+AGG + chr8.4:5305673-5305692 None:intergenic 15.0%
!! TTAATAAAATAGCACAAAAT+CGG + chr8.4:5303072-5303091 None:intergenic 15.0%
!!! TAAAATATTGCTTGTTAAAT+TGG - chr8.4:5303125-5303144 MS.gene57946:intron 15.0%
!!! TATTAAAACAATTTTTCACA+TGG + chr8.4:5305195-5305214 None:intergenic 15.0%
!! ACAATAATTTCTAATGTCAT+AGG + chr8.4:5306831-5306850 None:intergenic 20.0%
!! AGATATAGTCAACTAAATTA+AGG + chr8.4:5302590-5302609 None:intergenic 20.0%
!! AGCAAATAATTAGAAAGTAA+TGG - chr8.4:5306241-5306260 MS.gene57946:CDS 20.0%
!! ATAACTAACTTACTTTACAA+TGG - chr8.4:5303821-5303840 MS.gene57946:CDS 20.0%
!! ATTAAATGTTAGGAAGAAAT+TGG + chr8.4:5305115-5305134 None:intergenic 20.0%
!! TATAGTAAATGTTTAGATTG+GGG + chr8.4:5304009-5304028 None:intergenic 20.0%
!! TGAACAAATATAGCATTATT+TGG + chr8.4:5305644-5305663 None:intergenic 20.0%
!! TTATGATCTATTTAGATCAT+AGG - chr8.4:5304707-5304726 MS.gene57946:CDS 20.0%
!!! AATTTCTACTAGAGTATTTT+GGG + chr8.4:5306627-5306646 None:intergenic 20.0%
!!! ATATGATAAATTGATAATGC+AGG - chr8.4:5306926-5306945 MS.gene57946:intron 20.0%
!!! ATGTTTTTTCCATTTGTTTT+TGG + chr8.4:5306287-5306306 None:intergenic 20.0%
!!! ATTGGCTTTAAAAATAATCA+GGG + chr8.4:5303030-5303049 None:intergenic 20.0%
!!! TAGTATCGTAGAATTTTATT+CGG + chr8.4:5305364-5305383 None:intergenic 20.0%
!!! TATTGATTTTGTTTTGAAGA+TGG - chr8.4:5304916-5304935 MS.gene57946:intron 20.0%
!!! TATTTTTATTTACAGGTTCT+GGG - chr8.4:5304375-5304394 MS.gene57946:intron 20.0%
!!! TCAAATTTTATGTATTCCAA+AGG - chr8.4:5305779-5305798 MS.gene57946:intron 20.0%
!!! TGCTATCTATTTTTATTTAC+AGG - chr8.4:5304368-5304387 MS.gene57946:intron 20.0%
! AAAATGAAACAGTCTTCTAA+AGG + chr8.4:5304467-5304486 None:intergenic 25.0%
! AAAATGCTTCATGTTTAAAG+CGG - chr8.4:5304312-5304331 MS.gene57946:intron 25.0%
! AACTGATTATACTTTGAATG+CGG - chr8.4:5305313-5305332 MS.gene57946:intron 25.0%
! AAGTTAATTCTGTCTTTGAA+TGG - chr8.4:5303377-5303396 MS.gene57946:intron 25.0%
! AATGTGTAGAATGTAGATAA+TGG + chr8.4:5304117-5304136 None:intergenic 25.0%
! AATTGTAAGTATATACCTCA+TGG + chr8.4:5302399-5302418 None:intergenic 25.0%
! ACACTAAACCAATATTGTTA+AGG - chr8.4:5306791-5306810 MS.gene57946:intron 25.0%
! AGATCAAACTAGTTAACTTA+GGG + chr8.4:5304340-5304359 None:intergenic 25.0%
! AGATCAATAATAGTAACCTT+TGG + chr8.4:5305798-5305817 None:intergenic 25.0%
! ATAAGTGGAAGAGATATATA+TGG + chr8.4:5305035-5305054 None:intergenic 25.0%
! ATAGTAAATGTTTAGATTGG+GGG + chr8.4:5304008-5304027 None:intergenic 25.0%
! CATATATTAAGTAATTGGTC+AGG - chr8.4:5306172-5306191 MS.gene57946:CDS 25.0%
! CATCAATATTGTTCAACATT+TGG - chr8.4:5303760-5303779 MS.gene57946:CDS 25.0%
! GAATAAAATTCTACGATACT+AGG - chr8.4:5305363-5305382 MS.gene57946:intron 25.0%
! GTAAAATTATAAACCAGTAG+AGG + chr8.4:5306542-5306561 None:intergenic 25.0%
! GTAATATATCCCAAAAGTTA+GGG + chr8.4:5303265-5303284 None:intergenic 25.0%
! GTAATTAACAAAACTTCTGA+TGG - chr8.4:5303425-5303444 MS.gene57946:intron 25.0%
! TAAAAATAGATAGCAGTTAG+TGG + chr8.4:5304363-5304382 None:intergenic 25.0%
! TAACCTTTCAAGATTGATAT+TGG - chr8.4:5305138-5305157 MS.gene57946:intron 25.0%
! TAGATGAGTTTAGTCATTTA+AGG - chr8.4:5305537-5305556 MS.gene57946:intron 25.0%
! TCGTAGTAAGTTAAAAACAA+GGG + chr8.4:5304751-5304770 None:intergenic 25.0%
! TGAACAATATTGATGAGAAA+TGG + chr8.4:5303755-5303774 None:intergenic 25.0%
! TGTAATATATCCCAAAAGTT+AGG + chr8.4:5303266-5303285 None:intergenic 25.0%
! TTGCCAATAAAAATACAAAG+AGG + chr8.4:5306584-5306603 None:intergenic 25.0%
!! AGGAAGAACTTGTAAATTTT+CGG + chr8.4:5305009-5305028 None:intergenic 25.0%
!! ATTTTCTCTTATGTAGTACA+GGG + chr8.4:5304297-5304316 None:intergenic 25.0%
!! TCTACTTTGAATGTATTTTC+TGG + chr8.4:5306083-5306102 None:intergenic 25.0%
!! TGAGGATAAATAAACAGTTT+TGG + chr8.4:5304039-5304058 None:intergenic 25.0%
!! TTAGAAGACTGTTTCATTTT+AGG - chr8.4:5304467-5304486 MS.gene57946:intron 25.0%
!! TTGGATATGTTTTCTACAAA+TGG - chr8.4:5306196-5306215 MS.gene57946:CDS 25.0%
!! TTTTACATGGAAAAATGATC+TGG - chr8.4:5305992-5306011 MS.gene57946:intron 25.0%
!!! AGCATCATTTGATTTTAACA+AGG - chr8.4:5305221-5305240 MS.gene57946:intron 25.0%
!!! ATGTAGATAATGGAATATGA+TGG + chr8.4:5304107-5304126 None:intergenic 25.0%
!!! ATTTTGCAAGTGTTTCTATA+TGG - chr8.4:5306377-5306396 MS.gene57946:CDS 25.0%
!!! CAATTTCTACTAGAGTATTT+TGG + chr8.4:5306628-5306647 None:intergenic 25.0%
!!! CATTGGCTTTAAAAATAATC+AGG + chr8.4:5303031-5303050 None:intergenic 25.0%
!!! CTACCTCTTTGTATTTTTAT+TGG - chr8.4:5306578-5306597 MS.gene57946:intron 25.0%
!!! CTATTTTTATTTACAGGTTC+TGG - chr8.4:5304374-5304393 MS.gene57946:intron 25.0%
!!! CTTCAAACTTTTGTTGTTTT+GGG - chr8.4:5302879-5302898 MS.gene57946:intron 25.0%
!!! GGAACTACTATTGATTTAAT+TGG - chr8.4:5303551-5303570 MS.gene57946:intron 25.0%
AAACACGATACAAGAAAATG+AGG + chr8.4:5302429-5302448 None:intergenic 30.0%
AACCTTTGAATCAATGGTAA+AGG - chr8.4:5306333-5306352 MS.gene57946:intron 30.0%
AACTTACCTTATATACAGCT+TGG - chr8.4:5304665-5304684 MS.gene57946:CDS 30.0%
AAGAGGTAGATGACAAAATA+TGG + chr8.4:5306567-5306586 None:intergenic 30.0%
AAGTTAAAAACAAGGGGTAA+AGG + chr8.4:5304744-5304763 None:intergenic 30.0%
AATAAAAACTGAGCAATGTC+TGG + chr8.4:5306435-5306454 None:intergenic 30.0%
AATGACAACCTTTGAATCAA+TGG - chr8.4:5306327-5306346 MS.gene57946:intron 30.0%
ACATAACTATGCTTCTTTGT+TGG - chr8.4:5306303-5306322 MS.gene57946:intron 30.0%
AGAGGTAGATGACAAAATAT+GGG + chr8.4:5306566-5306585 None:intergenic 30.0%
AGATTCACTATCTATCGTAT+TGG - chr8.4:5304770-5304789 MS.gene57946:intron 30.0%
ATCACTTAATCACATGAACT+AGG + chr8.4:5303593-5303612 None:intergenic 30.0%
ATGAAATTTCTAAGTCTGGT+TGG - chr8.4:5302208-5302227 MS.gene57946:CDS 30.0%
ATGTTTCTTTCGTTTACAGA+GGG - chr8.4:5302915-5302934 MS.gene57946:intron 30.0%
ATTAACAAAACTTCTGATGG+AGG - chr8.4:5303428-5303447 MS.gene57946:intron 30.0%
ATTAATCTCAGGAATTATCG+TGG - chr8.4:5302526-5302545 MS.gene57946:CDS 30.0%
ATTTAGAAGTAGGTGAAAGA+GGG + chr8.4:5304203-5304222 None:intergenic 30.0%
CAACCAATATCAATCTTGAA+AGG + chr8.4:5305144-5305163 None:intergenic 30.0%
CAAGCACTATGTATCTTAAA+TGG - chr8.4:5304870-5304889 MS.gene57946:intron 30.0%
CAATCTCAGAATTTGATCAA+AGG - chr8.4:5303303-5303322 MS.gene57946:intron 30.0%
CATGTGATTATGTGCATATT+TGG - chr8.4:5305424-5305443 MS.gene57946:intron 30.0%
CGTAGTAAGTTAAAAACAAG+GGG + chr8.4:5304750-5304769 None:intergenic 30.0%
CTCGTAGTAAGTTAAAAACA+AGG + chr8.4:5304752-5304771 None:intergenic 30.0%
CTTACTGCAACAGTTTATAA+TGG + chr8.4:5303360-5303379 None:intergenic 30.0%
CTTAGTAAACATTAGACAAG+AGG + chr8.4:5304150-5304169 None:intergenic 30.0%
GAGATCAAACTAGTTAACTT+AGG + chr8.4:5304341-5304360 None:intergenic 30.0%
GAGTTAGAGATCTTCAATAT+AGG - chr8.4:5305449-5305468 MS.gene57946:intron 30.0%
GATCAAACTAGTTAACTTAG+GGG + chr8.4:5304339-5304358 None:intergenic 30.0%
GTTTAGTGTTTGAATTGACA+AGG + chr8.4:5306780-5306799 None:intergenic 30.0%
TAACAAATGAGACTATACTG+TGG + chr8.4:5307060-5307079 None:intergenic 30.0%
TAACTTACTTTACAATGGCT+TGG - chr8.4:5303826-5303845 MS.gene57946:intron 30.0%
TACTGCTAAGATTTAGAAGT+AGG + chr8.4:5304213-5304232 None:intergenic 30.0%
TAGTAAATGTTTAGATTGGG+GGG + chr8.4:5304007-5304026 None:intergenic 30.0%
TGAAATTTCTAAGTCTGGTT+GGG - chr8.4:5302209-5302228 MS.gene57946:CDS 30.0%
TGGCACAATAAAATTTGTAG+TGG - chr8.4:5303846-5303865 MS.gene57946:intron 30.0%
TTCTATATGGAGTTATTGCA+CGG - chr8.4:5306390-5306409 MS.gene57946:CDS 30.0%
TTGCAAAATACGTTTCTCTA+TGG + chr8.4:5306366-5306385 None:intergenic 30.0%
TTGCAGTAATGATCTTACAT+TGG + chr8.4:5303048-5303067 None:intergenic 30.0%
TTGTTTGGAAAGGAAATGTA+TGG - chr8.4:5305914-5305933 MS.gene57946:intron 30.0%
! ACGTGGATTATATTCTAACA+AGG + chr8.4:5302311-5302330 None:intergenic 30.0%
! CATTTTCTCTTATGTAGTAC+AGG + chr8.4:5304298-5304317 None:intergenic 30.0%
! GAAATTGGTTGAATTTTGAG+AGG + chr8.4:5305100-5305119 None:intergenic 30.0%
! GTGTACGTTTGTTAAAATCA+TGG - chr8.4:5303954-5303973 MS.gene57946:intron 30.0%
! TACGTTTGTTAAAATCATGG+TGG - chr8.4:5303957-5303976 MS.gene57946:intron 30.0%
! TATAGGTTTTGCCAATACTT+TGG - chr8.4:5305466-5305485 MS.gene57946:intron 30.0%
! TGCTAGATCAATGTTTTCAA+AGG + chr8.4:5302813-5302832 None:intergenic 30.0%
! TTATTTTCCATGAACGTGTT+TGG - chr8.4:5306450-5306469 MS.gene57946:CDS 30.0%
! TTTTCAAATTGTGCCTATAC+CGG - chr8.4:5302739-5302758 MS.gene57946:intron 30.0%
! TTTTCTCTTATGTAGTACAG+GGG + chr8.4:5304296-5304315 None:intergenic 30.0%
!! ATTTTGACAATGCTCATAAG+TGG + chr8.4:5305050-5305069 None:intergenic 30.0%
!! CTGATATAGATGGTTTTACA+TGG - chr8.4:5305979-5305998 MS.gene57946:intron 30.0%
!! CTTTTGGAAACTTGCATAAT+TGG - chr8.4:5303698-5303717 MS.gene57946:intron 30.0%
!! GAAGTTATTGGTTTGCAAAA+TGG + chr8.4:5307199-5307218 None:intergenic 30.0%
!! GATAATGGAATATGATGGAA+AGG + chr8.4:5304102-5304121 None:intergenic 30.0%
!! GTGCTTCTTCATTTTTCTTT+TGG - chr8.4:5303682-5303701 MS.gene57946:intron 30.0%
!! GTTATTGGCTTAAAAAGTGT+TGG - chr8.4:5306849-5306868 MS.gene57946:intron 30.0%
!! TTCCTTTACCATTGATTCAA+AGG + chr8.4:5306338-5306357 None:intergenic 30.0%
!!! GCTTCAAACTTTTGTTGTTT+TGG - chr8.4:5302878-5302897 MS.gene57946:intron 30.0%
!!! TTTTGGGATATATTACAGAC+AGG - chr8.4:5303269-5303288 MS.gene57946:intron 30.0%
AAAACAGATTACCTGAATGG+AGG - chr8.4:5303161-5303180 MS.gene57946:intron 35.0%
AACATGCCAAGCTGTATATA+AGG + chr8.4:5304674-5304693 None:intergenic 35.0%
AACCAGAACATCTTGTCAAA+TGG + chr8.4:5307375-5307394 None:intergenic 35.0%
AACCATTTGACAAGATGTTC+TGG - chr8.4:5307370-5307389 MS.gene57946:CDS 35.0%
AAGGAACGAAATCTTCTTGA+AGG + chr8.4:5307244-5307263 None:intergenic 35.0%
AAGTTGAGTTTGTGGAACAT+AGG + chr8.4:5306988-5307007 None:intergenic 35.0%
AATAGTAGTTCCATCCTCTA+CGG + chr8.4:5303543-5303562 None:intergenic 35.0%
AATATAATCCACGTCTTGCA+AGG - chr8.4:5302317-5302336 MS.gene57946:CDS 35.0%
AATATTGATGAGAAATGGCC+AGG + chr8.4:5303750-5303769 None:intergenic 35.0%
ACACTCAATGCTGATATAGA+TGG - chr8.4:5305969-5305988 MS.gene57946:intron 35.0%
AGGAACGAAATCTTCTTGAA+GGG + chr8.4:5307243-5307262 None:intergenic 35.0%
ATAACAACATACGCAAGAAG+AGG + chr8.4:5305824-5305843 None:intergenic 35.0%
ATATTGATGAGAAATGGCCA+GGG + chr8.4:5303749-5303768 None:intergenic 35.0%
ATCTCTCCAAACCAAAGTAT+TGG + chr8.4:5305480-5305499 None:intergenic 35.0%
ATTAAGTAATTGGTCAGGCT+TGG - chr8.4:5306177-5306196 MS.gene57946:CDS 35.0%
ATTCAACTGCAACTCATGTT+TGG - chr8.4:5303874-5303893 MS.gene57946:intron 35.0%
CACAAAACAGATTACCTGAA+TGG - chr8.4:5303158-5303177 MS.gene57946:intron 35.0%
CACTACCAAAAGTAATGCAA+AGG - chr8.4:5307098-5307117 MS.gene57946:intron 35.0%
CATGTTTCTTTCGTTTACAG+AGG - chr8.4:5302914-5302933 MS.gene57946:intron 35.0%
CCAAACATGCATTGAATTGA+TGG + chr8.4:5306661-5306680 None:intergenic 35.0%
CCATCAATTCAATGCATGTT+TGG - chr8.4:5306658-5306677 MS.gene57946:CDS 35.0%
CTCTGTAAACGAAAGAAACA+TGG + chr8.4:5302916-5302935 None:intergenic 35.0%
GAGCAGAGTCAATAAATTCA+TGG + chr8.4:5305257-5305276 None:intergenic 35.0%
GATTTAGAAGTAGGTGAAAG+AGG + chr8.4:5304204-5304223 None:intergenic 35.0%
GGTATGACAAAATTGTGCAT+AGG + chr8.4:5306759-5306778 None:intergenic 35.0%
GTGCTAATAGAAGAAACTTG+AGG - chr8.4:5307347-5307366 MS.gene57946:CDS 35.0%
GTTCACACACCAAAAACAAA+TGG - chr8.4:5306275-5306294 MS.gene57946:intron 35.0%
GTTTGTGGAACATAGGTATA+AGG + chr8.4:5306981-5307000 None:intergenic 35.0%
TAACAACATACGCAAGAAGA+GGG + chr8.4:5305823-5305842 None:intergenic 35.0%
TCAACAAAATCACGTTGTCA+TGG + chr8.4:5306709-5306728 None:intergenic 35.0%
TCAACTTCAACAAGAACACA+TGG - chr8.4:5307001-5307020 MS.gene57946:intron 35.0%
TCAATTCCTAACAGAGAATG+TGG - chr8.4:5305843-5305862 MS.gene57946:intron 35.0%
TCTAATGTCATAGGTCTATG+AGG + chr8.4:5306822-5306841 None:intergenic 35.0%
TCTTGAAGGGGAAGAAAATT+TGG + chr8.4:5307230-5307249 None:intergenic 35.0%
TCTTGTTGAAGTTGAGTTTG+TGG + chr8.4:5306996-5307015 None:intergenic 35.0%
TGAACACGTGCAAAATGTTA+AGG + chr8.4:5307140-5307159 None:intergenic 35.0%
TGAAGAGAATTGACAACTCA+AGG + chr8.4:5307263-5307282 None:intergenic 35.0%
TGTAAGTATATACCTCATGG+TGG + chr8.4:5302396-5302415 None:intergenic 35.0%
TTCAATCAATCCGTTTCTAC+AGG - chr8.4:5307454-5307473 MS.gene57946:CDS 35.0%
! AGAACTTGTAAATTTTCGGC+TGG + chr8.4:5305005-5305024 None:intergenic 35.0%
! AGGTGTCACCTTAACAATAT+TGG + chr8.4:5306802-5306821 None:intergenic 35.0%
! ATGTTTTCAAAGGCAAATCG+CGG + chr8.4:5302803-5302822 None:intergenic 35.0%
! CTCAACTCTAGTCTTTGTTT+AGG - chr8.4:5307289-5307308 MS.gene57946:intron 35.0%
! GAAAAATGAAGAAGCACACA+TGG + chr8.4:5303679-5303698 None:intergenic 35.0%
! GTTGTCCTTTGCATTACTTT+TGG + chr8.4:5307106-5307125 None:intergenic 35.0%
! GTTTTGCCAATACTTTGGTT+TGG - chr8.4:5305471-5305490 MS.gene57946:intron 35.0%
! TGAGCATTGTCAAAATTGCT+AGG - chr8.4:5305054-5305073 MS.gene57946:intron 35.0%
!! AGATACTTGCTTTGGAAAGT+CGG - chr8.4:5303616-5303635 MS.gene57946:intron 35.0%
!! CTGGTGTTGATATTAATCTC+AGG - chr8.4:5302515-5302534 MS.gene57946:CDS 35.0%
!! CTTTGCATTACTTTTGGTAG+TGG + chr8.4:5307100-5307119 None:intergenic 35.0%
!! TGTAGAAACGGATTGATTGA+AGG + chr8.4:5307455-5307474 None:intergenic 35.0%
!! TTGGTTGAATTTTGAGAGGA+TGG + chr8.4:5305096-5305115 None:intergenic 35.0%
!!! GTATTTTGGGTTCTAATGTG+TGG + chr8.4:5306614-5306633 None:intergenic 35.0%
AAAAACTGAGCAATGTCTGG+TGG + chr8.4:5306432-5306451 None:intergenic 40.0%
AAATGTATGGTCGCTGTAGA+AGG - chr8.4:5305927-5305946 MS.gene57946:intron 40.0%
AATCAGTTAAATCCACCTGC+TGG + chr8.4:5305301-5305320 None:intergenic 40.0%
ACGTGTTCAAGTGAAGTTCT+TGG - chr8.4:5307151-5307170 MS.gene57946:CDS 40.0%
ACTGCAACTCATGTTTGGAT+TGG - chr8.4:5303879-5303898 MS.gene57946:intron 40.0%
AGCTGATGTTACTCCTTCAT+TGG - chr8.4:5307312-5307331 MS.gene57946:CDS 40.0%
ATGTTTACTAAGCACAGCCT+TGG - chr8.4:5304158-5304177 MS.gene57946:intron 40.0%
ATTGCAATGTCAAGGAAGCT+TGG - chr8.4:5306049-5306068 MS.gene57946:intron 40.0%
CAAGTTCTCAAACTGAGAGA+AGG - chr8.4:5307410-5307429 MS.gene57946:CDS 40.0%
CACAACAGATTGATGGAGTT+TGG - chr8.4:5302673-5302692 MS.gene57946:CDS 40.0%
CTGAGAGAAGGAAATCATCT+TGG - chr8.4:5307422-5307441 MS.gene57946:CDS 40.0%
CTTCTATTAGCACTTGTGCA+AGG + chr8.4:5307340-5307359 None:intergenic 40.0%
CTTTCCAAACAAACTGCACA+TGG + chr8.4:5305906-5305925 None:intergenic 40.0%
GCACATGGATCTAAGCATAT+AGG + chr8.4:5305891-5305910 None:intergenic 40.0%
GCTATAGCACAACAGATTGA+TGG - chr8.4:5302666-5302685 MS.gene57946:CDS 40.0%
GGAAACAATCGAGAGATAGT+AGG + chr8.4:5303652-5303671 None:intergenic 40.0%
GGAACGAAATCTTCTTGAAG+GGG + chr8.4:5307242-5307261 None:intergenic 40.0%
GGAAGAGATATATATGGCGA+AGG + chr8.4:5305029-5305048 None:intergenic 40.0%
GTGATGTCAGATACTTGCTT+TGG - chr8.4:5303608-5303627 MS.gene57946:intron 40.0%
GTGCAACAATTGCAATGTCA+AGG - chr8.4:5306041-5306060 MS.gene57946:intron 40.0%
TATCCTCATATAGCATGAGC+TGG - chr8.4:5304051-5304070 MS.gene57946:intron 40.0%
TGACTGCTGAGAATGCAAAT+GGG - chr8.4:5304600-5304619 MS.gene57946:intron 40.0%
TGAGTTTGAACAAATCACGC+TGG - chr8.4:5303905-5303924 MS.gene57946:intron 40.0%
TGCAAACCAATAACTTCACG+TGG - chr8.4:5307202-5307221 MS.gene57946:intron 40.0%
TGCTTGGTATATAGTCTGCA+AGG + chr8.4:5303241-5303260 None:intergenic 40.0%
TGGAAGAGAAAAGGATCACA+TGG + chr8.4:5306484-5306503 None:intergenic 40.0%
TTGAATGGTTGCATACGAAG+TGG - chr8.4:5303392-5303411 MS.gene57946:intron 40.0%
TTGGATCCACGTGAAGTTAT+TGG + chr8.4:5307211-5307230 None:intergenic 40.0%
TTTGACACCAAACACGTTCA+TGG + chr8.4:5306460-5306479 None:intergenic 40.0%
! AAGAGATGGTGATGTTCAAG+AGG - chr8.4:5302279-5302298 MS.gene57946:CDS 40.0%
! ACAGCAGCTTTGGTCTTTAT+GGG - chr8.4:5305164-5305183 MS.gene57946:intron 40.0%
! ACCAAGCATCCCTAACTTTT+GGG - chr8.4:5303253-5303272 MS.gene57946:intron 40.0%
! ACTTGTAAATTTTCGGCTGG+AGG + chr8.4:5305002-5305021 None:intergenic 40.0%
! AGATCCATGTGCAGTTTGTT+TGG - chr8.4:5305899-5305918 MS.gene57946:intron 40.0%
! AGTCTGGTTGGGAATTCTTT+TGG - chr8.4:5302220-5302239 MS.gene57946:CDS 40.0%
! ATATTGGTTGACAGCAGCTT+TGG - chr8.4:5305154-5305173 MS.gene57946:intron 40.0%
! ATTTAAAGCAGCCAGATGGA+GGG + chr8.4:5303192-5303211 None:intergenic 40.0%
! CATGTGCAGTTTGTTTGGAA+AGG - chr8.4:5305904-5305923 MS.gene57946:intron 40.0%
! CTGCTTTAAATGGCCATACT+CGG - chr8.4:5303201-5303220 MS.gene57946:intron 40.0%
! CTTCAGCTATTGATTGCCAA+GGG - chr8.4:5304569-5304588 MS.gene57946:intron 40.0%
! CTTTTGGATGTTCTGCATCT+GGG - chr8.4:5302236-5302255 MS.gene57946:CDS 40.0%
! TACCAAGCATCCCTAACTTT+TGG - chr8.4:5303252-5303271 MS.gene57946:intron 40.0%
! TCTTCAGCTATTGATTGCCA+AGG - chr8.4:5304568-5304587 MS.gene57946:intron 40.0%
! TCTTTTGGATGTTCTGCATC+TGG - chr8.4:5302235-5302254 MS.gene57946:CDS 40.0%
! TGAAGAAGCACACATGGATA+TGG + chr8.4:5303673-5303692 None:intergenic 40.0%
! TTTTGGATGTTCTGCATCTG+GGG - chr8.4:5302237-5302256 MS.gene57946:CDS 40.0%
!! AAATGGTGTTGTCATGTCTG+TGG + chr8.4:5307182-5307201 None:intergenic 40.0%
!! CTTGCAAGGTATTCGACTTT+TGG - chr8.4:5302331-5302350 MS.gene57946:CDS 40.0%
!! TTGACAAGATGTTCTGGTTC+TGG - chr8.4:5307376-5307395 MS.gene57946:CDS 40.0%
!!! AGATGTTCTGGTTCTGGTTT+TGG - chr8.4:5307382-5307401 MS.gene57946:CDS 40.0%
AAAAACCCACTCAGACACCA+AGG + chr8.4:5304178-5304197 None:intergenic 45.0%
AAGCTTGCCAACATGGTCAT+TGG - chr8.4:5302991-5303010 MS.gene57946:intron 45.0%
ACTATACTGTGGCGACATAG+AGG + chr8.4:5307049-5307068 None:intergenic 45.0%
AGAGGACAAGCTATTGAACC+TGG + chr8.4:5307031-5307050 None:intergenic 45.0%
AGCTTGCCAACATGGTCATT+GGG - chr8.4:5302992-5303011 MS.gene57946:intron 45.0%
AGGACAAGCTATTGAACCTG+GGG + chr8.4:5307029-5307048 None:intergenic 45.0%
CAATCCGTTTCTACAGGAAG+TGG - chr8.4:5307460-5307479 MS.gene57946:CDS 45.0%
CATACGAAGTGGTGTTTGTG+AGG - chr8.4:5303403-5303422 MS.gene57946:intron 45.0%
CATGAACGAGCAACCATCAA+GGG + chr8.4:5304945-5304964 None:intergenic 45.0%
CTAGTTTCTGCAGCAAGAGA+TGG - chr8.4:5302265-5302284 MS.gene57946:CDS 45.0%
CTCGTTCATGGCATAGAAGT+TGG - chr8.4:5304955-5304974 MS.gene57946:intron 45.0%
CTGACTGCTGAGAATGCAAA+TGG - chr8.4:5304599-5304618 MS.gene57946:intron 45.0%
CTTCCAGCTCATGCTATATG+AGG + chr8.4:5304057-5304076 None:intergenic 45.0%
CTTGTGCAAGGAACCAATGA+AGG + chr8.4:5307328-5307347 None:intergenic 45.0%
GAGGACAAGCTATTGAACCT+GGG + chr8.4:5307030-5307049 None:intergenic 45.0%
GCAACTGCACACTTTCAACA+TGG + chr8.4:5303487-5303506 None:intergenic 45.0%
GCATCCACTTCCTGTAGAAA+CGG + chr8.4:5307467-5307486 None:intergenic 45.0%
GTGTATCTCTTGCTTGAGTC+TGG - chr8.4:5302496-5302515 MS.gene57946:CDS 45.0%
TCCATTATGCAGCATGTGGT+GGG - chr8.4:5304408-5304427 MS.gene57946:intron 45.0%
TCCCAAAAGTTAGGGATGCT+TGG + chr8.4:5303257-5303276 None:intergenic 45.0%
TCTCAGGAATTATCGTGGTC+AGG - chr8.4:5302531-5302550 MS.gene57946:CDS 45.0%
TCTTCGACTTCAACCTCTAC+TGG - chr8.4:5306526-5306545 MS.gene57946:intron 45.0%
TGAGCTGCAGAGTAATGCAA+AGG + chr8.4:5302370-5302389 None:intergenic 45.0%
TTCCTAACAGAGAATGTGGC+TGG - chr8.4:5305847-5305866 MS.gene57946:intron 45.0%
TTGAACAAATCACGCTGGCA+CGG - chr8.4:5303910-5303929 MS.gene57946:intron 45.0%
TTGATGCAAGCTTGCCAACA+TGG - chr8.4:5302984-5303003 MS.gene57946:intron 45.0%
TTGCATTACTCTGCAGCTCA+TGG - chr8.4:5302370-5302389 MS.gene57946:CDS 45.0%
TTTAGATTGGGGGGTGAGAT+TGG + chr8.4:5303998-5304017 None:intergenic 45.0%
! AGTTTGTTTGCCTTTGCGCT+GGG + chr8.4:5305504-5305523 None:intergenic 45.0%
! CAATGTCTGGTGGTGAGAAA+GGG + chr8.4:5306422-5306441 None:intergenic 45.0%
! CATTTAAAGCAGCCAGATGG+AGG + chr8.4:5303193-5303212 None:intergenic 45.0%
! CTCCATCTGGCTGCTTTAAA+TGG - chr8.4:5303191-5303210 MS.gene57946:intron 45.0%
! CTGCATATGGCAGCATTGAA+TGG - chr8.4:5303461-5303480 MS.gene57946:intron 45.0%
! GACAGCAGCTTTGGTCTTTA+TGG - chr8.4:5305163-5305182 MS.gene57946:intron 45.0%
! TGAAAGTGTGCAGTTGCTCT+TGG - chr8.4:5303490-5303509 MS.gene57946:intron 45.0%
! TTCGGCTGGAGGTTCTTTTA+AGG + chr8.4:5304991-5305010 None:intergenic 45.0%
! TTGAAGATGGACTCCCTTGA+TGG - chr8.4:5304929-5304948 MS.gene57946:intron 45.0%
!! ATGGCATAGAAGTTGGCTTG+AGG - chr8.4:5304962-5304981 MS.gene57946:intron 45.0%
!! TAGTTTGTTTGCCTTTGCGC+TGG + chr8.4:5305505-5305524 None:intergenic 45.0%
!! TTTGGTAGTGGTCTTGTGTC+TGG + chr8.4:5307088-5307107 None:intergenic 45.0%
AATGGCCAGGGTGTGTATGT+AGG + chr8.4:5303737-5303756 None:intergenic 50.0%
AGGCAAATCGCGGAGAATAG+TGG + chr8.4:5302793-5302812 None:intergenic 50.0%
CCAAACCTACATACACACCC+TGG - chr8.4:5303729-5303748 MS.gene57946:intron 50.0%
CCACTCCATTATGCAGCATG+TGG - chr8.4:5304404-5304423 MS.gene57946:intron 50.0%
CCAGGGTGTGTATGTAGGTT+TGG + chr8.4:5303732-5303751 None:intergenic 50.0%
CCATGAACGAGCAACCATCA+AGG + chr8.4:5304946-5304965 None:intergenic 50.0%
CTAACAGAGAATGTGGCTGG+AGG - chr8.4:5305850-5305869 MS.gene57946:intron 50.0%
CTCCAGCCACATTCTCTGTT+AGG + chr8.4:5305852-5305871 None:intergenic 50.0%
CTCCATTATGCAGCATGTGG+TGG - chr8.4:5304407-5304426 MS.gene57946:intron 50.0%
GAGATTGGCAGAATCACGGA+GGG + chr8.4:5303983-5304002 None:intergenic 50.0%
GGACAAGCTATTGAACCTGG+GGG + chr8.4:5307028-5307047 None:intergenic 50.0%
GGGTGAGATTGGCAGAATCA+CGG + chr8.4:5303987-5304006 None:intergenic 50.0%
GTCGAATACCTTGCAAGACG+TGG + chr8.4:5302328-5302347 None:intergenic 50.0%
GTGCAGTTGCTCTTGGACTT+GGG - chr8.4:5303497-5303516 MS.gene57946:intron 50.0%
GTTTGTTTGCCTTTGCGCTG+GGG + chr8.4:5305503-5305522 None:intergenic 50.0%
TCCATCCTCTACGGTAACCT+CGG + chr8.4:5303534-5303553 None:intergenic 50.0%
TCCCACCACATGCTGCATAA+TGG + chr8.4:5304412-5304431 None:intergenic 50.0%
TGAGATTGGCAGAATCACGG+AGG + chr8.4:5303984-5304003 None:intergenic 50.0%
TGTGCAGTTGCTCTTGGACT+TGG - chr8.4:5303496-5303515 MS.gene57946:intron 50.0%
TTAGCCGAATGCACCGAGTA+TGG + chr8.4:5303217-5303236 None:intergenic 50.0%
TTGCCAACATGGTCATTGGG+AGG - chr8.4:5302995-5303014 MS.gene57946:intron 50.0%
! CCACATGCTGCATAATGGAG+TGG + chr8.4:5304407-5304426 None:intergenic 50.0%
! CCTTGATGGTTGCTCGTTCA+TGG - chr8.4:5304943-5304962 MS.gene57946:intron 50.0%
! GCAATGTCTGGTGGTGAGAA+AGG + chr8.4:5306423-5306442 None:intergenic 50.0%
! GGCCATTTAAAGCAGCCAGA+TGG + chr8.4:5303196-5303215 None:intergenic 50.0%
! TGGTGATGTTCAAGAGGCGA+AGG - chr8.4:5302285-5302304 MS.gene57946:CDS 50.0%
!! ATGGCCATACTCGGTGCATT+CGG - chr8.4:5303210-5303229 MS.gene57946:intron 50.0%
ACAAGAACACATGGTCCCCC+AGG - chr8.4:5307010-5307029 MS.gene57946:intron 55.0%
AGCAGTCAGACTAGCACCCT+TGG + chr8.4:5304588-5304607 None:intergenic 55.0%
AGGCATCACTGCGCTGCATA+TGG - chr8.4:5303448-5303467 MS.gene57946:intron 55.0%
ATACCGGCACTGTAGCGTAG+CGG - chr8.4:5302755-5302774 MS.gene57946:intron 55.0%
ATTCCGCTACGCTACAGTGC+CGG + chr8.4:5302761-5302780 None:intergenic 55.0%
CAAGTCAACTAGCAGCCAGC+AGG - chr8.4:5305283-5305302 MS.gene57946:CDS 55.0%
CCAACATGGTCATTGGGAGG+TGG - chr8.4:5302998-5303017 MS.gene57946:intron 55.0%
CCACCTCCCAATGACCATGT+TGG + chr8.4:5303001-5303020 None:intergenic 55.0%
TCCGAGGTTACCGTAGAGGA+TGG - chr8.4:5303530-5303549 MS.gene57946:intron 55.0%
TGTGTCCGAGGTTACCGTAG+AGG - chr8.4:5303526-5303545 MS.gene57946:intron 55.0%
!! CACAGCCTTGGTGTCTGAGT+GGG - chr8.4:5304170-5304189 MS.gene57946:intron 55.0%
!! CTACGCTACAGTGCCGGTAT+AGG + chr8.4:5302755-5302774 None:intergenic 55.0%
CTTGGGAGCTTCTGTGTCCG+AGG - chr8.4:5303514-5303533 MS.gene57946:intron 60.0%
GGAGGGCAGTACCTCCATTC+AGG + chr8.4:5303175-5303194 None:intergenic 60.0%
GTCAACTAGCAGCCAGCAGG+TGG - chr8.4:5305286-5305305 MS.gene57946:CDS 60.0%
TGCAGCTCATGGCCACCATG+AGG - chr8.4:5302381-5302400 MS.gene57946:CDS 60.0%
TGGAGAGATCCCCAGCGCAA+AGG - chr8.4:5305491-5305510 MS.gene57946:intron 60.0%
!! GCACAGCCTTGGTGTCTGAG+TGG - chr8.4:5304169-5304188 MS.gene57946:intron 60.0%
!! TGGAGGTACTGCCCTCCATC+TGG - chr8.4:5303178-5303197 MS.gene57946:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 5302208 5307489 5302208 ID=MS.gene57946
chr8.4 mRNA 5302208 5307489 5302208 ID=MS.gene57946.t1;Parent=MS.gene57946
chr8.4 exon 5307295 5307489 5307295 ID=MS.gene57946.t1.exon1;Parent=MS.gene57946.t1
chr8.4 CDS 5307295 5307489 5307295 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5307145 5307204 5307145 ID=MS.gene57946.t1.exon2;Parent=MS.gene57946.t1
chr8.4 CDS 5307145 5307204 5307145 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5306648 5306719 5306648 ID=MS.gene57946.t1.exon3;Parent=MS.gene57946.t1
chr8.4 CDS 5306648 5306719 5306648 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5306373 5306542 5306373 ID=MS.gene57946.t1.exon4;Parent=MS.gene57946.t1
chr8.4 CDS 5306373 5306542 5306373 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5306125 5306285 5306125 ID=MS.gene57946.t1.exon5;Parent=MS.gene57946.t1
chr8.4 CDS 5306125 5306285 5306125 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5305249 5305307 5305249 ID=MS.gene57946.t1.exon6;Parent=MS.gene57946.t1
chr8.4 CDS 5305249 5305307 5305249 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5305076 5305122 5305076 ID=MS.gene57946.t1.exon7;Parent=MS.gene57946.t1
chr8.4 CDS 5305076 5305122 5305076 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5304622 5304762 5304622 ID=MS.gene57946.t1.exon8;Parent=MS.gene57946.t1
chr8.4 CDS 5304622 5304762 5304622 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5303749 5303840 5303749 ID=MS.gene57946.t1.exon9;Parent=MS.gene57946.t1
chr8.4 CDS 5303749 5303840 5303749 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
chr8.4 exon 5302208 5302749 5302208 ID=MS.gene57946.t1.exon10;Parent=MS.gene57946.t1
chr8.4 CDS 5302208 5302749 5302208 ID=cds.MS.gene57946.t1;Parent=MS.gene57946.t1
Gene Sequence

>MS.gene57946

ATGAAATTTCTAAGTCTGGTTGGGAATTCTTTTGGATGTTCTGCATCTGGGGAGAGACTAGTTTCTGCAGCAAGAGATGGTGATGTTCAAGAGGCGAAGGCCTTGTTAGAATATAATCCACGTCTTGCAAGGTATTCGACTTTTGGAGTTCGTAATTCTCCTTTGCATTACTCTGCAGCTCATGGCCACCATGAGGTATATACTTACAATTCAATGCACCTCATTTTCTTGTATCGTGTTTTTCGCAATTAGTCTTTGACAAATCTATTGTGTTTTGCATTGTAGATAGTGTATCTCTTGCTTGAGTCTGGTGTTGATATTAATCTCAGGAATTATCGTGGTCAGGTAATCCAATATTATTTTGTTTCAATGTTTTTTTCCTTAATTTAGTTGACTATATCTATTGTCGCATAAATGCGAAGTTGTAGCACAATCTAGCATAGTGATGTCAAATAGAAGCTATAGCACAACAGATTGATGGAGTTTGGACAAACTGCTATTGTTCTGCGATACGCAATTTAGTACAAATTGTTTTCAAATTGTGCCTATACCGGCACTGTAGCGTAGCGGAATTCAAACAAACCACTATTCTCCGCGATTTGCCTTTGAAAACATTGATCTAGCACACAATCTTCTGCTGATTGTCTAAAACTGTAAATTTTAATTTTATGCTTCAAACTTTTGTTGTTTTGGGTATGAACAGCGCCATGTTTCTTTCGTTTACAGAGGGAAACTTCTTTTACTTACTTTATACTTTATATCATCGACAGACTGCATTGATGCAAGCTTGCCAACATGGTCATTGGGAGGTGGTTCAGACCCTGATTATTTTTAAAGCCAATGTAAGATCATTACTGCAAACCGATTTTGTGCTATTTTATTAACAATGTGATAAATTATTAGTAGAATGAGTTGTATAAAATATTGCTTGTTAAATTGGACTGCAGATTCACAAAACAGATTACCTGAATGGAGGTACTGCCCTCCATCTGGCTGCTTTAAATGGCCATACTCGGTGCATTCGGCTAATCCTTGCAGACTATATACCAAGCATCCCTAACTTTTGGGATATATTACAGACAGGTGATCAGACATCAATCTCAGAATTTGATCAAAGGTACTCTCACTCAGAAGTTCTATTGTAGTCATCCATTATAAACTGTTGCAGTAAGTTAATTCTGTCTTTGAATGGTTGCATACGAAGTGGTGTTTGTGAGGTAATTAACAAAACTTCTGATGGAGGCATCACTGCGCTGCATATGGCAGCATTGAATGGCCATGTTGAAAGTGTGCAGTTGCTCTTGGACTTGGGAGCTTCTGTGTCCGAGGTTACCGTAGAGGATGGAACTACTATTGATTTAATTGGTAATTATATATCATCTCCTAGTTCATGTGATTAAGTGATGTCAGATACTTGCTTTGGAAAGTCGGCATTTATATTCCTACTATCTCTCGATTGTTTCCATATCCATGTGTGCTTCTTCATTTTTCTTTTGGAAACTTGCATAATTGGTATTGTGACCAAACCTACATACACACCCTGGCCATTTCTCATCAATATTGTTCAACATTTGGTTTGTAGAGAAATTTTAAGAAGTGCTTAGTTGAACTTGATAACTAACTTACTTTACAATGGCTTGGCACAATAAAATTTGTAGTGGTAGAAATTCAACTGCAACTCATGTTTGGATTGGCAGTGAGTTTGAACAAATCACGCTGGCACGGTGATTTTATCGAAGCTTCAAAGTGTACGTTTGTTAAAATCATGGTGGCCCTCCGTGATTCTGCCAATCTCACCCCCCAATCTAAACATTTACTATATTCTATACCAAAACTGTTTATTTATCCTCATATAGCATGAGCTGGAAGAACAATGAACTAATACTAAAAACCTTTCCATCATATTCCATTATCTACATTCTACACATTGCACTGTTTTCCTCTTGTCTAATGTTTACTAAGCACAGCCTTGGTGTCTGAGTGGGTTTTTCTCCCTCTTTCACCTACTTCTAAATCTTAGCAGTATAGCTCTCAGTAAACATTTATAGAATTCAATTAGGAGAGAGATTAGAACTAATTTTTTTTCCCCTGTACTACATAAGAGAAAATGCTTCATGTTTAAAGCGGACCCCTAAGTTAACTAGTTTGATCTCCACTAACTGCTATCTATTTTTATTTACAGGTTCTGGGAGCACTCCACTCCATTATGCAGCATGTGGTGGGAATGCACAATGTTGTCAAGTGTGTTTCTATTTTCCTTTAGAAGACTGTTTCATTTTAGGATAAAAATATAATTTTGAAACTGAAAATATATTGCTGCTCTTCATATTGAATTTACATTTATTTTTTATTGTGTATTATCTTCAGCTATTGATTGCCAAGGGTGCTAGTCTGACTGCTGAGAATGCAAATGGGTATGTATAAATACATATTATTTGTTAAGTTTGATGTGTGATAAACTTACCTTATATACAGCTTGGCATGTTAAACATATAAATATTATGATCTATTTAGATCATAGGAAATTTAATAACCTTTACCCCTTGTTTTTAACTTACTACGAGATTCACTATCTATCGTATTGGAACTATGTACAATTTTATAAGTTTATGTATAGAACTTTGTTGTACTTGCATGAAGCAAACATAGATTTGTATTGTCTCAAGCACTATGTATCTTAAATGGATTTCAAATTGTTTTGTTTACTCTATTGATTTTGTTTTGAAGATGGACTCCCTTGATGGTTGCTCGTTCATGGCATAGAAGTTGGCTTGAGGACACCTTAAAAGAACCTCCAGCCGAAAATTTACAAGTTCTTCCTTCGCCATATATATCTCTTCCACTTATGAGCATTGTCAAAATTGCTAGGTATGTGTCTTTCAGAGCCATCCTCTCAAAATTCAACCAATTTCTTCCTAACATTTAATTAATAACCTTTCAAGATTGATATTGGTTGACAGCAGCTTTGGTCTTTATGGGAAAAACCATGTGAAAAATTGTTTTAATAGAAATTAGCATCATTTGATTTTAACAAGGACATTTCTTTCCATGAATTTATTGACTCTGCTCTGATTTCAAGTCAACTAGCAGCCAGCAGGTGGATTTAACTGATTATACTTTGAATGCGGTGACAAACATGATTATCAAAGAGTTCCGAATAAAATTCTACGATACTAGGCAAAATTTACTCAAATTTATATTTATTTGGTTGTTGTTCATGTGATTATGTGCATATTTGGTTGAGTTAGAGATCTTCAATATAGGTTTTGCCAATACTTTGGTTTGGAGAGATCCCCAGCGCAAAGGCAAACAAACTATTTGTTGCTAATTAGATGAGTTTAGTCATTTAAGGTTCATTAATTACATATCTAATTTCAGTGTCATCAGCTTCATTGAAAAGTGCTTTGATCTTTTCATTTCATTTATAGCTTAACCAAATAATGCTATATTTGTTCAAATCAACCTATTAGATTATGAAGTTTTTAAGTAAATGAAAGCGAACACACTTCTAATATTTTAATGCACTTATGTTGACAGTAATGAGTGAACTTAGTACATAATTTACTCTGCTTCAAATTTTATGTATTCCAAAGGTTACTATTATTGATCTTTCCCTCTTCTTGCGTATGTTGTTATCAATTCCTAACAGAGAATGTGGCTGGAGGACAAGTGAATTAACACCTATATGCTTAGATCCATGTGCAGTTTGTTTGGAAAGGAAATGTATGGTCGCTGTAGAAGGTATAACACTCTTTTAATTTACACTCAATGCTGATATAGATGGTTTTACATGGAAAAATGATCTGGACTTTTAAGATGCTTAAACTTTATATGTGCAACAATTGCAATGTCAAGGAAGCTTGGTCTCATTACCAGAAAATACATTCAAAGTAGATATTCTCAAAAATCTAAATGTTCTATTTACAATATCTAGTCATTTCATTTTGACTGATATATTTATTTACATATATTAAGTAATTGGTCAGGCTTGGATATGTTTTCTACAAATGGTATGATTCTGTCAAATAATAAGAGCAAATAATTAGAAAGTAATGGTGACAGCACATGTTCACACACCAAAAACAAATGGAAAAAACATAACTATGCTTCTTTGTTGGTAATGACAACCTTTGAATCAATGGTAAAGGAATAGAGCCATAGAGAAACGTATTTTGCAAGTGTTTCTATATGGAGTTATTGCACGGTCATATCCCTTTCTCACCACCAGACATTGCTCAGTTTTTATTTTCCATGAACGTGTTTGGTGTCAAATCCATGTGATCCTTTTCTCTTCCATTTTATATTTTGATGTCTTTTCTCTTCGACTTCAACCTCTACTGGTTTATAATTTTACACCCATATTTTGTCATCTACCTCTTTGTATTTTTATTGGCAAAATTATGCCACACATTAGAACCCAAAATACTCTAGTAGAAATTGAAGATAAAAACCATCAATTCAATGCATGTTTGGATTGACGCTAAGTTTGATGAATTCACCATGACAACGTGATTTTGTTGAAACTAAGTGCAGCTTAGTTACAATTGTCCTATGCACAATTTTGTCATACCTTGTCAATTCAAACACTAAACCAATATTGTTAAGGTGACACCTCATAGACCTATGACATTAGAAATTATTGTTATTGGCTTAAAAAGTGTTGGTTCATAGATTTCAGTAATTGACACAAATTACTGCTATTTATGTGAGACTAATGCATATGATAAATTGATAATGCAGGTTGTGATCATGAATTCTGTACACAGTGTGCCTTATACCTATGTTCCACAAACTCAACTTCAACAAGAACACATGGTCCCCCAGGTTCAATAGCTTGTCCTCTATGTCGCCACAGTATAGTCTCATTTGTTAAGCTTCCAGACACAAGACCACTACCAAAAGTAATGCAAAGGACAACAACAAACTTGTCCTTAACATTTTGCACGTGTTCAAGTGAAGTTCTTGGTGATTCCACAGACATGACAACACCATTTTGCAAACCAATAACTTCACGTGGATCCAAATTTTCTTCCCCTTCAAGAAGATTTCGTTCCTTGAGTTGTCAATTCTCTTCATTCAGACTCAACTCTAGTCTTTGTTTAGGAGCTGATGTTACTCCTTCATTGGTTCCTTGCACAAGTGCTAATAGAAGAAACTTGAGGAACCATTTGACAAGATGTTCTGGTTCTGGTTTTGGAAGATCAAGTTCTCAAACTGAGAGAAGGAAATCATCTTGGTTTTGTTCCTTCAATCAATCCGTTTCTACAGGAAGTGGATGCTAA
Protein sequence

>MS.gene57946.t1

MKFLSLVGNSFGCSASGERLVSAARDGDVQEAKALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVYLLLESGVDINLRNYRGQTALMQACQHGHWEVVQTLIIFKANIHKTDYLNGGTALHLAALNGHTRCIRLILADYIPSIPNFWDILQTGDQTSISEFDQSGVCEVINKTSDGGITALHMAALNGHVESVQLLLDLGASVSEVTVEDGTTIDLIGSGSTPLHYAACGGNAQCCQLLIAKGASLTAENANGWTPLMVARSWHRSWLEDTLKEPPAENLQVLPSPYISLPLMSIVKIARECGWRTSELTPICLDPCAVCLERKCMVAVEGCDHEFCTQCALYLCSTNSTSTRTHGPPGSIACPLCRHSIVSFVKLPDTRPLPKVMQRTTTNLSLTFCTCSSEVLGDSTDMTTPFCKPITSRGSKFSSPSRRFRSLSCQFSSFRLNSSLCLGADVTPSLVPCTSANRRNLRNHLTRCSGSGFGRSSSQTERRKSSWFCSFNQSVSTGSGC