Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072564.t1 | XP_013450442.1 | 96.8 | 158 | 2 | 2 | 1 | 157 | 1 | 156 | 6.90E-77 | 296.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072564.t1 | B7FGJ8 | 96.8 | 158 | 2 | 2 | 1 | 157 | 1 | 156 | 4.9e-77 | 296.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene072564 | 0.838607 | 2.60E-57 | -1.69E-46 |
| MS.gene049178 | MS.gene072564 | 0.820285 | 7.44E-53 | -1.69E-46 |
| MS.gene04917 | MS.gene072564 | 0.83155 | 1.57E-55 | -1.69E-46 |
| MS.gene049322 | MS.gene072564 | 0.809031 | 2.31E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene072564 | 0.831598 | 1.52E-55 | -1.69E-46 |
| MS.gene049382 | MS.gene072564 | 0.821531 | 3.84E-53 | -1.69E-46 |
| MS.gene049685 | MS.gene072564 | 0.800205 | 1.61E-48 | -1.69E-46 |
| MS.gene049756 | MS.gene072564 | 0.818428 | 1.97E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene072564 | 0.82241 | 2.41E-53 | -1.69E-46 |
| MS.gene04984 | MS.gene072564 | 0.847327 | 1.24E-59 | -1.69E-46 |
| MS.gene04991 | MS.gene072564 | -0.801746 | 7.78E-49 | -1.69E-46 |
| MS.gene050114 | MS.gene072564 | 0.80189 | 7.27E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene072564 | 0.829394 | 5.27E-55 | -1.69E-46 |
| MS.gene050227 | MS.gene072564 | 0.808884 | 2.49E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene072564 | 0.809403 | 1.92E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene072564 | 0.80977 | 1.61E-50 | -1.69E-46 |
| MS.gene050361 | MS.gene072564 | 0.804853 | 1.77E-49 | -1.69E-46 |
| MS.gene050527 | MS.gene072564 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene072564 | -0.854699 | 1.03E-61 | -1.69E-46 |
| MS.gene050629 | MS.gene072564 | -0.838045 | 3.63E-57 | -1.69E-46 |
| MS.gene050735 | MS.gene072564 | 0.820442 | 6.85E-53 | -1.69E-46 |
| MS.gene050762 | MS.gene072564 | 0.982075 | 3.61E-154 | -1.69E-46 |
| MS.gene050763 | MS.gene072564 | 0.980939 | 2.16E-151 | -1.69E-46 |
| MS.gene050826 | MS.gene072564 | 0.832626 | 8.49E-56 | -1.69E-46 |
| MS.gene050987 | MS.gene072564 | 0.801274 | 9.73E-49 | -1.69E-46 |
| MS.gene051535 | MS.gene072564 | 0.824293 | 8.73E-54 | -1.69E-46 |
| MS.gene051631 | MS.gene072564 | -0.834407 | 3.05E-56 | -1.69E-46 |
| MS.gene052115 | MS.gene072564 | 0.808081 | 3.69E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene072564 | 0.831955 | 1.24E-55 | -1.69E-46 |
| MS.gene052856 | MS.gene072564 | 0.814647 | 1.39E-51 | -1.69E-46 |
| MS.gene052970 | MS.gene072564 | -0.815599 | 8.51E-52 | -1.69E-46 |
| MS.gene053811 | MS.gene072564 | 0.801943 | 7.09E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene072564 | 0.946449 | 4.52E-105 | -1.69E-46 |
| MS.gene054930 | MS.gene072564 | 0.805942 | 1.05E-49 | -1.69E-46 |
| MS.gene055207 | MS.gene072564 | 0.807815 | 4.20E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene072564 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene055413 | MS.gene072564 | 0.826467 | 2.67E-54 | -1.69E-46 |
| MS.gene055441 | MS.gene072564 | 0.806146 | 9.48E-50 | -1.69E-46 |
| MS.gene055816 | MS.gene072564 | -0.80446 | 2.14E-49 | -1.69E-46 |
| MS.gene056400 | MS.gene072564 | 0.82378 | 1.15E-53 | -1.69E-46 |
| MS.gene056464 | MS.gene072564 | -0.81251 | 4.09E-51 | -1.69E-46 |
| MS.gene056480 | MS.gene072564 | 0.804634 | 1.97E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene072564 | 0.808547 | 2.94E-50 | -1.69E-46 |
| MS.gene056849 | MS.gene072564 | 0.801109 | 1.05E-48 | -1.69E-46 |
| MS.gene057451 | MS.gene072564 | -0.823323 | 1.47E-53 | -1.69E-46 |
| MS.gene057452 | MS.gene072564 | -0.825318 | 5.00E-54 | -1.69E-46 |
| MS.gene057665 | MS.gene072564 | 0.805951 | 1.04E-49 | -1.69E-46 |
| MS.gene057929 | MS.gene072564 | 0.831278 | 1.83E-55 | -1.69E-46 |
| MS.gene058182 | MS.gene072564 | 0.842184 | 3.02E-58 | -1.69E-46 |
| MS.gene058638 | MS.gene072564 | 0.846628 | 1.93E-59 | -1.69E-46 |
| MS.gene058639 | MS.gene072564 | 0.858634 | 7.20E-63 | -1.69E-46 |
| MS.gene058758 | MS.gene072564 | 0.831145 | 1.97E-55 | -1.69E-46 |
| MS.gene059327 | MS.gene072564 | 0.804581 | 2.02E-49 | -1.69E-46 |
| MS.gene059713 | MS.gene072564 | 0.832989 | 6.90E-56 | -1.69E-46 |
| MS.gene059719 | MS.gene072564 | 0.800468 | 1.42E-48 | -1.69E-46 |
| MS.gene059725 | MS.gene072564 | 0.802039 | 6.78E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene072564 | 0.892474 | 1.54E-74 | -1.69E-46 |
| MS.gene060296 | MS.gene072564 | 0.833195 | 6.13E-56 | -1.69E-46 |
| MS.gene060297 | MS.gene072564 | 0.806561 | 7.75E-50 | -1.69E-46 |
| MS.gene060400 | MS.gene072564 | 0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene060480 | MS.gene072564 | 0.818754 | 1.66E-52 | -1.69E-46 |
| MS.gene060495 | MS.gene072564 | 0.801745 | 7.79E-49 | -1.69E-46 |
| MS.gene060563 | MS.gene072564 | 0.809628 | 1.72E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene072564 | 0.816351 | 5.78E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene072564 | 0.831646 | 1.48E-55 | -1.69E-46 |
| MS.gene060803 | MS.gene072564 | 0.821913 | 3.14E-53 | -1.69E-46 |
| MS.gene060869 | MS.gene072564 | 0.84144 | 4.75E-58 | -1.69E-46 |
| MS.gene060870 | MS.gene072564 | 0.827681 | 1.37E-54 | -1.69E-46 |
| MS.gene060940 | MS.gene072564 | 0.806933 | 6.47E-50 | -1.69E-46 |
| MS.gene060943 | MS.gene072564 | 0.807305 | 5.39E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene072564 | 0.808033 | 3.78E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072564.t1 | MTR_7g111900 | 96.835 | 158 | 2 | 2 | 1 | 157 | 1 | 156 | 1.56e-106 | 301 |
| MS.gene072564.t1 | MTR_7g103200 | 85.065 | 154 | 21 | 1 | 1 | 154 | 1 | 152 | 4.69e-92 | 264 |
| MS.gene072564.t1 | MTR_1g084200 | 71.233 | 146 | 42 | 0 | 1 | 146 | 1 | 146 | 4.93e-73 | 216 |
| MS.gene072564.t1 | MTR_2g019480 | 49.254 | 134 | 68 | 0 | 10 | 143 | 14 | 147 | 9.26e-49 | 155 |
| MS.gene072564.t1 | MTR_2g046150 | 47.917 | 144 | 74 | 1 | 2 | 145 | 6 | 148 | 5.70e-40 | 132 |
| MS.gene072564.t1 | MTR_4g122930 | 47.059 | 153 | 77 | 2 | 1 | 151 | 1 | 151 | 6.37e-39 | 129 |
| MS.gene072564.t1 | MTR_2g091210 | 45.528 | 123 | 67 | 0 | 17 | 139 | 12 | 134 | 8.12e-34 | 116 |
| MS.gene072564.t1 | MTR_2g091215 | 53.636 | 110 | 51 | 0 | 28 | 137 | 23 | 132 | 1.01e-32 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072564.t1 | AT1G56580 | 66.250 | 160 | 48 | 3 | 1 | 154 | 1 | 160 | 1.35e-68 | 205 |
| MS.gene072564.t1 | AT1G09310 | 65.868 | 167 | 43 | 3 | 1 | 154 | 1 | 166 | 3.69e-68 | 204 |
| MS.gene072564.t1 | AT5G46230 | 47.692 | 130 | 68 | 0 | 12 | 141 | 14 | 143 | 3.25e-46 | 147 |
| MS.gene072564.t1 | AT4G24130 | 45.185 | 135 | 74 | 0 | 9 | 143 | 13 | 147 | 4.06e-39 | 130 |
| MS.gene072564.t1 | AT1G30020 | 43.571 | 140 | 77 | 1 | 13 | 150 | 15 | 154 | 8.88e-37 | 124 |
| MS.gene072564.t1 | AT5G49600 | 46.897 | 145 | 67 | 3 | 3 | 138 | 1 | 144 | 4.37e-35 | 120 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCTTTGAGAAGCTCTT+TGG | 0.291146 | 7.3:-4504454 | None:intergenic |
| TTCAATGGTAAAAGACCATT+TGG | 0.295506 | 7.3:-4504483 | None:intergenic |
| GTCACTGCTTACGTTGAAAA+TGG | 0.305607 | 7.3:+4504621 | MS.gene072564:CDS |
| ATCACATTCAAAACACCTTC+TGG | 0.305889 | 7.3:+4504738 | MS.gene072564:CDS |
| AACCCAAATCAAAAGCTCTT+TGG | 0.308533 | 7.3:-4504673 | None:intergenic |
| TCAATTTCAAAAGCAGAAAC+AGG | 0.316797 | 7.3:-4504777 | None:intergenic |
| TCCATGGTAAACCTCGGATT+TGG | 0.319079 | 7.3:-4504409 | None:intergenic |
| GTTGGTTACAACAGAGAAAC+AGG | 0.372734 | 7.3:+4504519 | MS.gene072564:CDS |
| ACACACAAATTCGAAAAGAT+TGG | 0.384478 | 7.3:+4504573 | MS.gene072564:CDS |
| ATCAAGTGGTGCTACTGTTA+AGG | 0.417389 | 7.3:+4504815 | MS.gene072564:CDS |
| ACTTCGGTAGCATAGGAAAC+AGG | 0.457776 | 7.3:-4504600 | None:intergenic |
| TATATTGATGATCCACCAAC+TGG | 0.471274 | 7.3:+4504711 | MS.gene072564:CDS |
| CACACAAATTCGAAAAGATT+GGG | 0.476488 | 7.3:+4504574 | MS.gene072564:CDS |
| TTGAAAGACATCACTGAAGT+TGG | 0.485594 | 7.3:+4504501 | MS.gene072564:CDS |
| AAAGAAATCTCTCTCCCAAA+TGG | 0.487795 | 7.3:+4504468 | MS.gene072564:CDS |
| TCAATGGTAAAAGACCATTT+GGG | 0.506164 | 7.3:-4504482 | None:intergenic |
| CCATGGAGACGAAATGTGTC+AGG | 0.515719 | 7.3:+4504425 | MS.gene072564:CDS |
| ACTTCAGTGATGTCTTTCAA+TGG | 0.520310 | 7.3:-4504498 | None:intergenic |
| CCTGACACATTTCGTCTCCA+TGG | 0.557991 | 7.3:-4504425 | None:intergenic |
| GAAGAGAAAGAGAAATCAAG+TGG | 0.560001 | 7.3:+4504801 | MS.gene072564:CDS |
| TGTTAAGGAAGCTGCTAACA+AGG | 0.566122 | 7.3:+4504830 | MS.gene072564:CDS |
| TTCGTCTCCATGGTAAACCT+CGG | 0.581474 | 7.3:-4504415 | None:intergenic |
| TTCAACGTAAGCAGTGACTT+CGG | 0.602234 | 7.3:-4504616 | None:intergenic |
| CAATTTCAAAAGCAGAAACA+GGG | 0.604117 | 7.3:-4504776 | None:intergenic |
| AGCAGTGACTTCGGTAGCAT+AGG | 0.607903 | 7.3:-4504607 | None:intergenic |
| TCCAAATCCGAGGTTTACCA+TGG | 0.611352 | 7.3:+4504408 | MS.gene072564:CDS |
| CAACCCCAAAACGATGTCGT+TGG | 0.618459 | 7.3:+4504368 | None:intergenic |
| GGTAAGATCAAGAAACTGAA+CGG | 0.640251 | 7.3:+4504642 | MS.gene072564:CDS |
| AGAAATCAAATCCAAATCCG+AGG | 0.652928 | 7.3:+4504398 | MS.gene072564:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACACACAAATTCGAAAAGAT+TGG | + | chr7.3:4504573-4504592 | MS.gene072564:CDS | 30.0% | |
| CAATTTCAAAAGCAGAAACA+GGG | - | chr7.3:4504779-4504798 | None:intergenic | 30.0% | |
| CACACAAATTCGAAAAGATT+GGG | + | chr7.3:4504574-4504593 | MS.gene072564:CDS | 30.0% | |
| TCAATGGTAAAAGACCATTT+GGG | - | chr7.3:4504485-4504504 | None:intergenic | 30.0% | |
| TCAATTTCAAAAGCAGAAAC+AGG | - | chr7.3:4504780-4504799 | None:intergenic | 30.0% | |
| TTCAATGGTAAAAGACCATT+TGG | - | chr7.3:4504486-4504505 | None:intergenic | 30.0% | |
| ! | AAACTTCTAAAAAGTCCAGA+AGG | - | chr7.3:4504756-4504775 | None:intergenic | 30.0% |
| ! | TTGAATGTGATTTTACCAGT+TGG | - | chr7.3:4504729-4504748 | None:intergenic | 30.0% |
| !! | AAACCAAAGAGCTTTTGATT+TGG | + | chr7.3:4504670-4504689 | MS.gene072564:CDS | 30.0% |
| !!! | AACCAAAGAGCTTTTGATTT+GGG | + | chr7.3:4504671-4504690 | MS.gene072564:CDS | 30.0% |
| AAAGAAATCTCTCTCCCAAA+TGG | + | chr7.3:4504468-4504487 | MS.gene072564:CDS | 35.0% | |
| AACCCAAATCAAAAGCTCTT+TGG | - | chr7.3:4504676-4504695 | None:intergenic | 35.0% | |
| ACTTCAGTGATGTCTTTCAA+TGG | - | chr7.3:4504501-4504520 | None:intergenic | 35.0% | |
| AGAAATCAAATCCAAATCCG+AGG | + | chr7.3:4504398-4504417 | MS.gene072564:CDS | 35.0% | |
| ATCACATTCAAAACACCTTC+TGG | + | chr7.3:4504738-4504757 | MS.gene072564:CDS | 35.0% | |
| GAAGAGAAAGAGAAATCAAG+TGG | + | chr7.3:4504801-4504820 | MS.gene072564:CDS | 35.0% | |
| GGTAAGATCAAGAAACTGAA+CGG | + | chr7.3:4504642-4504661 | MS.gene072564:CDS | 35.0% | |
| TTGAAAGACATCACTGAAGT+TGG | + | chr7.3:4504501-4504520 | MS.gene072564:CDS | 35.0% | |
| ! | AATGTGATTTTACCAGTTGG+TGG | - | chr7.3:4504726-4504745 | None:intergenic | 35.0% |
| ! | GATTTCTTTGAGAAGCTCTT+TGG | - | chr7.3:4504457-4504476 | None:intergenic | 35.0% |
| ! | TATATTGATGATCCACCAAC+TGG | + | chr7.3:4504711-4504730 | MS.gene072564:CDS | 35.0% |
| !!! | ACAGAGAAACAGGTTTTGTT+TGG | + | chr7.3:4504529-4504548 | MS.gene072564:CDS | 35.0% |
| GTCACTGCTTACGTTGAAAA+TGG | + | chr7.3:4504621-4504640 | MS.gene072564:CDS | 40.0% | |
| GTTGGTTACAACAGAGAAAC+AGG | + | chr7.3:4504519-4504538 | MS.gene072564:CDS | 40.0% | |
| TTCAACGTAAGCAGTGACTT+CGG | - | chr7.3:4504619-4504638 | None:intergenic | 40.0% | |
| !! | ATCAAGTGGTGCTACTGTTA+AGG | + | chr7.3:4504815-4504834 | MS.gene072564:CDS | 40.0% |
| ACTTCGGTAGCATAGGAAAC+AGG | - | chr7.3:4504603-4504622 | None:intergenic | 45.0% | |
| TCCAAATCCGAGGTTTACCA+TGG | + | chr7.3:4504408-4504427 | MS.gene072564:CDS | 45.0% | |
| TCCATGGTAAACCTCGGATT+TGG | - | chr7.3:4504412-4504431 | None:intergenic | 45.0% | |
| TTCGTCTCCATGGTAAACCT+CGG | - | chr7.3:4504418-4504437 | None:intergenic | 45.0% | |
| AGCAGTGACTTCGGTAGCAT+AGG | - | chr7.3:4504610-4504629 | None:intergenic | 50.0% | |
| CCATGGAGACGAAATGTGTC+AGG | + | chr7.3:4504425-4504444 | MS.gene072564:CDS | 50.0% | |
| CCTGACACATTTCGTCTCCA+TGG | - | chr7.3:4504428-4504447 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 4504381 | 4504851 | 4504381 | ID=MS.gene072564 |
| chr7.3 | mRNA | 4504381 | 4504851 | 4504381 | ID=MS.gene072564.t1;Parent=MS.gene072564 |
| chr7.3 | exon | 4504381 | 4504851 | 4504381 | ID=MS.gene072564.t1.exon1;Parent=MS.gene072564.t1 |
| chr7.3 | CDS | 4504381 | 4504851 | 4504381 | ID=cds.MS.gene072564.t1;Parent=MS.gene072564.t1 |
| Gene Sequence |
| Protein sequence |
