AlfalfaGEDB | MS.gene063544

M. sativa cultivar XinJiangDaYe / MS.gene063544

KEGG: K15111
GO: -
PFAM: PF00153.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063544.t1	XP_013451772.1	99.5	189	1	0	1	189	174	362	8.50E-98	366.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063544.t1	Q9FHX2	73.3	187	50	0	1	187	226	412	1.4e-72	273.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063544.t1	A0A072UJ24	99.5	189	1	0	1	189	174	362	6.1e-98	366.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene063544	0.811357	7.29E-51	-1.69E-46
MS.gene049382	MS.gene063544	0.813321	2.72E-51	-1.69E-46
MS.gene049703	MS.gene063544	-0.802468	5.53E-49	-1.69E-46
MS.gene049758	MS.gene063544	0.825244	5.21E-54	-1.69E-46
MS.gene04984	MS.gene063544	0.811888	5.59E-51	-1.69E-46
MS.gene049915	MS.gene063544	0.818961	1.49E-52	-1.69E-46
MS.gene050226	MS.gene063544	0.823133	1.63E-53	-1.69E-46
MS.gene050227	MS.gene063544	0.820921	5.31E-53	-1.69E-46
MS.gene050228	MS.gene063544	0.820165	7.92E-53	-1.69E-46
MS.gene050277	MS.gene063544	0.803707	3.07E-49	-1.69E-46
MS.gene050623	MS.gene063544	-0.838571	2.66E-57	-1.69E-46
MS.gene050642	MS.gene063544	0.812156	4.88E-51	-1.69E-46
MS.gene050762	MS.gene063544	0.80907	2.27E-50	-1.69E-46
MS.gene050763	MS.gene063544	0.812032	5.20E-51	-1.69E-46
MS.gene050987	MS.gene063544	0.804346	2.26E-49	-1.69E-46
MS.gene051243	MS.gene063544	0.800986	1.11E-48	-1.69E-46
MS.gene051245	MS.gene063544	0.820414	6.94E-53	-1.69E-46
MS.gene051535	MS.gene063544	0.816266	6.04E-52	-1.69E-46
MS.gene051662	MS.gene063544	0.809995	1.44E-50	-1.69E-46
MS.gene051983	MS.gene063544	0.806337	8.64E-50	-1.69E-46
MS.gene052176	MS.gene063544	0.811823	5.77E-51	-1.69E-46
MS.gene052302	MS.gene063544	0.862565	4.63E-64	-1.69E-46
MS.gene052309	MS.gene063544	-0.833434	5.35E-56	-1.69E-46
MS.gene052376	MS.gene063544	0.85963	3.62E-63	-1.69E-46
MS.gene052856	MS.gene063544	0.833184	6.17E-56	-1.69E-46
MS.gene052858	MS.gene063544	0.81146	6.92E-51	-1.69E-46
MS.gene052860	MS.gene063544	0.809015	2.33E-50	-1.69E-46
MS.gene052970	MS.gene063544	-0.813966	1.96E-51	-1.69E-46
MS.gene053216	MS.gene063544	0.818729	1.68E-52	-1.69E-46
MS.gene053559	MS.gene063544	0.823899	1.08E-53	-1.69E-46
MS.gene053836	MS.gene063544	0.823529	1.32E-53	-1.69E-46
MS.gene054379	MS.gene063544	0.830253	3.26E-55	-1.69E-46
MS.gene054930	MS.gene063544	0.846435	2.18E-59	-1.69E-46
MS.gene054942	MS.gene063544	0.807684	4.48E-50	-1.69E-46
MS.gene055133	MS.gene063544	0.813812	2.12E-51	-1.69E-46
MS.gene055174	MS.gene063544	0.800724	1.26E-48	-1.69E-46
MS.gene055413	MS.gene063544	0.813982	1.94E-51	-1.69E-46
MS.gene055441	MS.gene063544	0.805773	1.14E-49	-1.69E-46
MS.gene056069	MS.gene063544	-0.815886	7.35E-52	-1.69E-46
MS.gene056979	MS.gene063544	0.811649	6.30E-51	-1.69E-46
MS.gene057057	MS.gene063544	0.819359	1.21E-52	-1.69E-46
MS.gene057234	MS.gene063544	0.801837	7.46E-49	-1.69E-46
MS.gene057754	MS.gene063544	0.832993	6.88E-56	-1.69E-46
MS.gene058182	MS.gene063544	0.820657	6.11E-53	-1.69E-46
MS.gene058238	MS.gene063544	0.812573	3.96E-51	-1.69E-46
MS.gene058399	MS.gene063544	0.820591	6.32E-53	-1.69E-46
MS.gene058661	MS.gene063544	-0.814099	1.83E-51	-1.69E-46
MS.gene058749	MS.gene063544	0.819966	8.80E-53	-1.69E-46
MS.gene058833	MS.gene063544	0.835837	1.33E-56	-1.69E-46
MS.gene058845	MS.gene063544	0.812038	5.18E-51	-1.69E-46
MS.gene05984	MS.gene063544	0.807373	5.22E-50	-1.69E-46
MS.gene059894	MS.gene063544	0.805241	1.47E-49	-1.69E-46
MS.gene059899	MS.gene063544	0.844145	9.06E-59	-1.69E-46
MS.gene060084	MS.gene063544	0.831155	1.96E-55	-1.69E-46
MS.gene060297	MS.gene063544	0.811767	5.94E-51	-1.69E-46
MS.gene060476	MS.gene063544	0.823774	1.16E-53	-1.69E-46
MS.gene061113	MS.gene063544	0.804518	2.08E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063544.t1	MTR_6g034515	99.471	189	1	0	1	189	174	362	3.83e-137	387
MS.gene063544.t1	MTR_6g034515	26.738	187	117	6	3	185	83	253	6.72e-14	69.3
MS.gene063544.t1	MTR_1g097890	40.816	147	78	2	2	148	147	284	6.07e-30	112
MS.gene063544.t1	MTR_8g092080	40.816	147	78	2	2	148	148	285	9.97e-30	111
MS.gene063544.t1	MTR_8g098240	36.585	164	89	4	3	162	429	581	2.48e-25	102
MS.gene063544.t1	MTR_1g095780	33.889	180	105	4	2	179	629	796	1.12e-20	89.4
MS.gene063544.t1	MTR_2g102010	33.333	168	90	4	3	155	126	286	3.43e-19	83.2
MS.gene063544.t1	MTR_2g032610	34.524	168	88	6	1	153	144	304	4.23e-19	83.2
MS.gene063544.t1	MTR_2g032610	27.957	186	119	4	3	179	45	224	2.20e-15	73.2
MS.gene063544.t1	MTR_7g015870	29.082	196	105	8	1	180	140	317	2.25e-18	81.3
MS.gene063544.t1	MTR_7g015870	28.736	174	109	4	1	165	39	206	6.88e-15	71.6
MS.gene063544.t1	MTR_7g015870	28.994	169	95	6	1	153	136	295	6.59e-18	80.1
MS.gene063544.t1	MTR_7g015870	28.736	174	109	4	1	165	35	202	1.15e-14	71.2
MS.gene063544.t1	MTR_4g012850	30.108	186	114	4	1	177	124	302	1.86e-17	78.6
MS.gene063544.t1	MTR_4g119770	30.263	152	95	3	23	163	169	320	1.98e-17	79.0
MS.gene063544.t1	MTR_1g012030	27.749	191	125	4	1	189	153	332	1.99e-17	79.3
MS.gene063544.t1	MTR_1g012030	26.061	165	114	2	3	165	249	407	5.96e-15	72.4
MS.gene063544.t1	MTR_4g057850	33.962	159	88	4	1	148	131	283	7.76e-17	77.0
MS.gene063544.t1	MTR_4g114320	29.651	172	111	4	3	171	234	398	8.44e-17	77.4
MS.gene063544.t1	MTR_4g114320	28.571	189	118	4	1	186	137	311	1.12e-13	68.6
MS.gene063544.t1	MTR_7g064980	31.429	175	113	4	1	175	153	320	2.75e-16	75.5
MS.gene063544.t1	MTR_7g064980	26.347	167	105	4	25	186	75	228	1.04e-11	62.8
MS.gene063544.t1	MTR_3g107510	32.544	169	102	3	1	167	136	294	2.91e-16	75.9
MS.gene063544.t1	MTR_3g107510	25.731	171	119	2	3	171	232	396	4.21e-15	72.8
MS.gene063544.t1	MTR_2g436930	33.333	150	89	6	24	162	79	228	3.85e-16	73.9
MS.gene063544.t1	MTR_2g436930	33.333	150	89	6	24	162	169	318	6.53e-16	74.7
MS.gene063544.t1	MTR_6g091800	27.326	172	119	2	8	174	45	215	1.76e-15	72.8
MS.gene063544.t1	MTR_6g015860	27.374	179	103	5	1	162	132	300	1.88e-15	73.2
MS.gene063544.t1	MTR_6g015860	25.862	174	114	4	1	165	31	198	7.39e-11	60.1
MS.gene063544.t1	MTR_6g091800	27.326	172	119	2	8	174	45	215	2.54e-15	72.4
MS.gene063544.t1	MTR_6g091800	27.097	155	108	1	25	174	61	215	2.70e-15	72.8
MS.gene063544.t1	MTR_8g066975	31.667	180	102	4	3	168	124	296	4.37e-15	72.0
MS.gene063544.t1	MTR_8g096050	29.240	171	109	4	1	165	335	499	5.06e-15	72.8
MS.gene063544.t1	MTR_6g034145	27.097	155	108	1	25	174	7	161	6.02e-15	71.2
MS.gene063544.t1	MTR_3g104600	26.829	164	102	2	25	174	77	236	8.76e-15	71.6
MS.gene063544.t1	MTR_3g104600	33.553	152	87	5	11	158	181	322	5.34e-12	63.5
MS.gene063544.t1	MTR_6g092210	27.815	151	106	2	25	174	7	155	1.01e-14	70.5
MS.gene063544.t1	MTR_5g073040	29.121	182	118	5	1	177	147	322	1.96e-14	70.5
MS.gene063544.t1	MTR_5g073040	30.526	190	112	6	1	185	53	227	2.18e-13	67.4
MS.gene063544.t1	MTR_8g019480	30.366	191	88	8	3	155	147	330	3.80e-13	67.0
MS.gene063544.t1	MTR_3g025800	33.163	196	91	6	2	165	141	328	4.36e-13	66.6
MS.gene063544.t1	MTR_7g068340	26.786	168	110	2	21	188	131	285	6.64e-13	66.2
MS.gene063544.t1	MTR_7g084400	28.488	172	106	5	3	168	325	485	8.00e-13	66.2
MS.gene063544.t1	MTR_1g090330	26.974	152	100	3	3	147	30	177	1.66e-12	64.7
MS.gene063544.t1	MTR_6g091800	29.787	141	93	2	8	143	45	184	3.13e-12	62.8
MS.gene063544.t1	MTR_3g098460	26.316	171	114	4	1	165	313	477	1.38e-11	62.8
MS.gene063544.t1	MTR_4g005330	29.787	141	92	2	25	165	217	350	2.40e-11	61.6
MS.gene063544.t1	MTR_4g005330	29.787	141	92	2	25	165	151	284	2.42e-11	61.6
MS.gene063544.t1	MTR_8g086070	27.128	188	118	4	3	183	37	212	2.44e-11	60.5
MS.gene063544.t1	MTR_8g086070	27.835	194	118	5	3	189	37	215	2.73e-11	61.2
MS.gene063544.t1	MTR_8g027975	27.673	159	101	4	20	165	193	350	4.03e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063544.t1	AT5G42130	73.262	187	50	0	1	187	226	412	4.85e-97	287
MS.gene063544.t1	AT1G34065	40.136	147	79	2	2	148	171	308	6.57e-30	112
MS.gene063544.t1	AT1G34065	28.333	180	104	5	1	180	89	243	5.16e-11	60.8
MS.gene063544.t1	AT1G34065	40.136	147	79	2	2	148	178	315	6.64e-30	112
MS.gene063544.t1	AT1G34065	28.333	180	104	5	1	180	96	250	5.02e-11	60.8
MS.gene063544.t1	AT4G39460	41.497	147	77	2	2	148	154	291	1.01e-29	112
MS.gene063544.t1	AT4G39460	41.497	147	77	2	2	148	154	291	1.01e-29	112
MS.gene063544.t1	AT4G39460	41.497	147	77	2	2	148	154	291	1.01e-29	112
MS.gene063544.t1	AT4G11440	34.118	170	95	5	1	162	437	597	1.35e-22	94.7
MS.gene063544.t1	AT4G11440	34.118	170	95	5	1	162	437	597	1.35e-22	94.7
MS.gene063544.t1	AT2G37890	34.694	147	87	3	3	145	168	309	7.76e-19	82.8
MS.gene063544.t1	AT2G37890	27.976	168	102	4	25	186	92	246	2.17e-15	73.2
MS.gene063544.t1	AT2G35800	29.940	167	107	2	2	168	649	805	5.13e-18	81.6
MS.gene063544.t1	AT2G26360	30.286	175	109	3	2	173	220	384	5.83e-18	80.9
MS.gene063544.t1	AT3G53940	32.317	164	102	3	3	162	196	354	7.11e-18	80.5
MS.gene063544.t1	AT3G53940	30.588	170	95	6	25	186	120	274	1.22e-13	68.6
MS.gene063544.t1	AT4G32400	28.421	190	123	4	1	188	128	306	1.06e-17	80.1
MS.gene063544.t1	AT4G32400	26.506	166	112	3	3	165	224	382	7.41e-16	74.7
MS.gene063544.t1	AT5G01340	34.591	159	88	5	1	148	126	279	2.14e-17	78.6
MS.gene063544.t1	AT1G79900	31.792	173	106	3	1	171	124	286	3.36e-17	77.8
MS.gene063544.t1	AT5G15640	31.250	192	98	8	1	175	143	317	1.55e-16	76.3
MS.gene063544.t1	AT5G15640	29.379	177	110	4	1	168	42	212	1.50e-14	70.9
MS.gene063544.t1	AT5G15640	31.250	192	98	8	1	175	136	310	2.14e-16	75.9
MS.gene063544.t1	AT5G15640	29.379	177	110	4	1	168	35	205	1.80e-14	70.5
MS.gene063544.t1	AT1G14560	27.044	159	109	1	21	172	65	223	1.25e-15	73.6
MS.gene063544.t1	AT1G14560	27.044	159	109	1	21	172	65	223	1.36e-15	73.9
MS.gene063544.t1	AT3G21390	29.299	157	96	3	23	164	192	348	8.40e-15	71.6
MS.gene063544.t1	AT3G21390	29.299	157	96	3	23	164	170	326	8.61e-15	71.6
MS.gene063544.t1	AT4G26180	26.144	153	108	1	25	172	63	215	2.09e-14	70.1
MS.gene063544.t1	AT3G55640	27.976	168	102	4	25	186	85	239	2.29e-14	69.7
MS.gene063544.t1	AT3G55640	27.976	168	102	4	25	186	85	239	2.29e-14	69.7
MS.gene063544.t1	AT5G48970	29.870	154	94	4	23	162	104	257	2.53e-14	69.7
MS.gene063544.t1	AT4G26180	26.144	153	108	1	25	172	63	215	2.56e-14	70.1
MS.gene063544.t1	AT5G48970	29.870	154	94	4	23	162	175	328	3.73e-14	69.7
MS.gene063544.t1	AT3G55640	27.976	168	102	4	25	186	85	239	4.34e-14	69.7
MS.gene063544.t1	AT3G55640	30.357	168	108	3	3	166	161	323	4.78e-14	69.3
MS.gene063544.t1	AT3G55640	27.976	168	102	4	25	186	85	239	4.34e-14	69.7
MS.gene063544.t1	AT3G55640	30.357	168	108	3	3	166	161	323	4.78e-14	69.3
MS.gene063544.t1	AT3G53940	30.588	170	95	6	25	186	120	274	8.81e-14	68.2
MS.gene063544.t1	AT3G20240	29.139	151	98	2	25	174	203	345	1.84e-13	67.8
MS.gene063544.t1	AT1G07030	29.762	168	107	4	1	162	148	310	3.43e-13	67.0
MS.gene063544.t1	AT2G30160	27.222	180	105	5	1	165	150	318	1.15e-12	65.5
MS.gene063544.t1	AT1G14140	26.816	179	109	5	3	162	32	207	1.60e-12	65.1
MS.gene063544.t1	AT5G46800	28.962	183	106	4	3	168	124	299	1.73e-12	64.7
MS.gene063544.t1	AT5G46800	28.962	183	106	4	3	168	124	299	1.73e-12	64.7
MS.gene063544.t1	AT5G46800	28.962	183	106	4	3	168	124	299	1.73e-12	64.7
MS.gene063544.t1	AT1G72820	26.554	177	94	6	2	146	144	316	2.26e-12	64.7
MS.gene063544.t1	AT1G72820	26.554	177	94	6	2	146	144	316	2.26e-12	64.7
MS.gene063544.t1	AT5G64970	28.221	163	87	4	26	168	146	298	4.16e-12	63.9
MS.gene063544.t1	AT4G01100	24.309	181	119	3	3	165	111	291	4.86e-12	63.5
MS.gene063544.t1	AT1G74240	38.194	144	77	2	26	165	150	285	5.59e-12	63.5
MS.gene063544.t1	AT5G19760	30.405	148	99	3	3	150	34	177	5.61e-12	63.5
MS.gene063544.t1	AT4G01100	24.309	181	119	3	3	165	161	341	6.34e-12	63.5
MS.gene063544.t1	AT4G01100	27.742	155	100	3	23	171	83	231	3.16e-11	61.6
MS.gene063544.t1	AT5G64970	28.221	163	87	4	26	168	270	422	6.93e-12	63.5
MS.gene063544.t1	AT1G74240	36.667	150	83	2	20	165	192	333	7.21e-12	63.5
MS.gene063544.t1	AT4G01100	24.309	181	119	3	3	165	175	355	7.24e-12	63.5
MS.gene063544.t1	AT2G47490	26.471	170	106	5	1	162	136	294	1.36e-11	62.4
MS.gene063544.t1	AT4G03115	31.250	160	90	6	22	180	8	148	1.92e-11	61.2
MS.gene063544.t1	AT4G03115	31.250	160	90	6	22	180	16	156	2.13e-11	61.2
MS.gene063544.t1	AT4G03115	31.250	160	90	6	22	180	79	219	2.50e-11	61.6
MS.gene063544.t1	AT4G03115	31.250	160	90	6	22	180	111	251	4.04e-11	61.2
MS.gene063544.t1	AT3G51870	26.946	167	109	2	21	187	135	288	4.23e-11	61.2
MS.gene063544.t1	AT1G25380	30.137	146	83	5	25	162	166	300	5.39e-11	60.8
MS.gene063544.t1	AT1G25380	30.137	146	83	5	25	162	166	300	5.39e-11	60.8
MS.gene063544.t1	AT1G14560	28.082	146	100	2	21	165	78	219	6.61e-11	59.7

Find 54 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGCAGAATGAAGGTGTTT+TGG	0.264365	6.1:-14656320	MS.gene063544:CDS
GTGATTTGTGGGGCGTTGGC+GGG	0.323397	6.1:-14656163	MS.gene063544:CDS
TGTGATTTGTGGGGCGTTGG+CGG	0.357288	6.1:-14656164	MS.gene063544:CDS
GAGGAAGCAGTTGGAGGATG+TGG	0.369164	6.1:-14655876	MS.gene063544:CDS
GGTTATGTATGGTGGGGTTA+AGG	0.386748	6.1:-14656047	MS.gene063544:CDS
GCATACGAACAAAGATTGTT+TGG	0.390467	6.1:-14656200	MS.gene063544:CDS
AACATCCAACGGCGTAGTTA+TGG	0.407710	6.1:+14656126	None:intergenic
TGACTAGAGGAATGGGTCCT+AGG	0.415968	6.1:-14655982	MS.gene063544:CDS
CGGTTCAGAGTGTGATTTGT+GGG	0.419531	6.1:-14656174	MS.gene063544:CDS
TGATTTGTGGGGCGTTGGCG+GGG	0.437698	6.1:-14656162	MS.gene063544:CDS
CGCTGCGTTATCAATTCCTT+CGG	0.442471	6.1:+14656391	None:intergenic
TGTTGAGGAATTTGCCTGCT+GGG	0.444165	6.1:-14656270	MS.gene063544:CDS
AGGCGAGGAATGAGGCGGTT+GGG	0.475712	6.1:-14656081	MS.gene063544:CDS
GTGGGGTTAAGGATACTGTA+AGG	0.478723	6.1:-14656036	MS.gene063544:CDS
TTGTTGAGGAATTTGCCTGC+TGG	0.480628	6.1:-14656271	MS.gene063544:CDS
GTTGGTTTGACTAGAGGAAT+GGG	0.484116	6.1:-14655989	MS.gene063544:CDS
GGTTGGTTTGACTAGAGGAA+TGG	0.484380	6.1:-14655990	MS.gene063544:CDS
GATTTGTGGGGCGTTGGCGG+GGG	0.487414	6.1:-14656161	MS.gene063544:CDS
TTGAAGGAAGAAGGGTGGGT+TGG	0.489069	6.1:-14656007	MS.gene063544:CDS
TGTTAAGAGGAAGCAGTTGG+AGG	0.490136	6.1:-14655882	MS.gene063544:CDS
TTGCGGAGGTTATGTATGGT+GGG	0.491320	6.1:-14656054	MS.gene063544:CDS
CCACAAATCACACTCTGAAC+CGG	0.493103	6.1:+14656175	None:intergenic
GTTGCGGAGGTTATGTATGG+TGG	0.500010	6.1:-14656055	MS.gene063544:CDS
GTATGTTAAGAGGAAGCAGT+TGG	0.502406	6.1:-14655885	MS.gene063544:CDS
AAGGTTGCGGAGGTTATGTA+TGG	0.504862	6.1:-14656058	MS.gene063544:CDS
GACTAGAGGAATGGGTCCTA+GGG	0.505172	6.1:-14655981	MS.gene063544:CDS
GAGGAATGAGGCGGTTGGGA+AGG	0.510334	6.1:-14656077	MS.gene063544:CDS
CAGGCGAGGAATGAGGCGGT+TGG	0.514856	6.1:-14656082	MS.gene063544:CDS
CGGATGCAGACGGGTGCGAA+GGG	0.525730	6.1:-14656370	MS.gene063544:CDS
CCGGTTCAGAGTGTGATTTG+TGG	0.525919	6.1:-14656175	MS.gene063544:CDS
GAGTGTGATTTGTGGGGCGT+TGG	0.527587	6.1:-14656167	MS.gene063544:CDS
TGAGGCGGTTGGGAAGGTTG+CGG	0.528238	6.1:-14656071	MS.gene063544:CDS
GGCTTCCATAACTACGCCGT+TGG	0.535451	6.1:-14656131	MS.gene063544:CDS
GAACAAAGATTGTTTGGAGC+CGG	0.535824	6.1:-14656194	MS.gene063544:CDS
GGGTGGGTTGGTTTGACTAG+AGG	0.537595	6.1:-14655995	MS.gene063544:CDS
GATGACGCAGGCGAGGAATG+AGG	0.540893	6.1:-14656089	MS.gene063544:CDS
GCGGATGCAGACGGGTGCGA+AGG	0.558993	6.1:-14656371	MS.gene063544:CDS
TGCGGAGGTTATGTATGGTG+GGG	0.571518	6.1:-14656053	MS.gene063544:CDS
CAAGCACTATGAAGAACCCT+AGG	0.574254	6.1:+14655965	None:intergenic
GAATAACTTATAACCCCAGC+AGG	0.578876	6.1:+14656256	None:intergenic
GATAACGCAGCGGATGCAGA+CGG	0.581140	6.1:-14656380	MS.gene063544:CDS
ATAACGCAGCGGATGCAGAC+GGG	0.587923	6.1:-14656379	MS.gene063544:CDS
GTCTGCGATTATGGTTCCGA+AGG	0.591943	6.1:-14656407	None:intergenic
CGAGACTGATGACGCAGGCG+AGG	0.604725	6.1:-14656096	MS.gene063544:CDS
CTCGTCTTCACAACATCCAA+CGG	0.607204	6.1:+14656115	None:intergenic
GGCGGTTGGGAAGGTTGCGG+AGG	0.609437	6.1:-14656068	MS.gene063544:CDS
GTTGAGGAATTTGCCTGCTG+GGG	0.612034	6.1:-14656269	MS.gene063544:CDS
GGTACTCAGCTACATTGTTG+AGG	0.647888	6.1:-14656285	MS.gene063544:CDS
GGTTCAGAGTGTGATTTGTG+GGG	0.649669	6.1:-14656173	MS.gene063544:CDS
GGATGCAGACGGGTGCGAAG+GGG	0.664448	6.1:-14656369	MS.gene063544:CDS
TGGGGTTAAGGATACTGTAA+GGG	0.664936	6.1:-14656035	MS.gene063544:CDS
GACGCAGGCGAGGAATGAGG+CGG	0.666166	6.1:-14656086	MS.gene063544:CDS
GAAGACGAGACTGATGACGC+AGG	0.724323	6.1:-14656101	MS.gene063544:CDS
CGAAGGAATTGATAACGCAG+CGG	0.736123	6.1:-14656390	MS.gene063544:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAAGATTTTGCAGAATGA+AGG	-	chr6.1:14655919-14655938	MS.gene063544:CDS	25.0%
!!	TTTTGAATGAGTATGTTAAG+AGG	-	chr6.1:14656352-14656371	MS.gene063544:CDS	25.0%
!!	TCTTCATAGTGCTTGTTTTT+CGG	-	chr6.1:14656290-14656309	MS.gene063544:CDS	30.0%
	GCATACGAACAAAGATTGTT+TGG	-	chr6.1:14656047-14656066	MS.gene063544:CDS	35.0%
!	TACTGTAAGGGAGATTTTGA+AGG	-	chr6.1:14656224-14656243	MS.gene063544:CDS	35.0%
!!!	GTGTTTTGGGTTTGTATAGA+GGG	-	chr6.1:14655941-14655960	MS.gene063544:CDS	35.0%
!!!	TTGCAGAATGAAGGTGTTTT+GGG	-	chr6.1:14655928-14655947	MS.gene063544:CDS	35.0%
!!!	TTTGCAGAATGAAGGTGTTT+TGG	-	chr6.1:14655927-14655946	MS.gene063544:CDS	35.0%
	GAATAACTTATAACCCCAGC+AGG	+	chr6.1:14655994-14656013	None:intergenic	40.0%
	GTATGTTAAGAGGAAGCAGT+TGG	-	chr6.1:14656362-14656381	MS.gene063544:CDS	40.0%
	TGGGGTTAAGGATACTGTAA+GGG	-	chr6.1:14656212-14656231	MS.gene063544:CDS	40.0%
!	GAACAAAGATTGTTTGGAGC+CGG	-	chr6.1:14656053-14656072	MS.gene063544:CDS	40.0%
!!	AGATTTTGAAGGAAGAAGGG+TGG	-	chr6.1:14656235-14656254	MS.gene063544:CDS	40.0%
!!	AGGGAGATTTTGAAGGAAGA+AGG	-	chr6.1:14656231-14656250	MS.gene063544:CDS	40.0%
!!	AGTGCTTGTTTTTCGGCTTT+GGG	-	chr6.1:14656297-14656316	MS.gene063544:CDS	40.0%
!!	GATTTTGAAGGAAGAAGGGT+GGG	-	chr6.1:14656236-14656255	MS.gene063544:CDS	40.0%
!!	GGGAGATTTTGAAGGAAGAA+GGG	-	chr6.1:14656232-14656251	MS.gene063544:CDS	40.0%
!!	GTTGGTTTGACTAGAGGAAT+GGG	-	chr6.1:14656258-14656277	MS.gene063544:CDS	40.0%
!!	TAGTGCTTGTTTTTCGGCTT+TGG	-	chr6.1:14656296-14656315	MS.gene063544:CDS	40.0%
!!!	GGTGTTTTGGGTTTGTATAG+AGG	-	chr6.1:14655940-14655959	MS.gene063544:CDS	40.0%
	AACATCCAACGGCGTAGTTA+TGG	+	chr6.1:14656124-14656143	None:intergenic	45.0%
	AAGGTTGCGGAGGTTATGTA+TGG	-	chr6.1:14656189-14656208	MS.gene063544:CDS	45.0%
	CAAGCACTATGAAGAACCCT+AGG	+	chr6.1:14656285-14656304	None:intergenic	45.0%
	CCACAAATCACACTCTGAAC+CGG	+	chr6.1:14656075-14656094	None:intergenic	45.0%
	CGCTGCGTTATCAATTCCTT+CGG	+	chr6.1:14655859-14655878	None:intergenic	45.0%
	CTCGTCTTCACAACATCCAA+CGG	+	chr6.1:14656135-14656154	None:intergenic	45.0%
	GGTACTCAGCTACATTGTTG+AGG	-	chr6.1:14655962-14655981	MS.gene063544:CDS	45.0%
	GTGGGGTTAAGGATACTGTA+AGG	-	chr6.1:14656211-14656230	MS.gene063544:CDS	45.0%
	TGTTAAGAGGAAGCAGTTGG+AGG	-	chr6.1:14656365-14656384	MS.gene063544:CDS	45.0%
	TGTTGAGGAATTTGCCTGCT+GGG	-	chr6.1:14655977-14655996	MS.gene063544:CDS	45.0%
	TTGCGGAGGTTATGTATGGT+GGG	-	chr6.1:14656193-14656212	MS.gene063544:CDS	45.0%
	TTGTTGAGGAATTTGCCTGC+TGG	-	chr6.1:14655976-14655995	MS.gene063544:CDS	45.0%
!	CGGTTCAGAGTGTGATTTGT+GGG	-	chr6.1:14656073-14656092	MS.gene063544:CDS	45.0%
!	GGTTATGTATGGTGGGGTTA+AGG	-	chr6.1:14656200-14656219	MS.gene063544:CDS	45.0%
!	GGTTCAGAGTGTGATTTGTG+GGG	-	chr6.1:14656074-14656093	MS.gene063544:CDS	45.0%
!!	CGAAGGAATTGATAACGCAG+CGG	-	chr6.1:14655857-14655876	MS.gene063544:CDS	45.0%
!!	GGTTGGTTTGACTAGAGGAA+TGG	-	chr6.1:14656257-14656276	MS.gene063544:CDS	45.0%
	CCGGTTCAGAGTGTGATTTG+TGG	-	chr6.1:14656072-14656091	MS.gene063544:CDS	50.0%
	GACTAGAGGAATGGGTCCTA+GGG	-	chr6.1:14656266-14656285	MS.gene063544:CDS	50.0%
	GTTGAGGAATTTGCCTGCTG+GGG	-	chr6.1:14655978-14655997	MS.gene063544:CDS	50.0%
	GTTGCGGAGGTTATGTATGG+TGG	-	chr6.1:14656192-14656211	MS.gene063544:CDS	50.0%
	TGACTAGAGGAATGGGTCCT+AGG	-	chr6.1:14656265-14656284	MS.gene063544:CDS	50.0%
	TGCGGAGGTTATGTATGGTG+GGG	-	chr6.1:14656194-14656213	MS.gene063544:CDS	50.0%
	TTGAAGGAAGAAGGGTGGGT+TGG	-	chr6.1:14656240-14656259	MS.gene063544:CDS	50.0%
	ATAACGCAGCGGATGCAGAC+GGG	-	chr6.1:14655868-14655887	MS.gene063544:CDS	55.0%
	GAGGAAGCAGTTGGAGGATG+TGG	-	chr6.1:14656371-14656390	MS.gene063544:CDS	55.0%
	GATAACGCAGCGGATGCAGA+CGG	-	chr6.1:14655867-14655886	MS.gene063544:CDS	55.0%
	GGCTTCCATAACTACGCCGT+TGG	-	chr6.1:14656116-14656135	MS.gene063544:CDS	55.0%
!	GAAGACGAGACTGATGACGC+AGG	-	chr6.1:14656146-14656165	MS.gene063544:CDS	55.0%
!	GAGTGTGATTTGTGGGGCGT+TGG	-	chr6.1:14656080-14656099	MS.gene063544:CDS	55.0%
!!	GGGTGGGTTGGTTTGACTAG+AGG	-	chr6.1:14656252-14656271	MS.gene063544:CDS	55.0%
!!	TGTGATTTGTGGGGCGTTGG+CGG	-	chr6.1:14656083-14656102	MS.gene063544:CDS	55.0%
	AGGCGAGGAATGAGGCGGTT+GGG	-	chr6.1:14656166-14656185	MS.gene063544:CDS	60.0%
	GAGGAATGAGGCGGTTGGGA+AGG	-	chr6.1:14656170-14656189	MS.gene063544:CDS	60.0%
	GATGACGCAGGCGAGGAATG+AGG	-	chr6.1:14656158-14656177	MS.gene063544:CDS	60.0%
	TGAGGCGGTTGGGAAGGTTG+CGG	-	chr6.1:14656176-14656195	MS.gene063544:CDS	60.0%
!!	GTGATTTGTGGGGCGTTGGC+GGG	-	chr6.1:14656084-14656103	MS.gene063544:CDS	60.0%
!!	TGATTTGTGGGGCGTTGGCG+GGG	-	chr6.1:14656085-14656104	MS.gene063544:CDS	60.0%
	CAGGCGAGGAATGAGGCGGT+TGG	-	chr6.1:14656165-14656184	MS.gene063544:CDS	65.0%
	CGGATGCAGACGGGTGCGAA+GGG	-	chr6.1:14655877-14655896	MS.gene063544:CDS	65.0%
	GACGCAGGCGAGGAATGAGG+CGG	-	chr6.1:14656161-14656180	MS.gene063544:CDS	65.0%
	GGATGCAGACGGGTGCGAAG+GGG	-	chr6.1:14655878-14655897	MS.gene063544:CDS	65.0%
!	CGAGACTGATGACGCAGGCG+AGG	-	chr6.1:14656151-14656170	MS.gene063544:CDS	65.0%
!!	GATTTGTGGGGCGTTGGCGG+GGG	-	chr6.1:14656086-14656105	MS.gene063544:CDS	65.0%
	GCGGATGCAGACGGGTGCGA+AGG	-	chr6.1:14655876-14655895	MS.gene063544:CDS	70.0%
	GGCGGTTGGGAAGGTTGCGG+AGG	-	chr6.1:14656179-14656198	MS.gene063544:CDS	70.0%
!!	GGCGTTGGCGGGGGCGATAT+CGG	-	chr6.1:14656095-14656114	MS.gene063544:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	14655850	14656419	14655850	ID=MS.gene063544
chr6.1	mRNA	14655850	14656419	14655850	ID=MS.gene063544.t1;Parent=MS.gene063544
chr6.1	exon	14655850	14656419	14655850	ID=MS.gene063544.t1.exon1;Parent=MS.gene063544.t1
chr6.1	CDS	14655850	14656419	14655850	ID=cds.MS.gene063544.t1;Parent=MS.gene063544.t1

Gene Sequence

>MS.gene063544

ATGGTTCCGAAGGAATTGATAACGCAGCGGATGCAGACGGGTGCGAAGGGGAGATCGTACGAAGTTTTGATTAAGATTTTGCAGAATGAAGGTGTTTTGGGTTTGTATAGAGGGTACTCAGCTACATTGTTGAGGAATTTGCCTGCTGGGGTTATAAGTTATTCGTCGTTTGAGTATTTGAAGTTAGCGTTTATGAAGCATACGAACAAAGATTGTTTGGAGCCGGTTCAGAGTGTGATTTGTGGGGCGTTGGCGGGGGCGATATCGGCTTCCATAACTACGCCGTTGGATGTTGTGAAGACGAGACTGATGACGCAGGCGAGGAATGAGGCGGTTGGGAAGGTTGCGGAGGTTATGTATGGTGGGGTTAAGGATACTGTAAGGGAGATTTTGAAGGAAGAAGGGTGGGTTGGTTTGACTAGAGGAATGGGTCCTAGGGTTCTTCATAGTGCTTGTTTTTCGGCTTTGGGTTATTTTGCGTTTGAAACTGCTAGAGTTGCTATTTTGAATGAGTATGTTAAGAGGAAGCAGTTGGAGGATGTGGATGTTGTTGTTTCTAGTAGTAGTTGA

Protein sequence

>MS.gene063544.t1

MVPKELITQRMQTGAKGRSYEVLIKILQNEGVLGLYRGYSATLLRNLPAGVISYSSFEYLKLAFMKHTNKDCLEPVQSVICGALAGAISASITTPLDVVKTRLMTQARNEAVGKVAEVMYGGVKDTVREILKEEGWVGLTRGMGPRVLHSACFSALGYFAFETARVAILNEYVKRKQLEDVDVVVSSSS