AlfalfaGEDB | MS.gene26665

M. sativa cultivar XinJiangDaYe / MS.gene26665

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26665.t1	XP_013458408.1	90.4	208	20	0	1	208	1	208	4.00E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26665.t1	A0A072URM3	90.4	208	20	0	1	208	1	208	2.9e-64	254.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049133	MS.gene26665	0.813361	2.66E-51	-1.69E-46
MS.gene049418	MS.gene26665	0.871466	6.69E-67	-1.69E-46
MS.gene049622	MS.gene26665	0.812286	4.58E-51	-1.69E-46
MS.gene049660	MS.gene26665	0.814632	1.40E-51	-1.69E-46
MS.gene049697	MS.gene26665	0.815878	7.38E-52	-1.69E-46
MS.gene04985	MS.gene26665	0.803266	3.78E-49	-1.69E-46
MS.gene049989	MS.gene26665	0.884187	2.38E-71	-1.69E-46
MS.gene050297	MS.gene26665	0.869696	2.55E-66	-1.69E-46
MS.gene050310	MS.gene26665	0.823639	1.24E-53	-1.69E-46
MS.gene05054	MS.gene26665	0.810134	1.34E-50	-1.69E-46
MS.gene050648	MS.gene26665	0.813266	2.79E-51	-1.69E-46
MS.gene051067	MS.gene26665	0.821138	4.74E-53	-1.69E-46
MS.gene051263	MS.gene26665	0.855286	6.99E-62	-1.69E-46
MS.gene051423	MS.gene26665	0.816603	5.08E-52	-1.69E-46
MS.gene051478	MS.gene26665	0.867669	1.15E-65	-1.69E-46
MS.gene051629	MS.gene26665	0.829162	6.01E-55	-1.69E-46
MS.gene051648	MS.gene26665	0.801584	8.40E-49	-1.69E-46
MS.gene051742	MS.gene26665	0.814637	1.39E-51	-1.69E-46
MS.gene052143	MS.gene26665	0.839823	1.26E-57	-1.69E-46
MS.gene052308	MS.gene26665	0.805624	1.22E-49	-1.69E-46
MS.gene052367	MS.gene26665	0.868074	8.55E-66	-1.69E-46
MS.gene053173	MS.gene26665	0.824189	9.24E-54	-1.69E-46
MS.gene053348	MS.gene26665	0.809037	2.31E-50	-1.69E-46
MS.gene053416	MS.gene26665	0.836996	6.74E-57	-1.69E-46
MS.gene053417	MS.gene26665	0.838963	2.10E-57	-1.69E-46
MS.gene053500	MS.gene26665	0.815444	9.22E-52	-1.69E-46
MS.gene053536	MS.gene26665	0.818989	1.47E-52	-1.69E-46
MS.gene053538	MS.gene26665	0.820127	8.08E-53	-1.69E-46
MS.gene05369	MS.gene26665	0.804328	2.28E-49	-1.69E-46
MS.gene054551	MS.gene26665	0.834547	2.81E-56	-1.69E-46
MS.gene054614	MS.gene26665	0.835492	1.63E-56	-1.69E-46
MS.gene054643	MS.gene26665	0.812345	4.44E-51	-1.69E-46
MS.gene054999	MS.gene26665	0.808161	3.55E-50	-1.69E-46
MS.gene055308	MS.gene26665	0.804341	2.26E-49	-1.69E-46
MS.gene055578	MS.gene26665	0.803873	2.83E-49	-1.69E-46
MS.gene055777	MS.gene26665	0.801531	8.62E-49	-1.69E-46
MS.gene055957	MS.gene26665	0.865796	4.55E-65	-1.69E-46
MS.gene056041	MS.gene26665	0.863705	2.06E-64	-1.69E-46
MS.gene056042	MS.gene26665	0.849338	3.45E-60	-1.69E-46
MS.gene056228	MS.gene26665	0.831355	1.75E-55	-1.69E-46
MS.gene056526	MS.gene26665	0.805964	1.04E-49	-1.69E-46
MS.gene056704	MS.gene26665	0.818929	1.52E-52	-1.69E-46
MS.gene056870	MS.gene26665	0.815595	8.53E-52	-1.69E-46
MS.gene05709	MS.gene26665	0.853617	2.12E-61	-1.69E-46
MS.gene057483	MS.gene26665	0.834067	3.71E-56	-1.69E-46
MS.gene058196	MS.gene26665	0.826582	2.51E-54	-1.69E-46
MS.gene058197	MS.gene26665	0.8442	8.76E-59	-1.69E-46
MS.gene058198	MS.gene26665	0.84294	1.90E-58	-1.69E-46
MS.gene05866	MS.gene26665	0.828847	7.16E-55	-1.69E-46
MS.gene058818	MS.gene26665	0.805977	1.03E-49	-1.69E-46
MS.gene058853	MS.gene26665	0.802453	5.57E-49	-1.69E-46
MS.gene059163	MS.gene26665	0.8097	1.66E-50	-1.69E-46
MS.gene059164	MS.gene26665	0.80073	1.26E-48	-1.69E-46
MS.gene059300	MS.gene26665	0.878266	3.23E-69	-1.69E-46
MS.gene05930	MS.gene26665	0.805459	1.32E-49	-1.69E-46
MS.gene059424	MS.gene26665	0.804598	2.00E-49	-1.69E-46
MS.gene060473	MS.gene26665	0.852722	3.83E-61	-1.69E-46
MS.gene060565	MS.gene26665	0.848691	5.22E-60	-1.69E-46
MS.gene06057	MS.gene26665	0.860283	2.30E-63	-1.69E-46
MS.gene060825	MS.gene26665	0.816528	5.28E-52	-1.69E-46
MS.gene06093	MS.gene26665	0.803224	3.86E-49	-1.69E-46
MS.gene061099	MS.gene26665	0.821567	3.77E-53	-1.69E-46
MS.gene061260	MS.gene26665	0.841096	5.85E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26665.t1	MTR_4g125370	97.596	208	5	0	1	208	1	208	3.73e-149	412

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26665.t1	AT3G42725	47.525	202	99	2	7	207	6	201	4.24e-57	179

Find 43 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCAATAGTTTAGCTTCTTT+TGG	0.129943	8.3:-4757473	None:intergenic
GAGAAAATGAAAAGCAAATA+TGG	0.232910	8.3:+4757716	MS.gene26665:CDS
TTTGGTAAACAAAGCAAATA+TGG	0.284232	8.3:-4757411	None:intergenic
TTGGAGGCTTGCATTCTCTT+TGG	0.331209	8.3:-4757429	None:intergenic
CTTACACTAGCCTCTTTGAT+AGG	0.334310	8.3:+4757377	MS.gene26665:CDS
CCCTTGATGACTTTAACATT+TGG	0.375463	8.3:-4757448	None:intergenic
TTGCAAGAAAGAGGAGTAGA+AGG	0.398153	8.3:+4757621	MS.gene26665:CDS
AAAATGCCACCAACTTCATT+TGG	0.415049	8.3:+4757257	MS.gene26665:CDS
TTGATGACTTTAACATTTGG+AGG	0.415123	8.3:-4757445	None:intergenic
TATGTTGCAGTTTGTTGTGT+TGG	0.427873	8.3:+4757529	MS.gene26665:CDS
TGAAATTCAAAGAGCAAAGA+AGG	0.444128	8.3:-4757187	None:intergenic
TGAAAAGCAAATATGGGCAG+TGG	0.445200	8.3:+4757723	MS.gene26665:CDS
CCCAAAAGAAGCTAAACTAT+TGG	0.454597	8.3:+4757472	MS.gene26665:CDS
CTATCAAAGAGGCTAGTGTA+AGG	0.458593	8.3:-4757376	None:intergenic
ATTTGGCATGGCATTCCTCA+TGG	0.474932	8.3:+4757274	MS.gene26665:CDS
AGAAAATGAAAAGCAAATAT+GGG	0.492982	8.3:+4757717	MS.gene26665:CDS
TCCAAATGTTAAAGTCATCA+AGG	0.501062	8.3:+4757447	MS.gene26665:CDS
GATTGACGAAATTAAAGATA+AGG	0.504450	8.3:+4757685	MS.gene26665:CDS
AGAACTTGAAGCTGAGAAAG+AGG	0.510711	8.3:+4757595	MS.gene26665:CDS
TATTGGTAAGGCTTGAATGT+GGG	0.529499	8.3:+4757489	MS.gene26665:CDS
AAGAAGCTAAACTATTGGTA+AGG	0.535909	8.3:+4757477	MS.gene26665:CDS
AAGAGGCTATTGCAAGAAAG+AGG	0.537999	8.3:+4757612	MS.gene26665:CDS
GCCACCAACTTCATTTGGCA+TGG	0.540473	8.3:+4757262	MS.gene26665:CDS
ATTGGTAAGGCTTGAATGTG+GGG	0.545428	8.3:+4757490	MS.gene26665:CDS
GCAAAGAAGGAGGACGGTGA+AGG	0.548740	8.3:-4757174	None:intergenic
AATCTTAGCTACATTTGCAG+TGG	0.558481	8.3:-4757666	None:intergenic
GAAAAGCAAATATGGGCAGT+GGG	0.567953	8.3:+4757724	MS.gene26665:CDS
AAGGATTTCTAAAGTGCAAG+AGG	0.585321	8.3:+4757640	MS.gene26665:CDS
GAAAATTTGTCCTATCAAAG+AGG	0.589405	8.3:-4757387	None:intergenic
GAGAATGGTTGGTGCAAATG+AGG	0.594224	8.3:-4757231	None:intergenic
AATGCCATGCCAAATGAAGT+TGG	0.603497	8.3:-4757266	None:intergenic
TATCAAAGAGGCTAGTGTAA+GGG	0.605138	8.3:-4757375	None:intergenic
AGACGAGAAAAGTGAGATGG+TGG	0.612626	8.3:-4757300	None:intergenic
CCAAATGTTAAAGTCATCAA+GGG	0.626958	8.3:+4757448	MS.gene26665:CDS
CAAAGAGCAAAGAAGGAGGA+CGG	0.630557	8.3:-4757180	None:intergenic
CTATTGGTAAGGCTTGAATG+TGG	0.636368	8.3:+4757488	MS.gene26665:CDS
TTGTGTAGTGCATAGTTGTG+TGG	0.646229	8.3:+4757559	MS.gene26665:CDS
GAAAGACGAGAAAAGTGAGA+TGG	0.658650	8.3:-4757303	None:intergenic
TGAGAAAACACAAGTGTGTG+AGG	0.679362	8.3:-4757343	None:intergenic
AATTCAAAGAGCAAAGAAGG+AGG	0.681520	8.3:-4757184	None:intergenic
GTGAGATGGTGGAAACCATG+AGG	0.690704	8.3:-4757289	None:intergenic
GCCATGCCAAATGAAGTTGG+TGG	0.694987	8.3:-4757263	None:intergenic
GTGCAAATGAGGTAGCACAA+AGG	0.723598	8.3:-4757220	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAAATGAAAAGCAAATAT+GGG	+	chr8.3:4757717-4757736	MS.gene26665:CDS	20.0%
!	GAGAAAATGAAAAGCAAATA+TGG	+	chr8.3:4757716-4757735	MS.gene26665:CDS	25.0%
!	GATTGACGAAATTAAAGATA+AGG	+	chr8.3:4757685-4757704	MS.gene26665:CDS	25.0%
!	TTTGGTAAACAAAGCAAATA+TGG	-	chr8.3:4757414-4757433	None:intergenic	25.0%
	AAGAAGCTAAACTATTGGTA+AGG	+	chr8.3:4757477-4757496	MS.gene26665:CDS	30.0%
	CCAAATGTTAAAGTCATCAA+GGG	+	chr8.3:4757448-4757467	MS.gene26665:CDS	30.0%
	GAAAATTTGTCCTATCAAAG+AGG	-	chr8.3:4757390-4757409	None:intergenic	30.0%
	TCCAAATGTTAAAGTCATCA+AGG	+	chr8.3:4757447-4757466	MS.gene26665:CDS	30.0%
	TGAAATTCAAAGAGCAAAGA+AGG	-	chr8.3:4757190-4757209	None:intergenic	30.0%
!	TTGATGACTTTAACATTTGG+AGG	-	chr8.3:4757448-4757467	None:intergenic	30.0%
!!	ACCAATAGTTTAGCTTCTTT+TGG	-	chr8.3:4757476-4757495	None:intergenic	30.0%
!!	CCAATAGTTTAGCTTCTTTT+GGG	-	chr8.3:4757475-4757494	None:intergenic	30.0%
	AAAATGCCACCAACTTCATT+TGG	+	chr8.3:4757257-4757276	MS.gene26665:CDS	35.0%
	AATCTTAGCTACATTTGCAG+TGG	-	chr8.3:4757669-4757688	None:intergenic	35.0%
	AATTCAAAGAGCAAAGAAGG+AGG	-	chr8.3:4757187-4757206	None:intergenic	35.0%
	CCCAAAAGAAGCTAAACTAT+TGG	+	chr8.3:4757472-4757491	MS.gene26665:CDS	35.0%
	TATCAAAGAGGCTAGTGTAA+GGG	-	chr8.3:4757378-4757397	None:intergenic	35.0%
	TATTGGTAAGGCTTGAATGT+GGG	+	chr8.3:4757489-4757508	MS.gene26665:CDS	35.0%
!	CCCTTGATGACTTTAACATT+TGG	-	chr8.3:4757451-4757470	None:intergenic	35.0%
!	GGAACATGCTTTTCTAATCT+TGG	-	chr8.3:4757156-4757175	None:intergenic	35.0%
!	TGCTTTTCTAATCTTGGAAG+AGG	-	chr8.3:4757150-4757169	None:intergenic	35.0%
!!	AAGGATTTCTAAAGTGCAAG+AGG	+	chr8.3:4757640-4757659	MS.gene26665:CDS	35.0%
!!	TATGTTGCAGTTTGTTGTGT+TGG	+	chr8.3:4757529-4757548	MS.gene26665:CDS	35.0%
	AAGAGGCTATTGCAAGAAAG+AGG	+	chr8.3:4757612-4757631	MS.gene26665:CDS	40.0%
	AATGCCATGCCAAATGAAGT+TGG	-	chr8.3:4757269-4757288	None:intergenic	40.0%
	AGAACTTGAAGCTGAGAAAG+AGG	+	chr8.3:4757595-4757614	MS.gene26665:CDS	40.0%
	ATTGGTAAGGCTTGAATGTG+GGG	+	chr8.3:4757490-4757509	MS.gene26665:CDS	40.0%
	CTATCAAAGAGGCTAGTGTA+AGG	-	chr8.3:4757379-4757398	None:intergenic	40.0%
	CTATTGGTAAGGCTTGAATG+TGG	+	chr8.3:4757488-4757507	MS.gene26665:CDS	40.0%
	GAAAAGCAAATATGGGCAGT+GGG	+	chr8.3:4757724-4757743	MS.gene26665:CDS	40.0%
	GAAAGACGAGAAAAGTGAGA+TGG	-	chr8.3:4757306-4757325	None:intergenic	40.0%
	TGAAAAGCAAATATGGGCAG+TGG	+	chr8.3:4757723-4757742	MS.gene26665:CDS	40.0%
	TGAGAAAACACAAGTGTGTG+AGG	-	chr8.3:4757346-4757365	None:intergenic	40.0%
	TTGCAAGAAAGAGGAGTAGA+AGG	+	chr8.3:4757621-4757640	MS.gene26665:CDS	40.0%
	TTGTGTAGTGCATAGTTGTG+TGG	+	chr8.3:4757559-4757578	MS.gene26665:CDS	40.0%
!	CTTACACTAGCCTCTTTGAT+AGG	+	chr8.3:4757377-4757396	MS.gene26665:CDS	40.0%
!!	CTTGAATGTGGGGTTTTGAT+GGG	+	chr8.3:4757500-4757519	MS.gene26665:CDS	40.0%
!!	TCACTTTTCTCGTCTTTCGT+CGG	+	chr8.3:4757308-4757327	MS.gene26665:CDS	40.0%
!!!	ATGAAGTTGGTGGCATTTTG+AGG	-	chr8.3:4757256-4757275	None:intergenic	40.0%
	AGACGAGAAAAGTGAGATGG+TGG	-	chr8.3:4757303-4757322	None:intergenic	45.0%
	ATTTGGCATGGCATTCCTCA+TGG	+	chr8.3:4757274-4757293	MS.gene26665:CDS	45.0%
	CAAAGAGCAAAGAAGGAGGA+CGG	-	chr8.3:4757183-4757202	None:intergenic	45.0%
	GTGCAAATGAGGTAGCACAA+AGG	-	chr8.3:4757223-4757242	None:intergenic	45.0%
	TTGGAGGCTTGCATTCTCTT+TGG	-	chr8.3:4757432-4757451	None:intergenic	45.0%
!	GAGAATGGTTGGTGCAAATG+AGG	-	chr8.3:4757234-4757253	None:intergenic	45.0%
!!	GCTTGAATGTGGGGTTTTGA+TGG	+	chr8.3:4757499-4757518	MS.gene26665:CDS	45.0%
!!	GGCATTTTGAGGAGAATGGT+TGG	-	chr8.3:4757245-4757264	None:intergenic	45.0%
!!	TGGTGGCATTTTGAGGAGAA+TGG	-	chr8.3:4757249-4757268	None:intergenic	45.0%
	GCCACCAACTTCATTTGGCA+TGG	+	chr8.3:4757262-4757281	MS.gene26665:CDS	50.0%
	GCCATGCCAAATGAAGTTGG+TGG	-	chr8.3:4757266-4757285	None:intergenic	50.0%
!!	GTGAGATGGTGGAAACCATG+AGG	-	chr8.3:4757292-4757311	None:intergenic	50.0%
	GCAAAGAAGGAGGACGGTGA+AGG	-	chr8.3:4757177-4757196	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	4757143	4757769	4757143	ID=MS.gene26665
chr8.3	mRNA	4757143	4757769	4757143	ID=MS.gene26665.t1;Parent=MS.gene26665
chr8.3	exon	4757143	4757769	4757143	ID=MS.gene26665.t1.exon1;Parent=MS.gene26665.t1
chr8.3	CDS	4757143	4757769	4757143	ID=cds.MS.gene26665.t1;Parent=MS.gene26665.t1

Gene Sequence

>MS.gene26665

ATGGCCTCTTCCAAGATTAGAAAAGCATGTTCCTTCACCGTCCTCCTTCTTTGCTCTTTGAATTTCACACTTTTTATCCTTTGTGCTACCTCATTTGCACCAACCATTCTCCTCAAAATGCCACCAACTTCATTTGGCATGGCATTCCTCATGGTTTCCACCATCTCACTTTTCTCGTCTTTCGTCGGCTTCTATTCACACCTCACACACTTGTGTTTTCTCACTCACATTTCCCTTACACTAGCCTCTTTGATAGGACAAATTTTCACCATATTTGCTTTGTTTACCAAAGAGAATGCAAGCCTCCAAATGTTAAAGTCATCAAGGGACCCAAAAGAAGCTAAACTATTGGTAAGGCTTGAATGTGGGGTTTTGATGGGAATGTGTATGTTGCAGTTTGTTGTGTTGGTGTTGAGTTGTGTAGTGCATAGTTGTGTGGTGAAAGATTATGAAGAACTTGAAGCTGAGAAAGAGGCTATTGCAAGAAAGAGGAGTAGAAGGATTTCTAAAGTGCAAGAGGAATCCACTGCAAATGTAGCTAAGATTGACGAAATTAAAGATAAGGAGTTCGATGAGAAAATGAAAAGCAAATATGGGCAGTGGGTAAAAACTGATTTTGAACCATGA

Protein sequence

>MS.gene26665.t1

MASSKIRKACSFTVLLLCSLNFTLFILCATSFAPTILLKMPPTSFGMAFLMVSTISLFSSFVGFYSHLTHLCFLTHISLTLASLIGQIFTIFALFTKENASLQMLKSSRDPKEAKLLVRLECGVLMGMCMLQFVVLVLSCVVHSCVVKDYEELEAEKEAIARKRSRRISKVQEESTANVAKIDEIKDKEFDEKMKSKYGQWVKTDFEP