Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32024.t1 | XP_013456959.1 | 86.9 | 359 | 43 | 2 | 1 | 356 | 1 | 358 | 1.70E-83 | 319.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32024.t1 | Q01197 | 41.7 | 120 | 56 | 5 | 8 | 116 | 10 | 126 | 2.9e-07 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32024.t1 | I3T3J6 | 86.9 | 359 | 43 | 2 | 1 | 356 | 1 | 358 | 1.2e-83 | 319.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene32024 | 0.804462 | 2.14E-49 | -1.69E-46 |
| MS.gene049418 | MS.gene32024 | 0.883402 | 4.63E-71 | -1.69E-46 |
| MS.gene049698 | MS.gene32024 | 0.805416 | 1.35E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene32024 | 0.818836 | 1.59E-52 | -1.69E-46 |
| MS.gene049989 | MS.gene32024 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene050297 | MS.gene32024 | 0.801418 | 9.09E-49 | -1.69E-46 |
| MS.gene050310 | MS.gene32024 | 0.84212 | 3.14E-58 | -1.69E-46 |
| MS.gene050589 | MS.gene32024 | 0.812124 | 4.97E-51 | -1.69E-46 |
| MS.gene050649 | MS.gene32024 | 0.803353 | 3.63E-49 | -1.69E-46 |
| MS.gene051007 | MS.gene32024 | 0.813844 | 2.08E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene32024 | 0.841196 | 5.50E-58 | -1.69E-46 |
| MS.gene051021 | MS.gene32024 | 0.825551 | 4.41E-54 | -1.69E-46 |
| MS.gene051067 | MS.gene32024 | 0.844517 | 7.20E-59 | -1.69E-46 |
| MS.gene051478 | MS.gene32024 | 0.847448 | 1.15E-59 | -1.69E-46 |
| MS.gene051648 | MS.gene32024 | 0.825298 | 5.06E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene32024 | 0.810137 | 1.34E-50 | -1.69E-46 |
| MS.gene052731 | MS.gene32024 | 0.80987 | 1.53E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene32024 | 0.865855 | 4.36E-65 | -1.69E-46 |
| MS.gene053536 | MS.gene32024 | 0.873494 | 1.41E-67 | -1.69E-46 |
| MS.gene053538 | MS.gene32024 | 0.871586 | 6.10E-67 | -1.69E-46 |
| MS.gene053945 | MS.gene32024 | 0.81493 | 1.20E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene32024 | 0.87111 | 8.77E-67 | -1.69E-46 |
| MS.gene055537 | MS.gene32024 | 0.860063 | 2.68E-63 | -1.69E-46 |
| MS.gene055580 | MS.gene32024 | -0.800326 | 1.52E-48 | -1.69E-46 |
| MS.gene055764 | MS.gene32024 | 0.811276 | 7.59E-51 | -1.69E-46 |
| MS.gene055777 | MS.gene32024 | 0.827341 | 1.65E-54 | -1.69E-46 |
| MS.gene056169 | MS.gene32024 | 0.839034 | 2.02E-57 | -1.69E-46 |
| MS.gene056228 | MS.gene32024 | 0.854563 | 1.13E-61 | -1.69E-46 |
| MS.gene056396 | MS.gene32024 | 0.800421 | 1.45E-48 | -1.69E-46 |
| MS.gene056704 | MS.gene32024 | 0.816528 | 5.28E-52 | -1.69E-46 |
| MS.gene057082 | MS.gene32024 | 0.817816 | 2.71E-52 | -1.69E-46 |
| MS.gene05709 | MS.gene32024 | 0.813395 | 2.62E-51 | -1.69E-46 |
| MS.gene058196 | MS.gene32024 | 0.814317 | 1.64E-51 | -1.69E-46 |
| MS.gene058197 | MS.gene32024 | 0.866136 | 3.55E-65 | -1.69E-46 |
| MS.gene058198 | MS.gene32024 | 0.866393 | 2.95E-65 | -1.69E-46 |
| MS.gene058823 | MS.gene32024 | 0.801212 | 1.00E-48 | -1.69E-46 |
| MS.gene05894 | MS.gene32024 | 0.846622 | 1.93E-59 | -1.69E-46 |
| MS.gene059300 | MS.gene32024 | 0.847557 | 1.07E-59 | -1.69E-46 |
| MS.gene059424 | MS.gene32024 | 0.87157 | 6.18E-67 | -1.69E-46 |
| MS.gene059425 | MS.gene32024 | 0.807325 | 5.34E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene32024 | 0.831037 | 2.09E-55 | -1.69E-46 |
| MS.gene06057 | MS.gene32024 | 0.860003 | 2.79E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32024.t1 | MTR_4g088385 | 88.611 | 360 | 35 | 3 | 1 | 356 | 1 | 358 | 4.72e-146 | 417 |
| MS.gene32024.t1 | MTR_2g014400 | 52.381 | 147 | 56 | 5 | 217 | 355 | 120 | 260 | 2.17e-17 | 81.3 |
| MS.gene32024.t1 | MTR_2g014400 | 57.317 | 82 | 30 | 3 | 1 | 80 | 1 | 79 | 1.47e-13 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTTCTGGTTTGTTTAA+TGG | 0.166365 | 4.3:+25004966 | None:intergenic |
| GACTGTCTCTGGCATGAATT+TGG | 0.206853 | 4.3:+25005057 | None:intergenic |
| TTCGAGTTATATCCTTCTTC+TGG | 0.209481 | 4.3:+25004621 | None:intergenic |
| ATTCGGGTACTTGTTGTTAT+AGG | 0.218132 | 4.3:+25004755 | None:intergenic |
| CTTCAAACTGCTCTTGATTC+TGG | 0.250818 | 4.3:+25004094 | None:intergenic |
| TTCAAGGCTCAAATTCTTCT+TGG | 0.258570 | 4.3:+25004067 | None:intergenic |
| TTTAATGGTGTTTCATTGTT+AGG | 0.261763 | 4.3:+25004981 | None:intergenic |
| AGAAATACTTGTTGTTGTTC+TGG | 0.298090 | 4.3:+25004487 | None:intergenic |
| ATGAAAACTGGTTGTTGTTC+TGG | 0.303072 | 4.3:+25004954 | None:intergenic |
| CAAGTATTCTTCTAGTTCTA+AGG | 0.327940 | 4.3:-25004664 | MS.gene32024:CDS |
| TTGATTCTGGTAACCATTCA+TGG | 0.329924 | 4.3:+25004107 | None:intergenic |
| GAAAACAGTTATGGTTTATA+TGG | 0.334786 | 4.3:-25004921 | MS.gene32024:CDS |
| GGTTTATATGGTCATGATAC+TGG | 0.336006 | 4.3:-25004909 | MS.gene32024:CDS |
| ATTGAGTTGTAACCTTCTTC+TGG | 0.340437 | 4.3:+25004429 | None:intergenic |
| TTCATATGATTGGAAAGTGT+TGG | 0.393181 | 4.3:+25004845 | None:intergenic |
| TCGGTTTCCGGGATGAAAAC+TGG | 0.395977 | 4.3:+25004942 | None:intergenic |
| GCTGAAGAATTGACTGTCTC+TGG | 0.398942 | 4.3:+25005046 | None:intergenic |
| TTGGTGTCACTAACTTCATT+CGG | 0.404949 | 4.3:+25004738 | None:intergenic |
| TGTTGTTATAGGAATCTTGC+TGG | 0.405186 | 4.3:+25004766 | None:intergenic |
| GTAGTTGTAGTTGTTGTTGT+TGG | 0.409889 | 4.3:+25004695 | None:intergenic |
| TCATATGATTGGAAAGTGTT+GGG | 0.422874 | 4.3:+25004846 | None:intergenic |
| CCGGAAACCGAAAACAGTTA+TGG | 0.426235 | 4.3:-25004930 | MS.gene32024:CDS |
| TCGAGTTATATCCTTCTTCT+GGG | 0.432731 | 4.3:+25004622 | None:intergenic |
| TGGTGTCACTAACTTCATTC+GGG | 0.435111 | 4.3:+25004739 | None:intergenic |
| TTGAGTTGTAACCTTCTTCT+GGG | 0.440468 | 4.3:+25004430 | None:intergenic |
| GTAAGATGTTCCCGTTAGTT+TGG | 0.449964 | 4.3:+25004719 | None:intergenic |
| AACGCTAAGTTTACTGAAGA+AGG | 0.456600 | 4.3:-25004528 | MS.gene32024:CDS |
| TTCAATGGAGATTCATCTAA+AGG | 0.457617 | 4.3:-25004213 | MS.gene32024:CDS |
| AATGAGTGACACAAGAGTTA+TGG | 0.465302 | 4.3:-25004289 | MS.gene32024:CDS |
| ATGAAAGGGTTGTTAAGAAT+AGG | 0.475071 | 4.3:+25005087 | None:intergenic |
| AAATACAATCCAACATTCAA+TGG | 0.477159 | 4.3:-25004228 | MS.gene32024:CDS |
| TTGTTTGTGTTAGTAGTACT+AGG | 0.484396 | 4.3:+25004876 | None:intergenic |
| GTTAGTGACACCAAACTAAC+GGG | 0.485500 | 4.3:-25004729 | MS.gene32024:CDS |
| GAGTTGTGAACTCTGAGAAT+TGG | 0.491359 | 4.3:-25004191 | MS.gene32024:CDS |
| GATGAATCTCCATTGAATGT+TGG | 0.492045 | 4.3:+25004219 | None:intergenic |
| TTGTTAAGAATAGGAAAGAA+AGG | 0.493863 | 4.3:+25005096 | None:intergenic |
| TTGTGTTAGTAGTACTAGGA+GGG | 0.504780 | 4.3:+25004880 | None:intergenic |
| AACACAAAGTTCCCAGAAGA+AGG | 0.504928 | 4.3:-25004441 | MS.gene32024:CDS |
| GAATTTGGAGAGAAAATGAA+AGG | 0.529100 | 4.3:+25005072 | None:intergenic |
| AATAACAAGAACTCCATGAA+TGG | 0.530258 | 4.3:-25004120 | MS.gene32024:CDS |
| TACAACAATGGTGAGAGGCA+AGG | 0.586180 | 4.3:-25004312 | MS.gene32024:CDS |
| TGAAGAAGGATACAAATCCA+TGG | 0.589242 | 4.3:-25004514 | MS.gene32024:CDS |
| TTTGTGTTAGTAGTACTAGG+AGG | 0.589979 | 4.3:+25004879 | None:intergenic |
| AATTTGGAGAGAAAATGAAA+GGG | 0.593601 | 4.3:+25005073 | None:intergenic |
| AGTTAGTGACACCAAACTAA+CGG | 0.603223 | 4.3:-25004730 | MS.gene32024:CDS |
| AGTAACAACAATTACAACAA+TGG | 0.621284 | 4.3:-25004324 | MS.gene32024:CDS |
| GACACAAGAGTTATGGAAGG+TGG | 0.628338 | 4.3:-25004282 | MS.gene32024:CDS |
| ACAATTACAACAATGGTGAG+AGG | 0.652438 | 4.3:-25004317 | MS.gene32024:CDS |
| AGTGACACAAGAGTTATGGA+AGG | 0.692016 | 4.3:-25004285 | MS.gene32024:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAAACCATAACTGTTTT+CGG | + | chr4.3:25004264-25004283 | None:intergenic | 20.0% |
| ! | AAATACAATCCAACATTCAA+TGG | - | chr4.3:25004956-25004975 | MS.gene32024:CDS | 25.0% |
| ! | AATTTGGAGAGAAAATGAAA+GGG | + | chr4.3:25004114-25004133 | None:intergenic | 25.0% |
| ! | AGTAACAACAATTACAACAA+TGG | - | chr4.3:25004860-25004879 | MS.gene32024:CDS | 25.0% |
| ! | GAAAACAGTTATGGTTTATA+TGG | - | chr4.3:25004263-25004282 | MS.gene32024:CDS | 25.0% |
| ! | TACAACAAAAAAGGTTACTT+TGG | - | chr4.3:25005016-25005035 | MS.gene32024:CDS | 25.0% |
| ! | TTGTTAAGAATAGGAAAGAA+AGG | + | chr4.3:25004091-25004110 | None:intergenic | 25.0% |
| ! | TTTAATGGTGTTTCATTGTT+AGG | + | chr4.3:25004206-25004225 | None:intergenic | 25.0% |
| !! | TTTTTATGATGTTAACTCTG+AGG | - | chr4.3:25004931-25004950 | MS.gene32024:CDS | 25.0% |
| !!! | AAAGAAAGGAATTTTGAGAT+GGG | + | chr4.3:25004077-25004096 | None:intergenic | 25.0% |
| !!! | ACGAAGTTTTTTCATATGAT+TGG | + | chr4.3:25004352-25004371 | None:intergenic | 25.0% |
| AATAACAAGAACTCCATGAA+TGG | - | chr4.3:25005064-25005083 | MS.gene32024:CDS | 30.0% | |
| ATGAAAGGGTTGTTAAGAAT+AGG | + | chr4.3:25004100-25004119 | None:intergenic | 30.0% | |
| GAATTTGGAGAGAAAATGAA+AGG | + | chr4.3:25004115-25004134 | None:intergenic | 30.0% | |
| GAGAATTGGTACAACAAAAA+AGG | - | chr4.3:25005007-25005026 | MS.gene32024:CDS | 30.0% | |
| TTCAATGGAGATTCATCTAA+AGG | - | chr4.3:25004971-25004990 | MS.gene32024:CDS | 30.0% | |
| TTGTTTGTGTTAGTAGTACT+AGG | + | chr4.3:25004311-25004330 | None:intergenic | 30.0% | |
| TTTCTACAACAACAACAACA+AGG | - | chr4.3:25004622-25004641 | MS.gene32024:CDS | 30.0% | |
| ! | ATTGTTGTTGTTGTTGACAT+GGG | + | chr4.3:25004171-25004190 | None:intergenic | 30.0% |
| ! | TTCATATGATTGGAAAGTGT+TGG | + | chr4.3:25004342-25004361 | None:intergenic | 30.0% |
| !! | AGAAATACTTGTTGTTGTTC+TGG | + | chr4.3:25004700-25004719 | None:intergenic | 30.0% |
| !! | CAAGTATTCTTCTAGTTCTA+AGG | - | chr4.3:25004520-25004539 | MS.gene32024:CDS | 30.0% |
| !! | GAAAGAAAGGAATTTTGAGA+TGG | + | chr4.3:25004078-25004097 | None:intergenic | 30.0% |
| !! | TCATATGATTGGAAAGTGTT+GGG | + | chr4.3:25004341-25004360 | None:intergenic | 30.0% |
| !! | TGTTGTTCTGGTTTGTTTAA+TGG | + | chr4.3:25004221-25004240 | None:intergenic | 30.0% |
| AACAAAAAGTTCCCAGAAGA+AGG | - | chr4.3:25004551-25004570 | MS.gene32024:CDS | 35.0% | |
| AACGCTAAGTTTACTGAAGA+AGG | - | chr4.3:25004656-25004675 | MS.gene32024:CDS | 35.0% | |
| AATGAGTGACACAAGAGTTA+TGG | - | chr4.3:25004895-25004914 | MS.gene32024:CDS | 35.0% | |
| ACAATTACAACAATGGTGAG+AGG | - | chr4.3:25004867-25004886 | MS.gene32024:CDS | 35.0% | |
| AGTTAGTGACACCAAACTAA+CGG | - | chr4.3:25004454-25004473 | MS.gene32024:CDS | 35.0% | |
| GATGAATCTCCATTGAATGT+TGG | + | chr4.3:25004968-25004987 | None:intergenic | 35.0% | |
| GGTTTATATGGTCATGATAC+TGG | - | chr4.3:25004275-25004294 | MS.gene32024:CDS | 35.0% | |
| TCGAGTTATATCCTTCTTCT+GGG | + | chr4.3:25004565-25004584 | None:intergenic | 35.0% | |
| TGAAGAAGGATACAAATCCA+TGG | - | chr4.3:25004670-25004689 | MS.gene32024:CDS | 35.0% | |
| TGTTGTTATAGGAATCTTGC+TGG | + | chr4.3:25004421-25004440 | None:intergenic | 35.0% | |
| TTGATTCTGGTAACCATTCA+TGG | + | chr4.3:25005080-25005099 | None:intergenic | 35.0% | |
| TTGTGTTAGTAGTACTAGGA+GGG | + | chr4.3:25004307-25004326 | None:intergenic | 35.0% | |
| TTTGTGTTAGTAGTACTAGG+AGG | + | chr4.3:25004308-25004327 | None:intergenic | 35.0% | |
| ! | ATGAAAACTGGTTGTTGTTC+TGG | + | chr4.3:25004233-25004252 | None:intergenic | 35.0% |
| ! | ATTGAGTTGTAACCTTCTTC+TGG | + | chr4.3:25004758-25004777 | None:intergenic | 35.0% |
| ! | CATTGTTGTTGTTGTTGACA+TGG | + | chr4.3:25004172-25004191 | None:intergenic | 35.0% |
| ! | GTAGTTGTAGTTGTTGTTGT+TGG | + | chr4.3:25004492-25004511 | None:intergenic | 35.0% |
| ! | TTCGAGTTATATCCTTCTTC+TGG | + | chr4.3:25004566-25004585 | None:intergenic | 35.0% |
| ! | TTGAGTTGTAACCTTCTTCT+GGG | + | chr4.3:25004757-25004776 | None:intergenic | 35.0% |
| !! | ATTCGGGTACTTGTTGTTAT+AGG | + | chr4.3:25004432-25004451 | None:intergenic | 35.0% |
| !! | TTGGTGTCACTAACTTCATT+CGG | + | chr4.3:25004449-25004468 | None:intergenic | 35.0% |
| AACACAAAGTTCCCAGAAGA+AGG | - | chr4.3:25004743-25004762 | MS.gene32024:CDS | 40.0% | |
| AGTGACACAAGAGTTATGGA+AGG | - | chr4.3:25004899-25004918 | MS.gene32024:CDS | 40.0% | |
| CTTCAAACTGCTCTTGATTC+TGG | + | chr4.3:25005093-25005112 | None:intergenic | 40.0% | |
| GTAAGATGTTCCCGTTAGTT+TGG | + | chr4.3:25004468-25004487 | None:intergenic | 40.0% | |
| GTTAGTGACACCAAACTAAC+GGG | - | chr4.3:25004455-25004474 | MS.gene32024:CDS | 40.0% | |
| ! | ACAACAACCAGTTTTCATCC+CGG | - | chr4.3:25004235-25004254 | MS.gene32024:CDS | 40.0% |
| ! | CATAACTGTTTTCGGTTTCC+GGG | + | chr4.3:25004256-25004275 | None:intergenic | 40.0% |
| ! | CCATAACTGTTTTCGGTTTC+CGG | + | chr4.3:25004257-25004276 | None:intergenic | 40.0% |
| ! | CGTAGTTGTTGTTGTTGTTC+TGG | + | chr4.3:25004787-25004806 | None:intergenic | 40.0% |
| ! | GAGTTGTGAACTCTGAGAAT+TGG | - | chr4.3:25004993-25005012 | MS.gene32024:CDS | 40.0% |
| !! | TGGTGTCACTAACTTCATTC+GGG | + | chr4.3:25004448-25004467 | None:intergenic | 40.0% |
| !!! | GTTGTTGTTCTGGTTTTCCA+TGG | + | chr4.3:25004690-25004709 | None:intergenic | 40.0% |
| CCGGAAACCGAAAACAGTTA+TGG | - | chr4.3:25004254-25004273 | MS.gene32024:CDS | 45.0% | |
| GACACAAGAGTTATGGAAGG+TGG | - | chr4.3:25004902-25004921 | MS.gene32024:CDS | 45.0% | |
| GACTGTCTCTGGCATGAATT+TGG | + | chr4.3:25004130-25004149 | None:intergenic | 45.0% | |
| GCTGAAGAATTGACTGTCTC+TGG | + | chr4.3:25004141-25004160 | None:intergenic | 45.0% | |
| TACAACAATGGTGAGAGGCA+AGG | - | chr4.3:25004872-25004891 | MS.gene32024:CDS | 45.0% | |
| TCGGTTTCCGGGATGAAAAC+TGG | + | chr4.3:25004245-25004264 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 25004068 | 25005138 | 25004068 | ID=MS.gene32024 |
| chr4.3 | mRNA | 25004068 | 25005138 | 25004068 | ID=MS.gene32024.t1;Parent=MS.gene32024 |
| chr4.3 | exon | 25004068 | 25005138 | 25004068 | ID=MS.gene32024.t1.exon1;Parent=MS.gene32024.t1 |
| chr4.3 | CDS | 25004068 | 25005138 | 25004068 | ID=cds.MS.gene32024.t1;Parent=MS.gene32024.t1 |
| Gene Sequence |
| Protein sequence |
