AlfalfaGEDB | MS.gene069962

M. sativa cultivar XinJiangDaYe / MS.gene069962

KEGG: K02966
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01090.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069962.t1	XP_004516579.1	98.9	91	1	0	1	91	29	119	1.80E-45	191.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069962.t1	Q9SGA6	83.5	91	15	0	1	91	29	119	2.7e-42	172.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069962.t1	A0A1S2Z7S6	98.9	91	1	0	1	91	29	119	1.3e-45	191.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049140	MS.gene069962	0.817359	3.44E-52	-1.69E-46
MS.gene049411	MS.gene069962	0.802367	5.80E-49	-1.69E-46
MS.gene050277	MS.gene069962	0.876575	1.25E-68	-1.69E-46
MS.gene050290	MS.gene069962	0.854339	1.31E-61	-1.69E-46
MS.gene050320	MS.gene069962	0.861362	1.08E-63	-1.69E-46
MS.gene050752	MS.gene069962	0.861838	7.74E-64	-1.69E-46
MS.gene050838	MS.gene069962	0.867124	1.72E-65	-1.69E-46
MS.gene050916	MS.gene069962	0.803285	3.75E-49	-1.69E-46
MS.gene050955	MS.gene069962	0.849909	2.39E-60	-1.69E-46
MS.gene051003	MS.gene069962	0.803212	3.88E-49	-1.69E-46
MS.gene051063	MS.gene069962	0.837332	5.53E-57	-1.69E-46
MS.gene051234	MS.gene069962	0.812984	3.22E-51	-1.69E-46
MS.gene051330	MS.gene069962	0.828841	7.18E-55	-1.69E-46
MS.gene05134	MS.gene069962	0.801466	8.89E-49	-1.69E-46
MS.gene051451	MS.gene069962	0.808988	2.36E-50	-1.69E-46
MS.gene051746	MS.gene069962	0.908203	2.21E-81	-1.69E-46
MS.gene051748	MS.gene069962	0.905715	3.20E-80	-1.69E-46
MS.gene051756	MS.gene069962	0.85117	1.06E-60	-1.69E-46
MS.gene051850	MS.gene069962	0.872619	2.77E-67	-1.69E-46
MS.gene051903	MS.gene069962	0.938293	8.55E-99	-1.69E-46
MS.gene052479	MS.gene069962	0.810333	1.21E-50	-1.69E-46
MS.gene052947	MS.gene069962	0.884538	1.76E-71	-1.69E-46
MS.gene053109	MS.gene069962	0.852211	5.36E-61	-1.69E-46
MS.gene053952	MS.gene069962	0.89573	7.26E-76	-1.69E-46
MS.gene054403	MS.gene069962	0.861762	8.17E-64	-1.69E-46
MS.gene054937	MS.gene069962	0.902183	1.26E-78	-1.69E-46
MS.gene055162	MS.gene069962	0.835191	1.94E-56	-1.69E-46
MS.gene055424	MS.gene069962	0.832364	9.86E-56	-1.69E-46
MS.gene055555	MS.gene069962	0.815024	1.14E-51	-1.69E-46
MS.gene056075	MS.gene069962	0.88672	2.68E-72	-1.69E-46
MS.gene056601	MS.gene069962	0.840679	7.52E-58	-1.69E-46
MS.gene056631	MS.gene069962	0.864526	1.14E-64	-1.69E-46
MS.gene057057	MS.gene069962	0.814134	1.80E-51	-1.69E-46
MS.gene057094	MS.gene069962	0.897202	1.77E-76	-1.69E-46
MS.gene057130	MS.gene069962	0.829109	6.19E-55	-1.69E-46
MS.gene057206	MS.gene069962	0.81005	1.40E-50	-1.69E-46
MS.gene057234	MS.gene069962	0.867296	1.52E-65	-1.69E-46
MS.gene057705	MS.gene069962	0.843098	1.73E-58	-1.69E-46
MS.gene057999	MS.gene069962	0.82479	6.67E-54	-1.69E-46
MS.gene058055	MS.gene069962	0.818696	1.71E-52	-1.69E-46
MS.gene058074	MS.gene069962	0.816845	4.48E-52	-1.69E-46
MS.gene058238	MS.gene069962	0.83468	2.61E-56	-1.69E-46
MS.gene058245	MS.gene069962	0.81145	6.96E-51	-1.69E-46
MS.gene058906	MS.gene069962	0.826207	3.08E-54	-1.69E-46
MS.gene059227	MS.gene069962	0.807957	3.92E-50	-1.69E-46
MS.gene059726	MS.gene069962	0.8493	3.53E-60	-1.69E-46
MS.gene059898	MS.gene069962	0.848183	7.21E-60	-1.69E-46
MS.gene06001	MS.gene069962	0.893845	4.30E-75	-1.69E-46
MS.gene06011	MS.gene069962	0.827529	1.49E-54	-1.69E-46
MS.gene060763	MS.gene069962	0.822415	2.40E-53	-1.69E-46
MS.gene060856	MS.gene069962	0.835124	2.01E-56	-1.69E-46
MS.gene060917	MS.gene069962	0.801969	7.01E-49	-1.69E-46
MS.gene061145	MS.gene069962	0.862397	5.22E-64	-1.69E-46
MS.gene06118	MS.gene069962	0.887601	1.24E-72	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069962.t1	MTR_3g013635	98.901	91	1	1	91	29	119	4.79e-66	195
MS.gene069962.t1	MTR_3g013635	98.901	91	1	1	91	29	119	4.79e-66	195
MS.gene069962.t1	MTR_8g040740	92.308	91	7	1	91	29	119	2.04e-63	188

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069962.t1	AT3G02080	83.516	91	15	1	91	29	119	3.79e-58	175
MS.gene069962.t1	AT5G15520	84.615	91	14	1	91	29	119	7.64e-58	174
MS.gene069962.t1	AT5G61170	82.418	91	16	1	91	29	119	7.72e-58	174

Find 34 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGAAGACTGCAAGGTTTA+AGG	0.181111	3.2:+6732572	MS.gene069962:CDS
GGTGCATTCCAGAGGATTTA+TGG	0.279212	3.2:+6732810	MS.gene069962:CDS
TTCACAATATCTGTCCATTC+TGG	0.341460	3.2:-6732555	None:intergenic
CCTTGGTGTCCATCTCAATA+AGG	0.385015	3.2:-6732926	None:intergenic
GACTGCAAGGTTTAAGGAGT+TGG	0.416477	3.2:+6732578	MS.gene069962:CDS
ACCCCTCAAGTAGATCTTCC+TGG	0.431786	3.2:-6732775	None:intergenic
GACTTCCACCATAAATCCTC+TGG	0.453242	3.2:-6732818	None:intergenic
ATCTACTTGAGGGGTGGACT+CGG	0.454423	3.2:+6732783	MS.gene069962:CDS
GTGCCGCAGCTTCCATGGCC+AGG	0.485644	3.2:+6732757	MS.gene069962:intron
GCTCTGACGTAGTACCAGTC+AGG	0.489319	3.2:-6732612	None:intergenic
TAGTACCAGTCAGGGTCATA+AGG	0.498306	3.2:-6732603	None:intergenic
TATGGTGGAAGTCAGAGAAA+TGG	0.514768	3.2:+6732828	MS.gene069962:CDS
CAAGTAGATCTTCCTGGCCA+TGG	0.517027	3.2:-6732769	None:intergenic
GGCCAGGAAGATCTACTTGA+GGG	0.518439	3.2:+6732773	MS.gene069962:CDS
TTGAGGGGTGGACTCGGTGT+TGG	0.522669	3.2:+6732789	MS.gene069962:CDS
TGGCTCCTTATGACCCTGAC+TGG	0.535373	3.2:+6732598	MS.gene069962:CDS
CCACTGCTCTTGCAGAAATG+AGG	0.542089	3.2:-6732861	None:intergenic
GACTGGTACTACGTCAGAGC+TGG	0.543992	3.2:+6732615	MS.gene069962:CDS
AACAGATGGAGCTTCCAGAA+TGG	0.551656	3.2:+6732541	None:intergenic
TTGAAGAATGTGGCGAGCAA+TGG	0.555153	3.2:-6732886	None:intergenic
GAACATGAACCTTATTGAGA+TGG	0.561257	3.2:+6732917	MS.gene069962:CDS
CCTCATTTCTGCAAGAGCAG+TGG	0.589477	3.2:+6732861	MS.gene069962:CDS
TCGGTGTTGGTGCATTCCAG+AGG	0.603387	3.2:+6732802	MS.gene069962:CDS
CAGATATTGTGAAGACTGCA+AGG	0.617924	3.2:+6732565	MS.gene069962:CDS
CTGCTCTTGCAGAAATGAGG+TGG	0.621206	3.2:-6732858	None:intergenic
AGGAAGATCTACTTGAGGGG+TGG	0.621735	3.2:+6732777	MS.gene069962:CDS
CTCTGACGTAGTACCAGTCA+GGG	0.627550	3.2:-6732611	None:intergenic
CTTATTGAGATGGACACCAA+GGG	0.649426	3.2:+6732927	MS.gene069962:CDS
TGGCCAGGAAGATCTACTTG+AGG	0.650322	3.2:+6732772	MS.gene069962:CDS
CCTTATTGAGATGGACACCA+AGG	0.671459	3.2:+6732926	MS.gene069962:CDS
GCATTCCAGAGGATTTATGG+TGG	0.673361	3.2:+6732813	MS.gene069962:CDS
TCTGCAACTGTTGAAGAATG+TGG	0.679137	3.2:-6732896	None:intergenic
GCCAGGAAGATCTACTTGAG+GGG	0.686642	3.2:+6732774	MS.gene069962:CDS
TTATTGAGATGGACACCAAG+GGG	0.704884	3.2:+6732928	MS.gene069962:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTGTTAATTTAAACAA+GGG	-	chr3.2:6732677-6732696	None:intergenic	10.0%
!!!	TTTTTTTGTTAATTTAAACA+AGG	-	chr3.2:6732678-6732697	None:intergenic	10.0%
!!	AACAAAAAAATAACTTTCGA+GGG	+	chr3.2:6732688-6732707	MS.gene069962:intron	20.0%
!!	TAACAAAAAAATAACTTTCG+AGG	+	chr3.2:6732687-6732706	MS.gene069962:intron	20.0%
!	ACATTGCAATTTTCAACAAC+AGG	-	chr3.2:6732730-6732749	None:intergenic	30.0%
	GAACATGAACCTTATTGAGA+TGG	+	chr3.2:6732917-6732936	MS.gene069962:CDS	35.0%
	GTTAATTTAAACAAGGGACC+AGG	-	chr3.2:6732671-6732690	None:intergenic	35.0%
	TTCACAATATCTGTCCATTC+TGG	-	chr3.2:6732558-6732577	None:intergenic	35.0%
!!	GTTTGATTTTGTGCATTTCC+TGG	+	chr3.2:6732650-6732669	MS.gene069962:intron	35.0%
	CAGATATTGTGAAGACTGCA+AGG	+	chr3.2:6732565-6732584	MS.gene069962:CDS	40.0%
	CTTATTGAGATGGACACCAA+GGG	+	chr3.2:6732927-6732946	MS.gene069962:CDS	40.0%
	TCTGCAACTGTTGAAGAATG+TGG	-	chr3.2:6732899-6732918	None:intergenic	40.0%
	TGTGAAGACTGCAAGGTTTA+AGG	+	chr3.2:6732572-6732591	MS.gene069962:CDS	40.0%
!	TATGGTGGAAGTCAGAGAAA+TGG	+	chr3.2:6732828-6732847	MS.gene069962:CDS	40.0%
	CCTTATTGAGATGGACACCA+AGG	+	chr3.2:6732926-6732945	MS.gene069962:CDS	45.0%
	GACTGCAAGGTTTAAGGAGT+TGG	+	chr3.2:6732578-6732597	MS.gene069962:CDS	45.0%
	GACTTCCACCATAAATCCTC+TGG	-	chr3.2:6732821-6732840	None:intergenic	45.0%
	GCATTCCAGAGGATTTATGG+TGG	+	chr3.2:6732813-6732832	MS.gene069962:CDS	45.0%
	GGTGCATTCCAGAGGATTTA+TGG	+	chr3.2:6732810-6732829	MS.gene069962:CDS	45.0%
	TAGTACCAGTCAGGGTCATA+AGG	-	chr3.2:6732606-6732625	None:intergenic	45.0%
	TTGAAGAATGTGGCGAGCAA+TGG	-	chr3.2:6732889-6732908	None:intergenic	45.0%
!!	CCTTGGTGTCCATCTCAATA+AGG	-	chr3.2:6732929-6732948	None:intergenic	45.0%
	ACCCCTCAAGTAGATCTTCC+TGG	-	chr3.2:6732778-6732797	None:intergenic	50.0%
	AGGAAGATCTACTTGAGGGG+TGG	+	chr3.2:6732777-6732796	MS.gene069962:CDS	50.0%
	CAAGTAGATCTTCCTGGCCA+TGG	-	chr3.2:6732772-6732791	None:intergenic	50.0%
	CCACTGCTCTTGCAGAAATG+AGG	-	chr3.2:6732864-6732883	None:intergenic	50.0%
	CCTCATTTCTGCAAGAGCAG+TGG	+	chr3.2:6732861-6732880	MS.gene069962:CDS	50.0%
	CTCTGACGTAGTACCAGTCA+GGG	-	chr3.2:6732614-6732633	None:intergenic	50.0%
	CTGCTCTTGCAGAAATGAGG+TGG	-	chr3.2:6732861-6732880	None:intergenic	50.0%
	GCCAGGAAGATCTACTTGAG+GGG	+	chr3.2:6732774-6732793	MS.gene069962:CDS	50.0%
	GGCCAGGAAGATCTACTTGA+GGG	+	chr3.2:6732773-6732792	MS.gene069962:CDS	50.0%
	TGGCCAGGAAGATCTACTTG+AGG	+	chr3.2:6732772-6732791	MS.gene069962:CDS	50.0%
!	ATCTACTTGAGGGGTGGACT+CGG	+	chr3.2:6732783-6732802	MS.gene069962:CDS	50.0%
!	TTTTTGTGCCGCAGCTTCCA+TGG	+	chr3.2:6732752-6732771	MS.gene069962:intron	50.0%
	GACTGGTACTACGTCAGAGC+TGG	+	chr3.2:6732615-6732634	MS.gene069962:CDS	55.0%
	GCTCTGACGTAGTACCAGTC+AGG	-	chr3.2:6732615-6732634	None:intergenic	55.0%
	TGGCTCCTTATGACCCTGAC+TGG	+	chr3.2:6732598-6732617	MS.gene069962:CDS	55.0%
!!	TCGGTGTTGGTGCATTCCAG+AGG	+	chr3.2:6732802-6732821	MS.gene069962:CDS	55.0%
	CTTCCTGGCCATGGAAGCTG+CGG	-	chr3.2:6732763-6732782	None:intergenic	60.0%
!!	TTGAGGGGTGGACTCGGTGT+TGG	+	chr3.2:6732789-6732808	MS.gene069962:CDS	60.0%
	GTGCCGCAGCTTCCATGGCC+AGG	+	chr3.2:6732757-6732776	MS.gene069962:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	6732546	6732949	6732546	ID=MS.gene069962
chr3.2	mRNA	6732546	6732949	6732546	ID=MS.gene069962.t1;Parent=MS.gene069962
chr3.2	exon	6732546	6732636	6732546	ID=MS.gene069962.t1.exon1;Parent=MS.gene069962.t1
chr3.2	CDS	6732546	6732636	6732546	ID=cds.MS.gene069962.t1;Parent=MS.gene069962.t1
chr3.2	exon	6732766	6732949	6732766	ID=MS.gene069962.t1.exon2;Parent=MS.gene069962.t1
chr3.2	CDS	6732766	6732949	6732766	ID=cds.MS.gene069962.t1;Parent=MS.gene069962.t1

Gene Sequence

>MS.gene069962

ATGGAGCTTCCAGAATGGACAGATATTGTGAAGACTGCAAGGTTTAAGGAGTTGGCTCCTTATGACCCTGACTGGTACTACGTCAGAGCTGGTGAGTTTCTTGTGTTTGATTTTGTGCATTTCCTGGTCCCTTGTTTAAATTAACAAAAAAATAACTTTCGAGGGAACATTTTTTGATCAACCTGTTGTTGAAAATTGCAATGTGTTTTTTGTGCCGCAGCTTCCATGGCCAGGAAGATCTACTTGAGGGGTGGACTCGGTGTTGGTGCATTCCAGAGGATTTATGGTGGAAGTCAGAGAAATGGCAGTCGTCCACCTCATTTCTGCAAGAGCAGTGGTTCCATTGCTCGCCACATTCTTCAACAGTTGCAGAACATGAACCTTATTGAGATGGACACCAAGGG

Protein sequence

>MS.gene069962.t1

MELPEWTDIVKTARFKELAPYDPDWYYVRAASMARKIYLRGGLGVGAFQRIYGGSQRNGSRPPHFCKSSGSIARHILQQLQNMNLIEMDTK