AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67371


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67371.t1 MTR_4g062520 95.710 303 10 1 1 300 1 303 0.0 574
MS.gene67371.t1 MTR_4g062470 89.216 306 25 4 1 300 1 304 6.32e-158 442
MS.gene67371.t1 MTR_1g115510 43.214 280 140 9 37 300 77 353 8.90e-63 202
MS.gene67371.t1 MTR_7g021300 44.656 262 124 9 51 299 135 388 2.75e-61 199
MS.gene67371.t1 MTR_4g062460 40.625 224 125 4 79 298 38 257 1.25e-50 168
MS.gene67371.t1 MTR_1g046800 46.018 226 112 5 75 300 35 250 6.59e-50 166
MS.gene67371.t1 MTR_1g046500 46.725 229 106 7 75 300 35 250 9.11e-48 160
MS.gene67371.t1 MTR_1g046490 45.175 228 113 7 75 300 19 236 2.34e-45 154
MS.gene67371.t1 MTR_1g115515 33.937 221 102 9 63 274 24 209 5.75e-24 97.8
MS.gene67371.t1 MTR_7g093820 37.383 107 66 1 174 279 71 177 1.00e-11 63.2
MS.gene67371.t1 MTR_7g093850 37.383 107 66 1 174 279 71 177 1.08e-11 63.2
MS.gene67371.t1 MTR_7g093830 37.383 107 66 1 174 279 70 176 2.80e-11 62.0
MS.gene67371.t1 MTR_4g013310 33.613 119 68 4 184 300 87 196 3.76e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67371.t1 AT1G07730 64.979 237 73 5 71 300 156 389 1.45e-88 270
MS.gene67371.t1 AT2G28670 65.823 237 69 5 73 300 203 436 3.42e-87 268
MS.gene67371.t1 AT2G28670 66.079 227 67 4 83 300 1 226 2.69e-86 258
MS.gene67371.t1 AT3G55230 40.328 305 160 8 14 300 3 303 1.38e-63 203
MS.gene67371.t1 AT2G39430 46.186 236 111 5 79 300 87 320 1.05e-61 198
MS.gene67371.t1 AT4G13580 41.532 248 121 8 59 300 15 244 1.65e-45 154
MS.gene67371.t1 AT3G24020 43.363 226 104 8 81 300 36 243 1.76e-43 149
MS.gene67371.t1 AT2G21100 34.677 124 73 4 155 275 49 167 2.08e-12 65.1
MS.gene67371.t1 AT2G21100 34.677 124 73 4 155 275 49 167 2.08e-12 65.1

Find 75 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGTTGTTGCGGCTGTTAT+CGG 0.213458 4.1:+37450134 None:intergenic
TGCTATTATTAAGACATTTC+CGG 0.262574 4.1:-37449508 MS.gene67371:CDS
GAAGATAGTCTCAGCTTCTT+TGG 0.282557 4.1:-37449612 MS.gene67371:CDS
TTGAGAGGCATAGTGGTAAA+AGG 0.290005 4.1:+37450299 None:intergenic
TCTCAAATCCATACTGCTTA+TGG 0.330814 4.1:-37450282 MS.gene67371:CDS
CAATCACAACTTGCAATCAT+TGG 0.336329 4.1:-37449564 MS.gene67371:CDS
TGGTTGTTGCGGCTGTTATC+GGG 0.342275 4.1:+37450135 None:intergenic
AACAATCTCCCTTTCCTAAC+AGG 0.351923 4.1:-37449903 MS.gene67371:CDS
TTGAAAGAGACCCTACTAAT+AGG 0.352692 4.1:+37449422 None:intergenic
CACTGCTCTTGCTGTTGGAT+TGG 0.363738 4.1:+37450049 None:intergenic
CTTGCAATCATTGGAGGAAC+TGG 0.372185 4.1:-37449555 MS.gene67371:CDS
TGTTTCCACTAAGTCCTGTT+AGG 0.386199 4.1:+37449889 None:intergenic
CCTTTCCTAACAGGACTTAG+TGG 0.390262 4.1:-37449894 MS.gene67371:CDS
GGAAAGTATGTGAATGCTAA+TGG 0.401273 4.1:-37449534 MS.gene67371:CDS
CCGGTCACTGCTCTTGCTGT+TGG 0.404955 4.1:+37450044 None:intergenic
AGTGGAAGGTTTGCGCCGTT+TGG 0.405131 4.1:+37449975 None:intergenic
TATCAATGCCATAAGCAGTA+TGG 0.412453 4.1:+37450274 None:intergenic
ACTGCAAAGTTTGAAGAGAA+TGG 0.412582 4.1:-37449639 MS.gene67371:CDS
CTACTTGAGCGTTGAGGGCG+GGG 0.416154 4.1:+37450009 None:intergenic
GATTCTGCTGAATCAACAAT+TGG 0.416651 4.1:+37450185 None:intergenic
GCTACTTGAGCGTTGAGGGC+GGG 0.431031 4.1:+37450008 None:intergenic
GAGTTGGGATCAGGATTAGT+TGG 0.447746 4.1:-37449723 MS.gene67371:CDS
GATAGTGTGTGGTGCTGATC+AGG 0.450730 4.1:+37450101 None:intergenic
GTTATCGGGGCGGCAAGTGG+AGG 0.450840 4.1:+37450149 None:intergenic
CACTAAGTCCTGTTAGGAAA+GGG 0.453001 4.1:+37449895 None:intergenic
ATCTGATTCATATTCGTCAC+TGG 0.455201 4.1:+37449822 None:intergenic
GAAAGCTACTTGAGCGTTGA+GGG 0.468620 4.1:+37450004 None:intergenic
GGCGGCAAGTGGAGGGAGAA+TGG 0.471632 4.1:+37450157 None:intergenic
GCTGTTATCGGGGCGGCAAG+TGG 0.475128 4.1:+37450146 None:intergenic
GAAGGTGAAGAGTTGGGATC+AGG 0.478076 4.1:-37449732 MS.gene67371:CDS
GTGAAACTTGTGTTGTTCTG+TGG 0.480336 4.1:+37449586 None:intergenic
TGTGGAATGCCGTTGTTTAG+TGG 0.484317 4.1:+37449957 None:intergenic
ACGGTGCAGGAGCTGATGTT+TGG 0.489113 4.1:-37449786 MS.gene67371:CDS
TGTTCAATAACAATAACAAT+GGG 0.500430 4.1:-37449857 MS.gene67371:CDS
CTGACAGAAGGTGAAGAGTT+GGG 0.519027 4.1:-37449738 MS.gene67371:CDS
ACTGACAGAAGGTGAAGAGT+TGG 0.521230 4.1:-37449739 MS.gene67371:CDS
ACCGCAGGGAACGACGGTGC+AGG 0.530207 4.1:-37449799 MS.gene67371:CDS
CCACTAAGTCCTGTTAGGAA+AGG 0.530721 4.1:+37449894 None:intergenic
CTGATCAGGAATATCAGTAC+TGG 0.531685 4.1:+37450115 None:intergenic
TTATCGGGGCGGCAAGTGGA+GGG 0.550525 4.1:+37450150 None:intergenic
AATAGGAAATATATGCAGAA+AGG 0.551766 4.1:+37449439 None:intergenic
ATTAAGACATTTCCGGTCAA+CGG 0.552577 4.1:-37449501 MS.gene67371:CDS
CGAAAGCTACTTGAGCGTTG+AGG 0.552792 4.1:+37450003 None:intergenic
TATATAGCAAGTTCAGTGGA+AGG 0.554582 4.1:-37449684 MS.gene67371:CDS
GTAGCTTTCGCAAAACCAAA+CGG 0.556361 4.1:-37449990 MS.gene67371:CDS
GTGTTCAATAACAATAACAA+TGG 0.566776 4.1:-37449858 MS.gene67371:CDS
GGTCAACAACATAACACTGA+TGG 0.569982 4.1:-37449480 MS.gene67371:CDS
GGATTAGTTGGAAAAGCTCA+AGG 0.573572 4.1:-37449711 MS.gene67371:CDS
TTATGTTGTTGACCGTTGAC+CGG 0.573639 4.1:+37449489 None:intergenic
AAGTTGTGGAAGTGAATGTA+AGG 0.574643 4.1:+37450246 None:intergenic
GAATATGAATCAGATACCGC+AGG 0.584877 4.1:-37449814 MS.gene67371:CDS
TATGGATTTGAGAGGCATAG+TGG 0.588428 4.1:+37450292 None:intergenic
TCTGATTCATATTCGTCACT+GGG 0.589926 4.1:+37449823 None:intergenic
CCATTGTTGTTATTGTTCTG+TGG 0.590355 4.1:+37449939 None:intergenic
GGAAGGAACAAGTCAAGTAA+TGG 0.591485 4.1:-37449667 MS.gene67371:CDS
TCACAACTTGCAATCATTGG+AGG 0.595719 4.1:-37449561 MS.gene67371:CDS
AGCTACTTGAGCGTTGAGGG+CGG 0.596968 4.1:+37450007 None:intergenic
GGTTGTTGCGGCTGTTATCG+GGG 0.598779 4.1:+37450136 None:intergenic
CCAACAGCAAGAGCAGTGAC+CGG 0.600488 4.1:-37450044 MS.gene67371:CDS
GAATGCCGTTGTTTAGTGGA+AGG 0.601191 4.1:+37449961 None:intergenic
CCACAGAACAATAACAACAA+TGG 0.609944 4.1:-37449939 MS.gene67371:CDS
AAGAATTCGAGTTGAAGTTG+TGG 0.611757 4.1:+37450232 None:intergenic
AATATCAGTACTGGTTGTTG+CGG 0.614381 4.1:+37450124 None:intergenic
GGCGGGGTTTGTAACGACTC+CGG 0.617896 4.1:+37450025 None:intergenic
ACAGGACTTAGTGGAAACAC+AGG 0.630961 4.1:-37449885 MS.gene67371:CDS
TCAGATACCGCAGGGAACGA+CGG 0.633061 4.1:-37449805 MS.gene67371:CDS
GGCAAGTGGAGGGAGAATGG+TGG 0.639076 4.1:+37450160 None:intergenic
CAGGACTTAGTGGAAACACA+GGG 0.652633 4.1:-37449884 MS.gene67371:CDS
TCCTGCACCGTCGTTCCCTG+CGG 0.661601 4.1:+37449798 None:intergenic
TTCATGCATGACATTCTAGG+CGG 0.679448 4.1:-37450074 MS.gene67371:CDS
ATAAGCAGTATGGATTTGAG+AGG 0.680583 4.1:+37450284 None:intergenic
TGTTGCGGCTGTTATCGGGG+CGG 0.685301 4.1:+37450139 None:intergenic
TTTGATGATGAACTGACAGA+AGG 0.695500 4.1:-37449750 MS.gene67371:CDS
GCAAACCTTCCACTAAACAA+CGG 0.696199 4.1:-37449966 MS.gene67371:CDS
AATATGAATCAGATACCGCA+GGG 0.700296 4.1:-37449813 MS.gene67371:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTCAATAACAATAACAAT+GGG - chr4.1:37449897-37449916 MS.gene67371:CDS 20.0%
! AATAGGAAATATATGCAGAA+AGG + chr4.1:37450318-37450337 None:intergenic 25.0%
! GTGTTCAATAACAATAACAA+TGG - chr4.1:37449896-37449915 MS.gene67371:CDS 25.0%
! TGCTATTATTAAGACATTTC+CGG - chr4.1:37450246-37450265 MS.gene67371:CDS 25.0%
!! ATTTTATATAGCAAGTTCAG+TGG - chr4.1:37450066-37450085 MS.gene67371:CDS 25.0%
! TTTTTCATGCATGACATTCT+AGG - chr4.1:37449677-37449696 MS.gene67371:CDS 30.0%
AAGTTGTGGAAGTGAATGTA+AGG + chr4.1:37449511-37449530 None:intergenic 35.0%
AATATGAATCAGATACCGCA+GGG - chr4.1:37449941-37449960 MS.gene67371:CDS 35.0%
ACTGCAAAGTTTGAAGAGAA+TGG - chr4.1:37450115-37450134 MS.gene67371:CDS 35.0%
ATAAGCAGTATGGATTTGAG+AGG + chr4.1:37449473-37449492 None:intergenic 35.0%
ATCTGATTCATATTCGTCAC+TGG + chr4.1:37449935-37449954 None:intergenic 35.0%
ATTAAGACATTTCCGGTCAA+CGG - chr4.1:37450253-37450272 MS.gene67371:CDS 35.0%
CAATCACAACTTGCAATCAT+TGG - chr4.1:37450190-37450209 MS.gene67371:CDS 35.0%
CCACAGAACAATAACAACAA+TGG - chr4.1:37449815-37449834 MS.gene67371:CDS 35.0%
GATTCTGCTGAATCAACAAT+TGG + chr4.1:37449572-37449591 None:intergenic 35.0%
TATATAGCAAGTTCAGTGGA+AGG - chr4.1:37450070-37450089 MS.gene67371:CDS 35.0%
TATCAATGCCATAAGCAGTA+TGG + chr4.1:37449483-37449502 None:intergenic 35.0%
TCTCAAATCCATACTGCTTA+TGG - chr4.1:37449472-37449491 MS.gene67371:CDS 35.0%
TCTGATTCATATTCGTCACT+GGG + chr4.1:37449934-37449953 None:intergenic 35.0%
! CCATTGTTGTTATTGTTCTG+TGG + chr4.1:37449818-37449837 None:intergenic 35.0%
! GCATGAAAAATGATAGTGTG+TGG + chr4.1:37449667-37449686 None:intergenic 35.0%
! GGAAAGTATGTGAATGCTAA+TGG - chr4.1:37450220-37450239 MS.gene67371:CDS 35.0%
!! AAGAATTCGAGTTGAAGTTG+TGG + chr4.1:37449525-37449544 None:intergenic 35.0%
!! AATATCAGTACTGGTTGTTG+CGG + chr4.1:37449633-37449652 None:intergenic 35.0%
!! TTTGATGATGAACTGACAGA+AGG - chr4.1:37450004-37450023 MS.gene67371:CDS 35.0%
AACAATCTCCCTTTCCTAAC+AGG - chr4.1:37449851-37449870 MS.gene67371:CDS 40.0%
CACTAAGTCCTGTTAGGAAA+GGG + chr4.1:37449862-37449881 None:intergenic 40.0%
CTGATCAGGAATATCAGTAC+TGG + chr4.1:37449642-37449661 None:intergenic 40.0%
GAAGATAGTCTCAGCTTCTT+TGG - chr4.1:37450142-37450161 MS.gene67371:CDS 40.0%
GAATATGAATCAGATACCGC+AGG - chr4.1:37449940-37449959 MS.gene67371:CDS 40.0%
GCAAACCTTCCACTAAACAA+CGG - chr4.1:37449788-37449807 MS.gene67371:CDS 40.0%
GGAAGGAACAAGTCAAGTAA+TGG - chr4.1:37450087-37450106 MS.gene67371:CDS 40.0%
GGTCAACAACATAACACTGA+TGG - chr4.1:37450274-37450293 MS.gene67371:CDS 40.0%
GTAGCTTTCGCAAAACCAAA+CGG - chr4.1:37449764-37449783 MS.gene67371:CDS 40.0%
TCACAACTTGCAATCATTGG+AGG - chr4.1:37450193-37450212 MS.gene67371:CDS 40.0%
TGTTTCCACTAAGTCCTGTT+AGG + chr4.1:37449868-37449887 None:intergenic 40.0%
TTATGTTGTTGACCGTTGAC+CGG + chr4.1:37450268-37450287 None:intergenic 40.0%
TTCATGCATGACATTCTAGG+CGG - chr4.1:37449680-37449699 MS.gene67371:CDS 40.0%
! GTGAAACTTGTGTTGTTCTG+TGG + chr4.1:37450171-37450190 None:intergenic 40.0%
! TATGGATTTGAGAGGCATAG+TGG + chr4.1:37449465-37449484 None:intergenic 40.0%
! TTGAGAGGCATAGTGGTAAA+AGG + chr4.1:37449458-37449477 None:intergenic 40.0%
!! GGATTAGTTGGAAAAGCTCA+AGG - chr4.1:37450043-37450062 MS.gene67371:CDS 40.0%
ACAGGACTTAGTGGAAACAC+AGG - chr4.1:37449869-37449888 MS.gene67371:CDS 45.0%
ACTGACAGAAGGTGAAGAGT+TGG - chr4.1:37450015-37450034 MS.gene67371:CDS 45.0%
CAGGACTTAGTGGAAACACA+GGG - chr4.1:37449870-37449889 MS.gene67371:CDS 45.0%
CCACTAAGTCCTGTTAGGAA+AGG + chr4.1:37449863-37449882 None:intergenic 45.0%
CCTTTCCTAACAGGACTTAG+TGG - chr4.1:37449860-37449879 MS.gene67371:CDS 45.0%
CTGACAGAAGGTGAAGAGTT+GGG - chr4.1:37450016-37450035 MS.gene67371:CDS 45.0%
CTTGCAATCATTGGAGGAAC+TGG - chr4.1:37450199-37450218 MS.gene67371:CDS 45.0%
GAATGCCGTTGTTTAGTGGA+AGG + chr4.1:37449796-37449815 None:intergenic 45.0%
TGTGGAATGCCGTTGTTTAG+TGG + chr4.1:37449800-37449819 None:intergenic 45.0%
! GAGTTGGGATCAGGATTAGT+TGG - chr4.1:37450031-37450050 MS.gene67371:CDS 45.0%
!! GAAAGCTACTTGAGCGTTGA+GGG + chr4.1:37449753-37449772 None:intergenic 45.0%
CTGGTTGTTGCGGCTGTTAT+CGG + chr4.1:37449623-37449642 None:intergenic 50.0%
GAAGGTGAAGAGTTGGGATC+AGG - chr4.1:37450022-37450041 MS.gene67371:CDS 50.0%
TGGTTGTTGCGGCTGTTATC+GGG + chr4.1:37449622-37449641 None:intergenic 50.0%
! CGAAAGCTACTTGAGCGTTG+AGG + chr4.1:37449754-37449773 None:intergenic 50.0%
!! CACTGCTCTTGCTGTTGGAT+TGG + chr4.1:37449708-37449727 None:intergenic 50.0%
!! GATAGTGTGTGGTGCTGATC+AGG + chr4.1:37449656-37449675 None:intergenic 50.0%
ACGGTGCAGGAGCTGATGTT+TGG - chr4.1:37449968-37449987 MS.gene67371:CDS 55.0%
AGTGGAAGGTTTGCGCCGTT+TGG + chr4.1:37449782-37449801 None:intergenic 55.0%
CCAACAGCAAGAGCAGTGAC+CGG - chr4.1:37449710-37449729 MS.gene67371:CDS 55.0%
GGTTGTTGCGGCTGTTATCG+GGG + chr4.1:37449621-37449640 None:intergenic 55.0%
TCAGATACCGCAGGGAACGA+CGG - chr4.1:37449949-37449968 MS.gene67371:CDS 55.0%
!! AGCTACTTGAGCGTTGAGGG+CGG + chr4.1:37449750-37449769 None:intergenic 55.0%
GGCAAGTGGAGGGAGAATGG+TGG + chr4.1:37449597-37449616 None:intergenic 60.0%
GGCGGGGTTTGTAACGACTC+CGG + chr4.1:37449732-37449751 None:intergenic 60.0%
TGTTGCGGCTGTTATCGGGG+CGG + chr4.1:37449618-37449637 None:intergenic 60.0%
TTATCGGGGCGGCAAGTGGA+GGG + chr4.1:37449607-37449626 None:intergenic 60.0%
! CCGGTCACTGCTCTTGCTGT+TGG + chr4.1:37449713-37449732 None:intergenic 60.0%
!! CTACTTGAGCGTTGAGGGCG+GGG + chr4.1:37449748-37449767 None:intergenic 60.0%
!! GCTACTTGAGCGTTGAGGGC+GGG + chr4.1:37449749-37449768 None:intergenic 60.0%
GCTGTTATCGGGGCGGCAAG+TGG + chr4.1:37449611-37449630 None:intergenic 65.0%
GGCGGCAAGTGGAGGGAGAA+TGG + chr4.1:37449600-37449619 None:intergenic 65.0%
GTTATCGGGGCGGCAAGTGG+AGG + chr4.1:37449608-37449627 None:intergenic 65.0%
TCCTGCACCGTCGTTCCCTG+CGG + chr4.1:37449959-37449978 None:intergenic 65.0%
ACCGCAGGGAACGACGGTGC+AGG - chr4.1:37449955-37449974 MS.gene67371:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 37449437 37450339 37449437 ID=MS.gene67371
chr4.1 mRNA 37449437 37450339 37449437 ID=MS.gene67371.t1;Parent=MS.gene67371
chr4.1 exon 37449437 37450339 37449437 ID=MS.gene67371.t1.exon1;Parent=MS.gene67371.t1
chr4.1 CDS 37449437 37450339 37449437 ID=cds.MS.gene67371.t1;Parent=MS.gene67371.t1
Gene Sequence

>MS.gene67371

ATGGCACGCTTTGAAACTCCTTTTACCACTATGCCTCTCAAATCCATACTGCTTATGGCATTGATATTATTCCTTACATTCACTTCCACAACTTCAACTCGAATTCTTAGTGAACAAGAAACACTAGAAGAACCAATTGTTGATTCAGCAGAATCTTCCACCATTCTCCCTCCACTTGCCGCCCCGATAACAGCCGCAACAACCAGTACTGATATTCCTGATCAGCACCACACACTATCATTTTTCATGCATGACATTCTAGGCGGCTCCAATCCAACAGCAAGAGCAGTGACCGGAGTCGTTACAAACCCCGCCCTCAACGCTCAAGTAGCTTTCGCAAAACCAAACGGCGCAAACCTTCCACTAAACAACGGCATTCCACAGAACAATAACAACAATGGAATTCTAAACAACAACAATCTCCCTTTCCTAACAGGACTTAGTGGAAACACAGGGAATGTGTTCAATAACAATAACAATGGGAACAACAATTTCCCAGTGACGAATATGAATCAGATACCGCAGGGAACGACGGTGCAGGAGCTGATGTTTGGTACAATGACAGTGTTTGATGATGAACTGACAGAAGGTGAAGAGTTGGGATCAGGATTAGTTGGAAAAGCTCAAGGATTTTATATAGCAAGTTCAGTGGAAGGAACAAGTCAAGTAATGGCATTCACTGCAAAGTTTGAAGAGAATGGTTATGAAGATAGTCTCAGCTTCTTTGGAGTCCACAGAACAACACAAGTTTCACAATCACAACTTGCAATCATTGGAGGAACTGGAAAGTATGTGAATGCTAATGGATTTGCTATTATTAAGACATTTCCGGTCAACGGTCAACAACATAACACTGATGGACTTGAAACTCTCTTGCACCTTTCTGCATATATTTCCTATTAG

Protein sequence

>MS.gene67371.t1

MARFETPFTTMPLKSILLMALILFLTFTSTTSTRILSEQETLEEPIVDSAESSTILPPLAAPITAATTSTDIPDQHHTLSFFMHDILGGSNPTARAVTGVVTNPALNAQVAFAKPNGANLPLNNGIPQNNNNNGILNNNNLPFLTGLSGNTGNVFNNNNNGNNNFPVTNMNQIPQGTTVQELMFGTMTVFDDELTEGEELGSGLVGKAQGFYIASSVEGTSQVMAFTAKFEENGYEDSLSFFGVHRTTQVSQSQLAIIGGTGKYVNANGFAIIKTFPVNGQQHNTDGLETLLHLSAYISY