AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81770.t1 MTR_1g058570 96.907 194 6 0 1 194 1 194 2.54e-136 379
MS.gene81770.t1 MTR_1g058540 79.082 196 39 1 1 194 1 196 1.00e-100 289
MS.gene81770.t1 MTR_1g058560 86.667 195 25 1 1 194 1 195 2.06e-98 283
MS.gene81770.t1 MTR_2g086740 56.818 176 76 0 18 193 44 219 3.14e-57 180
MS.gene81770.t1 MTR_7g011090 49.180 183 89 1 12 194 8 186 5.78e-55 173
MS.gene81770.t1 MTR_4g081880 43.243 185 100 2 9 193 7 186 3.53e-48 155
MS.gene81770.t1 MTR_5g041900 46.108 167 90 0 28 194 20 186 9.49e-43 142
MS.gene81770.t1 MTR_2g086740 57.944 107 45 0 18 124 44 150 2.28e-32 114
MS.gene81770.t1 MTR_0004s0210 35.849 159 89 4 31 187 3 150 2.30e-18 78.6
MS.gene81770.t1 MTR_7g108130 28.049 164 113 3 31 191 24 185 8.39e-14 67.0
MS.gene81770.t1 MTR_0097s0070 31.356 118 79 2 71 187 69 185 8.61e-13 64.3
MS.gene81770.t1 MTR_8g089300 31.013 158 96 4 40 187 30 184 9.54e-12 61.6
MS.gene81770.t1 MTR_3g064310 32.203 118 77 2 74 191 77 191 4.51e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81770.t1 AT2G36100 58.911 202 74 1 2 194 5 206 7.78e-73 219
MS.gene81770.t1 AT5G06200 61.421 197 71 2 2 193 5 201 2.20e-63 195
MS.gene81770.t1 AT3G11550 60.500 200 72 2 2 194 5 204 4.66e-62 191
MS.gene81770.t1 AT2G27370 58.929 168 69 0 26 193 52 219 1.00e-60 189
MS.gene81770.t1 AT5G15290 48.000 150 78 0 25 174 17 166 1.22e-47 154
MS.gene81770.t1 AT1G14160 40.606 165 98 0 30 194 45 209 1.43e-41 139
MS.gene81770.t1 AT1G03700 32.215 149 92 2 39 187 12 151 6.87e-19 80.1
MS.gene81770.t1 AT4G03540 33.058 121 72 2 55 175 28 139 2.96e-15 70.5
MS.gene81770.t1 AT1G17200 27.545 167 109 4 29 191 23 181 2.95e-14 68.6
MS.gene81770.t1 AT4G15630 28.492 179 108 5 25 191 19 189 5.96e-14 67.4
MS.gene81770.t1 AT5G44550 30.864 162 100 4 40 193 22 179 2.24e-12 63.2
MS.gene81770.t1 AT3G06390 28.571 133 90 2 36 163 37 169 3.62e-12 62.8

Find 44 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTAATGTTGCTTGGTTC+TGG 0.115217 1.3:+43261246 None:intergenic
CTAGCCATTTGCAACCAATT+TGG 0.242258 1.3:-43260557 MS.gene81770:CDS
GTGATGTCTGCTTTGGCTCT+TGG 0.283856 1.3:-43260461 MS.gene81770:CDS
AAGGCCTGGAGGATGGAAAA+AGG 0.302153 1.3:-43261196 MS.gene81770:CDS
CCTTAGAATGAAATCCATTA+TGG 0.309686 1.3:+43261167 None:intergenic
CCATAATGGATTTCATTCTA+AGG 0.312115 1.3:-43261167 MS.gene81770:CDS
TTCCATCCTCCAGGCCTTGT+AGG 0.319831 1.3:+43261201 None:intergenic
TTACGATATCTAAGATAATA+AGG 0.319981 1.3:+43260834 None:intergenic
TGATGTCTGCTTTGGCTCTT+GGG 0.345325 1.3:-43260460 MS.gene81770:CDS
TATTGCCGCTGCCGCCTCAA+TGG 0.357738 1.3:-43261124 MS.gene81770:CDS
TGTTAATGTTGCTTGGTTCT+GGG 0.362737 1.3:+43261247 None:intergenic
ATTGCCGCTGCCGCCTCAAT+GGG 0.364904 1.3:-43261123 MS.gene81770:CDS
ATGGATTTCATTCTAAGGTT+AGG 0.379588 1.3:-43261162 MS.gene81770:CDS
AGCTGCTTCCATAGTTTACT+TGG 0.387801 1.3:-43260609 MS.gene81770:CDS
GTTGGTTGTGATGTCTGCTT+TGG 0.390211 1.3:-43260468 MS.gene81770:CDS
GTGCACAGAAATCTCCAAAT+TGG 0.409853 1.3:+43260543 None:intergenic
ATGGAAATCAAGACACAAAC+TGG 0.414877 1.3:-43260580 MS.gene81770:CDS
AGCTGCAGCAGCACTTGAAG+TGG 0.421850 1.3:+43260628 None:intergenic
AAGCCTTGGTCCAGGCGCAT+GGG 0.441787 1.3:+43260859 None:intergenic
AAAGCCTTGGTCCAGGCGCA+TGG 0.443522 1.3:+43260858 None:intergenic
AGCGGCAATAGCCGCTGCTA+TGG 0.444367 1.3:+43261137 None:intergenic
ATCTCCAAATTGGTTGCAAA+TGG 0.445100 1.3:+43260553 None:intergenic
GCTTGGTTCTGGGACATCAA+TGG 0.457904 1.3:+43261257 None:intergenic
TGGTAGGGATAGAACTAAGT+AGG 0.473587 1.3:+43260907 None:intergenic
TGTTCCCATTGAGGCGGCAG+CGG 0.482406 1.3:+43261119 None:intergenic
CTAGTTGCACCTACAAGGCC+TGG 0.489392 1.3:-43261210 MS.gene81770:CDS
ATTGTGTGCCAAGTAAACTA+TGG 0.493367 1.3:+43260601 None:intergenic
TGGCTACGACAGAGAATGGT+AGG 0.504458 1.3:+43260891 None:intergenic
CAATTCTAGTTGCACCTACA+AGG 0.507515 1.3:-43261215 MS.gene81770:CDS
CACCTACAAGGCCTGGAGGA+TGG 0.513895 1.3:-43261203 MS.gene81770:CDS
ATGATGGCTACGACAGAGAA+TGG 0.518145 1.3:+43260887 None:intergenic
CTGATCACTTGTTCCCATTG+AGG 0.525536 1.3:+43261110 None:intergenic
AGCCTTGGTCCAGGCGCATG+GGG 0.531806 1.3:+43260860 None:intergenic
ATAAGGAAAAGCCTTGGTCC+AGG 0.552077 1.3:+43260851 None:intergenic
AAGATAATAAGGAAAAGCCT+TGG 0.569952 1.3:+43260845 None:intergenic
ATCACTTGTTCCCATTGAGG+CGG 0.575884 1.3:+43261113 None:intergenic
AGTTGCATCATTTATTACTG+TGG 0.594258 1.3:-43260504 MS.gene81770:CDS
GATTTCTGTGCACAGACAAG+TGG 0.595748 1.3:-43260533 MS.gene81770:CDS
ATAGTTTACTTGGCACACAA+TGG 0.619739 1.3:-43260599 MS.gene81770:CDS
AGAACCAAGCAACATTAACA+AGG 0.655647 1.3:-43261244 MS.gene81770:CDS
AAGGTTAGGTGCCATAGCAG+CGG 0.656083 1.3:-43261148 MS.gene81770:CDS
GGCTACGACAGAGAATGGTA+GGG 0.661247 1.3:+43260892 None:intergenic
GCCTTGGTCCAGGCGCATGG+GGG 0.665227 1.3:+43260861 None:intergenic
GTTGCACCTACAAGGCCTGG+AGG 0.707990 1.3:-43261207 MS.gene81770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTAATAAAAATATGATAA+CGG - chr1.3:43260950-43260969 MS.gene81770:intron 10.0%
!! ATTAAATTGTATACATCAAT+CGG + chr1.3:43260989-43261008 None:intergenic 15.0%
!! GTTAATAAAAATATGATAAC+GGG - chr1.3:43260951-43260970 MS.gene81770:intron 15.0%
!!! GTATTAGCTTATTATAATTA+TGG + chr1.3:43260745-43260764 None:intergenic 15.0%
!!! AACTGTGTTTATGAAATTTT+AGG - chr1.3:43261052-43261071 MS.gene81770:CDS 20.0%
!!! TTACGATATCTAAGATAATA+AGG + chr1.3:43260887-43260906 None:intergenic 20.0%
!!! CAGGTTTTTTGTTATTTCAA+TGG - chr1.3:43260776-43260795 MS.gene81770:intron 25.0%
AAGATAATAAGGAAAAGCCT+TGG + chr1.3:43260876-43260895 None:intergenic 30.0%
AGTTGCATCATTTATTACTG+TGG - chr1.3:43261214-43261233 MS.gene81770:CDS 30.0%
CCTTAGAATGAAATCCATTA+TGG + chr1.3:43260554-43260573 None:intergenic 30.0%
GAAAGAACTGAGTGAAAAAA+GGG + chr1.3:43260639-43260658 None:intergenic 30.0%
TGAAAGAACTGAGTGAAAAA+AGG + chr1.3:43260640-43260659 None:intergenic 30.0%
! TTTTCCTTGTTAATGTTGCT+TGG + chr1.3:43260481-43260500 None:intergenic 30.0%
!! ATGGATTTCATTCTAAGGTT+AGG - chr1.3:43260556-43260575 MS.gene81770:CDS 30.0%
!! ATTTTAGGTGTTTCTTACTC+TGG - chr1.3:43261067-43261086 MS.gene81770:CDS 30.0%
!! CCATAATGGATTTCATTCTA+AGG - chr1.3:43260551-43260570 MS.gene81770:CDS 30.0%
!! TAAAAATATGATAACGGGTG+CGG - chr1.3:43260956-43260975 MS.gene81770:intron 30.0%
!!! AAGTTTTGAGTGTTACATAC+TGG + chr1.3:43260696-43260715 None:intergenic 30.0%
!!! TGTGGTTGTTTTAATCTTGT+TGG - chr1.3:43261232-43261251 MS.gene81770:CDS 30.0%
AGAACCAAGCAACATTAACA+AGG - chr1.3:43260474-43260493 MS.gene81770:CDS 35.0%
ATAGTTTACTTGGCACACAA+TGG - chr1.3:43261119-43261138 MS.gene81770:CDS 35.0%
ATCTCCAAATTGGTTGCAAA+TGG + chr1.3:43261168-43261187 None:intergenic 35.0%
ATGGAAATCAAGACACAAAC+TGG - chr1.3:43261138-43261157 MS.gene81770:CDS 35.0%
ATTGTGTGCCAAGTAAACTA+TGG + chr1.3:43261120-43261139 None:intergenic 35.0%
GCTAATACTTGAAACAATGC+AGG - chr1.3:43260757-43260776 MS.gene81770:intron 35.0%
TTTGAGTGTTACATACTGGA+AGG + chr1.3:43260692-43260711 None:intergenic 35.0%
! TGTTAATGTTGCTTGGTTCT+GGG + chr1.3:43260474-43260493 None:intergenic 35.0%
! TTGTTAATGTTGCTTGGTTC+TGG + chr1.3:43260475-43260494 None:intergenic 35.0%
AGCTGCTTCCATAGTTTACT+TGG - chr1.3:43261109-43261128 MS.gene81770:CDS 40.0%
CTAGCCATTTGCAACCAATT+TGG - chr1.3:43261161-43261180 MS.gene81770:CDS 40.0%
GAAAAAGGGAACAGCCATAA+TGG - chr1.3:43260537-43260556 MS.gene81770:CDS 40.0%
GTGCACAGAAATCTCCAAAT+TGG + chr1.3:43261178-43261197 None:intergenic 40.0%
TGGTAGGGATAGAACTAAGT+AGG + chr1.3:43260814-43260833 None:intergenic 40.0%
! CAATTCTAGTTGCACCTACA+AGG - chr1.3:43260503-43260522 MS.gene81770:CDS 40.0%
ATAAGGAAAAGCCTTGGTCC+AGG + chr1.3:43260870-43260889 None:intergenic 45.0%
ATCACTTGTTCCCATTGAGG+CGG + chr1.3:43260608-43260627 None:intergenic 45.0%
ATGATGGCTACGACAGAGAA+TGG + chr1.3:43260834-43260853 None:intergenic 45.0%
CTGATCACTTGTTCCCATTG+AGG + chr1.3:43260611-43260630 None:intergenic 45.0%
GATTTCTGTGCACAGACAAG+TGG - chr1.3:43261185-43261204 MS.gene81770:CDS 45.0%
GTTGGTTGTGATGTCTGCTT+TGG - chr1.3:43261250-43261269 MS.gene81770:CDS 45.0%
!! TGATGTCTGCTTTGGCTCTT+GGG - chr1.3:43261258-43261277 MS.gene81770:CDS 45.0%
AAGGCCTGGAGGATGGAAAA+AGG - chr1.3:43260522-43260541 MS.gene81770:CDS 50.0%
AGGCCTGGAGGATGGAAAAA+GGG - chr1.3:43260523-43260542 MS.gene81770:CDS 50.0%
GGCTACGACAGAGAATGGTA+GGG + chr1.3:43260829-43260848 None:intergenic 50.0%
TGGCTACGACAGAGAATGGT+AGG + chr1.3:43260830-43260849 None:intergenic 50.0%
! AAGGTTAGGTGCCATAGCAG+CGG - chr1.3:43260570-43260589 MS.gene81770:CDS 50.0%
! GTTCCCTTTTTCCATCCTCC+AGG + chr1.3:43260529-43260548 None:intergenic 50.0%
!! GCTTGGTTCTGGGACATCAA+TGG + chr1.3:43260464-43260483 None:intergenic 50.0%
!! GTGATGTCTGCTTTGGCTCT+TGG - chr1.3:43261257-43261276 MS.gene81770:CDS 50.0%
AGCTGCAGCAGCACTTGAAG+TGG + chr1.3:43261093-43261112 None:intergenic 55.0%
CTAGTTGCACCTACAAGGCC+TGG - chr1.3:43260508-43260527 MS.gene81770:CDS 55.0%
! TTCCATCCTCCAGGCCTTGT+AGG + chr1.3:43260520-43260539 None:intergenic 55.0%
AAAGCCTTGGTCCAGGCGCA+TGG + chr1.3:43260863-43260882 None:intergenic 60.0%
AAGCCTTGGTCCAGGCGCAT+GGG + chr1.3:43260862-43260881 None:intergenic 60.0%
AGCGGCAATAGCCGCTGCTA+TGG + chr1.3:43260584-43260603 None:intergenic 60.0%
ATTGCCGCTGCCGCCTCAAT+GGG - chr1.3:43260595-43260614 MS.gene81770:CDS 60.0%
CACCTACAAGGCCTGGAGGA+TGG - chr1.3:43260515-43260534 MS.gene81770:CDS 60.0%
GTTGCACCTACAAGGCCTGG+AGG - chr1.3:43260511-43260530 MS.gene81770:CDS 60.0%
TATTGCCGCTGCCGCCTCAA+TGG - chr1.3:43260594-43260613 MS.gene81770:CDS 60.0%
TGTTCCCATTGAGGCGGCAG+CGG + chr1.3:43260602-43260621 None:intergenic 60.0%
AGCCTTGGTCCAGGCGCATG+GGG + chr1.3:43260861-43260880 None:intergenic 65.0%
AGGCGCATGGGGGCGAATGA+TGG + chr1.3:43260850-43260869 None:intergenic 65.0%
ATCATTCGCCCCCATGCGCC+TGG - chr1.3:43260849-43260868 MS.gene81770:CDS 65.0%
GCCTTGGTCCAGGCGCATGG+GGG + chr1.3:43260860-43260879 None:intergenic 70.0%
GCCCCCATGCGCCTGGACCA+AGG - chr1.3:43260856-43260875 MS.gene81770:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 43260451 43261289 43260451 ID=MS.gene81770
chr1.3 mRNA 43260451 43261289 43260451 ID=MS.gene81770.t1;Parent=MS.gene81770
chr1.3 exon 43261045 43261289 43261045 ID=MS.gene81770.t1.exon1;Parent=MS.gene81770.t1
chr1.3 CDS 43261045 43261289 43261045 ID=cds.MS.gene81770.t1;Parent=MS.gene81770.t1
chr1.3 exon 43260838 43260961 43260838 ID=MS.gene81770.t1.exon2;Parent=MS.gene81770.t1
chr1.3 CDS 43260838 43260961 43260838 ID=cds.MS.gene81770.t1;Parent=MS.gene81770.t1
chr1.3 exon 43260451 43260666 43260451 ID=MS.gene81770.t1.exon3;Parent=MS.gene81770.t1
chr1.3 CDS 43260451 43260666 43260451 ID=cds.MS.gene81770.t1;Parent=MS.gene81770.t1
Gene Sequence

>MS.gene81770

ATGTCAACAACCATTGATGTCCCAGAACCAAGCAACATTAACAAGGAAAAAACAATTCTAGTTGCACCTACAAGGCCTGGAGGATGGAAAAAGGGAACAGCCATAATGGATTTCATTCTAAGGTTAGGTGCCATAGCAGCGGCTATTGCCGCTGCCGCCTCAATGGGAACAAGTGATCAGACTCTCCCTTTTTTCACTCAGTTCTTTCAATTTGAAGCTAGCTATGATAGCTTTACAACCTTCCAGTATGTAACACTCAAAACTTTTTTTTTTTTCTTTCAAAACAATAATCCATAATTATAATAAGCTAATACTTGAAACAATGCAGGTTTTTTGTTATTTCAATGGCAATAGTAGCTTCCTACTTAGTTCTATCCCTACCATTCTCTGTCGTAGCCATCATTCGCCCCCATGCGCCTGGACCAAGGCTTTTCCTTATTATCTTAGATATCGTAAGCTTTTCTGTCTGTCTGTCTATCTATCTATCTATTCTATTTGTTGTTAATAAAAATATGATAACGGGTGCGGCAAGTGACCGATTGATGTATACAATTTAATTAAGTTCAATGTTAAATATTATCATAAATTAATTCAACTTTGAAACTGTGTTTATGAAATTTTAGGTGTTTCTTACTCTGGCCACTTCAAGTGCTGCTGCAGCTGCTTCCATAGTTTACTTGGCACACAATGGAAATCAAGACACAAACTGGCTAGCCATTTGCAACCAATTTGGAGATTTCTGTGCACAGACAAGTGGAGCAGTAGTTGCATCATTTATTACTGTGGTTGTTTTAATCTTGTTGGTTGTGATGTCTGCTTTGGCTCTTGGGAAGCATTGA

Protein sequence

>MS.gene81770.t1

MSTTIDVPEPSNINKEKTILVAPTRPGGWKKGTAIMDFILRLGAIAAAIAAAASMGTSDQTLPFFTQFFQFEASYDSFTTFQFFVISMAIVASYLVLSLPFSVVAIIRPHAPGPRLFLIILDIVFLTLATSSAAAAASIVYLAHNGNQDTNWLAICNQFGDFCAQTSGAVVASFITVVVLILLVVMSALALGKH