AlfalfaGEDB | MS.gene065729

M. sativa cultivar XinJiangDaYe / MS.gene065729

KEGG: -
GO: -
PFAM: PF04535.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065729.t1	XP_013467946.1	97.4	194	5	0	1	194	1	194	8.40E-93	349.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065729.t1	P0DI40	84.5	194	28	1	1	194	1	192	6.8e-83	308.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065729.t1	A0A072VKN9	97.4	194	5	0	1	194	1	194	6.1e-93	349.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene065729	0.818183	2.24E-52	-1.69E-46
MS.gene05104	MS.gene065729	0.800785	1.22E-48	-1.69E-46
MS.gene051996	MS.gene065729	0.852298	5.06E-61	-1.69E-46
MS.gene052211	MS.gene065729	0.804696	1.91E-49	-1.69E-46
MS.gene052223	MS.gene065729	0.831174	1.94E-55	-1.69E-46
MS.gene05240	MS.gene065729	0.84083	6.86E-58	-1.69E-46
MS.gene052706	MS.gene065729	0.809294	2.03E-50	-1.69E-46
MS.gene054138	MS.gene065729	0.809066	2.27E-50	-1.69E-46
MS.gene054277	MS.gene065729	0.864844	9.08E-65	-1.69E-46
MS.gene054742	MS.gene065729	0.857535	1.53E-62	-1.69E-46
MS.gene055215	MS.gene065729	0.806754	7.05E-50	-1.69E-46
MS.gene056076	MS.gene065729	0.808375	3.20E-50	-1.69E-46
MS.gene056330	MS.gene065729	0.834471	2.94E-56	-1.69E-46
MS.gene056590	MS.gene065729	0.802381	5.76E-49	-1.69E-46
MS.gene056920	MS.gene065729	0.883382	4.70E-71	-1.69E-46
MS.gene056922	MS.gene065729	0.867203	1.63E-65	-1.69E-46
MS.gene056923	MS.gene065729	0.954896	1.05E-112	-1.69E-46
MS.gene056924	MS.gene065729	0.941264	5.63E-101	-1.69E-46
MS.gene056925	MS.gene065729	0.912383	2.08E-83	-1.69E-46
MS.gene057261	MS.gene065729	0.836174	1.09E-56	-1.69E-46
MS.gene057544	MS.gene065729	0.807013	6.22E-50	-1.69E-46
MS.gene057820	MS.gene065729	0.802688	4.98E-49	-1.69E-46
MS.gene059039	MS.gene065729	0.815534	8.80E-52	-1.69E-46
MS.gene059328	MS.gene065729	0.808416	3.13E-50	-1.69E-46
MS.gene060087	MS.gene065729	0.845497	3.91E-59	-1.69E-46
MS.gene060205	MS.gene065729	0.800737	1.25E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene05032	MS.gene065729	PPI
MS.gene065729	MS.gene049677	PPI
MS.gene065729	MS.gene81804	PPI
MS.gene065729	MS.gene26200	PPI
MS.gene065729	MS.gene05033	PPI
MS.gene008354	MS.gene065729	PPI
MS.gene061583	MS.gene065729	PPI
MS.gene065729	MS.gene017305	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065729.t1	MTR_1g058570	97.423	194	5	0	1	194	1	194	2.51e-137	382
MS.gene065729.t1	MTR_1g058540	79.592	196	38	1	1	194	1	196	1.26e-101	291
MS.gene065729.t1	MTR_1g058560	87.179	195	24	1	1	194	1	195	2.32e-99	286
MS.gene065729.t1	MTR_2g086740	56.818	176	76	0	18	193	44	219	2.61e-57	180
MS.gene065729.t1	MTR_7g011090	49.180	183	89	1	12	194	8	186	5.65e-55	173
MS.gene065729.t1	MTR_4g081880	43.784	185	99	2	9	193	7	186	8.32e-49	157
MS.gene065729.t1	MTR_5g041900	46.108	167	90	0	28	194	20	186	9.08e-43	142
MS.gene065729.t1	MTR_2g086740	57.944	107	45	0	18	124	44	150	1.88e-32	114
MS.gene065729.t1	MTR_0004s0210	35.849	159	89	4	31	187	3	150	2.30e-18	78.6
MS.gene065729.t1	MTR_7g108130	28.049	164	113	3	31	191	24	185	9.21e-14	67.0
MS.gene065729.t1	MTR_0097s0070	31.356	118	79	2	71	187	69	185	9.44e-13	64.3
MS.gene065729.t1	MTR_8g089300	31.013	158	96	4	40	187	30	184	1.06e-11	61.2
MS.gene065729.t1	MTR_3g064310	32.203	118	77	2	74	191	77	191	4.60e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065729.t1	AT2G36100	58.416	202	75	1	2	194	5	206	1.60e-71	216
MS.gene065729.t1	AT5G06200	61.929	197	70	2	2	193	5	201	2.23e-64	197
MS.gene065729.t1	AT3G11550	61.000	200	71	2	2	194	5	204	6.13e-63	194
MS.gene065729.t1	AT2G27370	58.929	168	69	0	26	193	52	219	6.93e-61	189
MS.gene065729.t1	AT5G15290	48.000	150	78	0	25	174	17	166	1.27e-47	154
MS.gene065729.t1	AT1G14160	40.606	165	98	0	30	194	45	209	1.35e-41	139
MS.gene065729.t1	AT1G03700	32.215	149	92	2	39	187	12	151	7.16e-19	79.7
MS.gene065729.t1	AT4G03540	33.058	121	72	2	55	175	28	139	2.56e-15	70.5
MS.gene065729.t1	AT1G17200	25.397	189	129	4	7	191	1	181	2.74e-14	68.6
MS.gene065729.t1	AT4G15630	28.492	179	108	5	25	191	19	189	7.25e-14	67.4
MS.gene065729.t1	AT5G44550	30.263	152	97	3	40	183	22	172	2.64e-12	63.2
MS.gene065729.t1	AT3G06390	28.571	133	90	2	36	163	37	169	4.62e-12	62.4

Find 44 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTAATGTTGCTTGATTC+TGG	0.072454	1.2:+45992073	None:intergenic
CTAGCCATTTGCAACCAATT+TGG	0.242258	1.2:-45991382	MS.gene065729:CDS
GTGATGTCTGCTTTGGCTCT+TGG	0.283856	1.2:-45991286	MS.gene065729:CDS
AAGGCCTGGAGGATGGAAAA+AGG	0.302153	1.2:-45992023	MS.gene065729:CDS
CCTTAGAATGAAATCCATTA+TGG	0.309686	1.2:+45991994	None:intergenic
CCATAATGGATTTCATTCTA+AGG	0.312115	1.2:-45991994	MS.gene065729:CDS
TTCCATCCTCCAGGCCTTGT+AGG	0.319831	1.2:+45992028	None:intergenic
TTACGATATCTAAGATAATA+AGG	0.319981	1.2:+45991663	None:intergenic
TGATGTCTGCTTTGGCTCTT+GGG	0.345325	1.2:-45991285	MS.gene065729:CDS
TATTGCCGCTGCCGCCTCAA+TGG	0.357738	1.2:-45991951	MS.gene065729:CDS
ATTGCCGCTGCCGCCTCAAT+GGG	0.364904	1.2:-45991950	MS.gene065729:CDS
TGTTAATGTTGCTTGATTCT+GGG	0.378773	1.2:+45992074	None:intergenic
ATGGATTTCATTCTAAGGTT+AGG	0.379588	1.2:-45991989	MS.gene065729:CDS
AGCTGCTTCCATAGTTTACT+TGG	0.387801	1.2:-45991434	MS.gene065729:CDS
GTTGGTTGTGATGTCTGCTT+TGG	0.390211	1.2:-45991293	MS.gene065729:CDS
GTGCACAGAAATCTCCAAAT+TGG	0.409853	1.2:+45991368	None:intergenic
ATGGAAATCAAGACACAAAC+TGG	0.414877	1.2:-45991405	MS.gene065729:CDS
AGCTGCAGCAGCACTTGAAG+TGG	0.421850	1.2:+45991453	None:intergenic
AAGCCTTGGTCCAGGCGCAT+GGG	0.441787	1.2:+45991688	None:intergenic
AAAGCCTTGGTCCAGGCGCA+TGG	0.443522	1.2:+45991687	None:intergenic
AGCGGCAATAGCCGCTGCTA+TGG	0.444367	1.2:+45991964	None:intergenic
ATCTCCAAATTGGTTGCAAA+TGG	0.445100	1.2:+45991378	None:intergenic
TGGTAGGGATAGAACTAAGT+AGG	0.473587	1.2:+45991736	None:intergenic
TGTTCCCATTGAGGCGGCAG+CGG	0.482406	1.2:+45991946	None:intergenic
CTAGTTGCACCTACAAGGCC+TGG	0.489392	1.2:-45992037	MS.gene065729:CDS
ATTGTGTGCCAAGTAAACTA+TGG	0.493367	1.2:+45991426	None:intergenic
GCTTGATTCTGGGACATCAA+TGG	0.503870	1.2:+45992084	None:intergenic
TGGCTACGACAGAGAATGGT+AGG	0.504458	1.2:+45991720	None:intergenic
CAATTCTAGTTGCACCTACA+AGG	0.507515	1.2:-45992042	MS.gene065729:CDS
CACCTACAAGGCCTGGAGGA+TGG	0.513895	1.2:-45992030	MS.gene065729:CDS
ATGATGGCTACGACAGAGAA+TGG	0.518145	1.2:+45991716	None:intergenic
CTGATCACTTGTTCCCATTG+AGG	0.525536	1.2:+45991937	None:intergenic
AGCCTTGGTCCAGGCGCATG+GGG	0.531806	1.2:+45991689	None:intergenic
ATAAGGAAAAGCCTTGGTCC+AGG	0.552077	1.2:+45991680	None:intergenic
AAGATAATAAGGAAAAGCCT+TGG	0.569952	1.2:+45991674	None:intergenic
ATCACTTGTTCCCATTGAGG+CGG	0.575884	1.2:+45991940	None:intergenic
AGTTGCATCATTTATTACTG+TGG	0.594258	1.2:-45991329	MS.gene065729:CDS
ATAGTTTACTTGGCACACAA+TGG	0.619739	1.2:-45991424	MS.gene065729:CDS
AGAATCAAGCAACATTAACA+AGG	0.641758	1.2:-45992071	MS.gene065729:CDS
AAGGTTAGGTGCCATAGCAG+CGG	0.656083	1.2:-45991975	MS.gene065729:CDS
GGCTACGACAGAGAATGGTA+GGG	0.661247	1.2:+45991721	None:intergenic
GCCTTGGTCCAGGCGCATGG+GGG	0.665227	1.2:+45991690	None:intergenic
GATTTCTGTGCACAGACAAG+TGG	0.683686	1.2:-45991358	MS.gene065729:CDS
GTTGCACCTACAAGGCCTGG+AGG	0.707990	1.2:-45992034	MS.gene065729:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTTAATAAAAATATGATAA+GGG	-	chr1.2:45991777-45991796	MS.gene065729:intron	10.0%
!!	TTGTTAATAAAAATATGATA+AGG	-	chr1.2:45991776-45991795	MS.gene065729:intron	10.0%
!!	ATTAAATTGTATACATCAAT+CGG	+	chr1.2:45991816-45991835	None:intergenic	15.0%
!!	GTTAATAAAAATATGATAAG+GGG	-	chr1.2:45991778-45991797	MS.gene065729:intron	15.0%
!!!	GTATTAGCTTATTATAATTA+TGG	+	chr1.2:45991568-45991587	None:intergenic	15.0%
!!!	AACTGTGTTTATGAAATTTT+AGG	-	chr1.2:45991879-45991898	MS.gene065729:CDS	20.0%
!!!	TTACGATATCTAAGATAATA+AGG	+	chr1.2:45991710-45991729	None:intergenic	20.0%
!!!	CAGGTTTTTTGTTATTTCAA+TGG	-	chr1.2:45991599-45991618	MS.gene065729:intron	25.0%
	AAGATAATAAGGAAAAGCCT+TGG	+	chr1.2:45991699-45991718	None:intergenic	30.0%
	AGAATCAAGCAACATTAACA+AGG	-	chr1.2:45991299-45991318	MS.gene065729:CDS	30.0%
	AGTTGCATCATTTATTACTG+TGG	-	chr1.2:45992041-45992060	MS.gene065729:CDS	30.0%
	CCTTAGAATGAAATCCATTA+TGG	+	chr1.2:45991379-45991398	None:intergenic	30.0%
	GAAAGAACTGAGTGAAAAAA+GGG	+	chr1.2:45991464-45991483	None:intergenic	30.0%
	TAAAAATATGATAAGGGGTG+CGG	-	chr1.2:45991783-45991802	MS.gene065729:intron	30.0%
	TGAAAGAACTGAGTGAAAAA+AGG	+	chr1.2:45991465-45991484	None:intergenic	30.0%
!	TGTTAATGTTGCTTGATTCT+GGG	+	chr1.2:45991299-45991318	None:intergenic	30.0%
!	TTGTTAATGTTGCTTGATTC+TGG	+	chr1.2:45991300-45991319	None:intergenic	30.0%
!!	ATGGATTTCATTCTAAGGTT+AGG	-	chr1.2:45991381-45991400	MS.gene065729:CDS	30.0%
!!	ATTTTAGGTGTTTCTTACTC+TGG	-	chr1.2:45991894-45991913	MS.gene065729:CDS	30.0%
!!	CCATAATGGATTTCATTCTA+AGG	-	chr1.2:45991376-45991395	MS.gene065729:CDS	30.0%
!!!	AAGTTTTGAGTGTTACATAC+TGG	+	chr1.2:45991521-45991540	None:intergenic	30.0%
!!!	TGTGGTTGTTTTAATCTTGT+TGG	-	chr1.2:45992059-45992078	MS.gene065729:CDS	30.0%
	ATAGTTTACTTGGCACACAA+TGG	-	chr1.2:45991946-45991965	MS.gene065729:CDS	35.0%
	ATCTCCAAATTGGTTGCAAA+TGG	+	chr1.2:45991995-45992014	None:intergenic	35.0%
	ATGGAAATCAAGACACAAAC+TGG	-	chr1.2:45991965-45991984	MS.gene065729:CDS	35.0%
	ATTGTGTGCCAAGTAAACTA+TGG	+	chr1.2:45991947-45991966	None:intergenic	35.0%
	GCTAATACTTGAAACAATGC+AGG	-	chr1.2:45991580-45991599	MS.gene065729:intron	35.0%
	TTTGAGTGTTACATACTGGA+AGG	+	chr1.2:45991517-45991536	None:intergenic	35.0%
	AGCTGCTTCCATAGTTTACT+TGG	-	chr1.2:45991936-45991955	MS.gene065729:CDS	40.0%
	CTAGCCATTTGCAACCAATT+TGG	-	chr1.2:45991988-45992007	MS.gene065729:CDS	40.0%
	GAAAAAGGGAACAGCCATAA+TGG	-	chr1.2:45991362-45991381	MS.gene065729:CDS	40.0%
	GTGCACAGAAATCTCCAAAT+TGG	+	chr1.2:45992005-45992024	None:intergenic	40.0%
	TGGTAGGGATAGAACTAAGT+AGG	+	chr1.2:45991637-45991656	None:intergenic	40.0%
!	CAATTCTAGTTGCACCTACA+AGG	-	chr1.2:45991328-45991347	MS.gene065729:CDS	40.0%
	ATAAGGAAAAGCCTTGGTCC+AGG	+	chr1.2:45991693-45991712	None:intergenic	45.0%
	ATCACTTGTTCCCATTGAGG+CGG	+	chr1.2:45991433-45991452	None:intergenic	45.0%
	ATGATGGCTACGACAGAGAA+TGG	+	chr1.2:45991657-45991676	None:intergenic	45.0%
	CTGATCACTTGTTCCCATTG+AGG	+	chr1.2:45991436-45991455	None:intergenic	45.0%
	GATTTCTGTGCACAGACAAG+TGG	-	chr1.2:45992012-45992031	MS.gene065729:CDS	45.0%
	GTTGGTTGTGATGTCTGCTT+TGG	-	chr1.2:45992077-45992096	MS.gene065729:CDS	45.0%
!!	GCTTGATTCTGGGACATCAA+TGG	+	chr1.2:45991289-45991308	None:intergenic	45.0%
!!	TGATGTCTGCTTTGGCTCTT+GGG	-	chr1.2:45992085-45992104	MS.gene065729:CDS	45.0%
	AAGGCCTGGAGGATGGAAAA+AGG	-	chr1.2:45991347-45991366	MS.gene065729:CDS	50.0%
	AGGCCTGGAGGATGGAAAAA+GGG	-	chr1.2:45991348-45991367	MS.gene065729:CDS	50.0%
	GGCTACGACAGAGAATGGTA+GGG	+	chr1.2:45991652-45991671	None:intergenic	50.0%
	TGGCTACGACAGAGAATGGT+AGG	+	chr1.2:45991653-45991672	None:intergenic	50.0%
!	AAGGTTAGGTGCCATAGCAG+CGG	-	chr1.2:45991395-45991414	MS.gene065729:CDS	50.0%
!	GTTCCCTTTTTCCATCCTCC+AGG	+	chr1.2:45991354-45991373	None:intergenic	50.0%
!!	GTGATGTCTGCTTTGGCTCT+TGG	-	chr1.2:45992084-45992103	MS.gene065729:CDS	50.0%
	AGCTGCAGCAGCACTTGAAG+TGG	+	chr1.2:45991920-45991939	None:intergenic	55.0%
	CTAGTTGCACCTACAAGGCC+TGG	-	chr1.2:45991333-45991352	MS.gene065729:CDS	55.0%
!	TTCCATCCTCCAGGCCTTGT+AGG	+	chr1.2:45991345-45991364	None:intergenic	55.0%
	AAAGCCTTGGTCCAGGCGCA+TGG	+	chr1.2:45991686-45991705	None:intergenic	60.0%
	AAGCCTTGGTCCAGGCGCAT+GGG	+	chr1.2:45991685-45991704	None:intergenic	60.0%
	AGCGGCAATAGCCGCTGCTA+TGG	+	chr1.2:45991409-45991428	None:intergenic	60.0%
	ATTGCCGCTGCCGCCTCAAT+GGG	-	chr1.2:45991420-45991439	MS.gene065729:CDS	60.0%
	CACCTACAAGGCCTGGAGGA+TGG	-	chr1.2:45991340-45991359	MS.gene065729:CDS	60.0%
	GTTGCACCTACAAGGCCTGG+AGG	-	chr1.2:45991336-45991355	MS.gene065729:CDS	60.0%
	TATTGCCGCTGCCGCCTCAA+TGG	-	chr1.2:45991419-45991438	MS.gene065729:CDS	60.0%
	TGTTCCCATTGAGGCGGCAG+CGG	+	chr1.2:45991427-45991446	None:intergenic	60.0%
	AGCCTTGGTCCAGGCGCATG+GGG	+	chr1.2:45991684-45991703	None:intergenic	65.0%
	AGGCGCATGGGGGCGAATGA+TGG	+	chr1.2:45991673-45991692	None:intergenic	65.0%
	ATCATTCGCCCCCATGCGCC+TGG	-	chr1.2:45991672-45991691	MS.gene065729:CDS	65.0%
	GCCTTGGTCCAGGCGCATGG+GGG	+	chr1.2:45991683-45991702	None:intergenic	70.0%
	GCCCCCATGCGCCTGGACCA+AGG	-	chr1.2:45991679-45991698	MS.gene065729:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	45991276	45992116	45991276	ID=MS.gene065729
chr1.2	mRNA	45991276	45992116	45991276	ID=MS.gene065729.t1;Parent=MS.gene065729
chr1.2	exon	45991872	45992116	45991872	ID=MS.gene065729.t1.exon1;Parent=MS.gene065729.t1
chr1.2	CDS	45991872	45992116	45991872	ID=cds.MS.gene065729.t1;Parent=MS.gene065729.t1
chr1.2	exon	45991667	45991790	45991667	ID=MS.gene065729.t1.exon2;Parent=MS.gene065729.t1
chr1.2	CDS	45991667	45991790	45991667	ID=cds.MS.gene065729.t1;Parent=MS.gene065729.t1
chr1.2	exon	45991276	45991491	45991276	ID=MS.gene065729.t1.exon3;Parent=MS.gene065729.t1
chr1.2	CDS	45991276	45991491	45991276	ID=cds.MS.gene065729.t1;Parent=MS.gene065729.t1

Gene Sequence

>MS.gene065729

ATGTCAACAACCATTGATGTCCCAGAATCAAGCAACATTAACAAGGAAAAAACAATTCTAGTTGCACCTACAAGGCCTGGAGGATGGAAAAAGGGAACAGCCATAATGGATTTCATTCTAAGGTTAGGTGCCATAGCAGCGGCTATTGCCGCTGCCGCCTCAATGGGAACAAGTGATCAGACTCTCCCTTTTTTCACTCAGTTCTTTCAATTTGAAGCTAGCTATGATAGCTTTACAACCTTCCAGTATGTAACACTCAAAACTTTTTTTTTTCTTTCAAAACAATAATCCATAATTATAATAAGCTAATACTTGAAACAATGCAGGTTTTTTGTTATTTCAATGGCAATAGTAGCTTCCTACTTAGTTCTATCCCTACCATTCTCTGTCGTAGCCATCATTCGCCCCCATGCGCCTGGACCAAGGCTTTTCCTTATTATCTTAGATATCGTAAGCTTTTCTGTCTGTCTGTCTGTCTATCTATCTATCTATTCTATTTGTTGTTAATAAAAATATGATAAGGGGTGCGGCAAGTGACCGATTGATGTATACAATTTAATTAAGTTCAATGTTAAATATTATCATAAATTAATTCAACTTTGAAACTGTGTTTATGAAATTTTAGGTGTTTCTTACTCTGGCCACTTCAAGTGCTGCTGCAGCTGCTTCCATAGTTTACTTGGCACACAATGGAAATCAAGACACAAACTGGCTAGCCATTTGCAACCAATTTGGAGATTTCTGTGCACAGACAAGTGGTGCAGTAGTTGCATCATTTATTACTGTGGTTGTTTTAATCTTGTTGGTTGTGATGTCTGCTTTGGCTCTTGGGAAGCATTGA

Protein sequence

>MS.gene065729.t1

MSTTIDVPESSNINKEKTILVAPTRPGGWKKGTAIMDFILRLGAIAAAIAAAASMGTSDQTLPFFTQFFQFEASYDSFTTFQFFVISMAIVASYLVLSLPFSVVAIIRPHAPGPRLFLIILDIVFLTLATSSAAAAASIVYLAHNGNQDTNWLAICNQFGDFCAQTSGAVVASFITVVVLILLVVMSALALGKH