Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33644.t1 | XP_013466443.1 | 90.1 | 223 | 21 | 1 | 1 | 223 | 1 | 222 | 8.10E-101 | 376.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33644.t1 | A0A072VEP6 | 90.1 | 223 | 21 | 1 | 1 | 223 | 1 | 222 | 5.8e-101 | 376.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene33644 | 0.838559 | 2.68E-57 | -1.69E-46 |
| MS.gene049159 | MS.gene33644 | 0.822089 | 2.85E-53 | -1.69E-46 |
| MS.gene04918 | MS.gene33644 | 0.82994 | 3.89E-55 | -1.69E-46 |
| MS.gene04919 | MS.gene33644 | 0.803583 | 3.25E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene33644 | 0.832965 | 6.99E-56 | -1.69E-46 |
| MS.gene049254 | MS.gene33644 | 0.848014 | 8.03E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene33644 | 0.846979 | 1.55E-59 | -1.69E-46 |
| MS.gene049343 | MS.gene33644 | 0.800935 | 1.14E-48 | -1.69E-46 |
| MS.gene049368 | MS.gene33644 | 0.825055 | 5.77E-54 | -1.69E-46 |
| MS.gene049404 | MS.gene33644 | 0.805907 | 1.06E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene33644 | -0.806728 | 7.15E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene33644 | 0.818019 | 2.44E-52 | -1.69E-46 |
| MS.gene049452 | MS.gene33644 | 0.845219 | 4.65E-59 | -1.69E-46 |
| MS.gene049499 | MS.gene33644 | 0.862505 | 4.83E-64 | -1.69E-46 |
| MS.gene049711 | MS.gene33644 | 0.814123 | 1.81E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene33644 | 0.856617 | 2.85E-62 | -1.69E-46 |
| MS.gene050026 | MS.gene33644 | 0.813186 | 2.91E-51 | -1.69E-46 |
| MS.gene050273 | MS.gene33644 | 0.801828 | 7.49E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene33644 | 0.801114 | 1.05E-48 | -1.69E-46 |
| MS.gene050559 | MS.gene33644 | 0.832382 | 9.76E-56 | -1.69E-46 |
| MS.gene050561 | MS.gene33644 | 0.835345 | 1.77E-56 | -1.69E-46 |
| MS.gene050562 | MS.gene33644 | 0.825542 | 4.43E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene33644 | 0.815293 | 9.96E-52 | -1.69E-46 |
| MS.gene050680 | MS.gene33644 | 0.801036 | 1.09E-48 | -1.69E-46 |
| MS.gene050759 | MS.gene33644 | 0.828993 | 6.60E-55 | -1.69E-46 |
| MS.gene050953 | MS.gene33644 | 0.80438 | 2.22E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene33644 | 0.814912 | 1.21E-51 | -1.69E-46 |
| MS.gene051066 | MS.gene33644 | 0.810509 | 1.11E-50 | -1.69E-46 |
| MS.gene051181 | MS.gene33644 | 0.803142 | 4.02E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene33644 | 0.841531 | 4.49E-58 | -1.69E-46 |
| MS.gene051279 | MS.gene33644 | 0.834598 | 2.73E-56 | -1.69E-46 |
| MS.gene051332 | MS.gene33644 | 0.81882 | 1.61E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene33644 | 0.801324 | 9.50E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene33644 | 0.810572 | 1.08E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene33644 | 0.800863 | 1.18E-48 | -1.69E-46 |
| MS.gene051597 | MS.gene33644 | 0.823455 | 1.37E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene33644 | 0.829298 | 5.57E-55 | -1.69E-46 |
| MS.gene051800 | MS.gene33644 | 0.826299 | 2.93E-54 | -1.69E-46 |
| MS.gene051856 | MS.gene33644 | 0.812185 | 4.82E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene33644 | 0.812208 | 4.76E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene33644 | 0.806319 | 8.72E-50 | -1.69E-46 |
| MS.gene052037 | MS.gene33644 | 0.8146 | 1.42E-51 | -1.69E-46 |
| MS.gene052239 | MS.gene33644 | -0.817599 | 3.03E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene33644 | 0.81682 | 4.54E-52 | -1.69E-46 |
| MS.gene052518 | MS.gene33644 | 0.803943 | 2.74E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene33644 | 0.845087 | 5.05E-59 | -1.69E-46 |
| MS.gene052534 | MS.gene33644 | 0.819746 | 9.88E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene33644 | 0.825108 | 5.61E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene33644 | 0.834954 | 2.22E-56 | -1.69E-46 |
| MS.gene052586 | MS.gene33644 | 0.813476 | 2.51E-51 | -1.69E-46 |
| MS.gene052674 | MS.gene33644 | 0.817263 | 3.61E-52 | -1.69E-46 |
| MS.gene052675 | MS.gene33644 | 0.820929 | 5.29E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene33644 | 0.826061 | 3.34E-54 | -1.69E-46 |
| MS.gene052773 | MS.gene33644 | 0.826395 | 2.78E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene33644 | 0.802647 | 5.08E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene33644 | 0.822559 | 2.22E-53 | -1.69E-46 |
| MS.gene053079 | MS.gene33644 | 0.825615 | 4.26E-54 | -1.69E-46 |
| MS.gene05308 | MS.gene33644 | 0.830456 | 2.91E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene33644 | 0.859773 | 3.28E-63 | -1.69E-46 |
| MS.gene053272 | MS.gene33644 | -0.8263 | 2.93E-54 | -1.69E-46 |
| MS.gene053321 | MS.gene33644 | 0.801858 | 7.38E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene33644 | 0.820918 | 5.32E-53 | -1.69E-46 |
| MS.gene053931 | MS.gene33644 | 0.870685 | 1.21E-66 | -1.69E-46 |
| MS.gene053963 | MS.gene33644 | 0.805249 | 1.46E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene33644 | 0.803787 | 2.95E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene33644 | 0.803529 | 3.34E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene33644 | 0.829945 | 3.87E-55 | -1.69E-46 |
| MS.gene054236 | MS.gene33644 | 0.810483 | 1.13E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene33644 | 0.840586 | 7.96E-58 | -1.69E-46 |
| MS.gene054291 | MS.gene33644 | -0.805344 | 1.40E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene33644 | 0.823844 | 1.11E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene33644 | 0.822199 | 2.69E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene33644 | 0.849898 | 2.41E-60 | -1.69E-46 |
| MS.gene054641 | MS.gene33644 | 0.801734 | 7.83E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene33644 | 0.843137 | 1.69E-58 | -1.69E-46 |
| MS.gene054657 | MS.gene33644 | 0.833408 | 5.42E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene33644 | 0.833842 | 4.23E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene33644 | 0.816312 | 5.90E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene33644 | 0.823029 | 1.73E-53 | -1.69E-46 |
| MS.gene05510 | MS.gene33644 | 0.809088 | 2.25E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene33644 | 0.823918 | 1.07E-53 | -1.69E-46 |
| MS.gene055222 | MS.gene33644 | 0.811181 | 7.96E-51 | -1.69E-46 |
| MS.gene055227 | MS.gene33644 | 0.811907 | 5.53E-51 | -1.69E-46 |
| MS.gene055228 | MS.gene33644 | 0.828293 | 9.74E-55 | -1.69E-46 |
| MS.gene055513 | MS.gene33644 | 0.80223 | 6.19E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene33644 | 0.827411 | 1.59E-54 | -1.69E-46 |
| MS.gene055589 | MS.gene33644 | 0.809633 | 1.72E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene33644 | 0.817056 | 4.02E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene33644 | 0.803861 | 2.85E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene33644 | 0.802954 | 4.39E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene33644 | 0.820671 | 6.06E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene33644 | 0.835824 | 1.34E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene33644 | 0.826673 | 2.38E-54 | -1.69E-46 |
| MS.gene056045 | MS.gene33644 | 0.817944 | 2.54E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene33644 | 0.814199 | 1.74E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene33644 | 0.851627 | 7.85E-61 | -1.69E-46 |
| MS.gene056174 | MS.gene33644 | 0.846787 | 1.74E-59 | -1.69E-46 |
| MS.gene056215 | MS.gene33644 | 0.802948 | 4.40E-49 | -1.69E-46 |
| MS.gene056252 | MS.gene33644 | 0.811417 | 7.07E-51 | -1.69E-46 |
| MS.gene056308 | MS.gene33644 | 0.805845 | 1.10E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene33644 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene33644 | 0.800214 | 1.60E-48 | -1.69E-46 |
| MS.gene056699 | MS.gene33644 | 0.804429 | 2.17E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene33644 | 0.861994 | 6.94E-64 | -1.69E-46 |
| MS.gene056779 | MS.gene33644 | 0.80475 | 1.86E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene33644 | 0.864312 | 1.33E-64 | -1.69E-46 |
| MS.gene05690 | MS.gene33644 | 0.824208 | 9.14E-54 | -1.69E-46 |
| MS.gene056973 | MS.gene33644 | 0.834377 | 3.11E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene33644 | 0.80295 | 4.40E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene33644 | 0.866553 | 2.62E-65 | -1.69E-46 |
| MS.gene057444 | MS.gene33644 | 0.805719 | 1.17E-49 | -1.69E-46 |
| MS.gene057598 | MS.gene33644 | 0.842526 | 2.45E-58 | -1.69E-46 |
| MS.gene057693 | MS.gene33644 | 0.822646 | 2.12E-53 | -1.69E-46 |
| MS.gene057855 | MS.gene33644 | 0.819762 | 9.79E-53 | -1.69E-46 |
| MS.gene057890 | MS.gene33644 | -0.816077 | 6.66E-52 | -1.69E-46 |
| MS.gene057893 | MS.gene33644 | -0.808785 | 2.61E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene33644 | 0.821123 | 4.77E-53 | -1.69E-46 |
| MS.gene058009 | MS.gene33644 | 0.841524 | 4.51E-58 | -1.69E-46 |
| MS.gene058010 | MS.gene33644 | 0.851907 | 6.54E-61 | -1.69E-46 |
| MS.gene058146 | MS.gene33644 | 0.803347 | 3.64E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene33644 | 0.819209 | 1.31E-52 | -1.69E-46 |
| MS.gene058223 | MS.gene33644 | -0.815604 | 8.49E-52 | -1.69E-46 |
| MS.gene058279 | MS.gene33644 | 0.816452 | 5.49E-52 | -1.69E-46 |
| MS.gene058292 | MS.gene33644 | 0.813223 | 2.85E-51 | -1.69E-46 |
| MS.gene05847 | MS.gene33644 | 0.837839 | 4.10E-57 | -1.69E-46 |
| MS.gene058548 | MS.gene33644 | 0.804266 | 2.35E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene33644 | 0.840342 | 9.21E-58 | -1.69E-46 |
| MS.gene058783 | MS.gene33644 | 0.850666 | 1.47E-60 | -1.69E-46 |
| MS.gene058836 | MS.gene33644 | 0.802938 | 4.42E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene33644 | 0.835786 | 1.37E-56 | -1.69E-46 |
| MS.gene05897 | MS.gene33644 | 0.831345 | 1.76E-55 | -1.69E-46 |
| MS.gene059146 | MS.gene33644 | 0.805236 | 1.47E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene33644 | 0.803852 | 2.86E-49 | -1.69E-46 |
| MS.gene059224 | MS.gene33644 | 0.819276 | 1.26E-52 | -1.69E-46 |
| MS.gene059283 | MS.gene33644 | 0.808356 | 3.22E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene33644 | 0.804112 | 2.53E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene33644 | 0.811232 | 7.76E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene33644 | 0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene33644 | 0.840969 | 6.31E-58 | -1.69E-46 |
| MS.gene059842 | MS.gene33644 | 0.837471 | 5.10E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene33644 | -0.827646 | 1.39E-54 | -1.69E-46 |
| MS.gene060011 | MS.gene33644 | 0.819374 | 1.20E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene33644 | 0.827157 | 1.83E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene33644 | 0.829436 | 5.15E-55 | -1.69E-46 |
| MS.gene060310 | MS.gene33644 | 0.822349 | 2.48E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene33644 | 0.817286 | 3.57E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene33644 | 0.812966 | 3.25E-51 | -1.69E-46 |
| MS.gene060435 | MS.gene33644 | 0.804126 | 2.51E-49 | -1.69E-46 |
| MS.gene060520 | MS.gene33644 | 0.806262 | 8.96E-50 | -1.69E-46 |
| MS.gene060527 | MS.gene33644 | 0.807744 | 4.35E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene33644 | 0.800537 | 1.38E-48 | -1.69E-46 |
| MS.gene060777 | MS.gene33644 | 0.830508 | 2.82E-55 | -1.69E-46 |
| MS.gene060905 | MS.gene33644 | 0.803024 | 4.25E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene33644 | 0.817864 | 2.64E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene33644 | 0.825848 | 3.75E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene33644 | 0.863953 | 1.72E-64 | -1.69E-46 |
| MS.gene060937 | MS.gene33644 | 0.857426 | 1.65E-62 | -1.69E-46 |
| MS.gene061100 | MS.gene33644 | 0.832123 | 1.13E-55 | -1.69E-46 |
| MS.gene061160 | MS.gene33644 | 0.825907 | 3.63E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33644.t1 | MTR_1g029550 | 91.928 | 223 | 17 | 1 | 1 | 223 | 1 | 222 | 1.40e-139 | 392 |
| MS.gene33644.t1 | MTR_3g088815 | 38.208 | 212 | 99 | 8 | 1 | 199 | 1 | 193 | 5.00e-24 | 97.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33644.t1 | AT1G76980 | 41.379 | 174 | 81 | 6 | 49 | 204 | 28 | 198 | 1.65e-27 | 106 |
| MS.gene33644.t1 | AT1G76980 | 41.379 | 174 | 81 | 6 | 49 | 204 | 28 | 198 | 2.73e-27 | 105 |
| MS.gene33644.t1 | AT1G21390 | 38.342 | 193 | 88 | 6 | 33 | 199 | 5 | 192 | 1.94e-26 | 103 |
Find 47 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATGTGTTCTCCACCTTTA+TGG | 0.164256 | 1.1:+15072686 | MS.gene33644:CDS |
| TTAGATGATCCCAAAGAAAT+TGG | 0.202956 | 1.1:-15073000 | None:intergenic |
| TGATAAGAAGAAAGAGTTAT+TGG | 0.226827 | 1.1:+15073029 | MS.gene33644:CDS |
| AAGAGGTCAAAGAGAGTTAA+TGG | 0.266398 | 1.1:+15072774 | MS.gene33644:CDS |
| ATGGCTTGTGTCTTTGATTC+AGG | 0.274722 | 1.1:-15072748 | None:intergenic |
| GTAAGTTCATAGCTTGATTC+TGG | 0.277190 | 1.1:-15072814 | None:intergenic |
| TTACTCTTTGTTGTCCATAA+AGG | 0.323877 | 1.1:-15072700 | None:intergenic |
| TGTATTCTATCATCAGAAAC+TGG | 0.389778 | 1.1:-15072586 | None:intergenic |
| CTCTCTTTGACCTCTTTCAA+TGG | 0.402619 | 1.1:-15072767 | None:intergenic |
| GGTAACACTATTGTTATTGT+TGG | 0.418413 | 1.1:-15072623 | None:intergenic |
| TTCTGATGGATCTGTTAGTA+AGG | 0.426899 | 1.1:+15073101 | MS.gene33644:CDS |
| GGATTGGTGGAAGAAGAAAC+TGG | 0.436996 | 1.1:+15073122 | MS.gene33644:CDS |
| TCAAAGAGAGTTAATGGAAA+TGG | 0.447636 | 1.1:+15072780 | MS.gene33644:CDS |
| AAGAAATTGTGGGTTGGTAA+TGG | 0.479830 | 1.1:-15072561 | None:intergenic |
| CTCTTTGTTGTCCATAAAGG+TGG | 0.488907 | 1.1:-15072697 | None:intergenic |
| CCAAGAGCATCTGTTTCTGA+TGG | 0.489074 | 1.1:+15073087 | MS.gene33644:CDS |
| AGAAACTGGAAAAGAAATTG+TGG | 0.502575 | 1.1:-15072572 | None:intergenic |
| GATCTGTTAGTAAGGATTGG+TGG | 0.520890 | 1.1:+15073109 | MS.gene33644:CDS |
| TAAAAGTAGTAGTAATTCAA+AGG | 0.523482 | 1.1:+15073059 | MS.gene33644:CDS |
| GAAACTGGAAAAGAAATTGT+GGG | 0.525639 | 1.1:-15072571 | None:intergenic |
| CAAAGTGAAACATGACTAGC+CGG | 0.535783 | 1.1:-15073526 | None:intergenic |
| AAGACACAAGCCATTGAAAG+AGG | 0.538043 | 1.1:+15072757 | MS.gene33644:CDS |
| CCATCAGAAACAGATGCTCT+TGG | 0.545084 | 1.1:-15073087 | None:intergenic |
| AGGTGAAAGACTTCTATAGT+TGG | 0.545704 | 1.1:-15072728 | None:intergenic |
| TATTCTAGCGAAGGAGACCC+CGG | 0.553212 | 1.1:+15073507 | MS.gene33644:CDS |
| GGGAGTAGTGGTTCAGAATG+TGG | 0.556003 | 1.1:+15073144 | MS.gene33644:CDS |
| AAAATAACAAGGTAAAAGAG+AGG | 0.570398 | 1.1:+15072872 | MS.gene33644:CDS |
| CTGGAAAAGAAATTGTGGGT+TGG | 0.579799 | 1.1:-15072567 | None:intergenic |
| AGAAGAGTATTGACAGTGGA+GGG | 0.591275 | 1.1:+15072956 | MS.gene33644:CDS |
| ATGGATCTGTTAGTAAGGAT+TGG | 0.591287 | 1.1:+15073106 | MS.gene33644:CDS |
| GGTTGGTAATGGTATCTTCG+TGG | 0.594866 | 1.1:-15072550 | None:intergenic |
| AAGAAGAAACTGGGGAGTAG+TGG | 0.594902 | 1.1:+15073132 | MS.gene33644:CDS |
| GATTGGTGGAAGAAGAAACT+GGG | 0.596215 | 1.1:+15073123 | MS.gene33644:CDS |
| AAAGTGAAACATGACTAGCC+GGG | 0.607958 | 1.1:-15073525 | None:intergenic |
| AATTAGGGCTAGTAGAAACA+TGG | 0.610092 | 1.1:+15072930 | MS.gene33644:CDS |
| ATTGACTCGTATTCTAGCGA+AGG | 0.610173 | 1.1:+15073498 | MS.gene33644:CDS |
| AAAGGTGGAGAACACATACC+AGG | 0.623685 | 1.1:-15072682 | None:intergenic |
| TACTTTGAAAGATCTTGTTG+AGG | 0.634719 | 1.1:+15072834 | MS.gene33644:CDS |
| AGTGGTGTATCTAGCATGAG+TGG | 0.636022 | 1.1:+15073186 | MS.gene33644:CDS |
| AAGTGAAACATGACTAGCCG+GGG | 0.647231 | 1.1:-15073524 | None:intergenic |
| AAGAAGAGTATTGACAGTGG+AGG | 0.647384 | 1.1:+15072955 | MS.gene33644:CDS |
| ATTGGTGGAAGAAGAAACTG+GGG | 0.670609 | 1.1:+15073124 | MS.gene33644:CDS |
| GGTCATGTTGAAGATGAACC+TGG | 0.675151 | 1.1:+15072664 | MS.gene33644:CDS |
| AGTAGTGGTTCAGAATGTGG+AGG | 0.678513 | 1.1:+15073147 | MS.gene33644:CDS |
| TCATGTTGAAGAAAATAACA+AGG | 0.690119 | 1.1:+15072861 | MS.gene33644:CDS |
| GATAAGAAGAGTATTGACAG+TGG | 0.692316 | 1.1:+15072952 | MS.gene33644:CDS |
| GGTGATCAAAGTGATCATAG+TGG | 0.721338 | 1.1:+15073168 | MS.gene33644:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATATATAAATGTTTGAA+TGG | + | chr1.1:15073330-15073349 | MS.gene33644:intron | 10.0% |
| !! | TTTGAAAGGAAAAAAAAAAA+AGG | - | chr1.1:15073444-15073463 | None:intergenic | 15.0% |
| !! | TAAAAGTAGTAGTAATTCAA+AGG | + | chr1.1:15073059-15073078 | MS.gene33644:CDS | 20.0% |
| !!! | AATGATTTTTCCAATTTCTT+TGG | + | chr1.1:15072990-15073009 | MS.gene33644:CDS | 20.0% |
| !!! | ATGATTTTTCCAATTTCTTT+GGG | + | chr1.1:15072991-15073010 | MS.gene33644:CDS | 20.0% |
| !!! | CTATTGTAAGTTTTAGATTT+TGG | + | chr1.1:15073387-15073406 | MS.gene33644:intron | 20.0% |
| !!! | TTTTTTTCCTTTCAAACTAT+GGG | + | chr1.1:15073448-15073467 | MS.gene33644:intron | 20.0% |
| !!! | TTTTTTTTCCTTTCAAACTA+TGG | + | chr1.1:15073447-15073466 | MS.gene33644:intron | 20.0% |
| ! | AAAATAACAAGGTAAAAGAG+AGG | + | chr1.1:15072872-15072891 | MS.gene33644:CDS | 25.0% |
| ! | TCATGTTGAAGAAAATAACA+AGG | + | chr1.1:15072861-15072880 | MS.gene33644:CDS | 25.0% |
| ! | TGATAAGAAGAAAGAGTTAT+TGG | + | chr1.1:15073029-15073048 | MS.gene33644:CDS | 25.0% |
| AAAAACACACGAAAAAGAGA+AGG | + | chr1.1:15072907-15072926 | MS.gene33644:CDS | 30.0% | |
| AACAAATCCCATAGTTTGAA+AGG | - | chr1.1:15073458-15073477 | None:intergenic | 30.0% | |
| ACCAAAAACTACAATCATCA+TGG | + | chr1.1:15072643-15072662 | MS.gene33644:CDS | 30.0% | |
| ACGAAAAAGAGAAGGAATTA+GGG | + | chr1.1:15072915-15072934 | MS.gene33644:CDS | 30.0% | |
| AGAAACTGGAAAAGAAATTG+TGG | - | chr1.1:15072575-15072594 | None:intergenic | 30.0% | |
| GAAACTGGAAAAGAAATTGT+GGG | - | chr1.1:15072574-15072593 | None:intergenic | 30.0% | |
| TCAAAGAGAGTTAATGGAAA+TGG | + | chr1.1:15072780-15072799 | MS.gene33644:CDS | 30.0% | |
| TGTATTCTATCATCAGAAAC+TGG | - | chr1.1:15072589-15072608 | None:intergenic | 30.0% | |
| TTAGATGATCCCAAAGAAAT+TGG | - | chr1.1:15073003-15073022 | None:intergenic | 30.0% | |
| ! | ACCATGATGATTGTAGTTTT+TGG | - | chr1.1:15072647-15072666 | None:intergenic | 30.0% |
| ! | GGTAACACTATTGTTATTGT+TGG | - | chr1.1:15072626-15072645 | None:intergenic | 30.0% |
| ! | TACTTTGAAAGATCTTGTTG+AGG | + | chr1.1:15072834-15072853 | MS.gene33644:CDS | 30.0% |
| ! | TTACTCTTTGTTGTCCATAA+AGG | - | chr1.1:15072703-15072722 | None:intergenic | 30.0% |
| !!! | GTAAGTTTTAGATTTTGGCA+TGG | + | chr1.1:15073392-15073411 | MS.gene33644:intron | 30.0% |
| AAAAAAGGATTGCTCCTTGT+AGG | - | chr1.1:15073429-15073448 | None:intergenic | 35.0% | |
| AAGAGGTCAAAGAGAGTTAA+TGG | + | chr1.1:15072774-15072793 | MS.gene33644:CDS | 35.0% | |
| AGGTGAAAGACTTCTATAGT+TGG | - | chr1.1:15072731-15072750 | None:intergenic | 35.0% | |
| AGTGGTAGCATCAAAAGATA+TGG | + | chr1.1:15073204-15073223 | MS.gene33644:intron | 35.0% | |
| ATGGATCTGTTAGTAAGGAT+TGG | + | chr1.1:15073106-15073125 | MS.gene33644:CDS | 35.0% | |
| CACGAAAAAGAGAAGGAATT+AGG | + | chr1.1:15072914-15072933 | MS.gene33644:CDS | 35.0% | |
| GTAAGTTCATAGCTTGATTC+TGG | - | chr1.1:15072817-15072836 | None:intergenic | 35.0% | |
| TGGTAATTAACAAGCCTACA+AGG | + | chr1.1:15073412-15073431 | MS.gene33644:intron | 35.0% | |
| ! | AATTAGGGCTAGTAGAAACA+TGG | + | chr1.1:15072930-15072949 | MS.gene33644:CDS | 35.0% |
| ! | ACTATGGGATTTGTTTCGTT+AGG | + | chr1.1:15073463-15073482 | MS.gene33644:intron | 35.0% |
| ! | TTCTGATGGATCTGTTAGTA+AGG | + | chr1.1:15073101-15073120 | MS.gene33644:CDS | 35.0% |
| !! | AAGAAATTGTGGGTTGGTAA+TGG | - | chr1.1:15072564-15072583 | None:intergenic | 35.0% |
| !! | GATAAGAAGAGTATTGACAG+TGG | + | chr1.1:15072952-15072971 | MS.gene33644:CDS | 35.0% |
| AAGACACAAGCCATTGAAAG+AGG | + | chr1.1:15072757-15072776 | MS.gene33644:CDS | 40.0% | |
| ATTGGTGGAAGAAGAAACTG+GGG | + | chr1.1:15073124-15073143 | MS.gene33644:CDS | 40.0% | |
| CAAAGTGAAACATGACTAGC+CGG | - | chr1.1:15073529-15073548 | None:intergenic | 40.0% | |
| CTCTCTTTGACCTCTTTCAA+TGG | - | chr1.1:15072770-15072789 | None:intergenic | 40.0% | |
| CTGGAAAAGAAATTGTGGGT+TGG | - | chr1.1:15072570-15072589 | None:intergenic | 40.0% | |
| GATCTGTTAGTAAGGATTGG+TGG | + | chr1.1:15073109-15073128 | MS.gene33644:CDS | 40.0% | |
| GATTGGTGGAAGAAGAAACT+GGG | + | chr1.1:15073123-15073142 | MS.gene33644:CDS | 40.0% | |
| GGTGATCAAAGTGATCATAG+TGG | + | chr1.1:15073168-15073187 | MS.gene33644:CDS | 40.0% | |
| GTATGTGTTCTCCACCTTTA+TGG | + | chr1.1:15072686-15072705 | MS.gene33644:CDS | 40.0% | |
| ! | AAAGTGAAACATGACTAGCC+GGG | - | chr1.1:15073528-15073547 | None:intergenic | 40.0% |
| ! | ATGGCTTGTGTCTTTGATTC+AGG | - | chr1.1:15072751-15072770 | None:intergenic | 40.0% |
| ! | CTCTTTGTTGTCCATAAAGG+TGG | - | chr1.1:15072700-15072719 | None:intergenic | 40.0% |
| !! | AAGAAGAGTATTGACAGTGG+AGG | + | chr1.1:15072955-15072974 | MS.gene33644:CDS | 40.0% |
| !! | AGAAGAGTATTGACAGTGGA+GGG | + | chr1.1:15072956-15072975 | MS.gene33644:CDS | 40.0% |
| !! | ATTGACTCGTATTCTAGCGA+AGG | + | chr1.1:15073498-15073517 | MS.gene33644:CDS | 40.0% |
| AAAGGTGGAGAACACATACC+AGG | - | chr1.1:15072685-15072704 | None:intergenic | 45.0% | |
| AAGAAGAAACTGGGGAGTAG+TGG | + | chr1.1:15073132-15073151 | MS.gene33644:CDS | 45.0% | |
| AGTAGTGGTTCAGAATGTGG+AGG | + | chr1.1:15073147-15073166 | MS.gene33644:CDS | 45.0% | |
| AGTGGTGTATCTAGCATGAG+TGG | + | chr1.1:15073186-15073205 | MS.gene33644:CDS | 45.0% | |
| CCATCAGAAACAGATGCTCT+TGG | - | chr1.1:15073090-15073109 | None:intergenic | 45.0% | |
| GGTCATGTTGAAGATGAACC+TGG | + | chr1.1:15072664-15072683 | MS.gene33644:CDS | 45.0% | |
| ! | AAGTGAAACATGACTAGCCG+GGG | - | chr1.1:15073527-15073546 | None:intergenic | 45.0% |
| ! | GGATTGGTGGAAGAAGAAAC+TGG | + | chr1.1:15073122-15073141 | MS.gene33644:CDS | 45.0% |
| !! | CCAAGAGCATCTGTTTCTGA+TGG | + | chr1.1:15073087-15073106 | MS.gene33644:CDS | 45.0% |
| !! | GGTTGGTAATGGTATCTTCG+TGG | - | chr1.1:15072553-15072572 | None:intergenic | 45.0% |
| !! | AAAAATTACTTATAATTATA+TGG | - | chr1.1:15073288-15073307 | None:intergenic | 5.0% |
| ACTCCTGCTGCTACAACTAC+TGG | - | chr1.1:15073241-15073260 | None:intergenic | 50.0% | |
| GAACCAGTAGTTGTAGCAGC+AGG | + | chr1.1:15073235-15073254 | MS.gene33644:intron | 50.0% | |
| GGGAGTAGTGGTTCAGAATG+TGG | + | chr1.1:15073144-15073163 | MS.gene33644:CDS | 50.0% | |
| GTAGCAGCAGGAGTAATAGC+AGG | + | chr1.1:15073247-15073266 | MS.gene33644:intron | 50.0% | |
| TATTCTAGCGAAGGAGACCC+CGG | + | chr1.1:15073507-15073526 | MS.gene33644:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 15072541 | 15073549 | 15072541 | ID=MS.gene33644 |
| chr1.1 | mRNA | 15072541 | 15073549 | 15072541 | ID=MS.gene33644.t1;Parent=MS.gene33644 |
| chr1.1 | exon | 15072541 | 15073207 | 15072541 | ID=MS.gene33644.t1.exon1;Parent=MS.gene33644.t1 |
| chr1.1 | CDS | 15072541 | 15073207 | 15072541 | ID=cds.MS.gene33644.t1;Parent=MS.gene33644.t1 |
| chr1.1 | exon | 15073485 | 15073549 | 15073485 | ID=MS.gene33644.t1.exon2;Parent=MS.gene33644.t1 |
| chr1.1 | CDS | 15073485 | 15073549 | 15073485 | ID=cds.MS.gene33644.t1;Parent=MS.gene33644.t1 |
| Gene Sequence |
| Protein sequence |
