AlfalfaGEDB | MS.gene89104

M. sativa cultivar XinJiangDaYe / MS.gene89104

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89104.t1	XP_013465529.1	88.4	507	45	3	1	504	1	496	6.70E-158	567.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89104.t1	Q9LJG8	46.2	93	46	2	283	372	84	175	8.9e-18	93.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89104.t1	A0A072VC01	88.4	507	45	3	1	504	1	496	4.8e-158	567.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene89104.t1	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene89104	0.851981	6.23E-61	-1.69E-46
MS.gene049159	MS.gene89104	0.812143	4.92E-51	-1.69E-46
MS.gene049254	MS.gene89104	0.829245	5.73E-55	-1.69E-46
MS.gene049283	MS.gene89104	0.815639	8.34E-52	-1.69E-46
MS.gene049350	MS.gene89104	0.815852	7.48E-52	-1.69E-46
MS.gene049368	MS.gene89104	0.823015	1.74E-53	-1.69E-46
MS.gene049452	MS.gene89104	0.804034	2.62E-49	-1.69E-46
MS.gene049499	MS.gene89104	0.814353	1.61E-51	-1.69E-46
MS.gene049786	MS.gene89104	0.80722	5.62E-50	-1.69E-46
MS.gene049801	MS.gene89104	0.808578	2.89E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89104.t1	MTR_2g102227	86.117	497	55	3	11	504	11	496	0.0	721
MS.gene89104.t1	MTR_6g486270	52.252	222	101	3	282	500	129	348	1.82e-60	203
MS.gene89104.t1	MTR_8g022290	28.339	307	143	10	262	500	37	334	5.53e-20	92.0
MS.gene89104.t1	MTR_4g079960	43.617	94	47	2	283	370	31	124	8.58e-16	78.6
MS.gene89104.t1	MTR_4g079960	43.617	94	47	2	283	370	31	124	1.04e-15	78.6
MS.gene89104.t1	MTR_4g079960	43.617	94	47	2	283	370	31	124	1.67e-15	77.8
MS.gene89104.t1	MTR_2g016140	39.785	93	51	2	283	370	23	115	3.03e-15	77.0
MS.gene89104.t1	MTR_2g016140	39.785	93	51	2	283	370	23	115	5.19e-15	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89104.t1	AT3G24490	42.912	261	129	4	245	504	65	306	4.49e-59	198
MS.gene89104.t1	AT3G14180	42.857	105	53	3	283	384	84	184	3.21e-22	99.8
MS.gene89104.t1	AT1G54060	32.031	256	130	8	283	496	94	347	8.71e-19	88.6
MS.gene89104.t1	AT3G11100	32.569	218	137	3	283	496	22	233	1.17e-17	83.2
MS.gene89104.t1	AT5G05550	30.837	227	136	6	283	502	24	236	8.61e-16	77.4
MS.gene89104.t1	AT5G05550	30.837	227	136	6	283	502	24	236	1.22e-15	77.0
MS.gene89104.t1	AT3G58630	37.255	102	50	2	283	370	26	127	1.78e-15	77.8
MS.gene89104.t1	AT5G05550	30.837	227	136	6	283	502	24	236	1.83e-15	76.6
MS.gene89104.t1	AT3G58630	37.255	102	50	2	283	370	26	127	2.14e-15	77.8
MS.gene89104.t1	AT3G10030	35.556	90	57	1	282	370	160	249	1.72e-13	73.2

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Find 127 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTTGACGACGAGGAT+GGG	0.474465	2.4:+2760027	MS.gene89104:CDS
AAAGAAAAGAAGGAGAGGAA+GGG	0.537660	2.4:+2760694	MS.gene89104:CDS
AAATGAGATGGATGACATGA+AGG	0.616691	2.4:+2760613	MS.gene89104:CDS
AAATGTTGACGACGAGGATG+GGG	0.548278	2.4:+2760028	MS.gene89104:CDS
AACCATATTCCTCGCTTTGT+CGG	0.453074	2.4:-2759803	None:intergenic
AAGAAAAGAAGGAGAGGAAG+GGG	0.472304	2.4:+2760695	MS.gene89104:CDS
AAGATAGCAATGACGAGGAT+GGG	0.513429	2.4:+2759994	MS.gene89104:CDS
AAGATTAAGTTTCAAGATAT+GGG	0.423794	2.4:+2760452	MS.gene89104:CDS
AAGGAAGAGTCTTCGATCTG+AGG	0.584495	2.4:+2760322	MS.gene89104:CDS
AAGTTTCAAGATATGGGTGG+TGG	0.524195	2.4:+2760458	MS.gene89104:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAAGTTTCAAGATATGGG+TGG	+	chr2.4:2760455-2760474	MS.gene89104:CDS	30.0%
ATTTGTTCTTCTTGATGCTT+GGG	+	chr2.4:2760277-2760296	MS.gene89104:CDS	30.0%
GAATATGAAGAATTCCTCAA+AGG	-	chr2.4:2759513-2759532	None:intergenic	30.0%
GGTTTCAAAAGTTGAAAGAA+CGG	+	chr2.4:2760376-2760395	MS.gene89104:CDS	30.0%
GTTTCAAAAGTTGAAAGAAC+GGG	+	chr2.4:2760377-2760396	MS.gene89104:CDS	30.0%
TGAATGAAGATGAATTAGAG+TGG	+	chr2.4:2760117-2760136	MS.gene89104:CDS	30.0%
TGTAAATCATGCAAATGAGA+TGG	+	chr2.4:2760601-2760620	MS.gene89104:CDS	30.0%
TTGGTTATCCTAACAATCAA+GGG	+	chr2.4:2759469-2759488	MS.gene89104:CDS	30.0%
AAAGAAAAGAAGGAGAGGAA+GGG	+	chr2.4:2760694-2760713	MS.gene89104:CDS	35.0%
AAATGAGATGGATGACATGA+AGG	+	chr2.4:2760613-2760632	MS.gene89104:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	2759414	2760928	2759414	ID=MS.gene89104
chr2.4	mRNA	2759414	2760928	2759414	ID=MS.gene89104.t1;Parent=MS.gene89104
chr2.4	exon	2759414	2760928	2759414	ID=MS.gene89104.t1.exon1;Parent=MS.gene89104.t1
chr2.4	CDS	2759414	2760928	2759414	ID=cds.MS.gene89104.t1;Parent=MS.gene89104.t1

Gene Sequence

>MS.gene89104

ATGGATGACATTGATGATGATGCAATTGCAAGGTATCCCTCTAACCCATATGGTGTTGGTTATCCTAACAATCAAGGGTATGGCGATCTCAAACACCCTTTGAGGAATTCTTCATATTCCCATCCAGCTGCAAATGAATCTACCCGAGATTATGATCATGTCGATGATGAAGATCAGTTAGGAGGGAATGACGATGAAGATCGGTTAGGAGGGAATGATGATGATGTTGTTGATGAAGACCGGTTAGGAGGGAACGGTGATGATGTTGTTGATGAAGACCGGATAGGAGGGAATGATGATGTTGTTGATGATGATGATGATGTTGATCATCATTCCCTAAAGCAGGTGGATGAAGACGATGACACCGATGGGGGTGGTGATGACAATGACCGACAAAGCGAGGAATATGGTTATCTGAAACATTCTGTGAGGAATTCTCCATATTCCCAGCCATTTGGGAATGAAGGTAACAATGATGAGGATGAAGAAGATGAAGAGCGGTTAGTGGAGGAAGATGATGATGATGTTGATATTGATCAAAATAACAATGTTCAGCTTTCCCTAAAGCATGCTGATGAAGAAGATAGCAATGACGAGGATGGGGATAGGGATGAAAATGTTGACGACGAGGATGGGGATAGGGATGAAAATGTTGATGACAATGACGGTGATAATGACAATAAGCAGGAAAGTTATGCCATGATGAATGAAGATGAATTAGAGTGGCACCCAAAAAAGCAAAAGCTAAAGAGTTTGATGTCAACTTATGAATTTGCAGCTCGTGTGCCAACACCGTCAGCTGCAGCTGCTAAACGATCTTTTGGGGTTAGGAATTCCCTTACTGAATGGACTGAGAAGGAGACATTTGTTCTTCTTGATGCTTGGGGTGATCGGTTTCTGCAACATAGAAGGAAGAGTCTTCGATCTGAGGAATGGCAACAAGTTGCAGAAAAGGTATCAGTGGTTTCAAAAGTTGAAAGAACGGGTACTCAGTGTAGAAATCGTTTAGATACATTGAAGAAAAAATACAAAAAGGACAAGATTAAGTTTCAAGATATGGGTGGTGGAGATAGAAAATGGGTTTATTTTCAAAAGATGGATAAACTAATGTCTTCTCCACCACAACAAGCAAGACTCCCTTGTCGTTCGAACTCAAGAGAGCATATTTTTATGAAGCCTGGAGTTTATGTAAATCATGCAAATGAGATGGATGACATGAAGGATAGTCCTCCTGAAAATACAGAATCGGTTCGTGTAGAAGGTTTCGAAGGGCCTTGCCCAAAGAAAAGAAGGAGAGGAAGGGGAAGTGATGAGTTTTCTTCCTTCAAATTACTTGCTGATTCCATTCAGAATTTTGGTAAGATATATGAGAAGATTGAAAATAATAAAAGGCAGCAGATGATGGAACTAGAAAAGATGAGGATGGATTTCCTCAACGATTTGGAAGCACAAAAGAGACAGATTTTTGAAAGATTGCAGAGTGAGATTTTAAAAATCGAGCAAAGAGATGATTAA

Protein sequence

>MS.gene89104.t1

MDDIDDDAIARYPSNPYGVGYPNNQGYGDLKHPLRNSSYSHPAANESTRDYDHVDDEDQLGGNDDEDRLGGNDDDVVDEDRLGGNGDDVVDEDRIGGNDDVVDDDDDVDHHSLKQVDEDDDTDGGGDDNDRQSEEYGYLKHSVRNSPYSQPFGNEGNNDEDEEDEERLVEEDDDDVDIDQNNNVQLSLKHADEEDSNDEDGDRDENVDDEDGDRDENVDDNDGDNDNKQESYAMMNEDELEWHPKKQKLKSLMSTYEFAARVPTPSAAAAKRSFGVRNSLTEWTEKETFVLLDAWGDRFLQHRRKSLRSEEWQQVAEKVSVVSKVERTGTQCRNRLDTLKKKYKKDKIKFQDMGGGDRKWVYFQKMDKLMSSPPQQARLPCRSNSREHIFMKPGVYVNHANEMDDMKDSPPENTESVRVEGFEGPCPKKRRRGRGSDEFSSFKLLADSIQNFGKIYEKIENNKRQQMMELEKMRMDFLNDLEAQKRQIFERLQSEILKIEQRDD