AlfalfaGEDB | MS.gene44621

M. sativa cultivar XinJiangDaYe / MS.gene44621

KEGG: -
GO: GO:0000145 GO:0005575 GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0032991 GO:0046903 GO:0051179 GO:0051234 GO:0099023 GO:0140352
PFAM: PF03081.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44621.t1	XP_024626193.1	98.4	677	11	0	1	677	1	677	0.00E+00	1282.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44621.t1	Q9FNR3	39.9	657	355	5	10	665	8	625	1.4e-130	468.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44621.t1	A0A072TXQ3	98.4	677	11	0	1	677	1	677	0.0e+00	1282.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene44621	0.832483	9.21E-56	-1.69E-46
MS.gene049159	MS.gene44621	0.813757	2.18E-51	-1.69E-46
MS.gene049254	MS.gene44621	0.810534	1.10E-50	-1.69E-46
MS.gene049283	MS.gene44621	0.835389	1.73E-56	-1.69E-46
MS.gene049368	MS.gene44621	0.824531	7.68E-54	-1.69E-46
MS.gene049452	MS.gene44621	0.832448	9.40E-56	-1.69E-46
MS.gene049499	MS.gene44621	0.824359	8.43E-54	-1.69E-46
MS.gene049801	MS.gene44621	0.811775	5.91E-51	-1.69E-46
MS.gene049983	MS.gene44621	0.869327	3.37E-66	-1.69E-46
MS.gene050414	MS.gene44621	0.800608	1.33E-48	-1.69E-46
MS.gene050654	MS.gene44621	0.871087	8.93E-67	-1.69E-46
MS.gene050737	MS.gene44621	0.820613	6.25E-53	-1.69E-46
MS.gene050759	MS.gene44621	0.822386	2.44E-53	-1.69E-46
MS.gene050941	MS.gene44621	0.831456	1.65E-55	-1.69E-46
MS.gene050962	MS.gene44621	0.812196	4.79E-51	-1.69E-46
MS.gene051147	MS.gene44621	0.801911	7.20E-49	-1.69E-46
MS.gene051198	MS.gene44621	0.840229	9.86E-58	-1.69E-46
MS.gene051279	MS.gene44621	0.805647	1.21E-49	-1.69E-46
MS.gene051332	MS.gene44621	0.813918	2.01E-51	-1.69E-46
MS.gene051541	MS.gene44621	0.829228	5.79E-55	-1.69E-46
MS.gene051800	MS.gene44621	0.836695	8.05E-57	-1.69E-46
MS.gene052016	MS.gene44621	0.812117	4.98E-51	-1.69E-46
MS.gene052037	MS.gene44621	0.811924	5.49E-51	-1.69E-46
MS.gene052118	MS.gene44621	0.842082	3.21E-58	-1.69E-46
MS.gene052463	MS.gene44621	0.839918	1.19E-57	-1.69E-46
MS.gene052493	MS.gene44621	0.800945	1.14E-48	-1.69E-46
MS.gene052518	MS.gene44621	0.807025	6.18E-50	-1.69E-46
MS.gene052582	MS.gene44621	0.818868	1.57E-52	-1.69E-46
MS.gene052584	MS.gene44621	0.815827	7.57E-52	-1.69E-46
MS.gene052674	MS.gene44621	0.828088	1.09E-54	-1.69E-46
MS.gene052675	MS.gene44621	0.825152	5.48E-54	-1.69E-46
MS.gene052677	MS.gene44621	0.833573	4.94E-56	-1.69E-46
MS.gene053079	MS.gene44621	0.805172	1.52E-49	-1.69E-46
MS.gene05308	MS.gene44621	0.807628	4.61E-50	-1.69E-46
MS.gene05350	MS.gene44621	0.853471	2.34E-61	-1.69E-46
MS.gene05423	MS.gene44621	0.823813	1.13E-53	-1.69E-46
MS.gene054479	MS.gene44621	0.868459	6.43E-66	-1.69E-46
MS.gene054489	MS.gene44621	0.822982	1.77E-53	-1.69E-46
MS.gene05458	MS.gene44621	0.834692	2.59E-56	-1.69E-46
MS.gene054641	MS.gene44621	0.84455	7.05E-59	-1.69E-46
MS.gene054651	MS.gene44621	0.821305	4.33E-53	-1.69E-46
MS.gene054892	MS.gene44621	0.821054	4.95E-53	-1.69E-46
MS.gene054893	MS.gene44621	0.823024	1.73E-53	-1.69E-46
MS.gene055327	MS.gene44621	0.832641	8.42E-56	-1.69E-46
MS.gene055513	MS.gene44621	0.803944	2.74E-49	-1.69E-46
MS.gene055864	MS.gene44621	0.847909	8.58E-60	-1.69E-46
MS.gene055926	MS.gene44621	0.830595	2.69E-55	-1.69E-46
MS.gene056045	MS.gene44621	0.812908	3.35E-51	-1.69E-46
MS.gene056174	MS.gene44621	0.850118	2.09E-60	-1.69E-46
MS.gene056308	MS.gene44621	0.808753	2.65E-50	-1.69E-46
MS.gene056480	MS.gene44621	0.838273	3.17E-57	-1.69E-46
MS.gene056776	MS.gene44621	0.821774	3.38E-53	-1.69E-46
MS.gene05686	MS.gene44621	0.814367	1.60E-51	-1.69E-46
MS.gene056873	MS.gene44621	0.802539	5.35E-49	-1.69E-46
MS.gene05690	MS.gene44621	0.816146	6.43E-52	-1.69E-46
MS.gene056973	MS.gene44621	0.812393	4.34E-51	-1.69E-46
MS.gene057208	MS.gene44621	0.833125	6.38E-56	-1.69E-46
MS.gene057371	MS.gene44621	0.800726	1.26E-48	-1.69E-46
MS.gene057598	MS.gene44621	0.824192	9.22E-54	-1.69E-46
MS.gene057600	MS.gene44621	0.803138	4.02E-49	-1.69E-46
MS.gene057855	MS.gene44621	0.803081	4.13E-49	-1.69E-46
MS.gene057915	MS.gene44621	0.811559	6.59E-51	-1.69E-46
MS.gene057919	MS.gene44621	0.806365	8.52E-50	-1.69E-46
MS.gene058010	MS.gene44621	0.805871	1.08E-49	-1.69E-46
MS.gene058081	MS.gene44621	0.85254	4.32E-61	-1.69E-46
MS.gene058159	MS.gene44621	0.834491	2.91E-56	-1.69E-46
MS.gene058223	MS.gene44621	-0.819815	9.53E-53	-1.69E-46
MS.gene058279	MS.gene44621	0.810155	1.33E-50	-1.69E-46
MS.gene058427	MS.gene44621	0.802595	5.21E-49	-1.69E-46
MS.gene058429	MS.gene44621	0.86023	2.39E-63	-1.69E-46
MS.gene058625	MS.gene44621	0.800636	1.31E-48	-1.69E-46
MS.gene058781	MS.gene44621	0.808861	2.52E-50	-1.69E-46
MS.gene058783	MS.gene44621	0.810984	8.78E-51	-1.69E-46
MS.gene058979	MS.gene44621	0.820143	8.01E-53	-1.69E-46
MS.gene05897	MS.gene44621	0.806855	6.72E-50	-1.69E-46
MS.gene059170	MS.gene44621	0.805541	1.27E-49	-1.69E-46
MS.gene059224	MS.gene44621	0.82026	7.54E-53	-1.69E-46
MS.gene059283	MS.gene44621	0.826522	2.59E-54	-1.69E-46
MS.gene059439	MS.gene44621	0.81719	3.75E-52	-1.69E-46
MS.gene059842	MS.gene44621	0.801465	8.89E-49	-1.69E-46
MS.gene059952	MS.gene44621	0.808293	3.33E-50	-1.69E-46
MS.gene060011	MS.gene44621	0.824342	8.50E-54	-1.69E-46
MS.gene060259	MS.gene44621	0.82702	1.97E-54	-1.69E-46
MS.gene060373	MS.gene44621	0.809827	1.56E-50	-1.69E-46
MS.gene060435	MS.gene44621	0.8632	2.95E-64	-1.69E-46
MS.gene060527	MS.gene44621	0.831918	1.27E-55	-1.69E-46
MS.gene06052	MS.gene44621	0.872959	2.13E-67	-1.69E-46
MS.gene060777	MS.gene44621	0.836199	1.08E-56	-1.69E-46
MS.gene060785	MS.gene44621	0.806415	8.32E-50	-1.69E-46
MS.gene060850	MS.gene44621	0.803561	3.29E-49	-1.69E-46
MS.gene060851	MS.gene44621	0.817902	2.59E-52	-1.69E-46
MS.gene060852	MS.gene44621	0.81638	5.70E-52	-1.69E-46
MS.gene060932	MS.gene44621	0.873155	1.83E-67	-1.69E-46
MS.gene060933	MS.gene44621	0.812435	4.25E-51	-1.69E-46
MS.gene060935	MS.gene44621	0.819248	1.28E-52	-1.69E-46
MS.gene060937	MS.gene44621	0.833855	4.20E-56	-1.69E-46
MS.gene061100	MS.gene44621	0.829823	4.15E-55	-1.69E-46
MS.gene06121	MS.gene44621	0.857144	1.99E-62	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene79407	MS.gene44621	PPI
MS.gene28226	MS.gene44621	PPI
MS.gene83829	MS.gene44621	PPI
MS.gene79406	MS.gene44621	PPI
MS.gene93502	MS.gene44621	PPI
MS.gene79408	MS.gene44621	PPI
MS.gene83828	MS.gene44621	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44621.t1	MTR_7g013770	98.966	677	7	0	1	677	1	677	0.0	1378
MS.gene44621.t1	MTR_3g025900	41.291	666	343	7	13	677	1	619	2.64e-173	509
MS.gene44621.t1	MTR_4g103540	43.029	581	296	13	116	677	126	690	1.11e-137	419
MS.gene44621.t1	MTR_8g021330	38.816	608	318	14	98	666	13	605	4.76e-122	377
MS.gene44621.t1	MTR_5g073450	32.779	662	402	13	16	667	6	634	1.07e-109	345
MS.gene44621.t1	MTR_3g031130	32.579	663	388	10	16	667	6	620	3.38e-99	317
MS.gene44621.t1	MTR_1g057560	32.143	532	313	7	150	666	127	625	1.56e-88	290
MS.gene44621.t1	MTR_1g090620	31.725	539	313	8	154	666	132	641	1.72e-88	290
MS.gene44621.t1	MTR_7g026630	30.948	601	384	15	83	666	64	650	9.23e-86	283
MS.gene44621.t1	MTR_2g096230	32.369	553	333	10	117	666	67	581	1.54e-85	280
MS.gene44621.t1	MTR_6g033310	30.726	537	323	8	150	667	220	726	7.13e-83	277
MS.gene44621.t1	MTR_5g073460	35.669	471	284	8	206	669	237	695	6.84e-82	273
MS.gene44621.t1	MTR_4g062330	29.908	545	362	9	118	660	72	598	7.12e-75	253
MS.gene44621.t1	MTR_1g067480	32.054	521	327	10	151	664	100	600	1.33e-74	252
MS.gene44621.t1	MTR_1g117090	29.912	565	349	12	150	677	123	677	1.40e-73	251
MS.gene44621.t1	MTR_1g067460	31.549	523	328	11	151	664	98	599	4.09e-67	231
MS.gene44621.t1	MTR_5g073620	30.645	372	212	5	217	572	268	609	2.67e-48	183
MS.gene44621.t1	MTR_5g073620	33.714	175	112	2	498	669	898	1071	3.66e-20	96.3
MS.gene44621.t1	MTR_5g074130	27.313	454	298	9	225	670	250	679	1.63e-41	162
MS.gene44621.t1	MTR_5g073480	26.398	447	241	11	215	655	256	620	5.89e-37	147
MS.gene44621.t1	MTR_4g008260	26.519	543	311	15	157	676	174	651	1.94e-35	143
MS.gene44621.t1	MTR_5g073920	26.027	438	280	9	240	668	259	661	1.39e-34	140
MS.gene44621.t1	MTR_3g464010	26.681	476	303	14	209	669	227	671	6.51e-32	132
MS.gene44621.t1	MTR_1g009620	24.444	540	328	14	156	672	183	665	2.03e-29	124
MS.gene44621.t1	MTR_1g017910	25.721	416	283	9	237	637	251	655	3.75e-29	124
MS.gene44621.t1	MTR_5g073900	25.945	397	231	11	247	638	4	342	9.95e-28	115
MS.gene44621.t1	MTR_5g073770	32.075	212	132	2	201	400	165	376	2.59e-25	108
MS.gene44621.t1	MTR_5g093530	25.876	371	223	8	300	664	246	570	8.39e-25	109
MS.gene44621.t1	MTR_5g073780	33.143	175	113	2	498	669	53	226	5.39e-21	92.8
MS.gene44621.t1	MTR_5g073490	32.335	167	92	3	504	665	3	153	1.41e-18	83.6
MS.gene44621.t1	MTR_0229s0040	28.141	199	127	4	488	672	24	220	1.43e-16	80.1
MS.gene44621.t1	MTR_5g073960	24.434	221	146	6	187	403	213	416	3.56e-13	72.4
MS.gene44621.t1	MTR_5g073820	25.000	268	133	7	196	428	152	386	5.87e-12	68.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44621.t1	AT3G29400	48.748	679	310	9	12	677	5	658	0.0	585
MS.gene44621.t1	AT5G61010	40.183	657	353	6	10	665	8	625	2.23e-164	486
MS.gene44621.t1	AT5G61010	40.183	657	353	6	10	665	8	625	2.23e-164	486
MS.gene44621.t1	AT5G50380	42.211	597	290	12	109	677	113	682	3.38e-149	449
MS.gene44621.t1	AT3G14090	38.333	540	295	10	153	666	86	613	3.47e-118	367
MS.gene44621.t1	AT1G72470	36.438	612	323	15	101	666	30	621	4.71e-114	356
MS.gene44621.t1	AT1G54090	41.203	449	249	7	226	666	170	611	3.14e-109	343
MS.gene44621.t1	AT5G58430	31.429	665	392	12	16	667	6	619	5.61e-103	327
MS.gene44621.t1	AT5G03540	32.784	546	312	8	151	676	13	523	1.44e-90	291
MS.gene44621.t1	AT5G03540	30.396	681	391	15	24	676	13	638	1.46e-90	295
MS.gene44621.t1	AT1G07000	30.344	669	372	16	16	667	6	597	6.44e-90	292
MS.gene44621.t1	AT5G03540	30.029	686	395	14	24	666	13	656	1.04e-88	291
MS.gene44621.t1	AT5G52340	30.783	562	348	8	115	666	93	623	4.39e-86	283
MS.gene44621.t1	AT5G59730	32.616	558	352	10	118	668	60	600	3.44e-83	275
MS.gene44621.t1	AT5G59730	32.737	559	350	11	118	668	60	600	6.87e-83	275
MS.gene44621.t1	AT2G28640	30.577	520	328	6	151	669	94	581	9.62e-82	271
MS.gene44621.t1	AT5G13150	31.690	568	356	14	114	667	96	645	3.20e-81	271
MS.gene44621.t1	AT1G07725	32.563	519	324	9	151	666	96	591	5.40e-81	269
MS.gene44621.t1	AT5G13990	30.635	630	353	17	76	667	99	682	2.42e-79	266
MS.gene44621.t1	AT2G39380	29.211	558	365	7	118	667	76	611	8.91e-77	258
MS.gene44621.t1	AT3G09520	29.000	600	367	13	88	664	41	604	1.78e-70	241
MS.gene44621.t1	AT5G52350	30.684	453	284	4	221	666	148	577	3.39e-69	237
MS.gene44621.t1	AT3G09530	28.996	538	349	8	141	666	86	602	6.75e-65	226
MS.gene44621.t1	AT3G55150	28.342	561	368	9	117	667	75	611	3.50e-64	224
MS.gene44621.t1	AT2G28650	29.320	515	307	9	151	664	96	554	6.76e-59	208
MS.gene44621.t1	AT4G31540	25.056	443	303	7	232	664	231	654	1.78e-33	137
MS.gene44621.t1	AT1G51640	24.169	451	297	9	237	665	199	626	7.35e-23	103

Find 146 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCAAAAGGTTGCCTATTC+TGG	0.158452	7.3:-83823490	None:intergenic
AGACATATTGTGATGGCTTT+AGG	0.215581	7.3:+83823038	MS.gene44621:CDS
TCAATCGATGATTTGGGATT+TGG	0.227503	7.3:+83823289	MS.gene44621:CDS
GAGAAGGAGGAGGGGAAGAT+AGG	0.232008	7.3:+83823143	MS.gene44621:CDS
CAAAGATCAAAAGATGGATT+TGG	0.241414	7.3:+83823843	MS.gene44621:CDS
CTTCTCACTTTGTATGTTCA+TGG	0.251343	7.3:-83823126	None:intergenic
GTTCGCCTTCTCCGAAAATT+TGG	0.255340	7.3:-83823919	None:intergenic
GGGCTTTGAAAATCTTTGTT+AGG	0.261655	7.3:+83823864	MS.gene44621:CDS
AGGAAGATCAATCGATGATT+TGG	0.283223	7.3:+83823282	MS.gene44621:CDS
GACATATTGTGATGGCTTTA+GGG	0.283742	7.3:+83823039	MS.gene44621:CDS
TATTGAAGATGTCTTGAAAA+TGG	0.283952	7.3:+83823802	MS.gene44621:CDS
TGATTTAGAAAATTATCTTT+TGG	0.292941	7.3:+83824930	MS.gene44621:CDS
TACATGGCTGAAAAGGTTAA+AGG	0.293386	7.3:+83824544	MS.gene44621:CDS
TTCCATTCTCAACTTTCTTA+AGG	0.294818	7.3:+83824663	MS.gene44621:CDS
AGTTGTTGCAAAATCCTATT+AGG	0.296540	7.3:+83824974	MS.gene44621:CDS
CAATCGATGATTTGGGATTT+GGG	0.298646	7.3:+83823290	MS.gene44621:CDS
TTAAAGTGATTCAAGCTTAT+AGG	0.315348	7.3:+83824848	MS.gene44621:CDS
ACAACGACTTGGTTTGATTC+AGG	0.317391	7.3:+83823241	MS.gene44621:CDS
AGGAGGAGGGGAAGATAGGG+AGG	0.319656	7.3:+83823147	MS.gene44621:CDS
AAGCTTATACCATTGTTAGA+AGG	0.328404	7.3:+83823732	MS.gene44621:CDS
TCGGCTTCGAGGATATTTAC+AGG	0.329702	7.3:+83824761	MS.gene44621:CDS
TGCTATTATCATCTCCTAAT+AGG	0.331305	7.3:-83824988	None:intergenic
AAAGATCAAAAGATGGATTT+GGG	0.331630	7.3:+83823844	MS.gene44621:CDS
ACAAAACAAGCTTGACTAAC+AGG	0.350658	7.3:-83823941	None:intergenic
GAAGATAGGGAGGGAAAAGG+AGG	0.356692	7.3:+83823157	MS.gene44621:CDS
AGATTTGCGGATTTCTATAT+CGG	0.356841	7.3:+83824825	MS.gene44621:CDS
TTCAGGAGAAAATAATGAAA+TGG	0.362671	7.3:+83823258	MS.gene44621:CDS
TTTGGTGAAGCTATGTCTAT+TGG	0.365289	7.3:+83824001	MS.gene44621:CDS
AGAAGGAGGAGGGGAAGATA+GGG	0.365916	7.3:+83823144	MS.gene44621:CDS
CTCAACTTTCTTAAGGATGA+AGG	0.371916	7.3:+83824670	MS.gene44621:CDS
TGACATCAATGCTTTGTATT+CGG	0.372086	7.3:+83824090	MS.gene44621:CDS
TTCAGCTTCGCGAAGATTTG+CGG	0.372177	7.3:+83824812	MS.gene44621:CDS
GGGGAAGATAGGGAGGGAAA+AGG	0.388497	7.3:+83823154	MS.gene44621:CDS
AAGAAGATTCTGGCTGATCT+TGG	0.397682	7.3:+83823089	MS.gene44621:CDS
AATGAACAAACACTCATCTA+AGG	0.398840	7.3:-83823759	None:intergenic
GGAAGATCAATCGATGATTT+GGG	0.400737	7.3:+83823283	MS.gene44621:CDS
TTGGCTTCTGTTATCATCTT+CGG	0.401388	7.3:-83824383	None:intergenic
GGACGAGGAGGACGAGGAGG+AGG	0.403757	7.3:+83823196	MS.gene44621:CDS
CAATGGCTGTTGAGGAATCT+AGG	0.406090	7.3:+83822967	None:intergenic
TCTTCGCGAAGCTGAACATC+TGG	0.409566	7.3:-83824805	None:intergenic
GGCTTTGAAAATCTTTGTTA+GGG	0.412401	7.3:+83823865	MS.gene44621:CDS
TCAGGAGAAAATAATGAAAT+GGG	0.414181	7.3:+83823259	MS.gene44621:CDS
GTGTTTGTTCATTCTTGAAA+TGG	0.417456	7.3:+83823769	MS.gene44621:CDS
TTCGGACGAGGTTGGTTCCT+CGG	0.417915	7.3:+83824108	MS.gene44621:CDS
CGTGTCAGGTGACGATGAAA+TGG	0.419107	7.3:-83824350	None:intergenic
GAAAATGGAATGGGGTACTT+TGG	0.421152	7.3:+83823817	MS.gene44621:CDS
CCAAGACTGAAGCAAATGAT+AGG	0.421729	7.3:-83824444	None:intergenic
CAAGCTTATAGGACATTTGT+TGG	0.424622	7.3:+83824859	MS.gene44621:CDS
CCTACATTACATGGCTGAAA+AGG	0.424669	7.3:+83824537	MS.gene44621:CDS
ATTCAGTGCTTCGCTGTAAT+CGG	0.425187	7.3:-83824296	None:intergenic
ATCGTCACCTGACACGAGTC+CGG	0.427795	7.3:+83824357	MS.gene44621:CDS
AAATACTCAGACGTTTCTTC+AGG	0.429562	7.3:-83823314	None:intergenic
GAGAATTCGTATCAATTTCT+CGG	0.430289	7.3:-83824035	None:intergenic
TTCGTATCAATTTCTCGGGT+TGG	0.433899	7.3:-83824030	None:intergenic
CCTATCATTTGCTTCAGTCT+TGG	0.436027	7.3:+83824444	MS.gene44621:CDS
AGCGAGGAAGATGTTGTTGA+TGG	0.439521	7.3:+83823536	MS.gene44621:CDS
TTCTCTTCTCTTTCTAACTC+AGG	0.440235	7.3:-83822999	None:intergenic
TGTCATTGATTTAGTTCATC+CGG	0.441468	7.3:+83823640	MS.gene44621:CDS
ATCCTTAAGAAAGTTGAGAA+TGG	0.446088	7.3:-83824665	None:intergenic
CGGCTGTGATTCCTGATCTC+AGG	0.446347	7.3:+83823660	MS.gene44621:CDS
TCTGAACTTAGGATCATATT+CGG	0.456601	7.3:+83824568	MS.gene44621:CDS
GCAACCTGCTTATCCAGAAT+AGG	0.463293	7.3:+83823477	MS.gene44621:CDS
CTGTTATCATCTTCGGTACC+CGG	0.467583	7.3:-83824376	None:intergenic
AGAATTCGTATCAATTTCTC+GGG	0.468076	7.3:-83824034	None:intergenic
ACATGGCTGAAAAGGTTAAA+GGG	0.470527	7.3:+83824545	MS.gene44621:CDS
TCGGTACCCGGACTCGTGTC+AGG	0.474489	7.3:-83824364	None:intergenic
AATGGATCCGAAAACACAAC+TGG	0.475821	7.3:+83824596	MS.gene44621:CDS
AAGCTTCTACCTCGGCTTCG+AGG	0.476916	7.3:+83824750	MS.gene44621:CDS
CACGAGGTTCTGAACAGATT+GGG	0.480214	7.3:+83824145	MS.gene44621:CDS
AAGTGATGAAAAGAAGATTC+TGG	0.481173	7.3:+83823079	MS.gene44621:CDS
CCGTCAACAACGCCTTTAGT+TGG	0.482370	7.3:+83824220	MS.gene44621:CDS
TCCTGGTAGATTTCCCTCAA+TGG	0.483428	7.3:+83824411	MS.gene44621:CDS
GCGCATATTGATCGAGAAGT+TGG	0.488485	7.3:+83823355	MS.gene44621:CDS
CAATGCTTTGTATTCGGACG+AGG	0.488922	7.3:+83824096	MS.gene44621:CDS
AGATGTCTTGAAAATGGAAT+GGG	0.491461	7.3:+83823808	MS.gene44621:CDS
GATATGTTATGAATTATCTT+CGG	0.492481	7.3:+83824266	MS.gene44621:CDS
GAGACATACTCAGGTTCAAA+AGG	0.492494	7.3:-83823503	None:intergenic
TGTTCATTCTTGAAATGGAA+AGG	0.497581	7.3:+83823774	MS.gene44621:CDS
CCAACTAAAGGCGTTGTTGA+CGG	0.497916	7.3:-83824220	None:intergenic
GTTGCCTATTCTGGATAAGC+AGG	0.498736	7.3:-83823481	None:intergenic
TTGATTATCAATGGCTGTTG+AGG	0.499717	7.3:+83822959	None:intergenic
AAAGGAGGATGATATAGATA+AGG	0.499854	7.3:+83823172	MS.gene44621:CDS
CTTATAGGACATTTGTTGGA+AGG	0.502026	7.3:+83824863	MS.gene44621:CDS
CCACGAGGTTCTGAACAGAT+TGG	0.503504	7.3:+83824144	MS.gene44621:CDS
AGGAATTGCCGCGCCATTGA+GGG	0.506822	7.3:-83824424	None:intergenic
TTTACAGGATTCAAACAGCT+TGG	0.507788	7.3:+83824776	MS.gene44621:CDS
TTTGCGATACACCTGAGATC+AGG	0.508198	7.3:-83823671	None:intergenic
ATCAATTTCTCGGGTTGGTG+AGG	0.514697	7.3:-83824025	None:intergenic
TGATATAGATAAGGAGGACG+AGG	0.514775	7.3:+83823181	MS.gene44621:CDS
AGGTTAAAGGGTCTGAACTT+AGG	0.515044	7.3:+83824557	MS.gene44621:CDS
TGTATCCCTCCACCAACTAA+AGG	0.515506	7.3:-83824232	None:intergenic
GGGTTTATCTTGCAAGTGAA+AGG	0.515546	7.3:+83823885	MS.gene44621:CDS
GAGGAGGAGGAAGAAGGTGT+TGG	0.517714	7.3:+83823209	MS.gene44621:CDS
TCAACAACGCCTTTAGTTGG+TGG	0.518800	7.3:+83824223	MS.gene44621:CDS
AACAGAAGCCAAGCATCTCC+TGG	0.521198	7.3:+83824394	MS.gene44621:CDS
TCGTCACCTGACACGAGTCC+GGG	0.521282	7.3:+83824358	MS.gene44621:CDS
AGCTTATACCATTGTTAGAA+GGG	0.522929	7.3:+83823733	MS.gene44621:CDS
AATGAACAACCTACATTACA+TGG	0.524061	7.3:+83824528	MS.gene44621:CDS
GAAATCTACCAGGAGATGCT+TGG	0.524343	7.3:-83824402	None:intergenic
TGGTTTCAAAGATCAAAAGA+TGG	0.529315	7.3:+83823837	MS.gene44621:CDS
AAGATGTCTTGAAAATGGAA+TGG	0.530741	7.3:+83823807	MS.gene44621:CDS
GGAGGATGATATAGATAAGG+AGG	0.534132	7.3:+83823175	MS.gene44621:CDS
GGCTTATAGTGTTCTTCAGA+CGG	0.538239	7.3:+83823427	MS.gene44621:CDS
TCTGAAAGATCAAGAAGAAG+AGG	0.540005	7.3:+83824324	MS.gene44621:CDS
GAATACAAAGCATTGATGTC+AGG	0.541545	7.3:-83824088	None:intergenic
ACAACGCCTTTAGTTGGTGG+AGG	0.547732	7.3:+83824226	MS.gene44621:CDS
TAAGGAGGACGAGGAGGACG+AGG	0.548309	7.3:+83823190	MS.gene44621:CDS
TTTGAGACAGAATTTGAACC+AGG	0.551804	7.3:-83824703	None:intergenic
TGAAATACGAGAGAGCTAGT+TGG	0.555215	7.3:+83824638	MS.gene44621:CDS
GAGGACGAGGAGGAGGAAGA+AGG	0.558808	7.3:+83823203	MS.gene44621:CDS
TTCATAACATATCTCGCCAA+TGG	0.563562	7.3:-83824256	None:intergenic
GCCATTGAGGGAAATCTACC+AGG	0.565256	7.3:-83824412	None:intergenic
GGATCATATTCGGAGACGAA+TGG	0.566493	7.3:+83824578	MS.gene44621:CDS
GATGAAGGAATTCACGCTCC+TGG	0.569538	7.3:+83824685	MS.gene44621:CDS
GGAGGACGAGGAGGACGAGG+AGG	0.570295	7.3:+83823193	MS.gene44621:CDS
TTCTTCAGACGGCTATGACA+CGG	0.570975	7.3:+83823438	MS.gene44621:CDS
TAGGAATTGCCGCGCCATTG+AGG	0.571255	7.3:-83824425	None:intergenic
AAGTTACGAAGCTTCTACCT+CGG	0.578858	7.3:+83824742	MS.gene44621:CDS
TGTAAATATCCTCGAAGCCG+AGG	0.581914	7.3:-83824759	None:intergenic
GCTTTGTATTCGGACGAGGT+TGG	0.582361	7.3:+83824100	MS.gene44621:CDS
GGAGGAGGGGAAGATAGGGA+GGG	0.582716	7.3:+83823148	MS.gene44621:CDS
GCGAGGAAGATGTTGTTGAT+GGG	0.586419	7.3:+83823537	MS.gene44621:CDS
TGGTGGAGGGATACATCCAT+TGG	0.593262	7.3:+83824240	MS.gene44621:CDS
CATGAACATACAAAGTGAGA+AGG	0.595930	7.3:+83823127	MS.gene44621:CDS
CAACGCCTTTAGTTGGTGGA+GGG	0.597098	7.3:+83824227	MS.gene44621:CDS
GGAGAAAATAATGAAATGGG+AGG	0.598922	7.3:+83823262	MS.gene44621:CDS
GATCGAAACGAGACATACTC+AGG	0.599078	7.3:-83823512	None:intergenic
CATACAAAGTGAGAAGGAGG+AGG	0.600227	7.3:+83823133	MS.gene44621:CDS
CTGAGATCAGGAATCACAGC+CGG	0.603127	7.3:-83823659	None:intergenic
TTTATCTTGCAAGTGAAAGG+TGG	0.606242	7.3:+83823888	MS.gene44621:CDS
ATCAATGACATGCTCTTCAG+TGG	0.607832	7.3:-83823627	None:intergenic
TACAAAGTGAGAAGGAGGAG+GGG	0.610000	7.3:+83823135	MS.gene44621:CDS
GGTGTTATTGAACAACGACT+TGG	0.615375	7.3:+83823230	MS.gene44621:CDS
TAAGGCATCCCTTCTAACAA+TGG	0.621969	7.3:-83823741	None:intergenic
ATACAAAGTGAGAAGGAGGA+GGG	0.633932	7.3:+83823134	MS.gene44621:CDS
GAACATACAAAGTGAGAAGG+AGG	0.636230	7.3:+83823130	MS.gene44621:CDS
ATATTGATCGAGAAGTTGGA+AGG	0.643451	7.3:+83823359	MS.gene44621:CDS
TATAGATAAGGAGGACGAGG+AGG	0.647044	7.3:+83823184	MS.gene44621:CDS
TGTCTCGTTTCGATCGAGCG+AGG	0.647882	7.3:+83823520	MS.gene44621:CDS
GTAGATTTCCCTCAATGGCG+CGG	0.659563	7.3:+83824416	MS.gene44621:CDS
TGCAGCAAGACATATTGTGA+TGG	0.663518	7.3:+83823031	MS.gene44621:CDS
AGTCTTGGAGAACAATCTCG+AGG	0.679517	7.3:+83824459	MS.gene44621:CDS
GGTTCACTTTGAATGCCACG+AGG	0.713802	7.3:+83824129	MS.gene44621:CDS
CCAATCTGTTCAGAACCTCG+TGG	0.714380	7.3:-83824144	None:intergenic
GATGTCTTGAAAATGGAATG+GGG	0.730786	7.3:+83823809	MS.gene44621:CDS
GTGGCATTCAAAGTGAACCG+AGG	0.739746	7.3:-83824125	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGATTTAGAAAATTATCTTT+TGG	+	chr7.3:83824930-83824949	MS.gene44621:CDS	15.0%
!!	GATATGTTATGAATTATCTT+CGG	+	chr7.3:83824266-83824285	MS.gene44621:CDS	20.0%
!	TATTGAAGATGTCTTGAAAA+TGG	+	chr7.3:83823802-83823821	MS.gene44621:CDS	25.0%
!	TCAGGAGAAAATAATGAAAT+GGG	+	chr7.3:83823259-83823278	MS.gene44621:CDS	25.0%
!	TTAAAGTGATTCAAGCTTAT+AGG	+	chr7.3:83824848-83824867	MS.gene44621:CDS	25.0%
!	TTCAGGAGAAAATAATGAAA+TGG	+	chr7.3:83823258-83823277	MS.gene44621:CDS	25.0%
!!	AAAGATCAAAAGATGGATTT+GGG	+	chr7.3:83823844-83823863	MS.gene44621:CDS	25.0%
!!	TATCTTTTGGATTTCTTTGA+AGG	+	chr7.3:83824943-83824962	MS.gene44621:CDS	25.0%
	AAAGGAGGATGATATAGATA+AGG	+	chr7.3:83823172-83823191	MS.gene44621:CDS	30.0%
	AAGATGTCTTGAAAATGGAA+TGG	+	chr7.3:83823807-83823826	MS.gene44621:CDS	30.0%
	AAGCTTATACCATTGTTAGA+AGG	+	chr7.3:83823732-83823751	MS.gene44621:CDS	30.0%
	AAGTGATGAAAAGAAGATTC+TGG	+	chr7.3:83823079-83823098	MS.gene44621:CDS	30.0%
	AATGAACAAACACTCATCTA+AGG	-	chr7.3:83823762-83823781	None:intergenic	30.0%
	AATGAACAACCTACATTACA+TGG	+	chr7.3:83824528-83824547	MS.gene44621:CDS	30.0%
	AGAATTCGTATCAATTTCTC+GGG	-	chr7.3:83824037-83824056	None:intergenic	30.0%
	AGATGTCTTGAAAATGGAAT+GGG	+	chr7.3:83823808-83823827	MS.gene44621:CDS	30.0%
	AGATTTGCGGATTTCTATAT+CGG	+	chr7.3:83824825-83824844	MS.gene44621:CDS	30.0%
	AGCTTATACCATTGTTAGAA+GGG	+	chr7.3:83823733-83823752	MS.gene44621:CDS	30.0%
	AGTTGTTGCAAAATCCTATT+AGG	+	chr7.3:83824974-83824993	MS.gene44621:CDS	30.0%
	ATCCTTAAGAAAGTTGAGAA+TGG	-	chr7.3:83824668-83824687	None:intergenic	30.0%
	GAGAATTCGTATCAATTTCT+CGG	-	chr7.3:83824038-83824057	None:intergenic	30.0%
	GTGTTTGTTCATTCTTGAAA+TGG	+	chr7.3:83823769-83823788	MS.gene44621:CDS	30.0%
	TCTGAACTTAGGATCATATT+CGG	+	chr7.3:83824568-83824587	MS.gene44621:CDS	30.0%
	TGACATCAATGCTTTGTATT+CGG	+	chr7.3:83824090-83824109	MS.gene44621:CDS	30.0%
	TGGTTTCAAAGATCAAAAGA+TGG	+	chr7.3:83823837-83823856	MS.gene44621:CDS	30.0%
	TGTCTCAAAAACTCTTCTAA+AGG	+	chr7.3:83824717-83824736	MS.gene44621:CDS	30.0%
	TGTTCATTCTTGAAATGGAA+AGG	+	chr7.3:83823774-83823793	MS.gene44621:CDS	30.0%
	TTCCATTCTCAACTTTCTTA+AGG	+	chr7.3:83824663-83824682	MS.gene44621:CDS	30.0%
!	AGAGTATAAGTTTTTGCAGA+GGG	+	chr7.3:83823406-83823425	MS.gene44621:CDS	30.0%
!	ATTTTGCAGCTTCTGAATTT+TGG	+	chr7.3:83823983-83824002	MS.gene44621:CDS	30.0%
!	CAAAGATCAAAAGATGGATT+TGG	+	chr7.3:83823843-83823862	MS.gene44621:CDS	30.0%
!!	AAGAGTATAAGTTTTTGCAG+AGG	+	chr7.3:83823405-83823424	MS.gene44621:CDS	30.0%
!!	GGCTTTGAAAATCTTTGTTA+GGG	+	chr7.3:83823865-83823884	MS.gene44621:CDS	30.0%
!!	TGTCATTGATTTAGTTCATC+CGG	+	chr7.3:83823640-83823659	MS.gene44621:CDS	30.0%
!!!	TTTTGGTGATGATTCATTTG+AGG	+	chr7.3:83823574-83823593	MS.gene44621:CDS	30.0%
	AAATACTCAGACGTTTCTTC+AGG	-	chr7.3:83823317-83823336	None:intergenic	35.0%
	ACAAAACAAGCTTGACTAAC+AGG	-	chr7.3:83823944-83823963	None:intergenic	35.0%
	ACATGGCTGAAAAGGTTAAA+GGG	+	chr7.3:83824545-83824564	MS.gene44621:CDS	35.0%
	AGACATATTGTGATGGCTTT+AGG	+	chr7.3:83823038-83823057	MS.gene44621:CDS	35.0%
	AGGAAGATCAATCGATGATT+TGG	+	chr7.3:83823282-83823301	MS.gene44621:CDS	35.0%
	ATATTGATCGAGAAGTTGGA+AGG	+	chr7.3:83823359-83823378	MS.gene44621:CDS	35.0%
	CAAGCTTATAGGACATTTGT+TGG	+	chr7.3:83824859-83824878	MS.gene44621:CDS	35.0%
	CATGAACATACAAAGTGAGA+AGG	+	chr7.3:83823127-83823146	MS.gene44621:CDS	35.0%
	CTCAACTTTCTTAAGGATGA+AGG	+	chr7.3:83824670-83824689	MS.gene44621:CDS	35.0%
	CTTCTCACTTTGTATGTTCA+TGG	-	chr7.3:83823129-83823148	None:intergenic	35.0%
	GATGTCTTGAAAATGGAATG+GGG	+	chr7.3:83823809-83823828	MS.gene44621:CDS	35.0%
	GGAAGATCAATCGATGATTT+GGG	+	chr7.3:83823283-83823302	MS.gene44621:CDS	35.0%
	GGAGAAAATAATGAAATGGG+AGG	+	chr7.3:83823262-83823281	MS.gene44621:CDS	35.0%
	TACATGGCTGAAAAGGTTAA+AGG	+	chr7.3:83824544-83824563	MS.gene44621:CDS	35.0%
	TCTGAAAGATCAAGAAGAAG+AGG	+	chr7.3:83824324-83824343	MS.gene44621:CDS	35.0%
	TTCATAACATATCTCGCCAA+TGG	-	chr7.3:83824259-83824278	None:intergenic	35.0%
	TTCTCTTCTCTTTCTAACTC+AGG	-	chr7.3:83823002-83823021	None:intergenic	35.0%
	TTGGCTTCTGTTATCATCTT+CGG	-	chr7.3:83824386-83824405	None:intergenic	35.0%
	TTTACAGGATTCAAACAGCT+TGG	+	chr7.3:83824776-83824795	MS.gene44621:CDS	35.0%
	TTTATCTTGCAAGTGAAAGG+TGG	+	chr7.3:83823888-83823907	MS.gene44621:CDS	35.0%
	TTTGGTGAAGCTATGTCTAT+TGG	+	chr7.3:83824001-83824020	MS.gene44621:CDS	35.0%
!	CAATCGATGATTTGGGATTT+GGG	+	chr7.3:83823290-83823309	MS.gene44621:CDS	35.0%
!	CTTATAGGACATTTGTTGGA+AGG	+	chr7.3:83824863-83824882	MS.gene44621:CDS	35.0%
!	GAAACTTCCAGTTGTGTTTT+CGG	-	chr7.3:83824606-83824625	None:intergenic	35.0%
!	GACATATTGTGATGGCTTTA+GGG	+	chr7.3:83823039-83823058	MS.gene44621:CDS	35.0%
!	GGGAATTCTTTTGTCTCTTT+TGG	+	chr7.3:83823557-83823576	MS.gene44621:CDS	35.0%
!	TCAATCGATGATTTGGGATT+TGG	+	chr7.3:83823289-83823308	MS.gene44621:CDS	35.0%
!	TGGCTTAGTGACCAAATTTT+CGG	+	chr7.3:83823908-83823927	MS.gene44621:CDS	35.0%
!	TTTGAGACAGAATTTGAACC+AGG	-	chr7.3:83824706-83824725	None:intergenic	35.0%
!!	GAATACAAAGCATTGATGTC+AGG	-	chr7.3:83824091-83824110	None:intergenic	35.0%
!!	GGGCTTTGAAAATCTTTGTT+AGG	+	chr7.3:83823864-83823883	MS.gene44621:CDS	35.0%
	AAGTTACGAAGCTTCTACCT+CGG	+	chr7.3:83824742-83824761	MS.gene44621:CDS	40.0%
	AATGGATCCGAAAACACAAC+TGG	+	chr7.3:83824596-83824615	MS.gene44621:CDS	40.0%
	AGGTTAAAGGGTCTGAACTT+AGG	+	chr7.3:83824557-83824576	MS.gene44621:CDS	40.0%
	ATACAAAGTGAGAAGGAGGA+GGG	+	chr7.3:83823134-83823153	MS.gene44621:CDS	40.0%
	ATCAATGACATGCTCTTCAG+TGG	-	chr7.3:83823630-83823649	None:intergenic	40.0%
	ATTCAGTGCTTCGCTGTAAT+CGG	-	chr7.3:83824299-83824318	None:intergenic	40.0%
	CCAAGACTGAAGCAAATGAT+AGG	-	chr7.3:83824447-83824466	None:intergenic	40.0%
	CCTACATTACATGGCTGAAA+AGG	+	chr7.3:83824537-83824556	MS.gene44621:CDS	40.0%
	CCTATCATTTGCTTCAGTCT+TGG	+	chr7.3:83824444-83824463	MS.gene44621:CDS	40.0%
	GAACATACAAAGTGAGAAGG+AGG	+	chr7.3:83823130-83823149	MS.gene44621:CDS	40.0%
	GAGACATACTCAGGTTCAAA+AGG	-	chr7.3:83823506-83823525	None:intergenic	40.0%
	GGAGGATGATATAGATAAGG+AGG	+	chr7.3:83823175-83823194	MS.gene44621:CDS	40.0%
	GGCTTATAGTGTTCTTCAGA+CGG	+	chr7.3:83823427-83823446	MS.gene44621:CDS	40.0%
	GGGTTTATCTTGCAAGTGAA+AGG	+	chr7.3:83823885-83823904	MS.gene44621:CDS	40.0%
	GTTCAAAAGGTTGCCTATTC+TGG	-	chr7.3:83823493-83823512	None:intergenic	40.0%
	TGAAATACGAGAGAGCTAGT+TGG	+	chr7.3:83824638-83824657	MS.gene44621:CDS	40.0%
	TGATATAGATAAGGAGGACG+AGG	+	chr7.3:83823181-83823200	MS.gene44621:CDS	40.0%
	TGCAGCAAGACATATTGTGA+TGG	+	chr7.3:83823031-83823050	MS.gene44621:CDS	40.0%
	TTCGTATCAATTTCTCGGGT+TGG	-	chr7.3:83824033-83824052	None:intergenic	40.0%
!	AAGAAGATTCTGGCTGATCT+TGG	+	chr7.3:83823089-83823108	MS.gene44621:CDS	40.0%
!	AGTGACCAAATTTTCGGAGA+AGG	+	chr7.3:83823914-83823933	MS.gene44621:CDS	40.0%
!	CCTTTTCAGCCATGTAATGT+AGG	-	chr7.3:83824540-83824559	None:intergenic	40.0%
!	GAAAATGGAATGGGGTACTT+TGG	+	chr7.3:83823817-83823836	MS.gene44621:CDS	40.0%
!	TAAGGCATCCCTTCTAACAA+TGG	-	chr7.3:83823744-83823763	None:intergenic	40.0%
!!	ACAACGACTTGGTTTGATTC+AGG	+	chr7.3:83823241-83823260	MS.gene44621:CDS	40.0%
!!	GGTGTTATTGAACAACGACT+TGG	+	chr7.3:83823230-83823249	MS.gene44621:CDS	40.0%
	AGCGAGGAAGATGTTGTTGA+TGG	+	chr7.3:83823536-83823555	MS.gene44621:CDS	45.0%
	AGTCTTGGAGAACAATCTCG+AGG	+	chr7.3:83824459-83824478	MS.gene44621:CDS	45.0%
	CAATGCTTTGTATTCGGACG+AGG	+	chr7.3:83824096-83824115	MS.gene44621:CDS	45.0%
	CATACAAAGTGAGAAGGAGG+AGG	+	chr7.3:83823133-83823152	MS.gene44621:CDS	45.0%
	CTGTTATCATCTTCGGTACC+CGG	-	chr7.3:83824379-83824398	None:intergenic	45.0%
	GAAATCTACCAGGAGATGCT+TGG	-	chr7.3:83824405-83824424	None:intergenic	45.0%
	GATCGAAACGAGACATACTC+AGG	-	chr7.3:83823515-83823534	None:intergenic	45.0%
	GCAACCTGCTTATCCAGAAT+AGG	+	chr7.3:83823477-83823496	MS.gene44621:CDS	45.0%
	GCGAGGAAGATGTTGTTGAT+GGG	+	chr7.3:83823537-83823556	MS.gene44621:CDS	45.0%
	GCGCATATTGATCGAGAAGT+TGG	+	chr7.3:83823355-83823374	MS.gene44621:CDS	45.0%
	GGATCATATTCGGAGACGAA+TGG	+	chr7.3:83824578-83824597	MS.gene44621:CDS	45.0%
	GTTCGCCTTCTCCGAAAATT+TGG	-	chr7.3:83823922-83823941	None:intergenic	45.0%
	GTTGCCTATTCTGGATAAGC+AGG	-	chr7.3:83823484-83823503	None:intergenic	45.0%
	TACAAAGTGAGAAGGAGGAG+GGG	+	chr7.3:83823135-83823154	MS.gene44621:CDS	45.0%
	TATAGATAAGGAGGACGAGG+AGG	+	chr7.3:83823184-83823203	MS.gene44621:CDS	45.0%
	TCAACAACGCCTTTAGTTGG+TGG	+	chr7.3:83824223-83824242	MS.gene44621:CDS	45.0%
	TCCTGGTAGATTTCCCTCAA+TGG	+	chr7.3:83824411-83824430	MS.gene44621:CDS	45.0%
	TCGGCTTCGAGGATATTTAC+AGG	+	chr7.3:83824761-83824780	MS.gene44621:CDS	45.0%
	TGTAAATATCCTCGAAGCCG+AGG	-	chr7.3:83824762-83824781	None:intergenic	45.0%
	TGTATCCCTCCACCAACTAA+AGG	-	chr7.3:83824235-83824254	None:intergenic	45.0%
	TTCAGCTTCGCGAAGATTTG+CGG	+	chr7.3:83824812-83824831	MS.gene44621:CDS	45.0%
	TTCTTCAGACGGCTATGACA+CGG	+	chr7.3:83823438-83823457	MS.gene44621:CDS	45.0%
	TTTGCGATACACCTGAGATC+AGG	-	chr7.3:83823674-83823693	None:intergenic	45.0%
!!	ATCAATTTCTCGGGTTGGTG+AGG	-	chr7.3:83824028-83824047	None:intergenic	45.0%
!!	CACGAGGTTCTGAACAGATT+GGG	+	chr7.3:83824145-83824164	MS.gene44621:CDS	45.0%
!!	CCAACTAAAGGCGTTGTTGA+CGG	-	chr7.3:83824223-83824242	None:intergenic	45.0%
	AACAGAAGCCAAGCATCTCC+TGG	+	chr7.3:83824394-83824413	MS.gene44621:CDS	50.0%
	AGAAGGAGGAGGGGAAGATA+GGG	+	chr7.3:83823144-83823163	MS.gene44621:CDS	50.0%
	CCAATCTGTTCAGAACCTCG+TGG	-	chr7.3:83824147-83824166	None:intergenic	50.0%
	CCGTCAACAACGCCTTTAGT+TGG	+	chr7.3:83824220-83824239	MS.gene44621:CDS	50.0%
	CGTGTCAGGTGACGATGAAA+TGG	-	chr7.3:83824353-83824372	None:intergenic	50.0%
	CTGAGATCAGGAATCACAGC+CGG	-	chr7.3:83823662-83823681	None:intergenic	50.0%
	GAAGATAGGGAGGGAAAAGG+AGG	+	chr7.3:83823157-83823176	MS.gene44621:CDS	50.0%
	GATGAAGGAATTCACGCTCC+TGG	+	chr7.3:83824685-83824704	MS.gene44621:CDS	50.0%
	GCCATTGAGGGAAATCTACC+AGG	-	chr7.3:83824415-83824434	None:intergenic	50.0%
	GCTTTGTATTCGGACGAGGT+TGG	+	chr7.3:83824100-83824119	MS.gene44621:CDS	50.0%
	GGTTCACTTTGAATGCCACG+AGG	+	chr7.3:83824129-83824148	MS.gene44621:CDS	50.0%
	GTAGATTTCCCTCAATGGCG+CGG	+	chr7.3:83824416-83824435	MS.gene44621:CDS	50.0%
	GTGGCATTCAAAGTGAACCG+AGG	-	chr7.3:83824128-83824147	None:intergenic	50.0%
	TCTTCGCGAAGCTGAACATC+TGG	-	chr7.3:83824808-83824827	None:intergenic	50.0%
	TGGTGGAGGGATACATCCAT+TGG	+	chr7.3:83824240-83824259	MS.gene44621:CDS	50.0%
!!	ACAACGCCTTTAGTTGGTGG+AGG	+	chr7.3:83824226-83824245	MS.gene44621:CDS	50.0%
!!	CAACGCCTTTAGTTGGTGGA+GGG	+	chr7.3:83824227-83824246	MS.gene44621:CDS	50.0%
!!	CCACGAGGTTCTGAACAGAT+TGG	+	chr7.3:83824144-83824163	MS.gene44621:CDS	50.0%
	AAGCTTCTACCTCGGCTTCG+AGG	+	chr7.3:83824750-83824769	MS.gene44621:CDS	55.0%
	AGGAATTGCCGCGCCATTGA+GGG	-	chr7.3:83824427-83824446	None:intergenic	55.0%
	ATCGTCACCTGACACGAGTC+CGG	+	chr7.3:83824357-83824376	MS.gene44621:CDS	55.0%
	CGGCTGTGATTCCTGATCTC+AGG	+	chr7.3:83823660-83823679	MS.gene44621:CDS	55.0%
	GAGAAGGAGGAGGGGAAGAT+AGG	+	chr7.3:83823143-83823162	MS.gene44621:CDS	55.0%
	GGGGAAGATAGGGAGGGAAA+AGG	+	chr7.3:83823154-83823173	MS.gene44621:CDS	55.0%
	TAGGAATTGCCGCGCCATTG+AGG	-	chr7.3:83824428-83824447	None:intergenic	55.0%
	TGTCTCGTTTCGATCGAGCG+AGG	+	chr7.3:83823520-83823539	MS.gene44621:CDS	55.0%
!	GAGGAGGAGGAAGAAGGTGT+TGG	+	chr7.3:83823209-83823228	MS.gene44621:CDS	55.0%
!	TTCGGACGAGGTTGGTTCCT+CGG	+	chr7.3:83824108-83824127	MS.gene44621:CDS	55.0%
	AGGAGGAGGGGAAGATAGGG+AGG	+	chr7.3:83823147-83823166	MS.gene44621:CDS	60.0%
	GAGGACGAGGAGGAGGAAGA+AGG	+	chr7.3:83823203-83823222	MS.gene44621:CDS	60.0%
	GGAGGAGGGGAAGATAGGGA+GGG	+	chr7.3:83823148-83823167	MS.gene44621:CDS	60.0%
	TAAGGAGGACGAGGAGGACG+AGG	+	chr7.3:83823190-83823209	MS.gene44621:CDS	60.0%
	TCGTCACCTGACACGAGTCC+GGG	+	chr7.3:83824358-83824377	MS.gene44621:CDS	60.0%
	TCGGTACCCGGACTCGTGTC+AGG	-	chr7.3:83824367-83824386	None:intergenic	65.0%
	GGACGAGGAGGACGAGGAGG+AGG	+	chr7.3:83823196-83823215	MS.gene44621:CDS	70.0%
	GGAGGACGAGGAGGACGAGG+AGG	+	chr7.3:83823193-83823212	MS.gene44621:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	83822969	83825002	83822969	ID=MS.gene44621
chr7.3	mRNA	83822969	83825002	83822969	ID=MS.gene44621.t1;Parent=MS.gene44621
chr7.3	exon	83822969	83825002	83822969	ID=MS.gene44621.t1.exon1;Parent=MS.gene44621.t1
chr7.3	CDS	83822969	83825002	83822969	ID=cds.MS.gene44621.t1;Parent=MS.gene44621.t1

Gene Sequence

>MS.gene44621

ATGGCTGTTGAGGAATCTAGGCAAGTGATTCCTGAGTTAGAAAGAGAAGAGAATTTAATTGCTGCAGCAAGACATATTGTGATGGCTTTAGGGTCAAAGAACAGTCTTACAAGTGATGAAAAGAAGATTCTGGCTGATCTTGGATCAAAGTTGTCTTCCATGAACATACAAAGTGAGAAGGAGGAGGGGAAGATAGGGAGGGAAAAGGAGGATGATATAGATAAGGAGGACGAGGAGGACGAGGAGGAGGAAGAAGGTGTTGGTGTTATTGAACAACGACTTGGTTTGATTCAGGAGAAAATAATGAAATGGGAGGAAGATCAATCGATGATTTGGGATTTGGGACCTGAAGAAACGTCTGAGTATTTGAATGCAGCTAATGATGTGCGCATATTGATCGAGAAGTTGGAAGGTATGCATTTGAATAAAGAAGATCAAGAGTATAAGTTTTTGCAGAGGGCTTATAGTGTTCTTCAGACGGCTATGACACGGCTTGAAGAAGAGTTTAGCAACCTGCTTATCCAGAATAGGCAACCTTTTGAACCTGAGTATGTCTCGTTTCGATCGAGCGAGGAAGATGTTGTTGATGGGAATTCTTTTGTCTCTTTTGGTGATGATTCATTTGAGGAATCTCTTCGAAGAGATAGTGTCAGTCGAGCCACTGAAGAGCATGTCATTGATTTAGTTCATCCGGCTGTGATTCCTGATCTCAGGTGTATCGCAAACTTGCTTTTTGCTTCAAATTATGCTCAAGAATGTTCACAAGCTTATACCATTGTTAGAAGGGATGCCTTAGATGAGTGTTTGTTCATTCTTGAAATGGAAAGGCTAAGTATTGAAGATGTCTTGAAAATGGAATGGGGTACTTTGGTTTCAAAGATCAAAAGATGGATTTGGGCTTTGAAAATCTTTGTTAGGGTTTATCTTGCAAGTGAAAGGTGGCTTAGTGACCAAATTTTCGGAGAAGGCGAACCTGTTAGTCAAGCTTGTTTTGTCGATGCATCAAAAGCTTCAATTTTGCAGCTTCTGAATTTTGGTGAAGCTATGTCTATTGGTCCTCACCAACCCGAGAAATTGATACGAATTCTCGACATGTATGAAGTTCTTGCAGATCTTATGCCTGACATCAATGCTTTGTATTCGGACGAGGTTGGTTCCTCGGTTCACTTTGAATGCCACGAGGTTCTGAACAGATTGGGAGATTGTGTAAAGAAAACATTTCTTGAATTCAAAAATGCTGTTGATTTGAATCCGTCAACAACGCCTTTAGTTGGTGGAGGGATACATCCATTGGCGAGATATGTTATGAATTATCTTCGGACTCTTACCGATTACAGCGAAGCACTGAATCATCTTCTGAAAGATCAAGAAGAAGAGGATTCCATTTCATCGTCACCTGACACGAGTCCGGGTACCGAAGATGATAACAGAAGCCAAGCATCTCCTGGTAGATTTCCCTCAATGGCGCGGCAATTCCTATCATTTGCTTCAGTCTTGGAGAACAATCTCGAGGAGAAATCGAAGTTATACAAAGAGACTTCATTGCAGCACTTGTTCTTAATGAACAACCTACATTACATGGCTGAAAAGGTTAAAGGGTCTGAACTTAGGATCATATTCGGAGACGAATGGATCCGAAAACACAACTGGAAGTTTCAGCAGCATGCGATGAAATACGAGAGAGCTAGTTGGAGTTCCATTCTCAACTTTCTTAAGGATGAAGGAATTCACGCTCCTGGTTCAAATTCTGTCTCAAAAACTCTTCTAAAGGAGAAGTTACGAAGCTTCTACCTCGGCTTCGAGGATATTTACAGGATTCAAACAGCTTGGTCGATTCCAGATGTTCAGCTTCGCGAAGATTTGCGGATTTCTATATCGGTTAAAGTGATTCAAGCTTATAGGACATTTGTTGGAAGGAATAATAGTCATGTGAGTGATAAACATATTAGATACACTGCTGATGATTTAGAAAATTATCTTTTGGATTTCTTTGAAGGTTCTCAAAAGTTGTTGCAAAATCCTATTAGGAGATGA

Protein sequence

>MS.gene44621.t1

MAVEESRQVIPELEREENLIAAARHIVMALGSKNSLTSDEKKILADLGSKLSSMNIQSEKEEGKIGREKEDDIDKEDEEDEEEEEGVGVIEQRLGLIQEKIMKWEEDQSMIWDLGPEETSEYLNAANDVRILIEKLEGMHLNKEDQEYKFLQRAYSVLQTAMTRLEEEFSNLLIQNRQPFEPEYVSFRSSEEDVVDGNSFVSFGDDSFEESLRRDSVSRATEEHVIDLVHPAVIPDLRCIANLLFASNYAQECSQAYTIVRRDALDECLFILEMERLSIEDVLKMEWGTLVSKIKRWIWALKIFVRVYLASERWLSDQIFGEGEPVSQACFVDASKASILQLLNFGEAMSIGPHQPEKLIRILDMYEVLADLMPDINALYSDEVGSSVHFECHEVLNRLGDCVKKTFLEFKNAVDLNPSTTPLVGGGIHPLARYVMNYLRTLTDYSEALNHLLKDQEEEDSISSSPDTSPGTEDDNRSQASPGRFPSMARQFLSFASVLENNLEEKSKLYKETSLQHLFLMNNLHYMAEKVKGSELRIIFGDEWIRKHNWKFQQHAMKYERASWSSILNFLKDEGIHAPGSNSVSKTLLKEKLRSFYLGFEDIYRIQTAWSIPDVQLREDLRISISVKVIQAYRTFVGRNNSHVSDKHIRYTADDLENYLLDFFEGSQKLLQNPIRR