Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31855.t1 | GAU34592.1 | 94 | 50 | 3 | 0 | 1 | 50 | 1 | 50 | 4.60E-19 | 102.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31855.t1 | A0A2Z6ND44 | 94.0 | 50 | 3 | 0 | 1 | 50 | 1 | 50 | 3.3e-19 | 102.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene31855 | 0.817751 | 2.80E-52 | -1.69E-46 |
| MS.gene04920 | MS.gene31855 | 0.823461 | 1.37E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene31855 | 0.848849 | 4.72E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene31855 | 0.83403 | 3.79E-56 | -1.69E-46 |
| MS.gene049404 | MS.gene31855 | 0.803984 | 2.69E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene31855 | -0.823407 | 1.41E-53 | -1.69E-46 |
| MS.gene049452 | MS.gene31855 | 0.853643 | 2.09E-61 | -1.69E-46 |
| MS.gene049499 | MS.gene31855 | 0.838147 | 3.42E-57 | -1.69E-46 |
| MS.gene049669 | MS.gene31855 | 0.827519 | 1.50E-54 | -1.69E-46 |
| MS.gene049873 | MS.gene31855 | 0.805526 | 1.28E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene31855 | 0.834668 | 2.62E-56 | -1.69E-46 |
| MS.gene050026 | MS.gene31855 | 0.852721 | 3.83E-61 | -1.69E-46 |
| MS.gene050043 | MS.gene31855 | 0.810872 | 9.29E-51 | -1.69E-46 |
| MS.gene050105 | MS.gene31855 | 0.815571 | 8.64E-52 | -1.69E-46 |
| MS.gene050312 | MS.gene31855 | 0.819926 | 8.98E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene31855 | 0.823837 | 1.12E-53 | -1.69E-46 |
| MS.gene050559 | MS.gene31855 | 0.842477 | 2.53E-58 | -1.69E-46 |
| MS.gene050561 | MS.gene31855 | 0.8385 | 2.77E-57 | -1.69E-46 |
| MS.gene050562 | MS.gene31855 | 0.827033 | 1.96E-54 | -1.69E-46 |
| MS.gene050750 | MS.gene31855 | 0.830431 | 2.95E-55 | -1.69E-46 |
| MS.gene050759 | MS.gene31855 | 0.803236 | 3.84E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene31855 | 0.842919 | 1.93E-58 | -1.69E-46 |
| MS.gene051181 | MS.gene31855 | 0.821996 | 3.00E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene31855 | 0.804333 | 2.27E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene31855 | 0.838689 | 2.48E-57 | -1.69E-46 |
| MS.gene051744 | MS.gene31855 | 0.802665 | 5.04E-49 | -1.69E-46 |
| MS.gene051924 | MS.gene31855 | -0.803537 | 3.33E-49 | -1.69E-46 |
| MS.gene051926 | MS.gene31855 | -0.810825 | 9.51E-51 | -1.69E-46 |
| MS.gene052108 | MS.gene31855 | 0.825786 | 3.88E-54 | -1.69E-46 |
| MS.gene052208 | MS.gene31855 | 0.833786 | 4.37E-56 | -1.69E-46 |
| MS.gene052333 | MS.gene31855 | 0.842722 | 2.17E-58 | -1.69E-46 |
| MS.gene052500 | MS.gene31855 | 0.829916 | 3.94E-55 | -1.69E-46 |
| MS.gene052502 | MS.gene31855 | 0.826005 | 3.44E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene31855 | 0.846342 | 2.31E-59 | -1.69E-46 |
| MS.gene052534 | MS.gene31855 | 0.829107 | 6.19E-55 | -1.69E-46 |
| MS.gene052582 | MS.gene31855 | 0.820906 | 5.35E-53 | -1.69E-46 |
| MS.gene052584 | MS.gene31855 | 0.821776 | 3.37E-53 | -1.69E-46 |
| MS.gene052586 | MS.gene31855 | 0.837146 | 6.17E-57 | -1.69E-46 |
| MS.gene052674 | MS.gene31855 | 0.814115 | 1.82E-51 | -1.69E-46 |
| MS.gene052675 | MS.gene31855 | 0.814138 | 1.79E-51 | -1.69E-46 |
| MS.gene052677 | MS.gene31855 | 0.818404 | 2.00E-52 | -1.69E-46 |
| MS.gene052685 | MS.gene31855 | 0.851932 | 6.43E-61 | -1.69E-46 |
| MS.gene052773 | MS.gene31855 | 0.801637 | 8.20E-49 | -1.69E-46 |
| MS.gene052826 | MS.gene31855 | 0.830139 | 3.47E-55 | -1.69E-46 |
| MS.gene052867 | MS.gene31855 | 0.817269 | 3.60E-52 | -1.69E-46 |
| MS.gene052954 | MS.gene31855 | 0.830696 | 2.54E-55 | -1.69E-46 |
| MS.gene052965 | MS.gene31855 | -0.827592 | 1.44E-54 | -1.69E-46 |
| MS.gene053006 | MS.gene31855 | 0.848392 | 6.31E-60 | -1.69E-46 |
| MS.gene053082 | MS.gene31855 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene05308 | MS.gene31855 | 0.81721 | 3.71E-52 | -1.69E-46 |
| MS.gene053094 | MS.gene31855 | 0.805656 | 1.20E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene31855 | 0.824384 | 8.31E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene31855 | 0.81572 | 8.00E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene31855 | 0.828833 | 7.22E-55 | -1.69E-46 |
| MS.gene053321 | MS.gene31855 | 0.819227 | 1.30E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene31855 | 0.839766 | 1.30E-57 | -1.69E-46 |
| MS.gene053963 | MS.gene31855 | 0.865009 | 8.05E-65 | -1.69E-46 |
| MS.gene053995 | MS.gene31855 | 0.812817 | 3.50E-51 | -1.69E-46 |
| MS.gene05414 | MS.gene31855 | 0.821505 | 3.90E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene31855 | 0.841057 | 5.99E-58 | -1.69E-46 |
| MS.gene054222 | MS.gene31855 | 0.830835 | 2.35E-55 | -1.69E-46 |
| MS.gene054236 | MS.gene31855 | 0.837729 | 4.38E-57 | -1.69E-46 |
| MS.gene05423 | MS.gene31855 | 0.808831 | 2.55E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene31855 | -0.812225 | 4.72E-51 | -1.69E-46 |
| MS.gene05439 | MS.gene31855 | 0.814419 | 1.56E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene31855 | 0.838732 | 2.41E-57 | -1.69E-46 |
| MS.gene054479 | MS.gene31855 | 0.835748 | 1.40E-56 | -1.69E-46 |
| MS.gene054576 | MS.gene31855 | 0.814549 | 1.46E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene31855 | 0.853195 | 2.81E-61 | -1.69E-46 |
| MS.gene054775 | MS.gene31855 | 0.807033 | 6.16E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene31855 | 0.818502 | 1.90E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene31855 | 0.822317 | 2.53E-53 | -1.69E-46 |
| MS.gene054957 | MS.gene31855 | 0.801941 | 7.10E-49 | -1.69E-46 |
| MS.gene055166 | MS.gene31855 | 0.802142 | 6.46E-49 | -1.69E-46 |
| MS.gene055222 | MS.gene31855 | 0.825727 | 4.00E-54 | -1.69E-46 |
| MS.gene055228 | MS.gene31855 | 0.813 | 3.19E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene31855 | 0.827189 | 1.79E-54 | -1.69E-46 |
| MS.gene055586 | MS.gene31855 | 0.817441 | 3.29E-52 | -1.69E-46 |
| MS.gene055589 | MS.gene31855 | 0.816638 | 4.99E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene31855 | 0.826262 | 2.99E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene31855 | 0.8076 | 4.67E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene31855 | 0.8297 | 4.45E-55 | -1.69E-46 |
| MS.gene055841 | MS.gene31855 | 0.842543 | 2.43E-58 | -1.69E-46 |
| MS.gene056174 | MS.gene31855 | 0.830952 | 2.20E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene31855 | 0.803011 | 4.27E-49 | -1.69E-46 |
| MS.gene056252 | MS.gene31855 | 0.800002 | 1.77E-48 | -1.69E-46 |
| MS.gene056272 | MS.gene31855 | 0.82777 | 1.30E-54 | -1.69E-46 |
| MS.gene056366 | MS.gene31855 | 0.811452 | 6.95E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene31855 | 0.826877 | 2.13E-54 | -1.69E-46 |
| MS.gene05659 | MS.gene31855 | 0.802684 | 4.99E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene31855 | 0.840443 | 8.67E-58 | -1.69E-46 |
| MS.gene056779 | MS.gene31855 | 0.852749 | 3.76E-61 | -1.69E-46 |
| MS.gene05686 | MS.gene31855 | 0.841403 | 4.85E-58 | -1.69E-46 |
| MS.gene05690 | MS.gene31855 | 0.804062 | 2.59E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene31855 | 0.807921 | 3.99E-50 | -1.69E-46 |
| MS.gene057071 | MS.gene31855 | 0.808327 | 3.27E-50 | -1.69E-46 |
| MS.gene057155 | MS.gene31855 | 0.812084 | 5.06E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene31855 | 0.864555 | 1.12E-64 | -1.69E-46 |
| MS.gene057598 | MS.gene31855 | 0.801581 | 8.42E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene31855 | 0.810736 | 9.94E-51 | -1.69E-46 |
| MS.gene058008 | MS.gene31855 | 0.831246 | 1.86E-55 | -1.69E-46 |
| MS.gene058009 | MS.gene31855 | 0.831715 | 1.43E-55 | -1.69E-46 |
| MS.gene058037 | MS.gene31855 | 0.8489 | 4.56E-60 | -1.69E-46 |
| MS.gene058159 | MS.gene31855 | 0.831727 | 1.42E-55 | -1.69E-46 |
| MS.gene058223 | MS.gene31855 | -0.814975 | 1.17E-51 | -1.69E-46 |
| MS.gene058279 | MS.gene31855 | 0.83254 | 8.92E-56 | -1.69E-46 |
| MS.gene058548 | MS.gene31855 | 0.831913 | 1.27E-55 | -1.69E-46 |
| MS.gene058625 | MS.gene31855 | 0.812585 | 3.94E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene31855 | 0.827458 | 1.55E-54 | -1.69E-46 |
| MS.gene058783 | MS.gene31855 | 0.84658 | 1.99E-59 | -1.69E-46 |
| MS.gene058897 | MS.gene31855 | 0.810091 | 1.37E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene31855 | 0.842054 | 3.27E-58 | -1.69E-46 |
| MS.gene059051 | MS.gene31855 | 0.808464 | 3.06E-50 | -1.69E-46 |
| MS.gene059224 | MS.gene31855 | 0.800237 | 1.58E-48 | -1.69E-46 |
| MS.gene059272 | MS.gene31855 | 0.804779 | 1.83E-49 | -1.69E-46 |
| MS.gene05950 | MS.gene31855 | 0.803314 | 3.70E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene31855 | 0.841565 | 4.40E-58 | -1.69E-46 |
| MS.gene05954 | MS.gene31855 | 0.830912 | 2.25E-55 | -1.69E-46 |
| MS.gene05979 | MS.gene31855 | 0.814376 | 1.59E-51 | -1.69E-46 |
| MS.gene059830 | MS.gene31855 | 0.805639 | 1.21E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene31855 | -0.812132 | 4.94E-51 | -1.69E-46 |
| MS.gene059935 | MS.gene31855 | 0.810835 | 9.46E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene31855 | 0.825175 | 5.41E-54 | -1.69E-46 |
| MS.gene060012 | MS.gene31855 | 0.820092 | 8.23E-53 | -1.69E-46 |
| MS.gene060310 | MS.gene31855 | 0.807558 | 4.77E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene31855 | 0.802123 | 6.51E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene31855 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene31855 | 0.813367 | 2.65E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene31855 | 0.814654 | 1.38E-51 | -1.69E-46 |
| MS.gene060527 | MS.gene31855 | 0.805294 | 1.43E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene31855 | 0.809739 | 1.63E-50 | -1.69E-46 |
| MS.gene060840 | MS.gene31855 | 0.817718 | 2.85E-52 | -1.69E-46 |
| MS.gene060879 | MS.gene31855 | 0.8415 | 4.58E-58 | -1.69E-46 |
| MS.gene060932 | MS.gene31855 | 0.804709 | 1.90E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene31855 | 0.837374 | 5.40E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene31855 | 0.829282 | 5.62E-55 | -1.69E-46 |
| MS.gene061040 | MS.gene31855 | -0.823185 | 1.59E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31855.t1 | MTR_1g029440 | 97.436 | 39 | 1 | 0 | 12 | 50 | 295 | 333 | 7.14e-22 | 85.1 |
| MS.gene31855.t1 | MTR_3g088975 | 76.923 | 39 | 9 | 0 | 12 | 50 | 294 | 332 | 1.92e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCATCTTGAAGTTTATCT+TGG | 0.335721 | 1.4:+14512608 | MS.gene31855:CDS |
| GTGAATGGAAGATGGTGTTT+TGG | 0.364119 | 1.4:+14512443 | None:intergenic |
| GAATATTGATAAATCTGCTT+TGG | 0.436731 | 1.4:+14512583 | MS.gene31855:CDS |
| TTTGGTTATGTGGACAGAAC+AGG | 0.440866 | 1.4:+14512461 | MS.gene31855:CDS |
| GACGGCTAAGGATGTTCAAT+TGG | 0.451421 | 1.4:+14512646 | MS.gene31855:CDS |
| TGAACATCCTTAGCCGTCAA+TGG | 0.485117 | 1.4:-14512641 | None:intergenic |
| TGGAGAGTATGTTCCATTGA+CGG | 0.507206 | 1.4:+14512628 | MS.gene31855:CDS |
| GTATGTTCCATTGACGGCTA+AGG | 0.550284 | 1.4:+14512634 | MS.gene31855:CDS |
| TAGTGGTTGTGAATGGAAGA+TGG | 0.630102 | 1.4:+14512435 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAATATTGATAAATCTGCTT+TGG | + | chr1.4:14512583-14512602 | MS.gene31855:CDS | 25.0% |
| !!! | TTGTCTTGTTTGTTTTTGTT+TGG | + | chr1.4:14512509-14512528 | MS.gene31855:intron | 25.0% |
| GATCATCTTGAAGTTTATCT+TGG | + | chr1.4:14512608-14512627 | MS.gene31855:CDS | 30.0% | |
| ! | TTTGCAAACTGTGCTTTATT+TGG | + | chr1.4:14512538-14512557 | MS.gene31855:intron | 30.0% |
| !!! | AAGATGGTGTTTTGGTTATG+TGG | + | chr1.4:14512451-14512470 | MS.gene31855:CDS | 35.0% |
| ATGTGGACAGAACAGGTTAT+GGG | + | chr1.4:14512468-14512487 | MS.gene31855:intron | 40.0% | |
| TATGTGGACAGAACAGGTTA+TGG | + | chr1.4:14512467-14512486 | MS.gene31855:intron | 40.0% | |
| TTTGGTTATGTGGACAGAAC+AGG | + | chr1.4:14512461-14512480 | MS.gene31855:CDS | 40.0% | |
| ! | TGGAGAGTATGTTCCATTGA+CGG | + | chr1.4:14512628-14512647 | MS.gene31855:CDS | 40.0% |
| GACGGCTAAGGATGTTCAAT+TGG | + | chr1.4:14512646-14512665 | MS.gene31855:CDS | 45.0% | |
| GTATGTTCCATTGACGGCTA+AGG | + | chr1.4:14512634-14512653 | MS.gene31855:CDS | 45.0% | |
| TGAACATCCTTAGCCGTCAA+TGG | - | chr1.4:14512644-14512663 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 14512447 | 14512685 | 14512447 | ID=MS.gene31855 |
| chr1.4 | mRNA | 14512447 | 14512685 | 14512447 | ID=MS.gene31855.t1;Parent=MS.gene31855 |
| chr1.4 | exon | 14512447 | 14512482 | 14512447 | ID=MS.gene31855.t1.exon1;Parent=MS.gene31855.t1 |
| chr1.4 | CDS | 14512447 | 14512482 | 14512447 | ID=cds.MS.gene31855.t1;Parent=MS.gene31855.t1 |
| chr1.4 | exon | 14512569 | 14512685 | 14512569 | ID=MS.gene31855.t1.exon2;Parent=MS.gene31855.t1 |
| chr1.4 | CDS | 14512569 | 14512685 | 14512569 | ID=cds.MS.gene31855.t1;Parent=MS.gene31855.t1 |
| Gene Sequence |
| Protein sequence |
