AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33651


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33651.t1 MTR_1g029440 97.436 39 1 0 12 50 295 333 7.14e-22 85.1
MS.gene33651.t1 MTR_3g088975 76.923 39 9 0 12 50 294 332 1.92e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCATCTTGAAGTTTATCT+TGG 0.335721 1.1:+15000312 MS.gene33651:CDS
GTGAATGGAAGATGGTGTTT+TGG 0.364119 1.1:+15000147 None:intergenic
GAATATTGATAAATCTGCTT+TGG 0.436731 1.1:+15000287 MS.gene33651:CDS
TTTGGTTATGTGGACAGAAC+AGG 0.440866 1.1:+15000165 MS.gene33651:CDS
GACGGCTAAGGATGTTCAAT+TGG 0.451421 1.1:+15000350 MS.gene33651:CDS
TGAACATCCTTAGCCGTCAA+TGG 0.485117 1.1:-15000345 None:intergenic
TGGAGAGTACGTTCCATTGA+CGG 0.487214 1.1:+15000332 MS.gene33651:CDS
GTACGTTCCATTGACGGCTA+AGG 0.558474 1.1:+15000338 MS.gene33651:CDS
TAGTGGTTGTGAATGGAAGA+TGG 0.630102 1.1:+15000139 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATATTGATAAATCTGCTT+TGG + chr1.1:15000287-15000306 MS.gene33651:CDS 25.0%
!!! TTGTCTTGTTTGTTTTTGTT+TGG + chr1.1:15000213-15000232 MS.gene33651:intron 25.0%
GATCATCTTGAAGTTTATCT+TGG + chr1.1:15000312-15000331 MS.gene33651:CDS 30.0%
! TTTGCAAACTGTGCTTTATT+TGG + chr1.1:15000242-15000261 MS.gene33651:intron 30.0%
!!! AAGATGGTGTTTTGGTTATG+TGG + chr1.1:15000155-15000174 MS.gene33651:CDS 35.0%
ATGTGGACAGAACAGGTTAT+GGG + chr1.1:15000172-15000191 MS.gene33651:intron 40.0%
TATGTGGACAGAACAGGTTA+TGG + chr1.1:15000171-15000190 MS.gene33651:intron 40.0%
TTTGGTTATGTGGACAGAAC+AGG + chr1.1:15000165-15000184 MS.gene33651:CDS 40.0%
GACGGCTAAGGATGTTCAAT+TGG + chr1.1:15000350-15000369 MS.gene33651:CDS 45.0%
TGAACATCCTTAGCCGTCAA+TGG - chr1.1:15000348-15000367 None:intergenic 45.0%
! TGGAGAGTACGTTCCATTGA+CGG + chr1.1:15000332-15000351 MS.gene33651:CDS 45.0%
GTACGTTCCATTGACGGCTA+AGG + chr1.1:15000338-15000357 MS.gene33651:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 15000151 15000389 15000151 ID=MS.gene33651
chr1.1 mRNA 15000151 15000389 15000151 ID=MS.gene33651.t1;Parent=MS.gene33651
chr1.1 exon 15000151 15000186 15000151 ID=MS.gene33651.t1.exon1;Parent=MS.gene33651.t1
chr1.1 CDS 15000151 15000186 15000151 ID=cds.MS.gene33651.t1;Parent=MS.gene33651.t1
chr1.1 exon 15000273 15000389 15000273 ID=MS.gene33651.t1.exon2;Parent=MS.gene33651.t1
chr1.1 CDS 15000273 15000389 15000273 ID=cds.MS.gene33651.t1;Parent=MS.gene33651.t1
Gene Sequence

>MS.gene33651

ATGGAAGATGGTGTTTTGGTTATGTGGACAGAACAGGTTATGGGATACTATGTTTCTTGTTGTTGTCTTGTTTGTTTTTGTTTGGACTTGTTTTGCAAACTGTGCTTTATTTGGTTTGCAAGAGTTATCATCATGAGAATATTGATAAATCTGCTTTGGCTGATCATCTTGAAGTTTATCTTGGAGAGTACGTTCCATTGACGGCTAAGGATGTTCAATTGGAGAATTATCGTGTTTGA

Protein sequence

>MS.gene33651.t1

MEDGVLVMWTEQSYHHENIDKSALADHLEVYLGEYVPLTAKDVQLENYRV