Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53537.t1 | XP_003590164.1 | 96.2 | 80 | 3 | 0 | 1 | 80 | 1 | 80 | 8.00E-37 | 162.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53537.t1 | G7I6N1 | 96.2 | 80 | 3 | 0 | 1 | 80 | 1 | 80 | 5.7e-37 | 162.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene53537 | 0.80224 | 6.16E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene53537 | 0.804873 | 1.75E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene53537 | 0.836344 | 9.89E-57 | -1.69E-46 |
| MS.gene049283 | MS.gene53537 | 0.830481 | 2.87E-55 | -1.69E-46 |
| MS.gene049419 | MS.gene53537 | -0.819892 | 9.15E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene53537 | 0.841663 | 4.14E-58 | -1.69E-46 |
| MS.gene049983 | MS.gene53537 | 0.826529 | 2.58E-54 | -1.69E-46 |
| MS.gene050105 | MS.gene53537 | 0.809118 | 2.22E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene53537 | 0.809427 | 1.90E-50 | -1.69E-46 |
| MS.gene050561 | MS.gene53537 | 0.802834 | 4.65E-49 | -1.69E-46 |
| MS.gene050562 | MS.gene53537 | 0.80389 | 2.81E-49 | -1.69E-46 |
| MS.gene051597 | MS.gene53537 | 0.810798 | 9.64E-51 | -1.69E-46 |
| MS.gene051699 | MS.gene53537 | 0.800591 | 1.34E-48 | -1.69E-46 |
| MS.gene051832 | MS.gene53537 | 0.807732 | 4.38E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene53537 | 0.807216 | 5.63E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene53537 | 0.802459 | 5.55E-49 | -1.69E-46 |
| MS.gene052685 | MS.gene53537 | 0.822198 | 2.69E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene53537 | 0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene53537 | 0.819663 | 1.03E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene53537 | 0.817917 | 2.57E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene53537 | 0.818565 | 1.83E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene53537 | 0.853222 | 2.76E-61 | -1.69E-46 |
| MS.gene054222 | MS.gene53537 | 0.824865 | 6.40E-54 | -1.69E-46 |
| MS.gene054479 | MS.gene53537 | 0.825858 | 3.73E-54 | -1.69E-46 |
| MS.gene054576 | MS.gene53537 | 0.815878 | 7.38E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene53537 | 0.825928 | 3.59E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene53537 | 0.828004 | 1.14E-54 | -1.69E-46 |
| MS.gene054957 | MS.gene53537 | 0.806056 | 9.90E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene53537 | 0.805115 | 1.56E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene53537 | 0.807182 | 5.73E-50 | -1.69E-46 |
| MS.gene055801 | MS.gene53537 | 0.813599 | 2.36E-51 | -1.69E-46 |
| MS.gene055841 | MS.gene53537 | 0.811026 | 8.60E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene53537 | 0.810732 | 9.96E-51 | -1.69E-46 |
| MS.gene056779 | MS.gene53537 | 0.843706 | 1.19E-58 | -1.69E-46 |
| MS.gene05686 | MS.gene53537 | 0.816381 | 5.69E-52 | -1.69E-46 |
| MS.gene056986 | MS.gene53537 | 0.819204 | 1.31E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene53537 | 0.822237 | 2.64E-53 | -1.69E-46 |
| MS.gene057155 | MS.gene53537 | 0.814036 | 1.89E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene53537 | 0.839114 | 1.92E-57 | -1.69E-46 |
| MS.gene058008 | MS.gene53537 | 0.802837 | 4.64E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene53537 | 0.836612 | 8.45E-57 | -1.69E-46 |
| MS.gene058223 | MS.gene53537 | -0.805184 | 1.51E-49 | -1.69E-46 |
| MS.gene058279 | MS.gene53537 | 0.840986 | 6.25E-58 | -1.69E-46 |
| MS.gene059051 | MS.gene53537 | 0.805048 | 1.61E-49 | -1.69E-46 |
| MS.gene059224 | MS.gene53537 | 0.802145 | 6.45E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene53537 | 0.826949 | 2.05E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene53537 | -0.819573 | 1.08E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene53537 | 0.809302 | 2.02E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene53537 | 0.806478 | 8.07E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene53537 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene060840 | MS.gene53537 | 0.813408 | 2.60E-51 | -1.69E-46 |
| MS.gene060851 | MS.gene53537 | 0.804843 | 1.78E-49 | -1.69E-46 |
| MS.gene060852 | MS.gene53537 | 0.803562 | 3.29E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene53537 | 0.802108 | 6.56E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene53537 | 0.818989 | 1.47E-52 | -1.69E-46 |
| MS.gene060935 | MS.gene53537 | 0.829639 | 4.60E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene53537 | 0.834404 | 3.06E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53537.t1 | MTR_1g045370 | 96.250 | 80 | 3 | 0 | 1 | 80 | 1 | 80 | 2.84e-53 | 160 |
| MS.gene53537.t1 | MTR_1g073660 | 52.381 | 84 | 36 | 2 | 2 | 81 | 3 | 86 | 9.61e-20 | 75.9 |
| MS.gene53537.t1 | MTR_1g073660 | 51.807 | 83 | 36 | 2 | 2 | 80 | 4 | 86 | 7.23e-19 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53537.t1 | AT2G33390 | 55.556 | 54 | 24 | 0 | 27 | 80 | 26 | 79 | 4.06e-16 | 67.0 |
| MS.gene53537.t1 | AT2G33390 | 48.387 | 62 | 32 | 0 | 19 | 80 | 18 | 79 | 4.88e-16 | 67.0 |
Find 24 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGATTCAAAGGGTTCTA+AGG | 0.307183 | 1.2:+28020952 | None:intergenic |
| CTAATTCTTTGTTGCCTCCT+AGG | 0.338107 | 1.2:+28021071 | MS.gene53537:CDS |
| ACCTGTGGTGGTTTGTGTTA+TGG | 0.353770 | 1.2:+28021000 | MS.gene53537:CDS |
| GGTTGTATGTCAAGAAATTC+TGG | 0.367247 | 1.2:+28021097 | MS.gene53537:CDS |
| AATATTGTTGATGGATTCAA+AGG | 0.408359 | 1.2:+28020943 | None:intergenic |
| GAAATTCTGGTAAGACAAGA+AGG | 0.514903 | 1.2:+28021110 | MS.gene53537:CDS |
| CTTGACATACAACCGCTCCT+AGG | 0.517699 | 1.2:-28021088 | None:intergenic |
| GATTCAAAGGGTTCTAAGGA+TGG | 0.534140 | 1.2:+28020956 | MS.gene53537:CDS |
| TAATCAACTTGCTGAGGTGT+TGG | 0.547564 | 1.2:+28021159 | MS.gene53537:CDS |
| AAAATAATAAATAATTACCT+TGG | 0.551962 | 1.2:-28021190 | None:intergenic |
| GTGCAGTGGAATGATAAGAA+TGG | 0.571058 | 1.2:+28021136 | MS.gene53537:CDS |
| ATATTGTTGATGGATTCAAA+GGG | 0.573735 | 1.2:+28020944 | None:intergenic |
| CACAATGGATACACCTGTGG+TGG | 0.583495 | 1.2:+28020988 | MS.gene53537:CDS |
| AAAGTTGAAGATCATGTCAA+TGG | 0.586470 | 1.2:+28021031 | MS.gene53537:CDS |
| TCCATAACACAAACCACCAC+AGG | 0.589813 | 1.2:-28021001 | None:intergenic |
| TCTTTGTTGCCTCCTAGGAG+CGG | 0.611874 | 1.2:+28021076 | MS.gene53537:CDS |
| ATTCAAAGGGTTCTAAGGAT+GGG | 0.614550 | 1.2:+28020957 | MS.gene53537:CDS |
| GAATGGTAATCAACTTGCTG+AGG | 0.618349 | 1.2:+28021153 | MS.gene53537:CDS |
| CAAAGGGTTCTAAGGATGGG+AGG | 0.621872 | 1.2:+28020960 | MS.gene53537:CDS |
| GACATACAACCGCTCCTAGG+AGG | 0.634246 | 1.2:-28021085 | None:intergenic |
| AGACAAGAAGGAAAGTGCAG+TGG | 0.661305 | 1.2:+28021122 | MS.gene53537:CDS |
| TTACACAATGGATACACCTG+TGG | 0.698465 | 1.2:+28020985 | MS.gene53537:CDS |
| GGATGGGAGGAATTACACAA+TGG | 0.703105 | 1.2:+28020973 | MS.gene53537:CDS |
| AGGTGTTGGAATATGAACCA+AGG | 0.714223 | 1.2:+28021173 | MS.gene53537:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGTTGAAGATCATGTCAA+TGG | + | chr1.2:28021031-28021050 | MS.gene53537:CDS | 30.0% | |
| GAAATTCTGGTAAGACAAGA+AGG | + | chr1.2:28021110-28021129 | MS.gene53537:CDS | 35.0% | |
| GGTTGTATGTCAAGAAATTC+TGG | + | chr1.2:28021097-28021116 | MS.gene53537:CDS | 35.0% | |
| !! | ATTCAAAGGGTTCTAAGGAT+GGG | + | chr1.2:28020957-28020976 | MS.gene53537:CDS | 35.0% |
| CTAATTCTTTGTTGCCTCCT+AGG | + | chr1.2:28021071-28021090 | MS.gene53537:CDS | 40.0% | |
| GTGCAGTGGAATGATAAGAA+TGG | + | chr1.2:28021136-28021155 | MS.gene53537:CDS | 40.0% | |
| TTACACAATGGATACACCTG+TGG | + | chr1.2:28020985-28021004 | MS.gene53537:CDS | 40.0% | |
| !! | AGGTGTTGGAATATGAACCA+AGG | + | chr1.2:28021173-28021192 | MS.gene53537:CDS | 40.0% |
| !! | GAATGGTAATCAACTTGCTG+AGG | + | chr1.2:28021153-28021172 | MS.gene53537:CDS | 40.0% |
| !! | GATTCAAAGGGTTCTAAGGA+TGG | + | chr1.2:28020956-28020975 | MS.gene53537:CDS | 40.0% |
| !! | TAATCAACTTGCTGAGGTGT+TGG | + | chr1.2:28021159-28021178 | MS.gene53537:CDS | 40.0% |
| GGATGGGAGGAATTACACAA+TGG | + | chr1.2:28020973-28020992 | MS.gene53537:CDS | 45.0% | |
| TCCATAACACAAACCACCAC+AGG | - | chr1.2:28021004-28021023 | None:intergenic | 45.0% | |
| ! | ACCTGTGGTGGTTTGTGTTA+TGG | + | chr1.2:28021000-28021019 | MS.gene53537:CDS | 45.0% |
| ! | AGACAAGAAGGAAAGTGCAG+TGG | + | chr1.2:28021122-28021141 | MS.gene53537:CDS | 45.0% |
| CACAATGGATACACCTGTGG+TGG | + | chr1.2:28020988-28021007 | MS.gene53537:CDS | 50.0% | |
| CTTGACATACAACCGCTCCT+AGG | - | chr1.2:28021091-28021110 | None:intergenic | 50.0% | |
| TCTTTGTTGCCTCCTAGGAG+CGG | + | chr1.2:28021076-28021095 | MS.gene53537:CDS | 50.0% | |
| !! | CAAAGGGTTCTAAGGATGGG+AGG | + | chr1.2:28020960-28020979 | MS.gene53537:CDS | 50.0% |
| GACATACAACCGCTCCTAGG+AGG | - | chr1.2:28021088-28021107 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 28020953 | 28021198 | 28020953 | ID=MS.gene53537 |
| chr1.2 | mRNA | 28020953 | 28021198 | 28020953 | ID=MS.gene53537.t1;Parent=MS.gene53537 |
| chr1.2 | exon | 28020953 | 28021198 | 28020953 | ID=MS.gene53537.t1.exon1;Parent=MS.gene53537.t1 |
| chr1.2 | CDS | 28020953 | 28021198 | 28020953 | ID=cds.MS.gene53537.t1;Parent=MS.gene53537.t1 |
| Gene Sequence |
| Protein sequence |
