Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062165.t1 | XP_013468859.1 | 99 | 192 | 2 | 0 | 1 | 192 | 276 | 467 | 6.50E-53 | 217.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062165.t1 | Q9M0N1 | 78.4 | 190 | 41 | 0 | 1 | 190 | 290 | 479 | 8.6e-38 | 158.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062165.t1 | A0A072VMS3 | 99.0 | 192 | 2 | 0 | 1 | 192 | 276 | 467 | 4.7e-53 | 217.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene062165 | 0.819117 | 1.37E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene062165 | 0.809967 | 1.46E-50 | -1.69E-46 |
| MS.gene049452 | MS.gene062165 | 0.81389 | 2.04E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene062165 | 0.814476 | 1.51E-51 | -1.69E-46 |
| MS.gene050826 | MS.gene062165 | 0.801227 | 9.94E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene062165 | 0.811274 | 7.60E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene062165 | 0.836549 | 8.77E-57 | -1.69E-46 |
| MS.gene051597 | MS.gene062165 | 0.803932 | 2.75E-49 | -1.69E-46 |
| MS.gene05191 | MS.gene062165 | 0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene062165 | 0.805864 | 1.09E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene062165 | 0.81173 | 6.05E-51 | -1.69E-46 |
| MS.gene053154 | MS.gene062165 | 0.813244 | 2.82E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene062165 | 0.821814 | 3.31E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene062165 | 0.845678 | 3.50E-59 | -1.69E-46 |
| MS.gene054405 | MS.gene062165 | 0.807642 | 4.58E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene062165 | 0.817598 | 3.04E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene062165 | 0.818641 | 1.76E-52 | -1.69E-46 |
| MS.gene054775 | MS.gene062165 | 0.800004 | 1.77E-48 | -1.69E-46 |
| MS.gene055228 | MS.gene062165 | 0.800145 | 1.65E-48 | -1.69E-46 |
| MS.gene055926 | MS.gene062165 | 0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene062165 | 0.815688 | 8.13E-52 | -1.69E-46 |
| MS.gene056272 | MS.gene062165 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene062165 | 0.808593 | 2.87E-50 | -1.69E-46 |
| MS.gene056779 | MS.gene062165 | 0.803568 | 3.28E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene062165 | 0.800505 | 1.40E-48 | -1.69E-46 |
| MS.gene057208 | MS.gene062165 | 0.830697 | 2.54E-55 | -1.69E-46 |
| MS.gene057444 | MS.gene062165 | 0.845916 | 3.01E-59 | -1.69E-46 |
| MS.gene058223 | MS.gene062165 | -0.827866 | 1.23E-54 | -1.69E-46 |
| MS.gene058279 | MS.gene062165 | 0.803497 | 3.39E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene062165 | 0.818559 | 1.84E-52 | -1.69E-46 |
| MS.gene059621 | MS.gene062165 | 0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene062165 | 0.802188 | 6.32E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene062165 | 0.813919 | 2.01E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062165.t1 | MTR_1g077740 | 99.479 | 192 | 1 | 0 | 1 | 192 | 276 | 467 | 1.75e-129 | 372 |
| MS.gene062165.t1 | MTR_4g105430 | 38.462 | 195 | 102 | 4 | 5 | 189 | 184 | 370 | 1.01e-34 | 125 |
| MS.gene062165.t1 | MTR_8g432670 | 30.625 | 160 | 96 | 3 | 32 | 190 | 354 | 499 | 2.12e-14 | 70.9 |
| MS.gene062165.t1 | MTR_4g102810 | 36.667 | 180 | 91 | 5 | 13 | 190 | 421 | 579 | 6.46e-14 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062165.t1 | AT4G10790 | 74.737 | 190 | 48 | 0 | 1 | 190 | 290 | 479 | 2.79e-83 | 254 |
| MS.gene062165.t1 | AT4G23040 | 34.225 | 187 | 104 | 4 | 6 | 190 | 355 | 524 | 2.70e-15 | 73.6 |
| MS.gene062165.t1 | AT4G11740 | 32.222 | 180 | 110 | 3 | 13 | 190 | 394 | 563 | 3.37e-13 | 67.4 |
Find 42 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTCCCTCGGGTTGTTTA+TGG | 0.181565 | 1.2:-62931335 | MS.gene062165:CDS |
| AGCCTTGCTGCAACAAGAAC+TGG | 0.308918 | 1.2:+62932141 | None:intergenic |
| CTTGGTGAAGAACCTGAAAA+AGG | 0.335458 | 1.2:-62931680 | MS.gene062165:CDS |
| CTTTCTTTGCGCACACCATT+GGG | 0.343968 | 1.2:+62931449 | None:intergenic |
| CCTTTCTTTGCGCACACCAT+TGG | 0.365652 | 1.2:+62931448 | None:intergenic |
| GTATGACTATGTTGATTCAT+TGG | 0.383014 | 1.2:-62931393 | MS.gene062165:CDS |
| TACTTTACCTGTGTAACATT+AGG | 0.391398 | 1.2:+62931656 | None:intergenic |
| TATGACTATGTTGATTCATT+GGG | 0.400057 | 1.2:-62931392 | MS.gene062165:CDS |
| GCCAGTCTGTTTGTGGAGCT+CGG | 0.410845 | 1.2:-62931263 | MS.gene062165:CDS |
| ACGAACTAATGCACGCTTAA+GGG | 0.410986 | 1.2:-62932103 | MS.gene062165:CDS |
| AAGAGCAAGATGCTGCATAT+AGG | 0.414768 | 1.2:-62932080 | MS.gene062165:CDS |
| TCCTCAGGCCAGTCTGTTTG+TGG | 0.421845 | 1.2:-62931270 | MS.gene062165:CDS |
| AGAGCAAGATGCTGCATATA+GGG | 0.429045 | 1.2:-62932079 | MS.gene062165:CDS |
| GACGAACTAATGCACGCTTA+AGG | 0.429809 | 1.2:-62932104 | MS.gene062165:CDS |
| GCCTAGTCTCTAACTTCCCT+CGG | 0.451100 | 1.2:-62931346 | MS.gene062165:CDS |
| GGCTCGTGAAAGAGCAGCGC+AGG | 0.457421 | 1.2:-62931759 | MS.gene062165:CDS |
| GCCTTGCTGCAACAAGAACT+GGG | 0.476016 | 1.2:+62932142 | None:intergenic |
| CCCCAGTTCTTGTTGCAGCA+AGG | 0.480156 | 1.2:-62932143 | MS.gene062165:CDS |
| CCAATGGTGTGCGCAAAGAA+AGG | 0.500885 | 1.2:-62931448 | MS.gene062165:CDS |
| CAAAATTGTAGGTGTTGGTA+CGG | 0.502967 | 1.2:-62931475 | MS.gene062165:intron |
| GAAGAGAAAGCTCAATCTCT+TGG | 0.505332 | 1.2:-62931698 | MS.gene062165:CDS |
| AAAATTGGCACTCTCACTAA+AGG | 0.514236 | 1.2:-62931306 | MS.gene062165:CDS |
| GCACTCTCACTAAAGGAAGC+TGG | 0.528422 | 1.2:-62931299 | MS.gene062165:CDS |
| AAGAGGAAGAACGTCTTGCA+AGG | 0.535723 | 1.2:-62931823 | MS.gene062165:CDS |
| GGAAGCTGGATTGCATCCTC+AGG | 0.540277 | 1.2:-62931285 | MS.gene062165:CDS |
| CCTAGTCTCTAACTTCCCTC+GGG | 0.569165 | 1.2:-62931345 | MS.gene062165:CDS |
| ACCGAGCTCCACAAACAGAC+TGG | 0.577057 | 1.2:+62931262 | None:intergenic |
| TGAAGCTGAGAGAAAGCTCA+AGG | 0.590929 | 1.2:-62931792 | MS.gene062165:CDS |
| GAGAAAGCTCAAGGAAGAAG+AGG | 0.595361 | 1.2:-62931783 | MS.gene062165:CDS |
| TTTGTGGAGCTCGGTTCATG+AGG | 0.596829 | 1.2:-62931254 | None:intergenic |
| GTGTTGGTACGGTTTCCCAA+TGG | 0.600493 | 1.2:-62931464 | MS.gene062165:CDS |
| CCCGAGGGAAGTTAGAGACT+AGG | 0.610507 | 1.2:+62931345 | None:intergenic |
| AGAGGAAGAACGTCTTGCAA+GGG | 0.610541 | 1.2:-62931822 | MS.gene062165:CDS |
| GGAACGGCAGAGAAGAGAAG+AGG | 0.621468 | 1.2:-62931840 | MS.gene062165:CDS |
| AAAGCTCAAGGAAGAAGAGG+AGG | 0.627771 | 1.2:-62931780 | MS.gene062165:CDS |
| TATATGTTCAGGCTAGGGAA+CGG | 0.630989 | 1.2:-62931856 | MS.gene062165:intron |
| AAAAGGACCTAATGTTACAC+AGG | 0.641329 | 1.2:-62931663 | MS.gene062165:intron |
| TCTTGTCCATAAACAACCCG+AGG | 0.649081 | 1.2:+62931329 | None:intergenic |
| ATGGTGTGCGCAAAGAAAGG+AGG | 0.657288 | 1.2:-62931445 | MS.gene062165:CDS |
| TCCACAAACAGACTGGCCTG+AGG | 0.673911 | 1.2:+62931269 | None:intergenic |
| CTTGTCCATAAACAACCCGA+GGG | 0.695556 | 1.2:+62931330 | None:intergenic |
| CCTTGCTGCAACAAGAACTG+GGG | 0.706366 | 1.2:+62932143 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATGAGAAACTAAAAA+GGG | + | chr1.2:62931874-62931893 | None:intergenic | 15.0% |
| !!! | TTTTTAGTTTCTCATTTTTT+CGG | - | chr1.2:62931874-62931893 | MS.gene062165:intron | 15.0% |
| !!! | TTTTTTTTTTGTAGTGTATA+TGG | - | chr1.2:62931424-62931443 | MS.gene062165:CDS | 15.0% |
| !! | GAAAAAATGAGAAACTAAAA+AGG | + | chr1.2:62931875-62931894 | None:intergenic | 20.0% |
| !! | TGTTTCATTATGTATTAACT+TGG | - | chr1.2:62931906-62931925 | MS.gene062165:intron | 20.0% |
| !!! | AATTGTATATCATTTTTCTG+TGG | - | chr1.2:62931449-62931468 | MS.gene062165:CDS | 20.0% |
| ! | TGTTTATGGACAAGAAAAAT+TGG | - | chr1.2:62932115-62932134 | MS.gene062165:CDS | 25.0% |
| !! | TCATTATGTATTAACTTGGA+TGG | - | chr1.2:62931910-62931929 | MS.gene062165:intron | 25.0% |
| !!! | TATGACTATGTTGATTCATT+GGG | - | chr1.2:62932044-62932063 | MS.gene062165:intron | 25.0% |
| AGGTTAAGATCTCACATAAA+AGG | + | chr1.2:62931825-62931844 | None:intergenic | 30.0% | |
| GGTTAAGATCTCACATAAAA+GGG | + | chr1.2:62931824-62931843 | None:intergenic | 30.0% | |
| TACTTTACCTGTGTAACATT+AGG | + | chr1.2:62931783-62931802 | None:intergenic | 30.0% | |
| TCTTAACCTCAATTCATATG+TGG | - | chr1.2:62931836-62931855 | MS.gene062165:CDS | 30.0% | |
| TTAATGCTGTGTATATGTTC+AGG | - | chr1.2:62931569-62931588 | MS.gene062165:intron | 30.0% | |
| !! | GTATGACTATGTTGATTCAT+TGG | - | chr1.2:62932043-62932062 | MS.gene062165:intron | 30.0% |
| AAAAGGACCTAATGTTACAC+AGG | - | chr1.2:62931773-62931792 | MS.gene062165:CDS | 35.0% | |
| AAAATTGGCACTCTCACTAA+AGG | - | chr1.2:62932130-62932149 | MS.gene062165:CDS | 35.0% | |
| ACAGGGAAAAACACAAATCA+TGG | + | chr1.2:62931483-62931502 | None:intergenic | 35.0% | |
| AGAAACTAAAAAGGGAGTGA+AGG | + | chr1.2:62931866-62931885 | None:intergenic | 35.0% | |
| CCAAATAAAAGCCTAAGCAA+GGG | + | chr1.2:62931518-62931537 | None:intergenic | 35.0% | |
| CCAATCAAAATTGTAGGTGT+TGG | - | chr1.2:62931956-62931975 | MS.gene062165:intron | 35.0% | |
| CCACTTCCAATCAAAATTGT+AGG | - | chr1.2:62931950-62931969 | MS.gene062165:intron | 35.0% | |
| GGTAAACCACATATGAATTG+AGG | + | chr1.2:62931845-62931864 | None:intergenic | 35.0% | |
| ! | GTAACATTAGGTCCTTTTTC+AGG | + | chr1.2:62931771-62931790 | None:intergenic | 35.0% |
| !! | CAAAATTGTAGGTGTTGGTA+CGG | - | chr1.2:62931961-62931980 | MS.gene062165:intron | 35.0% |
| !! | CCAACACCTACAATTTTGAT+TGG | + | chr1.2:62931959-62931978 | None:intergenic | 35.0% |
| !! | CCTTGCTTAGGCTTTTATTT+GGG | - | chr1.2:62931516-62931535 | MS.gene062165:intron | 35.0% |
| !! | GGCTTTTATTTGGGAAATCT+TGG | - | chr1.2:62931525-62931544 | MS.gene062165:intron | 35.0% |
| !!! | CCTACAATTTTGATTGGAAG+TGG | + | chr1.2:62931953-62931972 | None:intergenic | 35.0% |
| AAGAGCAAGATGCTGCATAT+AGG | - | chr1.2:62931356-62931375 | MS.gene062165:CDS | 40.0% | |
| ACGAACTAATGCACGCTTAA+GGG | - | chr1.2:62931333-62931352 | MS.gene062165:CDS | 40.0% | |
| AGAGCAAGATGCTGCATATA+GGG | - | chr1.2:62931357-62931376 | MS.gene062165:CDS | 40.0% | |
| CCCAAATAAAAGCCTAAGCA+AGG | + | chr1.2:62931519-62931538 | None:intergenic | 40.0% | |
| CTGTGTATATGTTCAGGCTA+GGG | - | chr1.2:62931575-62931594 | MS.gene062165:intron | 40.0% | |
| CTTGGTGAAGAACCTGAAAA+AGG | - | chr1.2:62931756-62931775 | MS.gene062165:CDS | 40.0% | |
| GAAGAGAAAGCTCAATCTCT+TGG | - | chr1.2:62931738-62931757 | MS.gene062165:CDS | 40.0% | |
| TATATGTTCAGGCTAGGGAA+CGG | - | chr1.2:62931580-62931599 | MS.gene062165:intron | 40.0% | |
| ! | AAGGGAACAAGTTGAGAACA+GGG | + | chr1.2:62931500-62931519 | None:intergenic | 40.0% |
| ! | CCCTTGCTTAGGCTTTTATT+TGG | - | chr1.2:62931515-62931534 | MS.gene062165:intron | 40.0% |
| AAAGCTCAAGGAAGAAGAGG+AGG | - | chr1.2:62931656-62931675 | MS.gene062165:intron | 45.0% | |
| AAGAGGAAGAACGTCTTGCA+AGG | - | chr1.2:62931613-62931632 | MS.gene062165:intron | 45.0% | |
| AGAGGAAGAACGTCTTGCAA+GGG | - | chr1.2:62931614-62931633 | MS.gene062165:intron | 45.0% | |
| CTTGTCCATAAACAACCCGA+GGG | + | chr1.2:62932109-62932128 | None:intergenic | 45.0% | |
| CTTTCTTTGCGCACACCATT+GGG | + | chr1.2:62931990-62932009 | None:intergenic | 45.0% | |
| GACGAACTAATGCACGCTTA+AGG | - | chr1.2:62931332-62931351 | MS.gene062165:CDS | 45.0% | |
| GAGAAAGCTCAAGGAAGAAG+AGG | - | chr1.2:62931653-62931672 | MS.gene062165:intron | 45.0% | |
| GCTGTGTATATGTTCAGGCT+AGG | - | chr1.2:62931574-62931593 | MS.gene062165:intron | 45.0% | |
| TCTTGTCCATAAACAACCCG+AGG | + | chr1.2:62932110-62932129 | None:intergenic | 45.0% | |
| TGAAGCTGAGAGAAAGCTCA+AGG | - | chr1.2:62931644-62931663 | MS.gene062165:intron | 45.0% | |
| ! | AACTTCCCTCGGGTTGTTTA+TGG | - | chr1.2:62932101-62932120 | MS.gene062165:CDS | 45.0% |
| ! | CAAGGGAACAAGTTGAGAAC+AGG | + | chr1.2:62931501-62931520 | None:intergenic | 45.0% |
| ! | CTCAACTTGTTCCCTTGCTT+AGG | - | chr1.2:62931504-62931523 | MS.gene062165:intron | 45.0% |
| AGCCTTGCTGCAACAAGAAC+TGG | + | chr1.2:62931298-62931317 | None:intergenic | 50.0% | |
| ATGGTGTGCGCAAAGAAAGG+AGG | - | chr1.2:62931991-62932010 | MS.gene062165:intron | 50.0% | |
| CCAATGGTGTGCGCAAAGAA+AGG | - | chr1.2:62931988-62932007 | MS.gene062165:intron | 50.0% | |
| CCTAGTCTCTAACTTCCCTC+GGG | - | chr1.2:62932091-62932110 | MS.gene062165:CDS | 50.0% | |
| CCTTGCTGCAACAAGAACTG+GGG | + | chr1.2:62931296-62931315 | None:intergenic | 50.0% | |
| CCTTTCTTTGCGCACACCAT+TGG | + | chr1.2:62931991-62932010 | None:intergenic | 50.0% | |
| GCACTCTCACTAAAGGAAGC+TGG | - | chr1.2:62932137-62932156 | MS.gene062165:CDS | 50.0% | |
| GCCTAGTCTCTAACTTCCCT+CGG | - | chr1.2:62932090-62932109 | MS.gene062165:CDS | 50.0% | |
| GCCTTGCTGCAACAAGAACT+GGG | + | chr1.2:62931297-62931316 | None:intergenic | 50.0% | |
| ! | GTGTTGGTACGGTTTCCCAA+TGG | - | chr1.2:62931972-62931991 | MS.gene062165:intron | 50.0% |
| ACCGAGCTCCACAAACAGAC+TGG | + | chr1.2:62932177-62932196 | None:intergenic | 55.0% | |
| CCCCAGTTCTTGTTGCAGCA+AGG | - | chr1.2:62931293-62931312 | MS.gene062165:CDS | 55.0% | |
| CCCGAGGGAAGTTAGAGACT+AGG | + | chr1.2:62932094-62932113 | None:intergenic | 55.0% | |
| GCCAGTCTGTTTGTGGAGCT+CGG | - | chr1.2:62932173-62932192 | MS.gene062165:CDS | 55.0% | |
| GGAACGGCAGAGAAGAGAAG+AGG | - | chr1.2:62931596-62931615 | MS.gene062165:intron | 55.0% | |
| GGAAGCTGGATTGCATCCTC+AGG | - | chr1.2:62932151-62932170 | MS.gene062165:CDS | 55.0% | |
| TCCTCAGGCCAGTCTGTTTG+TGG | - | chr1.2:62932166-62932185 | MS.gene062165:CDS | 55.0% | |
| !! | TCCACAAACAGACTGGCCTG+AGG | + | chr1.2:62932170-62932189 | None:intergenic | 55.0% |
| GGCTCGTGAAAGAGCAGCGC+AGG | - | chr1.2:62931677-62931696 | MS.gene062165:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 62931256 | 62932202 | 62931256 | ID=MS.gene062165 |
| chr1.2 | mRNA | 62931256 | 62932202 | 62931256 | ID=MS.gene062165.t1;Parent=MS.gene062165 |
| chr1.2 | exon | 62932059 | 62932202 | 62932059 | ID=MS.gene062165.t1.exon1;Parent=MS.gene062165.t1 |
| chr1.2 | CDS | 62932059 | 62932202 | 62932059 | ID=cds.MS.gene062165.t1;Parent=MS.gene062165.t1 |
| chr1.2 | exon | 62931664 | 62931867 | 62931664 | ID=MS.gene062165.t1.exon2;Parent=MS.gene062165.t1 |
| chr1.2 | CDS | 62931664 | 62931867 | 62931664 | ID=cds.MS.gene062165.t1;Parent=MS.gene062165.t1 |
| chr1.2 | exon | 62931256 | 62931486 | 62931256 | ID=MS.gene062165.t1.exon3;Parent=MS.gene062165.t1 |
| chr1.2 | CDS | 62931256 | 62931486 | 62931256 | ID=cds.MS.gene062165.t1;Parent=MS.gene062165.t1 |
| Gene Sequence |
| Protein sequence |
