Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06581.t1 | XP_003602417.1 | 99.5 | 184 | 1 | 0 | 1 | 184 | 10 | 193 | 9.10E-97 | 362.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06581.t1 | P30707 | 96.7 | 184 | 6 | 0 | 1 | 184 | 10 | 193 | 6.0e-97 | 354.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06581.t1 | G7J9M6 | 99.5 | 184 | 1 | 0 | 1 | 184 | 10 | 193 | 6.6e-97 | 362.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene06581 | 0.874961 | 4.48E-68 | -1.69E-46 |
| MS.gene050290 | MS.gene06581 | 0.802234 | 6.18E-49 | -1.69E-46 |
| MS.gene050791 | MS.gene06581 | 0.919359 | 4.97E-87 | -1.69E-46 |
| MS.gene050912 | MS.gene06581 | 0.863434 | 2.50E-64 | -1.69E-46 |
| MS.gene051984 | MS.gene06581 | 0.824354 | 8.45E-54 | -1.69E-46 |
| MS.gene053224 | MS.gene06581 | 0.843466 | 1.38E-58 | -1.69E-46 |
| MS.gene056917 | MS.gene06581 | 0.849777 | 2.60E-60 | -1.69E-46 |
| MS.gene057092 | MS.gene06581 | 0.815165 | 1.06E-51 | -1.69E-46 |
| MS.gene057272 | MS.gene06581 | 0.917006 | 8.98E-86 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene06581 | MS.gene43711 | PPI |
| MS.gene06581 | MS.gene057272 | PPI |
| MS.gene06581 | MS.gene06583 | PPI |
| MS.gene06581 | MS.gene87743 | PPI |
| MS.gene43711 | MS.gene06581 | PPI |
| MS.gene06581 | MS.gene42075 | PPI |
| MS.gene06581 | MS.gene82237 | PPI |
| MS.gene06581 | MS.gene77045 | PPI |
| MS.gene06581 | MS.gene48819 | PPI |
| MS.gene06581 | MS.gene013477 | PPI |
| MS.gene06581 | MS.gene06803 | PPI |
| MS.gene06581 | MS.gene87064 | PPI |
| MS.gene06581 | MS.gene76728 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06581.t1 | MTR_3g093110 | 99.457 | 184 | 1 | 0 | 1 | 184 | 10 | 193 | 3.50e-132 | 368 |
| MS.gene06581.t1 | MTR_5g068780 | 86.885 | 183 | 24 | 0 | 1 | 183 | 10 | 192 | 5.68e-120 | 337 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06581.t1 | AT1G33140 | 86.486 | 185 | 24 | 1 | 1 | 184 | 10 | 194 | 2.94e-115 | 325 |
| MS.gene06581.t1 | AT1G33120 | 86.486 | 185 | 24 | 1 | 1 | 184 | 10 | 194 | 2.94e-115 | 325 |
| MS.gene06581.t1 | AT4G10450 | 83.784 | 185 | 29 | 1 | 1 | 184 | 10 | 194 | 1.63e-113 | 321 |
| MS.gene06581.t1 | AT4G10450 | 83.439 | 157 | 25 | 1 | 1 | 156 | 10 | 166 | 1.18e-94 | 273 |
Find 49 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAGATCCGAAACTTTCT+TGG | 0.238218 | 3.2:+78282893 | MS.gene06581:CDS |
| GGTGAAGGATGAGGTTGTTT+TGG | 0.319250 | 3.2:+78283363 | MS.gene06581:CDS |
| ATGACATTGAGCTTGTTTCT+AGG | 0.326351 | 3.2:+78283392 | MS.gene06581:CDS |
| TCGATGCCTGGTTCGGTTCC+AGG | 0.334604 | 3.2:+78282729 | MS.gene06581:CDS |
| TCTTCTCTCCAAGAAAGTTT+CGG | 0.365793 | 3.2:-78282901 | None:intergenic |
| ACCTTGATGCTAACGCCGTC+AGG | 0.384134 | 3.2:-78282590 | None:intergenic |
| CTCAATCGACTTGTTGTCAT+TGG | 0.397158 | 3.2:-78282876 | None:intergenic |
| GGTATTTATGTTAGTGAGAA+GGG | 0.433044 | 3.2:+78283574 | MS.gene06581:CDS |
| CTTCTTCTTGCCTTCTTCAT+CGG | 0.433762 | 3.2:-78282699 | None:intergenic |
| ATTGGTGATGCTAGCGTTGA+TGG | 0.438976 | 3.2:-78282858 | None:intergenic |
| ATGTTAAGAAGAAGGATATC+AGG | 0.444332 | 3.2:+78283539 | MS.gene06581:CDS |
| CTCAAGATCGATGCCTGGTT+CGG | 0.447724 | 3.2:+78282722 | MS.gene06581:CDS |
| GATATCAGGAAGTTTCTTGA+TGG | 0.450730 | 3.2:+78283553 | MS.gene06581:CDS |
| AGGAAAGTAGATTTGCTTGA+TGG | 0.454716 | 3.2:+78283316 | MS.gene06581:CDS |
| AGCAGTTCTGATGGCGGCGG+AGG | 0.457127 | 3.2:-78282756 | None:intergenic |
| AACGCCGTCAGGGATGTTCA+TGG | 0.489928 | 3.2:-78282579 | None:intergenic |
| AAACTTTCTTGGAGAGAAGA+AGG | 0.493598 | 3.2:+78282904 | MS.gene06581:CDS |
| GAAATGCCATGTTAAGAAGA+AGG | 0.499506 | 3.2:+78283531 | MS.gene06581:intron |
| CCCTGACGGCGTTAGCATCA+AGG | 0.507605 | 3.2:+78282589 | MS.gene06581:CDS |
| GATCTCATAACCGATGAAGA+AGG | 0.511263 | 3.2:+78282689 | MS.gene06581:CDS |
| TTCAATTGTTCGATCTGAGA+AGG | 0.526562 | 3.2:+78283342 | MS.gene06581:CDS |
| TGGTATTTATGTTAGTGAGA+AGG | 0.531892 | 3.2:+78283573 | MS.gene06581:CDS |
| TGATATCCTTCTTCTTAACA+TGG | 0.532081 | 3.2:-78283537 | None:intergenic |
| TTGGTGATGCTAGCGTTGAT+GGG | 0.543746 | 3.2:-78282857 | None:intergenic |
| TTATGTTAGTGAGAAGGGAT+CGG | 0.552761 | 3.2:+78283579 | MS.gene06581:CDS |
| CACGTTGAGAATCTCATCAC+TGG | 0.554104 | 3.2:+78282785 | MS.gene06581:CDS |
| CATCTTGTAGCGGAAACCCT+TGG | 0.556289 | 3.2:-78282813 | None:intergenic |
| AGGGTTTCCGCTACAAGATG+AGG | 0.564650 | 3.2:+78282816 | MS.gene06581:CDS |
| ATCTGAGAAGGTGAAGGATG+AGG | 0.567425 | 3.2:+78283354 | MS.gene06581:CDS |
| GAGAGCAGTTCTGATGGCGG+CGG | 0.574544 | 3.2:-78282759 | None:intergenic |
| CTCATCACTGGTGTCACCAA+GGG | 0.576975 | 3.2:+78282797 | MS.gene06581:CDS |
| GTGGCTGAGAGCAGTTCTGA+TGG | 0.581831 | 3.2:-78282765 | None:intergenic |
| TAGCATCAAGGTTAACGCTA+AGG | 0.586158 | 3.2:+78282601 | MS.gene06581:CDS |
| AGAAGCTCAAGATCGATGCC+TGG | 0.588777 | 3.2:+78282717 | MS.gene06581:CDS |
| CCTTGATGCTAACGCCGTCA+GGG | 0.590861 | 3.2:-78282589 | None:intergenic |
| TCGAAGTCGAAGGACCTCGC+GGG | 0.613305 | 3.2:+78282627 | MS.gene06581:CDS |
| TCTCATCACTGGTGTCACCA+AGG | 0.623987 | 3.2:+78282796 | MS.gene06581:CDS |
| GTCATGTGCACTTATTAACC+AGG | 0.625933 | 3.2:+78283414 | MS.gene06581:CDS |
| TGTTCGATCTGAGAAGGTGA+AGG | 0.628133 | 3.2:+78283348 | MS.gene06581:CDS |
| GCTAAGGTCATCGAAGTCGA+AGG | 0.640211 | 3.2:+78282617 | MS.gene06581:CDS |
| TGTTAGTGAGAAGGGATCGG+TGG | 0.651106 | 3.2:+78283582 | MS.gene06581:CDS |
| ATCGAAGTCGAAGGACCTCG+CGG | 0.660265 | 3.2:+78282626 | MS.gene06581:CDS |
| TGGTGATGCTAGCGTTGATG+GGG | 0.676063 | 3.2:-78282856 | None:intergenic |
| GCTGAGAGCAGTTCTGATGG+CGG | 0.677632 | 3.2:-78282762 | None:intergenic |
| CGGTTATGAGATCAAAATCG+AGG | 0.679345 | 3.2:-78282679 | None:intergenic |
| AGACAAACCTCATCTTGTAG+CGG | 0.682924 | 3.2:-78282823 | None:intergenic |
| GAAACCATGAACATCCCTGA+CGG | 0.701649 | 3.2:+78282575 | None:intergenic |
| CAGTGATGAGATTCTCAACG+TGG | 0.725921 | 3.2:-78282784 | None:intergenic |
| TCACGAACAAGCTTCCCGCG+AGG | 0.748381 | 3.2:-78282641 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAATTAGATTAAAACTA+GGG | - | chr3.2:78283041-78283060 | None:intergenic | 10.0% |
| !! | TTATAAATTAGATTAAAACT+AGG | - | chr3.2:78283042-78283061 | None:intergenic | 10.0% |
| !!! | ATGAGTTAATTTTTTTATTT+TGG | + | chr3.2:78283184-78283203 | MS.gene06581:intron | 10.0% |
| !!! | TCTTTAATTTCTTCAATTTT+AGG | + | chr3.2:78282958-78282977 | MS.gene06581:intron | 15.0% |
| !! | GAGATAATGTCTAATTTATT+TGG | + | chr3.2:78283218-78283237 | MS.gene06581:intron | 20.0% |
| ! | ACAAAAATAAAGCATGTTCA+TGG | - | chr3.2:78283458-78283477 | None:intergenic | 25.0% |
| ! | CACAAATTAAAGTGAAACTA+AGG | - | chr3.2:78282992-78283011 | None:intergenic | 25.0% |
| ! | GAATCGAATAATGTTGTATT+AGG | + | chr3.2:78283291-78283310 | MS.gene06581:intron | 25.0% |
| ! | GTCTAATTTATTTGGTTATC+TGG | + | chr3.2:78283226-78283245 | MS.gene06581:intron | 25.0% |
| ATGTTAAGAAGAAGGATATC+AGG | + | chr3.2:78283539-78283558 | MS.gene06581:CDS | 30.0% | |
| GAATAATGTTGTATTAGGTG+AGG | + | chr3.2:78283296-78283315 | MS.gene06581:intron | 30.0% | |
| GGTATTTATGTTAGTGAGAA+GGG | + | chr3.2:78283574-78283593 | MS.gene06581:CDS | 30.0% | |
| TGATATCCTTCTTCTTAACA+TGG | - | chr3.2:78283540-78283559 | None:intergenic | 30.0% | |
| TGGTATTTATGTTAGTGAGA+AGG | + | chr3.2:78283573-78283592 | MS.gene06581:CDS | 30.0% | |
| ! | TTTACTAGTTTGTGAATCTG+TGG | + | chr3.2:78283264-78283283 | MS.gene06581:intron | 30.0% |
| AAACTTTCTTGGAGAGAAGA+AGG | + | chr3.2:78282904-78282923 | MS.gene06581:CDS | 35.0% | |
| ATGTTCATGGACCATGTTAA+GGG | - | chr3.2:78283445-78283464 | None:intergenic | 35.0% | |
| ATTGAGATCCGAAACTTTCT+TGG | + | chr3.2:78282893-78282912 | MS.gene06581:CDS | 35.0% | |
| GAAATGCCATGTTAAGAAGA+AGG | + | chr3.2:78283531-78283550 | MS.gene06581:intron | 35.0% | |
| GATATCAGGAAGTTTCTTGA+TGG | + | chr3.2:78283553-78283572 | MS.gene06581:CDS | 35.0% | |
| TCTTCTCTCCAAGAAAGTTT+CGG | - | chr3.2:78282904-78282923 | None:intergenic | 35.0% | |
| TTATGTTAGTGAGAAGGGAT+CGG | + | chr3.2:78283579-78283598 | MS.gene06581:CDS | 35.0% | |
| TTCAATTGTTCGATCTGAGA+AGG | + | chr3.2:78283342-78283361 | MS.gene06581:CDS | 35.0% | |
| ! | AGGAAAGTAGATTTGCTTGA+TGG | + | chr3.2:78283316-78283335 | MS.gene06581:CDS | 35.0% |
| ! | ATGACATTGAGCTTGTTTCT+AGG | + | chr3.2:78283392-78283411 | MS.gene06581:CDS | 35.0% |
| !! | GAATGTTGTTGTTGCGTTTT+TGG | + | chr3.2:78283504-78283523 | MS.gene06581:intron | 35.0% |
| AGACAAACCTCATCTTGTAG+CGG | - | chr3.2:78282826-78282845 | None:intergenic | 40.0% | |
| CATGTTCATGGACCATGTTA+AGG | - | chr3.2:78283446-78283465 | None:intergenic | 40.0% | |
| CGGTTATGAGATCAAAATCG+AGG | - | chr3.2:78282682-78282701 | None:intergenic | 40.0% | |
| CTTCTTCTTGCCTTCTTCAT+CGG | - | chr3.2:78282702-78282721 | None:intergenic | 40.0% | |
| GATCTCATAACCGATGAAGA+AGG | + | chr3.2:78282689-78282708 | MS.gene06581:CDS | 40.0% | |
| GTCATGTGCACTTATTAACC+AGG | + | chr3.2:78283414-78283433 | MS.gene06581:CDS | 40.0% | |
| TAGCATCAAGGTTAACGCTA+AGG | + | chr3.2:78282601-78282620 | MS.gene06581:CDS | 40.0% | |
| TGTTCATGGACCATGTTAAG+GGG | - | chr3.2:78283444-78283463 | None:intergenic | 40.0% | |
| !! | CTCAATCGACTTGTTGTCAT+TGG | - | chr3.2:78282879-78282898 | None:intergenic | 40.0% |
| !!! | AAGGATGAGGTTGTTTTGGA+TGG | + | chr3.2:78283367-78283386 | MS.gene06581:CDS | 40.0% |
| ATCTGAGAAGGTGAAGGATG+AGG | + | chr3.2:78283354-78283373 | MS.gene06581:CDS | 45.0% | |
| CACGTTGAGAATCTCATCAC+TGG | + | chr3.2:78282785-78282804 | MS.gene06581:CDS | 45.0% | |
| CAGTGATGAGATTCTCAACG+TGG | - | chr3.2:78282787-78282806 | None:intergenic | 45.0% | |
| TGTTCGATCTGAGAAGGTGA+AGG | + | chr3.2:78283348-78283367 | MS.gene06581:CDS | 45.0% | |
| ! | GGTGAAGGATGAGGTTGTTT+TGG | + | chr3.2:78283363-78283382 | MS.gene06581:CDS | 45.0% |
| !! | ATTGGTGATGCTAGCGTTGA+TGG | - | chr3.2:78282861-78282880 | None:intergenic | 45.0% |
| !! | TTGGTGATGCTAGCGTTGAT+GGG | - | chr3.2:78282860-78282879 | None:intergenic | 45.0% |
| ACCAGGTACACCCCTTAACA+TGG | + | chr3.2:78283431-78283450 | MS.gene06581:intron | 50.0% | |
| AGAAGCTCAAGATCGATGCC+TGG | + | chr3.2:78282717-78282736 | MS.gene06581:CDS | 50.0% | |
| AGGGTTTCCGCTACAAGATG+AGG | + | chr3.2:78282816-78282835 | MS.gene06581:CDS | 50.0% | |
| CTCAAGATCGATGCCTGGTT+CGG | + | chr3.2:78282722-78282741 | MS.gene06581:CDS | 50.0% | |
| GCTAAGGTCATCGAAGTCGA+AGG | + | chr3.2:78282617-78282636 | MS.gene06581:CDS | 50.0% | |
| TGTTAGTGAGAAGGGATCGG+TGG | + | chr3.2:78283582-78283601 | MS.gene06581:CDS | 50.0% | |
| ! | ACCATGTTAAGGGGTGTACC+TGG | - | chr3.2:78283435-78283454 | None:intergenic | 50.0% |
| ! | CATCTTGTAGCGGAAACCCT+TGG | - | chr3.2:78282816-78282835 | None:intergenic | 50.0% |
| !! | CTCATCACTGGTGTCACCAA+GGG | + | chr3.2:78282797-78282816 | MS.gene06581:CDS | 50.0% |
| !! | TCTCATCACTGGTGTCACCA+AGG | + | chr3.2:78282796-78282815 | MS.gene06581:CDS | 50.0% |
| !! | TGGTGATGCTAGCGTTGATG+GGG | - | chr3.2:78282859-78282878 | None:intergenic | 50.0% |
| ACCTTGATGCTAACGCCGTC+AGG | - | chr3.2:78282593-78282612 | None:intergenic | 55.0% | |
| ATCGAAGTCGAAGGACCTCG+CGG | + | chr3.2:78282626-78282645 | MS.gene06581:CDS | 55.0% | |
| CCTTGATGCTAACGCCGTCA+GGG | - | chr3.2:78282592-78282611 | None:intergenic | 55.0% | |
| ! | GCTGAGAGCAGTTCTGATGG+CGG | - | chr3.2:78282765-78282784 | None:intergenic | 55.0% |
| ! | GGTTTTCCTGGAACCGAACC+AGG | - | chr3.2:78282738-78282757 | None:intergenic | 55.0% |
| ! | GTGGCTGAGAGCAGTTCTGA+TGG | - | chr3.2:78282768-78282787 | None:intergenic | 55.0% |
| CCCTGACGGCGTTAGCATCA+AGG | + | chr3.2:78282589-78282608 | MS.gene06581:CDS | 60.0% | |
| TCACGAACAAGCTTCCCGCG+AGG | - | chr3.2:78282644-78282663 | None:intergenic | 60.0% | |
| TCGAAGTCGAAGGACCTCGC+GGG | + | chr3.2:78282627-78282646 | MS.gene06581:CDS | 60.0% | |
| !! | GAGAGCAGTTCTGATGGCGG+CGG | - | chr3.2:78282762-78282781 | None:intergenic | 60.0% |
| !! | TCGATGCCTGGTTCGGTTCC+AGG | + | chr3.2:78282729-78282748 | MS.gene06581:CDS | 60.0% |
| !! | AGCAGTTCTGATGGCGGCGG+AGG | - | chr3.2:78282759-78282778 | None:intergenic | 65.0% |
| !! | GATGGCGGCGGAGGTTTTCC+TGG | - | chr3.2:78282750-78282769 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 78282581 | 78283618 | 78282581 | ID=MS.gene06581 |
| chr3.2 | mRNA | 78282581 | 78283618 | 78282581 | ID=MS.gene06581.t1;Parent=MS.gene06581 |
| chr3.2 | exon | 78282581 | 78282925 | 78282581 | ID=MS.gene06581.t1.exon1;Parent=MS.gene06581.t1 |
| chr3.2 | CDS | 78282581 | 78282925 | 78282581 | ID=cds.MS.gene06581.t1;Parent=MS.gene06581.t1 |
| chr3.2 | exon | 78283313 | 78283435 | 78283313 | ID=MS.gene06581.t1.exon2;Parent=MS.gene06581.t1 |
| chr3.2 | CDS | 78283313 | 78283435 | 78283313 | ID=cds.MS.gene06581.t1;Parent=MS.gene06581.t1 |
| chr3.2 | exon | 78283532 | 78283618 | 78283532 | ID=MS.gene06581.t1.exon3;Parent=MS.gene06581.t1 |
| chr3.2 | CDS | 78283532 | 78283618 | 78283532 | ID=cds.MS.gene06581.t1;Parent=MS.gene06581.t1 |
| Gene Sequence |
| Protein sequence |
