Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06583.t1 | XP_003602417.1 | 99.5 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 3.80E-101 | 377.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06583.t1 | P30707 | 96.9 | 193 | 6 | 0 | 1 | 193 | 1 | 193 | 2.5e-101 | 369.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06583.t1 | G7J9M6 | 99.5 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 2.7e-101 | 377.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene06583 | 0.812365 | 4.40E-51 | -1.69E-46 |
| MS.gene050134 | MS.gene06583 | 0.887363 | 1.52E-72 | -1.69E-46 |
| MS.gene050791 | MS.gene06583 | 0.908712 | 1.27E-81 | -1.69E-46 |
| MS.gene050912 | MS.gene06583 | 0.868306 | 7.20E-66 | -1.69E-46 |
| MS.gene051984 | MS.gene06583 | 0.812575 | 3.96E-51 | -1.69E-46 |
| MS.gene052664 | MS.gene06583 | 0.840521 | 8.27E-58 | -1.69E-46 |
| MS.gene053224 | MS.gene06583 | 0.857626 | 1.44E-62 | -1.69E-46 |
| MS.gene056257 | MS.gene06583 | 0.805073 | 1.59E-49 | -1.69E-46 |
| MS.gene056917 | MS.gene06583 | 0.836359 | 9.80E-57 | -1.69E-46 |
| MS.gene057272 | MS.gene06583 | 0.889941 | 1.54E-73 | -1.69E-46 |
| MS.gene057641 | MS.gene06583 | 0.825181 | 5.39E-54 | -1.69E-46 |
| MS.gene057643 | MS.gene06583 | 0.82783 | 1.26E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene06583 | MS.gene057272 | PPI |
| MS.gene48819 | MS.gene06583 | PPI |
| MS.gene06803 | MS.gene06583 | PPI |
| MS.gene013477 | MS.gene06583 | PPI |
| MS.gene77045 | MS.gene06583 | PPI |
| MS.gene06581 | MS.gene06583 | PPI |
| MS.gene42075 | MS.gene06583 | PPI |
| MS.gene87743 | MS.gene06583 | PPI |
| MS.gene87064 | MS.gene06583 | PPI |
| MS.gene06583 | MS.gene013477 | PPI |
| MS.gene057272 | MS.gene06583 | PPI |
| MS.gene43711 | MS.gene06583 | PPI |
| MS.gene76728 | MS.gene06583 | PPI |
| MS.gene82237 | MS.gene06583 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06583.t1 | MTR_3g093110 | 99.482 | 193 | 1 | 0 | 1 | 193 | 1 | 193 | 7.34e-139 | 385 |
| MS.gene06583.t1 | MTR_5g068780 | 87.500 | 192 | 24 | 0 | 1 | 192 | 1 | 192 | 1.36e-126 | 354 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06583.t1 | AT1G33140 | 87.113 | 194 | 24 | 1 | 1 | 193 | 1 | 194 | 9.38e-122 | 342 |
| MS.gene06583.t1 | AT1G33120 | 87.113 | 194 | 24 | 1 | 1 | 193 | 1 | 194 | 9.38e-122 | 342 |
| MS.gene06583.t1 | AT4G10450 | 84.536 | 194 | 29 | 1 | 1 | 193 | 1 | 194 | 2.52e-120 | 338 |
| MS.gene06583.t1 | AT4G10450 | 84.337 | 166 | 25 | 1 | 1 | 165 | 1 | 166 | 2.38e-101 | 290 |
Find 53 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAGATCCGAAACTTTCT+TGG | 0.238218 | 3.3:+77680959 | MS.gene06583:CDS |
| GGTGAAGGATGAGGTTGTTT+TGG | 0.319250 | 3.3:+77681429 | MS.gene06583:CDS |
| ATGACATTGAGCTTGTTTCT+AGG | 0.326351 | 3.3:+77681458 | MS.gene06583:CDS |
| TCGATGCCTGGTTCGGTTCC+AGG | 0.334604 | 3.3:+77680795 | MS.gene06583:CDS |
| GTCAGGGATGTTCATGGTTT+CGG | 0.351741 | 3.3:-77680639 | None:intergenic |
| TCTTCTCTCCAAGAAAGTTT+CGG | 0.365793 | 3.3:-77680967 | None:intergenic |
| ACCTTGATGCTAACGCCGTC+AGG | 0.384134 | 3.3:-77680656 | None:intergenic |
| CTCAATCGACTTGTTGTCAT+TGG | 0.397158 | 3.3:-77680942 | None:intergenic |
| GGTATTTATGTTAGTGAGAA+GGG | 0.433044 | 3.3:+77681640 | MS.gene06583:CDS |
| CTTCTTCTTGCCTTCTTCAT+CGG | 0.433762 | 3.3:-77680765 | None:intergenic |
| ATTGGTGATGCTAGCGTTGA+TGG | 0.438976 | 3.3:-77680924 | None:intergenic |
| ATGTTAAGAAGAAGGATATC+AGG | 0.444332 | 3.3:+77681605 | MS.gene06583:CDS |
| CTCAAGATCGATGCCTGGTT+CGG | 0.447724 | 3.3:+77680788 | MS.gene06583:CDS |
| GATATCAGGAAGTTTCTTGA+TGG | 0.450730 | 3.3:+77681619 | MS.gene06583:CDS |
| AGGAAAGTAGATTTGCTTGA+TGG | 0.454716 | 3.3:+77681382 | MS.gene06583:CDS |
| AGCAGTTCTGATGGCGGCGG+AGG | 0.457127 | 3.3:-77680822 | None:intergenic |
| AACGCCGTCAGGGATGTTCA+TGG | 0.489928 | 3.3:-77680645 | None:intergenic |
| AAACTTTCTTGGAGAGAAGA+AGG | 0.493598 | 3.3:+77680970 | MS.gene06583:CDS |
| GAAATGCCATGTTAAGAAGA+AGG | 0.499506 | 3.3:+77681597 | MS.gene06583:intron |
| CCCTGACGGCGTTAGCATCA+AGG | 0.507605 | 3.3:+77680655 | MS.gene06583:CDS |
| GATCTCATAACCGATGAAGA+AGG | 0.511263 | 3.3:+77680755 | MS.gene06583:CDS |
| TTCAATTGTTCGATCTGAGA+AGG | 0.526562 | 3.3:+77681408 | MS.gene06583:CDS |
| TGGTATTTATGTTAGTGAGA+AGG | 0.531892 | 3.3:+77681639 | MS.gene06583:CDS |
| TGATATCCTTCTTCTTAACA+TGG | 0.532081 | 3.3:-77681603 | None:intergenic |
| CATGGTTTCGGAGGAGAGGA+TGG | 0.534778 | 3.3:-77680627 | None:intergenic |
| TTGGTGATGCTAGCGTTGAT+GGG | 0.543746 | 3.3:-77680923 | None:intergenic |
| TTATGTTAGTGAGAAGGGAT+CGG | 0.552761 | 3.3:+77681645 | MS.gene06583:CDS |
| CACGTTGAGAATCTCATCAC+TGG | 0.554104 | 3.3:+77680851 | MS.gene06583:CDS |
| CATCTTGTAGCGGAAACCCT+TGG | 0.556289 | 3.3:-77680879 | None:intergenic |
| AGGGTTTCCGCTACAAGATG+AGG | 0.564650 | 3.3:+77680882 | MS.gene06583:CDS |
| ATCTGAGAAGGTGAAGGATG+AGG | 0.567425 | 3.3:+77681420 | MS.gene06583:CDS |
| GAGAGCAGTTCTGATGGCGG+CGG | 0.574544 | 3.3:-77680825 | None:intergenic |
| CTCATCACTGGTGTCACCAA+GGG | 0.576975 | 3.3:+77680863 | MS.gene06583:CDS |
| GTGGCTGAGAGCAGTTCTGA+TGG | 0.581831 | 3.3:-77680831 | None:intergenic |
| TGTTCATGGTTTCGGAGGAG+AGG | 0.584113 | 3.3:-77680631 | None:intergenic |
| TAGCATCAAGGTTAACGCTA+AGG | 0.586158 | 3.3:+77680667 | MS.gene06583:CDS |
| AGAAGCTCAAGATCGATGCC+TGG | 0.588777 | 3.3:+77680783 | MS.gene06583:CDS |
| CCTTGATGCTAACGCCGTCA+GGG | 0.590861 | 3.3:-77680655 | None:intergenic |
| TCGAAGTCGAAGGACCTCGC+GGG | 0.613305 | 3.3:+77680693 | MS.gene06583:CDS |
| AGGGATGTTCATGGTTTCGG+AGG | 0.621327 | 3.3:-77680636 | None:intergenic |
| TCTCATCACTGGTGTCACCA+AGG | 0.623987 | 3.3:+77680862 | MS.gene06583:CDS |
| GTCATGTGCACTTATTAACC+AGG | 0.625933 | 3.3:+77681480 | MS.gene06583:CDS |
| TGTTCGATCTGAGAAGGTGA+AGG | 0.628133 | 3.3:+77681414 | MS.gene06583:CDS |
| GCTAAGGTCATCGAAGTCGA+AGG | 0.640211 | 3.3:+77680683 | MS.gene06583:CDS |
| TGTTAGTGAGAAGGGATCGG+TGG | 0.651106 | 3.3:+77681648 | MS.gene06583:CDS |
| ATCGAAGTCGAAGGACCTCG+CGG | 0.660265 | 3.3:+77680692 | MS.gene06583:CDS |
| TGGTGATGCTAGCGTTGATG+GGG | 0.676063 | 3.3:-77680922 | None:intergenic |
| GCTGAGAGCAGTTCTGATGG+CGG | 0.677632 | 3.3:-77680828 | None:intergenic |
| CGGTTATGAGATCAAAATCG+AGG | 0.679345 | 3.3:-77680745 | None:intergenic |
| AGACAAACCTCATCTTGTAG+CGG | 0.682924 | 3.3:-77680889 | None:intergenic |
| GAAACCATGAACATCCCTGA+CGG | 0.701649 | 3.3:+77680641 | MS.gene06583:CDS |
| CAGTGATGAGATTCTCAACG+TGG | 0.725921 | 3.3:-77680850 | None:intergenic |
| TCACGAACAAGCTTCCCGCG+AGG | 0.748381 | 3.3:-77680707 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAATTAGATTAAAACTA+GGG | - | chr3.3:77681107-77681126 | None:intergenic | 10.0% |
| !! | TTATAAATTAGATTAAAACT+AGG | - | chr3.3:77681108-77681127 | None:intergenic | 10.0% |
| !!! | ATGAGTTAATTTTTTTATTT+TGG | + | chr3.3:77681250-77681269 | MS.gene06583:intron | 10.0% |
| !!! | TCTTTAATTTCTTCAATTTT+AGG | + | chr3.3:77681024-77681043 | MS.gene06583:intron | 15.0% |
| !! | GAGATAATGTCTAATTTATT+TGG | + | chr3.3:77681284-77681303 | MS.gene06583:intron | 20.0% |
| ! | ACAAAAATAAAGCATGTTCA+TGG | - | chr3.3:77681524-77681543 | None:intergenic | 25.0% |
| ! | CACAAATTAAAGTGAAACTA+AGG | - | chr3.3:77681058-77681077 | None:intergenic | 25.0% |
| ! | GAATCGAATAATGTTGTATT+AGG | + | chr3.3:77681357-77681376 | MS.gene06583:intron | 25.0% |
| ! | GTCTAATTTATTTGGTTATC+TGG | + | chr3.3:77681292-77681311 | MS.gene06583:intron | 25.0% |
| ATGTTAAGAAGAAGGATATC+AGG | + | chr3.3:77681605-77681624 | MS.gene06583:CDS | 30.0% | |
| GAATAATGTTGTATTAGGTG+AGG | + | chr3.3:77681362-77681381 | MS.gene06583:intron | 30.0% | |
| GGTATTTATGTTAGTGAGAA+GGG | + | chr3.3:77681640-77681659 | MS.gene06583:CDS | 30.0% | |
| TGATATCCTTCTTCTTAACA+TGG | - | chr3.3:77681606-77681625 | None:intergenic | 30.0% | |
| TGGTATTTATGTTAGTGAGA+AGG | + | chr3.3:77681639-77681658 | MS.gene06583:CDS | 30.0% | |
| ! | TTTACTAGTTTGTGAATCTG+TGG | + | chr3.3:77681330-77681349 | MS.gene06583:intron | 30.0% |
| AAACTTTCTTGGAGAGAAGA+AGG | + | chr3.3:77680970-77680989 | MS.gene06583:CDS | 35.0% | |
| ATGTTCATGGACCATGTTAA+GGG | - | chr3.3:77681511-77681530 | None:intergenic | 35.0% | |
| ATTGAGATCCGAAACTTTCT+TGG | + | chr3.3:77680959-77680978 | MS.gene06583:CDS | 35.0% | |
| GAAATGCCATGTTAAGAAGA+AGG | + | chr3.3:77681597-77681616 | MS.gene06583:intron | 35.0% | |
| GATATCAGGAAGTTTCTTGA+TGG | + | chr3.3:77681619-77681638 | MS.gene06583:CDS | 35.0% | |
| TCTTCTCTCCAAGAAAGTTT+CGG | - | chr3.3:77680970-77680989 | None:intergenic | 35.0% | |
| TTATGTTAGTGAGAAGGGAT+CGG | + | chr3.3:77681645-77681664 | MS.gene06583:CDS | 35.0% | |
| TTCAATTGTTCGATCTGAGA+AGG | + | chr3.3:77681408-77681427 | MS.gene06583:CDS | 35.0% | |
| ! | AGGAAAGTAGATTTGCTTGA+TGG | + | chr3.3:77681382-77681401 | MS.gene06583:CDS | 35.0% |
| ! | ATGACATTGAGCTTGTTTCT+AGG | + | chr3.3:77681458-77681477 | MS.gene06583:CDS | 35.0% |
| !! | GAATGTTGTTGTTGCGTTTT+TGG | + | chr3.3:77681570-77681589 | MS.gene06583:intron | 35.0% |
| AGACAAACCTCATCTTGTAG+CGG | - | chr3.3:77680892-77680911 | None:intergenic | 40.0% | |
| CATGTTCATGGACCATGTTA+AGG | - | chr3.3:77681512-77681531 | None:intergenic | 40.0% | |
| CGGTTATGAGATCAAAATCG+AGG | - | chr3.3:77680748-77680767 | None:intergenic | 40.0% | |
| CTTCTTCTTGCCTTCTTCAT+CGG | - | chr3.3:77680768-77680787 | None:intergenic | 40.0% | |
| GATCTCATAACCGATGAAGA+AGG | + | chr3.3:77680755-77680774 | MS.gene06583:CDS | 40.0% | |
| GTCATGTGCACTTATTAACC+AGG | + | chr3.3:77681480-77681499 | MS.gene06583:CDS | 40.0% | |
| TAGCATCAAGGTTAACGCTA+AGG | + | chr3.3:77680667-77680686 | MS.gene06583:CDS | 40.0% | |
| TGTTCATGGACCATGTTAAG+GGG | - | chr3.3:77681510-77681529 | None:intergenic | 40.0% | |
| !! | CTCAATCGACTTGTTGTCAT+TGG | - | chr3.3:77680945-77680964 | None:intergenic | 40.0% |
| !!! | AAGGATGAGGTTGTTTTGGA+TGG | + | chr3.3:77681433-77681452 | MS.gene06583:CDS | 40.0% |
| ATCTGAGAAGGTGAAGGATG+AGG | + | chr3.3:77681420-77681439 | MS.gene06583:CDS | 45.0% | |
| CACGTTGAGAATCTCATCAC+TGG | + | chr3.3:77680851-77680870 | MS.gene06583:CDS | 45.0% | |
| CAGTGATGAGATTCTCAACG+TGG | - | chr3.3:77680853-77680872 | None:intergenic | 45.0% | |
| GAAACCATGAACATCCCTGA+CGG | + | chr3.3:77680641-77680660 | MS.gene06583:CDS | 45.0% | |
| GTCAGGGATGTTCATGGTTT+CGG | - | chr3.3:77680642-77680661 | None:intergenic | 45.0% | |
| TGTTCGATCTGAGAAGGTGA+AGG | + | chr3.3:77681414-77681433 | MS.gene06583:CDS | 45.0% | |
| ! | GGTGAAGGATGAGGTTGTTT+TGG | + | chr3.3:77681429-77681448 | MS.gene06583:CDS | 45.0% |
| !! | ATTGGTGATGCTAGCGTTGA+TGG | - | chr3.3:77680927-77680946 | None:intergenic | 45.0% |
| !! | TTGGTGATGCTAGCGTTGAT+GGG | - | chr3.3:77680926-77680945 | None:intergenic | 45.0% |
| ACCAGGTACACCCCTTAACA+TGG | + | chr3.3:77681497-77681516 | MS.gene06583:intron | 50.0% | |
| AGAAGCTCAAGATCGATGCC+TGG | + | chr3.3:77680783-77680802 | MS.gene06583:CDS | 50.0% | |
| AGGGATGTTCATGGTTTCGG+AGG | - | chr3.3:77680639-77680658 | None:intergenic | 50.0% | |
| AGGGTTTCCGCTACAAGATG+AGG | + | chr3.3:77680882-77680901 | MS.gene06583:CDS | 50.0% | |
| CTCAAGATCGATGCCTGGTT+CGG | + | chr3.3:77680788-77680807 | MS.gene06583:CDS | 50.0% | |
| GCTAAGGTCATCGAAGTCGA+AGG | + | chr3.3:77680683-77680702 | MS.gene06583:CDS | 50.0% | |
| TGTTAGTGAGAAGGGATCGG+TGG | + | chr3.3:77681648-77681667 | MS.gene06583:CDS | 50.0% | |
| TGTTCATGGTTTCGGAGGAG+AGG | - | chr3.3:77680634-77680653 | None:intergenic | 50.0% | |
| ! | ACCATGTTAAGGGGTGTACC+TGG | - | chr3.3:77681501-77681520 | None:intergenic | 50.0% |
| ! | CATCTTGTAGCGGAAACCCT+TGG | - | chr3.3:77680882-77680901 | None:intergenic | 50.0% |
| !! | CTCATCACTGGTGTCACCAA+GGG | + | chr3.3:77680863-77680882 | MS.gene06583:CDS | 50.0% |
| !! | TCTCATCACTGGTGTCACCA+AGG | + | chr3.3:77680862-77680881 | MS.gene06583:CDS | 50.0% |
| !! | TGGTGATGCTAGCGTTGATG+GGG | - | chr3.3:77680925-77680944 | None:intergenic | 50.0% |
| AACGCCGTCAGGGATGTTCA+TGG | - | chr3.3:77680648-77680667 | None:intergenic | 55.0% | |
| ACCTTGATGCTAACGCCGTC+AGG | - | chr3.3:77680659-77680678 | None:intergenic | 55.0% | |
| ATCGAAGTCGAAGGACCTCG+CGG | + | chr3.3:77680692-77680711 | MS.gene06583:CDS | 55.0% | |
| CATGGTTTCGGAGGAGAGGA+TGG | - | chr3.3:77680630-77680649 | None:intergenic | 55.0% | |
| CCTTGATGCTAACGCCGTCA+GGG | - | chr3.3:77680658-77680677 | None:intergenic | 55.0% | |
| ! | GCTGAGAGCAGTTCTGATGG+CGG | - | chr3.3:77680831-77680850 | None:intergenic | 55.0% |
| ! | GGTTTTCCTGGAACCGAACC+AGG | - | chr3.3:77680804-77680823 | None:intergenic | 55.0% |
| ! | GTGGCTGAGAGCAGTTCTGA+TGG | - | chr3.3:77680834-77680853 | None:intergenic | 55.0% |
| CCCTGACGGCGTTAGCATCA+AGG | + | chr3.3:77680655-77680674 | MS.gene06583:CDS | 60.0% | |
| TCACGAACAAGCTTCCCGCG+AGG | - | chr3.3:77680710-77680729 | None:intergenic | 60.0% | |
| TCGAAGTCGAAGGACCTCGC+GGG | + | chr3.3:77680693-77680712 | MS.gene06583:CDS | 60.0% | |
| !! | GAGAGCAGTTCTGATGGCGG+CGG | - | chr3.3:77680828-77680847 | None:intergenic | 60.0% |
| !! | TCGATGCCTGGTTCGGTTCC+AGG | + | chr3.3:77680795-77680814 | MS.gene06583:CDS | 60.0% |
| !! | AGCAGTTCTGATGGCGGCGG+AGG | - | chr3.3:77680825-77680844 | None:intergenic | 65.0% |
| !! | GATGGCGGCGGAGGTTTTCC+TGG | - | chr3.3:77680816-77680835 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 77680620 | 77681684 | 77680620 | ID=MS.gene06583 |
| chr3.3 | mRNA | 77680620 | 77681684 | 77680620 | ID=MS.gene06583.t1;Parent=MS.gene06583 |
| chr3.3 | exon | 77680620 | 77680991 | 77680620 | ID=MS.gene06583.t1.exon1;Parent=MS.gene06583.t1 |
| chr3.3 | CDS | 77680620 | 77680991 | 77680620 | ID=cds.MS.gene06583.t1;Parent=MS.gene06583.t1 |
| chr3.3 | exon | 77681379 | 77681501 | 77681379 | ID=MS.gene06583.t1.exon2;Parent=MS.gene06583.t1 |
| chr3.3 | CDS | 77681379 | 77681501 | 77681379 | ID=cds.MS.gene06583.t1;Parent=MS.gene06583.t1 |
| chr3.3 | exon | 77681598 | 77681684 | 77681598 | ID=MS.gene06583.t1.exon3;Parent=MS.gene06583.t1 |
| chr3.3 | CDS | 77681598 | 77681684 | 77681598 | ID=cds.MS.gene06583.t1;Parent=MS.gene06583.t1 |
| Gene Sequence |
| Protein sequence |
