AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06803


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06803.t1 MTR_3g093110 99.457 184 1 0 1 184 10 193 3.50e-132 368
MS.gene06803.t1 MTR_5g068780 86.885 183 24 0 1 183 10 192 5.68e-120 337
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06803.t1 AT1G33140 86.486 185 24 1 1 184 10 194 2.94e-115 325
MS.gene06803.t1 AT1G33120 86.486 185 24 1 1 184 10 194 2.94e-115 325
MS.gene06803.t1 AT4G10450 83.784 185 29 1 1 184 10 194 1.63e-113 321
MS.gene06803.t1 AT4G10450 83.439 157 25 1 1 156 10 166 1.18e-94 273

Find 0 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAATTAGATTAAAACTA+GGG + 24276:8737-8756 MS.gene06803:intergenic 10.0%
!! TTATAAATTAGATTAAAACT+AGG + 24276:8738-8757 MS.gene06803:intergenic 10.0%
!!! ATGAGTTAATTTTTTTATTT+TGG - 24276:8880-8899 MS.gene06803:intron 10.0%
!!! TCTTTAATTTCTTCAATTTT+AGG - 24276:8654-8673 MS.gene06803:intron 15.0%
!! GAGATAATGTCTAATTTATT+TGG - 24276:8914-8933 MS.gene06803:intron 20.0%
! ACAAAAATAAAGCATGTTCA+TGG + 24276:9154-9173 MS.gene06803:intergenic 25.0%
! CACAAATTAAAGTGAAACTA+AGG + 24276:8688-8707 MS.gene06803:intergenic 25.0%
! GAATCGAATAATGTTGTATT+AGG - 24276:8987-9006 MS.gene06803:CDS 25.0%
! GTCTAATTTATTTGGTTATC+TGG - 24276:8922-8941 MS.gene06803:intron 25.0%
ATGTTAAGAAGAAGGATATC+AGG - 24276:9235-9254 MS.gene06803:CDS 30.0%
GAATAATGTTGTATTAGGTG+AGG - 24276:8992-9011 MS.gene06803:CDS 30.0%
GGTATTTATGTTAGTGAGAA+GGG - 24276:9270-9289 MS.gene06803:CDS 30.0%
TGATATCCTTCTTCTTAACA+TGG + 24276:9236-9255 MS.gene06803:intergenic 30.0%
TGGTATTTATGTTAGTGAGA+AGG - 24276:9269-9288 MS.gene06803:CDS 30.0%
! TTTACTAGTTTGTGAATCTG+TGG - 24276:8960-8979 MS.gene06803:intron 30.0%
AAACTTTCTTGGAGAGAAGA+AGG - 24276:8600-8619 MS.gene06803:intron 35.0%
ATGTTCATGGACCATGTTAA+GGG + 24276:9141-9160 MS.gene06803:intergenic 35.0%
ATTGAGATCCGAAACTTTCT+TGG - 24276:8589-8608 MS.gene06803:intron 35.0%
GAAATGCCATGTTAAGAAGA+AGG - 24276:9227-9246 MS.gene06803:CDS 35.0%
GATATCAGGAAGTTTCTTGA+TGG - 24276:9249-9268 MS.gene06803:CDS 35.0%
TCTTCTCTCCAAGAAAGTTT+CGG + 24276:8600-8619 MS.gene06803:intergenic 35.0%
TTATGTTAGTGAGAAGGGAT+CGG - 24276:9275-9294 MS.gene06803:CDS 35.0%
TTCAATTGTTCGATCTGAGA+AGG - 24276:9038-9057 MS.gene06803:CDS 35.0%
! AGGAAAGTAGATTTGCTTGA+TGG - 24276:9012-9031 MS.gene06803:CDS 35.0%
! ATGACATTGAGCTTGTTTCT+AGG - 24276:9088-9107 MS.gene06803:CDS 35.0%
!! GAATGTTGTTGTTGCGTTTT+TGG - 24276:9200-9219 MS.gene06803:CDS 35.0%
AGACAAACCTCATCTTGTAG+CGG + 24276:8522-8541 MS.gene06803:intergenic 40.0%
CATGTTCATGGACCATGTTA+AGG + 24276:9142-9161 MS.gene06803:intergenic 40.0%
CGGTTATGAGATCAAAATCG+AGG + 24276:8378-8397 MS.gene06803:intergenic 40.0%
CTTCTTCTTGCCTTCTTCAT+CGG + 24276:8398-8417 MS.gene06803:intergenic 40.0%
GATCTCATAACCGATGAAGA+AGG - 24276:8385-8404 MS.gene06803:intron 40.0%
GTCATGTGCACTTATTAACC+AGG - 24276:9110-9129 MS.gene06803:CDS 40.0%
TAGCATCAAGGTTAACGCTA+AGG - 24276:8297-8316 MS.gene06803:CDS 40.0%
TGTTCATGGACCATGTTAAG+GGG + 24276:9140-9159 MS.gene06803:intergenic 40.0%
!! CTCAATCGACTTGTTGTCAT+TGG + 24276:8575-8594 MS.gene06803:intergenic 40.0%
!!! AAGGATGAGGTTGTTTTGGA+TGG - 24276:9063-9082 MS.gene06803:CDS 40.0%
ATCTGAGAAGGTGAAGGATG+AGG - 24276:9050-9069 MS.gene06803:CDS 45.0%
CACGTTGAGAATCTCATCAC+TGG - 24276:8481-8500 MS.gene06803:CDS 45.0%
CAGTGATGAGATTCTCAACG+TGG + 24276:8483-8502 MS.gene06803:intergenic 45.0%
TGTTCGATCTGAGAAGGTGA+AGG - 24276:9044-9063 MS.gene06803:CDS 45.0%
! GGTGAAGGATGAGGTTGTTT+TGG - 24276:9059-9078 MS.gene06803:CDS 45.0%
!! ATTGGTGATGCTAGCGTTGA+TGG + 24276:8557-8576 MS.gene06803:intergenic 45.0%
!! TTGGTGATGCTAGCGTTGAT+GGG + 24276:8556-8575 MS.gene06803:intergenic 45.0%
ACCAGGTACACCCCTTAACA+TGG - 24276:9127-9146 MS.gene06803:CDS 50.0%
AGAAGCTCAAGATCGATGCC+TGG - 24276:8413-8432 MS.gene06803:intron 50.0%
AGGGTTTCCGCTACAAGATG+AGG - 24276:8512-8531 MS.gene06803:CDS 50.0%
CTCAAGATCGATGCCTGGTT+CGG - 24276:8418-8437 MS.gene06803:intron 50.0%
GCTAAGGTCATCGAAGTCGA+AGG - 24276:8313-8332 MS.gene06803:CDS 50.0%
TGTTAGTGAGAAGGGATCGG+TGG - 24276:9278-9297 MS.gene06803:CDS 50.0%
! ACCATGTTAAGGGGTGTACC+TGG + 24276:9131-9150 MS.gene06803:intergenic 50.0%
! CATCTTGTAGCGGAAACCCT+TGG + 24276:8512-8531 MS.gene06803:intergenic 50.0%
!! CTCATCACTGGTGTCACCAA+GGG - 24276:8493-8512 MS.gene06803:CDS 50.0%
!! TCTCATCACTGGTGTCACCA+AGG - 24276:8492-8511 MS.gene06803:CDS 50.0%
!! TGGTGATGCTAGCGTTGATG+GGG + 24276:8555-8574 MS.gene06803:intergenic 50.0%
ACCTTGATGCTAACGCCGTC+AGG + 24276:8289-8308 MS.gene06803:intergenic 55.0%
ATCGAAGTCGAAGGACCTCG+CGG - 24276:8322-8341 MS.gene06803:CDS 55.0%
CCTTGATGCTAACGCCGTCA+GGG + 24276:8288-8307 MS.gene06803:intergenic 55.0%
! GCTGAGAGCAGTTCTGATGG+CGG + 24276:8461-8480 MS.gene06803:intergenic 55.0%
! GGTTTTCCTGGAACCGAACC+AGG + 24276:8434-8453 MS.gene06803:intergenic 55.0%
! GTGGCTGAGAGCAGTTCTGA+TGG + 24276:8464-8483 MS.gene06803:intergenic 55.0%
CCCTGACGGCGTTAGCATCA+AGG - 24276:8285-8304 MS.gene06803:CDS 60.0%
TCACGAACAAGCTTCCCGCG+AGG + 24276:8340-8359 MS.gene06803:intergenic 60.0%
TCGAAGTCGAAGGACCTCGC+GGG - 24276:8323-8342 MS.gene06803:CDS 60.0%
!! GAGAGCAGTTCTGATGGCGG+CGG + 24276:8458-8477 MS.gene06803:intergenic 60.0%
!! TCGATGCCTGGTTCGGTTCC+AGG - 24276:8425-8444 MS.gene06803:intron 60.0%
!! AGCAGTTCTGATGGCGGCGG+AGG + 24276:8455-8474 MS.gene06803:intergenic 65.0%
!! GATGGCGGCGGAGGTTTTCC+TGG + 24276:8446-8465 MS.gene06803:intergenic 65.0%


Chromosome Type Strat End Strand Name
24276 gene 8277 9314 8277 ID=MS.gene06803
24276 mRNA 8277 9314 8277 ID=MS.gene06803.t1;Parent=MS.gene06803
24276 exon 8970 9314 8970 ID=MS.gene06803.t1.exon1;Parent=MS.gene06803.t1
24276 CDS 8970 9314 8970 ID=cds.MS.gene06803.t1;Parent=MS.gene06803.t1
24276 exon 8460 8582 8460 ID=MS.gene06803.t1.exon2;Parent=MS.gene06803.t1
24276 CDS 8460 8582 8460 ID=cds.MS.gene06803.t1;Parent=MS.gene06803.t1
24276 exon 8277 8363 8277 ID=MS.gene06803.t1.exon3;Parent=MS.gene06803.t1
24276 CDS 8277 8363 8277 ID=cds.MS.gene06803.t1;Parent=MS.gene06803.t1
Gene Sequence

>MS.gene06803

ATGAACATCCCTGACGGCGTTAGCATCAAGGTTAACGCTAAGGTCATCGAAGTCGAAGGACCTCGCGGGAAGCTTGTTCGTGATTTCAAGCATCTCAACCTCGATTTTGATCTCATAACCGATGAAGAAGGCAAGAAGAAGCTCAAGATCGATGCCTGGTTCGGTTCCAGGAAAACCTCCGCCGCCATCAGAACTGCTCTCAGCCACGTTGAGAATCTCATCACTGGTGTCACCAAGGGTTTCCGCTACAAGATGAGGTTTGTCTATGCTCATTTCCCCATCAACGCTAGCATCACCAATGACAACAAGTCGATTGAGATCCGAAACTTTCTTGGAGAGAAGAAGGTATTCAATTTTTTTCGTAATCATTATTTTTATCTTTAATTTCTTCAATTTTAGGTTAAATAACCTTAGTTTCACTTTAATTTGTGATCTTATGCAATTTTAGACTTGATAACCCTAGTTTTAATCTAATTTATAATCTGTAATTAGATCATTTTGTAATAATTTTCATATATTACTTACGTTAAACATTAATTTTTATTAGTGCTAATGTTTTTTTGTGATTAAAAAACGAGTTAATCTTTTATCTGTTGATTAAAAATGAGTTAATTTTTTTATTTTGGTAATTAAAAATGAGATAATGTCTAATTTATTTGGTTATCTGGTTTAGTTATTTGTGATTTACTAGTTTGTGAATCTGTGGTATTGAATCGAATAATGTTGTATTAGGTGAGGAAAGTAGATTTGCTTGATGGAGTTTCAATTGTTCGATCTGAGAAGGTGAAGGATGAGGTTGTTTTGGATGGAAATGACATTGAGCTTGTTTCTAGGTCATGTGCACTTATTAACCAGGTACACCCCTTAACATGGTCCATGAACATGCTTTATTTTTGTGCATTTTGCTCTTTGTTTATGTTTCTGAATGTTGTTGTTGCGTTTTTGGAACAGAAATGCCATGTTAAGAAGAAGGATATCAGGAAGTTTCTTGATGGTATTTATGTTAGTGAGAAGGGATCGGTGGTAACTGAAGAATAG

Protein sequence

>MS.gene06803.t1

MNIPDGVSIKVNAKVIEVEGPRGKLVRDFKHLNLDFDLITDEEGKKKLKIDAWFGSRKTSAAIRTALSHVENLITGVTKGFRYKMRFVYAHFPINASITNDNKSIEIRNFLGEKKVRKVDLLDGVSIVRSEKVKDEVVLDGNDIELVSRSCALINQKCHVKKKDIRKFLDGIYVSEKGSVVTEE