AlfalfaGEDB | MS.gene065820

M. sativa cultivar XinJiangDaYe / MS.gene065820

KEGG: K07904
GO: GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00071.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065820.t1	XP_013468899.1	98.1	107	2	0	1	107	118	224	2.00E-48	201.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065820.t1	Q9FGK5	76.6	107	20	2	1	106	118	220	2.5e-34	146.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065820.t1	A0A072VMV1	98.1	107	2	0	1	107	118	224	1.5e-48	201.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene065820	0.858313	8.97E-63	-1.69E-46
MS.gene049873	MS.gene065820	0.802258	6.11E-49	-1.69E-46
MS.gene050026	MS.gene065820	0.806754	7.05E-50	-1.69E-46
MS.gene050559	MS.gene065820	0.82365	1.24E-53	-1.69E-46
MS.gene050561	MS.gene065820	0.81325	2.81E-51	-1.69E-46
MS.gene050562	MS.gene065820	0.808869	2.51E-50	-1.69E-46
MS.gene050799	MS.gene065820	0.813741	2.20E-51	-1.69E-46
MS.gene051279	MS.gene065820	0.815238	1.02E-51	-1.69E-46
MS.gene051744	MS.gene065820	0.814162	1.77E-51	-1.69E-46
MS.gene052108	MS.gene065820	0.801943	7.09E-49	-1.69E-46
MS.gene052519	MS.gene065820	0.841546	4.45E-58	-1.69E-46
MS.gene052582	MS.gene065820	0.816607	5.07E-52	-1.69E-46
MS.gene052584	MS.gene065820	0.819963	8.81E-53	-1.69E-46
MS.gene053082	MS.gene065820	0.800374	1.49E-48	-1.69E-46
MS.gene053226	MS.gene065820	0.818074	2.37E-52	-1.69E-46
MS.gene053599	MS.gene065820	0.806783	6.96E-50	-1.69E-46
MS.gene05415	MS.gene065820	0.805305	1.42E-49	-1.69E-46
MS.gene05423	MS.gene065820	0.814111	1.82E-51	-1.69E-46
MS.gene054651	MS.gene065820	0.83877	2.36E-57	-1.69E-46
MS.gene054991	MS.gene065820	0.802116	6.54E-49	-1.69E-46
MS.gene055586	MS.gene065820	0.801602	8.33E-49	-1.69E-46
MS.gene055818	MS.gene065820	0.821574	3.76E-53	-1.69E-46
MS.gene056050	MS.gene065820	0.810685	1.02E-50	-1.69E-46
MS.gene056272	MS.gene065820	0.806861	6.70E-50	-1.69E-46
MS.gene056595	MS.gene065820	0.803799	2.93E-49	-1.69E-46
MS.gene056776	MS.gene065820	0.803821	2.90E-49	-1.69E-46
MS.gene05686	MS.gene065820	0.800129	1.67E-48	-1.69E-46
MS.gene057102	MS.gene065820	0.800894	1.16E-48	-1.69E-46
MS.gene057208	MS.gene065820	0.821854	3.23E-53	-1.69E-46
MS.gene057444	MS.gene065820	0.806455	8.16E-50	-1.69E-46
MS.gene058037	MS.gene065820	0.821581	3.74E-53	-1.69E-46
MS.gene058625	MS.gene065820	0.830439	2.93E-55	-1.69E-46
MS.gene05954	MS.gene065820	0.803226	3.86E-49	-1.69E-46
MS.gene059625	MS.gene065820	0.805197	1.50E-49	-1.69E-46
MS.gene060352	MS.gene065820	0.804077	2.57E-49	-1.69E-46
MS.gene060365	MS.gene065820	0.814997	1.16E-51	-1.69E-46
MS.gene060521	MS.gene065820	0.832888	7.31E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065820.t1	MTR_1g078200	98.131	107	2	0	1	107	118	224	1.60e-72	215
MS.gene065820.t1	MTR_7g405790	49.074	108	43	2	3	106	121	220	3.70e-25	94.4
MS.gene065820.t1	MTR_7g051940	61.644	73	28	0	3	75	119	191	3.83e-24	91.7
MS.gene065820.t1	MTR_4g064897	45.714	105	50	3	2	106	117	214	2.41e-22	87.0
MS.gene065820.t1	MTR_8g089985	57.143	70	30	0	2	71	122	191	2.97e-22	87.0
MS.gene065820.t1	MTR_3g086980	43.269	104	56	1	2	105	121	221	5.03e-22	86.3
MS.gene065820.t1	MTR_4g079350	44.762	105	52	3	2	106	118	216	5.01e-20	80.9
MS.gene065820.t1	MTR_2g014990	43.810	105	53	3	2	106	118	216	1.93e-19	79.7
MS.gene065820.t1	MTR_1g052475	40.952	105	59	3	2	105	120	222	2.00e-19	79.7
MS.gene065820.t1	MTR_2g005510	42.857	105	55	2	2	106	120	219	2.41e-19	79.3
MS.gene065820.t1	MTR_0262s0050	39.423	104	59	1	2	105	120	219	2.84e-19	79.3
MS.gene065820.t1	MTR_4g088090	40.952	105	56	3	2	106	118	216	2.57e-18	76.6
MS.gene065820.t1	MTR_8g036220	39.252	107	63	1	2	106	121	227	2.85e-18	76.6
MS.gene065820.t1	MTR_2g083860	51.515	66	32	0	2	67	118	183	6.99e-18	75.5
MS.gene065820.t1	MTR_2g075950	40.000	105	57	2	2	106	118	216	3.84e-17	73.6
MS.gene065820.t1	MTR_2g090865	40.385	104	55	2	3	106	117	213	4.64e-17	73.2
MS.gene065820.t1	MTR_3g069510	40.952	105	56	3	2	106	117	215	6.83e-17	72.8
MS.gene065820.t1	MTR_6g079550	51.515	66	32	0	2	67	117	182	1.52e-14	66.6
MS.gene065820.t1	MTR_8g027220	50.000	66	33	0	2	67	131	196	2.10e-14	66.6
MS.gene065820.t1	MTR_3g064390	38.095	105	46	2	2	106	115	200	6.25e-13	62.0
MS.gene065820.t1	MTR_5g076590	35.238	105	49	3	2	106	115	200	2.12e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065820.t1	AT5G47520	76.636	107	20	2	1	106	118	220	4.93e-49	155
MS.gene065820.t1	AT3G07410	48.571	105	49	2	3	107	118	217	3.38e-23	89.4
MS.gene065820.t1	AT5G47960	44.340	106	52	2	2	105	120	220	4.03e-22	86.7
MS.gene065820.t1	AT2G43130	43.810	105	51	2	3	107	118	214	1.40e-21	85.1
MS.gene065820.t1	AT3G12160	39.623	106	60	1	2	107	120	221	1.83e-21	85.1
MS.gene065820.t1	AT4G18430	41.905	105	55	2	2	106	118	216	2.66e-21	84.3
MS.gene065820.t1	AT5G65270	52.857	70	33	0	2	71	122	191	3.47e-21	84.3
MS.gene065820.t1	AT2G31680	45.192	104	53	1	3	106	118	217	7.22e-21	83.2
MS.gene065820.t1	AT1G07410	41.905	105	53	3	2	106	117	213	1.28e-20	82.4
MS.gene065820.t1	AT5G59150	43.810	105	54	2	2	106	66	165	2.47e-20	80.9
MS.gene065820.t1	AT4G39990	52.174	69	33	0	2	70	122	190	3.66e-20	81.6
MS.gene065820.t1	AT5G60860	43.810	105	53	2	2	106	118	216	5.65e-20	80.9
MS.gene065820.t1	AT1G05810	57.746	71	30	0	3	73	118	188	6.54e-20	80.9
MS.gene065820.t1	AT5G59150	43.810	105	54	2	2	106	117	216	6.99e-20	80.9
MS.gene065820.t1	AT1G09630	40.952	105	56	2	2	106	117	215	7.37e-20	80.5
MS.gene065820.t1	AT1G05810	57.746	71	30	0	3	73	164	234	1.21e-19	80.9
MS.gene065820.t1	AT1G28550	41.905	105	56	2	2	106	118	217	6.01e-19	78.2
MS.gene065820.t1	AT3G46830	40.952	105	57	1	2	106	117	216	1.24e-18	77.4
MS.gene065820.t1	AT3G46830	40.952	105	57	1	2	106	117	216	1.24e-18	77.4
MS.gene065820.t1	AT1G18200	39.091	110	61	2	4	107	120	229	2.52e-18	77.0
MS.gene065820.t1	AT3G15060	42.453	106	53	3	2	106	118	216	3.58e-18	76.3
MS.gene065820.t1	AT4G18800	40.952	105	53	2	2	106	118	213	5.36e-18	75.9
MS.gene065820.t1	AT1G16920	43.810	105	52	3	2	106	118	215	2.54e-17	73.9
MS.gene065820.t1	AT1G06400	39.048	105	57	2	2	106	118	215	2.14e-16	71.6
MS.gene065820.t1	AT5G45750	39.048	105	57	2	2	106	118	215	2.26e-16	71.6
MS.gene065820.t1	AT2G33870	39.048	105	59	2	2	106	118	217	7.64e-16	70.1
MS.gene065820.t1	AT1G73640	49.206	63	32	0	4	66	120	182	1.09e-15	70.1
MS.gene065820.t1	AT5G46025	51.724	58	28	0	3	60	51	108	6.29e-14	62.8
MS.gene065820.t1	AT1G02130	38.095	105	50	3	2	106	113	202	5.68e-12	59.7
MS.gene065820.t1	AT3G54840	47.368	57	30	0	2	58	105	161	1.48e-11	58.2
MS.gene065820.t1	AT4G19640	36.190	105	48	3	2	106	115	200	1.50e-11	58.5
MS.gene065820.t1	AT3G54840	47.368	57	30	0	2	58	139	195	2.06e-11	58.2
MS.gene065820.t1	AT4G17160	37.500	104	55	3	2	105	111	204	6.60e-11	57.0
MS.gene065820.t1	AT3G54840	50.980	51	25	0	2	52	139	189	9.20e-11	56.2

Find 23 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCACAAGGTTTGTTCTTTA+TGG	0.121880	1.3:-55965501	MS.gene065820:CDS
AAAGGGTTGTTGTTCGAAAT+AGG	0.304511	1.3:-55965288	None:intergenic
GATGCTTCCTGGATTGAAAA+TGG	0.318182	1.3:-55965365	MS.gene065820:CDS
TCAAGAAAGACGATGCTTCC+TGG	0.368001	1.3:-55965376	MS.gene065820:CDS
TGGGAACAAGTCAGATCTTA+AGG	0.381456	1.3:-55965567	MS.gene065820:CDS
CTTGAGACATCATAACCTTC+CGG	0.418307	1.3:+55965402	None:intergenic
AACGTTGTCACAATACTTGT+TGG	0.431302	1.3:-55965587	MS.gene065820:CDS
ACGTTGTCACAATACTTGTT+GGG	0.487177	1.3:-55965586	MS.gene065820:CDS
GAAGGGGATCGATCAGCAGA+AGG	0.552374	1.3:-55965320	MS.gene065820:CDS
ACTGTTGTTCTACAACAAGA+AGG	0.557959	1.3:-55965338	MS.gene065820:CDS
CTGTTGTTCTACAACAAGAA+GGG	0.571816	1.3:-55965337	MS.gene065820:CDS
AGATCTTAAGGATGCGAGAG+AGG	0.573972	1.3:-55965555	MS.gene065820:CDS
AAATCTACAACATATTAAGC+CGG	0.576659	1.3:-55965421	MS.gene065820:CDS
AGGGGATCGATCAGCAGAAG+GGG	0.577182	1.3:-55965318	MS.gene065820:CDS
AAGGGGATCGATCAGCAGAA+GGG	0.592624	1.3:-55965319	MS.gene065820:CDS
GGTACCTACGACGGAAGGCA+AGG	0.605827	1.3:-55965534	MS.gene065820:CDS
AGAGAGGTACCTACGACGGA+AGG	0.610104	1.3:-55965539	MS.gene065820:CDS
TGTTGTTCTACAACAAGAAG+GGG	0.621100	1.3:-55965336	MS.gene065820:CDS
AACGCCTTGCCTTCCGTCGT+AGG	0.621241	1.3:+55965530	None:intergenic
CTACAACATATTAAGCCGGA+AGG	0.622382	1.3:-55965417	MS.gene065820:CDS
AAGGCGTTAGCTGAGGCACA+AGG	0.638041	1.3:-55965515	MS.gene065820:CDS
GGAAGGCAAGGCGTTAGCTG+AGG	0.641771	1.3:-55965522	MS.gene065820:CDS
TGCGAGAGAGGTACCTACGA+CGG	0.743487	1.3:-55965543	MS.gene065820:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATCTACAACATATTAAGC+CGG	-	chr1.3:55965458-55965477	MS.gene065820:CDS	25.0%
	AACGTTGTCACAATACTTGT+TGG	-	chr1.3:55965292-55965311	MS.gene065820:CDS	35.0%
	ACGTTGTCACAATACTTGTT+GGG	-	chr1.3:55965293-55965312	MS.gene065820:CDS	35.0%
	ACTGTTGTTCTACAACAAGA+AGG	-	chr1.3:55965541-55965560	MS.gene065820:CDS	35.0%
	CTGTTGTTCTACAACAAGAA+GGG	-	chr1.3:55965542-55965561	MS.gene065820:CDS	35.0%
	TGTTGTTCTACAACAAGAAG+GGG	-	chr1.3:55965543-55965562	MS.gene065820:CDS	35.0%
!	AGTCTTTCCATTTTCAATCC+AGG	+	chr1.3:55965524-55965543	None:intergenic	35.0%
	CTACAACATATTAAGCCGGA+AGG	-	chr1.3:55965462-55965481	MS.gene065820:CDS	40.0%
	CTTGAGACATCATAACCTTC+CGG	+	chr1.3:55965480-55965499	None:intergenic	40.0%
	GATGCTTCCTGGATTGAAAA+TGG	-	chr1.3:55965514-55965533	MS.gene065820:CDS	40.0%
	TGGGAACAAGTCAGATCTTA+AGG	-	chr1.3:55965312-55965331	MS.gene065820:CDS	40.0%
!	GGCACAAGGTTTGTTCTTTA+TGG	-	chr1.3:55965378-55965397	MS.gene065820:CDS	40.0%
	AGATCTTAAGGATGCGAGAG+AGG	-	chr1.3:55965324-55965343	MS.gene065820:CDS	45.0%
	AGCAGAAGGGGAGACAAAAA+AGG	-	chr1.3:55965573-55965592	MS.gene065820:CDS	45.0%
	GCAGAAGGGGAGACAAAAAA+GGG	-	chr1.3:55965574-55965593	MS.gene065820:CDS	45.0%
	TCAAGAAAGACGATGCTTCC+TGG	-	chr1.3:55965503-55965522	MS.gene065820:CDS	45.0%
	AAGGGGATCGATCAGCAGAA+GGG	-	chr1.3:55965560-55965579	MS.gene065820:CDS	50.0%
	AGGGGATCGATCAGCAGAAG+GGG	-	chr1.3:55965561-55965580	MS.gene065820:CDS	55.0%
	GAAGGGGATCGATCAGCAGA+AGG	-	chr1.3:55965559-55965578	MS.gene065820:CDS	55.0%
!	AAGGCGTTAGCTGAGGCACA+AGG	-	chr1.3:55965364-55965383	MS.gene065820:CDS	55.0%
!!	AGAGAGGTACCTACGACGGA+AGG	-	chr1.3:55965340-55965359	MS.gene065820:CDS	55.0%
!!	TGCGAGAGAGGTACCTACGA+CGG	-	chr1.3:55965336-55965355	MS.gene065820:CDS	55.0%
	AACGCCTTGCCTTCCGTCGT+AGG	+	chr1.3:55965352-55965371	None:intergenic	60.0%
	GGTACCTACGACGGAAGGCA+AGG	-	chr1.3:55965345-55965364	MS.gene065820:CDS	60.0%
!	GGAAGGCAAGGCGTTAGCTG+AGG	-	chr1.3:55965357-55965376	MS.gene065820:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	55965289	55965612	55965289	ID=MS.gene065820
chr1.3	mRNA	55965289	55965612	55965289	ID=MS.gene065820.t1;Parent=MS.gene065820
chr1.3	exon	55965289	55965612	55965289	ID=MS.gene065820.t1.exon1;Parent=MS.gene065820.t1
chr1.3	CDS	55965289	55965612	55965289	ID=cds.MS.gene065820.t1;Parent=MS.gene065820.t1

Gene Sequence	>MS.gene065820 ATGAACGTTGTCACAATACTTGTTGGGAACAAGTCAGATCTTAAGGATGCGAGAGAGGTACCTACGACGGAAGGCAAGGCGTTAGCTGAGGCACAAGGTTTGTTCTTTATGGAGACATCAGCACTTGATTCATCAAATGTAGTTGCTGCTTTTCAAACAGTTGTAAAAGAAATCTACAACATATTAAGCCGGAAGGTTATGATGTCTCAAGAGCTCAAGAAAGACGATGCTTCCTGGATTGAAAATGGAAAGACTGTTGTTCTACAACAAGAAGGGGATCGATCAGCAGAAGGGGAGACAAAAAAGGGTTGTTGTTCGAAATAG
Protein sequence	>MS.gene065820.t1 MNVVTILVGNKSDLKDAREVPTTEGKALAEAQGLFFMETSALDSSNVVAAFQTVVKEIYNILSRKVMMSQELKKDDASWIENGKTVVLQQEGDRSAEGETKKGCCSK