Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065906.t1 | GAU51333.1 | 53.3 | 169 | 77 | 2 | 1 | 169 | 321 | 487 | 3.40E-37 | 164.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065906.t1 | P08770 | 35.3 | 119 | 68 | 4 | 58 | 169 | 611 | 727 | 1.7e-08 | 60.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065906.t1 | A0A2Z6P8S5 | 53.3 | 169 | 77 | 2 | 1 | 169 | 321 | 487 | 2.4e-37 | 164.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049501 | MS.gene065906 | 0.803844 | 2.87E-49 | -1.69E-46 |
| MS.gene05016 | MS.gene065906 | 0.81533 | 9.77E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene065906 | 0.808537 | 2.95E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene065906 | 0.809984 | 1.44E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene065906 | 0.808445 | 3.09E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene065906 | 0.816301 | 5.93E-52 | -1.69E-46 |
| MS.gene050680 | MS.gene065906 | 0.801238 | 9.89E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene065906 | 0.819966 | 8.80E-53 | -1.69E-46 |
| MS.gene050721 | MS.gene065906 | 0.81824 | 2.17E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene065906 | 0.808801 | 2.59E-50 | -1.69E-46 |
| MS.gene050780 | MS.gene065906 | 0.820802 | 5.66E-53 | -1.69E-46 |
| MS.gene050820 | MS.gene065906 | 0.810964 | 8.87E-51 | -1.69E-46 |
| MS.gene050844 | MS.gene065906 | 0.810184 | 1.31E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene065906 | 0.809199 | 2.13E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene065906 | 0.825798 | 3.85E-54 | -1.69E-46 |
| MS.gene052012 | MS.gene065906 | 0.809461 | 1.87E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene065906 | 0.82963 | 4.62E-55 | -1.69E-46 |
| MS.gene052190 | MS.gene065906 | 0.806812 | 6.86E-50 | -1.69E-46 |
| MS.gene052208 | MS.gene065906 | 0.801392 | 9.20E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene065906 | 0.806516 | 7.92E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene065906 | 0.806661 | 7.38E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene065906 | 0.811016 | 8.64E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene065906 | 0.811236 | 7.74E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene065906 | 0.834885 | 2.31E-56 | -1.69E-46 |
| MS.gene056173 | MS.gene065906 | 0.802035 | 6.79E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene065906 | 0.822132 | 2.79E-53 | -1.69E-46 |
| MS.gene056553 | MS.gene065906 | 0.812905 | 3.35E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene065906 | 0.814316 | 1.64E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene065906 | 0.83878 | 2.35E-57 | -1.69E-46 |
| MS.gene058168 | MS.gene065906 | 0.809189 | 2.14E-50 | -1.69E-46 |
| MS.gene058426 | MS.gene065906 | 0.806351 | 8.58E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene065906 | 0.809094 | 2.24E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene065906 | 0.800139 | 1.66E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene065906 | 0.82337 | 1.44E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene065906 | 0.815948 | 7.11E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene065906 | -0.805054 | 1.61E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATGGTTAAAGAAATTT+TGG | 0.148511 | 4.1:+27120594 | MS.gene065906:CDS |
| CGGGATATATAGAGTGGTTT+GGG | 0.285076 | 4.1:+27119908 | MS.gene065906:CDS |
| ACCTCGTTACCTAATCTTCT+TGG | 0.299458 | 4.1:+27120570 | MS.gene065906:CDS |
| CCATTGCAGCCAAGAAGATT+AGG | 0.365219 | 4.1:-27120579 | None:intergenic |
| GACCTTCTAGTTGCACTGAT+TGG | 0.373487 | 4.1:+27119938 | MS.gene065906:CDS |
| TGTTTCCACCAAGCAAGAAT+TGG | 0.383338 | 4.1:-27120541 | None:intergenic |
| AAATTAGAAGGTAAAGATTC+GGG | 0.393470 | 4.1:+27119889 | MS.gene065906:CDS |
| TCGGGATATATAGAGTGGTT+TGG | 0.398167 | 4.1:+27119907 | MS.gene065906:CDS |
| ACCTTCTAGTTGCACTGATT+GGG | 0.411703 | 4.1:+27119939 | MS.gene065906:CDS |
| GGATGCTGCTGAGAAGTTCC+AGG | 0.414997 | 4.1:+27119855 | MS.gene065906:CDS |
| TCCCAATCAGTGCAACTAGA+AGG | 0.415746 | 4.1:-27119940 | None:intergenic |
| AAAATTAGAAGGTAAAGATT+CGG | 0.420972 | 4.1:+27119888 | MS.gene065906:CDS |
| GCTGCTGAGAAGTTCCAGGC+TGG | 0.452751 | 4.1:+27119859 | MS.gene065906:CDS |
| CGACGAAGAGAAAACCTTTG+TGG | 0.454167 | 4.1:-27120007 | None:intergenic |
| ACAAGTTTCCAATTCTTGCT+TGG | 0.486783 | 4.1:+27120533 | MS.gene065906:CDS |
| TCTGATGCGACTGTTAAAAC+TGG | 0.490845 | 4.1:-27120622 | None:intergenic |
| ATAGAGTGGTTTGGGGCTGT+TGG | 0.494682 | 4.1:+27119916 | MS.gene065906:CDS |
| CCTAATCTTCTTGGCTGCAA+TGG | 0.495367 | 4.1:+27120579 | MS.gene065906:CDS |
| GTCGTTATCATTTGCACAAG+GGG | 0.509201 | 4.1:-27120512 | None:intergenic |
| CTGCTGAGAAGTTCCAGGCT+GGG | 0.511223 | 4.1:+27119860 | MS.gene065906:CDS |
| AGTTTCCAATTCTTGCTTGG+TGG | 0.519643 | 4.1:+27120536 | MS.gene065906:CDS |
| TCAGAGAGTGCATTTAGCAC+AGG | 0.531281 | 4.1:+27120640 | MS.gene065906:CDS |
| TTCTCGATGTTTCCACAAGG+TGG | 0.569312 | 4.1:+27119812 | MS.gene065906:CDS |
| GAACTCGACATACATGATGT+TGG | 0.578899 | 4.1:+27119834 | MS.gene065906:CDS |
| AAGATTCGGGATATATAGAG+TGG | 0.584923 | 4.1:+27119902 | MS.gene065906:CDS |
| GTATGTCGAGTTCCACCTTG+TGG | 0.586835 | 4.1:-27119824 | None:intergenic |
| CAGAGAGTGCATTTAGCACA+GGG | 0.586912 | 4.1:+27120641 | MS.gene065906:CDS |
| TTGTCGTTATCATTTGCACA+AGG | 0.605999 | 4.1:-27120514 | None:intergenic |
| GAGAGTGCATTTAGCACAGG+GGG | 0.628909 | 4.1:+27120643 | MS.gene065906:CDS |
| GATTGTCTATGATGCCACAA+AGG | 0.644427 | 4.1:+27119993 | MS.gene065906:CDS |
| ATAAGCATAATGAACTTGAG+AGG | 0.645264 | 4.1:+27120479 | MS.gene065906:CDS |
| TGTCGTTATCATTTGCACAA+GGG | 0.654269 | 4.1:-27120513 | None:intergenic |
| GGGATATATAGAGTGGTTTG+GGG | 0.674165 | 4.1:+27119909 | MS.gene065906:CDS |
| GCCAAGAAGATTAGGTAACG+AGG | 0.679633 | 4.1:-27120571 | None:intergenic |
| TGCGACTGTTAAAACTGGTG+TGG | 0.682984 | 4.1:-27120617 | None:intergenic |
| AGAGAGTGCATTTAGCACAG+GGG | 0.786774 | 4.1:+27120642 | MS.gene065906:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTAGAAGGTAAAGATT+CGG | + | chr4.1:27119888-27119907 | MS.gene065906:CDS | 20.0% |
| !! | ATATATCTGAACTTAAAATG+AGG | - | chr4.1:27120221-27120240 | None:intergenic | 20.0% |
| !! | ATCTGTTTAAATTATACGAT+TGG | + | chr4.1:27120316-27120335 | MS.gene065906:intron | 20.0% |
| !! | ATGACATTTCAAAAGAAATA+TGG | + | chr4.1:27120275-27120294 | MS.gene065906:intron | 20.0% |
| !! | TCTGTTTAAATTATACGATT+GGG | + | chr4.1:27120317-27120336 | MS.gene065906:intron | 20.0% |
| ! | AAATATGGTCAAGAATGTTA+AGG | + | chr4.1:27120290-27120309 | MS.gene065906:intron | 25.0% |
| ! | AAATTAGAAGGTAAAGATTC+GGG | + | chr4.1:27119889-27119908 | MS.gene065906:CDS | 25.0% |
| ! | TTAAGTTCAGATATATTGAG+TGG | + | chr4.1:27120225-27120244 | MS.gene065906:intron | 25.0% |
| !! | TGCAATGGTTAAAGAAATTT+TGG | + | chr4.1:27120594-27120613 | MS.gene065906:CDS | 25.0% |
| ATAAGCATAATGAACTTGAG+AGG | + | chr4.1:27120479-27120498 | MS.gene065906:CDS | 30.0% | |
| GGTTAAGATTGATTGTTTGA+TGG | - | chr4.1:27120391-27120410 | None:intergenic | 30.0% | |
| TAAATTATACGATTGGGTCA+AGG | + | chr4.1:27120323-27120342 | MS.gene065906:intron | 30.0% | |
| !! | GTCACATTCATTTTGATCAT+GGG | - | chr4.1:27120352-27120371 | None:intergenic | 30.0% |
| !!! | AATTCTCTTTTTTGCCTCAA+TGG | + | chr4.1:27120108-27120127 | MS.gene065906:intron | 30.0% |
| !!! | ATTCTCTTTTTTGCCTCAAT+GGG | + | chr4.1:27120109-27120128 | MS.gene065906:intron | 30.0% |
| AAGATTCGGGATATATAGAG+TGG | + | chr4.1:27119902-27119921 | MS.gene065906:CDS | 35.0% | |
| ACAAGTTTCCAATTCTTGCT+TGG | + | chr4.1:27120533-27120552 | MS.gene065906:CDS | 35.0% | |
| AGCGGAAATATGACAAGTAT+TGG | + | chr4.1:27120143-27120162 | MS.gene065906:intron | 35.0% | |
| GCGGAAATATGACAAGTATT+GGG | + | chr4.1:27120144-27120163 | MS.gene065906:intron | 35.0% | |
| TGTCGTTATCATTTGCACAA+GGG | - | chr4.1:27120516-27120535 | None:intergenic | 35.0% | |
| TTGATCCAGTTTATGAGGTT+GGG | + | chr4.1:27120252-27120271 | MS.gene065906:intron | 35.0% | |
| TTGTCGTTATCATTTGCACA+AGG | - | chr4.1:27120517-27120536 | None:intergenic | 35.0% | |
| TTTGATCCAGTTTATGAGGT+TGG | + | chr4.1:27120251-27120270 | MS.gene065906:intron | 35.0% | |
| ! | CGGAAATATGACAAGTATTG+GGG | + | chr4.1:27120145-27120164 | MS.gene065906:intron | 35.0% |
| ! | TTTTTCTCGATGTTTCCACA+AGG | + | chr4.1:27119809-27119828 | MS.gene065906:CDS | 35.0% |
| !! | GGTCACATTCATTTTGATCA+TGG | - | chr4.1:27120353-27120372 | None:intergenic | 35.0% |
| ACCTCGTTACCTAATCTTCT+TGG | + | chr4.1:27120570-27120589 | MS.gene065906:CDS | 40.0% | |
| AGTTTCCAATTCTTGCTTGG+TGG | + | chr4.1:27120536-27120555 | MS.gene065906:CDS | 40.0% | |
| CGGGATATATAGAGTGGTTT+GGG | + | chr4.1:27119908-27119927 | MS.gene065906:CDS | 40.0% | |
| CTTCATATCAACACCCATTG+AGG | - | chr4.1:27120125-27120144 | None:intergenic | 40.0% | |
| GAACTCGACATACATGATGT+TGG | + | chr4.1:27119834-27119853 | MS.gene065906:CDS | 40.0% | |
| GATTGTCTATGATGCCACAA+AGG | + | chr4.1:27119993-27120012 | MS.gene065906:CDS | 40.0% | |
| GCAAAGAGTGAAGGATTTCT+AGG | - | chr4.1:27120412-27120431 | None:intergenic | 40.0% | |
| GCTGGGTGTGAAAAATTAGA+AGG | + | chr4.1:27119877-27119896 | MS.gene065906:CDS | 40.0% | |
| GGGATATATAGAGTGGTTTG+GGG | + | chr4.1:27119909-27119928 | MS.gene065906:CDS | 40.0% | |
| GTCGTTATCATTTGCACAAG+GGG | - | chr4.1:27120515-27120534 | None:intergenic | 40.0% | |
| GTCGTTTGATCCAGTTTATG+AGG | + | chr4.1:27120247-27120266 | MS.gene065906:intron | 40.0% | |
| TCGGGATATATAGAGTGGTT+TGG | + | chr4.1:27119907-27119926 | MS.gene065906:CDS | 40.0% | |
| TCTGATGCGACTGTTAAAAC+TGG | - | chr4.1:27120625-27120644 | None:intergenic | 40.0% | |
| TGTTTCCACCAAGCAAGAAT+TGG | - | chr4.1:27120544-27120563 | None:intergenic | 40.0% | |
| !! | ACCTTCTAGTTGCACTGATT+GGG | + | chr4.1:27119939-27119958 | MS.gene065906:CDS | 40.0% |
| !! | CAATGGGTGTTGATATGAAG+CGG | + | chr4.1:27120125-27120144 | MS.gene065906:intron | 40.0% |
| AATGTGACCTCACAGCTTGT+TGG | + | chr4.1:27120364-27120383 | MS.gene065906:intron | 45.0% | |
| CCATTGCAGCCAAGAAGATT+AGG | - | chr4.1:27120582-27120601 | None:intergenic | 45.0% | |
| CCTAATCTTCTTGGCTGCAA+TGG | + | chr4.1:27120579-27120598 | MS.gene065906:CDS | 45.0% | |
| CGACGAAGAGAAAACCTTTG+TGG | - | chr4.1:27120010-27120029 | None:intergenic | 45.0% | |
| GCCAAGAAGATTAGGTAACG+AGG | - | chr4.1:27120574-27120593 | None:intergenic | 45.0% | |
| GTCATCCCAACCTCATAAAC+TGG | - | chr4.1:27120260-27120279 | None:intergenic | 45.0% | |
| TCAGCTCTTGCAAAGAGTGA+AGG | - | chr4.1:27120421-27120440 | None:intergenic | 45.0% | |
| TCCCAATCAGTGCAACTAGA+AGG | - | chr4.1:27119943-27119962 | None:intergenic | 45.0% | |
| TGCGACTGTTAAAACTGGTG+TGG | - | chr4.1:27120620-27120639 | None:intergenic | 45.0% | |
| TTCTCGATGTTTCCACAAGG+TGG | + | chr4.1:27119812-27119831 | MS.gene065906:CDS | 45.0% | |
| ! | AGAGAGTGCATTTAGCACAG+GGG | + | chr4.1:27120642-27120661 | MS.gene065906:CDS | 45.0% |
| ! | CAGAGAGTGCATTTAGCACA+GGG | + | chr4.1:27120641-27120660 | MS.gene065906:CDS | 45.0% |
| ! | CTAATTTTTCACACCCAGCC+TGG | - | chr4.1:27119876-27119895 | None:intergenic | 45.0% |
| ! | GACCTTCTAGTTGCACTGAT+TGG | + | chr4.1:27119938-27119957 | MS.gene065906:CDS | 45.0% |
| ! | TCAGAGAGTGCATTTAGCAC+AGG | + | chr4.1:27120640-27120659 | MS.gene065906:CDS | 45.0% |
| GTATGTCGAGTTCCACCTTG+TGG | - | chr4.1:27119827-27119846 | None:intergenic | 50.0% | |
| ! | ATAGAGTGGTTTGGGGCTGT+TGG | + | chr4.1:27119916-27119935 | MS.gene065906:CDS | 50.0% |
| ! | GAGAGTGCATTTAGCACAGG+GGG | + | chr4.1:27120643-27120662 | MS.gene065906:CDS | 50.0% |
| ! | TGATGGACCAACAAGCTGTG+AGG | - | chr4.1:27120374-27120393 | None:intergenic | 50.0% |
| CTGCTGAGAAGTTCCAGGCT+GGG | + | chr4.1:27119860-27119879 | MS.gene065906:CDS | 55.0% | |
| GGATGCTGCTGAGAAGTTCC+AGG | + | chr4.1:27119855-27119874 | MS.gene065906:CDS | 55.0% | |
| GCTGCTGAGAAGTTCCAGGC+TGG | + | chr4.1:27119859-27119878 | MS.gene065906:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 27119805 | 27120675 | 27119805 | ID=MS.gene065906 |
| chr4.1 | mRNA | 27119805 | 27120675 | 27119805 | ID=MS.gene065906.t1;Parent=MS.gene065906 |
| chr4.1 | exon | 27119805 | 27120078 | 27119805 | ID=MS.gene065906.t1.exon1;Parent=MS.gene065906.t1 |
| chr4.1 | CDS | 27119805 | 27120078 | 27119805 | ID=cds.MS.gene065906.t1;Parent=MS.gene065906.t1 |
| chr4.1 | exon | 27120437 | 27120675 | 27120437 | ID=MS.gene065906.t1.exon2;Parent=MS.gene065906.t1 |
| chr4.1 | CDS | 27120437 | 27120675 | 27120437 | ID=cds.MS.gene065906.t1;Parent=MS.gene065906.t1 |
| Gene Sequence |
| Protein sequence |
