Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066272.t1 | XP_003592191.1 | 89 | 136 | 14 | 1 | 13 | 147 | 1 | 136 | 3.80E-45 | 191 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066272.t1 | O03982 | 56.6 | 76 | 32 | 1 | 13 | 87 | 1 | 76 | 4.1e-16 | 85.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066272.t1 | G7IDY9 | 89.0 | 136 | 14 | 1 | 13 | 147 | 1 | 136 | 2.7e-45 | 191.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene066272 | 0.877287 | 7.09E-69 | -1.69E-46 |
| MS.gene051676 | MS.gene066272 | 0.802147 | 6.44E-49 | -1.69E-46 |
| MS.gene053094 | MS.gene066272 | 0.801959 | 7.04E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene066272 | 0.833353 | 5.60E-56 | -1.69E-46 |
| MS.gene053340 | MS.gene066272 | 0.811011 | 8.67E-51 | -1.69E-46 |
| MS.gene053879 | MS.gene066272 | 0.803874 | 2.83E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene066272 | 0.853119 | 2.95E-61 | -1.69E-46 |
| MS.gene054222 | MS.gene066272 | 0.812185 | 4.82E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene066272 | 0.867742 | 1.09E-65 | -1.69E-46 |
| MS.gene054893 | MS.gene066272 | 0.872511 | 3.01E-67 | -1.69E-46 |
| MS.gene055147 | MS.gene066272 | 0.802078 | 6.65E-49 | -1.69E-46 |
| MS.gene055148 | MS.gene066272 | 0.80485 | 1.77E-49 | -1.69E-46 |
| MS.gene055150 | MS.gene066272 | 0.807503 | 4.90E-50 | -1.69E-46 |
| MS.gene055226 | MS.gene066272 | 0.804808 | 1.81E-49 | -1.69E-46 |
| MS.gene057093 | MS.gene066272 | 0.814878 | 1.23E-51 | -1.69E-46 |
| MS.gene057155 | MS.gene066272 | 0.80463 | 1.97E-49 | -1.69E-46 |
| MS.gene057622 | MS.gene066272 | 0.851634 | 7.81E-61 | -1.69E-46 |
| MS.gene057999 | MS.gene066272 | 0.801238 | 9.90E-49 | -1.69E-46 |
| MS.gene058526 | MS.gene066272 | 0.811186 | 7.94E-51 | -1.69E-46 |
| MS.gene058527 | MS.gene066272 | 0.81039 | 1.18E-50 | -1.69E-46 |
| MS.gene058528 | MS.gene066272 | 0.81223 | 4.71E-51 | -1.69E-46 |
| MS.gene05878 | MS.gene066272 | 0.811965 | 5.38E-51 | -1.69E-46 |
| MS.gene05952 | MS.gene066272 | 0.833947 | 3.98E-56 | -1.69E-46 |
| MS.gene05956 | MS.gene066272 | 0.802985 | 4.33E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene066272 | -0.812401 | 4.32E-51 | -1.69E-46 |
| MS.gene06023 | MS.gene066272 | 0.827773 | 1.30E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene066272 | 0.834342 | 3.17E-56 | -1.69E-46 |
| MS.gene060412 | MS.gene066272 | 0.82877 | 7.48E-55 | -1.69E-46 |
| MS.gene061047 | MS.gene066272 | 0.857846 | 1.24E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCAGTCTCTGGCCTTTC+AGG | 0.147290 | 1.1:-71779098 | MS.gene066272:intron |
| AGGATCCTCTTCAACGCTTA+TGG | 0.283035 | 1.1:+71778488 | None:intergenic |
| ATTAATCCTTTCGTGCTCTA+TGG | 0.313770 | 1.1:-71778567 | MS.gene066272:CDS |
| TGGATCAATGTCTCCTATTA+AGG | 0.318014 | 1.1:+71778728 | None:intergenic |
| GACAAGAAAATGACCTTAAT+AGG | 0.340701 | 1.1:-71778741 | MS.gene066272:CDS |
| TACTAACCTTCATATTGTTA+TGG | 0.356206 | 1.1:+71779366 | None:intergenic |
| ATTCATCTGATGATTATAAT+AGG | 0.369814 | 1.1:+71778533 | None:intergenic |
| ACGAAAGGATTAATTATCAT+TGG | 0.419156 | 1.1:+71778576 | None:intergenic |
| TAGATGATGACACAGCCATT+AGG | 0.441354 | 1.1:+71778468 | None:intergenic |
| ACAGAAAGTTGTGTTGAAGT+TGG | 0.461760 | 1.1:-71779156 | MS.gene066272:intron |
| TTCAGTTGGACCAGCTAAAG+AGG | 0.475094 | 1.1:-71778658 | MS.gene066272:CDS |
| AAAGCTATGAAAGCAGTCTC+TGG | 0.485853 | 1.1:-71779107 | MS.gene066272:CDS |
| AGCTTAGCCACTACACGTAC+TGG | 0.496210 | 1.1:+71778708 | None:intergenic |
| CATGCTGAAATACTTTCAGT+TGG | 0.503143 | 1.1:-71778672 | MS.gene066272:CDS |
| GCACGGTGGTATGAATTGTA+TGG | 0.520307 | 1.1:+71778510 | None:intergenic |
| TGATGATTATAATAGGTAGT+AGG | 0.521368 | 1.1:+71778540 | None:intergenic |
| CCGTGCCATAAGCGTTGAAG+AGG | 0.546888 | 1.1:-71778493 | MS.gene066272:CDS |
| TAGCTTCCATAACAATATGA+AGG | 0.555459 | 1.1:-71779372 | MS.gene066272:intron |
| TACGTGTAGTGGCTAAGCTA+AGG | 0.568064 | 1.1:-71778704 | MS.gene066272:CDS |
| TAGTTAATTCAATACCTGAA+AGG | 0.578105 | 1.1:+71779084 | None:intergenic |
| AGCGTTGAAGAGGATCCTAA+TGG | 0.594188 | 1.1:-71778483 | MS.gene066272:CDS |
| CATTGATCCAGTACGTGTAG+TGG | 0.602340 | 1.1:-71778715 | MS.gene066272:CDS |
| CCTCTTCAACGCTTATGGCA+CGG | 0.607325 | 1.1:+71778493 | None:intergenic |
| GGTGTACCATAGAGCACGAA+AGG | 0.612408 | 1.1:+71778561 | None:intergenic |
| TTATGGAAGCTACTGAGCTG+AGG | 0.657868 | 1.1:+71779383 | None:intergenic |
| CTTCAACGCTTATGGCACGG+TGG | 0.735189 | 1.1:+71778496 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAGATGATAAAAAGA+AGG | - | chr1.1:71779241-71779260 | MS.gene066272:intron | 15.0% |
| !! | TTTAGATTAATAAAGTGTAT+TGG | - | chr1.1:71778927-71778946 | MS.gene066272:intron | 15.0% |
| !!! | TTCTTTTTATCATCTTTTTT+AGG | + | chr1.1:71779242-71779261 | None:intergenic | 15.0% |
| !! | ATAAAAAGTGGTATTAATCA+AGG | + | chr1.1:71778532-71778551 | None:intergenic | 20.0% |
| !! | ATTCATCTGATGATTATAAT+AGG | + | chr1.1:71779330-71779349 | None:intergenic | 20.0% |
| !! | TAAAATATCGAACTTGAAAT+CGG | + | chr1.1:71778911-71778930 | None:intergenic | 20.0% |
| !! | TAGAAATTGTTCAAAAGAAA+AGG | + | chr1.1:71778592-71778611 | None:intergenic | 20.0% |
| !! | TTGATAAAGAAGATAAAAAG+TGG | + | chr1.1:71778544-71778563 | None:intergenic | 20.0% |
| !!! | AAGTATTGATTCTATAGATT+TGG | + | chr1.1:71779015-71779034 | None:intergenic | 20.0% |
| ! | AAAATATCGAACTTGAAATC+GGG | + | chr1.1:71778910-71778929 | None:intergenic | 25.0% |
| ! | ACGAAAGGATTAATTATCAT+TGG | + | chr1.1:71779287-71779306 | None:intergenic | 25.0% |
| ! | TACTAACCTTCATATTGTTA+TGG | + | chr1.1:71778497-71778516 | None:intergenic | 25.0% |
| ! | TAGTTAATTCAATACCTGAA+AGG | + | chr1.1:71778779-71778798 | None:intergenic | 25.0% |
| ! | TATCAATGCTAAAGCATAAT+TGG | - | chr1.1:71778558-71778577 | MS.gene066272:CDS | 25.0% |
| ! | TGATGATTATAATAGGTAGT+AGG | + | chr1.1:71779323-71779342 | None:intergenic | 25.0% |
| ! | TTGGAAATTGAAAATCTGAT+TGG | - | chr1.1:71778946-71778965 | MS.gene066272:intron | 25.0% |
| !! | TTGAATTTGAAGTAGTTCTT+TGG | - | chr1.1:71778614-71778633 | MS.gene066272:CDS | 25.0% |
| GACAAGAAAATGACCTTAAT+AGG | - | chr1.1:71779119-71779138 | MS.gene066272:CDS | 30.0% | |
| GTATTTGCAATATTGTTGCA+GGG | - | chr1.1:71779069-71779088 | MS.gene066272:intron | 30.0% | |
| TAGCTTCCATAACAATATGA+AGG | - | chr1.1:71778488-71778507 | MS.gene066272:CDS | 30.0% | |
| TATTTGCAATATTGTTGCAG+GGG | - | chr1.1:71779070-71779089 | MS.gene066272:intron | 30.0% | |
| TGTATTTGCAATATTGTTGC+AGG | - | chr1.1:71779068-71779087 | MS.gene066272:intron | 30.0% | |
| ACAGAAAGTTGTGTTGAAGT+TGG | - | chr1.1:71778704-71778723 | MS.gene066272:CDS | 35.0% | |
| ATTAATCCTTTCGTGCTCTA+TGG | - | chr1.1:71779293-71779312 | MS.gene066272:intron | 35.0% | |
| CATGCTGAAATACTTTCAGT+TGG | - | chr1.1:71779188-71779207 | MS.gene066272:intron | 35.0% | |
| TAGATGTGGATGATTGTGTA+TGG | - | chr1.1:71778975-71778994 | MS.gene066272:intron | 35.0% | |
| TGGATCAATGTCTCCTATTA+AGG | + | chr1.1:71779135-71779154 | None:intergenic | 35.0% | |
| ! | TCTTTCTTTTCCTCTTTAGC+TGG | + | chr1.1:71779215-71779234 | None:intergenic | 35.0% |
| AAAGCTATGAAAGCAGTCTC+TGG | - | chr1.1:71778753-71778772 | MS.gene066272:CDS | 40.0% | |
| CTGATTGGACAGTTTAGATG+TGG | - | chr1.1:71778961-71778980 | MS.gene066272:intron | 40.0% | |
| TAGATGATGACACAGCCATT+AGG | + | chr1.1:71779395-71779414 | None:intergenic | 40.0% | |
| AACATTTCAGACGTGACCGA+CGG | + | chr1.1:71778883-71778902 | None:intergenic | 45.0% | |
| AGGATCCTCTTCAACGCTTA+TGG | + | chr1.1:71779375-71779394 | None:intergenic | 45.0% | |
| ATCTCGTGACTCTATACCGT+CGG | - | chr1.1:71778864-71778883 | MS.gene066272:intron | 45.0% | |
| CATTGATCCAGTACGTGTAG+TGG | - | chr1.1:71779145-71779164 | MS.gene066272:CDS | 45.0% | |
| GCACGGTGGTATGAATTGTA+TGG | + | chr1.1:71779353-71779372 | None:intergenic | 45.0% | |
| TACGTGTAGTGGCTAAGCTA+AGG | - | chr1.1:71779156-71779175 | MS.gene066272:intron | 45.0% | |
| TTATGGAAGCTACTGAGCTG+AGG | + | chr1.1:71778480-71778499 | None:intergenic | 45.0% | |
| TTCAGTTGGACCAGCTAAAG+AGG | - | chr1.1:71779202-71779221 | MS.gene066272:intron | 45.0% | |
| ! | AGCGTTGAAGAGGATCCTAA+TGG | - | chr1.1:71779377-71779396 | MS.gene066272:CDS | 45.0% |
| AGCTTAGCCACTACACGTAC+TGG | + | chr1.1:71779155-71779174 | None:intergenic | 50.0% | |
| CCTCTTCAACGCTTATGGCA+CGG | + | chr1.1:71779370-71779389 | None:intergenic | 50.0% | |
| GGTGTACCATAGAGCACGAA+AGG | + | chr1.1:71779302-71779321 | None:intergenic | 50.0% | |
| ! | AAAGCAGTCTCTGGCCTTTC+AGG | - | chr1.1:71778762-71778781 | MS.gene066272:CDS | 50.0% |
| CTTCAACGCTTATGGCACGG+TGG | + | chr1.1:71779367-71779386 | None:intergenic | 55.0% | |
| !! | CCGTGCCATAAGCGTTGAAG+AGG | - | chr1.1:71779367-71779386 | MS.gene066272:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 71778467 | 71779415 | 71778467 | ID=MS.gene066272 |
| chr1.1 | mRNA | 71778467 | 71779415 | 71778467 | ID=MS.gene066272.t1;Parent=MS.gene066272 |
| chr1.1 | exon | 71779373 | 71779415 | 71779373 | ID=MS.gene066272.t1.exon1;Parent=MS.gene066272.t1 |
| chr1.1 | CDS | 71779373 | 71779415 | 71779373 | ID=cds.MS.gene066272.t1;Parent=MS.gene066272.t1 |
| chr1.1 | exon | 71779099 | 71779174 | 71779099 | ID=MS.gene066272.t1.exon2;Parent=MS.gene066272.t1 |
| chr1.1 | CDS | 71779099 | 71779174 | 71779099 | ID=cds.MS.gene066272.t1;Parent=MS.gene066272.t1 |
| chr1.1 | exon | 71778467 | 71778792 | 71778467 | ID=MS.gene066272.t1.exon3;Parent=MS.gene066272.t1 |
| chr1.1 | CDS | 71778467 | 71778792 | 71778467 | ID=cds.MS.gene066272.t1;Parent=MS.gene066272.t1 |
| Gene Sequence |
| Protein sequence |
