Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066856.t1 | XP_013444538.1 | 99.4 | 157 | 1 | 0 | 1 | 157 | 181 | 337 | 3.80E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066856.t1 | O81037 | 57.8 | 161 | 59 | 4 | 1 | 157 | 193 | 348 | 2.8e-39 | 162.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066856.t1 | A0A072TNY8 | 99.4 | 157 | 1 | 0 | 1 | 157 | 181 | 337 | 2.7e-75 | 290.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene066856.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene066856 | 0.801633 | 8.21E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene066856 | 0.84411 | 9.26E-59 | -1.69E-46 |
| MS.gene050128 | MS.gene066856 | 0.804912 | 1.72E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene066856 | 0.806866 | 6.68E-50 | -1.69E-46 |
| MS.gene051626 | MS.gene066856 | 0.823709 | 1.20E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene066856 | 0.809956 | 1.46E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene066856 | 0.827962 | 1.17E-54 | -1.69E-46 |
| MS.gene051789 | MS.gene066856 | 0.818806 | 1.62E-52 | -1.69E-46 |
| MS.gene052178 | MS.gene066856 | 0.804599 | 2.00E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene066856 | 0.826621 | 2.45E-54 | -1.69E-46 |
| MS.gene053074 | MS.gene066856 | 0.811084 | 8.36E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene066856 | 0.826126 | 3.22E-54 | -1.69E-46 |
| MS.gene054184 | MS.gene066856 | 0.820031 | 8.50E-53 | -1.69E-46 |
| MS.gene054185 | MS.gene066856 | 0.827506 | 1.51E-54 | -1.69E-46 |
| MS.gene054542 | MS.gene066856 | 0.808758 | 2.65E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene066856 | 0.85569 | 5.32E-62 | -1.69E-46 |
| MS.gene055390 | MS.gene066856 | 0.808113 | 3.63E-50 | -1.69E-46 |
| MS.gene055422 | MS.gene066856 | 0.810444 | 1.15E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene066856 | -0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene057754 | MS.gene066856 | 0.842868 | 1.99E-58 | -1.69E-46 |
| MS.gene057794 | MS.gene066856 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene066856 | 0.803597 | 3.23E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene066856 | 0.800438 | 1.44E-48 | -1.69E-46 |
| MS.gene058182 | MS.gene066856 | 0.806339 | 8.63E-50 | -1.69E-46 |
| MS.gene058845 | MS.gene066856 | 0.811372 | 7.23E-51 | -1.69E-46 |
| MS.gene059124 | MS.gene066856 | 0.801727 | 7.85E-49 | -1.69E-46 |
| MS.gene059421 | MS.gene066856 | 0.813598 | 2.36E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene066856 | 0.812795 | 3.54E-51 | -1.69E-46 |
| MS.gene060320 | MS.gene066856 | 0.803886 | 2.81E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene066856 | 0.815506 | 8.93E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene066856 | 0.883856 | 3.15E-71 | -1.69E-46 |
| MS.gene061049 | MS.gene066856 | 0.804961 | 1.68E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene066856 | 0.809831 | 1.56E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066856.t1 | MTR_8g024210 | 99.363 | 157 | 1 | 0 | 1 | 157 | 181 | 337 | 2.15e-109 | 316 |
| MS.gene066856.t1 | MTR_3g099620 | 52.023 | 173 | 67 | 3 | 1 | 157 | 116 | 288 | 9.65e-55 | 175 |
| MS.gene066856.t1 | MTR_0194s0050 | 55.429 | 175 | 60 | 4 | 1 | 157 | 184 | 358 | 4.73e-53 | 172 |
| MS.gene066856.t1 | MTR_8g066770 | 50.888 | 169 | 68 | 3 | 1 | 157 | 114 | 279 | 7.51e-51 | 165 |
| MS.gene066856.t1 | MTR_7g113830 | 52.096 | 167 | 70 | 2 | 1 | 157 | 116 | 282 | 1.74e-49 | 161 |
| MS.gene066856.t1 | MTR_1g082480 | 50.292 | 171 | 71 | 3 | 1 | 157 | 136 | 306 | 4.96e-49 | 160 |
| MS.gene066856.t1 | MTR_1g082480 | 50.292 | 171 | 71 | 3 | 1 | 157 | 136 | 306 | 6.54e-49 | 160 |
| MS.gene066856.t1 | MTR_5g064950 | 47.126 | 174 | 70 | 4 | 1 | 157 | 100 | 268 | 2.10e-47 | 155 |
| MS.gene066856.t1 | MTR_5g064950 | 47.126 | 174 | 70 | 4 | 1 | 157 | 100 | 268 | 2.96e-47 | 155 |
| MS.gene066856.t1 | MTR_2g022280 | 44.186 | 172 | 74 | 2 | 1 | 157 | 1 | 165 | 2.91e-40 | 134 |
| MS.gene066856.t1 | MTR_3g090850 | 40.191 | 209 | 72 | 5 | 1 | 157 | 101 | 308 | 7.20e-36 | 126 |
| MS.gene066856.t1 | MTR_1g028470 | 36.022 | 186 | 90 | 2 | 1 | 157 | 87 | 272 | 2.25e-31 | 114 |
| MS.gene066856.t1 | MTR_1g072320 | 30.282 | 142 | 92 | 3 | 3 | 139 | 471 | 610 | 2.55e-14 | 69.7 |
| MS.gene066856.t1 | MTR_8g027495 | 27.160 | 162 | 95 | 5 | 1 | 157 | 457 | 600 | 9.65e-14 | 67.8 |
| MS.gene066856.t1 | MTR_5g014520 | 29.530 | 149 | 100 | 2 | 3 | 151 | 158 | 301 | 3.44e-13 | 65.9 |
| MS.gene066856.t1 | MTR_3g030700 | 28.025 | 157 | 101 | 4 | 4 | 154 | 198 | 348 | 2.90e-12 | 63.5 |
| MS.gene066856.t1 | MTR_4g094762 | 28.758 | 153 | 86 | 4 | 3 | 141 | 293 | 436 | 3.96e-11 | 60.5 |
| MS.gene066856.t1 | MTR_1g112680 | 28.333 | 180 | 96 | 6 | 3 | 154 | 318 | 492 | 5.12e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066856.t1 | AT2G46810 | 57.764 | 161 | 59 | 4 | 1 | 157 | 121 | 276 | 1.12e-54 | 174 |
| MS.gene066856.t1 | AT2G46810 | 57.764 | 161 | 59 | 4 | 1 | 157 | 121 | 276 | 2.49e-54 | 174 |
| MS.gene066856.t1 | AT2G46810 | 57.764 | 161 | 59 | 4 | 1 | 157 | 181 | 336 | 4.31e-54 | 174 |
| MS.gene066856.t1 | AT2G46810 | 57.764 | 161 | 59 | 4 | 1 | 157 | 193 | 348 | 6.50e-54 | 174 |
| MS.gene066856.t1 | AT3G24140 | 51.977 | 177 | 65 | 2 | 1 | 157 | 197 | 373 | 1.38e-49 | 164 |
| MS.gene066856.t1 | AT3G24140 | 51.977 | 177 | 65 | 2 | 1 | 157 | 197 | 373 | 1.70e-49 | 164 |
| MS.gene066856.t1 | AT4G01460 | 50.296 | 169 | 68 | 3 | 1 | 157 | 115 | 279 | 1.28e-48 | 159 |
| MS.gene066856.t1 | AT4G01460 | 50.296 | 169 | 68 | 3 | 1 | 157 | 115 | 279 | 1.28e-48 | 159 |
| MS.gene066856.t1 | AT1G22490 | 50.595 | 168 | 66 | 3 | 1 | 157 | 115 | 276 | 1.98e-48 | 158 |
| MS.gene066856.t1 | AT1G22490 | 50.595 | 168 | 66 | 3 | 1 | 157 | 115 | 276 | 9.81e-48 | 157 |
| MS.gene066856.t1 | AT1G72210 | 48.795 | 166 | 75 | 3 | 1 | 157 | 125 | 289 | 1.74e-46 | 154 |
| MS.gene066856.t1 | AT1G72210 | 48.795 | 166 | 75 | 3 | 1 | 157 | 125 | 289 | 2.43e-46 | 153 |
| MS.gene066856.t1 | AT3G61950 | 53.125 | 160 | 67 | 3 | 1 | 157 | 127 | 281 | 8.62e-46 | 151 |
| MS.gene066856.t1 | AT3G61950 | 53.125 | 160 | 67 | 3 | 1 | 157 | 127 | 281 | 1.61e-45 | 151 |
| MS.gene066856.t1 | AT3G61950 | 53.125 | 160 | 67 | 3 | 1 | 157 | 178 | 332 | 4.45e-45 | 151 |
| MS.gene066856.t1 | AT3G61950 | 53.125 | 160 | 67 | 3 | 1 | 157 | 178 | 332 | 7.73e-45 | 150 |
| MS.gene066856.t1 | AT3G06120 | 43.023 | 172 | 83 | 2 | 1 | 157 | 1 | 172 | 9.53e-40 | 133 |
| MS.gene066856.t1 | AT5G46690 | 44.149 | 188 | 74 | 5 | 1 | 157 | 88 | 275 | 4.24e-34 | 121 |
| MS.gene066856.t1 | AT5G46690 | 44.149 | 188 | 74 | 5 | 1 | 157 | 88 | 275 | 1.67e-33 | 120 |
| MS.gene066856.t1 | AT5G65320 | 39.766 | 171 | 84 | 4 | 1 | 157 | 102 | 267 | 1.32e-29 | 109 |
| MS.gene066856.t1 | AT5G65320 | 40.361 | 166 | 90 | 3 | 1 | 157 | 102 | 267 | 2.95e-29 | 108 |
| MS.gene066856.t1 | AT5G53210 | 66.667 | 63 | 21 | 0 | 1 | 63 | 102 | 164 | 3.27e-25 | 99.0 |
| MS.gene066856.t1 | AT5G53210 | 66.667 | 63 | 21 | 0 | 1 | 63 | 102 | 164 | 3.64e-25 | 99.4 |
| MS.gene066856.t1 | AT1G12860 | 31.677 | 161 | 93 | 5 | 3 | 154 | 268 | 420 | 3.68e-13 | 66.2 |
| MS.gene066856.t1 | AT1G12860 | 31.677 | 161 | 93 | 5 | 3 | 154 | 268 | 420 | 3.77e-13 | 66.2 |
| MS.gene066856.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 3 | 154 | 308 | 464 | 5.14e-13 | 65.9 |
| MS.gene066856.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 3 | 154 | 308 | 464 | 5.50e-13 | 65.9 |
| MS.gene066856.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 3 | 154 | 308 | 464 | 5.50e-13 | 65.9 |
| MS.gene066856.t1 | AT3G26744 | 31.098 | 164 | 94 | 5 | 3 | 154 | 308 | 464 | 5.50e-13 | 65.9 |
| MS.gene066856.t1 | AT2G16910 | 29.213 | 178 | 97 | 6 | 3 | 157 | 315 | 486 | 2.10e-12 | 64.3 |
Find 24 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTGCTTTGGAAAATCTT+AGG | 0.306717 | 8.1:+76120335 | MS.gene066856:CDS |
| GATAAAGGTTATTGTTGCTT+TGG | 0.325174 | 8.1:+76120324 | MS.gene066856:CDS |
| GGTGACCAAGCATCAATAAT+TGG | 0.351169 | 8.1:+76120049 | MS.gene066856:CDS |
| ACTCAAAAGATGTGATGTTT+AGG | 0.363568 | 8.1:-76120372 | None:intergenic |
| ATGACCACCTTAGTGTTCTT+AGG | 0.380570 | 8.1:+76119604 | MS.gene066856:CDS |
| CACTAAGGTGGTCATTCATT+TGG | 0.381725 | 8.1:-76119596 | None:intergenic |
| CTCCTCCAATTATTGATGCT+TGG | 0.405749 | 8.1:-76120054 | None:intergenic |
| AAAGGCTGGTCAATTGATAA+AGG | 0.417902 | 8.1:+76120309 | MS.gene066856:CDS |
| TCATCATCACCGCCTAGTTA+TGG | 0.434743 | 8.1:+76120169 | MS.gene066856:CDS |
| GAATAATACCTTGAGATTGA+AGG | 0.480462 | 8.1:-76120410 | None:intergenic |
| ATATTGCTGTTGAACGCAAT+AGG | 0.480469 | 8.1:+76119568 | MS.gene066856:CDS |
| TGCCATCTTCATATGTTCAA+AGG | 0.494987 | 8.1:+76119634 | MS.gene066856:CDS |
| GATAATTTCATTCCATAACT+AGG | 0.502981 | 8.1:-76120181 | None:intergenic |
| GCCATCTTCATATGTTCAAA+GGG | 0.503887 | 8.1:+76119635 | MS.gene066856:CDS |
| TTGTGAAGAAAATGAGGTAA+AGG | 0.510975 | 8.1:+76120207 | MS.gene066856:CDS |
| ACCCTTTGAACATATGAAGA+TGG | 0.543479 | 8.1:-76119636 | None:intergenic |
| ATCACCTTGTGAAGAAAATG+AGG | 0.546176 | 8.1:+76120201 | MS.gene066856:CDS |
| TGTGATGTTTAGGTGAAGAA+TGG | 0.555251 | 8.1:-76120362 | None:intergenic |
| ACTCTACTCCTTCAATCTCA+AGG | 0.617899 | 8.1:+76120402 | MS.gene066856:CDS |
| TGAGAGACCTAAGAACACTA+AGG | 0.629812 | 8.1:-76119611 | None:intergenic |
| GACCAAGCATCAATAATTGG+AGG | 0.640978 | 8.1:+76120052 | MS.gene066856:CDS |
| AGGCGGTGATGATGATGATG+AGG | 0.686258 | 8.1:-76120161 | None:intergenic |
| GAGACCTAAGAACACTAAGG+TGG | 0.704555 | 8.1:-76119608 | None:intergenic |
| AATTTCATTCCATAACTAGG+CGG | 0.718157 | 8.1:-76120178 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAAAAGAATGAAGATTT+TGG | + | chr8.1:76120136-76120155 | MS.gene066856:CDS | 15.0% |
| !!! | TTATGTTTTAAAAATCACTT+AGG | + | chr8.1:76119985-76120004 | MS.gene066856:intron | 15.0% |
| !! | TATACAAAACCAAAAAAGAT+TGG | - | chr8.1:76119756-76119775 | None:intergenic | 20.0% |
| !! | TGTTTAATGTTTATGTGATA+GGG | + | chr8.1:76120028-76120047 | MS.gene066856:intron | 20.0% |
| !! | TTGTTTAATGTTTATGTGAT+AGG | + | chr8.1:76120027-76120046 | MS.gene066856:intron | 20.0% |
| !!! | GTTAAAAATCCAATCTTTTT+TGG | + | chr8.1:76119744-76119763 | MS.gene066856:intron | 20.0% |
| !!! | TTTTTTGGTTTTGTATACAA+GGG | + | chr8.1:76119759-76119778 | MS.gene066856:intron | 20.0% |
| ! | ACATGAAAATCTCAAAAGAA+AGG | - | chr8.1:76119726-76119745 | None:intergenic | 25.0% |
| ! | GAAAATTGAGTGCAAAAAAA+AGG | + | chr8.1:76120291-76120310 | MS.gene066856:CDS | 25.0% |
| ! | GATAATTTCATTCCATAACT+AGG | - | chr8.1:76120184-76120203 | None:intergenic | 25.0% |
| !!! | AAGAAAGGATTTTGAAGAAA+AGG | - | chr8.1:76119711-76119730 | None:intergenic | 25.0% |
| !!! | AATTGATTTTGTGAAAGAGT+TGG | + | chr8.1:76120078-76120097 | MS.gene066856:CDS | 25.0% |
| !!! | AGAAAGGATTTTGAAGAAAA+GGG | - | chr8.1:76119710-76119729 | None:intergenic | 25.0% |
| !!! | CTTTTTTGGTTTTGTATACA+AGG | + | chr8.1:76119758-76119777 | MS.gene066856:intron | 25.0% |
| AATCTCTTGAAGCACAAAAA+AGG | + | chr8.1:76120113-76120132 | MS.gene066856:CDS | 30.0% | |
| AATTTCATTCCATAACTAGG+CGG | - | chr8.1:76120181-76120200 | None:intergenic | 30.0% | |
| ACTCAAAAGATGTGATGTTT+AGG | - | chr8.1:76120375-76120394 | None:intergenic | 30.0% | |
| TTGTGAAGAAAATGAGGTAA+AGG | + | chr8.1:76120207-76120226 | MS.gene066856:CDS | 30.0% | |
| ! | GATAAAGGTTATTGTTGCTT+TGG | + | chr8.1:76120324-76120343 | MS.gene066856:CDS | 30.0% |
| ! | TTTACCTCATTTTCTTCACA+AGG | - | chr8.1:76120208-76120227 | None:intergenic | 30.0% |
| !! | TTGTTGCTTTGGAAAATCTT+AGG | + | chr8.1:76120335-76120354 | MS.gene066856:CDS | 30.0% |
| !!! | ACTCAATTTTCAAGTTCACA+TGG | - | chr8.1:76120282-76120301 | None:intergenic | 30.0% |
| !!! | CTCAATTTTCAAGTTCACAT+GGG | - | chr8.1:76120281-76120300 | None:intergenic | 30.0% |
| ACCCTTTGAACATATGAAGA+TGG | - | chr8.1:76119639-76119658 | None:intergenic | 35.0% | |
| ATCACCTTGTGAAGAAAATG+AGG | + | chr8.1:76120201-76120220 | MS.gene066856:CDS | 35.0% | |
| ATTGAGTGCAAAAAAAAGGC+TGG | + | chr8.1:76120295-76120314 | MS.gene066856:CDS | 35.0% | |
| GCCATCTTCATATGTTCAAA+GGG | + | chr8.1:76119635-76119654 | MS.gene066856:CDS | 35.0% | |
| TGCCATCTTCATATGTTCAA+AGG | + | chr8.1:76119634-76119653 | MS.gene066856:CDS | 35.0% | |
| TGTGATGTTTAGGTGAAGAA+TGG | - | chr8.1:76120365-76120384 | None:intergenic | 35.0% | |
| ! | AAAGGCTGGTCAATTGATAA+AGG | + | chr8.1:76120309-76120328 | MS.gene066856:CDS | 35.0% |
| ! | ATATTGCTGTTGAACGCAAT+AGG | + | chr8.1:76119568-76119587 | MS.gene066856:CDS | 35.0% |
| ! | TTTGTATACAAGGGCATGTT+TGG | + | chr8.1:76119768-76119787 | MS.gene066856:intron | 35.0% |
| ATGACCACCTTAGTGTTCTT+AGG | + | chr8.1:76119604-76119623 | MS.gene066856:CDS | 40.0% | |
| CACTAAGGTGGTCATTCATT+TGG | - | chr8.1:76119599-76119618 | None:intergenic | 40.0% | |
| CTCCTCCAATTATTGATGCT+TGG | - | chr8.1:76120057-76120076 | None:intergenic | 40.0% | |
| GACCAAGCATCAATAATTGG+AGG | + | chr8.1:76120052-76120071 | MS.gene066856:CDS | 40.0% | |
| GGTGACCAAGCATCAATAAT+TGG | + | chr8.1:76120049-76120068 | MS.gene066856:CDS | 40.0% | |
| TGAGAGACCTAAGAACACTA+AGG | - | chr8.1:76119614-76119633 | None:intergenic | 40.0% | |
| GAGACCTAAGAACACTAAGG+TGG | - | chr8.1:76119611-76119630 | None:intergenic | 45.0% | |
| TCATCATCACCGCCTAGTTA+TGG | + | chr8.1:76120169-76120188 | MS.gene066856:CDS | 45.0% | |
| ! | AGGCGGTGATGATGATGATG+AGG | - | chr8.1:76120164-76120183 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 76119561 | 76120423 | 76119561 | ID=MS.gene066856 |
| chr8.1 | mRNA | 76119561 | 76120423 | 76119561 | ID=MS.gene066856.t1;Parent=MS.gene066856 |
| chr8.1 | exon | 76119561 | 76119656 | 76119561 | ID=MS.gene066856.t1.exon1;Parent=MS.gene066856.t1 |
| chr8.1 | CDS | 76119561 | 76119656 | 76119561 | ID=cds.MS.gene066856.t1;Parent=MS.gene066856.t1 |
| chr8.1 | exon | 76120049 | 76120423 | 76120049 | ID=MS.gene066856.t1.exon2;Parent=MS.gene066856.t1 |
| chr8.1 | CDS | 76120049 | 76120423 | 76120049 | ID=cds.MS.gene066856.t1;Parent=MS.gene066856.t1 |
| Gene Sequence |
| Protein sequence |
