Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067233.t1 | XP_013445304.1 | 98.3 | 412 | 7 | 0 | 1 | 412 | 1 | 412 | 2.20E-228 | 801.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067233.t1 | O64517 | 65.1 | 424 | 130 | 6 | 1 | 412 | 1 | 418 | 1.4e-149 | 530.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067233.t1 | A0A072U0M2 | 98.3 | 412 | 7 | 0 | 1 | 412 | 1 | 412 | 1.6e-228 | 801.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049807 | MS.gene067233 | 0.831253 | 1.85E-55 | -1.69E-46 |
| MS.gene04998 | MS.gene067233 | 0.810681 | 1.02E-50 | -1.69E-46 |
| MS.gene050359 | MS.gene067233 | 0.819616 | 1.06E-52 | -1.69E-46 |
| MS.gene050414 | MS.gene067233 | 0.838868 | 2.23E-57 | -1.69E-46 |
| MS.gene050646 | MS.gene067233 | 0.823735 | 1.18E-53 | -1.69E-46 |
| MS.gene051003 | MS.gene067233 | 0.803877 | 2.83E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene067233 | 0.807526 | 4.84E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene067233 | 0.807284 | 5.45E-50 | -1.69E-46 |
| MS.gene052239 | MS.gene067233 | -0.841269 | 5.26E-58 | -1.69E-46 |
| MS.gene052242 | MS.gene067233 | -0.846926 | 1.60E-59 | -1.69E-46 |
| MS.gene052307 | MS.gene067233 | 0.800992 | 1.11E-48 | -1.69E-46 |
| MS.gene052335 | MS.gene067233 | 0.82659 | 2.50E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene067233 | 0.804592 | 2.01E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene067233 | 0.803523 | 3.35E-49 | -1.69E-46 |
| MS.gene053143 | MS.gene067233 | 0.822821 | 1.93E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene067233 | 0.801931 | 7.13E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene067233 | 0.805663 | 1.20E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene067233 | 0.804653 | 1.95E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene067233 | 0.801947 | 7.08E-49 | -1.69E-46 |
| MS.gene056677 | MS.gene067233 | 0.838122 | 3.47E-57 | -1.69E-46 |
| MS.gene057102 | MS.gene067233 | 0.808688 | 2.74E-50 | -1.69E-46 |
| MS.gene057576 | MS.gene067233 | 0.803339 | 3.65E-49 | -1.69E-46 |
| MS.gene058330 | MS.gene067233 | 0.825571 | 4.36E-54 | -1.69E-46 |
| MS.gene059170 | MS.gene067233 | 0.815812 | 7.63E-52 | -1.69E-46 |
| MS.gene059965 | MS.gene067233 | 0.818796 | 1.63E-52 | -1.69E-46 |
| MS.gene060259 | MS.gene067233 | 0.813538 | 2.43E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene067233 | 0.803668 | 3.12E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene067233 | 0.866414 | 2.90E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067233.t1 | MTR_8g447080 | 98.301 | 412 | 7 | 0 | 1 | 412 | 1 | 412 | 0.0 | 835 |
| MS.gene067233.t1 | MTR_2g063570 | 66.187 | 417 | 117 | 6 | 1 | 412 | 4 | 401 | 0.0 | 540 |
| MS.gene067233.t1 | MTR_7g088035 | 54.140 | 157 | 68 | 2 | 3 | 156 | 7 | 162 | 2.26e-49 | 170 |
| MS.gene067233.t1 | MTR_7g088035 | 51.807 | 83 | 40 | 0 | 329 | 411 | 231 | 313 | 1.11e-21 | 95.1 |
| MS.gene067233.t1 | MTR_0340s0030 | 31.765 | 170 | 100 | 2 | 3 | 158 | 4 | 171 | 1.01e-23 | 100 |
| MS.gene067233.t1 | MTR_7g451400 | 37.333 | 150 | 87 | 4 | 3 | 146 | 74 | 222 | 1.09e-23 | 101 |
| MS.gene067233.t1 | MTR_2g008990 | 36.913 | 149 | 89 | 3 | 3 | 146 | 116 | 264 | 7.66e-20 | 91.3 |
| MS.gene067233.t1 | MTR_2g008990 | 36.913 | 149 | 89 | 3 | 3 | 146 | 116 | 264 | 8.75e-20 | 90.9 |
| MS.gene067233.t1 | MTR_2g008990 | 36.913 | 149 | 89 | 3 | 3 | 146 | 116 | 264 | 9.22e-20 | 90.9 |
| MS.gene067233.t1 | MTR_4g094202 | 35.526 | 152 | 89 | 4 | 2 | 146 | 100 | 249 | 3.63e-19 | 89.0 |
| MS.gene067233.t1 | MTR_2g008970 | 33.775 | 151 | 92 | 3 | 3 | 146 | 88 | 237 | 3.66e-18 | 85.9 |
| MS.gene067233.t1 | MTR_2g008970 | 33.775 | 151 | 92 | 3 | 3 | 146 | 88 | 237 | 9.43e-18 | 84.0 |
| MS.gene067233.t1 | MTR_4g094210 | 34.228 | 149 | 92 | 4 | 3 | 146 | 116 | 263 | 5.82e-17 | 82.4 |
| MS.gene067233.t1 | MTR_2g008980 | 31.788 | 151 | 95 | 5 | 3 | 146 | 110 | 259 | 2.89e-15 | 75.9 |
| MS.gene067233.t1 | MTR_2g008980 | 31.788 | 151 | 95 | 5 | 3 | 146 | 110 | 259 | 5.13e-15 | 76.6 |
| MS.gene067233.t1 | MTR_2g008980 | 31.788 | 151 | 95 | 5 | 3 | 146 | 110 | 259 | 6.11e-15 | 76.3 |
| MS.gene067233.t1 | MTR_2g008980 | 31.788 | 151 | 95 | 5 | 3 | 146 | 110 | 259 | 1.05e-14 | 75.1 |
| MS.gene067233.t1 | MTR_1g054545 | 34.545 | 110 | 66 | 2 | 3 | 106 | 16 | 125 | 7.32e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067233.t1 | AT1G79340 | 66.274 | 424 | 125 | 6 | 1 | 412 | 1 | 418 | 0.0 | 552 |
| MS.gene067233.t1 | AT1G79330 | 65.394 | 419 | 129 | 5 | 1 | 412 | 1 | 410 | 0.0 | 549 |
| MS.gene067233.t1 | AT1G79310 | 55.924 | 422 | 157 | 9 | 1 | 412 | 1 | 403 | 5.77e-159 | 454 |
| MS.gene067233.t1 | AT1G79320 | 57.729 | 414 | 127 | 6 | 1 | 412 | 1 | 368 | 4.03e-158 | 450 |
| MS.gene067233.t1 | AT1G16420 | 41.801 | 433 | 178 | 10 | 1 | 412 | 1 | 380 | 1.90e-98 | 299 |
| MS.gene067233.t1 | AT5G04200 | 36.386 | 415 | 156 | 11 | 3 | 411 | 10 | 322 | 1.39e-72 | 231 |
| MS.gene067233.t1 | AT1G02170 | 37.584 | 149 | 87 | 4 | 3 | 146 | 80 | 227 | 6.49e-24 | 102 |
| MS.gene067233.t1 | AT4G25110 | 36.486 | 148 | 89 | 3 | 3 | 146 | 116 | 262 | 7.96e-23 | 100 |
| MS.gene067233.t1 | AT4G25110 | 36.242 | 149 | 89 | 4 | 3 | 146 | 116 | 263 | 5.75e-22 | 97.4 |
| MS.gene067233.t1 | AT5G64240 | 33.557 | 149 | 93 | 3 | 3 | 146 | 90 | 237 | 1.85e-17 | 83.6 |
| MS.gene067233.t1 | AT5G64240 | 33.557 | 149 | 93 | 3 | 3 | 146 | 90 | 237 | 2.29e-17 | 82.8 |
Find 120 sgRNAs with CRISPR-Local
Find 150 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATATACTTAAGCAGAAAAC+TGG | 0.288159 | 8.1:-58925438 | MS.gene067233:CDS |
| ATAAGCATTAGCTGCATTTC+CGG | 0.302115 | 8.1:+58925065 | None:intergenic |
| TTCATAAACTTCTTCACTTT+AGG | 0.312797 | 8.1:+58925348 | None:intergenic |
| GGATCTGGCTTTGGATTGTC+TGG | 0.324284 | 8.1:-58925624 | MS.gene067233:CDS |
| ATGAAGAAACAAATGAAGAT+AGG | 0.350793 | 8.1:-58925518 | MS.gene067233:CDS |
| GAACATTCTGGATCTGGCTT+TGG | 0.359410 | 8.1:-58925633 | MS.gene067233:CDS |
| GTGCTGTTTGTTCATTATAG+TGG | 0.359968 | 8.1:-58926446 | MS.gene067233:CDS |
| AGGGGATTTCATGTCCCTTC+AGG | 0.372762 | 8.1:-58925564 | MS.gene067233:CDS |
| GGAAATGCAGCTAATGCTTA+TGG | 0.373990 | 8.1:-58925063 | MS.gene067233:CDS |
| AGGTGGACAAGGTGGGATAC+TGG | 0.379608 | 8.1:-58925289 | MS.gene067233:CDS |
| TTTCTTCATCCCTGCGTGTA+TGG | 0.408613 | 8.1:+58925532 | None:intergenic |
| AAGGGAGAAGAGGAACATTC+TGG | 0.411416 | 8.1:-58925645 | MS.gene067233:CDS |
| CCCACCTCTGTCTCCATGGC+AGG | 0.417075 | 8.1:+58925195 | None:intergenic |
| GACAAGCATCTGCTGAAGTC+TGG | 0.432339 | 8.1:+58925091 | None:intergenic |
| TCATCGTACACGGAGCCAAC+AGG | 0.436679 | 8.1:-58926524 | MS.gene067233:CDS |
| CACCTTCACCACCTCCATGC+TGG | 0.437332 | 8.1:+58925307 | None:intergenic |
| CAAAACAGTGATGTCATCTT+CGG | 0.443836 | 8.1:+58926559 | None:intergenic |
| GAAGAGGAACATTCTGGATC+TGG | 0.447705 | 8.1:-58925639 | MS.gene067233:CDS |
| AGCTTTCAGCAATGCAATAC+AGG | 0.451961 | 8.1:-58925040 | MS.gene067233:CDS |
| ACTCGTCTTCCTGCTGAAAC+CGG | 0.458013 | 8.1:-58926416 | MS.gene067233:CDS |
| TGATGATTTCAAGGAATTTG+TGG | 0.462771 | 8.1:-58925766 | MS.gene067233:CDS |
| AGTCTTATGTTCTTTCCTGT+TGG | 0.463520 | 8.1:+58926509 | None:intergenic |
| TCAGCAGATGCTTGTCCTGC+CGG | 0.466394 | 8.1:-58925084 | MS.gene067233:CDS |
| GATTGTCTGGCTTTCTGCAT+AGG | 0.472012 | 8.1:-58925611 | MS.gene067233:CDS |
| ATGAAATCCCCTGGACTCAA+TGG | 0.472573 | 8.1:+58925575 | None:intergenic |
| GAACATAAGACTTGTGTTGT+CGG | 0.473614 | 8.1:-58926498 | MS.gene067233:CDS |
| ACCGGAGAGGATGATGATAC+TGG | 0.478656 | 8.1:-58926398 | MS.gene067233:CDS |
| CAGATAGGAGAGAGCACAAA+GGG | 0.484810 | 8.1:-58925663 | MS.gene067233:CDS |
| GTGAACAGATGATGATTTCA+AGG | 0.489198 | 8.1:-58925775 | MS.gene067233:intron |
| TTGAGGATGCCATTGAGTCC+AGG | 0.491586 | 8.1:-58925584 | MS.gene067233:CDS |
| ACTTCCCACCTCTGTCTCCA+TGG | 0.494438 | 8.1:+58925191 | None:intergenic |
| TGAAGAAACAAATGAAGATA+GGG | 0.494506 | 8.1:-58925517 | MS.gene067233:CDS |
| GGTGGACAAGGTGGGATACT+GGG | 0.494635 | 8.1:-58925288 | MS.gene067233:CDS |
| AAAACATATCAGCACAAGAA+AGG | 0.502276 | 8.1:+58924889 | None:intergenic |
| CTCATCAGAATCCCACCGCT+TGG | 0.506927 | 8.1:+58925129 | None:intergenic |
| CTGCTTAAGTATATCAATGA+GGG | 0.508162 | 8.1:+58925446 | None:intergenic |
| TCATCCTCTCCGGTTTCAGC+AGG | 0.511272 | 8.1:+58926407 | None:intergenic |
| TTTCAAGGAATTTGTGGATG+GGG | 0.511520 | 8.1:-58925760 | MS.gene067233:CDS |
| GAAGAAGCTAAGGAGCAGAT+AGG | 0.515777 | 8.1:-58925678 | MS.gene067233:CDS |
| GGTGGTGAAGGTGGACAAGG+TGG | 0.521742 | 8.1:-58925297 | MS.gene067233:CDS |
| GCGAGAAAAGCCGTATTGAT+CGG | 0.523585 | 8.1:-58926674 | MS.gene067233:CDS |
| TCCCACATGGAGTGAAGAAT+AGG | 0.524773 | 8.1:-58925485 | MS.gene067233:CDS |
| ACCAGTATCATCATCCTCTC+CGG | 0.525146 | 8.1:+58926397 | None:intergenic |
| CTCCAGCATGGAGGTGGTGA+AGG | 0.526322 | 8.1:-58925309 | MS.gene067233:CDS |
| GGGAAAGAAAATATAGATGT+TGG | 0.529307 | 8.1:-58925417 | MS.gene067233:CDS |
| TGGCTCCGTGTACGATGAAT+CGG | 0.530980 | 8.1:+58926529 | None:intergenic |
| ATTTCAAGGAATTTGTGGAT+GGG | 0.534026 | 8.1:-58925761 | MS.gene067233:CDS |
| TGAAGGGACATGAAATCCCC+TGG | 0.538955 | 8.1:+58925566 | None:intergenic |
| GCATTAGCTGCATTTCCGGC+AGG | 0.539676 | 8.1:+58925069 | None:intergenic |
| GGAGGTGGTGAAGGTGGACA+AGG | 0.539731 | 8.1:-58925300 | MS.gene067233:CDS |
| AAATGCTTGATCGAACGTTA+CGG | 0.540516 | 8.1:-58926587 | MS.gene067233:CDS |
| AACAAGCTCCAGCATGGAGG+TGG | 0.540605 | 8.1:-58925315 | MS.gene067233:CDS |
| AGTAAGCATGAAGTATATGC+TGG | 0.543669 | 8.1:-58925171 | MS.gene067233:CDS |
| TGCAATACAGGCTATAATTG+AGG | 0.544373 | 8.1:-58925028 | MS.gene067233:CDS |
| GATTTCAAGGAATTTGTGGA+TGG | 0.550385 | 8.1:-58925762 | MS.gene067233:CDS |
| TCTGATATGAATCTCATCAC+TGG | 0.553185 | 8.1:-58926353 | MS.gene067233:intron |
| TTTGTTCATTATAGTGGACA+TGG | 0.555229 | 8.1:-58926440 | MS.gene067233:CDS |
| AGAGCGAAGCTACAGAAAGA+TGG | 0.556470 | 8.1:-58924961 | MS.gene067233:CDS |
| TGAGGATGCCATTGAGTCCA+GGG | 0.557608 | 8.1:-58925583 | MS.gene067233:CDS |
| AGGTGGGATACTGGGAATGG+TGG | 0.558174 | 8.1:-58925280 | MS.gene067233:CDS |
| GTCGGAGCTGGTGAGATCAT+CGG | 0.559141 | 8.1:-58926480 | MS.gene067233:CDS |
| AAGCGCGGTCTTCCAAGCGG+TGG | 0.560038 | 8.1:-58925141 | MS.gene067233:CDS |
| AGAGAGCACAAAGGGAGAAG+AGG | 0.560245 | 8.1:-58925655 | MS.gene067233:CDS |
| TGATCACTGCAATAGAGTCC+TGG | 0.562515 | 8.1:+58924925 | None:intergenic |
| AAGACTTGTGTTGTCGGAGC+TGG | 0.562880 | 8.1:-58926492 | MS.gene067233:CDS |
| GTAGTTGATTCCGATCAATA+CGG | 0.563226 | 8.1:+58926664 | None:intergenic |
| CAGCATGGAGGTGGTGAAGG+TGG | 0.564016 | 8.1:-58925306 | MS.gene067233:CDS |
| GCCTGCCATGGAGACAGAGG+TGG | 0.572337 | 8.1:-58925196 | MS.gene067233:CDS |
| GGTGGGATACTGGGAATGGT+GGG | 0.573761 | 8.1:-58925279 | MS.gene067233:CDS |
| AGCGCGGTCTTCCAAGCGGT+GGG | 0.574258 | 8.1:-58925140 | MS.gene067233:CDS |
| GATGGATTCACTCAAAAGCC+AGG | 0.574901 | 8.1:-58924943 | MS.gene067233:CDS |
| CTTTCTGCATAGGAAAGTTG+AGG | 0.574996 | 8.1:-58925601 | MS.gene067233:CDS |
| GGGATATGCAAAGCCTGCCA+TGG | 0.575780 | 8.1:-58925208 | MS.gene067233:CDS |
| ATGGGGTTCCTAGAGGATGT+AGG | 0.576033 | 8.1:-58925743 | MS.gene067233:CDS |
| TGGCTTGATTGAAGAAGCTA+AGG | 0.577289 | 8.1:-58925688 | MS.gene067233:CDS |
| ATACTCGGAGCTTATTCTCA+AGG | 0.577748 | 8.1:-58924986 | MS.gene067233:CDS |
| GTGGTGAAGGTGGACAAGGT+GGG | 0.577935 | 8.1:-58925296 | MS.gene067233:CDS |
| GTGATGAGATTCATATCAGA+AGG | 0.580568 | 8.1:+58926356 | None:intergenic |
| GTATGGCGACGCAATCCTGA+AGG | 0.580869 | 8.1:+58925549 | None:intergenic |
| TCTGCTTAAGTATATCAATG+AGG | 0.583793 | 8.1:+58925445 | None:intergenic |
| TGATGGTGCAATTACATACT+CGG | 0.584306 | 8.1:-58925001 | MS.gene067233:CDS |
| ATGGTCAGCCTACATCCTCT+AGG | 0.591604 | 8.1:+58925735 | None:intergenic |
| ATCTTGAACAAGCTCCAGCA+TGG | 0.591703 | 8.1:-58925321 | MS.gene067233:CDS |
| CTGGTGAGATCATCGGAGGA+TGG | 0.601085 | 8.1:-58926473 | MS.gene067233:CDS |
| ATATACTTAAGCAGAAAACT+GGG | 0.608702 | 8.1:-58925437 | MS.gene067233:CDS |
| GACCTATTCTTCACTCCATG+TGG | 0.610109 | 8.1:+58925483 | None:intergenic |
| TTGTTCATTATAGTGGACAT+GGG | 0.616864 | 8.1:-58926439 | MS.gene067233:CDS |
| GATGCGTAAACGACGTGTGG+AGG | 0.622173 | 8.1:-58926616 | MS.gene067233:CDS |
| AGCGGTGGGATTCTGATGAG+CGG | 0.625209 | 8.1:-58925126 | MS.gene067233:CDS |
| ATATCAATGAGGGTTGAAAG+TGG | 0.625536 | 8.1:+58925456 | None:intergenic |
| GATTGCGTCGCCATACACGC+AGG | 0.630892 | 8.1:-58925542 | MS.gene067233:CDS |
| TCTGATTCATGCCACAGTGG+TGG | 0.633718 | 8.1:-58925708 | MS.gene067233:CDS |
| AAGCTGAATGATAATGACGA+GGG | 0.637692 | 8.1:-58925228 | MS.gene067233:CDS |
| TATGGCGACGCAATCCTGAA+GGG | 0.639983 | 8.1:+58925550 | None:intergenic |
| GCAGATAGGAGAGAGCACAA+AGG | 0.640959 | 8.1:-58925664 | MS.gene067233:CDS |
| GAGGATGCGTAAACGACGTG+TGG | 0.642474 | 8.1:-58926619 | MS.gene067233:CDS |
| ACCTATTCTTCACTCCATGT+GGG | 0.644192 | 8.1:+58925484 | None:intergenic |
| GTGGCATGAATCAGATACAA+TGG | 0.644776 | 8.1:+58925716 | None:intergenic |
| ATATAGATGTTGGTCAACTG+AGG | 0.645754 | 8.1:-58925407 | MS.gene067233:CDS |
| CCTGCCATGGAGACAGAGGT+GGG | 0.647082 | 8.1:-58925195 | MS.gene067233:CDS |
| GCTATAATTGAGGAGACTGA+TGG | 0.648617 | 8.1:-58925018 | MS.gene067233:CDS |
| CGACACCGATTCATCGTACA+CGG | 0.650804 | 8.1:-58926534 | MS.gene067233:CDS |
| TTTGTGGATGGGGTTCCTAG+AGG | 0.655242 | 8.1:-58925750 | MS.gene067233:CDS |
| ATTGCGTCGCCATACACGCA+GGG | 0.657799 | 8.1:-58925541 | MS.gene067233:CDS |
| ATTGATCGGAATCAACTACC+CGG | 0.662029 | 8.1:-58926660 | MS.gene067233:CDS |
| AGGGATATCGAACTCCCACA+TGG | 0.666782 | 8.1:-58925498 | MS.gene067233:CDS |
| AAAGCCTGCCATGGAGACAG+AGG | 0.668348 | 8.1:-58925199 | MS.gene067233:CDS |
| ACAAGGTGGGATACTGGGAA+TGG | 0.670102 | 8.1:-58925283 | MS.gene067233:CDS |
| TTGATCGGAATCAACTACCC+GGG | 0.674290 | 8.1:-58926659 | MS.gene067233:CDS |
| GAGGATGCCATTGAGTCCAG+GGG | 0.680842 | 8.1:-58925582 | MS.gene067233:CDS |
| GGAGCTGGTGAGATCATCGG+AGG | 0.686408 | 8.1:-58926477 | MS.gene067233:CDS |
| TCTTCCTGCTGAAACCGGAG+AGG | 0.694590 | 8.1:-58926411 | MS.gene067233:CDS |
| AAAGCTGAATGATAATGACG+AGG | 0.698351 | 8.1:-58925229 | MS.gene067233:CDS |
| TCAAAGCGCGGTCTTCCAAG+CGG | 0.707343 | 8.1:-58925144 | MS.gene067233:CDS |
| ACAAGAAAGGATCATCAACA+TGG | 0.709272 | 8.1:+58924902 | None:intergenic |
| TTGAACAAGCTCCAGCATGG+AGG | 0.724624 | 8.1:-58925318 | MS.gene067233:CDS |
| TATGCTGGATCGTCAAAGCG+CGG | 0.728471 | 8.1:-58925156 | MS.gene067233:CDS |
| GGAACAAAAGCAGAATTACG+AGG | 0.740123 | 8.1:-58926638 | MS.gene067233:CDS |
| CTTCAATCAAGCCACCACTG+TGG | 0.744555 | 8.1:+58925697 | None:intergenic |
| GTATCTGATTCATGCCACAG+TGG | 0.772525 | 8.1:-58925711 | MS.gene067233:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTTGTAATCTTTATATA+GGG | - | chr8.1:58925710-58925729 | MS.gene067233:CDS | 15.0% |
| !! | AAAAATGAGAGTAGTTTATT+TGG | + | chr8.1:58925253-58925272 | None:intergenic | 20.0% |
| !! | CATGTTTGTAATCTTTATAT+AGG | - | chr8.1:58925709-58925728 | MS.gene067233:CDS | 20.0% |
| !! | TTTAAAAAGTTTCTTGTAGT+TGG | + | chr8.1:58925449-58925468 | None:intergenic | 20.0% |
| !! | TTTAGTATACATGAATTGAA+AGG | - | chr8.1:58925628-58925647 | MS.gene067233:CDS | 20.0% |
| !!! | CTTGATTTTATTTGTTGATA+AGG | - | chr8.1:58925404-58925423 | MS.gene067233:CDS | 20.0% |
| ! | ATATACTTAAGCAGAAAACT+GGG | - | chr8.1:58926137-58926156 | MS.gene067233:intron | 25.0% |
| ! | ATGAAGAAACAAATGAAGAT+AGG | - | chr8.1:58926056-58926075 | MS.gene067233:intron | 25.0% |
| ! | TGAAGAAACAAATGAAGATA+GGG | - | chr8.1:58926057-58926076 | MS.gene067233:intron | 25.0% |
| ! | TTCATAAACTTCTTCACTTT+AGG | + | chr8.1:58926229-58926248 | None:intergenic | 25.0% |
| !!! | CTACACTTTTTGACATTTTT+GGG | - | chr8.1:58926191-58926210 | MS.gene067233:intron | 25.0% |
| !!! | TACACTTTTTGACATTTTTG+GGG | - | chr8.1:58926192-58926211 | MS.gene067233:intron | 25.0% |
| !!! | TCTGTTTTTGTTTTGATCAA+GGG | - | chr8.1:58925288-58925307 | MS.gene067233:CDS | 25.0% |
| ATGAATTGAAAGGAACAGAA+GGG | - | chr8.1:58925638-58925657 | MS.gene067233:CDS | 30.0% | |
| ATGGAGAAAATTCATAGATC+AGG | - | chr8.1:58925520-58925539 | MS.gene067233:CDS | 30.0% | |
| ATGTCATTAGTGAGTAGTAA+TGG | - | chr8.1:58925741-58925760 | MS.gene067233:CDS | 30.0% | |
| ATTTCAAGGAATTTGTGGAT+GGG | - | chr8.1:58925813-58925832 | MS.gene067233:intron | 30.0% | |
| ATTTCGTGTAGAGATAGAAA+TGG | - | chr8.1:58925501-58925520 | MS.gene067233:CDS | 30.0% | |
| CTCGATTAAAATTCGAACAT+TGG | + | chr8.1:58925595-58925614 | None:intergenic | 30.0% | |
| CTGCTTAAGTATATCAATGA+GGG | + | chr8.1:58926131-58926150 | None:intergenic | 30.0% | |
| GATATACTTAAGCAGAAAAC+TGG | - | chr8.1:58926136-58926155 | MS.gene067233:intron | 30.0% | |
| GGGAAAGAAAATATAGATGT+TGG | - | chr8.1:58926157-58926176 | MS.gene067233:intron | 30.0% | |
| TCTGCTTAAGTATATCAATG+AGG | + | chr8.1:58926132-58926151 | None:intergenic | 30.0% | |
| TGATAAGGAATATCCAGATA+CGG | - | chr8.1:58925419-58925438 | MS.gene067233:CDS | 30.0% | |
| TGATGATTTCAAGGAATTTG+TGG | - | chr8.1:58925808-58925827 | MS.gene067233:intron | 30.0% | |
| ! | CCAAAAATGTCAAAAAGTGT+AGG | + | chr8.1:58926193-58926212 | None:intergenic | 30.0% |
| ! | CTTTTGCTTAAGAAACTCTT+GGG | + | chr8.1:58926329-58926348 | None:intergenic | 30.0% |
| !! | CCTACACTTTTTGACATTTT+TGG | - | chr8.1:58926190-58926209 | MS.gene067233:intron | 30.0% |
| !! | TAAAATTCGAACATTGGTTC+CGG | + | chr8.1:58925589-58925608 | None:intergenic | 30.0% |
| !! | TTGTTCATTATAGTGGACAT+GGG | - | chr8.1:58925135-58925154 | MS.gene067233:CDS | 30.0% |
| !! | TTTGTTCATTATAGTGGACA+TGG | - | chr8.1:58925134-58925153 | MS.gene067233:CDS | 30.0% |
| !!! | CTCTGTTTTTGTTTTGATCA+AGG | - | chr8.1:58925287-58925306 | MS.gene067233:CDS | 30.0% |
| AAATGCTTGATCGAACGTTA+CGG | - | chr8.1:58924987-58925006 | MS.gene067233:CDS | 35.0% | |
| ACAAGAAAGGATCATCAACA+TGG | + | chr8.1:58926675-58926694 | None:intergenic | 35.0% | |
| AGTAAGCATGAAGTATATGC+TGG | - | chr8.1:58926403-58926422 | MS.gene067233:CDS | 35.0% | |
| ATAAGCATTAGCTGCATTTC+CGG | + | chr8.1:58926512-58926531 | None:intergenic | 35.0% | |
| ATATAGATGTTGGTCAACTG+AGG | - | chr8.1:58926167-58926186 | MS.gene067233:intron | 35.0% | |
| ATATCAATGAGGGTTGAAAG+TGG | + | chr8.1:58926121-58926140 | None:intergenic | 35.0% | |
| CAAAACAGTGATGTCATCTT+CGG | + | chr8.1:58925018-58925037 | None:intergenic | 35.0% | |
| CATGAATTGAAAGGAACAGA+AGG | - | chr8.1:58925637-58925656 | MS.gene067233:CDS | 35.0% | |
| GAACATAAGACTTGTGTTGT+CGG | - | chr8.1:58925076-58925095 | MS.gene067233:CDS | 35.0% | |
| GATTTCAAGGAATTTGTGGA+TGG | - | chr8.1:58925812-58925831 | MS.gene067233:intron | 35.0% | |
| GTGAACAGATGATGATTTCA+AGG | - | chr8.1:58925799-58925818 | MS.gene067233:intron | 35.0% | |
| GTGATGAGATTCATATCAGA+AGG | + | chr8.1:58925221-58925240 | None:intergenic | 35.0% | |
| TCTGATATGAATCTCATCAC+TGG | - | chr8.1:58925221-58925240 | MS.gene067233:CDS | 35.0% | |
| TGCAATACAGGCTATAATTG+AGG | - | chr8.1:58926546-58926565 | MS.gene067233:CDS | 35.0% | |
| TTTCAAGGAATTTGTGGATG+GGG | - | chr8.1:58925814-58925833 | MS.gene067233:intron | 35.0% | |
| ! | AAAGCTGAATGATAATGACG+AGG | - | chr8.1:58926345-58926364 | MS.gene067233:intron | 35.0% |
| ! | AAGCTGAATGATAATGACGA+GGG | - | chr8.1:58926346-58926365 | MS.gene067233:intron | 35.0% |
| ! | AGTCTTATGTTCTTTCCTGT+TGG | + | chr8.1:58925068-58925087 | None:intergenic | 35.0% |
| ! | GCTTTTGCTTAAGAAACTCT+TGG | + | chr8.1:58926330-58926349 | None:intergenic | 35.0% |
| ! | GTAGTTGATTCCGATCAATA+CGG | + | chr8.1:58924913-58924932 | None:intergenic | 35.0% |
| ! | TGATGGTGCAATTACATACT+CGG | - | chr8.1:58926573-58926592 | MS.gene067233:CDS | 35.0% |
| !! | GTGCTGTTTGTTCATTATAG+TGG | - | chr8.1:58925128-58925147 | MS.gene067233:CDS | 35.0% |
| ACCTATTCTTCACTCCATGT+GGG | + | chr8.1:58926093-58926112 | None:intergenic | 40.0% | |
| AGCTTTCAGCAATGCAATAC+AGG | - | chr8.1:58926534-58926553 | MS.gene067233:CDS | 40.0% | |
| AGGTAAGATTGTCTCCATAG+TGG | - | chr8.1:58925540-58925559 | MS.gene067233:CDS | 40.0% | |
| ATACTCGGAGCTTATTCTCA+AGG | - | chr8.1:58926588-58926607 | MS.gene067233:CDS | 40.0% | |
| ATTGATCGGAATCAACTACC+CGG | - | chr8.1:58924914-58924933 | MS.gene067233:CDS | 40.0% | |
| CTTTCTGCATAGGAAAGTTG+AGG | - | chr8.1:58925973-58925992 | MS.gene067233:intron | 40.0% | |
| GATTCTGGTCAATTCCACTA+TGG | + | chr8.1:58925557-58925576 | None:intergenic | 40.0% | |
| GCTATAATTGAGGAGACTGA+TGG | - | chr8.1:58926556-58926575 | MS.gene067233:CDS | 40.0% | |
| GGAAATGCAGCTAATGCTTA+TGG | - | chr8.1:58926511-58926530 | MS.gene067233:CDS | 40.0% | |
| GGAACAAAAGCAGAATTACG+AGG | - | chr8.1:58924936-58924955 | MS.gene067233:CDS | 40.0% | |
| GTGGCATGAATCAGATACAA+TGG | + | chr8.1:58925861-58925880 | None:intergenic | 40.0% | |
| TGGCTTGATTGAAGAAGCTA+AGG | - | chr8.1:58925886-58925905 | MS.gene067233:intron | 40.0% | |
| TGTAGTTGGAATTCCGTATC+TGG | + | chr8.1:58925435-58925454 | None:intergenic | 40.0% | |
| !! | CGTAATTCTGCTTTTGTTCC+CGG | + | chr8.1:58924936-58924955 | None:intergenic | 40.0% |
| !! | GTAATTCTGCTTTTGTTCCC+GGG | + | chr8.1:58924935-58924954 | None:intergenic | 40.0% |
| AAGGGAGAAGAGGAACATTC+TGG | - | chr8.1:58925929-58925948 | MS.gene067233:intron | 45.0% | |
| ACCAGTATCATCATCCTCTC+CGG | + | chr8.1:58925180-58925199 | None:intergenic | 45.0% | |
| AGAGCGAAGCTACAGAAAGA+TGG | - | chr8.1:58926613-58926632 | MS.gene067233:CDS | 45.0% | |
| ATCTTGAACAAGCTCCAGCA+TGG | - | chr8.1:58926253-58926272 | MS.gene067233:intron | 45.0% | |
| ATGAAATCCCCTGGACTCAA+TGG | + | chr8.1:58926002-58926021 | None:intergenic | 45.0% | |
| GAAGAAGCTAAGGAGCAGAT+AGG | - | chr8.1:58925896-58925915 | MS.gene067233:intron | 45.0% | |
| GAAGAGGAACATTCTGGATC+TGG | - | chr8.1:58925935-58925954 | MS.gene067233:intron | 45.0% | |
| GACCAGAATCTCACAATCTC+CGG | - | chr8.1:58925567-58925586 | MS.gene067233:CDS | 45.0% | |
| GACCTATTCTTCACTCCATG+TGG | + | chr8.1:58926094-58926113 | None:intergenic | 45.0% | |
| GTATCTGATTCATGCCACAG+TGG | - | chr8.1:58925863-58925882 | MS.gene067233:intron | 45.0% | |
| TCCCACATGGAGTGAAGAAT+AGG | - | chr8.1:58926089-58926108 | MS.gene067233:intron | 45.0% | |
| TGATCACTGCAATAGAGTCC+TGG | + | chr8.1:58926652-58926671 | None:intergenic | 45.0% | |
| TTCCGGAGATTGTGAGATTC+TGG | + | chr8.1:58925572-58925591 | None:intergenic | 45.0% | |
| TTGATCGGAATCAACTACCC+GGG | - | chr8.1:58924915-58924934 | MS.gene067233:CDS | 45.0% | |
| TTTCTTCATCCCTGCGTGTA+TGG | + | chr8.1:58926045-58926064 | None:intergenic | 45.0% | |
| ! | CAGATAGGAGAGAGCACAAA+GGG | - | chr8.1:58925911-58925930 | MS.gene067233:intron | 45.0% |
| ! | GAACATTCTGGATCTGGCTT+TGG | - | chr8.1:58925941-58925960 | MS.gene067233:intron | 45.0% |
| ! | GATGGATTCACTCAAAAGCC+AGG | - | chr8.1:58926631-58926650 | MS.gene067233:CDS | 45.0% |
| ! | GCGAGAAAAGCCGTATTGAT+CGG | - | chr8.1:58924900-58924919 | MS.gene067233:CDS | 45.0% |
| !! | GATTGTCTGGCTTTCTGCAT+AGG | - | chr8.1:58925963-58925982 | MS.gene067233:intron | 45.0% |
| AAGACTTGTGTTGTCGGAGC+TGG | - | chr8.1:58925082-58925101 | MS.gene067233:CDS | 50.0% | |
| AAGGAACAGAAGGGAACGTG+TGG | - | chr8.1:58925647-58925666 | MS.gene067233:CDS | 50.0% | |
| ACAAGGTGGGATACTGGGAA+TGG | - | chr8.1:58926291-58926310 | MS.gene067233:intron | 50.0% | |
| ACCGGAGAGGATGATGATAC+TGG | - | chr8.1:58925176-58925195 | MS.gene067233:CDS | 50.0% | |
| ACTCGTCTTCCTGCTGAAAC+CGG | - | chr8.1:58925158-58925177 | MS.gene067233:CDS | 50.0% | |
| AGGGATATCGAACTCCCACA+TGG | - | chr8.1:58926076-58926095 | MS.gene067233:intron | 50.0% | |
| AGGGGATTTCATGTCCCTTC+AGG | - | chr8.1:58926010-58926029 | MS.gene067233:intron | 50.0% | |
| ATGGGGTTCCTAGAGGATGT+AGG | - | chr8.1:58925831-58925850 | MS.gene067233:intron | 50.0% | |
| ATGGTCAGCCTACATCCTCT+AGG | + | chr8.1:58925842-58925861 | None:intergenic | 50.0% | |
| CTTCAATCAAGCCACCACTG+TGG | + | chr8.1:58925880-58925899 | None:intergenic | 50.0% | |
| GACAAGCATCTGCTGAAGTC+TGG | + | chr8.1:58926486-58926505 | None:intergenic | 50.0% | |
| TATGCTGGATCGTCAAAGCG+CGG | - | chr8.1:58926418-58926437 | MS.gene067233:CDS | 50.0% | |
| TATGGCGACGCAATCCTGAA+GGG | + | chr8.1:58926027-58926046 | None:intergenic | 50.0% | |
| TCTGATTCATGCCACAGTGG+TGG | - | chr8.1:58925866-58925885 | MS.gene067233:intron | 50.0% | |
| TGAAGGGACATGAAATCCCC+TGG | + | chr8.1:58926011-58926030 | None:intergenic | 50.0% | |
| TGAGGATGCCATTGAGTCCA+GGG | - | chr8.1:58925991-58926010 | MS.gene067233:intron | 50.0% | |
| TGGCTCCGTGTACGATGAAT+CGG | + | chr8.1:58925048-58925067 | None:intergenic | 50.0% | |
| TTGAACAAGCTCCAGCATGG+AGG | - | chr8.1:58926256-58926275 | MS.gene067233:intron | 50.0% | |
| TTGAGGATGCCATTGAGTCC+AGG | - | chr8.1:58925990-58926009 | MS.gene067233:intron | 50.0% | |
| TTTGTGGATGGGGTTCCTAG+AGG | - | chr8.1:58925824-58925843 | MS.gene067233:intron | 50.0% | |
| ! | AGAGAGCACAAAGGGAGAAG+AGG | - | chr8.1:58925919-58925938 | MS.gene067233:intron | 50.0% |
| ! | GCAGATAGGAGAGAGCACAA+AGG | - | chr8.1:58925910-58925929 | MS.gene067233:intron | 50.0% |
| ! | GGGTTAGTAGAGCTTTCGTC+TGG | - | chr8.1:58925308-58925327 | MS.gene067233:CDS | 50.0% |
| !! | CGACACCGATTCATCGTACA+CGG | - | chr8.1:58925040-58925059 | MS.gene067233:CDS | 50.0% |
| !! | GGATCTGGCTTTGGATTGTC+TGG | - | chr8.1:58925950-58925969 | MS.gene067233:intron | 50.0% |
| AAAGCCTGCCATGGAGACAG+AGG | - | chr8.1:58926375-58926394 | MS.gene067233:CDS | 55.0% | |
| AACAAGCTCCAGCATGGAGG+TGG | - | chr8.1:58926259-58926278 | MS.gene067233:intron | 55.0% | |
| ACTTCCCACCTCTGTCTCCA+TGG | + | chr8.1:58926386-58926405 | None:intergenic | 55.0% | |
| AGCGGTGGGATTCTGATGAG+CGG | - | chr8.1:58926448-58926467 | MS.gene067233:CDS | 55.0% | |
| AGGTGGACAAGGTGGGATAC+TGG | - | chr8.1:58926285-58926304 | MS.gene067233:intron | 55.0% | |
| AGGTGGGATACTGGGAATGG+TGG | - | chr8.1:58926294-58926313 | MS.gene067233:intron | 55.0% | |
| ATTGCGTCGCCATACACGCA+GGG | - | chr8.1:58926033-58926052 | MS.gene067233:intron | 55.0% | |
| CTCATCAGAATCCCACCGCT+TGG | + | chr8.1:58926448-58926467 | None:intergenic | 55.0% | |
| CTGGTGAGATCATCGGAGGA+TGG | - | chr8.1:58925101-58925120 | MS.gene067233:CDS | 55.0% | |
| GAGGATGCCATTGAGTCCAG+GGG | - | chr8.1:58925992-58926011 | MS.gene067233:intron | 55.0% | |
| GAGGATGCGTAAACGACGTG+TGG | - | chr8.1:58924955-58924974 | MS.gene067233:CDS | 55.0% | |
| GATGCGTAAACGACGTGTGG+AGG | - | chr8.1:58924958-58924977 | MS.gene067233:CDS | 55.0% | |
| GCATTAGCTGCATTTCCGGC+AGG | + | chr8.1:58926508-58926527 | None:intergenic | 55.0% | |
| GGGATATGCAAAGCCTGCCA+TGG | - | chr8.1:58926366-58926385 | MS.gene067233:CDS | 55.0% | |
| GGTGGACAAGGTGGGATACT+GGG | - | chr8.1:58926286-58926305 | MS.gene067233:intron | 55.0% | |
| GGTGGGATACTGGGAATGGT+GGG | - | chr8.1:58926295-58926314 | MS.gene067233:intron | 55.0% | |
| GTATGGCGACGCAATCCTGA+AGG | + | chr8.1:58926028-58926047 | None:intergenic | 55.0% | |
| GTCGGAGCTGGTGAGATCAT+CGG | - | chr8.1:58925094-58925113 | MS.gene067233:CDS | 55.0% | |
| GTGGTGAAGGTGGACAAGGT+GGG | - | chr8.1:58926278-58926297 | MS.gene067233:intron | 55.0% | |
| TCAAAGCGCGGTCTTCCAAG+CGG | - | chr8.1:58926430-58926449 | MS.gene067233:CDS | 55.0% | |
| TCAGCAGATGCTTGTCCTGC+CGG | - | chr8.1:58926490-58926509 | MS.gene067233:CDS | 55.0% | |
| TCATCCTCTCCGGTTTCAGC+AGG | + | chr8.1:58925170-58925189 | None:intergenic | 55.0% | |
| TCATCGTACACGGAGCCAAC+AGG | - | chr8.1:58925050-58925069 | MS.gene067233:CDS | 55.0% | |
| TCTTCCTGCTGAAACCGGAG+AGG | - | chr8.1:58925163-58925182 | MS.gene067233:CDS | 55.0% | |
| CACCTTCACCACCTCCATGC+TGG | + | chr8.1:58926270-58926289 | None:intergenic | 60.0% | |
| CAGCATGGAGGTGGTGAAGG+TGG | - | chr8.1:58926268-58926287 | MS.gene067233:intron | 60.0% | |
| CCTGCCATGGAGACAGAGGT+GGG | - | chr8.1:58926379-58926398 | MS.gene067233:CDS | 60.0% | |
| CTCCAGCATGGAGGTGGTGA+AGG | - | chr8.1:58926265-58926284 | MS.gene067233:intron | 60.0% | |
| GATTGCGTCGCCATACACGC+AGG | - | chr8.1:58926032-58926051 | MS.gene067233:intron | 60.0% | |
| GGAGCTGGTGAGATCATCGG+AGG | - | chr8.1:58925097-58925116 | MS.gene067233:CDS | 60.0% | |
| GGAGGTGGTGAAGGTGGACA+AGG | - | chr8.1:58926274-58926293 | MS.gene067233:intron | 60.0% | |
| GGTGGTGAAGGTGGACAAGG+TGG | - | chr8.1:58926277-58926296 | MS.gene067233:intron | 60.0% | |
| AAGCGCGGTCTTCCAAGCGG+TGG | - | chr8.1:58926433-58926452 | MS.gene067233:CDS | 65.0% | |
| AGCGCGGTCTTCCAAGCGGT+GGG | - | chr8.1:58926434-58926453 | MS.gene067233:CDS | 65.0% | |
| CCCACCTCTGTCTCCATGGC+AGG | + | chr8.1:58926382-58926401 | None:intergenic | 65.0% | |
| GCCTGCCATGGAGACAGAGG+TGG | - | chr8.1:58926378-58926397 | MS.gene067233:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 58924897 | 58926699 | 58924897 | ID=MS.gene067233 |
| chr8.1 | mRNA | 58924897 | 58926699 | 58924897 | ID=MS.gene067233.t1;Parent=MS.gene067233 |
| chr8.1 | exon | 58926354 | 58926699 | 58926354 | ID=MS.gene067233.t1.exon1;Parent=MS.gene067233.t1 |
| chr8.1 | CDS | 58926354 | 58926699 | 58926354 | ID=cds.MS.gene067233.t1;Parent=MS.gene067233.t1 |
| chr8.1 | exon | 58924897 | 58925789 | 58924897 | ID=MS.gene067233.t1.exon2;Parent=MS.gene067233.t1 |
| chr8.1 | CDS | 58924897 | 58925789 | 58924897 | ID=cds.MS.gene067233.t1;Parent=MS.gene067233.t1 |
| Gene Sequence |
| Protein sequence |
