AlfalfaGEDB | MS.gene070829

M. sativa cultivar XinJiangDaYe / MS.gene070829

KEGG: K10418
GO: GO:0005575 GO:0005875 GO:0007017 GO:0008150 GO:0009987 GO:0030286 GO:0032991 GO:1902494
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070829.t1	XP_013445572.1	98.2	109	2	0	1	109	1	109	5.70E-54	219.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070829.t1	Q22799	55.1	89	38	1	1	89	1	87	4.6e-20	98.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070829.t1	A0A072TRW8	98.2	109	2	0	1	109	1	109	4.1e-54	219.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene070829	0.8034	3.55E-49	-1.69E-46
MS.gene049577	MS.gene070829	0.834027	3.80E-56	-1.69E-46
MS.gene049910	MS.gene070829	0.803271	3.77E-49	-1.69E-46
MS.gene05137	MS.gene070829	0.814673	1.37E-51	-1.69E-46
MS.gene051983	MS.gene070829	0.823282	1.51E-53	-1.69E-46
MS.gene052077	MS.gene070829	0.848284	6.76E-60	-1.69E-46
MS.gene052309	MS.gene070829	-0.801819	7.52E-49	-1.69E-46
MS.gene052340	MS.gene070829	0.800606	1.33E-48	-1.69E-46
MS.gene053073	MS.gene070829	0.831486	1.62E-55	-1.69E-46
MS.gene053836	MS.gene070829	0.803856	2.85E-49	-1.69E-46
MS.gene054930	MS.gene070829	0.838653	2.53E-57	-1.69E-46
MS.gene05553	MS.gene070829	0.807666	4.52E-50	-1.69E-46
MS.gene056979	MS.gene070829	0.82256	2.22E-53	-1.69E-46
MS.gene05702	MS.gene070829	0.807078	6.02E-50	-1.69E-46
MS.gene058142	MS.gene070829	0.823114	1.65E-53	-1.69E-46
MS.gene059285	MS.gene070829	0.830012	3.73E-55	-1.69E-46
MS.gene059511	MS.gene070829	0.820115	8.14E-53	-1.69E-46
MS.gene059894	MS.gene070829	0.816172	6.34E-52	-1.69E-46
MS.gene059899	MS.gene070829	0.807233	5.59E-50	-1.69E-46
MS.gene061049	MS.gene070829	0.831171	1.94E-55	-1.69E-46
MS.gene061159	MS.gene070829	0.817596	3.04E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene070829	MS.gene78481	PPI
MS.gene070829	MS.gene041599	PPI
MS.gene070829	MS.gene041598	PPI
MS.gene035362	MS.gene070829	PPI
MS.gene070829	MS.gene77822	PPI
MS.gene070829	MS.gene035363	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070829.t1	MTR_8g464690	98.165	109	2	0	1	109	1	109	1.41e-76	221
MS.gene070829.t1	MTR_6g087770	51.087	92	45	0	1	92	1	92	4.05e-30	103
MS.gene070829.t1	MTR_2g101850	55.556	90	40	0	1	90	1	90	1.97e-29	101
MS.gene070829.t1	MTR_4g120150	48.837	86	42	1	5	90	46	129	7.92e-26	94.0
MS.gene070829.t1	MTR_4g114060	49.367	79	40	0	11	89	159	237	1.16e-21	85.9
MS.gene070829.t1	MTR_8g104900	45.882	85	44	1	12	94	45	129	4.08e-19	76.6
MS.gene070829.t1	MTR_1g031880	43.529	85	46	1	12	94	59	143	7.24e-19	76.6
MS.gene070829.t1	MTR_4g107350	50.633	79	38	1	12	89	222	300	3.36e-18	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070829.t1	AT4G27360	70.874	103	30	0	1	103	1	103	1.27e-49	153
MS.gene070829.t1	AT3G16120	55.435	92	41	0	1	92	1	92	8.47e-33	110
MS.gene070829.t1	AT3G16120	55.435	92	41	0	1	92	1	92	8.47e-33	110
MS.gene070829.t1	AT1G52245	51.064	94	46	0	1	94	1	94	3.96e-30	103
MS.gene070829.t1	AT4G15930	49.412	85	41	1	5	89	39	121	7.17e-24	88.6
MS.gene070829.t1	AT5G20110	46.988	83	44	0	12	94	123	205	5.05e-21	83.6
MS.gene070829.t1	AT1G23220	39.773	88	52	1	8	94	38	125	5.50e-18	73.9
MS.gene070829.t1	AT4G15930	49.180	61	29	1	5	65	39	97	1.81e-13	61.2

Find 22 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCATTGTAGGCACTGATTT+TGG	0.221609	8.1:+49310913	MS.gene070829:CDS
CAGGAATTTGATAGGGTTCA+TGG	0.298316	8.1:+49310877	MS.gene070829:intron
CAGTTACATCATAGAAATCT+AGG	0.300776	8.1:-49310732	None:intergenic
AATTATTGCAGGAATTTGAT+AGG	0.304941	8.1:+49310869	MS.gene070829:intron
ATCATAGAAATCTAGGGCTT+TGG	0.353606	8.1:-49310725	None:intergenic
CTTCAATTCATCATGCTAGA+AGG	0.354098	8.1:+49310637	None:intergenic
TTGCTCTTTAAGGGATCTGC+TGG	0.420352	8.1:+49310997	MS.gene070829:CDS
CCTGGTTGGCAATGCATTGT+AGG	0.440795	8.1:+49310901	MS.gene070829:CDS
CTAGAAGGCAAGGCAGTGAT+TGG	0.481296	8.1:+49310652	MS.gene070829:CDS
CCTACAATGCATTGCCAACC+AGG	0.504627	8.1:-49310901	None:intergenic
AAATCAGTTCTCAGCAATAG+AGG	0.512873	8.1:+49311035	MS.gene070829:CDS
TATAGCTCGATTTATAAAGA+AGG	0.514762	8.1:+49310765	MS.gene070829:CDS
TTTGATAGGGTTCATGGACC+TGG	0.522418	8.1:+49310883	MS.gene070829:CDS
ATTATTGCAGGAATTTGATA+GGG	0.524356	8.1:+49310870	MS.gene070829:intron
TATAAATCGAGCTATATCAA+TGG	0.561045	8.1:-49310758	None:intergenic
ATCTGCTGGTCCACAAGCAG+AGG	0.571115	8.1:+49311011	MS.gene070829:CDS
ATTCATCATGCTAGAAGGCA+AGG	0.581622	8.1:+49310642	None:intergenic
TCCTTTGTGACACATTGTAG+TGG	0.583602	8.1:+49310937	MS.gene070829:CDS
AGTTACATCATAGAAATCTA+GGG	0.585718	8.1:-49310731	None:intergenic
ATAGGGTTCATGGACCTGGT+TGG	0.594187	8.1:+49310887	MS.gene070829:CDS
GCCACTACAATGTGTCACAA+AGG	0.659746	8.1:-49310938	None:intergenic
AGATTTCTATGATGTAACTG+AGG	0.680263	8.1:+49310735	MS.gene070829:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GGTAATAAAATAAAACTAAG+AGG	+	chr8.1:49310786-49310805	MS.gene070829:intron	20.0%
!	AAACTAAGAGGATTGTTTAT+TGG	+	chr8.1:49310798-49310817	MS.gene070829:intron	25.0%
!	AATTATTGCAGGAATTTGAT+AGG	+	chr8.1:49310869-49310888	MS.gene070829:intron	25.0%
!	AGTTACATCATAGAAATCTA+GGG	-	chr8.1:49310734-49310753	None:intergenic	25.0%
!	ATGTGTTAGTTAATTATTGC+AGG	+	chr8.1:49310858-49310877	MS.gene070829:intron	25.0%
!	ATTATTGCAGGAATTTGATA+GGG	+	chr8.1:49310870-49310889	MS.gene070829:intron	25.0%
!	TATAAATCGAGCTATATCAA+TGG	-	chr8.1:49310761-49310780	None:intergenic	25.0%
!	TATAGCTCGATTTATAAAGA+AGG	+	chr8.1:49310765-49310784	MS.gene070829:CDS	25.0%
!!!	CTATTTTTGTATAGGTAGTA+TGG	+	chr8.1:49310969-49310988	MS.gene070829:CDS	25.0%
!!!	TGCTTCATCTATTTTTGTAT+AGG	+	chr8.1:49310961-49310980	MS.gene070829:CDS	25.0%
	AGATTTCTATGATGTAACTG+AGG	+	chr8.1:49310735-49310754	MS.gene070829:CDS	30.0%
	CAGTTACATCATAGAAATCT+AGG	-	chr8.1:49310735-49310754	None:intergenic	30.0%
!	ATGGCAATTTTGCTCTTTAA+GGG	+	chr8.1:49310988-49311007	MS.gene070829:CDS	30.0%
!	TATGGCAATTTTGCTCTTTA+AGG	+	chr8.1:49310987-49311006	MS.gene070829:CDS	30.0%
	AAATCAGTTCTCAGCAATAG+AGG	+	chr8.1:49311035-49311054	MS.gene070829:CDS	35.0%
!	ATCATAGAAATCTAGGGCTT+TGG	-	chr8.1:49310728-49310747	None:intergenic	35.0%
	CAGGAATTTGATAGGGTTCA+TGG	+	chr8.1:49310877-49310896	MS.gene070829:intron	40.0%
	TCCTTTGTGACACATTGTAG+TGG	+	chr8.1:49310937-49310956	MS.gene070829:CDS	40.0%
!!	AACTGATTTTCCTCTGCTTG+TGG	-	chr8.1:49311024-49311043	None:intergenic	40.0%
!!!	TGCATTGTAGGCACTGATTT+TGG	+	chr8.1:49310913-49310932	MS.gene070829:CDS	40.0%
	GCCACTACAATGTGTCACAA+AGG	-	chr8.1:49310941-49310960	None:intergenic	45.0%
	TTTGATAGGGTTCATGGACC+TGG	+	chr8.1:49310883-49310902	MS.gene070829:CDS	45.0%
!	TTGCTCTTTAAGGGATCTGC+TGG	+	chr8.1:49310997-49311016	MS.gene070829:CDS	45.0%
	ATAGGGTTCATGGACCTGGT+TGG	+	chr8.1:49310887-49310906	MS.gene070829:CDS	50.0%
	CCTACAATGCATTGCCAACC+AGG	-	chr8.1:49310904-49310923	None:intergenic	50.0%
	CCTGGTTGGCAATGCATTGT+AGG	+	chr8.1:49310901-49310920	MS.gene070829:CDS	50.0%
	CTAGAAGGCAAGGCAGTGAT+TGG	+	chr8.1:49310652-49310671	MS.gene070829:CDS	50.0%
	ATCTGCTGGTCCACAAGCAG+AGG	+	chr8.1:49311011-49311030	MS.gene070829:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	49310649	49311071	49310649	ID=MS.gene070829
chr8.1	mRNA	49310649	49311071	49310649	ID=MS.gene070829.t1;Parent=MS.gene070829
chr8.1	exon	49310649	49310786	49310649	ID=MS.gene070829.t1.exon1;Parent=MS.gene070829.t1
chr8.1	CDS	49310649	49310786	49310649	ID=cds.MS.gene070829.t1;Parent=MS.gene070829.t1
chr8.1	exon	49310880	49311071	49310880	ID=MS.gene070829.t1.exon2;Parent=MS.gene070829.t1
chr8.1	CDS	49310880	49311071	49310880	ID=cds.MS.gene070829.t1;Parent=MS.gene070829.t1

Gene Sequence

>MS.gene070829

ATGCTAGAAGGCAAGGCAGTGATTGGTGAGACTGACATGCTTCATACTATGCAACAAGATGCTCTTGATTTAGCATCCAAAGCCCTAGATTTCTATGATGTAACTGAGGCCATTGATATAGCTCGATTTATAAAGAAGGTAATAAAATAAAACTAAGAGGATTGTTTATTGGTTATTTTAATGTAATTTTTTTTATAGACAAATTTTTGATGTGTTAGTTAATTATTGCAGGAATTTGATAGGGTTCATGGACCTGGTTGGCAATGCATTGTAGGCACTGATTTTGGTTCCTTTGTGACACATTGTAGTGGCTGCTTCATCTATTTTTGTATAGGTAGTATGGCAATTTTGCTCTTTAAGGGATCTGCTGGTCCACAAGCAGAGGAAAATCAGTTCTCAGCAATAGAGGCAACTAAAGCATAA

Protein sequence

>MS.gene070829.t1

MLEGKAVIGETDMLHTMQQDALDLASKALDFYDVTEAIDIARFIKKEFDRVHGPGWQCIVGTDFGSFVTHCSGCFIYFCIGSMAILLFKGSAGPQAEENQFSAIEATKA