Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070831.t1 | XP_013445572.1 | 98.2 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 5.70E-54 | 219.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070831.t1 | Q22799 | 55.1 | 89 | 38 | 1 | 1 | 89 | 1 | 87 | 4.6e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070831.t1 | A0A072TRW8 | 98.2 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 4.1e-54 | 219.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene070831 | 0.816211 | 6.21E-52 | -1.69E-46 |
| MS.gene049577 | MS.gene070831 | 0.824649 | 7.20E-54 | -1.69E-46 |
| MS.gene049910 | MS.gene070831 | 0.809122 | 2.21E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene070831 | 0.801325 | 9.50E-49 | -1.69E-46 |
| MS.gene05137 | MS.gene070831 | 0.82191 | 3.14E-53 | -1.69E-46 |
| MS.gene051983 | MS.gene070831 | 0.831723 | 1.42E-55 | -1.69E-46 |
| MS.gene052077 | MS.gene070831 | 0.857769 | 1.30E-62 | -1.69E-46 |
| MS.gene052309 | MS.gene070831 | -0.81301 | 3.18E-51 | -1.69E-46 |
| MS.gene053073 | MS.gene070831 | 0.831812 | 1.35E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene070831 | 0.808122 | 3.62E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene070831 | 0.84787 | 8.79E-60 | -1.69E-46 |
| MS.gene05553 | MS.gene070831 | 0.80508 | 1.59E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene070831 | 0.807013 | 6.22E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene070831 | 0.827987 | 1.15E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene070831 | 0.818339 | 2.06E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene070831 | 0.831299 | 1.81E-55 | -1.69E-46 |
| MS.gene059511 | MS.gene070831 | 0.827659 | 1.38E-54 | -1.69E-46 |
| MS.gene059894 | MS.gene070831 | 0.817237 | 3.66E-52 | -1.69E-46 |
| MS.gene059899 | MS.gene070831 | 0.809535 | 1.80E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene070831 | 0.827347 | 1.64E-54 | -1.69E-46 |
| MS.gene061159 | MS.gene070831 | 0.805237 | 1.47E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070831 | MS.gene035363 | PPI |
| MS.gene070831 | MS.gene77822 | PPI |
| MS.gene035362 | MS.gene070831 | PPI |
| MS.gene070831 | MS.gene78481 | PPI |
| MS.gene041599 | MS.gene070831 | PPI |
| MS.gene070831 | MS.gene041598 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070831.t1 | MTR_8g464690 | 98.165 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 1.41e-76 | 221 |
| MS.gene070831.t1 | MTR_6g087770 | 51.087 | 92 | 45 | 0 | 1 | 92 | 1 | 92 | 4.05e-30 | 103 |
| MS.gene070831.t1 | MTR_2g101850 | 55.556 | 90 | 40 | 0 | 1 | 90 | 1 | 90 | 1.97e-29 | 101 |
| MS.gene070831.t1 | MTR_4g120150 | 48.837 | 86 | 42 | 1 | 5 | 90 | 46 | 129 | 7.92e-26 | 94.0 |
| MS.gene070831.t1 | MTR_4g114060 | 49.367 | 79 | 40 | 0 | 11 | 89 | 159 | 237 | 1.16e-21 | 85.9 |
| MS.gene070831.t1 | MTR_8g104900 | 45.882 | 85 | 44 | 1 | 12 | 94 | 45 | 129 | 4.08e-19 | 76.6 |
| MS.gene070831.t1 | MTR_1g031880 | 43.529 | 85 | 46 | 1 | 12 | 94 | 59 | 143 | 7.24e-19 | 76.6 |
| MS.gene070831.t1 | MTR_4g107350 | 50.633 | 79 | 38 | 1 | 12 | 89 | 222 | 300 | 3.36e-18 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070831.t1 | AT4G27360 | 70.874 | 103 | 30 | 0 | 1 | 103 | 1 | 103 | 1.27e-49 | 153 |
| MS.gene070831.t1 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 8.47e-33 | 110 |
| MS.gene070831.t1 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 8.47e-33 | 110 |
| MS.gene070831.t1 | AT1G52245 | 51.064 | 94 | 46 | 0 | 1 | 94 | 1 | 94 | 3.96e-30 | 103 |
| MS.gene070831.t1 | AT4G15930 | 49.412 | 85 | 41 | 1 | 5 | 89 | 39 | 121 | 7.17e-24 | 88.6 |
| MS.gene070831.t1 | AT5G20110 | 46.988 | 83 | 44 | 0 | 12 | 94 | 123 | 205 | 5.05e-21 | 83.6 |
| MS.gene070831.t1 | AT1G23220 | 39.773 | 88 | 52 | 1 | 8 | 94 | 38 | 125 | 5.50e-18 | 73.9 |
| MS.gene070831.t1 | AT4G15930 | 49.180 | 61 | 29 | 1 | 5 | 65 | 39 | 97 | 1.81e-13 | 61.2 |
Find 22 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTAGGCACTGATTT+TGG | 0.221609 | 8.1:+49335613 | MS.gene070831:CDS |
| CAGGAATTTGATAGGGTTCA+TGG | 0.298316 | 8.1:+49335577 | MS.gene070831:intron |
| CAGTTACATCATAGAAATCT+AGG | 0.300776 | 8.1:-49335432 | None:intergenic |
| AATTATTGCAGGAATTTGAT+AGG | 0.304941 | 8.1:+49335569 | MS.gene070831:intron |
| ATCATAGAAATCTAGGGCTT+TGG | 0.353606 | 8.1:-49335425 | None:intergenic |
| CTTCAATTCATCATGCTAGA+AGG | 0.354098 | 8.1:+49335337 | None:intergenic |
| TTGCTCTTTAAGGGATCTGC+TGG | 0.420352 | 8.1:+49335697 | MS.gene070831:CDS |
| CCTGGTTGGCAATGCATTGT+AGG | 0.440795 | 8.1:+49335601 | MS.gene070831:CDS |
| CTAGAAGGCAAGGCAGTGAT+TGG | 0.481296 | 8.1:+49335352 | MS.gene070831:CDS |
| CCTACAATGCATTGCCAACC+AGG | 0.504627 | 8.1:-49335601 | None:intergenic |
| AAATCAGTTCTCAGCAATAG+AGG | 0.512873 | 8.1:+49335735 | MS.gene070831:CDS |
| TATAGCTCGATTTATAAAGA+AGG | 0.514762 | 8.1:+49335465 | MS.gene070831:CDS |
| TTTGATAGGGTTCATGGACC+TGG | 0.522418 | 8.1:+49335583 | MS.gene070831:CDS |
| ATTATTGCAGGAATTTGATA+GGG | 0.524356 | 8.1:+49335570 | MS.gene070831:intron |
| TATAAATCGAGCTATATCAA+TGG | 0.561045 | 8.1:-49335458 | None:intergenic |
| ATCTGCTGGTCCACAAGCAG+AGG | 0.571115 | 8.1:+49335711 | MS.gene070831:CDS |
| ATTCATCATGCTAGAAGGCA+AGG | 0.581622 | 8.1:+49335342 | None:intergenic |
| TCCTTTGTGACACATTGTAG+TGG | 0.583602 | 8.1:+49335637 | MS.gene070831:CDS |
| AGTTACATCATAGAAATCTA+GGG | 0.585718 | 8.1:-49335431 | None:intergenic |
| ATAGGGTTCATGGACCTGGT+TGG | 0.594187 | 8.1:+49335587 | MS.gene070831:CDS |
| GCCACTACAATGTGTCACAA+AGG | 0.659746 | 8.1:-49335638 | None:intergenic |
| AGATTTCTATGATGTAACTG+AGG | 0.680263 | 8.1:+49335435 | MS.gene070831:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTAATAAAATAAAACTAAG+AGG | + | chr8.1:49335486-49335505 | MS.gene070831:intron | 20.0% |
| ! | AAACTAAGAGGATTGTTTAT+TGG | + | chr8.1:49335498-49335517 | MS.gene070831:intron | 25.0% |
| ! | AATTATTGCAGGAATTTGAT+AGG | + | chr8.1:49335569-49335588 | MS.gene070831:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | - | chr8.1:49335434-49335453 | None:intergenic | 25.0% |
| ! | ATGTGTTAGTTAATTATTGC+AGG | + | chr8.1:49335558-49335577 | MS.gene070831:intron | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | + | chr8.1:49335570-49335589 | MS.gene070831:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | - | chr8.1:49335461-49335480 | None:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | + | chr8.1:49335465-49335484 | MS.gene070831:CDS | 25.0% |
| !!! | CTATTTTTGTATAGGTAGTA+TGG | + | chr8.1:49335669-49335688 | MS.gene070831:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | + | chr8.1:49335661-49335680 | MS.gene070831:CDS | 25.0% |
| AGATTTCTATGATGTAACTG+AGG | + | chr8.1:49335435-49335454 | MS.gene070831:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | - | chr8.1:49335435-49335454 | None:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | + | chr8.1:49335688-49335707 | MS.gene070831:CDS | 30.0% |
| ! | TATGGCAATTTTGCTCTTTA+AGG | + | chr8.1:49335687-49335706 | MS.gene070831:CDS | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | + | chr8.1:49335735-49335754 | MS.gene070831:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | - | chr8.1:49335428-49335447 | None:intergenic | 35.0% |
| CAGGAATTTGATAGGGTTCA+TGG | + | chr8.1:49335577-49335596 | MS.gene070831:intron | 40.0% | |
| TCCTTTGTGACACATTGTAG+TGG | + | chr8.1:49335637-49335656 | MS.gene070831:CDS | 40.0% | |
| !! | AACTGATTTTCCTCTGCTTG+TGG | - | chr8.1:49335724-49335743 | None:intergenic | 40.0% |
| !!! | TGCATTGTAGGCACTGATTT+TGG | + | chr8.1:49335613-49335632 | MS.gene070831:CDS | 40.0% |
| GCCACTACAATGTGTCACAA+AGG | - | chr8.1:49335641-49335660 | None:intergenic | 45.0% | |
| TTTGATAGGGTTCATGGACC+TGG | + | chr8.1:49335583-49335602 | MS.gene070831:CDS | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | + | chr8.1:49335697-49335716 | MS.gene070831:CDS | 45.0% |
| ATAGGGTTCATGGACCTGGT+TGG | + | chr8.1:49335587-49335606 | MS.gene070831:CDS | 50.0% | |
| CCTACAATGCATTGCCAACC+AGG | - | chr8.1:49335604-49335623 | None:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | + | chr8.1:49335601-49335620 | MS.gene070831:CDS | 50.0% | |
| CTAGAAGGCAAGGCAGTGAT+TGG | + | chr8.1:49335352-49335371 | MS.gene070831:CDS | 50.0% | |
| ATCTGCTGGTCCACAAGCAG+AGG | + | chr8.1:49335711-49335730 | MS.gene070831:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 49335349 | 49335771 | 49335349 | ID=MS.gene070831 |
| chr8.1 | mRNA | 49335349 | 49335771 | 49335349 | ID=MS.gene070831.t1;Parent=MS.gene070831 |
| chr8.1 | exon | 49335349 | 49335486 | 49335349 | ID=MS.gene070831.t1.exon1;Parent=MS.gene070831.t1 |
| chr8.1 | CDS | 49335349 | 49335486 | 49335349 | ID=cds.MS.gene070831.t1;Parent=MS.gene070831.t1 |
| chr8.1 | exon | 49335580 | 49335771 | 49335580 | ID=MS.gene070831.t1.exon2;Parent=MS.gene070831.t1 |
| chr8.1 | CDS | 49335580 | 49335771 | 49335580 | ID=cds.MS.gene070831.t1;Parent=MS.gene070831.t1 |
| Gene Sequence |
| Protein sequence |
